CN112193625A - Pathological specimen preservation method - Google Patents
Pathological specimen preservation method Download PDFInfo
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- CN112193625A CN112193625A CN202011050863.1A CN202011050863A CN112193625A CN 112193625 A CN112193625 A CN 112193625A CN 202011050863 A CN202011050863 A CN 202011050863A CN 112193625 A CN112193625 A CN 112193625A
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- pathological specimen
- aqueous solution
- glassware
- thermostat
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/18—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient
- B65D81/20—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient under vacuum or superatmospheric pressure, or in a special atmosphere, e.g. of inert gas
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/18—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient
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- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Sampling And Sample Adjustment (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a pathological specimen preservation method, which particularly relates to the technical field of medical treatment and specifically comprises the following steps: step one, sampling a pathological specimen; fixing a pathological specimen; step three, storing at normal temperature; step four, filling a paraformaldehyde aqueous solution; and step five, storing the labels in a vacuum environment of the incubator. According to the invention, the glassware filled with pathological specimen tissues is placed in the thermostat, the interior of the thermostat is intelligently regulated and controlled within a normal temperature range, the paraformaldehyde aqueous solution container box with the concentration of 4% is fixed above the glassware in the thermostat, and the delayed opening time of the liquid electromagnetic valve is intelligently controlled by adopting the time relay, so that the paraformaldehyde aqueous solution is supplemented to the interior of the glassware, the condition that the subsequent storage is influenced by the oxidation of the paraformaldehyde aqueous solution in the glassware in the thermostat in the storage process is avoided, meanwhile, the oxygen content around the thermostat can be reduced, and the storage time of the pathological specimen is effectively prolonged.
Description
Technical Field
The invention belongs to the technical field of medical treatment, and particularly relates to a pathological specimen preservation method.
Background
As is well known, the traditional pathological specimen dehydration needs about two days, the period is long, the pollution is large, along with the development of pathological technology, a dehydrator is invented, the dehydration period is reduced, at present, about 16 hours are generally used, materials are taken in the afternoon under general conditions, the specimen is taken out from the dehydrator in the next morning and embedded, the machine runs in the whole night, no one manages, the problems of dryness, hardness, dehydration impermeability and the like exist in the machine when the machine is suddenly powered off and the like are sometimes encountered, the large specimen can be prepared by taking materials for the second time, the tissue of the small specimen is very small, particularly, biopsy specimens such as gastroscopes and the like are obtained again, the pain and psychological burden of a patient are increased, the patient is very reluctant, and medical disputes are easily caused, so that a.
Chinese patent publication No. CN106840813A discloses a method for preserving medical pathological specimens, which comprises wrapping human pathological specimens with filter paper, placing in a plastic embedding box, fixing in formaldehyde solution, heating the embedding box with pathological specimens in alcohol in a heating box, heating in xylene in a heating box, heating in molten paraffin in a heating box, taking out the pathological specimens from the paraffin, embedding conventionally, freezing the pathological specimens, slicing the frozen pathological specimens into 4-5 μm slices, baking the slices, HE-staining the slices, and sealing with polyacrylamide gel, and is suitable for rapid treatment of small pathological specimens to reduce pathological diagnosis period, the waiting time of the patient is reduced, the method is economical and applicable, the operation is simple, toxic and harmful substances are not contained, the method is green and environment-friendly, and the burden of the environment is lightened.
In conclusion, the existing preservation method for pathological specimens is not good, and the use effect is easily influenced by oxidation after the fixing solution formaldehyde aqueous solution is used for a long time.
Disclosure of Invention
The invention provides a pathological specimen preservation method, which is characterized in that a glassware filled with pathological specimen tissues is placed in a thermostat, the interior of the thermostat is intelligently regulated within a normal temperature range, a liquid electromagnetic valve is intelligently controlled by a time relay to supplement paraformaldehyde aqueous solution to the interior of the glassware, the condition that the subsequent preservation is influenced by the oxidation of the paraformaldehyde aqueous solution in the glassware in the thermostat in the preservation process is avoided, meanwhile, the oxygen content around the thermostat can be reduced, and the preservation time of the pathological specimen is effectively prolonged.
The invention is realized in such a way, and provides the following technical scheme: a pathological specimen preservation method specifically comprises the following steps:
sampling a pathological specimen, namely immediately placing the pathological specimen in a glass vessel after the tissue of the pathological specimen is excised and isolated, and preparing to carry out fixation treatment by using a fixing solution;
fixing a pathological specimen, namely pouring a 4% paraformaldehyde aqueous solution into a glass vessel, and controlling the amount of the paraformaldehyde aqueous solution to be more than five times of the amount of the pathological specimen tissue;
step three, preserving at normal temperature, namely placing the glassware filled with pathological specimen tissues in a constant temperature box, and intelligently regulating and controlling the interior of the constant temperature box within a normal temperature range;
filling a paraformaldehyde aqueous solution, namely fixing a paraformaldehyde aqueous solution container box with the concentration of 4% above a glassware filled with pathological specimen tissues in the thermostat, controlling the flow of the paraformaldehyde aqueous solution at the bottom of the container box through a liquid electromagnetic valve, and intelligently controlling the delayed opening time of the liquid electromagnetic valve by adopting a time relay to supplement the paraformaldehyde aqueous solution into the glassware;
step five, storing the thermostat in a vacuum environment, sealing the thermostat, placing the thermostat in a vacuum box, closing a box door of the vacuum box, and vacuumizing the interior of the vacuum box by using a vacuum pump;
and step six, placing labels, namely adding the labels to the types and the placing time of the pathological specimen tissues on the surface of the vacuum box after the storage is finished, and explaining the attention points of the pathological specimen tissues.
In a preferred embodiment, the pathological specimen is sampled at room temperature in the first step, and forceps or other medical tools are used to insert the pathological specimen tissue into the glass vessel.
In a preferred embodiment, the paraformaldehyde solution in step two is poured into the glass vessel after being measured in volume by a test tube or a beaker container.
In a preferred embodiment, before the glassware in the third step is placed in the incubator, rubber protective strips are added on the edges of the glassware.
In a preferred embodiment, the time delay interval of each filling of the aqueous paraformaldehyde solution in the fourth step is equal, and the amount of the aqueous paraformaldehyde solution filled in each filling is one fifth of the capacity of the aqueous paraformaldehyde solution in the glassware.
In a preferred embodiment, the vacuum pump does not need to completely evacuate the internal air of the vacuum box during the evacuation in the step five, so as to reduce the oxygen content around the oven.
In a preferred embodiment, in the sixth step, the label is attached to the outer surface of the vacuum box through glue or placed inside a reserved label slot on the surface of the vacuum box.
Compared with the prior art, the invention has the beneficial effects that:
the pathological specimen preservation method designed by the invention is characterized in that a glass vessel filled with pathological specimen tissues is placed in a thermostat, the interior of the thermostat is intelligently controlled within a normal temperature range, a rubber protective strip is additionally arranged on the edge of the glass vessel before the glass vessel is placed in the thermostat, collision is avoided during placement, meanwhile, a paraformaldehyde aqueous solution container box with the concentration of 4% is fixed above the glass vessel filled with pathological specimen tissues in the thermostat, the bottom of the container box controls the flow of the paraformaldehyde aqueous solution through a liquid electromagnetic valve, a time relay is adopted to intelligently control the delayed opening time of the liquid electromagnetic valve to supplement the paraformaldehyde aqueous solution into the glass vessel, wherein the delayed intervals of filling the paraformaldehyde aqueous solution every time are equal, and the amount of the paraformaldehyde aqueous solution filled every time is one fifth of the volume of the paraformaldehyde aqueous solution in the glass vessel, avoid depositing the inside paraformaldehyde aqueous solution oxidation of in-process thermostated container and influence follow-up saving, simultaneously, place in the vacuum chamber after the thermostated container is airtight, close vacuum chamber case door after, utilize the vacuum pump to carry out the evacuation to the inside of vacuum chamber, wherein, the vacuum pump need not to find time the inside air of vacuum chamber completely when the evacuation to reduce the oxygen content around the thermostated container and be accurate, and then the save time of effectual extension pathology sample.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows:
the invention provides a pathological specimen preservation method, which specifically comprises the following steps:
sampling a pathological specimen, namely immediately placing the pathological specimen in a glass vessel after the tissue of the pathological specimen is excised and separated, and preparing to carry out fixation treatment by using a fixing solution, wherein the sampling of the pathological specimen is carried out at a temperature lower than the normal temperature, and the tissue of the pathological specimen is placed in the glass vessel by adopting tweezers or other medical tools;
fixing a pathological specimen, pouring a 4% paraformaldehyde aqueous solution into a glass vessel, and controlling the amount of the paraformaldehyde aqueous solution to be more than five times of the amount of a pathological specimen tissue, wherein the paraformaldehyde aqueous solution is poured into the glass vessel after the volume is measured by a test tube or a beaker container;
step three, storing at a temperature lower than normal temperature, namely placing the glassware filled with pathological specimen tissues in a constant temperature box, and intelligently regulating and controlling the interior of the constant temperature box to be in a range lower than the normal temperature, wherein rubber protective strips are required to be additionally arranged on the edge of the glassware before the glassware is placed in the constant temperature box;
step four, filling a paraformaldehyde aqueous solution, namely fixing a paraformaldehyde aqueous solution container box with the concentration of 4% above a glassware filled with pathological specimen tissues in a thermostat, controlling the flow of the paraformaldehyde aqueous solution at the bottom of the container box through a liquid electromagnetic valve, and intelligently controlling the delayed opening time of the liquid electromagnetic valve by adopting a time relay to supplement the paraformaldehyde aqueous solution into the glassware, wherein the delayed intervals of filling the paraformaldehyde aqueous solution every time are equal, and the amount of the paraformaldehyde aqueous solution filled every time is one fifth of the volume of the paraformaldehyde aqueous solution in the glassware;
step five, storing the thermostat in a vacuum environment, placing the thermostat in the vacuum box after sealing, and vacuumizing the interior of the vacuum box by using a vacuum pump after closing a box door of the vacuum box, wherein the vacuum pump does not need to completely evacuate the air in the vacuum box during vacuumizing so as to reduce the oxygen content around the thermostat;
and step six, placing a label, after the storage is finished, additionally installing the label on the type and the placing time of the pathological specimen tissue on the surface of the vacuum box, and explaining the attention of the pathological specimen tissue, wherein the label is attached to the outer surface of the vacuum box through glue or placed in a reserved label clamping groove on the surface of the vacuum box.
Example two:
the invention provides a pathological specimen preservation method, which specifically comprises the following steps:
sampling a pathological specimen, namely immediately placing the pathological specimen in a glass vessel after the tissue of the pathological specimen is excised and separated, and preparing to carry out fixation treatment by using a fixing solution, wherein the sampling of the pathological specimen is carried out at normal temperature, and the tissue of the pathological specimen is placed in the glass vessel by adopting tweezers or other medical tools;
fixing a pathological specimen, pouring a 4% paraformaldehyde aqueous solution into a glass vessel, and controlling the amount of the paraformaldehyde aqueous solution to be more than five times of the amount of a pathological specimen tissue, wherein the paraformaldehyde aqueous solution is poured into the glass vessel after the volume is measured by a test tube or a beaker container;
step three, preserving at normal temperature, namely placing the glassware filled with pathological specimen tissues in a constant temperature box, wherein the interior of the constant temperature box is intelligently regulated and controlled within the normal temperature range, and rubber protective strips are required to be additionally arranged on the edge of the glassware before the glassware is placed in the constant temperature box;
step four, filling a paraformaldehyde aqueous solution, namely fixing a paraformaldehyde aqueous solution container box with the concentration of 4% above a glassware filled with pathological specimen tissues in a thermostat, controlling the flow of the paraformaldehyde aqueous solution at the bottom of the container box through a liquid electromagnetic valve, and intelligently controlling the delayed opening time of the liquid electromagnetic valve by adopting a time relay to supplement the paraformaldehyde aqueous solution into the glassware, wherein the delayed intervals of filling the paraformaldehyde aqueous solution every time are equal, and the amount of the paraformaldehyde aqueous solution filled every time is one fifth of the volume of the paraformaldehyde aqueous solution in the glassware;
step five, storing the thermostat in a vacuum environment, placing the thermostat in the vacuum box after sealing, and vacuumizing the interior of the vacuum box by using a vacuum pump after closing a box door of the vacuum box, wherein the vacuum pump does not need to completely evacuate the air in the vacuum box during vacuumizing so as to reduce the oxygen content around the thermostat;
and step six, placing a label, after the storage is finished, additionally installing the label on the type and the placing time of the pathological specimen tissue on the surface of the vacuum box, and explaining the attention of the pathological specimen tissue, wherein the label is attached to the outer surface of the vacuum box through glue or placed in a reserved label clamping groove on the surface of the vacuum box.
Example three:
the invention provides a pathological specimen preservation method, which specifically comprises the following steps:
sampling a pathological specimen, namely immediately placing the pathological specimen in a glass vessel after the tissue of the pathological specimen is excised and separated, and preparing to carry out fixation treatment by using a fixing solution, wherein the sampling of the pathological specimen is carried out at a temperature higher than the normal temperature, and the tissue of the pathological specimen is placed in the glass vessel by adopting tweezers or other medical tools;
fixing a pathological specimen, pouring a 4% paraformaldehyde aqueous solution into a glass vessel, and controlling the amount of the paraformaldehyde aqueous solution to be more than five times of the amount of a pathological specimen tissue, wherein the paraformaldehyde aqueous solution is poured into the glass vessel after the volume is measured by a test tube or a beaker container;
step three, preserving at a temperature higher than normal temperature, namely placing the glassware filled with pathological specimen tissues in a thermostat, wherein the interior of the thermostat is intelligently controlled to be in a temperature range higher than the normal temperature, and rubber protective strips are required to be additionally arranged on the edge of the glassware before the glassware is placed in the thermostat;
step four, filling a paraformaldehyde aqueous solution, namely fixing a paraformaldehyde aqueous solution container box with the concentration of 4% above a glassware filled with pathological specimen tissues in a thermostat, controlling the flow of the paraformaldehyde aqueous solution at the bottom of the container box through a liquid electromagnetic valve, and intelligently controlling the delayed opening time of the liquid electromagnetic valve by adopting a time relay to supplement the paraformaldehyde aqueous solution into the glassware, wherein the delayed intervals of filling the paraformaldehyde aqueous solution every time are equal, and the amount of the paraformaldehyde aqueous solution filled every time is one fifth of the volume of the paraformaldehyde aqueous solution in the glassware;
step five, storing the thermostat in a vacuum environment, placing the thermostat in the vacuum box after sealing, and vacuumizing the interior of the vacuum box by using a vacuum pump after closing a box door of the vacuum box, wherein the vacuum pump does not need to completely evacuate the air in the vacuum box during vacuumizing so as to reduce the oxygen content around the thermostat;
and step six, placing a label, after the storage is finished, additionally installing the label on the type and the placing time of the pathological specimen tissue on the surface of the vacuum box, and explaining the attention of the pathological specimen tissue, wherein the label is attached to the outer surface of the vacuum box through glue or placed in a reserved label clamping groove on the surface of the vacuum box.
The pathological specimens stored in the embodiments 1 to 3 are taken out and respectively tested, under the conditions of different temperatures and the same other conditions, the pathological specimen storage effect is better under the normal temperature condition in the embodiment 2, in the preservation method of the pathological specimen, the glassware filled with the pathological specimen tissues is placed in the incubator in the use process, the inside of the incubator is intelligently regulated and controlled within the normal temperature range, wherein, a rubber protective strip is additionally arranged on the edge of the glassware before the glassware is placed in the incubator to avoid collision during placement, meanwhile, a paraformaldehyde aqueous solution container box with the concentration of 4 percent is fixed above the glassware filled with the pathological specimen tissues in the incubator, the bottom of the container box controls the flow discharge of the paraformaldehyde aqueous solution through a liquid electromagnetic valve, and the delayed opening time of the liquid electromagnetic valve is intelligently controlled by a time relay, supply paraformaldehyde aqueous solution to glassware's inside, wherein, the time delay interval of filling paraformaldehyde aqueous solution at every turn equals, and the amount of the paraformaldehyde aqueous solution of every turn filling is one fifth of glassware's inside paraformaldehyde aqueous solution capacity, avoid depositing the inside paraformaldehyde aqueous solution oxidation of glassware influence follow-up saving in-process thermostated container, and simultaneously, place in the vacuum chamber after the thermostated container is airtight, after closing vacuum chamber door, utilize the vacuum pump to carry out the evacuation to the inside of vacuum chamber, wherein, the vacuum pump need not to evacuate the inside air of vacuum chamber completely when the evacuation, with reduce the oxygen content around the thermostated container as the standard, and then the save time of effectual extension pathology sample.
The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (7)
1. A pathological specimen preservation method is characterized by comprising the following steps:
sampling a pathological specimen, namely immediately placing the pathological specimen in a glass vessel after the tissue of the pathological specimen is excised and isolated, and preparing to carry out fixation treatment by using a fixing solution;
fixing a pathological specimen, namely pouring a 4% paraformaldehyde aqueous solution into a glass vessel, and controlling the amount of the paraformaldehyde aqueous solution to be more than five times of the amount of the pathological specimen tissue;
step three, preserving at normal temperature, namely placing the glassware filled with pathological specimen tissues in a constant temperature box, and intelligently regulating and controlling the interior of the constant temperature box within a normal temperature range;
filling a paraformaldehyde aqueous solution, namely fixing a paraformaldehyde aqueous solution container box with the concentration of 4% above a glassware filled with pathological specimen tissues in the thermostat, controlling the flow of the paraformaldehyde aqueous solution at the bottom of the container box through a liquid electromagnetic valve, and intelligently controlling the delayed opening time of the liquid electromagnetic valve by adopting a time relay to supplement the paraformaldehyde aqueous solution into the glassware;
step five, storing the thermostat in a vacuum environment, sealing the thermostat, placing the thermostat in a vacuum box, closing a box door of the vacuum box, and vacuumizing the interior of the vacuum box by using a vacuum pump;
and step six, placing labels, namely adding the labels to the types and the placing time of the pathological specimen tissues on the surface of the vacuum box after the storage is finished, and explaining the attention points of the pathological specimen tissues.
2. The method for preserving a pathological specimen according to claim 1, wherein: and in the first step, the pathological specimen is sampled at room normal temperature, and the pathological specimen tissue is placed in a glass vessel by using tweezers or other medical tools.
3. The method for preserving a pathological specimen according to claim 1, wherein: and in the second step, the paraformaldehyde aqueous solution is poured into the glass ware after the volume is measured by a test tube or a beaker container.
4. The method for preserving a pathological specimen according to claim 1, wherein: and in the third step, before the glassware is placed in the thermostat, rubber protective strips are additionally arranged on the edges of the glassware.
5. The method for preserving a pathological specimen according to claim 1, wherein: in the fourth step, the time delay intervals of filling the paraformaldehyde aqueous solution every time are equal, and the amount of the paraformaldehyde aqueous solution filled every time is one fifth of the capacity of the paraformaldehyde aqueous solution in the glassware.
6. The method for preserving a pathological specimen according to claim 1, wherein: in the fifth step, the vacuum pump does not need to completely evacuate the air in the vacuum box during vacuumizing so as to reduce the oxygen content around the constant temperature box.
7. The method for preserving a pathological specimen according to claim 1, wherein: and in the sixth step, the label is attached to the outer surface of the vacuum box through glue or placed in a reserved label clamping groove on the surface of the vacuum box.
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| CN202011050863.1A CN112193625A (en) | 2020-09-29 | 2020-09-29 | Pathological specimen preservation method |
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| CN202011050863.1A CN112193625A (en) | 2020-09-29 | 2020-09-29 | Pathological specimen preservation method |
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Application publication date: 20210108 |