CN112143633B - Semi-automatic microbial inoculation appearance - Google Patents
Semi-automatic microbial inoculation appearance Download PDFInfo
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- CN112143633B CN112143633B CN202011090458.2A CN202011090458A CN112143633B CN 112143633 B CN112143633 B CN 112143633B CN 202011090458 A CN202011090458 A CN 202011090458A CN 112143633 B CN112143633 B CN 112143633B
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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Abstract
The invention relates to a semi-automatic microorganism inoculation instrument, which comprises a box body, wherein the box body is internally provided with: the culture dish feeding mechanism is provided with a culture dish fixing mechanism and a sample cup fixing mechanism; the culture dish fixing mechanism is used for placing and fixing a culture dish and driving the culture dish to rotate; the streaking inoculation mechanism is arranged in the box body through a three-axis platform and is positioned above the culture dish feeding mechanism; the streaking and inoculating mechanism comprises a second motor fixedly mounted on a three-axis platform, an output end of the second motor is provided with an inoculating rod clamping jaw mechanism and a streaking rod, the inoculating rod clamping jaw mechanism and the streaking rod are fixedly connected side by side from left to right, the orientations of the inoculating rod clamping jaw mechanism and the streaking rod are opposite, and the orientations of the inoculating rod clamping jaw mechanism and the streaking rod are all perpendicular to the orientation of the output end of the second motor. The device has high functional diversity and automation degree, reduces personnel participation, and improves the safety of experimenters and the accuracy of experimental results.
Description
Technical Field
The invention belongs to the technical field of medical detection equipment, and particularly relates to a semi-automatic microorganism inoculation instrument.
Background
Among the clinical microbiological examination at present, the inoculation of the collection sample from the sample is manual completion, has not yet realized comprehensive automation, and clinical sample cup sample is sent the laboratory after, needs the manual work to open the sample bowl cover, manually picks the sample and inoculates on the culture dish, and this process needs experimenter and experimental sample direct contact, has increased the risk that the experimenter infects, and manual operation's non-standardization has also influenced the accuracy of experimental result in addition.
In the clinical microbiological examination of our country at present, because the air quality is relatively poor, the department of respiration patient is numerous, and the sputum sample becomes the sample type that accounts for the most in the microbiological examination, but because the inoculation of sputum sample needs subregion streak, has higher requirement to operating personnel, and two kinds of automatic inoculators at present can't be fine carry out the inoculation of subregion streak to the sputum sample, lead to the separation effect that the sputum sample cultivateed is not good, influence later stage's appraisal and drug sensitive result.
Therefore, the company applies for a patent (application number 201710439081.9) of a disposable multi-surface inoculation device and an inoculation method thereof in 2017, and realizes the full-automatic partition streaking inoculation of the sample, so that the inoculation is more accurate, and the inoculation efficiency is higher. But the device has single function and low automation degree.
Disclosure of Invention
The invention aims to provide a semi-automatic microorganism inoculation instrument which is high in functional diversity and automation degree, and further reduces personnel participation and improves the safety of experimenters and the accuracy of experimental results.
In order to realize the purpose, the technical scheme adopted by the invention is as follows:
the utility model provides a semi-automatic microbial inoculation appearance which characterized in that, includes box 1, install in the box 1:
the culture dish feeding mechanism 2 is provided with a culture dish fixing mechanism 240 and a sample cup fixing mechanism 250 on the culture dish feeding mechanism 2; the culture dish fixing mechanism 240 is used for placing and fixing the culture dish 7 and driving the culture dish 7 to rotate; the sample cup fixing mechanism 250 is used for placing and fixing the sample cup 8;
the streaking and inoculating mechanism 4 is arranged in the box body 1 through a three-axis platform 6 and is positioned above the culture dish feeding mechanism 2; the streaking and inoculating mechanism 4 comprises a second motor 403 fixedly mounted on a three-axis platform 6, the output end of the second motor 403 is provided with an inoculating rod clamping jaw mechanism 402 and a streaking rod 406, the inoculating rod clamping jaw mechanism 402 and the streaking rod 406 are fixedly connected side by side at the left and right, the orientation of the inoculating rod clamping jaw mechanism 402 and the orientation of the streaking rod 406 are opposite, and the orientation of the inoculating rod clamping jaw mechanism 402 and the orientation of the streaking rod 406 are both vertical to the orientation of the output end of the second motor 403; wherein the inoculation rod clamping jaw mechanism 402 is used for grabbing a sample inoculation rod 9 in a sample cup 8 and streaking a sample in a culture dish 7; the end of the scribe bar 406 is used for mounting the inoculation head 10 and for zonal scribing within the culture dish 7 by means of the inoculation head 10.
Further, the culture dish feeding mechanism 2 comprises a drawer body 201 and a guide rail 202, wherein the left side and the right side of the drawer body 201 are connected with the box body 1 through the guide rail 202 to form a drawer type push-pull connection structure.
Further, the culture dish fixing mechanism 240 comprises a tray 241 and a first motor 242, the first motor 242 is mounted on the drawer body 201, and the tray 241 is mounted at the output end of the first motor 242; the periphery of the tray 241 is uniformly provided with at least three buckles 243 for clamping and fixing the culture dish 7.
Further, the culture dish fixing mechanism 240 further comprises a second sensor 244, wherein the second sensor 244 is mounted on the drawer body 201 and used for detecting the position of the tray 241; the culture dish fixing mechanism 240 further comprises a third sensor 246, and the second sensor 244 is mounted on the drawer body 201 and positioned below the tray 241; a perspective window 245 is arranged on the tray 241 at a position opposite to the second sensor 244, and the second sensor 244 is used for detecting whether the culture dish 7 is on the tray 241.
Further, the sample cup fixing mechanism 250 comprises a supporting plate 251, a through hole matched with the outer diameter of the middle part of the sample cup 8 is formed in the center of the supporting plate 251, the middle part of the sample cup 8 penetrates through the through hole, and the upper part of the sample cup 8 is placed on the supporting plate 251; the sample cup fixing mechanism 250 further comprises a clamping fixing mechanism, and the clamping fixing mechanism comprises an L-shaped lever 252, a fulcrum fixing block 253 and a support sliding rail 255; the corners of the L-shaped lever 252 are rotatably connected with a fulcrum fixing block 253 through a rotating shaft, and the fulcrum fixing block 253 is fixedly mounted on the drawer body 201 on the side of the supporting plate 251; wherein, the upper end of the L-shaped lever 252 is provided with a hook claw and is positioned above the drawer body 201; the lower end of the L-shaped lever 252 is provided with a roller 254 and is positioned below the drawer body 201; the supporting slide track 255 is fixedly installed on the box body 1, and the supporting slide track 255 is located right below the roller 254 when the drawer body 201 is in the closed state, and is used for jacking up the roller 254.
Further, the streaking and inoculating mechanism 4 comprises a fixed bracket 401 fixedly mounted on the three-axis platform 6, the second motor 403 is fixedly mounted on the fixed bracket 401, and the inoculating rod clamping jaw mechanism 402 is arranged at the output end of the second motor 403; the side of the inoculation rod clamping jaw mechanism 402 is fixedly connected with a mounting bracket 407, a third motor 408 is fixedly mounted on the mounting bracket 407, and the scribing rod 406 is connected at the output end of the third motor 408.
Further, the fixed supports 401 are a pair and are arranged in a left-right symmetrical manner, wherein one fixed support 401 is provided with a second motor 403, and the other fixed support 401 is provided with a pair of symmetrically arranged fifth sensors 409 and an encoder 405 for detecting the positions of the inoculation rod clamping jaw mechanism 402 and the scribing rod 406; the fifth sensor 409 is an optical groove type photoelectric sensor, a semicircular contact piece 410 is arranged on one side, close to the optical groove type photoelectric sensor, of the mounting bracket 407, the semicircular contact piece 410 is concentric with the output end of the second motor 403, and the semicircular contact piece 410 is used for triggering the two optical groove type photoelectric sensors; the encoder 405 is used to monitor the rotation angle of the mounting bracket 407.
Further, the output end of the second motor 403 is connected with the inoculation rod clamping jaw mechanism 402 through a bearing, a driving block 411 is fixedly arranged at the output end of the second motor 403, two driven driving blocks 412 are arranged on one side, close to the second motor 403, of the inoculation rod clamping jaw mechanism 402, the end portion of the driving block 411 is located between the two driven driving blocks 412, and the end portion of the driving block 411 is connected with the two driven driving blocks 412 through a buffer member.
Further, the buffer members are magnetic blocks, the end portions of the driving block 411 and the two driven driving blocks 412 are provided with magnetic blocks, and the arrangement of the magnetic pole directions of the magnetic blocks should ensure that the driving block 411 and the two driven driving blocks 412 are repulsive forces.
Further, an inoculation head feeding mechanism 5 is further installed in the box body 1, and the inoculation head feeding mechanism 5 comprises a feeding mechanism support 501, a feeding pipe 502 and a waste collecting box 3; a feeding groove 503 is formed in the feeding mechanism support 501, and a discharging notch is formed in one side, facing the scribing rod 406, of the feeding groove 503 and is used for the inoculation head 10 to pass through; the feed pipe 502 is used for accommodating vertically stacked inoculating heads 10, the lower end of the feed pipe 502 is inserted into the feed tank 503, the lower end of the feed pipe 502 is provided with a discharge port 508 matched with the inoculating head 10, the discharge port 508 can only accommodate one inoculating head 10, and the discharge port 508 is opposite to a discharge groove opening; a waste material falling opening 504 is formed in the feeding mechanism support 501, the waste material collecting box 3 is positioned right below the waste material falling opening 504, and the waste material collecting box 3 is connected with the box body in a drawer-type push-pull mode; the feeding mechanism bracket 501 is further provided with a baffle 505, and the baffle 505 is positioned at one side of the discharge hole 508; a blocking groove 507 is formed in the position, opposite to the waste falling opening 504, of the blocking plate 505, and the inner diameter of the blocking groove 507 is larger than the outer diameter of the scribing rod 406 and smaller than the outer diameter of the inoculating head 10; a V-shaped guide groove 506 is arranged on the baffle 505 opposite to the discharge hole 508.
The beneficial effects of the invention are as follows: the device integrates the functions of streaking and inoculating samples, zoning and streaking, replacing an inoculating head and simultaneously processing a plurality of culture dishes, has high functional diversity and automation degree, further reduces the participation of personnel, and improves the safety of experimenters and the accuracy of experimental results.
Drawings
FIG. 1 is an overall external schematic view of a semi-automatic microorganism inoculating apparatus.
FIG. 2 is a schematic diagram showing the internal structure of a semi-automatic microorganism inoculating apparatus.
FIG. 3 is a partial schematic view (front perspective view) of the core components of a semi-automatic microorganism inoculator, including a culture dish feeding mechanism, a streaking inoculating mechanism and an inoculating head feeding mechanism.
FIG. 4 is a partial schematic view (back perspective view) of the core components of a semi-automatic microorganism inoculator.
FIG. 5 is a perspective view of the petri dish feeding mechanism.
FIG. 6 is a schematic top view of the plate feeding mechanism.
FIG. 7 is a front perspective view of the plate feeding mechanism.
FIG. 8 is a schematic view of the culture dish feeding mechanism in a reverse perspective.
FIG. 9 is a perspective view of the dish fixing mechanism.
FIG. 10 is a schematic top view of the mechanism for fixing a culture dish.
FIG. 11 is a perspective view of the specimen cup holding mechanism.
FIG. 12 is a side schematic view of the sample cup holding mechanism.
FIG. 13 is a front perspective view of the streaking inoculation mechanism.
FIG. 14 is a rear perspective view of the streaking inoculation mechanism.
FIG. 15 is a partial schematic view at the inoculation lever gripper mechanism in the streaking inoculation mechanism.
FIG. 16 is a partial schematic view of the streaking mechanism at the streaking bar.
FIG. 17 is a front perspective view of the feeding mechanism of the inoculating head.
FIG. 18 is a partial schematic view of the feed mechanism of the inoculating head at the feed tank.
In the figure: 1-a box body is arranged in the box body,
2-culture dish feeding mechanism, 201-drawer body, 202-guide rail, 203-first sensor,
240-culture dish fixing mechanism, 241-tray, 242-first motor, 243-buckle, 244-second sensor, 245-perspective window, 246-third sensor,
250-sample cup fixing mechanism, 251-supporting plate, 252-L-shaped lever, 253-fulcrum fixing block, 254-roller, 255-supporting slide rail, 256-fourth sensor,
3-a waste material collecting box, wherein,
4-a marking inoculation mechanism, 401-a fixed bracket, 402-an inoculation rod clamping jaw mechanism, 403-a second motor, 404-a coupler, 405-an encoder, 406-a marking rod, 407-a mounting bracket, 408-a third motor, 409-a fifth sensor, 410-a semicircular contact piece, 411-a driving block and 412-a driven driving block,
5-inoculating head feeding mechanism, 501-feeding mechanism support, 502-feeding pipe, 503-feeding groove, 504-waste falling opening, 505-baffle, 506-guide groove, 507-blocking groove, 508-discharging opening,
6-a three-axis platform, wherein,
7-a culture dish for culturing the bacteria,
8-a sample cup, wherein the sample cup is arranged in the sample cup,
9-sample inoculation pole
10-inoculation head.
Detailed Description
In order to better understand the present invention, the following embodiments are further described.
Referring to fig. 1, 2, 3 and 4, a semi-automatic microorganism inoculation instrument comprises a box body 1, wherein a culture dish feeding mechanism 2, a streak inoculation mechanism 4 and an inoculation head feeding mechanism 5 are installed in the box body 1.
As shown in fig. 5, 6, 7 and 8, the culture dish feeding mechanism 2 and the box 1 are in a drawer-type push-pull connection structure. The culture dish feeding mechanism 2 comprises a drawer body 201 and guide rails 202, wherein the left side and the right side of the drawer body 201 are connected with the box body 1 through the guide rails 202 to form a drawer type push-pull connecting structure. Push-pull type drawer structure, convenient change culture dish 7 and sample cup 8 also can make whole streak inoculation process all be located box 1 airtight going on.
As shown in fig. 5 and 6, a first sensor 203 is further disposed on the box body 1 at the tail of the drawer body 201, and the first sensor 203 is an optical slot type photoelectric sensor and includes a contact. Wherein light slot formula photoelectric sensor installs on box 1, and the contact is installed at drawer body 201 afterbody, and when drawer body 201 moved to the innermost, was in the closed condition, the contact then along with drawer body 201 motion, inserted in the light slot for detect drawer body 201's position.
As shown in fig. 5, 6 and 7, three culture dish fixing mechanisms 240 and a sample cup fixing mechanism 250 are arranged on the drawer body 201, and are arranged in a rectangular shape.
As shown in fig. 7, 9 and 10, the culture dish fixing mechanism 240 includes a tray 241 and a first motor 242, the first motor 242 is mounted on the drawer body 201 and located below the drawer body 201, and an output end of the first motor 242 penetrates through the drawer body 201; the tray 241 is located on the drawer body 201 and is installed at an output end of the first motor 242, and the first motor 242 is used for driving the tray 241 to rotate. At least three buckles 243 are uniformly arranged on the periphery of the tray 241 and used for clamping the fixed culture dish 7.
The culture dish fixing mechanism 240 further comprises a second sensor 244, and the second sensor 244 is an optical groove type photoelectric sensor and comprises a contact piece. As shown in fig. 9, the optical groove type photoelectric sensor is installed on the drawer body 201, the contact piece is installed at the bottom of the tray 241, and when the tray 241 rotates, the contact piece passes through the optical groove of the optical groove type photoelectric sensor to detect the position of the tray 241.
The culture dish fixing mechanism 240 further comprises a third sensor 246, the third sensor 246 is an infrared reflection sensor, and the infrared reflection sensor is mounted on the drawer body 201 and located below the tray 241; a perspective window 245 is arranged on the tray 241 opposite to the second sensor 244, and the infrared reflection sensor is used for detecting whether the culture dish 7 is on the tray 241.
As shown in fig. 11 and 12, the sample cup fixing mechanism 250 includes a supporting plate 251, and the drawer body 201 has a hole, where the supporting plate 251 is installed. The center of the supporting plate 251 is provided with a through hole matched with the outer diameter of the middle part of the sample cup 8, the middle part of the sample cup 8 passes through the through hole and is positioned in the hole, and the upper part of the sample cup 8 is placed on the supporting plate 251.
The sample cup fixing mechanism 250 further comprises two clamping and fixing mechanisms symmetrically arranged on two sides of the sample cup 8 for fixing the sample cup 8, so that the sample inoculation rod 9 can be pulled out conveniently. The clamping and fixing mechanism comprises an L-shaped lever 252, a fulcrum fixing block 253 and a supporting slide rail 255; the corners of the L-shaped lever 252 are rotatably connected with a fulcrum fixing block 253 through a rotating shaft, and the fulcrum fixing block 253 is fixedly installed at a hole; wherein, the upper end of the L-shaped lever 252 is provided with a hook claw and is positioned above the drawer body 201; the lower end of the L-shaped lever 252 is provided with a roller 254 and is positioned below the drawer body 201; the supporting slide track 255 is fixedly installed on the box body 1, and the supporting slide track 255 is located right below the roller 254 when the drawer body 201 is in a closed state and is used for jacking up the roller 254; when the drawer body 201 is in a closed state, the roller 254 falls on the supporting slide track 255, the roller 254 is jacked up, and the hook claw at the upper end of the L-shaped lever 252 hooks inwards to press the sample cup 8 to fix the sample cup through the lever principle; when the drawer body 201 is in an open state, the roller 254 falls on the box body 1 or hangs in the air, the roller 254 falls, the height of the roller 254 is lower than that of the roller 254 falling on the supporting slide track 255, and under the action of the lever principle, the hook claw at the upper end of the L-shaped lever 252 is opened outwards to release the sample cup 8.
A fourth sensor 256 is further included, and the fourth sensor 256 is an infrared reflection sensor disposed at a lower portion of the tray 241 for detecting the presence or absence of the sample cup 8.
The sample cup 8 is preferably a "disposable microorganism sample inoculation cup" manufactured by the company, application No.: CN201710439061.1.
Referring to figures 4, 13, 14, 15 and 16, the streaking inoculating device 4 is mounted in the housing 1 above the dish feeding device 2 by a three-axis platform 6.
The streaking and inoculating mechanism 4 comprises a pair of fixed supports 401 symmetrically arranged on the three-axis platform 6, wherein one fixed support 401 is provided with a second motor 403, and the other fixed support 401 is provided with a pair of symmetrically arranged fifth sensors 409 and an encoder 405.
An output end of the second motor 403 (the output end mainly comprises a motor output shaft, a coupling 404 and a rotating shaft) is provided with an inoculation rod clamping jaw mechanism 402 and a scribing rod 406, the inoculation rod clamping jaw mechanism 402 and the scribing rod 406 are fixedly connected side by side from left to right, the orientations of the inoculation rod clamping jaw mechanism 402 and the scribing rod 406 are opposite, and the orientations of the inoculation rod clamping jaw mechanism 402 and the scribing rod 406 are both vertical to the orientation of the output end of the second motor 403; wherein the inoculation rod clamping jaw mechanism 402 is used for grabbing a sample inoculation rod 9 in a sample cup 8 and streaking a sample in a culture dish 7; the end of the scribe bar 406 is used for mounting the inoculation head 10 and for zonal scribing within the culture dish 7 by means of the inoculation head 10.
The inoculation rod clamping mechanism 402 is arranged at the output end of the second motor 403; the side surface of the inoculation rod clamping jaw mechanism 402 is fixedly connected with a mounting bracket 407, a third motor 408 is fixedly mounted on the mounting bracket 407, and the scribing rod 406 is connected to the output end of the third motor 408. The inoculating head 10 in the present embodiment is preferably a four-sided inoculating rod in the patent application No. 2017104390819.
The output of second motor 403 with inoculation pole gripper mechanism 402 passes through the bearing and connects, the fixed initiative drive block 411 that is equipped with on the output of second motor 403, one side that leans on second motor 403 on the inoculation pole gripper mechanism 402 is equipped with two driven drive blocks 412, the tip of initiative drive block 411 is located between two driven drive blocks 412, all be connected through the bolster between the tip of initiative drive block 411 and two driven drive blocks 412.
The buffer member may be an elastic member, such as a spring, a disc spring, a spring sheet, elastic rubber, or the like.
The buffer is preferably a magnetic block, the end of the driving block 411 and the two driven driving blocks 412 are provided with a magnetic block, and the magnetic pole direction of each magnetic block should be arranged to ensure that the driving block 411 and the two driven driving blocks 412 are repulsive force. For example: the N pole of the magnetic block a on the driven driving block 412 faces the driving block 411, two magnetic blocks b are arranged on the driving block 411, the N poles of the two magnetic blocks b face outwards and face the driven driving block 412 on the same side, and magnetic poles with similar functions can be arranged. The active drive block 411 drives the inoculation rod clamping jaw mechanism 402 and the scribing rod 406 to rotate through a repulsive force, and provides buffering to prevent hard contact when the sample inoculation rod 9 or the inoculation head 10 contacts the culture dish 7; and the driving form of the repulsive force can also prevent the elastic member such as a spring from shaking back and forth.
The fifth sensor 409 is an optical groove type photoelectric sensor, a semicircular contact piece 410 is arranged on one side of the mounting bracket 407 close to the optical groove type photoelectric sensor, the semicircular contact piece 410 is concentric with the output end of the second motor 403, and the semicircular contact piece 410 is used for triggering the two optical groove type photoelectric sensors. The midline of the semicircular wafer 410 is towards the side of the sample inoculation rod 9, and the positions of the clamping jaw mechanism 402 and the scribing rod 406 can be determined by the types of the different optical slot type photoelectric sensors, for example: when the semicircular contact piece 410 triggers one of the optical slot type photoelectric sensors, it indicates that the side of the sample inoculation rod 9 facing the optical slot type photoelectric sensor.
The encoder 405 is used to monitor the angle of rotation of the mounting bracket 407, thereby achieving the purpose of monitoring the angle of rotation of the inoculation bar gripper mechanism 402 and the score bar 406. For adjusting the amount of rotation of the second motor 403 with more angle of rotation fed back by the encoder 405.
As shown in fig. 1, 3, 17 and 18, the inoculating head feeding mechanism 5 comprises a feeding mechanism bracket 501, a feeding pipe 502 and a waste collecting box 3;
the middle part of the feeding mechanism support 501 is provided with a waste falling opening 504, the waste collecting box 3 is positioned under the waste falling opening 504, and the waste collecting box 3 is connected with the box body in a drawer type push-pull mode.
The feeding mechanism support 501 is provided with two feeding grooves 503, and the two feeding grooves 503 are symmetrically arranged on two sides of the waste material falling opening 504; a discharging notch is formed in one side, facing the scribing rod 406, of the feeding groove 503 and used for the inoculation head 10 to pass through; the feed pipe 502 is used for accommodating vertically stacked inoculating heads 10, the lower end of the feed pipe 502 is inserted into the feed groove 503, the lower end of the feed pipe 502 is provided with a discharge port 508 matched with the inoculating head 10, the discharge port 508 can only accommodate one inoculating head 10, and the discharge port 508 is opposite to the discharge groove opening.
A baffle 505 is arranged on the feeding mechanism bracket 501, and the baffle 505 is positioned at one side of the discharge hole 508; a blocking groove 507 is arranged on the baffle 505 opposite to the waste falling opening 504, and the inner diameter of the blocking groove 507 is larger than the outer diameter of the scribing rod 406 and smaller than the outer diameter of the inoculating head 10. A V-shaped guide groove 506 is arranged on the baffle 505 opposite to the discharge hole 508.
The working principle and the process of the semi-automatic microorganism inoculation instrument are as follows:
(1) The culture dish feeding mechanism 2 is pulled out, the culture dish 7 and the sample cup 8 are placed on the drawer body 201 and pushed into the culture dish feeding mechanism 2, and the first sensor 203 confirms that the culture dish feeding mechanism 2 is pushed in place; a feed tube 502 for filling the inoculating head 10 is inserted into the feed tank 503. Wherein the sample cup 8 is secured under the action of the L-shaped lever 252 and the support slide 255.
(2) The device is started, the inoculation rod clamping jaw mechanism 402 grabs the sample inoculation rod 9 in the sample cup 8 under the synergistic action of the three-axis platform 6 and the second motor 403, moves to the culture dish 7, and rotates the culture dish to perform streak inoculation on the sample.
The triaxial platform 6 and the second motor 403 move the scribing rod 406 to fall into the guide groove 506, then the discharging port 508 is inserted into the inoculating head 10, the inoculating head is taken out, and then the culture dish for streaking and inoculating the sample is subjected to the partition streaking operation. By the rotation of the third motor 408, three surfaces of the inoculating head 10 are used for three dishes 7, respectively.
After the inoculating head 10 is used up, the three-axis platform 6 and the second motor 403 move the scribing rod 406 to the blocking groove 507, and then pull back the scribing rod 406 to pull out the inoculating head 10, and drop the inoculating head into the waste collecting box 3.
(3) The three culture dishes 7 which have been inoculated are taken out by withdrawing the culture dish feeding mechanism 2, and the operation is repeated by replacing the three culture dishes 7 with new ones. When the inoculating head 10 is used up in the feed tube 502, a new feed tube 502 can be replaced.
The above description is only an application example of the present invention, and certainly, the present invention should not be limited by this application, and therefore, the present invention is still within the protection scope of the present invention by equivalent changes made in the claims of the present invention.
Claims (10)
1. The utility model provides a semi-automatic microbial inoculation appearance which characterized in that, includes box (1), install in box (1):
the culture dish feeding mechanism (2) is provided with a culture dish fixing mechanism (240) and a sample cup fixing mechanism (250); the culture dish fixing mechanism (240) is used for placing and fixing a culture dish (7) and driving the culture dish (7) to rotate; the sample cup fixing mechanism (250) is used for placing and fixing a sample cup (8);
the streaking inoculation mechanism (4) is arranged in the box body (1) through a three-axis platform (6) and is positioned above the culture dish feeding mechanism (2); the streaking and inoculating mechanism (4) comprises a second motor (403) fixedly mounted on a three-axis platform (6), the output end of the second motor (403) is provided with an inoculating rod clamping jaw mechanism (402) and a streaking rod (406), the inoculating rod clamping jaw mechanism (402) and the streaking rod (406) are fixedly connected side by side from left to right, the inoculating rod clamping jaw mechanism (402) and the streaking rod (406) face opposite directions, and the inoculating rod clamping jaw mechanism (402) and the streaking rod (406) face towards directions which are perpendicular to the output end of the second motor (403); wherein the inoculation rod clamping jaw mechanism (402) is used for grabbing a sample inoculation rod (9) in a sample cup (8) and streaking a sample in a culture dish (7); the end of the scribing rod (406) is used for installing the inoculation head (10) and is used for scribing in the culture dish (7) in a partition mode through the inoculation head (10).
2. The semi-automatic microorganism inoculation instrument according to claim 1, wherein the culture dish feeding mechanism (2) comprises a drawer body (201) and a guide rail (202), and the left side and the right side of the drawer body (201) are connected with the box body (1) through the guide rail (202) to form a drawer type push-pull connection structure.
3. A semi-automatic microorganism inoculating instrument according to claim 2, wherein the culture dish fixing mechanism (240) comprises a tray (241) and a first motor (242), the first motor (242) is installed on the drawer body (201), the tray (241) is installed at the output end of the first motor (242); the tray (241) is evenly provided with at least three buckles (243) around for clamping the fixed culture dish (7).
4. A semi-automatic micro-organism inoculating device according to claim 3, wherein the culture dish fixing mechanism (240) further comprises a second sensor (244), the second sensor (244) is mounted on the drawer body (201) for detecting the position of the tray (241); the culture dish fixing mechanism (240) further comprises a third sensor (246), and the second sensor (244) is installed on the drawer body (201) and is positioned below the tray (241); a perspective window (245) is arranged on the tray (241) opposite to the second sensor (244), and the second sensor (244) is used for detecting whether a culture dish (7) is arranged on the tray (241).
5. A semi-automatic microorganism inoculating instrument according to claim 2, characterized in that the sample cup fixing mechanism (250) comprises a supporting plate (251), the center of the supporting plate (251) is provided with a through hole matching with the outer diameter of the middle part of the sample cup (8), the middle part of the sample cup (8) passes through the through hole, and the upper part of the sample cup (8) is rested on the supporting plate (251); the sample cup fixing mechanism (250) further comprises a clamping fixing mechanism, and the clamping fixing mechanism comprises an L-shaped lever (252), a fulcrum fixing block (253) and a supporting slide rail (255); the corners of the L-shaped lever (252) are rotatably connected with a fulcrum fixing block (253) through a rotating shaft, and the fulcrum fixing block (253) is fixedly arranged on a drawer body (201) on the side of the supporting plate (251); the upper end of the L-shaped lever (252) is provided with a hook claw and is positioned above the drawer body (201); the lower end of the L-shaped lever (252) is provided with a roller (254) and is positioned below the drawer body (201); the supporting sliding rail (255) is fixedly installed on the box body (1), and the supporting sliding rail (255) is located right below the roller (254) of the drawer body (201) in the closing state and used for jacking the roller (254).
6. A semi-automatic microorganism inoculating instrument according to claim 1, wherein the streaking inoculating mechanism (4) comprises a fixing bracket (401) fixedly mounted on a three-axis platform (6), the second motor (403) is fixedly mounted on the fixing bracket (401), and the inoculating rod clamping jaw mechanism (402) is arranged at the output end of the second motor (403); the side surface of the inoculation rod clamping jaw mechanism (402) is fixedly connected with a mounting bracket (407), a third motor (408) is fixedly mounted on the mounting bracket (407), and the scribing rod (406) is connected to the output end of the third motor (408).
7. A semi-automatic microorganism inoculating instrument as claimed in claim 6, wherein the fixed supports (401) are a pair, and are arranged in bilateral symmetry, one of the fixed supports (401) is provided with the second motor (403), and the other fixed support (401) is provided with a pair of symmetrically arranged fifth sensors (409) and an encoder (405) for detecting the positions of the inoculating rod gripper mechanism (402) and the scribing rod (406); the fifth sensor (409) is an optical groove type photoelectric sensor, a semicircular contact piece (410) is arranged on one side, close to the optical groove type photoelectric sensor, of the mounting support (407), the semicircular contact piece (410) and the output end of the second motor (403) are concentric, and the semicircular contact piece (410) is used for triggering the two optical groove type photoelectric sensors; the encoder (405) is used for monitoring the rotation angle of the mounting bracket (407).
8. The semi-automatic microorganism inoculation instrument according to claim 6, wherein the output end of the second motor (403) is connected with the inoculation rod clamping jaw mechanism (402) through a bearing, a driving block (411) is fixedly arranged on the output end of the second motor (403), two driven driving blocks (412) are arranged on one side, close to the second motor (403), of the inoculation rod clamping jaw mechanism (402), the end portion of the driving block (411) is located between the two driven driving blocks (412), and the end portion of the driving block (411) is connected with the two driven driving blocks (412) through a buffer.
9. A semi-automatic microorganism inoculation instrument according to claim 8, characterized in that the buffer members are magnetic blocks, the ends of the driving block (411) and the two driven blocks (412) are provided with magnetic blocks, and the magnetic poles of the magnetic blocks are arranged in a direction that ensures that the driving block (411) and the two driven blocks (412) are repulsive.
10. A semi-automatic microorganism inoculation instrument according to claim 1, characterized in that an inoculation head feeding mechanism (5) is further installed in the box body (1), and the inoculation head feeding mechanism (5) comprises a feeding mechanism bracket (501), a feeding pipe (502) and a waste collecting box (3); a feeding groove (503) is formed in the feeding mechanism support (501), and a discharging notch is formed in one side, facing the scribing rod (406), of the feeding groove (503) and used for the inoculation head (10) to pass through; the feeding pipe (502) is used for accommodating vertically stacked inoculating heads (10), the lower end of the feeding pipe (502) is inserted into the feeding groove (503), the lower end of the feeding pipe (502) is provided with a discharge port (508) matched with the inoculating heads (10), the discharge port (508) can only accommodate one inoculating head (10) to pass through, and the discharge port (508) is over against the opening of the discharge groove; a waste material falling opening (504) is formed in the feeding mechanism support (501), the waste material collecting box (3) is located right below the waste material falling opening (504), and the waste material collecting box (3) is connected with the box body in a drawer type push-pull mode; the feeding mechanism support (501) is also provided with a baffle (505), and the baffle (505) is positioned on one side of the discharge hole (508); a blocking groove (507) is formed in the position, right opposite to the waste falling opening (504), of the blocking plate (505), and the inner diameter of the blocking groove (507) is larger than the outer diameter of the scribing rod (406) and smaller than the outer diameter of the inoculating head (10); and a V-shaped guide groove (506) is arranged on the baffle (505) opposite to the discharge hole (508).
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