CN112226474A - Preparation method of D-psicose crystal - Google Patents
Preparation method of D-psicose crystal Download PDFInfo
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- CN112226474A CN112226474A CN202011237164.8A CN202011237164A CN112226474A CN 112226474 A CN112226474 A CN 112226474A CN 202011237164 A CN202011237164 A CN 202011237164A CN 112226474 A CN112226474 A CN 112226474A
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- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/24—Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
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Abstract
The invention discloses a preparation method of D-psicose crystals, which comprises the steps of firstly taking 45-50% g/g D-fructose solution, adding 8-12U/ml D-psicose 3-epimerase, reacting at 50-60 ℃ to obtain 40-50wt% sugar-concentrated enzyme conversion solution, then sequentially carrying out high-concentration decolorization, conductivity reduction by ion exchange resin, chromatographic column separation by a sequential simulated moving bed chromatographic column, high-concentration decolorization, conductivity reduction by ion exchange resin, high-concentration cooling crystallization, finally carrying out vacuum concentration, slowly cooling until crystals are separated out after seed crystals are added, and drying the separated crystals in a fluidized bed and an aging bin. The method has simple operation and low temperature in the process, can maintain the stability of the product to the maximum extent, obtains the final product with high quality, and has larger industrial production and application value.
Description
Technical Field
The invention belongs to the technical field of preparation of D-psicose, and particularly relates to a preparation method of a D-psicose crystal.
Background
D-psicose belongs to rare monosaccharide and is a low-calorie sweetener, and if a 100g/L sucrose solution is taken as an example, the sweetness of the D-psicose is 70% of sucrose, but only 0.3% of the sucrose energy is the best substitute of the sucrose in food. Meanwhile, the D-psicose can inhibit liver lipogenic enzyme and intestinal alpha-glycosidase in vivo, so that the D-psicose is difficult to be metabolized and absorbed in small intestine, can reduce postprandial hyperglycemia, improve insulin resistance and reduce accumulation of fat in vivo, has unique physiological function, and is beneficial to obesity and diabetes patients. In addition, the U.S. Food and Drug Administration (FDA) announces that D-psicose is a "generally recognized as safe" (GRAS) food and is applicable to the fields of food, medicine, and the like. With the increasing living standard and the increasing emphasis on food safety, the sweetener D-psicose with various functional properties and low calorie will receive high attention from consumers.
D-psicose mainly has two routes of biosynthesis and chemical synthesis. Compared with chemical synthesis, the biosynthesis method is more environment-friendly, is beneficial to separation and purification of subsequent products, and has great economic and social benefits. Korea Xijie first sugar manufacturing company, Japan Songgu group and England Tailey company are three major manufacturers currently producing D-psicose globally, all of which produce D-psicose 3-epimerase by improved recombinant strains and produce D-psicose by using fructose catalyzed by the enzyme, and commercialization of D-psicose has been achieved. Compared with foreign countries, the research on D-psicose in China is relatively lagged. A rhodobacter sphaeroides strain capable of synthesizing D-psicose 3-epimerase is obtained by screening sludge and water samples in a fish pond at first by Jiangnan university, and the rhodobacter sphaeroides strain can catalyze D-fructose to generate D-psicose, and the conversion rate is up to 6.54%. However, the biological method has certain disadvantages, and the existing enzymatic method for producing D-psicose is mainly limited by low catalytic efficiency of the used enzyme, large substrate viscosity, complicated production process and the like.
Chinese patent CN106520863A discloses, for example, a process for preparing D-psicose by converting D-fructose with a biological enzyme, which comprises using D-fructose as a raw material, subjecting the D-psicose to 3-epimerase on fructose, decolorizing, ion-exchange, concentrating, chromatographic separation and crystallizing to obtain D-psicose crystals, wherein the conversion rate of the fructose into D-psicose is low (below 30%) and the yield of the crystals after crystallization is low. Chinese patent CN109306365A discloses a method for preparing D-psicose powder by vacuum spray drying, which is characterized in that instant spray drying is completed under a low temperature condition, but the spray drying is easy to block, and the product is easy to agglomerate in the storage process, so that the production efficiency is low.
In conclusion, it is important to develop a method for preparing high-purity and high-quality D-psicose.
Disclosure of Invention
Aiming at the problem of low yield of D-psicose crystals in the prior art, the invention provides the preparation method of the D-psicose crystals, which is simple to operate, low in process temperature, capable of keeping the stability of the products to the maximum extent, capable of obtaining high-quality final products and high in industrial production and application value.
The invention is realized by the following technical scheme:
a preparation method of D-psicose crystals comprises the following steps:
(1) enzyme conversion: taking 45-50% g/g D-fructose solution, adding 8-12U/ml D-psicose 3-epimerase, reacting at 50-60 ℃ for 8-10 h, heating and inactivating enzyme to obtain enzyme conversion solution;
(2) and (3) high-concentration decolorization: carrying out high-concentration decolorization on the enzyme conversion solution in the step (1);
(3) high-concentration ion exchange: reducing the conductance of the decolored solution in the step (2) through ion exchange resin at the speed of 2-3 BV/h;
(4) continuous chromatographic separation: the enzyme conversion solution after high-concentration ion exchange in the step (3) enters a sequential simulated moving bed chromatographic column for chromatographic separation;
(5) and (3) high-concentration decolorization: decoloring the enzymatic solution subjected to continuous chromatographic separation in the step (4) again;
(6) high-concentration ion exchange: the enzyme conversion solution decolorized in the step (5) reduces the electric conductivity by ion exchange resin at the speed of 2-3 BV/h;
(7) high-concentration cooling crystallization: carrying out vacuum concentration on the enzyme conversion solution subjected to high-concentration ion exchange in the step (6) to obtain a D-psicose concentrated solution, rapidly cooling to 40-50 ℃, adding 60-80 mesh psicose seed crystals under stirring, and slowly cooling until crystals are separated out;
(8) centrifugal drying: and (3) centrifugally separating the concentrated solution containing the crystals, washing with water, and drying the obtained wet crystals in a fluidized bed and an aging bin until the moisture content is lower than 0.05%.
Further, the sugar concentration in the enzyme conversion solution obtained in the step (1) is 40-50wt% of feed liquid; the enzyme activity of the D-psicose 3-epimerase is 1000-2000U/ml.
Further, the high-concentration decolorization in the step (2) and the step (5) is granular carbon decolorization, the decolorization temperature is 70-80 ℃, and the decolorization time is 2-4 h.
Further, the temperature for high concentration ion exchange in the step (3) and the step (6) is 50-60 ℃, and the pressure is 0.3-0.4 MPa.
Further, the ion exchange resin in the step (3) and the step (6) is cation and anion exchange resin.
Further, the temperature of the chromatographic separation in the step (4) is 70-80 ℃, and the separation speed is 1.0 ml/min.
Further, the concentration of the D-psicose concentrated solution in the step (7) is 80-85 wt% of the total sugar concentration of the feed liquid; the seed crystal is 60-80 mesh allulose crystal, the stirring speed is 150-.
Further, the adding amount of the seed crystal in the step (7) is 0.1-1% of the dry basis mass of the vacuum concentrated solution.
Further, the centrifugal separation conditions in step (8) are 4000-.
Advantageous effects
The invention provides a D-psicose crystal with high purity and high yield, the method is simple to operate, the process is low in temperature, the stability of the product can be kept to the maximum extent, a high-quality final product is obtained, and the method has great industrial production and application values.
Detailed Description
The method of operation of the present invention is further illustrated below with reference to specific examples. These examples are only for illustrating the present invention in detail and are not intended to limit the scope of the present invention.
The fructose source in the invention is a product of biological GmbH of Baoling (bowling) with the purity of 99.8%.
Example 1
(1) Enzyme conversion: adding D-psicose 3-epimerase (the enzyme activity of the D-psicose 3-epimerase is 1150U/ml) into a 45wt% D-fructose solution according to the amount of 8U/ml fructose, reacting at 55 ℃ for 9 h, heating to 100 ℃ to inactivate enzyme to obtain an enzyme conversion solution, wherein the sugar concentration in the enzyme conversion solution is 45 wt%;
(2) and (3) high-concentration decolorization: adding the enzyme conversion solution obtained in the step (1) into a granular carbon column for decoloring, wherein the decoloring temperature is 75 ℃, the decoloring time is 3h, and the light transmittance of the enzyme conversion solution after decoloring is (T)440nm）98.3%；
(3) High-concentration ion exchange: the enzyme conversion solution after the high concentration decolorization in the step (2) passes through cation (D001) anion (D301) ion exchange resin at the speed of 3BV/h to reduce the electric conductivity, the temperature is 55 ℃, the pressure is 0.3MPa, the electric conductivity of the obtained feed liquid is 40.5 mu s/cm, and the light transmittance (T) is440nm) 99.3 percent and the pH value is 6.2;
(4) continuous chromatographic separation: separating the enzyme conversion solution subjected to high-concentration ion exchange in the step (3) by a sequential simulated moving bed chromatographic column at the separation speed of 1.0ml/min and the temperature of 70 ℃, wherein the purity of D-psicose in the enzyme conversion solution is 98.5% after chromatographic separation;
(5) and (3) high-concentration decolorization: the enzyme conversion solution obtained after the chromatographic separation in the step (4) enters a granular carbon column again for decolorization, the decolorization temperature is 75 ℃, the decolorization time is 3 hours, and the light transmittance (T) of the enzyme conversion solution after the decolorization is realized440nm) 99.5%;
(6) high-concentration ion exchange: the enzyme conversion solution decolorized again in the step (5) passes through cation (D001) anion (D301) ion exchange resin at the speed of 3BV/h to reduce the electric conductivity, the temperature is 55 ℃, the pressure is 0.3MPa, the electric conductivity of the obtained feed liquid is 20.5 mu s/cm, and the light transmittance (T) is440nm) 99.8 percent and the pH value is 6.3;
(7) high-concentration cooling crystallization: concentrating the enzyme conversion solution after high-concentration ion exchange in the step (6) to 82wt% in vacuum to obtain a D-psicose concentrated solution, rapidly cooling to 48 ℃, adding 0.8% of seed crystal at 160rpm, cooling to 0.18 ℃ per hour, and cooling to 35 ℃;
(8) centrifugal drying: separating D-psicose concentrated solution containing crystals under the centrifugal condition of 5000rpm, wherein the centrifugal time is 30min, washing the crystals after centrifugation (cleaning according to the water addition amount of 15% dry crystals), drying the obtained wet crystals in a fluidized bed and an aging bin, controlling the air inlet humidity to be less than or equal to 25% RH, controlling the air inlet temperature to be 25 ℃, and the aging time to be 25h, controlling the water content of the D-psicose to be 0.03wt% after drying, obtaining the D-psicose with the yield of 68%, and controlling the proportion of crystals with more than 60 meshes to be 60.5%.
Example 2
(1) Enzyme conversion: enzyme conversion: adding D-psicose 3-epimerase (the enzyme activity of the D-psicose 3-epimerase is 1050U/ml) into a D-fructose solution with the concentration of 50wt% according to the amount of 9U/ml, reacting at 55 ℃ for 10 h, heating to 100 ℃ to inactivate enzyme to obtain an enzyme conversion solution, wherein the sugar concentration in the enzyme conversion solution is 50 wt%;
(2) and (3) high-concentration decolorization: adding the enzyme conversion solution obtained in the step (1) into a granular carbon column for decoloring, wherein the decoloring temperature is 80 ℃, the decoloring time is 4 hours, and the light transmittance of the enzyme conversion solution after decoloring is (T)440nm）98.9%；
(3) High-concentration ion exchange: enzyme conversion after high-concentration decolorization in step (2)The chemical solution is passed through cation (D001) anion (D301) ion exchange resin at 2BV/h to reduce electric conductivity, the temperature is 60 deg.C, the pressure is 0.4MPa, the electric conductivity of the obtained material solution is 28.5 mus/cm, and the light transmittance (T) is440nm) 99.5 percent and the pH value is 6.5;
(4) continuous chromatographic separation: separating the enzyme conversion solution subjected to high-concentration ion exchange in the step (3) by a sequential simulated moving bed chromatographic column at a separation speed of 1.0ml/min and a separation temperature of 75 ℃, wherein the purity of D-psicose in the enzyme conversion solution is 99.1% after chromatographic separation;
(5) and (3) high-concentration decolorization: the enzyme conversion solution after the chromatographic separation in the step (4) enters a granular carbon column again for decolorization, the decolorization temperature is 80 ℃, the decolorization time is 4 hours, and the light transmittance (T) of the enzyme conversion solution after the decolorization is realized440nm) 99.8 percent;
(6) high-concentration ion exchange: the enzyme conversion solution decolorized again in the step (5) passes through cation (D001) anion (D301) ion exchange resin at the speed of 2BV/h to reduce the electric conductivity, the temperature is 60 ℃, the pressure is 0.4MPa, the electric conductivity of the obtained feed liquid is 10.3 mu s/cm, and the light transmittance (T) is440nm) 99.8 percent and the pH value is 6.45;
(7) high-concentration cooling crystallization: concentrating the enzyme conversion solution after high-concentration ion exchange in the step (6) to 84wt% in vacuum to obtain a D-psicose concentrated solution, rapidly cooling to 46 ℃, adding 0.8% of seed crystal at 240rpm, cooling to 0.1 ℃ per hour, and cooling to 33 ℃;
(8) centrifugal drying: separating D-psicose concentrated solution containing crystals under the centrifugal condition of 4000rpm, wherein the centrifugal time is 50min, washing the crystals after centrifugation (cleaning according to the water addition amount of 20% of dry crystals), drying the obtained wet crystals in a fluidized bed and an aging bin, controlling the air inlet humidity to be less than or equal to 25% RH, controlling the air inlet temperature to be 20 ℃, and aging for 30 h. The moisture content of the dried D-psicose was 0.02wt%, and the yield of the D-psicose crystals was 71.5%, and the proportion of crystals having a size of 60 mesh or more was 68.9%.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (9)
1. A preparation method of D-psicose crystals is characterized by comprising the following steps:
(1) enzyme conversion: taking 45-50% g/g D-fructose solution, adding 8-12U/ml D-psicose 3-epimerase, reacting at 50-60 ℃ for 8-10 h, heating and inactivating enzyme to obtain enzyme conversion solution;
(2) and (3) high-concentration decolorization: carrying out high-concentration decolorization on the enzyme conversion solution in the step (1);
(3) high-concentration ion exchange: reducing the conductance of the decolored solution in the step (2) through ion exchange resin at the speed of 2-3 BV/h;
(4) continuous chromatographic separation: the enzyme conversion solution after high-concentration ion exchange in the step (3) enters a sequential simulated moving bed chromatographic column for chromatographic separation;
(5) and (3) high-concentration decolorization: decoloring the enzymatic solution subjected to continuous chromatographic separation in the step (4) again;
(6) high-concentration ion exchange: the enzyme conversion solution decolorized in the step (5) reduces the electric conductivity by ion exchange resin at the speed of 2-3 BV/h;
(7) high-concentration cooling crystallization: carrying out vacuum concentration on the enzyme conversion solution subjected to high-concentration ion exchange in the step (6) to obtain a D-psicose concentrated solution, rapidly cooling to 40-50 ℃, adding 60-80 mesh psicose seed crystals under stirring, and slowly cooling until crystals are separated out;
(8) centrifugal drying: and (3) centrifugally separating the concentrated solution containing the crystals, washing with water, and drying the obtained wet crystals in a fluidized bed and an aging bin until the moisture content is lower than 0.05%.
2. The production method according to claim 1, wherein the sugar concentration in the enzyme-converted liquid obtained in step (1) is 40 to 50wt% of the feed liquid; the enzyme activity of the D-psicose 3-epimerase is 1000-2000U/ml.
3. The preparation method according to claim 1, wherein the high-concentration decolorization in the step (2) and the step (5) is granular carbon decolorization, the decolorization temperature is 70-80 ℃, and the decolorization time is 2-4 h.
4. The process according to claim 1, wherein the high concentration ion-exchange in the steps (3) and (6) is carried out at a temperature of 50 to 60 ℃ and a pressure of 0.3 to 0.4 MPa.
5. The method according to claim 1, wherein the cation-anion exchange resin in the steps (3) and (6) is a cation-anion exchange resin.
6. The process according to claim 1, wherein the temperature of the chromatographic separation in the step (4) is 70 to 80 ℃ and the separation rate is 1.0 ml/min.
7. The preparation method according to claim 1, wherein the concentration of the D-psicose concentrated solution in the step (7) is 80-85 wt% of the total sugar concentration of the feed liquid; the seed crystal is 60-80 mesh allulose crystal, the stirring speed is 150-.
8. The method according to claim 7, wherein the seed crystal in the step (7) is added in an amount of 0.1 to 1% by mass on a dry basis of the vacuum concentrated solution.
9. The method as claimed in claim 1, wherein the centrifugation in step (8) is carried out under 4000-5000rpm for 30-50 min.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113080357A (en) * | 2021-05-17 | 2021-07-09 | 江苏赛威分离科技有限公司 | Low-calorie compound sweetener and production process thereof |
| CN113912655A (en) * | 2021-09-30 | 2022-01-11 | 中粮营养健康研究院有限公司 | Method for separating psicose from mixed syrup by using simulated moving bed |
| CN115028667A (en) * | 2022-06-10 | 2022-09-09 | 山东星光首创生物科技有限公司 | Preparation method of psicose with low water activity and psicose |
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| CN110627847A (en) * | 2019-09-17 | 2019-12-31 | 山东百龙创园生物科技股份有限公司 | Preparation method of psicose crystal |
| CN110951806A (en) * | 2019-12-24 | 2020-04-03 | 山东百龙创园生物科技股份有限公司 | Preparation process of D-psicose-containing crystalline composition |
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| CN106520863A (en) * | 2016-11-22 | 2017-03-22 | 保龄宝生物股份有限公司 | Production technology for preparing D-psicose through enzymatic transesferification of D-fructose |
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