CN112218890A - 在疾病和发展中基于纳米抗体的ecm成像和靶向 - Google Patents

在疾病和发展中基于纳米抗体的ecm成像和靶向 Download PDF

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Publication number
CN112218890A
CN112218890A CN201980019075.5A CN201980019075A CN112218890A CN 112218890 A CN112218890 A CN 112218890A CN 201980019075 A CN201980019075 A CN 201980019075A CN 112218890 A CN112218890 A CN 112218890A
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Prior art keywords
ecm
epitope
gly
cells
nanobody
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CN201980019075.5A
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Chinese (zh)
Inventor
R.O.海因斯
N.杰尔卡尼
H.L.普洛格
Y.J.谢
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Boston Childrens Hospital
Massachusetts Institute of Technology
Whitehead Institute for Biomedical Research
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Boston Childrens Hospital
Massachusetts Institute of Technology
Whitehead Institute for Biomedical Research
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Publication of CN112218890A publication Critical patent/CN112218890A/zh
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024040658A1 (zh) * 2022-08-26 2024-02-29 南京融捷康生物科技有限公司 一种抗trem2的单域抗体及其用途

Families Citing this family (8)

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Publication number Priority date Publication date Assignee Title
CN112218890A (zh) 2018-01-25 2021-01-12 麻省理工学院 在疾病和发展中基于纳米抗体的ecm成像和靶向
EP4047016A1 (en) 2021-02-19 2022-08-24 Université de Strasbourg Anti-tenascin-c (tnc) single-domain antibodies (nanobodies) and use thereof
CN113151177B (zh) * 2021-05-21 2023-11-03 四川大学华西医院 乳腺或乳腺癌组织脱细胞基质及其制备方法和用途
US20240238451A1 (en) * 2021-05-28 2024-07-18 Albert Einstein College Of Medicine Single-domain antigen-dependent antibody-like fusion proteins
CN119095870A (zh) * 2022-03-21 2024-12-06 香港理工大学 肽双特异性抗体及其制备方法和应用
IL319909A (en) * 2022-12-30 2025-05-01 Remegen Shanghai Co Ltd Bispecific antibody and its use
WO2025149667A1 (en) 2024-01-12 2025-07-17 Pheon Therapeutics Ltd Antibody drug conjugates and uses thereof
WO2025175132A1 (en) * 2024-02-15 2025-08-21 Board Of Regents, The University Of Texas System Methods for selecting triple-negative breast cancer patients for targeted therapy using tumor-specific total mrna expression

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130136744A1 (en) * 2002-11-08 2013-05-30 Ablynx N.V. Pulmonary administration of immunoglobulin single variable domains and constructs thereof
WO2014145252A2 (en) * 2013-03-15 2014-09-18 Milone Michael C Targeting cytotoxic cells with chimeric receptors for adoptive immunotherapy
WO2017194782A2 (en) * 2016-05-13 2017-11-16 Orionis Biosciences Nv Therapeutic targeting of non-cellular structures

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000076456A2 (en) * 1999-06-14 2000-12-21 The General Hospital Corporation Modulators of fibrosis
CA2723842A1 (en) * 2008-05-09 2009-11-12 Peter Vanlandschoot Amino acid sequences directed against integrins and uses thereof
MX2019000641A (es) * 2016-07-15 2019-06-10 Poseida Therapeutics Inc Composiciones de mucina1-receptor de antigeno quimerico y metodos para su uso.
CN112218890A (zh) 2018-01-25 2021-01-12 麻省理工学院 在疾病和发展中基于纳米抗体的ecm成像和靶向

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130136744A1 (en) * 2002-11-08 2013-05-30 Ablynx N.V. Pulmonary administration of immunoglobulin single variable domains and constructs thereof
WO2014145252A2 (en) * 2013-03-15 2014-09-18 Milone Michael C Targeting cytotoxic cells with chimeric receptors for adoptive immunotherapy
WO2017194782A2 (en) * 2016-05-13 2017-11-16 Orionis Biosciences Nv Therapeutic targeting of non-cellular structures

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
KASPAR，M，ET.AL.: "Fibronectin as target for tumor therapy", 《INTERNATIONAL JOURNAL OF CANCER》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024040658A1 (zh) * 2022-08-26 2024-02-29 南京融捷康生物科技有限公司 一种抗trem2的单域抗体及其用途

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