CN107367407A - A kind of Pathologic specimen is fixed and dehydration treatment method - Google Patents
A kind of Pathologic specimen is fixed and dehydration treatment method Download PDFInfo
- Publication number
- CN107367407A CN107367407A CN201710380143.3A CN201710380143A CN107367407A CN 107367407 A CN107367407 A CN 107367407A CN 201710380143 A CN201710380143 A CN 201710380143A CN 107367407 A CN107367407 A CN 107367407A
- Authority
- CN
- China
- Prior art keywords
- pathologic specimen
- tissue
- waxdip
- fixed
- minutes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000001575 pathological effect Effects 0.000 title claims abstract description 69
- 208000005156 Dehydration Diseases 0.000 title claims abstract description 21
- 230000018044 dehydration Effects 0.000 title claims abstract description 21
- 238000006297 dehydration reaction Methods 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000008096 xylene Substances 0.000 claims abstract description 18
- 230000008595 infiltration Effects 0.000 claims abstract description 9
- 238000001764 infiltration Methods 0.000 claims abstract description 9
- 230000035515 penetration Effects 0.000 claims abstract description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 35
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 24
- 239000003153 chemical reaction reagent Substances 0.000 claims description 4
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 4
- 239000012530 fluid Substances 0.000 claims description 4
- 239000012188 paraffin wax Substances 0.000 claims description 4
- 238000005070 sampling Methods 0.000 claims description 4
- 229910000403 monosodium phosphate Inorganic materials 0.000 claims description 3
- 235000019799 monosodium phosphate Nutrition 0.000 claims description 3
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims description 3
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 claims description 2
- 239000008280 blood Substances 0.000 abstract description 4
- 210000004369 blood Anatomy 0.000 abstract description 4
- 239000012535 impurity Substances 0.000 abstract description 4
- 238000011010 flushing procedure Methods 0.000 abstract description 3
- 238000004140 cleaning Methods 0.000 description 4
- 238000007654 immersion Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000012466 permeate Substances 0.000 description 3
- 239000001993 wax Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 230000007170 pathology Effects 0.000 description 2
- 206010054949 Metaplasia Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000015689 metaplastic ossification Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- NFIYTPYOYDDLGO-UHFFFAOYSA-N phosphoric acid;sodium Chemical compound [Na].OP(O)(O)=O NFIYTPYOYDDLGO-UHFFFAOYSA-N 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/305—Fixative compositions
Abstract
Fixed the invention discloses a kind of Pathologic specimen and dehydration treatment method, including following steps：Pathologic specimen tissue is fixed, the flushing of Pathologic specimen tissue, Pathologic specimen tissue dewatering, Pathologic specimen tissue infiltration, Pathologic specimen tissue waxdip.The present invention with fixer Pathologic specimen tissue using first being fixed, then is cleaned up with running water, can effectively be removed the impurity such as blood stains and impure fixer in tissue, be ensure that the purity of the fixer in dewaterer；Serial dehydration is used in dewater treatment, dehydration is complete, uses xylene solution gradient penetration before waxdip, can effectively carry out waxdip, and waxdip is uniform, the making for being easy to the later stage to cut into slices.
Description
Technical field
The invention belongs to the technical field that Pathologic specimen makes, more particularly to a kind of convenient sample is fixed and dewater treatment side
Method.
Background technology
During the bearing of Pathologic specimen, tissue is fixed and dehydration is committed step, and good fixed dehydration could be protected
Card preparation of specimen is smoothed out, and almost pathology department all routinely applies automatic tissue dehydration apparatus in the prior art, but sometimes
Because exception occur in power failure or computer program, cause dewaterer clocking capability disorderly or break down, be easily caused convenient sample
The processing of basic stitch can not be normally carried out or the gray-off of sample, directly affects the diagnosis of later stage pathology, can not to some
Again the sample drawn materials, this will be fatal damage.Therefore, it is not ten thousand to carry out dehydration to Pathologic specimen using automatic dehydrator
What none lost, therefore, how can just make high-quality pathological section, it should in the fixation of Pathologic specimen and dehydration process
It is further optimized.
The content of the invention
The present invention solves Pathologic specimen of the prior art sample metaplasia or dehydration in fixation and dewater treatment
Not thoroughly, the problems such as waxdip is insufficient.
In order to solve the above-mentioned technical problem, fix the invention provides a kind of Pathologic specimen and dehydration treatment method, including
Following steps：
(1) Pathologic specimen tissue is fixed：Pathologic specimen is placed in the special fixed fluid cylinder on sampling platform and uses fixer
Immersion；
(2) Pathologic specimen tissue rinses：Pathologic specimen tissue is rinsed with running water；
(3) Pathologic specimen tissue dewatering：Pathologic specimen tissue is dehydrated using automatic dehydrator, successively with fixation
Liquid, AF solution, ethanol gradient solution are replaced step by step, and the AF solution is 10% formaldehyde alcohol reagent, the ethanol gradient solution
It is 95% → 95% → 95% → 100% → 100% → 100% that volume ratio in each gradient shared by ethanol, which is respectively, described every
Individual to need time swap be 30-40 minutes, while is pressurizeed；
(4) Pathologic specimen tissue infiltration：Successively permeated twice using xylene solution, when each xylene solution permeates
Between be 30-40 minutes, while pressurizeed；
(5) Pathologic specimen tissue waxdip：Pathologic specimen tissue is soaked three times step by step using paraffin under vacuum conditions
Wax, the preceding time of waxdip twice are respectively 30-40 minutes, and the rear waxdip time is 40-45 minutes, and respectively the temperature of waxdip is step by step
64℃。
Preferably, the temperature in the Pathologic specimen tissue dewatering, Pathologic specimen tissue infiltration step is controlled as 37 DEG C
Constant temperature.
Preferably, the composition of the fixer is：Formaldehyde, water, sodium dihydrogen phosphate, disodium hydrogen phosphate.
Preferably, the automatic dehydrator is entered using xylene solution, 95% alcohol, water successively after step (5) is completed
Row cleaning, 18-20 minutes are cleaned using xylene solution, using 95% alcohol washes 18-20 minutes, using 5-8 points of water cleaning
Clock.
Compared with prior art, the present invention achieves following beneficial effect：The present invention uses and first uses fixer Pathologic specimen
Tissue is fixed, then is cleaned up with running water, can effectively be removed the impurity such as blood stains and impure fixer in tissue, be ensure that
The purity of fixer in dewaterer；Serial dehydration is used in dewater treatment, dehydration is complete, and xylene solution is used before waxdip
Gradient penetration, waxdip can be effectively carried out, waxdip is uniform, the making for being easy to the later stage to cut into slices.
Embodiment
Some vocabulary has such as been used to censure specific components among specification and claim.Those skilled in the art should
It is understood that hardware manufacturer may call same component with different nouns.This specification and claims are not with name
The difference of title is used as the mode for distinguishing component, but is used as the criterion of differentiation with the difference of component functionally.Specification
Subsequent descriptions for implement the application better embodiment, so it is described description be for the purpose of the rule for illustrating the application,
It is not limited to scope of the present application.The protection domain of the application is worked as to be defined depending on appended claims institute defender.
The application is described in further detail below, but not as the restriction to the application.
Embodiment one：
A kind of Pathologic specimen is fixed and dehydration treatment method, including following steps：
(1) Pathologic specimen tissue is fixed：Pathologic specimen is placed in the special fixed fluid cylinder on sampling platform and uses fixer
Immersion, soak time determine according to the property of Pathologic specimen tissue；
(2) Pathologic specimen tissue rinses：Pathologic specimen tissue is rinsed with running water, uses a small amount of running water during flushing
Can, in order to wash away the impurity such as the blood stains in tissue and impure fixer, to ensure the fixer in dewaterer
Purity；
(3) Pathologic specimen tissue dewatering：Pathologic specimen tissue is dehydrated using automatic dehydrator, successively with fixation
Liquid, AF solution, ethanol gradient solution are replaced step by step, and the AF solution is 10% formaldehyde alcohol reagent, the ethanol gradient solution
It is 95% → 95% → 95% → 100% → 100% → 100% that volume ratio in each gradient shared by ethanol, which is respectively, described every
It is individual to need time swap as 30 minutes, while pressurizeed；
(4) Pathologic specimen tissue infiltration：Successively permeated twice using xylene solution, when each xylene solution permeates
Between be 30 minutes, while pressurizeed；
(5) Pathologic specimen tissue waxdip：Pathologic specimen tissue is soaked three times step by step using paraffin under vacuum conditions
Wax, the preceding time of waxdip twice are respectively 30 minutes, and the rear waxdip time is 40 minutes, and respectively the temperature of waxdip is 64 DEG C step by step.
In the present embodiment, the temperature in the Pathologic specimen tissue dewatering, Pathologic specimen tissue infiltration step, which controls, is
37 DEG C of constant temperature, and in operation early stage of automatic dehydrator by temperature control be 37 DEG C of constant temperature.
In the present embodiment, the composition of fixer is：40% formalin 100ml, water 900ml, disodium hydrogen phosphate 4g, phosphoric acid
Sodium dihydrogen 6.5g, what is be made into after well mixed is 4% formalin, traditionally referred to as 10% neutral formalin solution.
In the present embodiment, automatic dehydrator is entered using xylene solution, 95% alcohol, water successively after step (5) is completed
Row cleaning, is cleaned 18 minutes using xylene solution, using 95% alcohol washes 18 minutes, is cleaned 5 minutes using water.
Embodiment two：
A kind of Pathologic specimen is fixed and dehydration treatment method, including following steps：
(1) Pathologic specimen tissue is fixed：Pathologic specimen is placed in the special fixed fluid cylinder on sampling platform and uses fixer
Immersion, soak time determine according to the property of Pathologic specimen tissue；
(2) Pathologic specimen tissue rinses：Pathologic specimen tissue is rinsed with running water, uses a small amount of running water during flushing
Can, in order to wash away the impurity such as the blood stains in tissue and impure fixer, to ensure the fixer in dewaterer
Purity；
(3) Pathologic specimen tissue dewatering：Pathologic specimen tissue is dehydrated using automatic dehydrator, and fill in instrument to make
With record sheet, successively replaced step by step with fixer, AF solution, ethanol gradient solution, AF solution is 10% formaldehyde alcohol reagent, second
Volume ratio in each gradient of alcohol gradient solution shared by ethanol is respectively 95% → 95% → 95% → 100% → 100% →
100%, it is 35 minutes each to need time swap, while is pressurizeed；
(4) Pathologic specimen tissue infiltration：Successively permeated twice using xylene solution, when each xylene solution permeates
Between be 35 minutes, while pressurizeed；
(5) Pathologic specimen tissue waxdip：Pathologic specimen tissue is soaked three times step by step using paraffin under vacuum conditions
Wax, the preceding time of waxdip twice are respectively 35 minutes, and the rear waxdip time is 45 minutes, and respectively the temperature of waxdip is 64 DEG C step by step.
In the present embodiment, the temperature in the Pathologic specimen tissue dewatering, Pathologic specimen tissue infiltration step, which controls, is
37 DEG C of constant temperature, and in operation early stage of automatic dehydrator by temperature control be 37 DEG C of constant temperature.
In the present embodiment, the composition of fixer is：
40% formalin 100ml, water 900ml, disodium hydrogen phosphate 4g, sodium dihydrogen phosphate 6.5g, it is made into after well mixed
Be 4% formalin, traditionally referred to as 10% neutral formalin solution.
In the present embodiment, automatic dehydrator is entered using xylene solution, 95% alcohol, water successively after step (5) is completed
Row cleaning, is cleaned 20 minutes using xylene solution, using 95% alcohol washes 20 minutes, is cleaned 8 minutes using water.
Some preferred embodiments of the application have shown and described in described above, but as previously described, it should be understood that the application
Be not limited to form disclosed herein, be not to be taken as the exclusion to other embodiment, and available for various other combinations,
Modification and environment, and above-mentioned teaching or the technology or knowledge of association area can be passed through in application contemplated scope described herein
It is modified., then all should be in this Shen and the change and change that those skilled in the art are carried out do not depart from spirit and scope
Please be in the protection domain of appended claims.
Claims (4)
1. a kind of Pathologic specimen is fixed and dehydration treatment method, it is characterised in that including following steps：
(1) Pathologic specimen tissue is fixed：Pathologic specimen is placed in the special fixed fluid cylinder on sampling platform and soaked with fixer；
(2) Pathologic specimen tissue rinses：Pathologic specimen tissue is rinsed with running water；
(3) Pathologic specimen tissue dewatering：Pathologic specimen tissue is dehydrated using automatic dehydrator, successively with fixer, AF
Solution, ethanol gradient solution are replaced step by step, and the AF solution is 10% formaldehyde alcohol reagent, each gradient of ethanol gradient solution
It is 95% → 95% → 95% → 100% → 100% → 100% that volume ratio shared by middle ethanol, which is respectively, described each to put
It is 30-40 minutes to change the time, while is pressurizeed；
(4) Pathologic specimen tissue infiltration：Successively permeated twice using xylene solution, each xylene solution time of penetration is
30-40 minutes, while pressurizeed；
(5) Pathologic specimen tissue waxdip：Waxdip three times is carried out to Pathologic specimen tissue using paraffin step by step under vacuum conditions, it is preceding
The waxdip time is respectively 30-40 minutes twice, and the rear waxdip time is 40-45 minutes, and respectively the temperature of waxdip is 64 DEG C step by step.
2. a kind of Pathologic specimen as claimed in claim 1 is fixed and dehydration treatment method, it is characterised in that the Pathologic specimen
Temperature in tissue dewatering, Pathologic specimen tissue infiltration step controls the constant temperature for 37 DEG C.
3. a kind of Pathologic specimen as claimed in claim 1 is fixed and dehydration treatment method, it is characterised in that the fixer
Form and be：Formaldehyde, water, sodium dihydrogen phosphate, disodium hydrogen phosphate.
4. a kind of Pathologic specimen as claimed in claim 1 is fixed and dehydration treatment method, it is characterised in that the automatic dehydration
Machine is cleaned using xylene solution, 95% alcohol, water successively after step (5) is completed, and 18- is cleaned using xylene solution
20 minutes, using 95% alcohol washes 18-20 minutes, 5-8 minutes are cleaned using water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710380143.3A CN107367407B (en) | 2017-05-25 | 2017-05-25 | Pathological specimen fixing and dehydrating treatment method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710380143.3A CN107367407B (en) | 2017-05-25 | 2017-05-25 | Pathological specimen fixing and dehydrating treatment method |
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| Publication Number | Publication Date |
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| CN107367407A true CN107367407A (en) | 2017-11-21 |
| CN107367407B CN107367407B (en) | 2020-04-24 |
Family
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| CN201710380143.3A Active CN107367407B (en) | 2017-05-25 | 2017-05-25 | Pathological specimen fixing and dehydrating treatment method |
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Cited By (3)
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| CN112193625A (en) * | 2020-09-29 | 2021-01-08 | 湖州市中医院 | Pathological specimen preservation method |
| CN117337827A (en) * | 2023-10-18 | 2024-01-05 | 温州市倍可特医疗器械有限公司 | Method for inhibiting formaldehyde volatilization in tissue fixing liquid and application thereof |
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| CN112193625A (en) * | 2020-09-29 | 2021-01-08 | 湖州市中医院 | Pathological specimen preservation method |
| CN117337827A (en) * | 2023-10-18 | 2024-01-05 | 温州市倍可特医疗器械有限公司 | Method for inhibiting formaldehyde volatilization in tissue fixing liquid and application thereof |
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| CN107367407B (en) | 2020-04-24 |
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Address after: 410000 No. 101-801, floor 1-8, building d1-d2, jinruilugu science and Technology Park, No. 28, Lutian Road, high tech Development Zone, Changsha, Hunan Patentee after: Changsha Jinyu medical laboratory Co., Ltd Address before: 410000 No. 101-801, floor 1-8, building d1-d2, jinruilugu science and Technology Park, No. 28, Lutian Road, high tech Development Zone, Changsha, Hunan Patentee before: CHANGSHA KINGMED MEDICAL DIAGNOSTICS INSTITUTE Co.,Ltd. |