CN112126600A - Microbial continuous culture water quality purification microbial inoculum and preparation method thereof - Google Patents
Microbial continuous culture water quality purification microbial inoculum and preparation method thereof Download PDFInfo
- Publication number
- CN112126600A CN112126600A CN202010991410.2A CN202010991410A CN112126600A CN 112126600 A CN112126600 A CN 112126600A CN 202010991410 A CN202010991410 A CN 202010991410A CN 112126600 A CN112126600 A CN 112126600A
- Authority
- CN
- China
- Prior art keywords
- water quality
- parts
- microbial inoculum
- bacteria
- bacillus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/101—Sulfur compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/166—Nitrites
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Mycology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biochemistry (AREA)
- Virology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Botany (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a water quality purifying microbial inoculum for continuous culture of microorganisms and a preparation method thereof, wherein the water quality purifying microbial inoculum comprises lactic acid bacteria, photosynthetic bacteria, nitrobacteria, bacillus, carbonated syrup and beef tallow, can rapidly reduce harmful bacteria in a water body, continuously increase the photosynthetic decomposition of water quality for culturing the lactic acid bacteria, saccharomycetes and the photosynthetic bacteria, rapidly act on bottom mud, reduce ammonia nitrogen, hydrogen sulfide and nitrite, stabilize the pH value, improve the photosynthetic capacity, adjust the water color, improve the water quality, flocculate and precipitate heavy metal ions, degrade toxic and harmful components in chemical raw materials and pesticides, provide sustained-release and better oxygenation effects, the beef tallow is added into the water quality purifying microbial inoculum for providing the propagation and stabilization of beneficial microbial communities in the water body, and the carbonated syrup with 85 percent of hammer degree is additionally added, thereby providing the self-splitting nutrition of the nitrobacteria and the photosynthetic bacteria in the water body and promoting the propagation of the nitrobacteria and the photosynthetic bacteria, after the water quality purification microbial inoculum is added, the flora balance can be kept for a long time, and the purification function can be continuously exerted.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to a microbial continuous culture water quality purification microbial inoculum and a preparation method thereof.
Background
In the process of high-density aquaculture, in order to make the problem of water pollution increasingly prominent during rapid and high-yield production, the ecological system of the water quality is not only related to the input-output ratio of aquaculture, but also is a key point for sustainable development of aquaculture, and in the aspects of water pollution and eutrophic water treatment, a plurality of technologies and measures are provided, but all have certain limitations, a plurality of complex microbial inoculum is provided in the prior art for purifying the water quality, but the existing complex microbial inoculum has the following defects:
1. the microbial bacteria have insufficient activity, and a large amount of microbial inoculum needs to be continuously added for a long time
2. The existing complex microbial inoculum has high use cost and unsustainability.
Disclosure of Invention
The invention aims to provide a water quality purifying microbial inoculum for microbial continuous culture and a preparation method thereof.
The technical scheme adopted by the invention is as follows:
a microbial continuous culture water quality purification microbial inoculum comprises lactic acid bacteria, photosynthetic bacteria, nitrobacteria, bacillus, sugar water carbonate and beef tallow.
Furthermore, the water quality purification microbial inoculum comprises, by weight, 15-35 parts of lactic acid bacteria, 5-10 parts of photosynthetic bacteria, 5-10 parts of nitrobacteria, 5-10 parts of bacillus, 20-30 parts of carbonated sugar water and 1-5 parts of beef tallow.
Furthermore, the water quality purification microbial inoculum comprises 15 parts by weight of lactic acid bacteria, 5 parts by weight of photosynthetic bacteria, 5 parts by weight of nitrobacteria, 5 parts by weight of bacillus, 25 parts by weight of carbonated sugar water and 5 parts by weight of beef tallow.
Furthermore, the number of the lactic acid bacteria is 10-20 hundred million/g, the number of the photosynthetic bacteria is 1-5 hundred million/g, the number of the nitrifying bacteria is 1-5 hundred million/g, and the number of the bacillus is 20-30 hundred million/g.
Further, the carbonated sugar water is 85% of the carbonated sugar water in bulk.
Further, the lactic acid bacteria are lactobacillus acidophilus, lactobacillus plantarum and saccharomyces cerevisiae mixed bacteria.
Further, the bacillus is a bacillus subtilis and bacillus stearothermophilus mixed bacterium.
Further, the pH value of the water quality purification microbial inoculum is 3.8.
The invention also provides a preparation method of the microbial continuous culture water quality purification microbial inoculum,
respectively performing single pure culture on photosynthetic bacteria and nitrobacteria by adopting a closed cover, performing static culture at the temperature of 20-35 ℃ for 7-14 days, slightly opening the cover every day, and shaking and mixing for 1-4 times to complete strain culture;
step two, inoculating lactobacillus acidophilus, lactobacillus plantarum and beer yeast into a culture solution during lactobacillus compound culture, wherein the inoculation ratio of each strain is 1: 1000, standing and culturing at 20-35 ℃, stirring at least twice a day, and culturing for 15-30 days each time until the pH value of the culture solution is 2.8-3.0;
inoculating bacillus subtilis and bacillus stearothermophilus into a beef tallow culture medium during bacillus composite culture, wherein the inoculation ratio of each strain is 1: 1000, and performing static culture at the temperature of 20-35 ℃ for 3-5 days;
and step four, mixing the photosynthetic bacteria and the nitrifying bacteria cultured in the step one, the lactic acid bacteria cultured in the step two and the bacillus cultured in the step three, adjusting the pH value to 3.8 by using phosphoric acid, and adding the heated and mixed beef tallow and the carbonic acid sugar water with the hammer degree of 85%.
The invention has the following beneficial effects: the continuous microorganism culture technology provided by the application mainly acts on cost reduction, fast reduces harmful bacteria in a water body, continuously increases cultured lactic acid bacteria, saccharomycetes and photosynthetic bacteria for water quality photosynthetic decomposition, fast acts on bottom mud, reduces ammonia nitrogen, hydrogen sulfide, nitrite and stable PH value, improves photosynthesis capacity, adjusts water color, improves water quality, flocculates and precipitates heavy metal ions, degrades toxic and harmful components in chemical raw materials and pesticides, realizes slow release and supply and better oxygenation effect, can fast proliferate bacillus by adding butter into a basic culture medium serving as a culture medium of bacillus, greatly increases the number of effective strains in unit weight, adds butter into a water quality purification microbial inoculum for providing propagation and stability of beneficial flora in the water body, and additionally adds 85% of brix carbonated syrup, thereby providing self-splitting nutrition of nitrobacteria and photosynthetic bacteria in the water body, promote the reproduction of nitrobacteria and photosynthetic bacteria, and ensure that the water quality purification microbial inoculum can keep the flora balance for a long time after being added, thereby continuously playing the purification function.
Detailed Description
The present invention will be further described in detail with reference to examples and effect examples, but the scope of the present invention is not limited thereto.
Example 1:
respectively performing single pure culture on photosynthetic bacteria and nitrobacteria by adopting a closed cover, standing and culturing for 7 days at the temperature of 20-35 ℃, slightly opening the cover every day, and shaking and mixing for 3 times to complete strain culture;
step two, inoculating lactobacillus acidophilus, lactobacillus plantarum and beer yeast into an MRS culture solution during lactobacillus compound culture, wherein the inoculation ratio of each strain is 1: 1000, standing and culturing at 35 ℃, stirring at least twice a day, and culturing for 15 days for 15 minutes each time until the pH value of the culture solution is between 2.8 and 3.0;
inoculating bacillus subtilis and bacillus stearothermophilus into a broth agar culture medium containing 10g of beef tallow in a ratio of 1: 1000 during bacillus composite culture, and performing static culture at 35 ℃ for 3 days;
and step four, mixing 5 parts of photosynthetic bacteria, 5 parts of nitrobacteria, 15 parts of lactic acid bacteria and 5 parts of bacillus, adjusting the pH value to 3.8, and adding 5 parts of beef tallow and 25 parts of 85% Bridgman solution.
Example 2:
respectively performing single pure culture on photosynthetic bacteria and nitrobacteria by adopting a closed cover, standing and culturing for 7 days at the temperature of 20-35 ℃, slightly opening the cover every day, and shaking and mixing for 3 times to complete strain culture;
step two, inoculating lactobacillus acidophilus, lactobacillus plantarum and beer yeast into an MRS culture solution during lactobacillus compound culture, wherein the inoculation ratio of each strain is 1: 1000, standing and culturing at 35 ℃, stirring at least twice a day, and culturing for 15 days for 15 minutes each time until the pH value of the culture solution is between 2.8 and 3.0;
inoculating bacillus subtilis and bacillus stearothermophilus into a broth agar culture medium containing 10g of beef tallow in a ratio of 1: 1000 during bacillus composite culture, and performing static culture at 35 ℃ for 3 days;
and step four, mixing 5 parts of photosynthetic bacteria, 5 parts of nitrobacteria, 15 parts of lactic acid bacteria and 5 parts of bacillus, adjusting the pH value to 3.8, and adding 5 parts of beef tallow and 20 parts of 85% Bridgman solution.
Example 3: preparation of SF/Hb oxygen-containing hydrogel
Respectively performing single pure culture on photosynthetic bacteria and nitrobacteria by adopting a closed cover, standing and culturing for 7 days at the temperature of 20-35 ℃, slightly opening the cover every day, and shaking and mixing for 3 times to complete strain culture;
step two, inoculating lactobacillus acidophilus, lactobacillus plantarum and beer yeast into an MRS culture solution during lactobacillus compound culture, wherein the inoculation ratio of each strain is 1: 1000, standing and culturing at 35 ℃, stirring at least twice a day, and culturing for 15 days for 15 minutes each time until the pH value of the culture solution is between 2.8 and 3.0;
inoculating bacillus subtilis and bacillus stearothermophilus into a broth agar culture medium containing 10g of beef tallow in a ratio of 1: 1000 during bacillus composite culture, and performing static culture at 35 ℃ for 3 days;
and step four, mixing 5 parts of photosynthetic bacteria, 5 parts of nitrobacteria, 15 parts of lactic acid bacteria and 5 parts of bacillus, adjusting the pH value to 3.8, and adding 1 part of beef tallow and 25 parts of 85% Bridgman solution.
Example 4:
respectively performing single pure culture on photosynthetic bacteria and nitrobacteria by adopting a closed cover, standing and culturing for 7 days at the temperature of 20-35 ℃, slightly opening the cover every day, and shaking and mixing for 3 times to complete strain culture;
step two, inoculating lactobacillus acidophilus, lactobacillus plantarum and beer yeast into an MRS culture solution during lactobacillus compound culture, wherein the inoculation ratio of each strain is 1: 1000, standing and culturing at 35 ℃, stirring at least twice a day, and culturing for 15 days for 15 minutes each time until the pH value of the culture solution is between 2.8 and 3.0;
inoculating bacillus subtilis and bacillus stearothermophilus into a broth agar culture medium containing 10g of beef tallow in a ratio of 1: 1000 during bacillus composite culture, and performing static culture at 35 ℃ for 3 days;
and step four, mixing 10 parts of photosynthetic bacteria, 10 parts of nitrobacteria, 35 parts of lactic acid bacteria and 10 parts of bacillus, adjusting the pH value to 3.8, and adding 5 parts of beef tallow and 25 parts of 85% Bridgman solution.
Comparative example 1: in contrast to the examples, no tallow was added.
Comparative example 2: in contrast to the examples, no sugar water carbonate was added.
Comparative example 3: unlike the examples, no tallow and no sugar water carbonate were added.
Comparative example 4: unlike the examples, 20 parts of the carbonated water having a brix of 50% was replaced with the carbonated water.
1000ml of culture seawater and fresh water with COD and BOD both greater than 1000ppm are taken, the initial COD and BOD values of the water sample are detected, 1.5g of the water quality purification microbial inoculum prepared in the examples 1-4 and the comparative examples 1-4 is added, the COD and BOD values of the water sample after 3 hours, 6 hours, 12 hours and 3 days are respectively detected, and the water quality degradation rate is calculated, wherein the results are as follows:
through the experiment, the method has the advantages that the beef tallow and the 85% brix carbonated syrup are added to promote the reproduction and the stability of floras, so that the water quality purification microbial inoculum can keep the floras balanced for a long time after being added, the purification function is continuously exerted, and the purification capacity is greatly improved.
The water quality purification microbial inoculum prepared in examples 1 to 4 and comparative examples 1 to 4 was added to different small-sized shrimp fry ponds in an amount of 0.2g per liter, and the average weight of 20 shrimp fries was measured after 2 months, and the results showed that the average weight of shrimp fries in the pond in which the water quality purification microbial inoculum of the examples of the present invention was placed was generally higher than the average weight of shrimp fries in the pond to which no beef tallow or carbonated sugar water was added in proportion, because the water quality purification microbial inoculum decomposed macromolecular biomass in water and decomposed into micromolecular protein and sugar water compounds to promote the growth of shrimp fries, while the present application maintained the flora in an active state for a long period by adding beef tallow or carbonated sugar water to decompose more substances to supply nutrition.
Finally, it must be said here that: the above embodiments are only used for further detailed description of the technical solutions of the present invention, and should not be understood as limiting the scope of the present invention, and the insubstantial modifications and adaptations made by those skilled in the art according to the above descriptions of the present invention are within the scope of the present invention.
Claims (9)
1. A microbial continuous culture water quality purification microbial inoculum is characterized in that: the water quality purification microbial inoculum comprises lactic acid bacteria, photosynthetic bacteria, nitrobacteria, bacillus, carbonated sugar water and beef tallow.
2. The microbial continuous culture water quality purification microbial inoculum according to claim 1, which is characterized in that: the composition comprises, by weight, 15-35 parts of lactic acid bacteria, 5-10 parts of photosynthetic bacteria, 5-10 parts of nitrobacteria, 5-10 parts of bacillus, 20-30 parts of carbonated sugar water and 1-5 parts of beef tallow.
3. The microbial continuous culture water quality purification microbial inoculum according to claim 2, which is characterized in that: the nutrient solution comprises the following components, by weight, 15 parts of lactic acid bacteria, 5 parts of photosynthetic bacteria, 5 parts of nitrobacteria, 5 parts of bacillus, 25 parts of carbonated sugar water and 5 parts of beef tallow.
4. The microbial continuous culture water quality purification microbial inoculum according to claim 1, which is characterized in that: the number of the lactic acid bacteria is 10-20 hundred million/g, the number of the photosynthetic bacteria is 1-5 hundred million/g, the number of the nitrifying bacteria is 1-5 hundred million/g, and the number of the bacillus is 20-30 hundred million/g.
5. The microbial continuous culture water purification microbial inoculum according to claim 1, 2, 3 or 4, which is characterized in that: the carbonated sugar water is 85% of the brix.
6. The microbial continuous culture water quality purification microbial inoculum according to claim 1, which is characterized in that: the lactobacillus is mixed bacteria of Lactobacillus acidophilus, Lactobacillus plantarum and Saccharomyces cerevisiae.
7. The microbial continuous culture water quality purification microbial inoculum according to claim 1, which is characterized in that: the bacillus is a mixed bacterium of bacillus subtilis and bacillus stearothermophilus.
8. The microbial continuous culture water purification microbial inoculum according to claim 1, 2, 3 or 4, which is characterized in that: the pH value of the water quality purification microbial inoculum is 3.8.
9. A preparation method of a microbial continuous culture water purification microbial inoculum is characterized by comprising the following steps:
respectively performing single pure culture on photosynthetic bacteria and bacillus subtilis by adopting a closed cover, performing static culture at the temperature of 20-35 ℃ for 7-14 days, slightly opening the cover every day, and shaking and mixing for 1-4 times to complete strain culture;
step two, inoculating lactobacillus acidophilus, lactobacillus plantarum and beer yeast into a culture solution during lactobacillus compound culture, wherein the inoculation ratio of each strain is 1: 1000, standing and culturing at 20-35 ℃, stirring at least twice a day, and culturing for 15-30 days each time until the pH value of the culture solution is 2.8-3.0;
and step three, mixing the photosynthetic bacteria and the bacillus subtilis cultured in the step one with the lactic acid bacteria cultured in the step two, adjusting the pH value to 3.8 by using phosphoric acid, and adding the heated and mixed beef tallow and the carbonic acid sugar water with the hammer degree of 85%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010991410.2A CN112126600A (en) | 2020-09-18 | 2020-09-18 | Microbial continuous culture water quality purification microbial inoculum and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010991410.2A CN112126600A (en) | 2020-09-18 | 2020-09-18 | Microbial continuous culture water quality purification microbial inoculum and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112126600A true CN112126600A (en) | 2020-12-25 |
Family
ID=73841158
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010991410.2A Pending CN112126600A (en) | 2020-09-18 | 2020-09-18 | Microbial continuous culture water quality purification microbial inoculum and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112126600A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102649936A (en) * | 2012-05-02 | 2012-08-29 | 武汉合缘绿色生物工程有限公司 | Compound microorganism fungicide for improving water quality of culturing water body and preparation method |
| CN104862244A (en) * | 2015-03-26 | 2015-08-26 | 北京北华中清环境工程技术有限公司 | Efficient composite bacterial agent for removing mixed grease-containing wastewater COD, and applications thereof |
| WO2015184935A1 (en) * | 2014-06-03 | 2015-12-10 | 江南大学 | Efficient bottom treatment bacillus, composite bottom treatment inoculant prepared using same and applications thereof |
-
2020
- 2020-09-18 CN CN202010991410.2A patent/CN112126600A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102649936A (en) * | 2012-05-02 | 2012-08-29 | 武汉合缘绿色生物工程有限公司 | Compound microorganism fungicide for improving water quality of culturing water body and preparation method |
| WO2015184935A1 (en) * | 2014-06-03 | 2015-12-10 | 江南大学 | Efficient bottom treatment bacillus, composite bottom treatment inoculant prepared using same and applications thereof |
| CN104862244A (en) * | 2015-03-26 | 2015-08-26 | 北京北华中清环境工程技术有限公司 | Efficient composite bacterial agent for removing mixed grease-containing wastewater COD, and applications thereof |
Non-Patent Citations (2)
| Title |
|---|
| 王学刚等: "《水处理工程学实验》", 31 October 2016, 北京：冶金工业出版社 * |
| 臧学丽等: "《实用发酵工程技术》", 31 January 2017, 北京：中国医药科技出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100588624C (en) | Microorganism renovation agent of water environment and preparation method thereof | |
| CN107873017B (en) | Microbial compositions and methods for denitrification at high dissolved oxygen levels | |
| CN110655198B (en) | Method for treating nitrogen-containing wastewater by using heterotrophic nitrification-aerobic denitrification paracoccus strain | |
| CN108277174B (en) | Complex microbial inoculant for aquaculture and application thereof | |
| CN109607818A (en) | A kind of microorganism improver of water quality and preparation method thereof | |
| CN108865944B (en) | Microbial live bacteria agent for fish breeding | |
| CN112391308A (en) | Compound microbial agent for treating high-ammonia-nitrogen sewage and preparation method thereof | |
| CN110042074A (en) | A kind of cultural method for straw methane fermentation anaerobic bacteria flora | |
| JP7055304B2 (en) | Lactobacillus parafalaginis strain GBW-HB1903 and its applications | |
| CN111925960B (en) | Halomonas with nitrification and denitrification functions and application thereof | |
| CN112725397A (en) | Method for producing single-cell protein by microbial fermentation | |
| CN109182438B (en) | Production of vitamin B by fermentation of bacillus2Culture medium and culture method | |
| CN109055279B (en) | Composite bacillus preparation and preparation method and application thereof | |
| CN108408921B (en) | Microecological preparation for improving transparency of aquaculture water and preparation method thereof | |
| CN111100830A (en) | Composite microecological preparation for aquaculture water purification and application thereof | |
| CN112126600A (en) | Microbial continuous culture water quality purification microbial inoculum and preparation method thereof | |
| JP2013132248A (en) | Method for culturing photosynthetic bacterium and photosynthetic bacterium | |
| CN101671644A (en) | Method for preparing culture environment purifying agent by spore fermentation waste liquid | |
| CN107937322A (en) | A kind of alkaline medium and its application | |
| CN114107106A (en) | Research and development of compound microbial agent | |
| CN110499265B (en) | Autotrophic nitrifying bacteria consortium and culture expanding method thereof | |
| Alyamani et al. | Effect of denitrifying bacterial biomass and carbon sources on nitrate removal | |
| CN111334449A (en) | Sludge reduction microbial agent, and preparation method and use method thereof | |
| CN112062292B (en) | Black and odorous water body purifying composite microbial agent | |
| CN101054241A (en) | Application of flavobacterium omnivorum in treating sewage at low temperature |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: 325000 3rd floor, building 5, science and technology business incubator, Xinye Road, Binhai New Area, Pingyang County, Wenzhou City, Zhejiang Province Applicant after: Wenzhou Shangda environmental protection Biotechnology Co.,Ltd. Address before: No.238, Youdian Road, Aojiang Town, Pingyang County, Wenzhou City, Zhejiang Province Applicant before: Zhejiang Shangda environmental protection equipment Co.,Ltd. |
|
| CB02 | Change of applicant information | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20201225 |
|
| RJ01 | Rejection of invention patent application after publication |