CN220564585U - Measuring device for microorganism respiration rate on solid culture dish - Google Patents
Measuring device for microorganism respiration rate on solid culture dish Download PDFInfo
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- CN220564585U CN220564585U CN202322081780.4U CN202322081780U CN220564585U CN 220564585 U CN220564585 U CN 220564585U CN 202322081780 U CN202322081780 U CN 202322081780U CN 220564585 U CN220564585 U CN 220564585U
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- culture dish
- solid culture
- groove
- diameter
- respiration rate
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- 239000007787 solid Substances 0.000 title claims abstract description 50
- 244000005700 microbiome Species 0.000 title claims abstract description 26
- 230000029058 respiratory gaseous exchange Effects 0.000 title claims abstract description 21
- 238000005070 sampling Methods 0.000 claims abstract description 15
- 230000002093 peripheral effect Effects 0.000 claims description 5
- 239000011521 glass Substances 0.000 claims description 4
- 230000000149 penetrating effect Effects 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 2
- 238000004868 gas analysis Methods 0.000 claims 2
- 238000005259 measurement Methods 0.000 abstract description 6
- 239000007789 gas Substances 0.000 description 27
- 238000007789 sealing Methods 0.000 description 7
- 230000008859 change Effects 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- 230000004060 metabolic process Effects 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 230000036284 oxygen consumption Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000000565 sealant Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model discloses a measuring device for the respiration rate of microorganisms on a solid culture dish, which relates to the technical field of microorganism culture and comprises the following components: the gas analyzer comprises a bearing box and a gas analyzer, wherein the bearing box comprises a base and a cover body, a cylindrical groove is formed in the base, a connecting part is in threaded connection with an internal thread of the groove, a placing space is formed between the bottom wall in the groove and the bottom end of the connecting part, the diameter of the placing space is not smaller than that of a solid culture dish, the thickness of the placing space is not smaller than that of the solid culture dish, and a sampling needle head of the gas analyzer is positioned in the placing space; the cover body is stretched into the groove to be connected with the groove, and the size of the placement space formed after the cover body is connected with the groove is designed based on the specification of the solid culture dish, so that the cover body occupies the space in the groove, the volume of the gas which can be contained in the groove is reduced on the basis of ensuring that the solid culture dish can be placed in the groove, and the measurement accuracy is improved.
Description
Technical Field
The utility model relates to the technical field of microorganism culture, in particular to a device for measuring the respiration rate of microorganisms on a solid culture dish.
Background
At present, the containers for measuring the respiration rate of microorganisms are all suitable for fluid or fluid-like culture systems (such as liquid culture medium or soil), and the specific scheme is as follows: the measurement of the microorganism respiration rate can be completed by using a glass bottle with a proper volume and sealing and connecting a gas component analyzer by using a rubber plug, but no proper measuring container is provided for accurately measuring the metabolism rate of microorganisms on a solid culture dish, and the technical difficulty is that: the quantity of the monoclonal bacterial colonies contained in the solid culture dish is limited, the total oxygen consumption of microorganisms in the metabolic process is low, when the gas volume in the closed container is large, the concentration change degree of the gas components caused by metabolism is small, the concentration change degree of the components such as oxygen in the container is difficult to accurately measure based on the precision of the existing gas component analyzer, namely the volume of the existing closed container which can be filled in the culture dish is large, the volume of the contained gas is large, and the concentration change of the components such as oxygen is difficult to accurately measure.
There is therefore a need for a device for measuring the respiration rate of microorganisms on solid culture dishes with high measurement accuracy.
Disclosure of Invention
The utility model aims to provide a device for measuring the microorganism respiration rate on a solid culture dish, which solves the problems in the prior art, utilizes a cover body to be connected with a groove from the inside, the cover body occupies the space in the groove, and utilizes the specification of the solid culture dish to design the size of the placing space after the cover body is connected with the groove, so that the volume of the gas which can be contained in the groove can be reduced, the internal gas quantity is reduced, and the final measurement accuracy is further improved.
In order to achieve the above object, the present utility model provides the following solutions: the utility model provides a measuring device for microorganism respiration rate on a solid culture dish, which comprises a bearing box and a gas analyzer for detecting the content of gas components, wherein the bearing box comprises a base and a cover body, a cylindrical groove is formed in the base, an internal thread is arranged on the inner wall surface of the groove, a cylindrical connecting part is arranged on the cover body, an external thread matched with the internal thread is arranged on the peripheral wall of the connecting part, the connecting part is in threaded connection with the groove, a placing space is formed between the inner bottom wall of the groove and the bottom end of the connecting part, the diameter of the placing space is not smaller than the diameter of the solid culture dish, the thickness of the placing space is not smaller than the thickness of the solid culture dish, and a sampling needle head of the gas analyzer is positioned in the placing space.
Preferably, the cover body is provided with a connecting hole penetrating through the cover body up and down, the connecting hole is internally connected with a stud with hollow inside in a threaded manner, the top of the stud is connected with a nut in a threaded manner, the position of the nut corresponding to the central hole of the stud is provided with a through hole, a rubber gasket is arranged between the inner bottom wall of the nut and the top end of the stud, the diameter of the rubber gasket is larger than that of the through hole, and the sampling needle head penetrates through the rubber gasket and extends into the placing space.
Preferably, the diameter of the rubber gasket is the same as the diameter of the stud.
Preferably, the middle part of the stud is in threaded connection with a nut.
Preferably, the connection length of the stud and the connection hole is smaller than the length of the connection hole.
Preferably, the cover body comprises a cover plate and a connecting part, the diameter of the cover plate is larger than that of the groove, an annular groove is formed in the periphery of the opening at the top of the groove, a rubber ring is embedded in the annular groove, and the diameter of the rubber ring is smaller than that of the cover plate.
Preferably, the base is cylindrical, and the outer diameter of the base is the same as the outer diameter of the cover plate.
Preferably, the position on the inner peripheral wall of the groove corresponding to the placing space is provided with an annular bulge, the inner diameter of the annular bulge is not smaller than the diameter of the solid culture dish, the internal thread is arranged above the annular bulge, and the distance between the upper surface of the annular bulge and the inner bottom wall of the groove is matched with the thickness of the solid culture dish.
Preferably, the cover body is made of transparent organic glass.
Compared with the prior art, the utility model has the following technical effects:
1. the cover body extends into the groove to be connected with the groove, so that the cover body occupies space in the groove, the volume of the gas which can be contained in the groove after connection is reduced, the size of the placement space formed by the cover body and the groove after connection is designed based on the specification of the solid culture dish, the volume of the gas which can be contained in the groove can be further reduced on the basis of ensuring that the solid culture dish can be placed in the groove, the smaller the volume of the gas which can be contained in the groove is, the smaller the gas quantity in the groove is, and the more accurate analysis and measurement of the content change of the gas in the gas analyzer are facilitated.
2. The hollow stud and the rubber gasket are arranged, so that the sampling needle head of the gas analyzer can be inserted into the placing space under the condition of ensuring sealing, and the convenience and the sealing performance of use are improved.
3. According to the utility model, the cover plate is continuously pressed down in the process of connecting the connecting part and the groove, so that the rubber ring is pressed tightly, the rubber ring is deformed, and the tightness between the cover plate and the upper surface of the base can be improved.
Drawings
In order to more clearly illustrate the embodiments of the present utility model or the technical solutions of the prior art, the drawings that are needed in the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present utility model, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is an exploded view of a device for measuring the respiration rate of microorganisms on a solid culture dish according to the present utility model;
FIG. 2 is a schematic view of the structure of the base of the present utility model;
FIG. 3 is a schematic view of the structure of the cover of the present utility model;
FIG. 4 is a schematic view of the structure of the bottom of the cover of the present utility model;
wherein, 1, bearing box; 2. a base; 3. a groove; 4. an internal thread; 5. a connection part; 6. an external thread; 7. placing space; 8. a connection hole; 9. a stud; 10. a screw cap; 11. a through hole; 12. a rubber gasket; 13. a nut; 14. an annular groove; 15. a rubber ring; 16. an annular protrusion; 17. and a cover plate.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
The utility model aims to provide a device for measuring the microorganism respiration rate on a solid culture dish, which solves the problems in the prior art, utilizes a cover body to be connected with a groove from the inside, the cover body occupies the space in the groove, and utilizes the specification of the solid culture dish to design the size of the placing space after the cover body is connected with the groove, so that the volume of the gas which can be contained in the groove can be reduced, the internal gas quantity is reduced, and the final measurement accuracy is further improved.
In order that the above-recited objects, features and advantages of the present utility model will become more readily apparent, a more particular description of the utility model will be rendered by reference to the appended drawings and appended detailed description.
Referring to fig. 1-4, a measuring device for microorganism respiration rate on a solid culture dish is provided, including bearing box 1 and a gas analyzer (not shown in the figure) for detecting gas component content, bearing box 1 includes a base 2 and a cover body, a cylindrical groove 3 is provided on the base 2, an internal thread 4 is provided on the inner wall surface of groove 3, a cylindrical connecting portion 5 is provided on the cover body, an external thread 6 matched with internal thread 4 is provided on the outer peripheral wall of connecting portion 5, connecting portion 5 is in threaded connection with groove 3, a placing space 7 is provided between the bottom wall in groove 3 and the bottom end of connecting portion 5, the diameter of placing space 7 is not less than the diameter of the solid culture dish, the thickness of placing space 7 is not less than the thickness of the solid culture dish, preferably, the thickness of placing space 7 is the same as the thickness of the solid culture dish, the diameter of placing space 7 is 1mm larger than the diameter of the solid culture dish, the purpose is to leave a certain gap to improve convenience of taking out the solid culture dish, the sampling needle of the gas analyzer is located in the placing space 7, the working principle: the mode that the lid stretches into in the recess 3 and is connected with the recess 3 makes the lid occupy the space in the recess 3, can hold gaseous volume in the recess 3 after the reduction is connected, and the lid is connected with the recess 3 after the space 7 size that places that forms is based on the specification of solid culture dish in addition removes the design, can guarantee on the basis that solid culture dish can put into, further reduce can hold gaseous volume in the recess 3, can hold gaseous volume and be less more, the tolerance is less more in the recess 3, is favorable to the analysis that gas analyzer is more accurate to measure inside gas composition content and changes more.
The sampling needle head of the gas analyzer can be fixedly arranged on the cover body or can be detachably arranged on the cover body, when the sampling needle head is fixedly arranged, a hole matched with the sampling needle is directly formed in the cover body, the sampling needle head is fixed by utilizing sealant, when the sampling needle head is detachably arranged, a connecting hole 8 penetrating up and down is formed in the cover body, an internal thread 4 of the connecting hole 8 is connected with an internal hollow stud 9, a screw cap 10 is connected with the top of the stud 9 in a threaded manner, a through hole 11 is formed in the position of the screw cap 10 corresponding to the central hole of the stud 9, a rubber gasket 12 is arranged between the top end of the stud 9 and the inner bottom wall of the screw cap 10, the diameter of the rubber gasket 12 is larger than that of the through hole 11, the sampling needle head penetrates through the rubber gasket 12 to extend into the placing space 7, and the hollow stud 9 and the sampling needle head of the rubber gasket 12 can be inserted into the placing space 7 under the condition of ensuring sealing, so that convenience and sealing of use are improved.
The nut 10 may be a conventional nut and a washer having a through hole 11 may be glued or welded to one end of the nut.
In order to avoid the problem of sealing failure caused by misalignment of the rubber gasket 12 between the nut 10 and the stud 9, the diameter of the rubber gasket 12 is set to be the same as the diameter of the stud 9.
The middle part threaded connection of double-screw bolt 9 has nut 13, and like this when double-screw bolt 9 screw in connecting hole 8, the bottom surface of nut 13 can contact with the lid upper surface, improves the area of contact between double-screw bolt 9 and nut 13 and connecting hole 8, improves the leakproofness.
A sealing ring can be arranged on the upper surface of the cover body corresponding to the lower surface of the nut 13, so that the tightness is further improved.
The connection length of the stud 9 and the connection hole 8 is smaller than the length of the connection hole 8, so that the cost is saved.
The lid includes apron 17 and connecting portion 5, apron 17 is disc structure, and apron 17 and connecting portion 5 coaxial setting, the diameter of apron 17 is greater than the diameter of recess 3, be provided with ring channel 14 around the opening of recess 3 top, the embedded rubber circle 15 that is equipped with of ring channel 14, the diameter of rubber circle 15 is less than the diameter of apron 17, the notch of outstanding ring channel 14 when not extrudeing the state of rubber circle 15, apron 17 can constantly push down at connecting portion 5 and recess 3 in-process to compress tightly rubber circle 15, make rubber circle 15 warp, and then can improve the leakproofness between apron 17 and the base 2 upper surface.
The cover plate 17 and the connecting part 5 are integrally arranged, so that the structural strength is improved.
The base 2 is cylindrical, and the outer diameter of the base 2 is the same as the outer diameter of the cover plate 17.
The position of placing space 7 on the recess 3 inner peripheral wall corresponds is provided with annular protruding 16, and the internal diameter of annular protruding 16 is not less than the diameter of solid culture dish, and space 7 can be placed to the space that annular protruding 16 encircleed and formed, and internal thread 4 sets up in annular protruding 16 top, and the distance between annular protruding 16 upper surface and the bottom wall in recess 3 matches with the thickness phase-match of solid culture dish, and annular protruding 16 can play spacing effect, prevents that connecting portion 5 transition from stretching into the extrusion solid culture dish, causes the damage of solid culture dish.
The annular bulge 16 and the base 2 are integrally formed, so that the structural strength is improved.
In order to facilitate the observation of the condition of the solid culture dish in the base, the cover plate 17 and the cover body formed by the connecting part 5 are made of transparent organic glass, and workers can directly observe the inside through the cover body.
In the actual use process, 75% alcohol is firstly used for disinfecting the base 2, the groove 3, the cover body, the stud 9 and the screw cap 10, then a solid culture dish filled with microorganisms is placed in the placing space 7, the cover body is screwed downwards, and a sampling needle head of the gas analyzer extends into the placing space 7 to obtain the change of the content of the internal gas components.
The adaptation to the actual need is within the scope of the utility model.
It should be noted that it will be apparent to those skilled in the art that the present utility model is not limited to the details of the above-described exemplary embodiments, but may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
The principles and embodiments of the present utility model have been described in detail with reference to specific examples, which are provided to facilitate understanding of the method and core ideas of the present utility model; also, it is within the scope of the present utility model to be modified by those of ordinary skill in the art in light of the present teachings. In view of the foregoing, this description should not be construed as limiting the utility model.
Claims (9)
1. The utility model provides a measuring device of microorganism respiration rate on solid culture dish, its characterized in that, including bearing box and being used for detecting gaseous composition content's gas analysis appearance, bear box and include base and lid, set up on the base and be columniform recess, be provided with the internal thread on the internal face of recess, be provided with on the lid and be columniform connecting portion, be provided with on the connecting portion periphery wall with internal thread assorted external screw thread, connecting portion with recess threaded connection, the recess diapire with be placed space between the connecting portion bottom, place the diameter of space and be not less than the diameter of solid culture dish, place the thickness of space and be not less than the thickness of solid culture dish, gas analysis appearance's sampling needle is located place the space.
2. The device for measuring the respiration rate of microorganisms on the solid culture dish according to claim 1, wherein the cover body is provided with a connecting hole penetrating up and down, a stud with a hollow inside is connected in the connecting hole in a threaded manner, a nut is connected to the top of the stud in a threaded manner, a through hole is arranged at the position of the nut corresponding to the central hole of the stud, a rubber gasket is arranged between the inner bottom wall of the nut and the top end of the stud, the diameter of the rubber gasket is larger than that of the through hole, and the sampling needle head penetrates through the rubber gasket and extends into the placing space.
3. The device for measuring the respiration rate of microorganisms on a solid culture dish according to claim 2, wherein the diameter of the rubber gasket is the same as the diameter of the stud.
4. The device for measuring the respiration rate of microorganisms on a solid culture dish according to claim 2, wherein a nut is screwed in the middle of the stud.
5. The device for measuring the respiration rate of microorganisms on a solid culture dish according to claim 4, wherein the connection length of the stud and the connection hole is smaller than the connection hole.
6. The device for measuring the respiration rate of microorganisms on a solid culture dish according to claim 1, wherein the cover body comprises a cover plate and the connecting part, the diameter of the cover plate is larger than that of the groove, an annular groove is formed around the opening at the top of the groove, a rubber ring is embedded in the annular groove, and the diameter of the rubber ring is smaller than that of the cover plate.
7. The device for measuring the respiration rate of microorganisms on a solid culture dish according to claim 6, wherein the base is cylindrical and has an outer diameter identical to that of the cover plate.
8. The device for measuring the respiration rate of microorganisms on a solid culture dish according to claim 1, wherein an annular protrusion is arranged on the inner peripheral wall of the groove at a position corresponding to the placing space, the inner diameter of the annular protrusion is not smaller than the diameter of the solid culture dish, the internal thread is arranged above the annular protrusion, and the distance between the upper surface of the annular protrusion and the inner bottom wall of the groove is matched with the thickness of the solid culture dish.
9. The device for measuring the respiration rate of microorganisms on a solid culture dish according to claim 1, wherein the cover is made of transparent organic glass.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322081780.4U CN220564585U (en) | 2023-08-03 | 2023-08-03 | Measuring device for microorganism respiration rate on solid culture dish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322081780.4U CN220564585U (en) | 2023-08-03 | 2023-08-03 | Measuring device for microorganism respiration rate on solid culture dish |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220564585U true CN220564585U (en) | 2024-03-08 |
Family
ID=90092193
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322081780.4U Active CN220564585U (en) | 2023-08-03 | 2023-08-03 | Measuring device for microorganism respiration rate on solid culture dish |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220564585U (en) |
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2023
- 2023-08-03 CN CN202322081780.4U patent/CN220564585U/en active Active
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