CN105603022A - Method for preparing high maltose oligosaccharide - Google Patents
Method for preparing high maltose oligosaccharide Download PDFInfo
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- CN105603022A CN105603022A CN201610055322.5A CN201610055322A CN105603022A CN 105603022 A CN105603022 A CN 105603022A CN 201610055322 A CN201610055322 A CN 201610055322A CN 105603022 A CN105603022 A CN 105603022A
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- fructus hordei
- hordei germinatus
- germinatus oligose
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- maltotriose
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/22—Preparation of compounds containing saccharide radicals produced by the action of a beta-amylase, e.g. maltose
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/12—Disaccharides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/16—Preparation of compounds containing saccharide radicals produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin
Abstract
The invention provides a method for preparing high maltose oligosaccharide. The method includes the following steps of a, saccharifying, wherein after starch is liquefied, a sugar solution is saccharified through the combined effect of maltose trisaccharidase, barley beta-amylase and pullulanase to generate a product with the malt sugar content of about 40% and the maltotriose content of about 40%; b, chromatography separating and purifying, wherein the saccharified and concentrated sugar solution is purified with the chromatographic separation technology, and the mass fraction of feeding ranges from 55% to 60%; under the condition of the running temperature of 60 DEG C of a system, the malt sugar, the maltotriose and glucose are separated, and a feed solution with the component (the maltotriose and the malt sugar) content of 92% or above is obtained; c, refining, wherein the obtained high maltose oligosaccharide solution is subjected to activated carbon decoloring filtering, ion exchange desalting and vacuum concentrating, and the high maltose oligosaccharide product is obtained. The prepared high maltose oligosaccharide has the advantages of being high in purity, excellent in hygroscopicity and moisturizing performance, low in sweetness, good in heat resistance and acid resistance and the like.
Description
Technical field
The invention belongs to starch sugar derivative preparing technical field, be specifically related to a kind of preparation technology of high Fructus Hordei Germinatus oligose.
Background technology
The carbohydrate that starch or maltodextrin per molecule are hydrolyzed into 3-8 molecule monose claims malto-oligosaccharide, also has people hydrolysisBecome 3-10, even the carbohydrate of 20 molecule monose comes within the category. What be made up of three glucose molecules is Fructus Hordei Germinatus threeSugar, what be made up of two glucose molecules is maltose, and maltose and maltotriose are collectively referred to as high Fructus Hordei Germinatus oligose, are all α, D-Glucose is with α-1 → 4 and α-1 → 6 glycosidic bond combination. High Fructus Hordei Germinatus oligose is advanced as raw material adopts to refine cornstarchBiotechnology and a kind of novel sugared source that integrates health care, nutrition of making.
The maximum feature of high Fructus Hordei Germinatus oligose is directly not enter by the digestion of stomach in small intestine, by small intestinal mucosa epithelial cellAfter α-1 → 4 glycosidase on middle microvillose membrane and α-1 → 6 glycosidase fast hydrolyzing, enter rapid glycogen biosynthesis in blood; SimultaneouslyIt is directly drunk afterwards and blood glucose value is sharply raise in motion unlike carbohydrate such as glucose, thereby gives sportsman's bodyBody brings discomfort. High Fructus Hordei Germinatus oligose has the characteristic of low sugariness, low in calories, Hyposmolality, and human body can be held after taking in enteron aisleContinuous hydrolysis, has the effect that extends organ dysfunction, strengthening body endurance and capacity for work. Preventing that a large amount of lactic acid from producing and blood is solidifyingWhen knot, can be absorbed gradually and constantly provide for a long time energy by human body. Therefore, it can strengthen body endurance rapidly,Improve immunity of organisms and capacity for work, thereby overcome the robber that human body occurs after a large amount of physical demands and long-time strenuous exerciseThe symptom such as sweat, dehydration.
High Fructus Hordei Germinatus oligose has the features such as the energy supply time of prolongation, strengthening body endurance and capacity for work and absorption easy to digest, can answerFor pancreatectomy patient's dietary therapy, nephrotic's energy source etc., be particularly useful for baby food. High Fructus Hordei Germinatus oligoseBlood sugar level can maintain human-body fatigue time, avoids a large amount of generations of lactic acid in the concentrated and blood of blood, and the body that can increase sharply is resistance toPower and capacity for work, increase work efficiency. Except tool health care, its sugariness is low, mouthfeel good, moisture retention is good, be difficult for crystallization,Can be applicable to various beverages, sweet wine, senior candy etc., is domestic and international generally acknowledged functional food base-material, can be widely used in health careThe field such as food, beverage, its product consumption amount increases progressively rapidly. Along with improving constantly of China's living standards of the people, become graduallyA larger consumption market, product has a extensive future.
Chinese patent document CN103014097A (application number: 201210471187.4) disclose a kind of wheat taking starch as raw materialBud trisaccharide preparation method and special fungal alpha-amylase thereof, belong to starch sugar manufacture and technical field of enzyme engineering. Form sediment with each PlantsOpaque is raw material, utilizes fungal alpha-amylase SfA to liquefy and saccharification, obtains maltotriose and accounts for total reducing sugar ratio 46%-55%Syrup; Account for total reducing sugar ratio by charcoal absorption and alcohol wash-out acquisition maltotriose and be not less than 90% syrup. This techniqueWeak point is that follow-up charcoal absorption and alcohol elution process are too complicated, are unfavorable for suitability for industrialized production and environmental protection.
Summary of the invention
For the deficiencies in the prior art, the invention provides a kind of preparation method of high Fructus Hordei Germinatus oligose.
Technical scheme of the present invention is as follows:
A preparation method for high Fructus Hordei Germinatus oligose, comprises the following steps:
A) saccharification: the liquid glucose after starch liquefacation is raw through maltotriose enzyme, barley beta-amylase and Pullulanase synergy saccharificationBecome maltose and the maltotriose gross mass content product at 35-85%;
B) chromatographic purification: the product after saccharification is concentrated, then adopt chromatography separating method to purify, by maltose, Fructus Hordei GerminatusTrisaccharide separates with glucose, obtains the high Fructus Hordei Germinatus oligose feed liquid of maltotriose and maltose gross mass content >=92%;
C) refining: the high Fructus Hordei Germinatus oligose feed liquid obtaining, through decolorizing with activated carbon filtration, ion-exchange desalination, Vacuum Concentration, is obtainedTo high Fructus Hordei Germinatus oligose product.
According to the present invention, preferred, step a) in the method for starch liquefacation as follows:
Starch, under the effect of ɑ-alpha-amylase, is generated to micromolecular dextrin;
Further preferred liquifying method is: regulate starch concentration at 16-17 Baume (Be), regulate pH=5.5-6.5, temperature90 DEG C-110 DEG C, add ɑ-alpha-amylase, after liquefaction reaction, starch generates micromolecular dextrin; Preferably, ɑ-GaoThe diastatic addition of temperature is 150mL/T.DS;
According to the present invention, preferred, step a) middle maltotriose enzyme addition is 600mL/T.DS, barley beta-amylase additionFor 150mL/T.DS, Pullulanase addition are 300mL/T.DS;
Preferably, saccharification condition is: pH=4.5-5.1, temperature 58-60 DEG C.
According to the present invention, preferred, step b) in product after saccharification to be concentrated into quality of material concentration be 55-60%;
Preferably, described chromatography separating method is macroreticular resin separation method; Further preferred, charging mass fraction 55-60%, 60 DEG C of system running temperatures;
Preferably, in described macroreticular resin separation method, macroreticular resin is strong base anion resins or low-acid cationic resin, entersThe preferred strong base negative resin D730 of one step;
According to the present invention, preferred, the chromatography separating method of step described in b) is: taking low-acid cationic resin as separation resin,Feeding temperature is 35-40 DEG C, taking two-pass reverse osmosis water as eluant, eluent, crosses post, obtains the high Fructus Hordei Germinatus oligose after purifying and is rich in little pointThe component of sub-carbohydrate.
According to the present invention, preferred, the ion exchange desalination method of step described in c) is: exchange with strong-acid cation successivelyResin, weak basic anion exchange resin and strong-acid cation-exchange resin carry out ion-exchange desalination.
Beneficial effect of the present invention:
The component of the high Fructus Hordei Germinatus oligose (maltose and maltotriose) that 1, the inventive method makes is high, and purity is high, little molecule contentFew.
2, the high Fructus Hordei Germinatus oligose that the inventive method makes is difficult to be digested by gastric enzyme, and sugariness is low, and heat is low, does not substantially increase blood sugarBlood fat; Heat-resisting, acid resistance is splendid, concentration 50% syrup long-time heating under pH=3,120 DEG C can not be decomposed; There is guarantorMoist, make moisture be difficult for evaporation, the moisturizing to various food and its quality maintained good effect, performance is more stable, toolThere are the extensive scope of application and application prospect. After being absorbed by the body, have the energy supply time of prolongation, strengthening body endurance and capacity for work withAnd the feature such as absorption easy to digest, the blood sugar level can maintain human-body fatigue time, avoids the large volume production of lactic acid in the concentrated and blood of bloodRaw, can increase sharply body endurance and capacity for work, increase work efficiency.
3, the present invention adopts the collaborative critical process of maltotriose enzyme, barley beta-amylase and Pullulanase three enzymes to prepare high-purityHigh Fructus Hordei Germinatus oligose, product content is up to more than 80%, and after chromatographic isolation, content reaches more than 90%.
Detailed description of the invention
To be described further the present invention by embodiment below, the description of these embodiment is not that content of the present invention is doneLimit. One skilled in the art will understand that the replacement that is equal to that content of the present invention is done, or improve accordingly, still belong to thisWithin the protection domain of invention.
In embodiment, strong acid cation resin used is D006, and weak base anion resins is SD330.
Embodiment 1
A preparation method for high Fructus Hordei Germinatus oligose, comprises the following steps:
A) taking starch as raw material, regulate starch concentration at 16-17 Baume, regulate pH=5.5,90 DEG C of temperature, add ɑ-high temperature to form sedimentPowder enzyme, after liquefaction reaction, starch generates micromolecular dextrin; The addition of ɑ-alpha-amylase is 150mL/T.DS
By liquid glucose after starch liquefacation through maltotriose enzyme, barley beta-amylase and Pullulanase synergy saccharification generate maltose andMaltotriose content is at the product of 40% left and right; Maltotriose enzyme 450mL/T.DS, barley beta-amylase 150mL/T.DS and generalThe addition of the blue enzyme in Shandong is 300mL/T.DS, and saccharification condition is: pH=4.5-5.1, temperature 58-60 DEG C;
B) adopt chromatographic separation technology to purify liquid glucose concentrated after saccharification, charging mass fraction 55%, 60 DEG C of system running temperaturesUnder condition, maltose, maltotriose are separated with glucose, obtain component at (maltotriose+maltose) more than 92% materialLiquid;
C) the high Fructus Hordei Germinatus oligose feed liquid obtaining is added to 3 ‰ (butt mass ratio in powder activated carbon and feed liquid) powder activated carbon, stirMix evenly, 70 DEG C of maintenance 30min of constant temperature decolour, and then adopt plate and frame filter press filtration of active charcoal, by the filtrate after filteringCarry out ion-exchange desalination;
To the positive post of strong acid cation resin and the cloudy post of weak base anion resins be housed respectively, adopt conventional method to process regeneration.Then by the feed liquid after decolorization filtering with 3 times of resin volumes/hour flow velocity, at 35 DEG C successively by positive post-cloudy post-positive post,Carry out from handing over desalination;
The feed liquid of crossing after post is adopted to quadruple effect falling film evaporator, lead≤100us/cm of discharging pH4-7 electricity,, obtain high Fructus Hordei Germinatus oligoseProduct. Detect through high performance liquid chromatography, the content that detects maltotriose and maltose by National Standard Method is 92%.
Embodiment 2
A preparation method for high Fructus Hordei Germinatus oligose, comprises the following steps:
A) taking starch as raw material, regulate starch concentration at 16-17 Baume, regulate pH=6.0,100 DEG C of temperature, add ɑ-high temperatureAmylase, after liquefaction reaction, starch generates micromolecular dextrin; The addition of ɑ-alpha-amylase is 150mL/T.DS
By liquid glucose after starch liquefacation through maltotriose enzyme, barley beta-amylase and Pullulanase synergy saccharification generate maltose andMaltotriose content is at the product of 40% left and right; Maltotriose enzyme 600mL/T.DS, barley beta-amylase 150mL/T.DS and generalThe addition of the blue enzyme in Shandong is 300mL/T.DS, and saccharification condition is: pH=pH=4.5-5.1, temperature 58-60 DEG C;
B) adopt chromatographic separation technology to purify liquid glucose concentrated after saccharification, charging mass fraction 60%, 60 DEG C of system running temperaturesUnder condition, maltose, maltotriose are separated with glucose, obtain component at (maltotriose+maltose) more than 92% materialLiquid;
C) the high Fructus Hordei Germinatus oligose feed liquid obtaining is added to 3 ‰ (butt mass ratio in powder activated carbon and feed liquid) powder activated carbon, stirMix evenly, 75 DEG C of maintenance 30min of constant temperature decolour, and then adopt plate and frame filter press filtration of active charcoal, by the filtrate after filteringCarry out ion-exchange desalination;
To the positive post of strong acid cation resin and the cloudy post of weak base anion resins be housed respectively, adopt conventional method to process regeneration.Then by the feed liquid after decolorization filtering with 3 times of resin volumes/hour flow velocity, at 40 DEG C successively by positive post-cloudy post-positive post,Carry out from handing over desalination;
The feed liquid of crossing after post is adopted to quadruple effect falling film evaporator, lead≤100us/cm of discharging pH4-7 electricity,, obtain high Fructus Hordei Germinatus oligoseProduct. Detect through high performance liquid chromatography, the content that detects maltotriose and maltose by National Standard Method is 95%.
Embodiment 3
A preparation method for high Fructus Hordei Germinatus oligose, comprises the following steps:
A) taking starch as raw material, regulate starch concentration at 16-17 Baume, regulate pH=6.5,110 DEG C of temperature, add ɑ-high temperatureAmylase, after liquefaction reaction, starch generates micromolecular dextrin; The addition of ɑ-alpha-amylase is 150mL/T.DSLiquid glucose after starch liquefacation is generated to maltose and Fructus Hordei Germinatus through maltotriose enzyme, barley beta-amylase and Pullulanase synergy saccharificationTrisaccharide content is at the product of 40% left and right; Maltotriose enzyme 450mL/T.DS, barley beta-amylase 150mL/T.DS and PropiramThe addition of enzyme is 600mL/T.DS, and saccharification condition is: pH=pH=4.5-5.1, temperature 58-60 DEG C;
B) adopt chromatographic separation technology to purify liquid glucose concentrated after saccharification, charging mass fraction 60%, 60 DEG C of system running temperaturesUnder condition, maltose, maltotriose are separated with glucose, obtain component at (maltotriose+maltose) more than 92% materialLiquid;
C) the high Fructus Hordei Germinatus oligose feed liquid obtaining is added to 3 ‰ (butt mass ratio in powder activated carbon and feed liquid) powder activated carbon, stirMix evenly, 80 DEG C of maintenance 30min of constant temperature decolour, and then adopt plate and frame filter press filtration of active charcoal, by the filtrate after filteringCarry out ion-exchange desalination;
To the positive post of strong acid cation resin and the cloudy post of weak base anion resins be housed respectively, adopt conventional method to process regeneration.Then by the feed liquid after decolorization filtering with 3 times of resin volumes/hour flow velocity, at 40 DEG C successively by positive post-cloudy post-positive post,Carry out from handing over desalination;
The feed liquid of crossing after post is adopted to quadruple effect falling film evaporator, lead≤100us/cm of discharging pH4-7 electricity,, obtain high Fructus Hordei Germinatus oligoseProduct. Detect through high performance liquid chromatography, the content that detects maltotriose and maltose by National Standard Method is 92.5%.
Comparative example
A preparation method for high Fructus Hordei Germinatus oligose, comprises the following steps:
A) taking starch as raw material, regulate starch concentration at 16-17 Baume, regulate pH=6.0,100 DEG C of temperature, add ɑ-high temperatureAmylase, after liquefaction reaction, starch generates micromolecular dextrin; The addition of ɑ-alpha-amylase is 150mL/T.DS
By liquid glucose after starch liquefacation through maltotriose enzyme, barley beta-amylase and Pullulanase synergy saccharification generate maltose andMaltotriose content is at the product of 40% left and right; Maltotriose enzyme 600mL/T.DS, barley beta-amylase 150mL/T.DS and generalThe addition of the blue enzyme in Shandong is 300mL/T.DS, and saccharification condition is: pH=pH=4.5-5.1, temperature 58-60 DEG C;
B) adopt chromatographic separation technology to purify liquid glucose concentrated after saccharification, charging mass fraction 45%, 50 DEG C of system running temperaturesUnder condition, maltose, maltotriose are separated with glucose, obtain component at (maltotriose+maltose) more than 92% materialLiquid;
C) the high Fructus Hordei Germinatus oligose feed liquid obtaining is added to 3 ‰ (butt mass ratio in powder activated carbon and feed liquid) powder activated carbon, stirMix evenly, 75 DEG C of maintenance 30min of constant temperature decolour, and then adopt plate and frame filter press filtration of active charcoal, by the filtrate after filteringCarry out ion-exchange desalination;
To the positive post of strong acid cation resin and the cloudy post of weak base anion resins be housed respectively, adopt conventional method to process regeneration.Then by the feed liquid after decolorization filtering with 3 times of resin volumes/hour flow velocity, at 40 DEG C successively by positive post-cloudy post-positive post,Carry out from handing over desalination;
The feed liquid of crossing after post is adopted to quadruple effect falling film evaporator, lead≤100us/cm of discharging pH=4-7 electricity,, obtain high Fructus Hordei Germinatus oligoseProduct. Detect through high performance liquid chromatography, the content that detects maltotriose and maltose by National Standard Method is 85.5%.
Claims (10)
1. a preparation method for high Fructus Hordei Germinatus oligose, comprises the following steps:
A) saccharification: the liquid glucose after starch liquefacation is raw through maltotriose enzyme, barley beta-amylase and Pullulanase synergy saccharificationBecome maltose and the maltotriose gross mass content product at 35-85%;
B) chromatographic purification: the product after saccharification is concentrated, then adopt chromatography separating method to purify, by maltose, Fructus Hordei GerminatusTrisaccharide separates with glucose, obtains the high Fructus Hordei Germinatus oligose feed liquid of maltotriose and maltose gross mass content >=92%;
C) refining: the high Fructus Hordei Germinatus oligose feed liquid obtaining, through decolorizing with activated carbon filtration, ion-exchange desalination, Vacuum Concentration, is obtainedTo high Fructus Hordei Germinatus oligose product.
2. the preparation method of high Fructus Hordei Germinatus oligose according to claim 1, is characterized in that, step a) middle liquifying method is:Regulate starch concentration at 16-17 Baume (Be), regulate pH=5.5-6.5,90 DEG C-110 DEG C of temperature, add ɑ-alpha-amylase,After liquefaction reaction, starch generates micromolecular dextrin.
3. the preparation method of high Fructus Hordei Germinatus oligose according to claim 2, is characterized in that, the addition of ɑ-alpha-amylaseFor 150mL/T.DS.
4. the preparation method of high Fructus Hordei Germinatus oligose according to claim 1, is characterized in that, step a) middle maltotriose enzyme addsEntering amount is that 600mL/T.DS, barley beta-amylase addition are that 150mL/T.DS, Pullulanase addition are 300mL/T.DS.
5. the preparation method of high Fructus Hordei Germinatus oligose according to claim 1, is characterized in that, step a) middle saccharification condition is:PH=4.5-5.1, temperature 58-60 DEG C.
6. the preparation method of high Fructus Hordei Germinatus oligose according to claim 1, is characterized in that, step b) in product after saccharificationBeing concentrated into quality of material concentration is 55-60%.
7. the preparation method of high Fructus Hordei Germinatus oligose according to claim 1, is characterized in that, the chromatogram of step described in b) dividedBe macroreticular resin separation method from method; Preferably, charging mass fraction 55-60%, 60 DEG C of system running temperatures.
8. the preparation method of high Fructus Hordei Germinatus oligose according to claim 1, is characterized in that, the macropore tree of step described in b)In fat separation method, macroreticular resin is strong base anion resins or low-acid cationic resin.
9. the preparation method of high Fructus Hordei Germinatus oligose according to claim 1, is characterized in that, the chromatogram of step described in b) dividedFrom method be: taking low-acid cationic resin as separation resin, feeding temperature is 35-40 DEG C, taking two-pass reverse osmosis water as eluant, eluent,Cross post, obtain high Fructus Hordei Germinatus oligose and the component that is rich in small molecular sugar class after purifying.
10. the preparation method of high Fructus Hordei Germinatus oligose according to claim 1, is characterized in that, the ion of step described in c)Exchange desalination process is: use successively strong-acid cation-exchange resin, weak basic anion exchange resin and strong-acid cation-exchange resinCarry out ion-exchange desalination.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107653278A (en) * | 2017-10-31 | 2018-02-02 | 无锡甜丰食品有限公司 | A kind of production technology of maltodextrin |
| CN113215208A (en) * | 2021-05-26 | 2021-08-06 | 江苏省奥谷生物科技有限公司 | Preparation method of maltose powder with high maltose content |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101684505A (en) * | 2008-09-24 | 2010-03-31 | 郸城财鑫糖业有限责任公司 | Preparation method of maltose |
| CN101701236A (en) * | 2009-12-10 | 2010-05-05 | 安徽农业大学 | Preparation method of ultra-high maltose syrup |
| CN103014097A (en) * | 2012-11-20 | 2013-04-03 | 江南大学 | Maltotriose preparation method with starch as raw material and special fungal alpha-amylase thereof |
| CN103215326A (en) * | 2013-04-24 | 2013-07-24 | 山东百龙创园生物科技有限公司 | High-purity maltotriose preparation method |
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- 2016-01-27 CN CN201610055322.5A patent/CN105603022A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101684505A (en) * | 2008-09-24 | 2010-03-31 | 郸城财鑫糖业有限责任公司 | Preparation method of maltose |
| CN101701236A (en) * | 2009-12-10 | 2010-05-05 | 安徽农业大学 | Preparation method of ultra-high maltose syrup |
| CN103014097A (en) * | 2012-11-20 | 2013-04-03 | 江南大学 | Maltotriose preparation method with starch as raw material and special fungal alpha-amylase thereof |
| CN103215326A (en) * | 2013-04-24 | 2013-07-24 | 山东百龙创园生物科技有限公司 | High-purity maltotriose preparation method |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107653278A (en) * | 2017-10-31 | 2018-02-02 | 无锡甜丰食品有限公司 | A kind of production technology of maltodextrin |
| CN113215208A (en) * | 2021-05-26 | 2021-08-06 | 江苏省奥谷生物科技有限公司 | Preparation method of maltose powder with high maltose content |
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