CN112201305A - Gene number measuring equipment in single cell nucleus - Google Patents
Gene number measuring equipment in single cell nucleus Download PDFInfo
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- CN112201305A CN112201305A CN202011081537.7A CN202011081537A CN112201305A CN 112201305 A CN112201305 A CN 112201305A CN 202011081537 A CN202011081537 A CN 202011081537A CN 112201305 A CN112201305 A CN 112201305A
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
- G16B20/30—Detection of binding sites or motifs
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Abstract
The invention discloses a device for measuring the number of genes in a single cell nucleus, which comprises a device body, wherein a function cavity is arranged in the device body, a turnover cavity with a rightward opening is arranged at the right side of the function cavity, a turnover rotating shaft is rotatably arranged at the upper side between the front wall and the rear wall of the turnover cavity, and a turnover plate is fixedly connected to the lower side of the excircle end surface of the turnover rotating shaft; according to the invention, the gene culture tanks are arranged and placed, so that the gene culture tanks can perform gene amplification in an environment with uniform temperature, and then when critical fifty-eight degree annealing is performed, the device is rotated and sealed, and then the device is cooled after heat dissipation and ventilation, so that the annealing requirement is met, the sealing device is treated, so that the gene culture environment temperature in the annealing link is more stable, the manual operation steps of experimenters are reduced, intelligent and automatic culture is realized, and the efficiency of subsequent gene number determination is improved.
Description
Technical Field
The invention relates to the technical field of single cell gene number, in particular to a device for measuring the number of genes in a single cell nucleus.
Background
In our daily life, the single cell whole genome side sequencing technology is a new technology for amplifying and sequencing whole genome at single cell level, the principle is that the separated micro whole genome DNA of a single cell is amplified to obtain a complete genome with high coverage rate and then high-throughput sequencing is carried out, the whole gene detection is generally carried out by adopting the MALBAC technology at present, in order to realize the uniform amplification of gene segments and the coverage of the whole genome, four steps of Quencing (0 ℃), Extension (65 ℃), Melting (94 ℃) and Looping (58 ℃) are required, wherein the temperature control influences the gene amplification efficiency, five times of cycles are needed in the culture process, the process is complicated in multiple steps and inconvenient for experimenters to culture, and the device for measuring the number of genes in the single cell nucleus can solve the problems.
Disclosure of Invention
The invention aims to provide a device for measuring the number of genes in a single cell nucleus, and solves the problems of low detection efficiency and low accuracy of a vacuum packaging bag.
The invention is realized by the following technical scheme.
The invention relates to a device for measuring the number of genes in a single cell nucleus, which comprises a device body, wherein a function cavity is arranged in the device body, a turnover cavity with a rightward opening is arranged at the right side of the function cavity, a turnover rotating shaft is rotatably arranged at the upper side between the front wall and the rear wall of the turnover cavity, a turnover plate is fixedly connected to the lower side of the excircle end surface of the turnover rotating shaft, an elastic closed elastic plate is fixedly connected to the left end surface of the turnover plate, a moving box which can move left and right and can rotate is arranged in the function cavity, a culture cavity with a rightward opening is arranged in the moving box, a supporting round rod is fixedly arranged at the left side between the upper wall and the lower wall in the culture cavity in a rotating manner, six storage plates which are arranged in an up-and-down matrix manner are fixedly connected to the excircle end surface of the supporting round rod, culture tanks for placing culture solution are distributed on the upper side in the culture plate, the right side of the lower wall of the function cavity is communicated with a moving cavity with an upward opening, a supporting rotating shaft is rotatably arranged on the right side between the front wall and the rear wall in the moving cavity, the excircle end surface of the supporting rotating shaft is fixedly connected with a supporting rotating wheel, the excircle end surface of the supporting rotating wheel is abutted against the lower end surface of the moving box, a rotating wheel capable of moving left and right is arranged on the left side in the moving cavity, the axle center of the rotating wheel is fixedly connected with a moving rotating shaft, a moving support plate is arranged on the upper side of the rotating wheel, the front end and the rear end of the moving rotating shaft are rotatably connected with the moving support plate, a rotating support plate is fixedly arranged at the upper end of the moving support plate, the upper end surface of the rotating support plate, the utility model discloses a temperature detector, including function chamber, fan chamber, heat dissipation channel, fan chamber, heat dissipation channel, function chamber inner wall, fan chamber, heat dissipation channel, fan chamber, function chamber inner wall back side, the function chamber inner wall back wall is equipped with the removal connecting plate that can the back-and-forth movement, the upper end is equipped with the heat dissipation channel that runs through around being equipped with in the removal connecting plate, the function chamber back wall terminal surface intercommunication is equipped with the sliding spring, the connecting spring is equipped with the link block that can the back-and-forth movement, the link block preceding terminal surface with remove connecting plate rear end fixed connection, the upside is equipped with the lift slider that can reciprocate in the.
Preferably, the pushing cavity is internally provided with a pushing rod capable of moving left and right, the right end face of the pushing rod is fixedly connected with the left end face of the pushing moving plate, the left end face of the pushing cavity is rotatably provided with a pushing screw rod, the pushing screw rod is in threaded connection with the pushing rod, a pushing motor is fixedly arranged in the left wall of the pushing cavity, and the left end of the pushing screw rod is in power connection with the pushing motor.
Preferably, a movable sliding plate capable of moving back and forth is arranged in the sliding propulsion cavity and on the rear side of the connecting slide block, a sliding spring is fixedly connected between the front end face of the movable sliding plate and the rear end face of the connecting slide block, a transmission rotating shaft is rotatably arranged on the rear wall in the sliding propulsion cavity, and the transmission rotating shaft is in threaded connection with the movable sliding plate.
But preferred, terminal surface intercommunication is equipped with annular support rotation chamber under the function chamber, it is equipped with to rotate to remove and convex support electromagnetic plate to support the rotation intracavity, it is equipped with the rotation spout of semicircle sector to support rotation intracavity lower wall intercommunication, support electromagnetic plate lower terminal surface fixedly connected with lower extreme extends to rotate the support cardboard in the spout, support the electromagnetic plate upper end with terminal surface left side is contradicted under the removal case, it is equipped with the rotation spur gear to support to rotate intracavity intermediate position, rotate spur gear excircle terminal surface with support electromagnetic plate right-hand member face gear meshing, it just is located to support to rotate in the chamber lower wall rotate the fixed rotation motor that is equipped with in spur gear below, rotate the spur gear lower extreme with it connects to rotate motor power.
Preferably, a belt wheel cavity is arranged on the lower side of the rotating motor and obliquely behind right, a driving belt wheel is arranged on the left wall of the belt wheel cavity in a rotating mode, the upper end of the driving belt wheel is in power connection with the rotating motor, a driven belt wheel is arranged on the right wall of the belt wheel cavity in a rotating mode, a transmission belt is arranged between the driven belt wheel and the driving belt wheel in a winding mode, a transmission rotating shaft is fixedly connected to the axis of the upper end face of the driven belt wheel, the upper wall of the belt wheel cavity is arranged in the rear wall of the sliding propulsion cavity and provided with a gear cavity, the upper end face of the transmission rotating shaft extends upwards into the gear cavity and is fixedly connected with a rotating bevel gear, a transmission bevel gear meshed with the rotating bevel gear is arranged on the front wall of the gear cavity in a rotating mode.
Preferably, the lifting slide block up end with fixedly connected with lifting spring between the lift chamber upper wall terminal surface, the flexible stay cord of lifting slide block up end fixedly connected with, fan intracavity left side wall intercommunication is equipped with the air pipe of opening left, fan chamber back wall internal fixation is equipped with the energy supply motor, fan rear end power connect in the energy supply motor.
But preferred, energy supply motor rear side is equipped with the centrifugal chamber, the centrifugal chamber internal rotation is equipped with the centrifugal wheel, centrifugal wheel front end power connect in the energy supply motor, the annular matrix arrangement of centrifugal wheel excircle terminal surface is equipped with the outside centrifugal removal chamber of opening, the centrifugal removal intracavity is equipped with the centrifugal slider that can reciprocate, centrifugal slider is close to centrifugal wheel axle center a side end face with the centrifugal removal chamber is close to fixedly connected with centrifugal spring between the centrifugal wheel side end wall, the centrifugal intracavity just is located the centrifugal wheel below is equipped with the separation and reunion push pedal that can reciprocate, terminal surface under the separation and reunion push pedal with fixedly connected with connecting spring between the centrifugal chamber lower wall terminal surface, terminal surface fixedly connected with connects the stay cord under the separation and reunion push pedal, connect the stay cord with flexible stay cord is connected.
The invention has the beneficial effects that: according to the invention, the gene culture tanks are arranged and placed, so that the gene culture tanks can perform gene amplification in an environment with uniform temperature, and then when critical fifty-eight degree annealing is performed, the device is rotated and sealed, and then the device is cooled after heat dissipation and ventilation, so that the annealing requirement is met, the sealing device is treated, so that the gene culture environment temperature in the annealing link is more stable, the manual operation steps of experimenters are reduced, intelligent and automatic culture is realized, and the efficiency of subsequent gene number determination is improved.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the invention, and it is obvious for those skilled in the art that other drawings can be obtained based on these drawings without creative efforts.
FIG. 1 is a schematic structural diagram of an embodiment of the present invention;
3 FIG. 32 3 is 3 a 3 schematic 3 structural 3 diagram 3 of 3 A 3- 3 A 3 in 3 FIG. 31 3 according 3 to 3 an 3 embodiment 3 of 3 the 3 present 3 invention 3; 3
FIG. 3 is a schematic structural diagram of B-B in FIG. 1 according to an embodiment of the present invention;
FIG. 4 is a schematic structural diagram of C-C in FIG. 1 according to an embodiment of the present invention;
FIG. 5 is an enlarged schematic view of the embodiment of the present invention at D in FIG. 1;
FIG. 6 is an enlarged schematic view of the embodiment of the present invention shown in FIG. 2 at E.
Detailed Description
The invention will now be described in detail with reference to fig. 1-6, wherein for ease of description the orientations described hereinafter are now defined as follows: the up, down, left, right, and front-back directions described below correspond to the up, down, left, right, and front-back directions in the projection relationship of fig. 1 itself.
Combine 1-6 a gene figure survey equipment in monosuclear nucleus, including equipment organism 11, be equipped with function chamber 32 in the equipment organism 11, function chamber 32 right side intercommunication is equipped with opening upset chamber 28 to the right, upside rotation is equipped with upset pivot 29 between two walls around the upset chamber 28, upset pivot 29 excircle terminal surface downside fixedly connected with returning face plate 23, returning face plate 23 left end face fixedly connected with has elastic sealed elastic plate 24, be equipped with in the function chamber 32 and remove and can the rotatory position move the case 31, be equipped with opening cultivation chamber 27 to the right in the removal case 31, it is fixed to be equipped with support round bar 30 to rotate on the left side between the upper and lower wall in cultivation chamber 27, support round bar 30 excircle terminal surface fixedly connected with six about the matrix arrangement's of reservoir plate 26, each side upside is equipped with three ascending storage tank 25 of opening in the reservoir plate 26, the culture plate 75 which can be conveniently grabbed is placed in the storage tank 25, culture tanks 56 for placing culture solution are distributed on the upper side in the culture plate 75 in an arrayed manner, the right side of the lower wall of the function cavity 32 is communicated with a moving cavity 21 with an upward opening, a supporting rotating shaft 22 is arranged between the front wall and the rear wall of the moving cavity 21 in a rotating manner, the excircle end surface of the supporting rotating shaft 22 is fixedly connected with a supporting rotating wheel 52, the excircle end surface of the supporting rotating wheel 52 is abutted to the lower end surface of the moving box 31, a rotating wheel 35 capable of moving left and right is arranged on the left side in the moving cavity 21, a moving rotating shaft 34 is fixedly connected with the axis of the rotating wheel 35, a moving support plate 55 is arranged on the upper side of the rotating wheel 35, the front end and the rear end of the moving rotating shaft 34 are rotatably connected with the moving support plate 55, a rotating support plate 57 is fixedly, the end face of the wall of the function cavity 32 is communicated with a pushing cavity 13, a pushing moving plate 12 capable of moving left and right is arranged in the pushing cavity 13, a moving connecting plate 49 capable of moving back and forth is arranged on the inner rear wall of the function cavity 32, a heat dissipation channel 51 penetrating through the moving connecting plate 49 is arranged at the upper end in the moving connecting plate 49 in a front-back manner, a sliding spring 47 is arranged on the end face of the rear wall of the function cavity 32 in a communicating manner, a connecting slider 48 capable of moving back and forth is arranged in the sliding spring 47, the front end face of the connecting slider 48 is fixedly connected with the rear end face of the moving connecting plate 49, a fan cavity 69 is arranged on the upper side in the rear wall of the function cavity 32, a communicating pipeline 58 is arranged between the fan cavity 69 and the function cavity 32 in a communicating manner, the communicating pipeline 58 is communicated with the heat dissipation channel 51, a lifting, a fan 70 is rotatably arranged in the fan cavity 69, and a temperature detector 50 for detecting temperature is fixedly arranged on the rear side of the upper wall of the functional cavity 32.
Beneficially, a pushing rod 15 capable of moving left and right is arranged in the pushing cavity 13, the right end face of the pushing rod 15 is fixedly connected with the left end face of the pushing moving plate 12, a pushing screw 16 is rotatably arranged on the left end face in the pushing cavity 13, the pushing screw 16 is in threaded connection with the pushing rod 15, a pushing motor 14 is fixedly arranged in the left wall of the pushing cavity 13, and the left end of the pushing screw 16 is in power connection with the pushing motor 14.
Advantageously, a movable sliding plate 41 capable of moving back and forth is arranged in the sliding propulsion cavity 46 and behind the connecting slide block 48, a sliding spring 47 is fixedly connected between the front end surface of the movable sliding plate 41 and the rear end surface of the connecting slide block 48, a transmission rotating shaft 42 is rotatably arranged on the rear wall in the sliding propulsion cavity 46, and the transmission rotating shaft 42 is in threaded connection with the movable sliding plate 41.
Beneficially, terminal surface intercommunication is equipped with annular support rotation chamber 38 under the function chamber 32, it can rotate removal and convex support electromagnetic plate 37 to be equipped with in the rotation chamber 38 to support, it is equipped with semi-circular sector's rotation spout 53 to support the lower wall intercommunication in the rotation chamber 38, support electromagnetic plate 37 terminal surface fixedly connected with lower extreme extend to rotate support cardboard 36 in the spout 53, support electromagnetic plate 37 upper end with terminal surface left side is contradicted under the movable box 31, it is equipped with rotation spur gear 54 to support the rotation intracavity 38 internal intermediate position rotation, rotate spur gear 54 excircle terminal surface with support electromagnetic plate 37 right-hand member face gear meshing, it just is located to support in the rotation chamber 38 lower wall rotate the fixed rotation motor 17 that is equipped with in spur gear 54 below, rotate spur gear 54 lower extreme with rotate motor 17 power and connect.
Advantageously, the lower side of the rotating motor 17 is provided with a belt wheel cavity 20 obliquely right and back, the left wall in the belt wheel cavity 20 is rotatably provided with a driving belt wheel 18, the upper end of the driving belt wheel 18 is connected with the rotating motor 17 in a power mode, a driven belt wheel 39 is rotationally arranged on the right wall in the belt wheel cavity 20, a transmission belt 19 is wound between the driven belt wheel 39 and the driving belt wheel 18, a transmission rotating shaft 40 is fixedly connected with the shaft center of the upper end surface of the driven belt wheel 39, a gear cavity 44 is arranged in the upper wall of the belt wheel cavity 20 and positioned in the rear wall of the sliding propulsion cavity 46, the upper end of the transmission shaft 40 extends upwards into the gear cavity 44 and is fixedly connected with a rotation bevel gear 43, the inner front wall of the gear cavity 44 is rotatably provided with a transmission bevel gear 45 engaged with the rotation bevel gear 43, the rear end face of the transmission rotating shaft 42 extends backwards into the gear cavity 44 and is fixedly connected with the axis of the front section face of the transmission bevel gear 45.
Beneficially, lifting spring 73 is fixedly connected between the upper end face of lifting slider 74 and the upper wall end face of lifting cavity 72, telescopic pull rope 71 is fixedly connected to the upper end face of lifting slider 74, ventilation duct 59 with a leftward opening is communicated with the left wall in fan cavity 69, energy supply motor 60 is fixedly arranged in the rear wall of fan cavity 69, and the rear end of fan 70 is dynamically connected to energy supply motor 60.
Advantageously, a centrifugal cavity 68 is arranged at the rear side of the energy supply motor 60, a centrifugal wheel 65 is rotatably arranged in the centrifugal cavity 68, the front end of the centrifugal wheel 65 is connected with the energy supply motor 60 in a power mode, the outer circle end face of the centrifugal wheel 65 is provided with centrifugal moving cavities 66 with outward openings in an annular matrix arrangement, a centrifugal slide block 67 capable of moving up and down is arranged in the centrifugal moving cavity 66, a centrifugal spring 64 is fixedly connected between the end surface of one side of the centrifugal slide block 67 close to the axis of the centrifugal wheel 65 and the end wall of one side of the centrifugal moving cavity 66 close to the centrifugal wheel 65, a clutch push plate 63 which can move up and down is arranged in the centrifugal cavity 68 and below the centrifugal wheel 65, a connecting spring 62 is fixedly connected between the lower end surface of the clutch push plate 63 and the end surface of the lower wall of the centrifugal cavity 68, the lower end face of the clutch push plate 63 is fixedly connected with a connecting pull rope 61, and the connecting pull rope 61 is connected with the telescopic pull rope 71.
In the initial state, the pushing moving plate 12 is located at the left limit position, the heat dissipation channel 51 is located at the rear limit position, the clutch pushing plate 63 is located at the upper limit position, and the lifting slider 74 is located at the lower limit position.
When the device works, the pushing motor 14 is started to drive the pushing screw 16 to rotate, the pushing screw 16 rotates to drive the pushing rod 15 to move rightwards through threaded connection, at the moment, the pushing rod 15 moves rightwards to drive the pushing moving plate 12 to move rightwards and abut against the left end face of the moving box 31, the moving box 31 is pushed to move rightwards, at the moment, the moving box 31 slides through the supporting rotating wheel 52 and the moving rotating shaft 34 to reduce resistance and simultaneously facilitates the moving box 31 to move rightwards, at the moment, the right end face of the moving box 31 abuts against the closed elastic plate 24 on the left end face of the turnover plate 23 and pushes the turnover plate 23 to turn over and open, when the pushing moving plate 12 moves to the right limit position, the pushing motor 14 rotates reversely to drive the pushing moving plate 12 to move back to the initial working position, at the moment, an experimenter places each culture plate 75, at the moment, the turnover plate 23 is turned inwards and closed, and a good sealing effect is achieved due to the elasticity of the sealing elastic plate 24, at the moment, the inside of the movable box 31 is subjected to a temperature change for culture, when a key link of annealing is carried out, the rotating motor 17 is started to drive the rotating spur gear 54 to rotate, the rotating spur gear 54 drives the supporting electromagnetic plate 37 to rotate through gear meshing, at the moment, the supporting electromagnetic plate 37 is clamped in the rotating chute 53 through the supporting clamping plate 36, the supporting electromagnetic plate 37 rotates and moves along the direction of the rotating chute 53, at the same time, the supporting electromagnetic plate 37 is started to be electrified and has magnetism, at the moment, the movable box 31 is driven to rotate in the moving process of the supporting electromagnetic plate 37, at the same time, the rotating motor 17 drives the driving pulley 18 to rotate, the driving pulley 18 drives the driven pulley 39 to rotate through the driving belt 19, the driven pulley 39 drives the rotating, the rotating bevel gear 43 drives the driving bevel gear 45 to rotate through gear engagement, the driving bevel gear 45 drives the driving rotating shaft 42 to rotate, at the moment, the driving rotating shaft 42 drives the moving sliding plate 41 to move forwards through threaded connection, at the moment, the moving sliding plate 41 moves forwards to push the connecting slide block 48 to move forwards through the elastic force action of the sliding spring 47 and drive the moving connecting plate 49 to move forwards, the effect of gradually closing the opening of the moving box 31 in the rotating direction process of the moving box 31 is achieved, at the moment, the moving connecting plate 49 is closed with the moving box 31, at the moment, the temperature is detected through the temperature detector 50, the energy supply motor 60 is started to drive the fan 70 to rotate, the fan 70 grabs and exhausts air in the fan cavity 69 through the ventilation pipeline 59 to the outside of the device, at the same time, the energy supply motor 60 drives the centrifugal wheel 65 to rotate, the centrifugal wheel 65 rotates to enable the centrifugal slide block 67 to be under The plate 63 overcomes the elastic force of the connecting spring 62 to move downwards, at the moment, the clutch push plate 63 moves downwards to enable the connecting pull rope 61 to be connected with the telescopic pull rope 71 without tensioning, at the moment, the lifting slide block 74 moves downwards due to the elastic force of the lifting spring 73 and enables the left side and the right side of the communication pipeline 58 to be communicated, at the moment, the communication pipeline 58 is communicated with the heat dissipation channel 51 to discharge internal hot air to achieve the effect of cooling, when the temperature detector 50 detects that the temperature meets the annealing temperature requirement, the energy supply motor 60 stops and seals the left side and the right side of the communication pipeline 58, the purpose that gene culture can be instantly annealed in a sealed space with a proper temperature requirement is achieved, then, after five times of repeated circulation, the device culture is completed, and after the temperature in the equipment body 11 is reduced through the restart of the energy supply motor 60, expe.
The above embodiments are merely illustrative of the technical ideas and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and not to limit the protection scope of the present invention. All equivalent changes and modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.
Claims (7)
1. A gene number measuring device in a single cell nucleus comprises a device body, and is characterized in that: the device is characterized in that a function cavity is arranged in the device body, a turnover cavity with a right opening is arranged on the right side of the function cavity, a turnover rotating shaft is arranged on the upper side between the front wall and the rear wall of the turnover cavity in a rotating manner, a turnover plate is fixedly connected to the lower side of the excircle end surface of the turnover rotating shaft, an elastic closed elastic plate is fixedly connected to the left end surface of the turnover plate, a moving box which can move left and right and can rotate is arranged in the function cavity, a culture cavity with a right opening is arranged in the moving box, a supporting round rod is fixedly arranged between the upper wall and the lower wall in the culture cavity in a rotating manner, six storage plates arranged in an upper matrix and a lower matrix are fixedly connected to the excircle end surface of the supporting round rod, three storage grooves with upward openings are arranged on the upper side in the storage plates, culture plates which can, the right side of the lower wall of the functional cavity is communicated with a moving cavity with an upward opening, a supporting rotating shaft is rotatably arranged on the right side between the front wall and the rear wall in the moving cavity, the excircle end surface of the supporting rotating shaft is fixedly connected with a supporting rotating wheel, the excircle end surface of the supporting rotating wheel is abutted against the lower end surface of the moving box, the left side in the moving cavity is provided with a rotating wheel capable of moving left and right, the axis of the rotating wheel is fixedly connected with a moving rotating shaft, the upper side of the rotating wheel is provided with a moving support plate, the front end and the rear end of the moving rotating shaft are rotatably connected with the moving support plate, the upper end surface of the rotating support plate is rotatably connected with the lower end surface of the moving box, the end surface of the functional cavity is communicated with a pushing cavity, the pushing cavity is internally, the improved temperature detector is characterized in that a heat dissipation channel which runs through the front and the back is arranged at the upper end in the movable connecting plate, a sliding spring is communicated with the end face of the rear wall of the function cavity, a connecting slide block capable of moving back and forth is arranged in the sliding spring, the front end face of the connecting slide block is fixedly connected with the rear end face of the movable connecting plate, a fan cavity is arranged at the upper side in the rear wall of the function cavity, a communicating pipeline is communicated with the function cavity, the communicating pipeline is communicated with the heat dissipation channel, a lifting cavity is communicated with the upper wall and the lower wall of the communicating pipeline, a lifting slide block capable of moving up and down is arranged in the lifting cavity, a fan is arranged in the fan cavity in a rotating mode, and a.
2. The apparatus for determining the number of genes in a single cell nucleus according to claim 1, wherein: the utility model discloses a push rod, including promotion intracavity, push rod, propelling movement board.
3. The apparatus for determining the number of genes in a single cell nucleus according to claim 1, wherein: the sliding pushing cavity is internally provided with a connecting slide block, the rear side of the connecting slide block is provided with a movable sliding plate capable of moving back and forth, a sliding spring is fixedly connected between the front end surface of the movable sliding plate and the rear end surface of the connecting slide block, the rear wall in the sliding pushing cavity is rotatably provided with a transmission rotating shaft, and the transmission rotating shaft is in threaded connection with the movable sliding plate.
4. The apparatus for determining the number of genes in a single cell nucleus according to claim 1, wherein: terminal surface intercommunication is equipped with annular support rotation chamber under the function chamber, it is equipped with to rotate to remove and convex support electromagnetic plate to support to rotate the intracavity, it is equipped with the rotation spout of semicircle sector to support rotation intracavity lower wall intercommunication, support electromagnetic plate lower terminal surface fixedly connected with lower extreme extend to rotate the support cardboard in the spout, support the electromagnetic plate upper end with terminal surface left side is contradicted under the removal case, it rotates intracavity intermediate position to support to rotate and is equipped with and rotates the spur gear, rotate straight-gear excircle terminal surface with support electromagnetic plate right-hand member face gear meshing, it just is located to support to rotate in the chamber lower wall rotate the fixed rotation motor that is equipped with in straight-gear below, rotate straight-gear lower extreme with it connects to rotate motor power.
5. The apparatus for determining the number of genes in a single cell nucleus according to claim 4, wherein: the utility model discloses a slip propulsion device, including rotation motor, drive pulley, driven pulley, drive pulley, slip propulsion chamber, transmission shaft, drive pulley upper end power connect in rotate the motor, the right wall of band pulley intracavity rotates and is equipped with driven pulley, driven pulley with around being equipped with driving belt between the drive pulley, driven pulley up end axle center fixedly connected with transmission pivot, just be located in the band pulley chamber upper wall be equipped with the gear chamber in the slip propulsion chamber back wall, transmission pivot up end upwards extends to gear intracavity and fixedly connected with rotate bevel gear, the antetheca rotates and is equipped with rotate bevel gear engaged with, transmission pivot rear end surface backwards extends to gear intracavity and fixed connection in transmission bevel gear front end section axle center face.
6. The apparatus for determining the number of genes in a single cell nucleus according to claim 1, wherein: lifting slide up end with fixedly connected with lifting spring between the lift chamber upper wall terminal surface, the flexible stay cord of lifting slide up end fixedly connected with, fan intracavity left side wall intercommunication is equipped with the air pipe of opening left, fan chamber back wall internal fixation is equipped with the energy supply motor, fan rear end power connect in the energy supply motor.
7. The apparatus for determining the number of genes in a single cell nucleus according to claim 6, wherein: energy supply motor rear side is equipped with the centrifugal chamber, the centrifugal intracavity rotation is equipped with the centrifugal wheel, centrifugal wheel front end power connect in the energy supply motor, the annular matrix arrangement of centrifugal wheel excircle terminal surface is equipped with the outside centrifugal removal chamber of opening, the centrifugation removes the intracavity and is equipped with the centrifugal slider that can reciprocate, centrifugal slider is close to centrifugal wheel axle center a side end face with the centrifugation removes the chamber and is close to fixedly connected with centrifugal spring between the centrifugal wheel side end wall, the centrifugal intracavity just is located the centrifugal wheel below is equipped with the separation and reunion push pedal that can reciprocate, terminal surface under the separation and reunion push pedal with fixedly connected with connecting spring between the centrifugal chamber lower wall terminal surface, terminal surface fixedly connected with connects the stay cord under the separation and reunion push pedal, connect the stay cord with flexible stay cord is connected.
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| CN202011081537.7A CN112201305B (en) | 2020-10-12 | 2020-10-12 | Gene number measuring equipment in single cell nucleus |
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| CN202011081537.7A CN112201305B (en) | 2020-10-12 | 2020-10-12 | Gene number measuring equipment in single cell nucleus |
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| CN112201305B CN112201305B (en) | 2021-12-07 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113441201A (en) * | 2021-08-16 | 2021-09-28 | 谢梦池 | Clinical laboratory's sample storage device |
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