CN112198028A - Sample pretreatment device for microbial soil detection - Google Patents

Sample pretreatment device for microbial soil detection Download PDF

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Publication number
CN112198028A
CN112198028A CN202011096814.1A CN202011096814A CN112198028A CN 112198028 A CN112198028 A CN 112198028A CN 202011096814 A CN202011096814 A CN 202011096814A CN 112198028 A CN112198028 A CN 112198028A
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cavity
stirring
soil
box body
heating
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CN202011096814.1A
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Chinese (zh)
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金善军
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Individual
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Priority to CN202011096814.1A priority Critical patent/CN112198028A/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/34Purifying; Cleaning
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/44Sample treatment involving radiation, e.g. heat

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Processing Of Solid Wastes (AREA)

Abstract

The invention discloses a sample pretreatment device for microbial soil detection, which comprises a box body, wherein a stirring cavity is arranged in the box body, a heating cavity, a compression cavity and a rotating cavity are sequentially arranged in the box body from left to right, the heating cavity, the compression cavity and the rotating cavity are all positioned above the stirring cavity, a collecting groove and a trigger mechanism are arranged at the inner bottom of the stirring cavity, the trigger mechanism comprises a first rotating rod vertically arranged in the rotating cavity, a motor is arranged at the upper end of the box body, and the tail end of an output shaft of the motor extends into the rotating cavity. The device stirs soil through a plurality of stirring rods for microorganism soil detection device detects soil more easily, reduces the possibility of detection error, still lets in hot-blastly in to soil, reduces the moisture in the soil, improves the garrulous effect of stirring, and filters debris through the vibrations of otter board, makes not have debris in the soil that is detected, lets detect more accurately.

Description

Sample pretreatment device for microbial soil detection
Technical Field
The invention relates to the field of microbial soil detection, in particular to a sample pretreatment device for microbial soil detection.
Background
The soil microorganisms are of various types, and to perform scientific research on the microorganisms or to detect the microorganisms in the soil, the first condition is to obtain a suitable soil sample, and generally, when obtaining a suitable soil sample, the following problems are encountered in the process of pretreating the soil sample and then pretreating the soil sample with the microorganisms:
when traditional microorganism soil detection device carries out the preliminary treatment to the soil that needs detected, it is general exactly to stir garrulous soil, then filter wherein great impurity, but moisture is more in some soil, the stickness is great, make soil be difficult to by the garrulous, directly carry out subsequent filtration, produced filter effect is relatively poor, and simultaneously, traditional preprocessing device, when screening the filtration to the soil after the garrulous, need use vibrating motor, shake the filter screen, it is extravagant to have caused the unnecessary energy.
Disclosure of Invention
The invention aims to solve the defects in the prior art, and provides a sample pretreatment device for microbial soil detection.
In order to achieve the purpose, the invention adopts the following technical scheme:
a sample pretreatment device for microbial soil detection comprises a box body, wherein a stirring cavity is arranged in the box body, a heating cavity, a compression cavity and a rotation cavity are sequentially arranged in the box body from left to right, the heating cavity, the compression cavity and the rotation cavity are all positioned above the stirring cavity, and a collecting tank is arranged at the inner bottom of the stirring cavity; the trigger mechanism comprises a first rotating rod vertically arranged in a rotating cavity, a motor is installed at the upper end of the box body, the tail end of an output shaft of the motor extends into the rotating cavity and is fixedly connected with the upper end of the first rotating rod, the lower end of the first rotating rod extends into the stirring cavity, and a magnetic sleeve is fixedly connected to the part, located in the rotating cavity, of the first rotating rod; the stirring mechanism comprises a second rotating rod vertically arranged in a stirring cavity, the upper end of the second rotating rod is rotatably connected with the inner top of the stirring cavity, a second gear is fixedly connected to the second rotating rod, the first rotating rod is located on a first gear of the part fixedly connected with of the stirring cavity, the first gear is meshed with the second gear, and a plurality of stirring rods are fixedly connected to the outer sides of the first rotating rod and the second rotating rod.
Preferably, still include heating mechanism, heating mechanism includes the vertical magnetism slide that sets up in the compression chamber, the inner wall sliding connection of magnetism slide and compression chamber, magnetism slide cooperatees with the magnetic sleeve, the left end of magnetism slide and the inner wall in compression chamber are through first spring elastic connection, the left side space and the external world in compression chamber pass through the intake pipe intercommunication, the left side space and the heating chamber in compression chamber pass through the outlet duct intercommunication, the heating wire is installed to the interior bottom in heating chamber, the hollow plate of inner wall fixedly connected with arc in stirring chamber, a plurality of bleeder vents have been seted up on the hollow plate inside wall of arc, the hollow plate of arc passes through the breather pipe intercommunication with the heating chamber.
Preferably, all install the check valve on intake pipe and the outlet duct, set up the vent with external intercommunication on the right side inner wall in stirring chamber, the stirring intracavity level is equipped with the otter board, the otter board is connected with the inner wall fixed connection in stirring chamber, the front side of box is installed and is pulled the door.
Preferably, all be equipped with the shifting chute on the left and right sides inner wall in stirring chamber, both ends all run through the notch of corresponding shifting chute about the otter board to extend to in the shifting chute, the otter board all passes through second spring elastic connection with the interior top of two shifting chutes, the otter board all is connected through folding gasbag with the interior bottom of two shifting chutes, two folding gasbag passes through communicating pipe intercommunication, the right side space and the communicating pipe of compression chamber pass through the connecting pipe intercommunication.
Compared with the prior art, the invention has the beneficial effects that:
1. be provided with the stirring mechanism, drive first bull stick through the motor and rotate, first bull stick drives first gear revolve, first gear drive rather than the second gear rotation of meshing, the second gear drives the second bull stick and rotates, first bull stick and second bull stick all drive a plurality of stirring rods and stir garrulous, the going on of the follow-up filtering process of being convenient for to soil.
2. Be provided with heating mechanism, remove through the removal about the magnetism slide, when the magnetism slide moves to the right, the left side space in compression chamber passes through the intake pipe and inhales gas from the external world, when the magnetism slide moves to the left, gas in the left side space in compression chamber passes through the intake pipe and gets into the heating intracavity, the electric heat wire is to the air heating of heating intracavity, because the atmospheric pressure effect, make the steam of heating intracavity pass through the breather pipe and get into the hollow inboard of arc, and spout to soil by a plurality of air vents, reduce the moisture in the soil, improve the effect of stirring garrulous, soil that the stirring is garrulous passes through the otter board more easily, and filter great impurity in the soil.
3. Be provided with shake mechanism, remove through the removal about the magnetism slide, drive the otter board and reciprocate, filter debris through the vibrations of otter board for the debris content of the soil that is detected diminishes, and does not need vibrating motor to drive the otter board vibrations and filters debris, reduces the waste of the energy.
Drawings
FIG. 1 is a schematic structural diagram of a sample pretreatment device for microbial soil detection according to the present invention;
FIG. 2 is a schematic front view of the structure of FIG. 1;
FIG. 3 is a schematic structural diagram of embodiment 2 of the present invention;
fig. 4 is an enlarged view of fig. 3 at a.
In the figure: the device comprises a box body 1, a rotating cavity 2, a compression cavity 3, a heating cavity 4, a stirring cavity 5, a collecting tank 6, a motor 7, a first rotating rod 8, a magnetic sleeve 9, a magnetic sliding plate 10, a first spring 11, an air inlet pipe 12, an air outlet pipe 13, an electric heating wire 14, a vent pipe 15, a hollow arc plate 16, a vent hole 17, a first gear 18, a second gear 19, a second rotating rod 20, a stirring rod 21, a net plate 22, a connecting pipe 23, a communicating pipe 24, a moving groove 25, a second spring 26, a folding air bag 27 and a sliding door 28.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
Example 1
Referring to fig. 1-2, a sample pretreatment device for microbial soil detection comprises a box body 1, wherein a stirring cavity 5 is arranged in the box body 1, a heating cavity 4, a compression cavity 3 and a rotation cavity 2 are sequentially arranged in the box body 1 from left to right, the heating cavity 4, the compression cavity 3 and the rotation cavity 2 are all positioned above the stirring cavity 5, and a collecting tank 6 is arranged at the inner bottom of the stirring cavity 5;
the trigger mechanism comprises a first rotating rod 8 vertically arranged in the rotating cavity 2, a motor 7 is installed at the upper end of the box body 1, the tail end of an output shaft of the motor 7 extends into the rotating cavity 2 and is fixedly connected with the upper end of the first rotating rod 8, the lower end of the first rotating rod 8 extends into the stirring cavity 5, and a magnetic sleeve 9 is fixedly connected to the part, located in the rotating cavity 2, of the first rotating rod 8;
stirring mechanism, stirring mechanism includes vertical setting second bull stick 20 in stirring chamber 5, the upper end of second bull stick 20 rotates with the interior top of stirring chamber 5 to be connected, fixedly connected with second gear 19 on the second bull stick 20, first bull stick 8 is located the first gear 18 of part fixedly connected with of stirring chamber 5, first gear 18 and the meshing of second gear 19 mutually, a plurality of stirring rods 21 of outside fixedly connected with of first bull stick 8 and second bull stick 20, stir the bits of broken glass with soil through stirring rod 21, make microorganism soil detection device detect soil more easily, reduce the possibility of detection error.
Wherein, the heating mechanism comprises a magnetic sliding plate 10 vertically arranged in the compression cavity 3, the magnetic sliding plate 10 is connected with the inner wall of the compression cavity 3 in a sliding way, the magnetic sliding plate 10 is matched with a magnetic sleeve 9, the left side of the magnetic sliding plate 10 is an S pole, the right side of the magnetic sliding plate 10 is an N pole, the magnetic sleeve 9 consists of two semi-arc magnetic rings, when the S pole of the magnetic sleeve 9 is positioned on the left side, the magnetic sliding plate 10 and the magnetic sleeve 9 attract each other in an opposite polarity manner, so that the magnetic sliding plate 10 moves to the right side, when the magnetic sleeve 9 rotates to ensure that the N pole is positioned on the left side, the magnetic sliding plate 10 and the magnetic sleeve 9 repel each other in an equal polarity manner, the left end of the magnetic sliding plate 10 is elastically connected with the inner wall of the compression cavity 3 through a first spring 11, the space on the left side of the compression cavity 3 is communicated with the outside through an air inlet pipe 12, the hollow board 16 of inner wall fixedly connected with arc of stirring chamber 5 has seted up a plurality of bleeder vents 17 on the hollow board 16 inside wall of arc, and the hollow board 16 of arc communicates through breather pipe 15 with heating chamber 4, through letting in hot-blast in to soil, reduces the moisture in the soil, improves and stirs garrulous effect.
The air inlet pipe 12 and the air outlet pipe 13 are both provided with one-way valves, the one-way valves can ensure that the air inlet pipe 12 can inlet air and ensure that the air outlet pipe 13 can outlet air, the inner wall of the right side of the stirring cavity 5 is provided with a vent communicated with the outside, the vent can discharge the air in the stirring cavity 5, a screen plate 22 is horizontally arranged in the stirring cavity 5, the screen plate 22 is fixedly connected with the inner wall of the stirring cavity 5, the front side of the box body 1 is provided with a sliding door 28, and the sliding door 28 is opened to take out the soil;
wherein, the motor 7 and the heating wire 14 are both electrically connected with an external power supply.
When the microorganism detection is needed to be carried out on soil, firstly, the sliding door 28 is opened, the soil is placed on the screen plate 22, the sliding door 28 is closed, the motor 7 is opened, the motor 7 drives the first rotating rod 8 to rotate, the first rotating rod 8 drives the first gear 18 to rotate, the first gear 18 drives the second gear 19 meshed with the first gear to rotate, the second gear 19 drives the second rotating rod 20 to rotate, and the first rotating rod 8 and the second rotating rod 20 both drive the stirring rods 21 to stir the soil, so that the subsequent filtering process is facilitated;
when the first rotating rod 8 rotates, the first rotating rod 8 drives the magnetic sleeve 9 to rotate, when the S pole of the magnetic sleeve 9 is positioned on the left side, the magnetic sliding plate 10 and the magnetic sleeve 9 attract each other in opposite polarity, so that the magnetic sliding plate 10 moves to the right, when the magnetic sleeve 9 rotates, so that the N pole is positioned on the left side, the magnetic sliding plate 10 and the magnetic sleeve 9 repel each other in same polarity, so that the magnetic sliding plate 10 moves to the left, namely, when the first rotating rod 8 rotates, the magnetic sliding plate 10 moves to the left and right, when the magnetic sliding plate 10 moves to the right, the left space of the compression cavity 3 adsorbs gas from the outside through the air inlet pipe 12, when the magnetic sliding plate 10 moves to the left, the gas in the left space of the compression cavity 3 enters the heating cavity 4 through the air outlet pipe 13, the electric heating wire 14 heats the air in the heating cavity 4, due to the air pressure, the hot gas in the heating cavity 4 enters the arc-shaped hollow plate, the hot gas is fully contacted with the soil, the moisture in the soil is reduced, the crushing effect is improved, and the crushed soil falls into the collecting tank 6 through the screen plate 22 to be collected;
it is worth mentioning that the heating wire 14 power of this device is lower, and the exhaust steam can not lead to the microorganism death to still through the moisture that reduces in the soil, restrain the activity of microorganism in the soil, make the testing result more accurate, and the motor 7 of this device is adjustable speed motor 7, and in the use, the rotational speed of motor 7 need not be too fast, makes the magnetic sliding plate 10 remove about with the slew rate of guaranteeing magnetic sleeve 9.
Example 2
Referring to fig. 3-4, the present embodiment is different from embodiment 1 in that the inner walls of the left and right sides of the stirring chamber 5 are respectively provided with a moving tank 25, the left and right ends of the mesh plate 22 respectively penetrate through the notches of the corresponding moving tanks 25 and extend into the moving tanks 25, the mesh plate 22 is elastically connected with the inner tops of the two moving tanks 25 through a second spring 26, the upper end of the mesh plate 22 is provided with two baffles, the two baffles are respectively located at the notches of the two moving tanks 25, the mesh plate 22 is connected with the inner bottoms of the two moving tanks 25 through folded air bags 27, the two folded air bags 27 are communicated through a communicating pipe 24, the right space of the compression chamber 3 is communicated with the communicating pipe 24 through a connecting pipe 23, impurities are filtered through vibration of the mesh plate 22, so that the content of impurities in the detected soil is reduced, and in the filtering process, the vibration motor is not required, and the waste of unnecessary energy is reduced.
In this embodiment, when the magnetic sliding plate 10 moves left, make two folding gasbags 27 all shrink through connecting pipe 23 and communicating pipe 24, under the elastic action of second spring 26, drive otter board 22 and move down, when the magnetic sliding plate 10 moves right, make two folding gasbags 27 all expand, make otter board 22 move up, that is to say when the magnetic sliding plate 10 moves left and right, can drive otter board 22 and reciprocate, filter debris through the vibrations of otter board 22, make the debris content of the soil that is detected diminish, and at filterable in-process, do not need vibrating motor to drive otter board 22 vibrations and filter debris, reduce the waste of unnecessary energy.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (4)

1. A sample preprocessing device for microbial soil detection is characterized by comprising:
the box body (1), a stirring cavity (5) is arranged in the box body (1), a heating cavity (4), a compression cavity (3) and a rotating cavity (2) are sequentially arranged in the box body (1) from left to right, the heating cavity (4), the compression cavity (3) and the rotating cavity (2) are all located above the stirring cavity (5), and a collecting tank (6) is arranged at the inner bottom of the stirring cavity (5);
the device comprises a trigger mechanism, wherein the trigger mechanism comprises a first rotating rod (8) vertically arranged in a rotating cavity (2), a motor (7) is installed at the upper end of a box body (1), the tail end of an output shaft of the motor (7) extends into the rotating cavity (2) and is fixedly connected with the upper end of the first rotating rod (8), the lower end of the first rotating rod (8) extends into a stirring cavity (5), and a magnetic sleeve (9) is fixedly connected to the part, located in the rotating cavity (2), of the first rotating rod (8);
stirring mechanism, stirring mechanism includes vertical second bull stick (20) of setting in stirring chamber (5), the upper end of second bull stick (20) is rotated with the interior top in stirring chamber (5) and is connected, fixedly connected with second gear (19) on second bull stick (20), first bull stick (8) are located the first gear of part fixedly connected with (18) of stirring chamber (5), first gear (18) and second gear (19) mesh mutually, a plurality of stirring rods (21) of outside fixedly connected with of first bull stick (8) and second bull stick (20).
2. The sample pretreatment device for microbial soil detection according to claim 1, further comprising a heating mechanism, wherein the heating mechanism comprises a magnetic sliding plate (10) vertically arranged in the compression chamber (3), the magnetic sliding plate (10) is slidably connected with the inner wall of the compression chamber (3), the magnetic sliding plate (10) is matched with the magnetic sleeve (9), the left end of the magnetic sliding plate (10) is elastically connected with the inner wall of the compression chamber (3) through a first spring (11), the left space of the compression chamber (3) is communicated with the outside through an air inlet pipe (12), the left space of the compression chamber (3) is communicated with the heating chamber (4) through an air outlet pipe (13), an electric heating wire (14) is installed at the inner bottom of the heating chamber (4), and an arc hollow plate (16) is fixedly connected with the inner wall of the stirring chamber (5), a plurality of air holes (17) are formed in the inner side wall of the arc-shaped hollow plate (16), and the arc-shaped hollow plate (16) is communicated with the heating cavity (4) through an air pipe (15).
3. The sample pretreatment device for microbial soil detection according to claim 1, wherein the air inlet pipe (12) and the air outlet pipe (13) are both provided with one-way valves, the inner wall of the right side of the stirring cavity (5) is provided with a vent communicated with the outside, a screen plate (22) is horizontally arranged in the stirring cavity (5), the screen plate (22) is fixedly connected with the inner wall of the stirring cavity (5), and the front side of the box body (1) is provided with a sliding door (28).
4. The sample pretreatment device for microbial soil detection according to claim 1, wherein moving grooves (25) are respectively formed in the inner walls of the left side and the right side of the stirring chamber (5), the left end and the right end of the screen plate (22) respectively penetrate through the notches of the corresponding moving grooves (25) and extend into the moving grooves (25), the inner tops of the screen plate (22) and the two moving grooves (25) are elastically connected through a second spring (26), the inner bottoms of the screen plate (22) and the two moving grooves (25) are respectively connected through folding air bags (27), the two folding air bags (27) are communicated through communicating pipes (24), and the right space of the compression chamber (3) is communicated with the communicating pipes (24) through connecting pipes (23).
CN202011096814.1A 2020-10-14 2020-10-14 Sample pretreatment device for microbial soil detection Pending CN112198028A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202011096814.1A CN112198028A (en) 2020-10-14 2020-10-14 Sample pretreatment device for microbial soil detection

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Application Number Priority Date Filing Date Title
CN202011096814.1A CN112198028A (en) 2020-10-14 2020-10-14 Sample pretreatment device for microbial soil detection

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CN112198028A true CN112198028A (en) 2021-01-08

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CN202011096814.1A Pending CN112198028A (en) 2020-10-14 2020-10-14 Sample pretreatment device for microbial soil detection

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113484481A (en) * 2021-08-17 2021-10-08 丽江市质量技术监督综合检测中心 Device for detecting fat content in bean product convenient for sampling
CN115282657A (en) * 2022-08-10 2022-11-04 包能董 Pomace filter equipment is used in fruit juice beverage production
CN117760808A (en) * 2023-12-22 2024-03-26 王丹 Sample drying and screening system and screening method for soil detection

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113484481A (en) * 2021-08-17 2021-10-08 丽江市质量技术监督综合检测中心 Device for detecting fat content in bean product convenient for sampling
CN113484481B (en) * 2021-08-17 2023-04-25 丽江市质量技术监督综合检测中心 Bean product fat content detection device convenient to sample
CN115282657A (en) * 2022-08-10 2022-11-04 包能董 Pomace filter equipment is used in fruit juice beverage production
CN117760808A (en) * 2023-12-22 2024-03-26 王丹 Sample drying and screening system and screening method for soil detection

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