CN112166903A - Plant carbon isotope labeling device and plant carbon isotope labeling method - Google Patents
Plant carbon isotope labeling device and plant carbon isotope labeling method Download PDFInfo
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- CN112166903A CN112166903A CN202011122877.XA CN202011122877A CN112166903A CN 112166903 A CN112166903 A CN 112166903A CN 202011122877 A CN202011122877 A CN 202011122877A CN 112166903 A CN112166903 A CN 112166903A
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 60
- 229910052799 carbon Inorganic materials 0.000 title claims abstract description 60
- 238000001948 isotopic labelling Methods 0.000 title claims abstract description 22
- 238000003141 isotope labeling method Methods 0.000 title claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 39
- 238000007791 dehumidification Methods 0.000 claims abstract description 22
- 238000000034 method Methods 0.000 claims abstract description 9
- 238000002156 mixing Methods 0.000 claims abstract description 9
- 238000007789 sealing Methods 0.000 claims abstract description 8
- 238000000265 homogenisation Methods 0.000 claims abstract 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 10
- 238000012360 testing method Methods 0.000 claims description 10
- 239000002689 soil Substances 0.000 claims description 7
- 238000002347 injection Methods 0.000 claims description 6
- 239000007924 injection Substances 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- 230000008569 process Effects 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 4
- 238000005303 weighing Methods 0.000 claims description 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 3
- 239000001110 calcium chloride Substances 0.000 claims description 3
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 3
- 238000011049 filling Methods 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 2
- 239000000470 constituent Substances 0.000 claims 1
- 230000005611 electricity Effects 0.000 abstract description 5
- 241000196324 Embryophyta Species 0.000 description 34
- 230000000243 photosynthetic effect Effects 0.000 description 7
- 229920000742 Cotton Polymers 0.000 description 6
- 238000011160 research Methods 0.000 description 6
- 241000219146 Gossypium Species 0.000 description 5
- 238000009826 distribution Methods 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000000700 radioactive tracer Substances 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 229910000838 Al alloy Inorganic materials 0.000 description 1
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000005755 formation reaction Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000000155 isotopic effect Effects 0.000 description 1
- 229910052744 lithium Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000013076 target substance Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/14—Greenhouses
- A01G9/16—Dismountable or portable greenhouses ; Greenhouses with sliding roofs
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/18—Greenhouses for treating plants with carbon dioxide or the like
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/24—Devices or systems for heating, ventilating, regulating temperature, illuminating, or watering, in greenhouses, forcing-frames, or the like
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
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- Pathology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention provides a plant carbon isotope labeling device and a plant carbon isotope labeling method, which not only can ensure the sealing performance of a box body and simultaneously remarkably simplify the structure of the box body and a tracing procedure, but also solve the problem of using water and electricity at the field. The device comprises a homogenization tank, a gas-mixing temperature and humidity adjusting structure, a dehumidification bag, a reactor and a communicating pipe; the assimilation box comprises an upper box body and a lower box body; the lower box body is a frame body with the upper end and the lower end open and the other surfaces closed, and the upper end surface of the lower box body is provided with a clip-shaped water tank; the upper box body is a frame body with an open lower end and closed other surfaces, and is arranged on the square-shaped water tank; the air-mixing temperature and humidity adjusting structure comprises a fan I and a fan II which are arranged in an assimilation box in a diagonal manner; the dehumidification bag is arranged in the middle of the assimilation box; the reactor is positioned in the lower box body and is arranged on the ground; one end of the communicating pipe is arranged in the reactor, and the other end of the communicating pipe passes through the upper box body and is communicated with the outside.
Description
Technical Field
The invention relates to a plant carbon isotope labeling device and a plant carbon isotope labeling method, and belongs to the technical field of isotope tracing.
Background
The isotope labeling technology utilizes radioactive or stable isotope labeling agents to research the laws of migration, transformation, transportation, absorption, metabolism and the like of target substances in soil, animals and plants and in the environment, has strong specificity, specificity and traceability, and is widely applied to agricultural science research. The carbon isotope tracer technique is widely applied to research on crop photosynthetic carbon distribution and transportation and soil carbon conversion and circulation, and particularly can provide information on the accumulation, distribution and conversion of photosynthetic carbon in different growth periods of crops, dynamically monitor the destination of the photosynthetic carbon and provide complete production and distribution conditions of photosynthetic substances of the crops. At present, researchers create various isotopic tracing devices and tracing methods by using potted plants or small plants, and obtain good tracing effects.
In recent years, along with the enhancement of agricultural scientists on research and exploration desire of real crops growing in field environment, the existing carbon isotope tracer devices are single in specification, complex in structure, heavy in machine type, not easy to move, and difficult to meet field conditions of limited operation time and labor and no water and no electricity due to the fact that water and electricity are required to be supplied continuously. Therefore, how to further improve the isotope tracing efficiency, simplify the carbon isotope tracing device, reduce the weight of the machine body, and save water and supply power is a problem which needs to be solved at present.
Disclosure of Invention
Aiming at the defects in the existing device, the invention aims to provide the plant carbon isotope labeling device and the plant carbon isotope labeling method, different using modes can be customized and selected according to different crops or different growth periods of the same crop, the box structure and the tracing process can be obviously simplified while the box body sealing performance is ensured, the problem of using water and electricity at the field and the land is solved, and the tracing efficiency is further improved.
In order to achieve the purpose, the invention is realized by the following technical scheme:
a plant carbon isotope labeling device comprises an assimilation box, a gas-mixing temperature and humidity adjusting structure, a dehumidification bag, a reactor and a communicating pipe;
the assimilation box comprises an upper box body and a lower box body; the lower box body is a frame body with the upper end and the lower end open and the other surfaces closed, and the upper end surface of the lower box body is provided with a clip-shaped water tank; the upper box body is a frame body with an open lower end and closed other surfaces, and is arranged on the square-shaped water tank (3);
the air-mixing temperature and humidity adjusting structure comprises a fan I and a fan II which are arranged in an assimilation box in a diagonal manner;
the dehumidification bag is arranged in the middle of the assimilation box;
the reactor is positioned in the lower box body and is arranged on the ground;
one end of the communicating pipe is arranged in the reactor, and the other end of the communicating pipe passes through the upper box body and is communicated with the outside.
According to the preferable scheme of the plant carbon isotope labeling device, a harness is arranged on the end face of the right lower corner of the front face of the upper box body, and a plurality of pipe holes are reserved in the harness.
According to the preferable scheme of the plant carbon isotope labeling device, a fan I is hung on a first supporting rod and is positioned in an upper box body, the height of the first supporting rod is consistent with that of a plant, and a fan II is arranged on the ground and is positioned in a lower box body.
According to the preferable scheme of the plant carbon isotope labeling device, the dehumidification bag contains spherical calcium chloride particles and is hung on a second support rod, and the height of the second support rod is equal to half of the height of the assimilation box.
In the preferable scheme of the plant carbon isotope labeling device, a pair of operating handles are respectively arranged on the outer side wall surfaces of the upper box body and the lower box body.
A plant carbon isotope labeling method comprises the following steps:
s1, selecting continuous and uniformly good-growing plants in a field, marking the plants by hanging tags, and sleeving the plants to be traced into a lower box body; after the lower box body is laid flat, the soil is tightly sealed and compacted, and the joint of the lower box body and the ground is ensured to be airtight;
s2, placing the fan I, the fan II, the dehumidification bag and the reactor, placing one end of the communicating pipe in the reactor, and placing the other end of the communicating pipe on the upper edge of the lower box body for later use;
s3, pouring the weighed carbon isotope preparation into a reactor, buckling the upper box body on a square-shaped water tank of the lower box body, penetrating a communicating pipe through the pipe hole in the lowering process of the upper box body, and then filling water into the square-shaped water tank to form a closed carbon isotope tracing assimilation box;
s4, checking the sealing performance of the assimilation box, and injecting excessive hydrochloric acid into the reactor through a communicating pipe after the assimilation box is completely sealed to generate13CO2, plugging the communicating pipe after the injection is finished;
s5, after the carbon isotope tracing is finished, communicating a pipe, lifting the upper box body out of the field, closing the fan I and the fan II, taking out the fan I, the fan II, the dehumidification bag and the reactor, slightly lifting one corner of the lower box body to pour out water in the square-shaped water tank, and lifting the lower box body out of the field to finish the carbon isotope tracing;
s6, digging out plants with roots, dividing samples according to test requirements, drying and weighing, and measuring the plant components by using an isotope mass spectrometer13And (4) C content.
The invention has the advantages that:
1. the plant carbon isotope labeling device simplifies the devices such as a condensation and ventilation pipeline, a motor and the like in the prior art, not only obviously reduces the weight of the machine body, but also effectively reduces the frequency of punching the side surface of the box body and ensures the airtightness and integrity of the assimilation box body.
2. The lower box body can adjust the depth of the lower box body buried in soil according to the plant height of crops, and the appropriate height of the assimilation box is determined, so that not only is the waste of carbon isotope preparations prevented, but also the use frequency of the box body is remarkably improved.
3. The fan and the dehumidifying bag effectively reduce the temperature and the humidity in the box body, greatly improve the tracing efficiency and solve the problem of field carbon isotope tracing related research.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention.
FIG. 1 is a schematic structural view of a carbon isotope labeling apparatus for plants according to the present invention;
in the figure, 1-an upper box body, 2-a lower box body, 3-a square-shaped water tank, 4-pipe holes, 5-fans I, 6-a dehumidification bag, 7-a communicating pipe, 8-a reactor, 9-fans II, 10-an operating handle.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
A plant carbon isotope labeling device comprises an assimilation box, a gas-mixing temperature and humidity adjusting structure, a dehumidification bag, a reactor and a communicating pipe 7;
the assimilation box comprises an upper box body 1 and a lower box body 2; the lower box body 2 is a frame body with the upper end and the lower end open and other surfaces closed, and the upper end surface of the lower box body 2 is provided with a clip-shaped water tank 3; the upper box body 1 is a frame body with an open lower end and closed other surfaces, and the upper box body 1 is arranged on the square-shaped water tank 3;
the gas mixing and temperature and humidity adjusting structure comprises a fan I9 and a fan II 5 which are arranged in the assimilation box in opposite angles and used for uniformly mixing gas in the assimilation box and simultaneously reducing the temperature and humidity of the box body;
the dehumidification bag 6 is arranged in the middle of the assimilation box;
the reactor 8 is positioned on the lower box body 2 and is arranged on the ground;
one end of the communicating pipe 7 is arranged in the reactor 8, and the other end passes through the upper box body 1 and is communicated with the outside.
The end face of the right lower corner of the front surface of the upper box body 1 is provided with a harness, and a plurality of pipe holes 4 are reserved on the harness.
The fan I9 is hung on the first supporting rod and located in the upper box body 1, the height of the first supporting rod is consistent with that of the plants, and the fan II 5 is arranged on the ground and located in the lower box body 2.
In this embodiment, dehumidification bag 6 contains ball-type calcium chloride granule, and hangs on the second bracing piece, and the second bracing piece height equals the half of assimilation box height for absorb airtight box internal vapor, further reduce the humidity of box internal environment.
For ease of handling and movement, the outer side walls of the upper and lower cases 1 and 2 are respectively provided with a pair of handling grips 10.
A plant carbon isotope labeling method comprises the following steps:
s1, selecting continuous and uniformly well-grown plants in a field, marking the plants by hanging tags, and sleeving the plants to be traced into a lower box body 2; after the lower box body 2 is laid flat, the soil is tightly sealed and compacted, and the joint of the lower box body 2 and the ground is ensured to be airtight;
s2, placing the fan I9, the fan II 5, the dehumidification bag 6 and the reactor 8, placing one end of the communicating pipe 7 in the reactor 8, and placing the other end of the communicating pipe on the upper edge of the lower box body 2 for later use;
s3, pouring the weighed carbon isotope preparation into a reactor, buckling the upper box body 1 on a square-shaped water tank 3 of the lower box body 2, enabling a communicating pipe 7 to penetrate through the pipe hole 4 in the lowering process of the upper box body 1, and then filling water into the square-shaped water tank 3 to form a closed carbon isotope tracing assimilation box;
s4, checking the sealing performance of the assimilation box, injecting excessive hydrochloric acid into a reactor 8 through a communicating pipe 7 after the assimilation box is completely sealed to generate13CO2, and the communicating pipe 7 is sealed after the injection is finished;
s5, after the carbon isotope tracing is finished, lifting the upper box body 1 out of the field through a communicating pipe 7, closing a fan I9 and a fan II 5, taking out the fan I9, the fan II 5, the dehumidification bag 6 and the reactor 8, slightly lifting one corner of the lower box body 2 to pour out water in the square-shaped water tank 3, and lifting the lower box body out of the field to finish the carbon isotope tracing;
s6, digging out plants with roots, dividing samples according to test requirements, drying and weighing, and measuring the plant components by using an isotope mass spectrometer13And (4) C content.
When the carbon isotope labeling device is used for carrying out crop carbon isotope labeling related tests, the proper size of the assimilation box is calculated and customized in advance according to crop growth and development characteristics and a plant row spacing configuration mode.
The invention should now be seenThe assimilation box of the technology is described in detail in a case of research on the law of photosynthetic carbon accumulation and distribution in cotton boll stage, and the size of the assimilation box customized by the case is as follows: the upper box body 1 is 60cm long, 75cm wide and 100cm high, the lower box body 2 is 60cm long, 75cm wide and 40cm high, the square-shaped water tank 3 is arranged at the upper edge of the lower box body 2 and is welded into a water tank with 6cm wide and 9cm high, the heights of the first supporting rod and the second supporting rod are 100cm and 70cm respectively and are used for hanging the fan I9 and the dehumidifying bag 6 respectively; the reactor is a glass beaker special for test, is used for the reaction of carbon isotope preparation and dilute hydrochloric acid, and has the specification of more than 2L as much as possible so as to ensure the built-in Ba13CO3Fully reacts with HCL and does not splash.
Before the test is started, the weather condition needs to be observed, and the weather is clear, 9: 00-15% in the afternoon on a certain day without wind or with weak wind: and 00, avoiding noon time, and preventing the influence of the carbon tracing effect on crops due to the noon break phenomenon. In the afternoon of the day before the carbon tracing test, 3-4 continuous and uniformly well-grown cottons are selected in the field, marked by hanging tags, and after the ground near the field is leveled by a shovel, 2 persons cooperate to sleeve the cottons to be traced into the lower box body 2 of the invention, during the period, the distance between the edge of the box body and cotton branches and leaves is noticed, and the box body 2 is slowly put down on the ground to prevent the cotton branches and leaves from colliding; after the lower box body 2 is laid flat, a shovel is used for digging a little soil to seal the lower wall of the periphery of the lower box body 2 by 5-10 cm, and the lower box body is compacted by stepping on the shovel, so that the joint of the lower box body 2 and the ground is ensured to be airtight.
1 hour before the carbon tracer test starts, a fan I9, a fan II 5, a dehumidification bag 6 and a reactor 8 are placed in a lower box body: the fan I9 and the fan II 5 are respectively placed at diagonal positions of the box body, wherein the fan II 5 is directly placed on the ground, and the fan I9 is hung on a first supporting rod with the same height as crops; the dehumidification bag 6 is hung on a second supporting rod with half height and length of the crops and is inserted into the middle position of the ground inside the lower box body; the reactor 8 is placed on the ground beside the second support rod, one end of the communicating pipe is placed in the reactor, and the other end is placed on the upper edge of the lower box body for standby.
Before the test, the position of each component of the device and the integrity thereof are checked, and after all the components are ready, the fan is turned on, and the weighed carbon isotope preparation (Ba) is added13CO3The dosage of the photosynthetic rate is estimated by the photosynthetic rate of a certain crop group and the tracing time) is poured into the reactor, and 2 persons cooperate to slowly put down the upper box body and buckle the upper box body in a circular water tank of the lower box body. And in the process of lowering the upper box body, the communicating pipe arranged on the upper edge of the lower box body penetrates through the pipe hole 4, and then the water tank 3 is filled with water, so that the closed carbon isotope tracing assimilation box is formed.
Checking the sealing property of the assimilation box, and adding hydrochloric acid to generate the product after the sealing is complete13 CO2. Excess dilute hydrochloric acid is injected into the reactor through a communicating pipe 7 for ensuring13CO2The formation reaction is sufficient, and a small amount of injection for multiple times is recommended. During the process, the dovetail clamp is used for clamping the communicating pipe after each injection is finished, so that gas leakage is prevented.
And (3) after the carbon isotope tracing is finished, taking down the dovetail clamp, drawing out the communicating pipe, lifting the upper box body 1 out of the field by 2 people in cooperation, closing the fan, taking the fan, the dehumidification bag and the reactor out of the box body, slightly lifting one corner of the box body 2 to pour out water in the square-shaped water tank 3, and then lifting the lower box body 2 out of the field by 2 people in cooperation, thus finishing the carbon isotope tracing. Digging out cotton with root, dividing according to test requirements, drying and weighing, and measuring plant components with isotope mass spectrometer13And (4) C content.
In the technical scheme, the upper box body is manufactured by 12 aluminum alloy square tubes and the greenhouse non-drop plastic film, and the lower box body is welded by 4 rectangular iron plates with the thickness of 0.03cm, so that the weight of the carbon isotope tracing assimilation box is obviously reduced, and the mobility and the operability are improved; the use of the lithium battery fan and the finished product dehumidification bag not only reduces the temperature and humidity in the assimilation box, but also continuously supplies air and uniformly mixes13CO2 gas, and solves the problem of the condensate water for farmland electricity; the water injection sealing of the lower box body and the square-shaped water tank obviously improves the airtightness of the assimilation box and improves the accuracy and reliability of carbon marking.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (6)
1. A plant carbon isotope labeling device is characterized in that: comprises a homogenization box, a gas-mixing temperature and humidity adjusting structure, a dehumidification bag, a reactor and a communicating pipe (7);
the assimilation box comprises an upper box body (1) and a lower box body (2); the lower box body (2) is a frame body with the upper end and the lower end open and other surfaces closed, and the upper end surface of the lower box body (2) is provided with a clip-shaped water tank (3); the upper box body (1) is a frame body with an open lower end and closed other surfaces, and the upper box body (1) is arranged on the square-shaped water tank (3);
the air-mixing temperature and humidity adjusting structure comprises a fan I (9) and a fan II (5) which are arranged in the assimilation box in a diagonal manner;
the dehumidification bag (6) is arranged in the middle of the assimilation box;
the reactor (8) is positioned on the lower box body (2) and is arranged on the ground;
one end of the communicating pipe (7) is arranged in the reactor (8), and the other end passes through the upper box body (1) to be communicated with the outside.
2. The carbon isotope labeling apparatus for plants according to claim 1, wherein: the end face of the right lower corner of the front surface of the upper box body (1) is provided with a harness, and a plurality of pipe holes (4) are reserved on the harness.
3. The carbon isotope labeling apparatus for plants according to claim 1, wherein: the fan I (9) is hung on the first supporting rod and is positioned in the upper box body (1), the height of the first supporting rod is consistent with that of the plants, and the fan II (5) is arranged on the ground and is positioned in the lower box body (2).
4. The carbon isotope labeling apparatus for plants according to claim 1, wherein: the dehumidification bag (6) contains spherical calcium chloride particles and is hung on a second support rod, and the height of the second support rod is equal to half of the height of the assimilation box.
5. The carbon isotope labeling apparatus for plants according to any one of claims 1 to 4, wherein: the outer side wall surfaces of the upper box body (1) and the lower box body (2) are respectively provided with a pair of operating handles (10).
6. A plant carbon isotope labeling method is characterized by comprising the following steps:
s1, selecting continuous and uniformly well-grown plants in a field, marking the plants by hanging tags, and sleeving the plants to be traced into a lower box body (2); after the lower box body (2) is laid flat, the soil is tightly sealed and compacted, and the joint of the lower box body (2) and the ground is ensured to be airtight;
s2, placing the fan I (9), the fan II (5), the dehumidification bag (6) and the reactor (8), placing one end of the communicating pipe (7) in the reactor (8), and placing the other end of the communicating pipe at the upper edge of the lower box body (2) for later use;
s3, pouring the weighed carbon isotope preparation into a reactor, buckling the upper box body (1) on a square-shaped water tank (3) of the lower box body (2), enabling a communicating pipe (7) to penetrate through the pipe hole (4) in the downward-placing process of the upper box body (1), and then filling water into the square-shaped water tank (3) to form a closed carbon isotope tracing assimilation box;
s4, checking the sealing performance of the assimilation box, injecting excessive hydrochloric acid into the reactor (8) through a communicating pipe (7) after the assimilation box is sealed completely to generate13CO2, plugging the communicating pipe (7) after the injection is finished;
s5, after the carbon isotope tracing is finished, lifting the upper box body (1) out of the field through a communicating pipe (7), closing the fan I (9) and the fan II (5), taking the fan I (9), the fan II (5), the dehumidification bag (6) and the reactor (8) out, slightly lifting one corner of the box body (2) to pour out water in the square-shaped water tank (3), and lifting the lower box body out of the field to finish the carbon isotope tracing;
s6, digging out plants with roots, dividing samples according to test requirements, drying and weighing, and measuring by using an isotope mass spectrometerOf the constituents of the plants13And (4) C content.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011122877.XA CN112166903A (en) | 2020-10-20 | 2020-10-20 | Plant carbon isotope labeling device and plant carbon isotope labeling method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011122877.XA CN112166903A (en) | 2020-10-20 | 2020-10-20 | Plant carbon isotope labeling device and plant carbon isotope labeling method |
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| Publication Number | Publication Date |
|---|---|
| CN112166903A true CN112166903A (en) | 2021-01-05 |
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| CN202011122877.XA Pending CN112166903A (en) | 2020-10-20 | 2020-10-20 | Plant carbon isotope labeling device and plant carbon isotope labeling method |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102252874A (en) * | 2011-04-03 | 2011-11-23 | 兰州大学 | Gas collection device |
| CN204269629U (en) * | 2014-11-28 | 2015-04-15 | 中国农业大学 | A kind of carbon isotope labelling device |
| US20150309038A1 (en) * | 2012-11-21 | 2015-10-29 | Basf Plant Science Company Gmbh | Method and device for marking isotopes |
| CN204903503U (en) * | 2015-08-21 | 2015-12-23 | 中国科学院南京土壤研究所 | Carbon isotope mark device |
| CN209417022U (en) * | 2019-01-11 | 2019-09-20 | 四川省农业科学院土壤肥料研究所 | A kind of carbon isotope labelling device for various plants |
| CN213486036U (en) * | 2020-10-20 | 2021-06-22 | 山东棉花研究中心 | Plant carbon isotope labeling device |
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- 2020-10-20 CN CN202011122877.XA patent/CN112166903A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102252874A (en) * | 2011-04-03 | 2011-11-23 | 兰州大学 | Gas collection device |
| US20150309038A1 (en) * | 2012-11-21 | 2015-10-29 | Basf Plant Science Company Gmbh | Method and device for marking isotopes |
| CN204269629U (en) * | 2014-11-28 | 2015-04-15 | 中国农业大学 | A kind of carbon isotope labelling device |
| CN204903503U (en) * | 2015-08-21 | 2015-12-23 | 中国科学院南京土壤研究所 | Carbon isotope mark device |
| CN209417022U (en) * | 2019-01-11 | 2019-09-20 | 四川省农业科学院土壤肥料研究所 | A kind of carbon isotope labelling device for various plants |
| CN213486036U (en) * | 2020-10-20 | 2021-06-22 | 山东棉花研究中心 | Plant carbon isotope labeling device |
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