CN112121108A - Anti-depression essential oil composition with quick response and preparation method thereof - Google Patents
Anti-depression essential oil composition with quick response and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/738—Rosa (rose)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/61—Myrtaceae (Myrtle family), e.g. teatree or eucalyptus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/888—Araceae (Arum family), e.g. caladium, calla lily or skunk cabbage
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/24—Antidepressants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
Abstract
The invention relates to a preparation method of a quick-acting antidepressant essential oil composition, which comprises the following steps: s1 preparing roses; s2 preparing rosemary; s3, preparing clove; s4 preparation of centella asiatica: naturally drying, crushing and sieving for later use, wherein the volume ratio is 10: l, adding entrainer ethanol, stirring uniformly, putting into an extraction kettle, and extracting the centella asiatica by using a supercritical extraction device; s5, preparing acorus gramineus soland; s6, mixing the rose, the rosemary, the clove, the centella asiatica and the grassleaf sweelflag rhizome prepared in S1-S5 according to a certain proportion, and recombining by molecular distillation to prepare the anti-depression essential oil composition.
Description
Technical Field
The invention relates to an essential oil composition, in particular to an anti-depression essential oil composition with quick response and a preparation method thereof.
Background
The existing antidepressant drugs are slow in clinical effect, such as tricyclic drugs (TCAs), Monoamine Transporter Inhibitors (MTIs), monoamine oxidase inhibitors (MAOIs) and the like, and need to be continuously administered for at least 3-6 weeks, so that the treatment compliance of patients is influenced, and the disability and suicide risks of major depressive patients cannot be timely controlled, so that the rapid-effect antidepressant drugs are urgently needed in clinic.
Dujinping et al express four properties from plant volatile oil (essential oil), namely 1, the plant volatile oil can rapidly regulate brain function; 2. the plant volatile oil has obvious antidepressant effect; 3. the chemical components of the plant volatile oil interact with the rapid antidepressant target; 4. the advantage combination improves the clinical onset speed, and combines the physicochemical properties and administration route of the plant volatile oil, the fragrance of the plant volatile oil is considered to be capable of directly stimulating olfactory nerves, the fat solubility is high, the blood brain barrier is easy to permeate, the volatility is strong, the nasal cavity brain targeting and inhalation administration are suitable, the brain function can be rapidly regulated, and the plant volatile oil has the potential of rapid onset and depression resistance.
The plant essential oil has various extraction methods, and each method has advantages, disadvantages and application range, but compared with the traditional methods such as steam distillation, the carbon dioxide supercritical extraction (SFE) method has the advantages of thermal stability, no solvent residue, high efficiency, less energy consumption and the like, and the obtained product has high purity, good activity, safety, less impurities and easier quality control.
The plant essential oil can be applied in a single formula or a compound formula, and the compound formula is mostly applied. For example, in cosmetics, some base oil is often used to increase the stability of crude oil, and it is generally considered that the physiological activity of the essential oil combination is better than that of the single component.
However, the essential oil components are complex, different varieties of essential oils are mixed and compounded, the quality of the obtained mixture is difficult to control due to the fact that chemical components are too complex besides the compatibility of physical properties, and the compound essential oil is not prepared through recombination through molecular distillation at present.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide an anti-depression essential oil composition with quick response and a preparation method thereof.
In order to achieve the purpose, the invention adopts the technical scheme that: a preparation method of a quick-acting antidepressant essential oil composition comprises the following steps:
s1 preparing rose: naturally drying, pulverizing, sieving, and extracting flos Rosae Rugosae with supercritical extraction device;
s2 preparing rosemary: naturally drying, pulverizing, sieving, and extracting herba Rosmarini officinalis with supercritical extraction device;
s3 preparation of clove: naturally air drying flos Caryophylli bud, pulverizing, sieving, and extracting flos Caryophylli with supercritical extraction device;
s4 preparation of centella asiatica: naturally drying, crushing and sieving for later use, wherein the volume ratio is 10: l, adding entrainer ethanol, stirring uniformly, putting into an extraction kettle, and extracting the centella asiatica by using a supercritical extraction device;
s5 preparation of rhizoma acori graminei: naturally drying, and extracting rhizoma Acori Graminei with supercritical extraction device;
s6 mixing the rose, the rosemary, the clove, the centella asiatica and the grassleaf sweelflag rhizome prepared in the S1-S5 according to a certain proportion, and recombining by adopting molecular distillation to prepare the anti-depression essential oil composition.
Further, the environmental parameters of the supercritical extraction device in the steps S1-S5 are as follows:
food grade CO2The purity is not less than 99.9 percent, and the extraction parameters are as follows: CO22The flow is 0.3-25L/h, the extraction time is 1-8h, the temperature is 30-80 ℃, and the pressure of an extraction kettle is 10-30 MPa.
Further, the parameters in the molecular distillation are as follows: the feeding speed is 1-3 mL/min, the molecular distillation temperature is 30-80 ℃, the film scraping rotation speed is 200-.
An anti-depression essential oil composition with quick response is prepared by mixing rose, rosemary, clove, centella and grassleaf sweelflag rhizome according to a certain proportion and recombining by molecular distillation.
Due to the application of the technical scheme, compared with the prior art, the invention has the following advantages:
according to the anti-depression essential oil composition with rapid effect and the preparation method thereof, components contained in the composition are respectively extracted by a carbon dioxide supercritical method, and are mixed in proportion and then recombined by molecular distillation, so that the obtained product has the advantages of deep appearance color, rich fragrance, low impurity content, easiness in quantification and rapid effect of anti-depression.
Detailed Description
The present invention will be described in further detail with reference to specific examples.
Example one
A preparation method of a quick-acting antidepressant essential oil composition comprises the following steps:
s1 preparing rose: naturally air-dry, smash and sieve reserve, recycle supercritical fluid extraction device and extract the rose, the working parameter when extracting is as follows: food grade CO2The purity is not less than 99.9 percent, and the extraction parameters are as follows: CO22The flow is 10L/h, the extraction time is 2h, the temperature is 30 ℃, and the pressure of the extraction kettle is 30 MPa.
S2 preparing rosemary: naturally drying, pulverizing, sieving, and extracting herba Rosmarini officinalis with supercritical extraction device; the working parameters during extraction are as follows: food grade CO2The purity is not less than 99.9 percent, and the extraction parameters are as follows: CO22The flow is 15L/h, the extraction time is 3h, the temperature is 30 ℃, and the pressure of the extraction kettle is 12 MPa.
S3 preparation of clove: naturally air drying flos Caryophylli bud, pulverizing, sieving, and extracting flos Caryophylli with supercritical extraction device; the working parameters during extraction are as follows: food grade CO2The purity is not less than 99.9 percent, and the extraction parameters are as follows: CO22The flow rate is 12L/h, the extraction time is 4h, the temperature is 38 ℃, and the pressure of the extraction kettle is 16 MPa.
S4 preparation of centella asiatica: naturally drying, crushing and sieving for later use, wherein the volume ratio is 10: l, adding entrainer ethanol, stirring uniformly, putting into an extraction kettle, and extracting the centella asiatica by using a supercritical extraction device; the working parameters during extraction are as follows: food grade CO2The purity is not less than 99.9 percent, and the extraction parameters are as follows: CO22The flow rate is 0.8L/h, the extraction time is 3.5h, the temperature is 50 ℃, and the pressure of the extraction kettle is 20 MPa.
S5 preparation of rhizoma acori graminei: naturally drying, and extracting rhizoma Acori Graminei with supercritical extraction device; the working parameters during extraction are as follows: food grade CO2The purity is not less than 99.9 percent, and the extraction parameters are as follows: CO22The flow is 18L/h, the extraction time is 5h, the temperature is 50 ℃, and the pressure of the extraction kettle is 23 MPa.
S6, mixing the rose, the rosemary, the clove, the centella asiatica and the grassleaf sweelflag rhizome prepared in the S1-S5 according to a certain proportion, and recombining by adopting molecular distillation to prepare the anti-depression essential oil composition; the parameters for the molecular distillation are as follows: the feeding speed is 1 mL/min, the molecular distillation temperature is 30 ℃, the film scraping rotation speed is 200r/min, and the distillation pressure is 30 Pa.
Example two
A preparation method of a quick-acting antidepressant essential oil composition comprises the following steps:
s1 preparing rose: naturally air-dry, smash and sieve reserve, recycle supercritical fluid extraction device and extract the rose, the working parameter when extracting is as follows: food grade CO2, purity no less than 99.9%, extraction parameters: the flow rate of CO2 is 20L/h, the extraction time is 5h, the temperature is 60 ℃, and the pressure of the extraction kettle is 23 MPa.
S2 preparing rosemary: naturally drying, pulverizing, sieving, and extracting herba Rosmarini officinalis with supercritical extraction device; the working parameters during extraction are as follows: food grade CO2, purity no less than 99.9%, extraction parameters: the flow rate of CO2 is 13L/h, the extraction time is 5h, the temperature is 35 ℃, and the pressure of the extraction kettle is 18 MPa.
S3 preparation of clove: naturally air drying flos Caryophylli bud, pulverizing, sieving, and extracting flos Caryophylli with supercritical extraction device; the working parameters during extraction are as follows: food grade CO2, purity no less than 99.9%, extraction parameters: the flow rate of CO2 is 18L/h, the extraction time is 3h, the temperature is 58 ℃, and the pressure of the extraction kettle is 20 MPa.
S4 preparation of centella asiatica: naturally drying, crushing and sieving for later use, wherein the volume ratio is 10: l, adding entrainer ethanol, stirring uniformly, putting into an extraction kettle, and extracting the centella asiatica by using a supercritical extraction device; the working parameters during extraction are as follows: food grade CO2, purity no less than 99.9%, extraction parameters: the flow rate of CO2 is 0.9L/h, the extraction time is 5.5h, the temperature is 55 ℃, and the pressure of the extraction kettle is 23 MPa.
S5 preparation of rhizoma acori graminei: naturally drying, and extracting rhizoma Acori Graminei with supercritical extraction device; the working parameters during extraction are as follows: food grade CO2, purity no less than 99.9%, extraction parameters: the flow rate of CO2 is 21L/h, the extraction time is 6h, the temperature is 70 ℃, and the pressure of the extraction kettle is 30 MPa.
S6, mixing the rose, the rosemary, the clove, the centella asiatica and the grassleaf sweelflag rhizome prepared in the S1-S5 according to a certain proportion, and recombining by adopting molecular distillation to prepare the anti-depression essential oil composition; the parameters for the molecular distillation are as follows: the feeding speed is 1 mL/min, the molecular distillation temperature is 30 ℃, the film scraping rotation speed is 200r/min, and the distillation pressure is 30 Pa
Example three.
S1 preparing rose: naturally air-dry, smash and sieve reserve, recycle supercritical fluid extraction device and extract the rose, the working parameter when extracting is as follows: food grade CO2The purity is not less than 99.9 percent, and the extraction parameters are as follows: CO22The flow is 18L/h, the extraction time is 1.5h, the temperature is 40 ℃, and the pressure of the extraction kettle is 16 MPa.
S2 preparing rosemary: naturally drying, pulverizing, sieving, and extracting herba Rosmarini officinalis with supercritical extraction device; the working parameters during extraction are as follows: food grade CO2The purity is not less than 99.9 percent, and the extraction parameters are as follows: CO22The flow rate is 20L/h, the extraction time is 2h, the temperature is 35 ℃, and the pressure of the extraction kettle is 30 MPa.
S3 preparation of clove: naturally air drying flos Caryophylli bud, pulverizing, sieving, and extracting flos Caryophylli with supercritical extraction device; the working parameters during extraction are as follows: food grade CO2The purity is not less than 99.9 percent, and the extraction parameters are as follows: CO22The flow rate is 2L/h, the extraction time is 2h, the temperature is 70 ℃, and the pressure of the extraction kettle is 10 MPa.
S4 preparation of centella asiatica: naturally drying, crushing and sieving for later use, wherein the volume ratio is 10: l, adding entrainer ethanol, stirring uniformly, putting into an extraction kettle, and extracting the centella asiatica by using a supercritical extraction device; the working parameters during extraction are as follows: foodUsing grade CO2The purity is not less than 99.9 percent, and the extraction parameters are as follows: CO22The flow rate is 0.5L/h, the extraction time is 6h, the temperature is 45 ℃, and the pressure of the extraction kettle is 20 MPa.
S5 preparation of rhizoma acori graminei: naturally drying, and extracting rhizoma Acori Graminei with supercritical extraction device; the working parameters during extraction are as follows: food grade CO2, purity no less than 99.9%, extraction parameters: the flow rate of CO2 is 18L/h, the extraction time is 5h, the temperature is 50 ℃, and the pressure of the extraction kettle is 23 MPa.
S6, mixing the rose, the rosemary, the clove, the centella asiatica and the grassleaf sweelflag rhizome prepared in the S1-S5 according to a certain proportion, and recombining by adopting molecular distillation to prepare the anti-depression essential oil composition; the parameters for the molecular distillation are as follows: the feeding speed is 1 mL/min, the molecular distillation temperature is 45 ℃, the film scraping rotation speed is 240r/min, and the distillation pressure is 40 Pa.
In the above examples, the flow rate of entrainer ethanol is 0.05-0.08L/h.
The physical properties of the anti-depression essential oil composition with quick response prepared by the invention are as follows: the obtained recombinant mixture has dark brown color, thick and opaque appearance, strong aroma characteristic, mellow and good body feeling. The appearance color of the compound mixture before recombination is light brown, semitransparent and slightly irritant.
The fast-acting antidepressant essential oil composition is used for carrying out experiments and effects on animals, a chronic unpredictable mild stress model (CUMS) is established, a CUMS combined isolated culture method is adopted to establish a rat depression model, the antidepressant western medicine fluoxetine is used as a positive medicine control, the recombined mixture is used as an experimental object, and the influence on the behavior of the CUMS combined isolated culture depression model rat is observed and compared.
1) Grouping animals
Selecting 80 adult male SD rats with similar spontaneous activity capability and basic sugar water/pure water consumption test and no significant difference in the open field test, and dividing the rats into two batches, wherein the first 40 rats are used for the open field, sugar water consumption and forced swimming test; the second batch 40 was used for water maze testing only; each batch of rats was divided into a normal control group, a model group, a test group and a fluoxetine positive control group, and 4 groups were provided, wherein each group was l0 rats.
2) Animal modeling
With reference to Willner et al, a depression animal model was established with CUMS in conjunction with orphan feeding, and 10 different stress stimulating factors were administered randomly within 21d, including: cutting off water for 24h, fasting for 24h, clamping tail for 1min, shaking (160 times/min, 5min each time), swimming for 5min with ice water at 4 ℃, keeping the environment at 45 ℃ for 5min, reversing day and night for 24h, electrically shocking soles (the voltage is 35V, stimulating every 50s for 1 time, keeping 10s each time, 30 times), binding for 2h and illuminating at night. Only 1 stimulating factor is given daily and each stimulating factor is used 2-3 times cumulatively, in order to avoid the adaptive response of the rat, each stimulating factor is given randomly, so that the rat cannot predict the stimulation in advance. Isolated culture: the rats in the normal control group are 5 rats/cage, and the rats in the rest groups are 1 rat/cage.
3) Method of administration
Normal control group: without any stress stimulus, normal water intake, food intake were performed daily, and a 0.5% concentration sodium carboxymethylcellulose suspension lml/l00g (10 ml/kg. d) was infused daily for 21 days at the same time period as the drug infusion in the stress group, 1 time a day.
Model control group: receiving a random administration of stress factor stimulation every day, and perfusing a 0.5% concentration sodium carboxymethyl cellulose suspension lml/100g (10 ml/kg. d) 30 minutes before each stress; 1 time daily for 1 day.
Test groups: mixed essential oil 0.2ml/100g (20 mg/ml) which is stimulated by a randomly given stress factor and is infused 30 minutes before each stress; 1 time daily for 21 days.
Positive control group: a random stress factor was applied daily and a suspension of 0.154mg/ml fluoxetine 1m1/100g was administered 30 minutes before each stress, 1 time daily for 21 days.
4) And (3) behavioral testing:
(1) open field test
Open field experiments are respectively carried out on day i before the start of the chronic stress and day 22 after the end of the chronic stress, 3 days before the start of the experiments and in the experimental environment every dayThe rats were stroked for 5 minutes, and during the experiment, each rat was placed at a distance of 44X 47cm3In the center of the open field box, an ENV-520 animal behavior video tracking analysis system is used for tracking the track of the rat, the track is continuously recorded for 3 minutes, then the rat is taken out, the bottom and the four walls of the box are cleaned, residual odor of the rat is removed by alcohol with the concentration of 35%, the experiment period is kept quiet, all groups of rats are alternately tested, the total movement distance of each rat is analyzed after the experiment is completed, and individuals with large spontaneous movement capacity difference are removed during the open field experiment test of the first day of the experiment.
(2) Sugar water consumption test
A sugar water consumption test is respectively carried out 1 day before the start of chronic stress stimulation and 21 days after the start of chronic stress stimulation, namely 22 days, before the start of the test, rats are trained to adaptively drink 1% sucrose water, 2 water bottles are placed in each cage, in the first 24 hours, the two bottles are filled with 1% sucrose water, in the subsequent 24 hours, one bottle is filled with l% sucrose water, and the other bottle is filled with pure water.
After the adaptive training is finished, water is forbidden for 24 hours, then a basic sugar water/pure water consumption test of rats is carried out, and two bottles of water are prepared and quantified for each rat simultaneously during the test; one bottle of 1% sucrose water and the other bottle of pure water, and after 1 hour, the two bottles of water are weighed respectively. The total liquid consumption, sugar water consumption, pure water consumption, percentage of sugar water preference (% sugar water preference = sugar water consumption/total liquid consumption × l 00%) of the rats were calculated.
(3) Forced swimming test
The rat is put into a testing bucket with the diameter of 23cm and the height of 60cm, the water temperature in the bucket is 25 +/-1 ℃, the rat is fasted for 12 hours before the experiment but is not forbidden to be water, the rat is forced to swim for 15min in advance on the day I before the experiment, then the rat is taken out, is returned to the cage after being dried by a hair drier, is forced to swim for 5min again after 24 hours, and the time of the rat in the immobile state within 5min is recorded.
(4) Morris Water maze test
In the Morris water maze test, rats were tested for spatial memory extraction 4 times per day for the first 4 days, and on day 5 for rats.
5) Statistical treatment
The significance difference was set at 0.05 (i.e., P < 0.05) as analyzed using SPSS22.0 statistical software. Each set of data is expressed as Mean. + -. standard error (Mean. + -. SEM), except for the water maze experimental data using two-way analysis of variance (two-way, ANOVA) of repeated measures, and the other experimental data using one-way analysis of variance (ANOVA): if the overall difference exists, further pairwise comparison is needed, Leven inspection is adopted for carrying out homogeneity of variance inspection, a Post Hot method is adopted if the variance is uniform, and a Tamhane's T2 method is adopted if the variance is not uniform.
6) Results
(1) Influence of mixture essential oil after recombination on spontaneous activity of CUMS combined with solitary culture depression model rat
No significant difference was detected in the total spontaneous activity course of the rats in each group in the pre-modeling open field test (p > 0.05). Detecting the total spontaneous activity path of each group of rats after modeling is completed, wherein the total is significantly different (p < 0.05); compared with a normal control group, the total spontaneous activity route of the rats in the model group is obviously shortened, and the statistical significance is achieved (p is less than 0.01); compared with the model group, the total spontaneous activity distance of the rats is obviously increased, the statistical significance is achieved (p is less than 0.05), and the rats have no significant difference compared with a normal control group (p is more than 0.05); there was no significant difference in the total course of spontaneous activity of rats between the fluoxetine group and the experimental group (p > 0.05), as shown in table 1:
(2) the influence of the recombined mixture essential oil on the total liquid consumption, the sugar water consumption, the pure water consumption and the sugar water preference percentage in a sugar water consumption experiment of a CUMS combined solitary maintenance depression model rat.
Before the experiment, the total liquid consumption, the sugar water consumption, the pure water consumption and the sugar water preference percentage of rats in each group have no significant difference. After modeling is completed, rats in each group have no significant difference in total liquid consumption (p is greater than 0.05), rats in each group have significant total difference in sugar water consumption (p is less than 0.05), rats in the model group have significantly reduced sugar water consumption and significant difference (p is less than 0.05) compared with a normal control group, and rats in the test group and the model group have significantly increased sugar water consumption and have statistical significance (p is less than 0.05). Compared rats have no significant difference in sugar water consumption among the normal control group, the fluoxetine group and the test group (p > 0.05); the total difference of pure water consumption of rats in each group is obvious (p <0.01), the pure water consumption of rats in the model group is obviously increased compared with that of a normal control group, the difference is obvious (p < 0.05), the pure water consumption of rats in the test group is not obviously different (p > 0.05) compared with that of the model group, the pure water consumption of rats in the fluoxetine group is obviously reduced (p < 0.05) compared with that of the model group, and the pure water consumption of rats in the test group is obviously increased (p < 0.05) compared with that of the fluoxetine group; the percentage of sugar water preference of rats in the model group is remarkably reduced compared with that in the normal control group (p < 0.05), the percentage of sugar water preference of rats in the test group is remarkably increased compared with that in the model group, the statistical significance is achieved (p < 0.05), the percentage of sugar water preference of rats in the test group is not remarkably different from that in the normal control group (p > 0.05), the percentage of sugar water preference of rats in the test group is remarkably reduced compared with that in the fluoxetine group (p < 0.05), and specific results are shown in tables 2 and 3.
(3) And (3) influence of the recombined mixture essential oil on the forced swimming immobility time of the CUMS combined with the parthenogenetic depression model rat.
After the completion of 21 days of modeling, the total immobility time of forced swim was measured for each group of rats on day 22. The total immobility time of each group of experimental rats in forced swimming is obviously different on the whole and has statistical significance (p is less than 0.01); compared with a normal control group, the model group has obvious difference (140.9 +/-19.01 vs89.8 +/-9.9, p is less than 0.01), and compared with the model group, the test group and the fluoxetine group have obvious difference (77.9 +/-10.04, 80.2 +/-10.15 vs140.9 +/-19.01, p is less than 0.01); and the comparison among the normal control group, the fluoxetine group and the test group has no significant difference (p > 0.05).
(4) And (3) influence of the recombined mixture essential oil on spatial learning and reference memory capacity of the CUMS combined solitary depression model rat.
In the Morris Water Maze Test (MWMT), the escape latency is considered to be the time from the entry of the rat into water to the search to the platform, and the length of the escape latency of the rat during training is changed to reflect the superiority and inferiority of the spatial learning ability and the reference memory ability of the rat. The escape latency of each group of rats in MWM training to find the underwater platform is shortened with the increase of the training days, which shows that the spatial learning ability and the reference memory ability of the rats are obviously enhanced with the increase of the training days and have an additive effect (F (3, 117) =22.139, P <0.01), but no significant interaction exists between different drug interventions and the training days (F (9, 108) =0.225, P > 0.05): the escape latencies for the groups of rats to find the platform in the test experiment were overall not significantly different. (F (3, 36) =2.62, p > 0.05), indicating that spatial learning and reference memory function in model rats is not significantly impaired. The time of the rat staying in the target quadrant evacuated from the underwater platform and the times of platform crossing are detected to evaluate the memory and extraction capability of the rat, for example, the longer the time of the rat staying in the target quadrant or the more times of platform crossing indicate that the memory and extraction capability of the rat is stronger, and the memory is firmer. Comparison of residence times in the target quadrant in the rat MWM test of each group showed no significant difference (F (3, 36) =0.85, p > 0.05): comparison of the number of crossing the platform by the water maze test of each group of rats in the MWM test training of 4 consecutive days also shows no significant difference (F (3, 36) =1.44, p > 0.05), which is specifically shown in Table 4.
From the above analysis it follows that: the recombined mixture essential oil can obviously improve depression symptoms of depression animal models, the curative effect is approximately equivalent to that of fluoxetine, but the effect is quick.
The above is only a specific application example of the present invention, and the protection scope of the present invention is not limited in any way. All the technical solutions formed by equivalent transformation or equivalent replacement fall within the protection scope of the present invention.
Claims (4)
1. A preparation method of an anti-depression essential oil composition with quick response is characterized by comprising the following steps:
s1 preparing rose: naturally drying, pulverizing, sieving, and extracting flos Rosae Rugosae with supercritical extraction device;
s2 preparing rosemary: naturally drying, pulverizing, sieving, and extracting herba Rosmarini officinalis with supercritical extraction device;
s3 preparation of clove: naturally air drying flos Caryophylli bud, pulverizing, sieving, and extracting flos Caryophylli with supercritical extraction device;
s4 preparation of centella asiatica: naturally drying, crushing and sieving for later use, wherein the volume ratio is 10: l, adding entrainer ethanol, stirring uniformly, putting into an extraction kettle, and extracting the centella asiatica by using a supercritical extraction device;
s5 preparation of rhizoma acori graminei: naturally drying, and extracting rhizoma Acori Graminei with supercritical extraction device;
s6 mixing the rose, the rosemary, the clove, the centella asiatica and the grassleaf sweelflag rhizome prepared in the S1-S5 according to a certain proportion, and recombining by adopting molecular distillation to prepare the anti-depression essential oil composition.
2. The method for preparing a rapid-onset antidepressant essential oil composition according to claim 1, characterized in that the environmental parameters at the time of extraction in the supercritical extraction apparatus in S1-S5 are as follows:
food grade CO2The purity is not less than 99.9 percent, and the extraction parameters are as follows: CO22The flow is 0.3-25L/h, the extraction time is 1-8h, the temperature is 30-80 ℃, and the pressure of an extraction kettle is 10-30 MPa.
3. The method for preparing a fast-acting antidepressant essential oil composition according to claim 1, characterized in that the parameters of the molecular distillation are as follows: the feeding speed is 1-3 mL/min, the molecular distillation temperature is 30-80 ℃, the film scraping rotation speed is 200-.
4. An antidepressant essential oil composition with rapid onset of action, characterized in that the antidepressant essential oil composition is prepared by mixing rose, rosemary, clove, centella asiatica and grassleaf sweelflag rhizome prepared according to claim 1 in a certain proportion and recombining them by molecular distillation.
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