CN112175806B - Microorganism sampling and storing integrated container - Google Patents
Microorganism sampling and storing integrated container Download PDFInfo
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- CN112175806B CN112175806B CN202010881644.1A CN202010881644A CN112175806B CN 112175806 B CN112175806 B CN 112175806B CN 202010881644 A CN202010881644 A CN 202010881644A CN 112175806 B CN112175806 B CN 112175806B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
- C12M33/07—Dosage or metering devices therefore
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/22—Means for packing or storing viable microorganisms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W90/00—Enabling technologies or technologies with a potential or indirect contribution to greenhouse gas [GHG] emissions mitigation
- Y02W90/10—Bio-packaging, e.g. packing containers made from renewable resources or bio-plastics
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention provides a microorganism sampling and storing integrated container, and mainly relates to the technical field of microorganism containers. The utility model provides a microorganism sample-storage integral type container, includes container main part, piston, top cap, bottom, set up the baffle in the container main part, threaded hole has been seted up at the baffle middle part, liquid outlet, leakage fluid dram have been seted up to one side on the baffle, set up the discharge pipe on the container main part outer wall, T shape through-hole has been seted up at the piston middle part, the reservoir is a plurality of has been seted up to the piston bottom, set up the rotating handle on the piston outer wall, top cap bottom sets up T shape bolt, piston sliding connection is in the container main part, the bottom passes through threaded connection in container main part bottom. The invention has the beneficial effects that: through the structural design of the invention, the storage of microorganisms is convenient, the accurate control of the sample sampling amount on the sealed basis can be ensured, and the container can be cleaned conveniently after the device is disassembled.
Description
Technical Field
The invention mainly relates to the technical field of microbial containers, in particular to a microbial sampling and storing integrated container.
Background
At present, a plurality of glass containers are used as storage equipment of microorganism samples, and the glass containers have poor sealing performance due to the fact that the structures of the glass containers are too simple, so that the microorganism samples in the glass containers are easy to leak, and unnecessary troubles are brought; in addition, after the microbial specimens are all taken out from the storage containers, since the microbial specimens are stored in the storage containers for a long time, a lot of sediment is adhered to the bottoms of the storage containers, and in order to ensure the tightness of the interiors of the storage containers, the diameters of the feed inlets of the containers are generally made smaller, which brings unnecessary trouble to cleaning the bottoms of the storage containers, and the bottoms of the storage containers are likely to be unable to be completely cleaned.
Most of sampling work is completed by people, and the existing sampler is not firm in the sampling process, is easy to pollute during sampling, and is difficult to ensure accurate and standard microbial sampling data, so that a hospital microbial sample storage container is needed to facilitate sampling and storage of microbial samples. The invention provides a microorganism sampling and storing integrated container and a use method thereof, which can accurately control the sampling amount and effectively prevent pollution during sampling, and the container integrates sampling and storing.
Disclosure of Invention
In order to solve the defects in the prior art, the invention provides the microorganism sampling and storing integrated container, and through the structural design of the microorganism sampling and storing integrated container, the microorganism storage is convenient, the accurate control of the sample sampling amount on the sealed basis can be ensured, the pollution in the sampling process is prevented, the microorganism sampling and storing integrated is realized, and meanwhile, the container is convenient to clean after the device is disassembled.
The invention aims to achieve the aim, and the aim is achieved by the following technical scheme:
the utility model provides an integral type container is stored in microorganism sample, includes container main part, piston, top cap, bottom, set up the baffle in the container main part, threaded hole has been seted up at the baffle middle part, the liquid outlet has been seted up to one side on the baffle, the liquid outlet has been seted up to one side of keeping away from the liquid outlet on the baffle, set up the discharge pipe on the container main part outer wall, the T-shaped through-hole that runs through the piston has been seted up at the piston middle part, the cistern a plurality of has been seted up to the piston bottom, set up the stem on the piston outer wall, the top cap bottom sets up T-shaped bolt, piston sliding connection is on container main part upper portion inner wall, T-shaped through-hole is passed in the piston at T-shaped bolt middle part, the screw hole at baffle middle part is connected to T-shaped bolt bottom, the bottom passes through threaded connection in container main part bottom.
The discharge pipe is communicated with the liquid outlet, a liquid suction ball is arranged in the middle of the discharge pipe, and the discharge pipe and the liquid suction ball are of hose structures.
The reservoir includes: the liquid storage tank I, the liquid storage tank II and the liquid storage tank III are arranged symmetrically and circumferentially about the longitudinal central axis of the piston, the volumes of the liquid storage tank I, the liquid storage tank II and the liquid storage tank III are different, and the opening shape of the liquid storage tank is the same as that of the liquid outlet.
The top of the outer wall of the container main body is provided with a plurality of mark scale marks.
The T-shaped bolt comprises a thick handle and a thin handle, wherein the thick handle and the thin handle are of an integrated structure, and a first thread is arranged at the bottom of the thin handle.
The threaded hole is internally provided with a second thread, the inner wall of the bottom of the container main body is provided with a third thread, and the upper part of the bottom cover is provided with a fourth thread.
The first thread and the second thread are matched to enable the T-shaped bolt and the top cover to be connected to the container main body, the bottom of the thick handle tightly presses the piston on the partition plate in the container main body, and the third thread and the fourth thread are matched to enable the bottom cover to be connected to the bottom of the container main body.
Compared with the prior art, the invention has the beneficial effects that:
the invention can not only facilitate the storage of microorganisms through the structural design of the invention, but also ensure the accurate control of the sample sampling amount on the basis of sealing, prevent the pollution in the sampling process, realize the integration of microorganism sampling and storage, and simultaneously facilitate the cleaning of the container after the device is disassembled.
Drawings
FIG. 1 is a schematic view of a first view angle dispersion structure according to the present invention;
FIG. 2 is a schematic view of a second view angle dispersion structure according to the present invention;
FIG. 3 is a schematic diagram of the front view of the present invention;
FIG. 4 is a schematic view of the assembled structure of the present invention;
FIG. 5 is a schematic view showing the internal structure of the container body of the present invention;
FIG. 6 is a schematic view of the bottom structure of the piston of the present invention;
FIG. 7 is a schematic view of the upper structure of the piston of the present invention;
FIG. 8 is a schematic view of the structure of the top cover of the present invention;
FIG. 9 is a schematic view of the position of the liquid outlet and liquid outlet in the closed state of the piston of the present invention.
The reference numbers shown in the drawings: 1. a container body; 2. a piston; 3. a top cover; 4. a bottom cover; 10. marking scale marks; 11. a partition plate; 12. a threaded hole; 13. a liquid outlet; 14. a liquid outlet; 15. a discharge pipe; 151. a liquid suction ball; 21. t-shaped through holes; 22. a liquid storage tank; 221. a first liquid storage tank; 222. a liquid storage tank II; 223. a liquid storage tank III; 224. a liquid storage tank IV; 31. t-shaped bolts; 310. a first thread; 120. a second thread; 100. a third thread; 400. a thread IV; 311. a thick handle; 312. a thin handle; 333. a pressure surface; 210. force bearing surface.
Detailed Description
The invention will be further described with reference to the accompanying drawings and specific embodiments. It is to be understood that these examples are illustrative of the present invention and are not intended to limit the scope of the present invention. Further, it will be understood that various changes or modifications may be made by those skilled in the art after reading the teachings of the invention, and such equivalents are intended to fall within the scope of the invention as defined herein.
1-9, a microorganism sample stores integrative container, including container body 1, piston 2, top cap 3, bottom 4, set up baffle 11 in the container body 1, threaded hole 12 has been seted up at the baffle 11 middle part, liquid outlet 13 has been seted up to one side on the baffle 11, liquid outlet 14 has been seted up to one side of keeping away from liquid outlet 13 on the baffle 11, set up discharge pipe 15 on the container body 1 outer wall, T-shaped through-hole 21 that runs through the piston has been seted up at the piston 2 middle part, reservoir 22 has been seted up to the piston 2 bottom a plurality of, set up stem 23 on the piston 2 outer wall, top cap 3 bottom sets up T-shaped bolt 31, piston 2 sliding connection is on container body 1 upper portion inner wall, T-shaped bolt 31 middle part passes in the piston 2T-shaped through-hole 21, T-shaped bolt 31 bottom connects the threaded hole 12 at the baffle 11 middle part, bottom 4 passes through threaded connection in container body 1 bottom.
The discharge pipe 15 is communicated with the liquid outlet 14, a liquid suction ball 151 is arranged in the middle of the discharge pipe 15, and the discharge pipe 15 and the liquid suction ball 151 are of hose structures. When the concentration is large or other conditions liquid can not be discharged from the liquid outlet by itself, the liquid can be sucked out by using the liquid suction ball. The use method of the liquid suction ball comprises the steps of pinching the outlet of the liquid discharge pipe, pinching and loosening the liquid suction ball for a plurality of times, and loosening the outlet of the liquid discharge pipe to discharge liquid.
The reservoir 22 includes: the first liquid storage tank 221, the second liquid storage tank 222 and the third liquid storage tank 223, wherein the first liquid storage tank 221, the second liquid storage tank 222 and the third liquid storage tank 223 are symmetrically arranged in a circle mode about the longitudinal central axis of the piston 2, the volumes of the first liquid storage tank 221, the second liquid storage tank 222 and the third liquid storage tank 223 are different, and the opening shape of the liquid storage tank 22 is the same as that of the liquid outlet 13. The capacity of the first liquid storage tank is 3ml, the capacity of the second liquid storage tank is 6ml, the capacity of the third liquid storage tank is 5ml, the capacity of the fourth liquid storage tank is 8ml, the number and the capacity of different liquid storage tanks can be set according to the needs in practical application, and the liquid storage tanks with different numbers and capacities are all included in the protection scope of the invention.
The top of the outer wall of the container main body 1 is provided with a plurality of mark graduation marks 10. The device comprises four groups of scale marks, namely a first liquid storage tank, a third liquid storage tank, a fourth liquid storage tank, a third liquid storage tank, a fourth liquid storage tank and a closing section.
When the rotating handle 23 is positioned in the scale line range of the closing interval, as shown in fig. 9, the liquid outlet 13 and the liquid outlet 14 are both in a closing state, when the rotating handle 23 is positioned in the scale line of the first liquid storage tank, which enters the third liquid storage tank (3+) and is discharged (5-) from the third liquid storage tank, the liquid outlet 13 is opposite to the first liquid storage tank 221, the liquid outlet 14 is opposite to the third liquid storage tank 223, and other scale lines are used in the same way.
The T-shaped bolt 31 comprises a thick handle 311 and a thin handle 312, the thick handle 311 and the thin handle 312 are of an integrated structure, and a first thread 310 is arranged at the bottom of the thin handle 312. The threaded hole 12 is internally provided with a second thread 120, the inner wall of the bottom of the container main body 1 is provided with a third thread 100, and the upper part of the bottom cover 4 is provided with a fourth thread 400.
The first thread 310 is matched with the second thread 120 to enable the T-shaped bolt 31 and the top cover 3 to be connected to the container main body 1, the bottom of the thick handle 311 presses the piston 2 on the partition plate 11 in the container main body 1, and the third thread 100 is matched with the fourth thread 400 to enable the bottom cover 4 to be connected to the bottom of the container main body 1. After the piston is arranged in the container main body, the upper surface of the piston is slightly higher than the upper end surface of the container main body, and the outer side wall of the piston, which is higher than the upper part of the container main body, is a connecting position of the rotating handle.
After the top cover and the piston are assembled on the container main body, the annular surface of the thin handle protruding from the bottom of the thick handle of the T-shaped bolt is a pressure surface 333, and the pressure surface 333 compresses the stress surface 210 in the T-shaped through hole, so that the piston is compressed on the partition plate in the container main body, and the piston is fixed in the way, and the rotation resistance of the piston in the container main body is reduced on the basis of ensuring the compression of the piston due to the smaller stress area, so that the usability is improved.
If observation is needed in the microorganism preservation process, the container main body in the embodiment can adopt a transparent glass structure, so that the observation is convenient; the main body of the microbial container, which does not need to be observed, may be of a metal structure to improve sealability.
When the microbial disinfection device is used, after all the components are disassembled for disinfection treatment, the top cover and the piston are mounted on the container main body, the rotary handle is stirred to enable the piston to be in a closed state, the container is inverted, the microbial liquid to be stored is added from the bottom of the container main body, the bottom cover is covered and screwed up after the microbial liquid to be stored is added, and the container is stored in a proper temperature environment of microorganisms; when the quantitative sampling is needed, the rotating handle is shifted to the position of the liquid entering the scale mark of the liquid storage tank with corresponding capacity according to the need, then the container is inverted to enable the liquid storage tank to be filled with microorganism liquid, the rotating handle is shifted to the adjacent closing interval, the container is vertically arranged to continuously shift the rotating handle to the liquid outlet scale mark corresponding to the liquid storage tank according to the rotating direction of the piston in the previous step, so that the liquid is discharged from the liquid discharge pipe, if the liquid can not be automatically discharged, the liquid can be sucked out by using the liquid suction ball, the rotating handle is shifted to the closing interval after the sampling is finished, and the microorganism liquid is continuously stored, thereby realizing the sampling and storage of microorganisms in a whole.
In the sampling process of the microorganism sample, the air in the container is not directly communicated with the atmosphere, and meanwhile, the downward arrangement of the discharge pipe can effectively prevent microorganisms in the air from entering the container, so that the problem of microorganism pollution is effectively prevented.
Claims (5)
1. The utility model provides an integral type container is stored in microorganism sample, includes container body (1), piston (2), top cap (3), bottom (4), its characterized in that: set up baffle (11) in container main part (1), screw hole (12) have been seted up at baffle (11) middle part, liquid outlet (13) have been seted up to one side on baffle (11), liquid outlet (14) have been seted up to one side of keeping away from liquid outlet (13) on baffle (11), set up discharge pipe (15) on container main part (1) outer wall, T-shaped through-hole (21) that run through the piston have been seted up at piston (2) middle part, liquid storage tank (22) have been seted up at piston (2) bottom a plurality of, set up stem (23) on piston (2) outer wall, top cap (3) bottom sets up T-shaped bolt (31), piston (2) sliding connection is on container main part (1) upper portion inner wall, T-shaped through-hole (21) are passed in piston (2) at T-shaped bolt (31) middle part, screw hole (12) at baffle (11) middle part are connected to T-shaped bolt (31) bottom, bottom (4) are through threaded connection in container main part (1) bottom, discharge pipe (15) and liquid outlet (14) intercommunication, top (15) bottom setting up, discharge pipe (151) ball (151) are liquid absorbing ball (151), the top of the outer wall of the container main body (1) is provided with a plurality of mark graduation marks (10).
2. A microbiological sampling storage integrated container as set forth in claim 1 wherein: the reservoir (22) comprises: the liquid storage tank I (221), the liquid storage tank II (222) and the liquid storage tank III (223), wherein the liquid storage tank I (221), the liquid storage tank II (222) and the liquid storage tank III (223) are symmetrically arranged in a circle mode about the longitudinal central axis of the piston (2) and are different in volume, and the opening shape of the liquid storage tank (22) is the same as that of the liquid outlet (13).
3. A microbiological sampling storage integrated container as set forth in claim 1 wherein: the T-shaped bolt (31) comprises a thick handle (311) and a thin handle (312), wherein the thick handle (311) and the thin handle (312) are of an integrated structure, and a first thread (310) is formed at the bottom of the thin handle (312).
4. A microbiological sampling storage integrated container as set forth in claim 3 wherein: a second thread (120) is arranged in the threaded hole (12), a third thread (100) is arranged on the inner wall of the bottom of the container main body (1), and a fourth thread (400) is arranged on the upper portion of the bottom cover (4).
5. The microbiological sampling storage integrated container of claim 4 wherein: the first screw thread (310) is matched with the second screw thread (120) to enable the T-shaped bolt (31) and the top cover (3) to be connected to the container main body (1), the bottom of the thick handle (311) is used for pressing the piston (2) on the partition plate (11) in the container main body (1), and the third screw thread (100) is matched with the fourth screw thread (400) to enable the bottom cover (4) to be connected to the bottom of the container main body (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010881644.1A CN112175806B (en) | 2020-08-26 | 2020-08-26 | Microorganism sampling and storing integrated container |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010881644.1A CN112175806B (en) | 2020-08-26 | 2020-08-26 | Microorganism sampling and storing integrated container |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112175806A CN112175806A (en) | 2021-01-05 |
| CN112175806B true CN112175806B (en) | 2023-07-21 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202010881644.1A Active CN112175806B (en) | 2020-08-26 | 2020-08-26 | Microorganism sampling and storing integrated container |
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| CN (1) | CN112175806B (en) |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003065909A (en) * | 2001-08-23 | 2003-03-05 | Fujikin Inc | Sampling device |
| EP2378119A1 (en) * | 2010-04-15 | 2011-10-19 | Mmi Ag | Plunger pump with manual insertion possibility for volumes under a microlitre |
| CN209911050U (en) * | 2019-05-06 | 2020-01-07 | 韩云霞 | Specimen sampling tube for medical examination |
| CN211178670U (en) * | 2019-09-23 | 2020-08-04 | 长春晨裕生物医疗科技有限公司 | Sampling tube device for measuring liquid level of container |
| CN211213026U (en) * | 2019-10-29 | 2020-08-11 | 江苏雷利电机股份有限公司 | Detergent feeding device |
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| CN112175806A (en) | 2021-01-05 |
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