CN112147080A - Free space type Mueller OCT imaging system and imaging method for full-automatic time-division detection - Google Patents
Free space type Mueller OCT imaging system and imaging method for full-automatic time-division detection Download PDFInfo
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Abstract
The invention discloses a free space type Mueller OCT imaging system and an imaging method for full-automatic time-division detection, wherein four optical channels with different polarization types are arranged in a reference arm: horizontal, vertical, 45 ° linear polarization and right-hand circular polarization. Meanwhile, the sample arm uses a software program to control the precision rotating workbench to rotate the wave plate so as to realize the polarized light of the four states. The reference wall also uses software program to control the optical switch to select different channels in the reference arm, and the polarized light of each channel returns to interfere with the polarized light scattered back by the sample arm, thereby realizing the time division detection of the polarized information. And meanwhile, 16 element images of the Mueller matrix of the sample are reconstructed, so that the acquisition of complete polarization information is realized. The invention realizes full-automatic control, can quickly acquire images and improves the sampling rate.
Description
Technical Field
The invention relates to the field of biomedicine and optics, in particular to a full-automatic time-division detection free-space Mueller OCT imaging system and an imaging method.
Background
At present, the biological imaging technology mainly comprises the following steps: ultrasound imaging, X-ray imaging, magnetic resonance diffusion imaging techniques and optical coherence tomography techniques. Wherein, the methods of ultrasonic imaging, X-ray imaging and the like are easy to cause deformation to tissues and damage to tissue structures, the imaging resolution ratio of the tissue structure is not high, the micro structure in the tissue can not be observed, and the novel optical coherence tomography technology has the advantages of no damage, high resolution ratio and the like, can perfectly image the tissue microstructure, in particular to a new polarized optical coherence tomography technology developed by a Mueller OCT imaging system on the basis of the traditional OCT, which can provide the sample structure information as the traditional OCT and can also obtain the polarization information of the sample, when the linearly polarized light is incident to the sample to be measured, because the sample changes the polarization property of the scattered back light, the polarization information of the sample can be obtained by detecting the polarization characteristic of the scattered back light, and the Mueller OCT imaging system can completely acquire the polarization information of the sample.
At present, a commonly used polarization OCT uses a jones matrix to deduce a Mueller matrix for describing structural features of biological tissues, but this method cannot completely describe polarization characteristics of biological samples, so that it is necessary to build a Mueller OCT system, and a reference wall of a Mueller OCT system built by an existing team uses an adjustable rotating wave plate to manually adjust a rotation angle of the rotating wave plate to change a polarization state, but this manual adjustment mode can reduce an acquisition rate, greatly reduce experimental efficiency, and generate an error by manually adjusting scales, so that a certain fluctuation is generated by the change of the polarization state, and the acquired data has a deviation, so that it is necessary to provide a free space type Mueller OCT imaging system with full-automatic time division detection, four paths of the reference wall of the system are independent and horizontal respectively, the device comprises a vertical 45-degree linearly polarized light source, a right-handed circularly polarized light source, a sample wall, a precision rotating worktable, a light switch, a light source, a.
Therefore, the defect of the above problems is that a fully automatic time-division detection free-space Mueller OCT imaging system is required to be provided.
Disclosure of Invention
The invention aims to provide a free space type Mueller OCT imaging system and an imaging method for full-automatic time-division detection.
The technical scheme adopted by the invention is as follows:
the free space type Mueller OCT imaging system with full-automatic time-division detection comprises a low-coherence broadband polarized light source and a first non-polarized beam splitter, wherein the low-coherence broadband polarized light source enters the first non-polarized beam splitter to be divided into reflected light serving as reference light and transmitted light serving as sample light,
the reference light enters a second non-polarization beam splitter, a third non-polarization beam splitter is arranged on a transmission light path of the second non-polarization beam splitter, a fourth non-polarization beam splitter is arranged on a reflection light path of the second non-polarization beam splitter, the reference light forms 4 paths of reference emergent light through the third non-polarization beam splitter and the fourth non-polarization beam splitter, different polarization light path structures with optical switches are respectively arranged on each path of reference emergent light, and the reference light respectively obtains horizontal polarization, vertical polarization, 45-degree linear polarization and right-hand circular polarization and is connected into the first non-polarization beam splitter along a light path through reflection; the sample light is projected to a sample to be measured sequentially through the third quarter-wave plate, the half-wave plate, the three-dimensional scanning galvanometer and the focusing objective lens; the first non-polarization beam splitter is provided with a spectrometer corresponding to the opposite surface of the emergent surface of the reflected light, the spectrometer receives the reflected polarized light, the spectrometer is connected with computer data acquisition equipment, and the computer data acquisition equipment is respectively connected with and controls an optical switch of each path of polarized light path structure to select the reference light corresponding to polarization; and the computer data acquisition equipment is connected with and controls the precise rotating workbench where the third quarter-wave plate and the half-wave plate are located, and the computer data acquisition equipment controls the precise rotating workbench of the third quarter-wave plate and the half-wave plate to rotate based on the selected polarized reference light so as to obtain the sample polarized light which is the same as the reference wall.
Further, as a preferred embodiment: the low-coherence broadband polarized light source enters the first non-polarized beam splitter through the first collimating lens, and the receiving end of the spectrometer is provided with the second collimating lens.
Further, as a preferred embodiment, the low coherence broadband polarized light source is connected to a polarization maintaining drill rod and connected to the first collimating mirror through the polarization maintaining drill rod, and the receiving end of the spectrometer is connected to the second collimating mirror through the polarization maintaining drill rod.
Further, as a preferred embodiment, the four optical path structures with different polarizations are respectively a horizontal polarization optical path structure, a vertical polarization optical path structure, a 45-degree linear polarization optical path structure and a right-handed circular polarization optical path structure;
the horizontally polarized light path structure is arranged on a transmission light path of the third non-polarized beam splitter and comprises a first diaphragm, a first optical switch and a first reflector which are sequentially arranged along the light path;
the vertical polarization light path structure is arranged on a reflection light path of the third non-polarization beam splitter and comprises a second diaphragm, a second optical switch, a first quarter wave plate and a second reflector which are sequentially arranged along the light path;
the 45-degree linear polarization light path structure is arranged on a reflection light path of the fourth non-polarization beam splitter, and the vertical polarization light path structure comprises a third diaphragm, a third light switch, a second quarter wave plate and a third reflector which are sequentially arranged along the light path;
the right-handed circular polarization light path structure is arranged on a transmission light path of the fourth non-polarization beam splitter, and the vertical polarization light path structure comprises a fourth diaphragm, a fourth optical switch, an eighth wave plate and a fourth reflector which are sequentially arranged along the light path.
Further, as a preferred embodiment, the low coherence broadband polarized light source adopts a super-radiation light emitting diode or a swept-frequency broadband light source.
Further, as a preferred embodiment, the super luminescent diode or swept broadband light source wavelength is 1310 nm.
Further, as a preferred embodiment, the low coherence broadband polarized light source has a bandwidth in the range of tens of nanometers to hundreds of nanometers.
Further, as a preferred embodiment, the splitting ratio of the first non-polarizing beam splitter, the second non-polarizing beam splitter, the third non-polarizing beam splitter and the fourth non-polarizing beam splitter is 50: 50 applicable wavelength range is 1100nm ~1600nm beam splitter.
Further, as a preferred embodiment, the spectrometer is a michelson interferometer or a mach-zehnder interferometer.
Furthermore, the invention also discloses an imaging method of the full-automatic time-division detection free-space Mueller OCT imaging system, which is characterized in that: the imaging method comprises the following steps:
(1) light emitted by the low-coherence broadband polarized light source is connected with the first collimating mirror through the polarization-maintaining optical fiber and is divided into reference light and sample light through the first non-polarized beam splitter.
(2) The reference light is divided into four paths of reference light sequentially through the second non-polarization beam splitter, the third non-polarization beam splitter and the fourth non-polarization beam splitter, and the four paths of reference light returned by the reflector are respectively four different types of polarized light: horizontally, vertically, 45-degree linearly polarized light and rightly-polarized light;
(3) the optical path of the four paths of reference light is equal to that of the sample wall through adjustment, the sample light is projected to a sample to be measured through the quarter-wave plate and the half-wave plate, the quarter-wave plate and the half-wave plate are quickly rotated by utilizing a precise rotating workbench, so that four polarized lights identical to the reference wall are adjusted, and the polarization information carried by the sample light after being reflected inside the sample is coupled into a spectrometer to interfere with the four paths of light on the reference wall;
(4) the four paths of reference light and the sample light are interfered and then respectively provided with polarization information of horizontal, vertical, 45-degree and right-handed circles, the reference wall utilizes the optical switch to perform time-sharing detection on four paths of optical channels and respectively interfere with the four paths of light on the sample wall, interference spectrum signals recorded by the spectrograph are collected by the video acquisition card and then are transmitted to the computer for data processing, and 16-element information of a Mueller matrix of the sample, namely a complete polarization characteristic image of the sample, is obtained through data reconstruction.
The invention can completely present the polarization information of the sample, can rapidly acquire the 16 OCT image data of different polarization states by automatic control, is used for calculating the Mueller matrix, and simultaneously improves the acquisition rate of the sample, and the Mueller OCT imaging system is a polarization optical coherence tomography technology with no damage and high resolution.
Drawings
The invention is described in further detail below with reference to the accompanying drawings and the detailed description;
FIG. 1 is a schematic structural diagram of a fully automatic time-division detection free-space Mueller OCT imaging system of the present invention;
description of reference numerals:
1-low coherence broadband polarized light source; 2-a first collimating mirror; 3-a first non-polarizing beam splitter; 4-a second non-polarizing beam splitter; 5-a third non-polarizing beam splitter; 6-a first diaphragm; 7-a first optical switch; 8-a first mirror; 9-a second diaphragm; 10-a second optical switch; 11-a first quarter wave plate; 12-a second mirror; 13-a fourth non-polarizing beam splitter; 14-a third diaphragm; 15-a third optical switch; 16-a second quarter wave plate; 17-a third mirror; 18-fourth diaphragm; 19-a fourth optical switch; a 20-eighth wave plate; 21-a fourth mirror; 22-a third quarter wave plate; 23-a half wave plate; 24-three-dimensional scanning galvanometer; 25-a focusing objective lens; 26-a sample to be tested; 27-a second collimating mirror; 28-a spectrometer; 29-computer data acquisition equipment.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions of the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application.
As shown in FIG. 1, the invention discloses a fully automatic time-division detection free-space type Mueller OCT imaging system, which comprises a low-coherence broadband polarized light source 1, a first collimating mirror 2, a first non-polarized beam splitter 3, a second non-polarized beam splitter 4, a third non-polarized beam splitter 5, a first diaphragm 6, a first optical switch 7, a first reflector 8, a second diaphragm 9, a second optical switch 10, a first quarter wave plate 11, a second reflector 12, a fourth non-polarized beam splitter 13, a third diaphragm 14, a third optical switch 15, a second quarter wave plate 16, a third reflector 17, a fourth diaphragm 18, a fourth optical switch 19, an eighth wave plate 20, a fourth reflector 21, a third quarter wave plate 22, a half wave plate 23, a three-dimensional scanning galvanometer 24, a focusing objective lens 25, a sample to be measured 26, a second collimating mirror 27, a spectrometer 28, and a computer data acquisition device 29, the low-coherence broadband polarized light source is connected with a polarization maintaining optical fiber, the polarization maintaining optical fiber is respectively connected with a first collimating mirror, a second collimating mirror and a spectrometer, the first collimating mirror is connected with a first non-polarized beam splitter, the first non-polarized beam splitter is respectively connected with a second non-polarized beam splitter, the second non-polarized beam splitter is respectively connected with a third non-polarized beam splitter and a fourth non-polarized beam splitter, the third non-polarized beam splitter is respectively connected with a first diaphragm, a first optical switch, a first reflecting mirror, a second diaphragm, a second optical switch, a first quarter wave plate and a second reflecting mirror, the fourth non-polarized beam splitter is respectively connected with a third diaphragm, a third optical switch, a second quarter wave plate, a third reflecting mirror, a fourth diaphragm, a fourth optical switch, an eighth wave plate and a fourth reflecting mirror, and the second collimating mirror sequentially passes through the third quarter wave plate, the second quarter wave plate, the fourth diaphragm, the fourth optical switch, the eighth wave plate and the fourth reflecting mirror, The half wave plate, the three-dimensional scanning galvanometer and the focusing objective lens are connected with a sample to be measured, and the spectrometer is connected with computer data acquisition equipment.
Further, the low coherence broadband polarized light source adopts a super-radiation light emitting diode or a swept-frequency broadband light source.
Further, the wavelength of the super-radiation light emitting diode or the swept broadband light source is 1310 nm.
Further, the bandwidth of the low coherence broadband polarized light source ranges from tens of nanometers to hundreds of nanometers.
Further, the splitting ratio of the first non-polarizing beam splitter, the second non-polarizing beam splitter, the third non-polarizing beam splitter and the fourth non-polarizing beam splitter is 50: 50 applicable wavelength range is 1100nm ~1600nm beam splitter.
Further, the spectrometer is a michelson interferometer or a mach-zehnder interferometer.
Full-automatic time-division detection's fiber type Mueller OCT imaging system, its characterized in that: the working process comprises the following steps: (1) light emitted by the low-coherence broadband polarized light source is connected with the first collimating mirror through the polarization maintaining optical fiber and is divided into reference light and sample light through the first non-polarized beam splitter. (2) The reference light is divided into four paths of reference light sequentially through the second non-polarization beam splitter, the third non-polarization beam splitter and the fourth non-polarization beam splitter, and the four paths of reference light returned by the reflector are respectively four different types of polarized light: horizontal, vertical, 45 ° linearly polarized light and dextrorotatory circularly polarized light. (3) The light path of the four paths of reference light is equal to that of the sample wall through adjustment, the sample light passes through the quarter-wave plate and the half-wave plate, four polarized lights identical to the reference wall are rapidly adjusted through rotation of the precision rotating worktable, and polarization information carried by the sample light after being reflected inside the sample is coupled into the Michelson interferometer to interfere with the four paths of light of the reference wall. (4) Four paths of reference light and sample light are interfered and then respectively provided with polarization information of horizontal, vertical, 45-degree and right-handed circles, a reference wall utilizes an optical switch to perform time-sharing detection on four paths of optical channels and respectively interfere with the four paths of light on the sample wall, interference spectrum signals recorded by a spectrometer are collected by a video acquisition card and then are transmitted to a computer for data processing, and data reconstruction is carried out to obtain 16-element information of a Mueller matrix of the sample, namely complete polarization characteristic image of the sample
The invention can completely present the polarization information of the sample, can rapidly acquire the 16 OCT image data of different polarization states by automatic control, is used for calculating the Mueller matrix and improves the acquisition rate, and the Mueller OCT imaging system is a nondestructive and high-resolution polarized optical coherence tomography technology.
It is to be understood that the embodiments described are only a few embodiments of the present application and not all embodiments. The embodiments and features of the embodiments in the present application may be combined with each other without conflict. The components of the embodiments of the present application, generally described and illustrated in the figures herein, can be arranged and designed in a wide variety of different configurations. Thus, the detailed description of the embodiments of the present application is not intended to limit the scope of the claimed application, but is merely representative of selected embodiments of the application. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
Claims (10)
1. Full-automatic time-division detection's free space type Mueller OCT imaging system, its characterized in that: which comprises a low-coherence broadband polarized light source and a first non-polarized beam splitter, wherein the low-coherence broadband polarized light source enters the first non-polarized beam splitter to be split into reflected light serving as reference light and transmitted light serving as sample light,
the reference light enters a second non-polarization beam splitter, a third non-polarization beam splitter is arranged on a transmission light path of the second non-polarization beam splitter, a fourth non-polarization beam splitter is arranged on a reflection light path of the second non-polarization beam splitter, the reference light forms 4 paths of reference emergent light through the third non-polarization beam splitter and the fourth non-polarization beam splitter, different polarization light path structures with optical switches are respectively arranged on each path of reference emergent light, and the reference light which is horizontally polarized, vertically polarized, linearly polarized at 45 degrees and circularly polarized in a right-handed mode is respectively obtained and is connected to the first non-polarization beam splitter along a light path through reflection; the sample light is projected to a sample to be measured sequentially through the third quarter-wave plate, the half-wave plate, the three-dimensional scanning galvanometer and the focusing objective lens; the first non-polarization beam splitter is provided with a spectrometer corresponding to the opposite surface of the emergent surface of the reflected light, the spectrometer receives the reflected polarized light, the spectrometer is connected with computer data acquisition equipment, and the computer data acquisition equipment is respectively connected with and controls an optical switch of each path of polarized light path structure to select the reference light corresponding to polarization; and the computer data acquisition equipment is connected with and controls the precise rotating workbench where the third quarter-wave plate and the half-wave plate are located, and the computer data acquisition equipment controls the precise rotating workbench of the third quarter-wave plate and the half-wave plate to rotate based on the selected polarized reference light so as to obtain the sample polarized light which is the same as the reference wall.
2. The fully automated time-resolved detection free-space-type Mueller OCT imaging system of claim 1, wherein: the low-coherence broadband polarized light source enters the first non-polarized beam splitter through the first collimating lens, and the receiving end of the spectrometer is provided with the second collimating lens.
3. The fully automated time-resolved detection free-space-type Mueller OCT imaging system of claim 2, wherein: the low-coherence broadband polarized light source is connected with a polarization maintaining drill rod and is connected into the first collimating lens through the polarization maintaining drill rod, and the receiving end of the spectrometer is connected with the second collimating lens through the polarization maintaining drill rod.
4. The fully automated time-resolved detection free-space-type Mueller OCT imaging system of claim 1, wherein: the four paths of light path structures with different polarizations are respectively a horizontal polarization light path structure, a vertical polarization light path structure, a 45-degree linear polarization light path structure and a right-handed circular polarization light path structure;
the horizontally polarized light path structure is arranged on a transmission light path of the third non-polarized beam splitter and comprises a first diaphragm, a first optical switch and a first reflector which are sequentially arranged along the light path;
the vertical polarization light path structure is arranged on a reflection light path of the third non-polarization beam splitter and comprises a second diaphragm, a second optical switch, a first quarter wave plate and a second reflector which are sequentially arranged along the light path;
the 45-degree linear polarization light path structure is arranged on a reflection light path of the fourth non-polarization beam splitter, and the vertical polarization light path structure comprises a third diaphragm, a third light switch, a second quarter wave plate and a third reflector which are sequentially arranged along the light path;
the right-handed circular polarization light path structure is arranged on a transmission light path of the fourth non-polarization beam splitter, and the vertical polarization light path structure comprises a fourth diaphragm, a fourth optical switch, an eighth wave plate and a fourth reflector which are sequentially arranged along the light path.
5. The fully automated time-resolved detection free-space-type Mueller OCT imaging system of claim 1, wherein: the low-coherence broadband polarized light source adopts a super-radiation light-emitting diode or a frequency-sweeping broadband light source.
6. The fully automated time-division detected free-space-type Mueller OCT imaging system of claim 5, wherein: the wavelength of the super-luminescent diode or swept broadband light source is 1310 nm.
7. The fully automated time-resolved detection free-space-type Mueller OCT imaging system of claim 1, wherein: the bandwidth of low coherence broadband polarized light sources ranges from tens of nanometers to hundreds of nanometers.
8. The fully automated time-resolved detection free-space-type Mueller OCT imaging system of claim 1, wherein: the splitting ratio of the first non-polarizing beam splitter, the second non-polarizing beam splitter, the third non-polarizing beam splitter and the fourth non-polarizing beam splitter is 50: 50 applicable wavelength range is 1100nm ~1600nm beam splitter.
9. The fully automated time-resolved detection free-space-type Mueller OCT imaging system of claim 1, wherein: the spectrometer is a michelson interferometer or a mach-zehnder interferometer.
10. The imaging method of the fully automated time-resolved free-space-type Mueller OCT imaging system of any one of claims 1 to 9, wherein: the imaging method comprises the following steps:
(1) light emitted by the low-coherence broadband polarized light source is connected with a first collimating mirror through a polarization maintaining optical fiber and is divided into reference light and sample light through a first non-polarized beam splitter;
(2) the reference light is divided into four paths of reference light sequentially through the second non-polarization beam splitter, the third non-polarization beam splitter and the fourth non-polarization beam splitter, and the four paths of reference light returned by the reflector are respectively four different types of polarized light: horizontally, vertically, 45-degree linearly polarized light and rightly-polarized light;
(3) the optical path of the four paths of reference light is equal to that of the sample wall through adjustment, the sample light is projected to a sample to be measured through the quarter-wave plate and the half-wave plate, the quarter-wave plate and the half-wave plate are quickly rotated by utilizing a precise rotating workbench, so that four polarized lights identical to the reference wall are adjusted, and the polarization information carried by the sample light after being reflected inside the sample is coupled into a spectrometer to interfere with the four paths of light on the reference wall;
(4) the four paths of reference light and the sample light are interfered and then respectively provided with polarization information of horizontal, vertical, 45-degree and right-handed circles, the reference wall utilizes the optical switch to perform time-sharing detection on four paths of optical channels and respectively interfere with the four paths of light on the sample wall, interference spectrum signals recorded by the spectrograph are collected by the video acquisition card and then are transmitted to the computer for data processing, and 16-element information of a Mueller matrix of the sample, namely a complete polarization characteristic image of the sample, is obtained through data reconstruction.
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| CN113514400A (en) * | 2021-04-23 | 2021-10-19 | 长春理工大学 | Polarization measurement method of smoke particle Mueller matrix |
| CN113984676A (en) * | 2021-10-20 | 2022-01-28 | 南京航空航天大学 | Mueller matrix decomposition method for multiple scattering media |
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