CN112159750A - Culture dish tray - Google Patents

Culture dish tray Download PDF

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Publication number
CN112159750A
CN112159750A CN202011007906.8A CN202011007906A CN112159750A CN 112159750 A CN112159750 A CN 112159750A CN 202011007906 A CN202011007906 A CN 202011007906A CN 112159750 A CN112159750 A CN 112159750A
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CN
China
Prior art keywords
tray
dish
liquid
oil
dish box
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CN202011007906.8A
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Chinese (zh)
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云新
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Wuhan Huchuang United Technology Co ltd
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Wuhan Huchuang United Technology Co ltd
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Priority to CN202011007906.8A priority Critical patent/CN112159750A/en
Publication of CN112159750A publication Critical patent/CN112159750A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/44Multiple separable units; Modules
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports

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  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a culture dish tray which comprises a tray body, wherein the top surface of the tray body is provided with at least two first dish box placing positioning holes. The first dish box placing positioning hole is additionally arranged, so that the dish boxes (namely culture dishes) can be placed and positioned conveniently, a plurality of dish boxes can be operated simultaneously, and the efficiency is greatly improved. Is suitable for the preparation and pretreatment of a culture dish before embryo culture.

Description

Culture dish tray
Technical Field
The invention relates to the technical field of embryo culture, in particular to a culture dish tray.
Background
At present, in the field of assisted reproduction, a culture dish for embryo culture needs to be prepared in advance in the process of embryo culture, the process is called liquid preparation, and the operation process is to adopt an A burette to extract a certain amount of culture liquid; and a certain amount of mineral oil liquid is extracted by adopting a burette B; then, the burette A is squeezed to titrate the culture solution liquid in a culture dish according to a certain arrangement, the titration amount is balanced by adopting squeezing times, the titration position is manually controlled, and the titration size is manually controlled by observing through an operator; then, the burette B is squeezed to titrate a certain amount of mineral oil liquid, so that the mineral oil completely covers the liquid drop of the culture solution, and the change of osmotic pressure of the culture solution caused by volatilization of water in the culture solution is prevented; after dripping, manually putting the prepared culture dish into a CO2 incubator with constant temperature, constant humidity and certain constant concentration, enabling the temperature of the culture solution in the culture dish to rise to the temperature of the incubator, and adjusting the pH value of the culture solution to reach an optimal value through CO2 in the incubator. In the above operation, the culture dish is operated individually, which is inefficient and very labor-intensive.
Disclosure of Invention
The present invention is directed to a culture dish tray, which solves the above problems of the prior art.
In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a culture dish tray, includes tray body, tray body's top surface is equipped with two at least first wares box and places the locating hole. The first dish box placing positioning hole is additionally arranged, so that the dish boxes (namely culture dishes) can be placed and positioned conveniently, a plurality of dish boxes can be operated simultaneously, and the efficiency is greatly improved.
The invention provides a culture dish tray. The method has the following beneficial effects:
1. the additionally arranged first dish box placing positioning hole facilitates the placing and positioning of dish boxes (namely culture dishes), so that a plurality of dish boxes can be operated simultaneously, and the efficiency is greatly improved;
3. the additional stacking positioning support columns can realize multi-layer stacking of the trays, so that the floor area of the trays is saved, and the liquid preparation of a plurality of trays can be realized;
4. the additional clamping jaw grabbing grooves facilitate the clamping jaws to grab the tray smoothly;
5. the two-dimensional code can be marked in the additionally arranged two-dimensional code groove, so that the information of each tray can be marked;
6. the additional positioning guide holes facilitate the positioning of the tray;
7. the added lightening holes can effectively lighten the weight of the tray without influencing the strength and the use function of the tray;
8. through designing the dish box placing positioning holes with different diameters, the tray can be suitable for dish boxes with different models, so that the applicability of the tray is improved;
9. the number of the positioning guide holes is designed to be 6, and the 6 positioning guide holes are symmetrically arranged, so that the direction of the tray can not be divided when the tray is positioned;
10. the two-dimensional code in the two-dimensional code groove on the bottom surface of the tray body can quickly acquire the information of the tray upper dish box.
Drawings
FIG. 1 is a schematic perspective view of the liquid preparation instrument;
FIG. 2 is a schematic view of the structure of FIG. 1 from another perspective;
FIG. 3 is a schematic view of the structure of FIG. 1 from another perspective;
FIG. 4 is a schematic front view of the structure of FIG. 1;
FIG. 5 is a side view of the structure of FIG. 1;
FIG. 6 is a schematic top view of the structure of FIG. 1;
FIG. 7 is a schematic structural view of a liquid preparation mechanism;
FIG. 8 is a schematic view of the structure of FIG. 7 from another perspective;
FIG. 9 is a schematic front view of the structure of FIG. 7;
FIG. 10 is a side view of the structure of FIG. 7;
FIG. 11 is a schematic top view of the structure of FIG. 7;
FIG. 12 is a schematic structural view of a transfer mechanism;
FIG. 13 is a schematic view of the structure of FIG. 12 from another perspective;
FIG. 14 is a schematic view of the structure of FIG. 12 from yet another perspective;
FIG. 15 is a schematic front view of the structure of FIG. 12;
FIG. 16 is a side view of the structure of FIG. 12;
FIG. 17 is a bottom view of the structure of FIG. 12;
FIG. 18 is a schematic view of the structure of an incubator;
FIG. 19 is a schematic view of the structure of FIG. 18 from another perspective;
FIG. 20 is a front view of the structure of FIG. 18;
FIG. 21 is a side view of the structure of FIG. 18;
FIG. 22 is another side view schematic of the structure of FIG. 18;
FIG. 23 is a schematic top view of the structure of FIG. 18;
FIG. 24 is a schematic view showing the internal structure of the incubator;
FIG. 25 is a schematic view of the structure of FIG. 24 from another perspective;
FIG. 26 is a schematic view of the structure of FIG. 24 from a further angle of view;
figure 27 is a schematic diagram of the capsule moving assembly;
FIG. 28 is a schematic view of the structure of FIG. 27 from another perspective;
FIG. 29 is a schematic view of the oil bottle positioning assembly;
FIG. 30 is a schematic view of the structure of FIG. 29 from another perspective;
FIG. 31 is a schematic view of the structure of the tray;
FIG. 32 is a schematic view of the structure of FIG. 30 from another perspective;
FIG. 33 is a front view of the tray;
FIG. 34 is a schematic sectional view taken along line A-A of FIG. 33;
FIG. 35 is a schematic cross-sectional view taken along line B-B of FIG. 33;
fig. 36 is a schematic sectional view taken along line C-C in fig. 33.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1 to 36, the present invention provides a technical solution: the utility model provides an automatic prepare liquid appearance, includes liquid preparation mechanism 1, moves and carries mechanism 2 and incubator 3, liquid preparation mechanism 1, move and carry mechanism 2 and incubator 3 and all install on same bottom plate to form integrative structure, and liquid preparation mechanism 1 and incubator 3 are located and move the homonymy that moves mechanism 2. The liquid preparation mechanism 1 is used for completing liquid preparation operation to obtain a finished product, and the transfer mechanism 2 is used for placing the finished product into the incubator 3 for pretreatment. The liquid preparation mechanism 1 automatically completes the liquid preparation action, so that the position, the size and the shape of the titrated liquid drop are regular, the titrating amount of the liquid can be accurately controlled, and the oil covering amount of the liquid can also be accurately controlled; in addition, after the liquid preparation action is finished, the titrated liquid can be immediately placed into the incubator 3 for pretreatment by the transfer mechanism 2; finally, the whole liquid preparation process does not need manual intervention, thereby greatly reducing the labor cost.
Above-mentioned liquid mechanism 1 of preparing is including installing stand 1a on the bottom plate, install big crossbeam 1b on the stand 1a, big crossbeam 1b is gone up and is installed little crossbeam 1c to the removal along X, it installs dropping liquid subassembly 1d to the removal along Y on little crossbeam 1 c. And a small beam moving belt conveyor 1i for driving the small beam 1c to move is arranged on the large beam 1 b. Dropping liquid subassembly 1d includes dropping liquid buret 1d1, dropping liquid axle 1d2 and dropping liquid plunger pump 1d3, dropping liquid buret 1d1 upper end with dropping liquid axle 1d2 lower extreme links to each other, dropping liquid axle 1d2 upper end with dropping liquid plunger pump 1d3 links to each other, dropping liquid axle 1d2 is followed Z and is equipped with dropping liquid rack 1d4 along the axial on arranging and it, correspond on dropping liquid axle 1d2 dropping liquid rack 1d 4's position has dropping liquid slider 1d5 along axial slidable mounting, be equipped with on dropping liquid slider 1d5 with the dropping liquid gear of dropping liquid rack 1d4 meshing, dropping liquid gear passes through the internal spline and installs on dropping liquid spline axle 1d6 along Y to sliding suit, dropping liquid spline shaft 1d6 is arranged and can install on the rotation along Y to little crossbeam 1c, dropping liquid spline 1d6 is driven by dropping liquid motor 1d7, correspond on little crossbeam 1c the dropping liquid slider 36 is equipped with Y to Y position moves to the dropping liquid plunger pump 1d5 d8, the dropping liquid Y to removal belt 1d8 with dropping liquid slider 1d5 links to each other, be equipped with on the little crossbeam 1c and be used for the drive dropping liquid Y is to the dropping liquid belt feeder 1d9 of removal belt 1d8 action. The liquid suction and titration of the dropping burette 1d1 are controlled by the dropping plunger pump 1d3, so that the dropping amount of the liquid can be accurately controlled; simultaneously, through the X of the little crossbeam 1c of control to removing, dropping liquid subassembly 1dY to with Z to the removal, can realize the accurate control of dropping liquid buret 1d1 position, the control titration amount of deuterogamying to the liquid drop position, the size shape rule of control titration that can be accurate. And a first liquid level sensor 1d10 for detecting the liquid level height of the liquid is arranged on the small beam 1 c. The added first liquid level sensor 1d10 can accurately detect the liquid level height of the liquid, thus conveniently and accurately controlling the depth of the dropping burette 1d1 going deep into the liquid bottle to ensure smooth liquid suction.
The number of the small beams 1c is two, the dropping liquid assembly 1d is mounted on one of the small beams 1c, the other of the small beams 1c is movably mounted with an oil covering assembly 1e along the Y direction, the oil covering assembly 1e comprises an oil covering fixed pipe 1e1, an oil covering shaft 1e2 and an oil covering plunger pump 1e3, the upper end of the oil covering fixed pipe 1e1 is connected with the lower end of the oil covering shaft 1e2, the upper end of the oil covering shaft 1e2 is communicated with the oil covering plunger pump 1e3, the oil covering plunger pump 1e3 is mounted on the upper end of the oil covering shaft 1e2 or the corresponding small beam 1c, the oil covering shaft 1e2 is arranged along the Z direction and is provided with an oil covering rack 1e4 along the axial direction, the oil covering shaft 1e2 is provided with an oil covering slider 1e5 in a position corresponding to the oil covering rack 1e4 along the axial direction, the oil covering slider 5 is provided with an oil covering rack 4 engaged with the oil covering rack 4, the oil covering gear is sleeved on an oil covering spline shaft 1e6 in a sliding mode along the Y direction through an internal spline, the oil covering spline shaft 1e6 is arranged along the Y direction and is installed on the corresponding small cross beam 1c in a rotatable mode, the oil covering spline shaft 1e6 is driven to rotate by an oil covering motor 1e7, an oil covering Y-direction moving belt 1e8 is arranged on the corresponding small cross beam 1c in a position corresponding to the oil covering slider 1e5, the oil covering Y-direction moving belt 1e8 is connected with the oil covering slider 1e5, and an oil covering belt machine 1e9 used for driving the oil covering Y-direction moving belt 1e8 to move is arranged on the corresponding small cross beam 1 c. The oil sucking and titration of the oil-covered burette 1e1 are controlled by the oil-covered plunger pump 1e3, so that the titration amount of the oil can be accurately controlled; simultaneously, through the X of control little crossbeam 1c to removing, cover oily subassembly 1eY to with Z to the removal, can realize covering the accurate control of oil buret 1e1 position, the control titration amount of deuterogamy to the position and the oil mass of oily lid of control ability are accurate. And a second liquid level sensor 1e10 for detecting the height of the oil liquid level is arranged on the corresponding small cross beam 1 c. The second liquid level sensor 1e10 that sets up can accurately detect out the liquid level height of fluid, and convenient accurate control lid oil buret 1e1 is deepened the degree in the oil bottle like this to guarantee that the oil absorption is smooth and easy.
Install dropping liquid subassembly 1d it has dish lid operation subassembly 1f to remove to install along the Y on little crossbeam 1c, dish lid operation subassembly 1f is including inhaling lid pipe 1f1 and inhaling lid axle 1f2, inhale lid pipe 1f1 upper end with it links to each other to inhale lid axle 1f2 lower extreme, inhale lid axle 1f2 upper end intercommunication negative pressure source, inhale lid axle 1f2 along Z to arrange and be equipped with along the axial on it and inhale lid rack 1f4, it corresponds on the lid axle 1f2 inhale the position of lid rack 1f4 and install along axial slidable and inhale lid slider 1f5, be equipped with on the lid slider 1f5 with inhale the lid gear that lid rack 1f4 meshed, inhale the lid gear through the internal spline along Y to the slip suit on inhaling lid integral key shaft 1f6, inhale lid 1f6 along Y to arrange and can install corresponding on little crossbeam 1c with the rotation, the sucking cover spline shaft 1f6 is driven by a sucking cover motor 1f7 to rotate, and the dropping liquid Y is connected with the sucking cover slider 1f5 to the moving belt 1d 8. The cover suction pipe 1f1 is matched with a negative pressure source to control the taking and the placing of the dish box cover, so that the whole process of the preparation of the dish box is automated; meanwhile, the capsule cover can be flexibly taken and placed by controlling the X-direction movement of the small beam 1c and the movement of the capsule cover operation assembly 1fY and Z-direction. Inhale and cover slider 1f5 and go up to correspond the position of dropping liquid spline shaft 1d6 be equipped with dropping liquid spline shaft 1d6 matched with inhale and cover the slider guiding hole, it corresponds on dropping liquid slider 1d5 inhale the position of covering spline shaft 1f6 be equipped with inhale and cover spline shaft 1f6 matched with dropping liquid slider guiding hole. Add the suction cap slider guiding hole and the dropping liquid slider guiding hole of establishing and can avoid taking place the action to interfere between dropping liquid subassembly 1d and the ware box cover operation module 1f on the one hand, on the other hand can also play the guide effect to the stationarity of action has been improved.
Of course, only one small beam 1c can be designed, and the dropping liquid assembly 1d, the oil covering assembly 1e and the dish box cover operating assembly 1f are arranged on the same small beam 1 c; two small beams 1c can also be designed, and the drip component 1d, the oil covering component 1e and the dish box cover operating component 1f are combined on one small beam 1c in a pairwise different manner; three small beams 1c can be designed, and the dropping liquid assembly 1d, the oil covering assembly 1e and the dish box cover operating assembly 1f are respectively arranged on the different small beams 1 c.
This embodiment still includes dish box removal subassembly 1g, dish box removal subassembly 1g includes that dish box slide rail 1g1, dish box cooling plate 1g2 and dish box place platform 1g3, dish box cooling plate 1g2 slidable mounting is in on the dish box slide rail 1g1, the dish box is placed platform 1g3 and is installed on the dish box cooling plate 1g 2. By designing the dish box moving assembly 1g into a movable structure, the whole process automation of the liquid preparation action and the whole process automation of putting the finished products into the incubator 3 can be simply realized by matching with the transfer mechanism 2; meanwhile, the additional dish box cooling plate 1g2 can prevent the liquid from volatilizing. The dish box placing table 1g3 is provided with a tray positioning and placing groove on the top surface, and the two sides of the tray positioning and placing groove are provided with clamping jaw avoiding grooves convenient for clamping. The dish box moving assembly 1g is positioned between the two small beams 1c, and the moving direction of the dish box moving assembly 1g is perpendicular to the moving direction of the small beams 1 c.
The dish box moving assembly 1g side is further provided with an oil liquid bottle placing assembly 1h, the oil liquid bottle placing assembly 1h comprises an oil liquid bottle placing positioning frame 1h1, and the oil liquid bottle placing positioning frame 1h1 bottom surface is provided with an oil liquid bottle cooling plate 1h 2. The positioning frame 1h1 is provided with steps and grooves with different sizes, and can be suitable for oil bottles or containers with different sizes and heights. By designing the oil bottle cooling plate 1h2 in the oil bottle placing assembly 1h, the volatilization of liquid and oil can be reduced. The quantity of subassembly 1h is placed to the fluid bottle is two sets of, and a set of is liquid, and a set of fluid, and two sets of fluid bottles are placed subassembly 1h and are arranged respectively in the both sides that the ware box removed subassembly 1 g.
The transfer mechanism 2 includes a traverse robot 2a, a lift robot 2b, and a gripper 2c, the gripper 2c is mounted on a lift portion of the lift robot 2b, and a mounting portion of the lift robot 2b is mounted on a traverse portion of the traverse robot 2 a. The moving direction of the traverse robot arm 2a is arranged in parallel with the moving direction of the small beam 1 c.
Above-mentioned incubator 3 includes box 3a, box 3a internal fixation is equipped with a plurality of and cultivates bin 3b, box 3a corresponds the position of cultivateing a bin 3b outside is equipped with drawer rail 3c, box 3a corresponds the position of cultivateing another outside of bin 3b is equipped with cang gai drive assembly 3d, drawer rail 3 c's outer end with cang gai drive assembly 3 d's outer end corresponds the position of cultivateing bin 3b is equipped with cang gai 3e, the internal surface of cang gai 3e is equipped with bracket 3 g. By designing the drawer rail 3c at the periphery of the incubation chamber 3b, it is possible to prevent the oil in the drawer rail 3c from contaminating the incubation chamber 3 b.
Above-mentioned storehouse lid drive assembly 3d is including installing respectively storehouse lid belt feeder 3d1 and storehouse lid drive belt 3d2 in the box 3a, storehouse lid drive belt 3d2 by storehouse lid belt feeder 3d1 drive is equipped with drawer pole 3d3 on the storehouse lid drive belt 3d2, the outer end of drawer pole 3d3 with storehouse lid 3e links to each other. And an anti-condensation heating film is arranged on the bin cover 3 e. The added anti-condensation heating film can avoid condensation on the surface of the bin cover 3 e. An air seal ring 3i which is matched with the mouth of the culture chamber 3b is further arranged on the inner surface of the chamber cover 3 e. The addition of the gas seal ring 3i can improve the sealing performance of the culture chamber 3 b.
A bin cover position sensor assembly 3f for monitoring the position of the bin cover 3e is arranged in the box body 3 a. Storehouse lid position sensor subassembly 3f includes cang gai position contact 3f1 and storehouse lid inductor 3f2 of mutually supporting, cang gai position contact 3f1 is installed cang gai drive belt 3d2 with on the connecting portion of drawer pole 3d3, two cang gai inductor 3f2 is installed respectively correspond in the box 3a the position at storehouse lid drive belt 3d2 both ends.
This embodiment is still including the tray 4 that is used for placing the dish box, it accomplishes to be equipped with liquid mechanism 1 after the dish box on tray 4 is equipped with liquid and moves, it will to move and carry out the preliminary treatment in tray 4 puts into incubator 3, be equipped with the tray location structure that is used for the location to place the tray on the bottom plate, like the reference column etc.. The tray 4 comprises a tray body 4a, and a first dish box placing positioning hole 4b is formed in the top surface of the tray body 4 a. The first dish box placing positioning hole 4b is additionally arranged, so that the dish box can be placed and positioned conveniently, and meanwhile, conditions are provided for automatic liquid preparation. The top surface of the tray body 4a is also provided with a stacking and positioning support column 4 c. The stacking positioning support columns 4c can stack the trays in multiple layers, so that the occupied area of the trays is saved, and the liquid preparation of the trays can be realized. And a stacking positioning hole 4d matched with the stacking positioning support column 4c is formed in the position, corresponding to the stacking positioning support column 4c, of the bottom surface of the tray body 4 a. And clamping jaw grabbing grooves 4e are respectively formed in two sides of the bottom surface of the tray body 4 a. The clamping jaw grabbing groove 4e is additionally arranged, so that the clamping jaw can grab the tray smoothly. The bottom surface of the tray body 4a is also provided with a two-dimensional code groove 4 f. The two-dimensional code can be marked in the two-dimensional code groove 4f, so that the information of each tray can be marked. The tray body 4a is provided with a positioning guide hole 4 g. The positioning guide hole 4g is matched with the tray positioning structure. The additional positioning guide holes 4g facilitate the positioning of the tray. The tray body 4a is also provided with lightening holes 4 h. The additional lightening holes 4h can effectively lighten the weight of the tray without influencing the strength and the use function of the tray. The tray body 4a is a 96-hole plate structure. The number of the first dish box placing positioning holes 4b is 6, and the 6 first dish box placing positioning holes 4b are uniformly arranged. The position, corresponding to the first dish box placing positioning hole 4b, of the top surface of the tray body 4a is concentrically provided with second dish box placing positioning holes 4i with different diameters, and the second dish box placing positioning holes 4i and the first dish box placing positioning holes 4b are distributed on different horizontal surfaces. The positioning holes are arranged through the dish boxes with different diameters, so that the tray can be suitable for dish boxes of different models, and the applicability of the tray is improved. The number of the positioning guide holes 4g is 6, and the 6 positioning guide holes 4g are symmetrically arranged. Through the quantity design of locating guiding hole 4g for 6, and 6 locating guiding hole 4g symmetrical arrangement, when the tray was placed in the location like this, can not divide the direction of tray.
The liquid preparation process of this example is as follows:
firstly, putting a certain number of empty dish boxes a into a corresponding number of trays 4, and stacking the trays 4 filled with the dish boxes a at the position of a tray positioning structure on a bottom plate; then, correspondingly and respectively placing the oil/liquid bottles at the oil bottle placing component for 1 h; and starting the liquid preparation. At the moment, the liquid preparation instrument firstly controls the transfer mechanism 2 to place the tray 4 at the topmost layer of the tray positioning structure on the dish box placing table 1g3 through the clamping jaw 2c, then controls the dish box moving assembly 1g to move the tray to the liquid preparation station, then controls the dish box cover operating assembly 1f to suck the cover of the dish box and move away, then controls the dropping liquid assembly 1d to titrate the liquid, controls the oil covering assembly 1e to cover the oil after the liquid is titrated, and controls the dish box cover operating assembly 1f to cover the cover of the dish box, and the process is circulated until all the dish boxes a on the same tray 4 are completely prepared with the liquid; the liquid preparation instrument controls the dish box moving assembly 1g to move the tray 4 with the liquid preparation completed back to the initial position, and the transfer mechanism 2 moves the tray 4 with the liquid preparation completed to the incubator 3 through the clamping jaws 2 c; then, the liquid preparation instrument controls the opening of a bin cover 3e of the incubator 3, controls the clamping jaws 2c to place the tray 4 after the liquid preparation is finished on a bracket 3g on the bin cover 3e, and finally controls the bin cover 3e to be closed, so that the liquid preparation of one tray 4 is finished; the liquid preparation instrument controls the clamping jaw 2c to pick the second tray 4 on the tray positioning structure, and the process is circulated until the liquid preparation of all the trays 4 is completed.
And a two-dimensional code scanner 1j is arranged on the bottom plate corresponding to the position between the tray positioning structure and the dish box placing table 1g 3. Like this, place the in-process on platform 1g3 is placed to the dish box at the holding jaw 2c with tray 4 of tray location structure topmost, and the information of dish box on the tray can be acquireed fast to the two-dimensional code in this two-dimensional code scanner 1j cooperation tray body 4a bottom surface two-dimensional code groove 4 f. A tray presence sensor 3h for detecting the presence of a vacant position on the bracket 3g when the bin cover 3e is ejected is arranged on the bottom plate at a position corresponding to the ejection of the bin cover 3 e. The tray presence/absence sensor 3h is provided to ensure that the tray is placed on the tray 3g only when the tray presence/absence sensor 3h detects that the tray 4 is not present on the tray 3 g.
In the embodiment, the liquid preparation mechanism 1 automatically completes the liquid preparation action, so that the position, the size and the shape of a titrated liquid drop are regular, the titrating amount of the liquid can be accurately controlled, and the oil covering amount of the liquid can also be accurately controlled; after the liquid preparation action is finished, the titrated liquid can be immediately put into a culture box 3 for pretreatment by a transfer mechanism 2; finally, the whole liquid preparation process does not need manual intervention, thereby greatly reducing the labor cost.
Meanwhile, in the present embodiment, the liquid suction and the dropping of the dropping burette 1d1 are controlled by the dropping plunger pump 1d3, so that the dropping amount of the liquid can be accurately controlled; by controlling the X-direction movement of the small beam 1c and the dY-direction and Z-direction movement of the dropping component 1d, the accurate control of the position of the dropping burette 1d1 can be realized, and the dropping quantity is controlled in a matched manner, so that the position, size and shape rules of the dropping liquid for titration can be accurately controlled; the oil absorption and titration of the oil-covered burette 1e1 are controlled by the oil-covered plunger pump 1e3, so that the titration amount of the oil liquid can be accurately controlled; by controlling the X-direction movement of the small beam 1c and the movement of the oil covering component 1eY in the Z direction, the accurate control of the position of the oil covering burette 1e1 can be realized, and the dropping amount is controlled in a matched manner, so that the position and the oil amount of the oil covering can be accurately controlled; the cover suction pipe 1f1 is matched with a negative pressure source to control the taking and placing of the dish box cover, so that the whole process of preparing liquid in the dish box can be automated; the capsule cover can be flexibly taken and placed by controlling the X-direction movement of the small beam 1c and the movement of the capsule cover operation assembly 1fY direction and Z direction; the additionally arranged guide hole of the suction cover sliding block and the guide hole of the dropping liquid sliding block can prevent the dropping liquid assembly 1d from acting and interfering with the dish box cover operating assembly 1f on one hand, and can also play a role in guiding on the other hand, thereby improving the stability of action; in addition, the added liquid level sensor can accurately detect the liquid level height of the oil liquid, so that the depth of the burette penetrating into the oil liquid bottle can be conveniently and accurately controlled, and the smoothness of the oil absorption liquid is ensured; .
In addition, in the embodiment, the dish box moving assembly 1g is designed to be a movable structure, so that the whole process automation of the liquid preparation action and the whole process automation of putting the finished products into the incubator 3 can be simply realized by matching with the transfer mechanism 2; meanwhile, the additionally arranged dish box cooling plate 1g2 can prevent the liquid from volatilizing; by designing the oil liquid bottle cooling plate 1h2 in the oil liquid bottle placing component 1h, the volatilization of liquid and oil liquid can be reduced.
Furthermore, in the embodiment, the drawer rail 3c is designed at the periphery of the culture chamber 3b, so that the oil in the drawer rail 3c can be prevented from polluting the culture chamber 3 b; the added anti-condensation heating film can avoid condensation on the surface of the bin cover 3 e; the additionally arranged tray presence or absence sensor 3h can ensure that the tray is placed on the bracket 3g after the tray presence or absence sensor 3h detects that the tray 4 is not on the bracket 3 g; the added gas sealing ring 3i can improve the sealing performance of the culture chamber 3 b.
Finally, the additionally arranged first dish box placing positioning hole 4b facilitates placing and positioning of the dish box and provides conditions for automatic liquid preparation; the additional stacking positioning support columns 4c can realize the multilayer stacking of the trays, so that the floor area of the trays is saved, and the liquid preparation of a plurality of trays can be realized; the additional clamping jaw grabbing grooves 4e facilitate the clamping jaws to grab the tray smoothly; the two-dimensional code can be marked in the additionally arranged two-dimensional code groove 4f, so that the information of each tray can be marked; the additional positioning guide holes 4g facilitate the positioning of the tray; the additional lightening holes 4h can effectively lighten the weight of the tray without influencing the strength and the use function of the tray; through designing the dish box placing positioning holes with different diameters, the tray can be suitable for dish boxes with different models, so that the applicability of the tray is improved; the number of the positioning guide holes 4g is designed to be 6, and the 6 positioning guide holes 4g are symmetrically arranged, so that the direction of the tray can not be divided when the tray is positioned and placed; the two-dimensional code scanner 1j cooperates the two-dimensional code in the two-dimensional code groove 4f on the bottom surface of the tray body 4a to rapidly acquire the information of the tray upper dish box.

Claims (10)

1. A culture dish tray, its characterized in that: the tray comprises a tray body (4a), wherein a first dish box placing positioning hole (4b) is formed in the top surface of the tray body (4 a).
2. A petri dish tray, as claimed in claim 1, wherein: the top surface of the tray body (4a) is also provided with stacking and positioning support columns (4 c).
3. A petri dish tray as claimed in claim 2, wherein: the bottom surface of the tray body (4a) is provided with a stacking positioning hole (4d) corresponding to the stacking positioning support column (4c) and matched with the stacking positioning support column (4 c).
4. A petri dish tray, as claimed in claim 1, wherein: clamping jaw grabbing grooves (4e) are respectively formed in two sides of the bottom surface of the tray body (4 a).
5. A petri dish tray, as claimed in claim 1, wherein: the bottom surface of the tray body (4a) is also provided with a two-dimensional code groove (4 f).
6. A petri dish tray, as claimed in claim 1, wherein: be equipped with location guiding hole (4g) on tray body (4a), the quantity of location guiding hole (4g) is 6, and 6 location guiding hole (4g) symmetrical arrangement.
7. A petri dish tray, as claimed in claim 1, wherein: the tray body (4a) is also provided with lightening holes (4 h).
8. A petri dish tray, as claimed in claim 1, wherein: the tray body (4a) is of a 96-hole plate structure.
9. A petri dish tray, as claimed in claim 1, wherein: the number of the first dish box placing positioning holes (4b) is 6, and the 6 first dish box placing positioning holes (4b) are uniformly distributed.
10. A petri dish tray, as claimed in claim 1, wherein: the top surface of the tray body (4a) is concentrically provided with second dish box placing positioning holes (4i) with different diameters corresponding to the first dish box placing positioning holes (4b), and the second dish box placing positioning holes (4i) and the first dish box placing positioning holes (4b) are distributed on different horizontal planes.
CN202011007906.8A 2020-09-23 2020-09-23 Culture dish tray Pending CN112159750A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202011007906.8A CN112159750A (en) 2020-09-23 2020-09-23 Culture dish tray

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202011007906.8A CN112159750A (en) 2020-09-23 2020-09-23 Culture dish tray

Publications (1)

Publication Number Publication Date
CN112159750A true CN112159750A (en) 2021-01-01

Family

ID=73863372

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202011007906.8A Pending CN112159750A (en) 2020-09-23 2020-09-23 Culture dish tray

Country Status (1)

Country Link
CN (1) CN112159750A (en)

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