CN112120038A - Soluble agent for preventing and treating lygus quadratus and application method - Google Patents

Soluble agent for preventing and treating lygus quadratus and application method Download PDF

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CN112120038A
CN112120038A CN202011054841.2A CN202011054841A CN112120038A CN 112120038 A CN112120038 A CN 112120038A CN 202011054841 A CN202011054841 A CN 202011054841A CN 112120038 A CN112120038 A CN 112120038A
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soluble
soluble solution
solvent
weight
agent
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冷阳
钱虹
王凤
汪青松
赵泽彬
叶彬儒
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Shanghai Heben Pharmaceutical Co ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N51/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds having the sequences of atoms O—N—S, X—O—S, N—N—S, O—N—N or O-halogen, regardless of the number of bonds each atom has and with no atom of these sequences forming part of a heterocyclic ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00—Protecting plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Zoology (AREA)
  • Agronomy & Crop Science (AREA)
  • Toxicology (AREA)
  • Biodiversity & Conservation Biology (AREA)
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  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention relates to a soluble solution for controlling Nepholus platyphylla, and a pesticide application method, wherein the soluble solution comprises thiamethoxam accounting for 2-9% of the total weight and emamectin benzoate accounting for 1-3% of the total weight, and the balance comprises a solvent, the soluble solution can be used as a pesticide for controlling the Nepholus platyphylla, and the pesticide application method is to inject 20-40 ml of the soluble solution into a drill hole at the base of a trunk of the Nepholus platyphylla.

Description

Soluble agent for preventing and treating lygus quadratus and application method
Technical Field
The invention relates to a soluble agent for controlling lygus quadratus and a drug application method.
Background
The lygus lucorum has spread from north america to europe and spread to the world since the 60 th century, becoming a worldwide forest pest which is mainly harmful to sycamore species, usually sap is sucked on the back of the bottom leaves of the sycamore crown, yellow and white spots and leaves are green are caused initially, and in severe cases, the leaves are dried from veins to withered yellow, black and necrotic, thereby causing the trees to fall off in advance, the growth of the trees to be interrupted, and the tree vigor to be weak to death; meanwhile, the carrier carries dangerous germs to cause indirect harm, thereby generating larger indirect potential harm. However, the existing medicament has an unsatisfactory control effect on the chinus kunmingensis.
Disclosure of Invention
The invention mainly aims to provide a soluble solution with good control effect on the lygus hesperus, and an application and a pesticide application method thereof.
In order to achieve the purpose, the first aspect of the invention comprises a soluble solution for controlling the triagus lucorum, which comprises 3-5% of thiamethoxam and 2-9% of emamectin benzoate by weight, and the balance of a solvent, wherein the thiamethoxam: the weight ratio of the emamectin benzoate to the emamectin benzoate is (2-3): 1.
preferably, the balance also comprises one or more of an auxiliary agent and water.
Preferably, the auxiliary agent comprises one or more of a cosolvent, an antioxidant and a surfactant.
Preferably, the cosolvent accounts for 30-40% of the total weight of the soluble agent.
Preferably, the cosolvent is selected from one or more of methanol, butanol, isobutanol and diethylene glycol.
Preferably, the antioxidant accounts for 0.3-0.5% of the total weight of the soluble agent.
Preferably, the antioxidant is selected from one or more of BHA, BHT and wgwin D902.
Preferably, the surfactant accounts for 8-12% of the total weight of the soluble agent.
Preferably, the surfactant is selected from one or more of 601, EL90, 33, DISPERSOGEN LFH, tween 20, tween 80.
Preferably, the water accounts for 15-20% of the total weight of the soluble agent.
Preferably, the solvent accounts for 25-35% of the total weight of the soluble agent.
Preferably, the solvent is:
one or more of alpha 150# solvent oil, methyl oleate, gamma-butyrolactone and ARMID DM-10 are mixed in
b. Acetone, NMP, cyclohexanone.
The second aspect of the invention comprises a method for applying the soluble agent, wherein 20-40 ml of the soluble agent is injected into the base of the sycamore trunk by drilling.
The direction of drilling is 45 under the slant, and the aperture of drilling is 5.5mm, and the hole depth is 5 ~ 7 mm.
The soluble medicament can effectively control the lygus quadratus, can be stably stored and has good control effect.
Detailed Description
The soluble solution for preventing and treating the lygus quadratus comprises the main components of thiamethoxam accounting for 2-9% of the total weight and emamectin benzoate accounting for 1-3% of the total weight, and the balance of a solvent, wherein the thiamethoxam: the weight ratio of the emamectin benzoate to the emamectin benzoate is (2-3): 1. according to the research of the invention, the thiamethoxam and emamectin benzoate in the proportioning range can produce synergistic effect on the control of the lygus lucorum. The specific test is as follows:
tests are carried out aiming at thiamethoxam and emamectin benzoate with different proportions, and the test dosage is designed as follows:
Figure BDA0002707047690000031
test work: chinese parasol tree
Test insects: the three-instar larva of Neprid squarrosus is collected from Shanghai street
The test conditions are as follows:
temperature: 23 ℃ -25 DEG C
Relative humidity: 60 to 70 percent
Illumination: light for 16h and dark for 8 h.
3 test method
3.1 feed treatment
Selecting smooth Chinese parasol leaves, cutting into leaf discs with the diameter of about 3 cm, and soaking in each medicament for 2 minutes. The blank control was treated first and the above procedure was repeated in order of the test concentrations from low to high. And (3) after the soaking treatment of the phoenix tree leaves, putting the processed phoenix tree leaves in a cool place, and airing the liquid medicine for later use.
The treated folium Firmianae is placed in culture box with diameter of 4cm at different concentrations, and the treatment is repeated for 3 times. Each culture box is provided with 1 leaf for feeding the three-instar larvae of the quadratus pterus. Each culture box is inoculated with 10 test insects.
Culturing the treated test insects in the test environment, observing the reaction condition of the test insects on the 2 nd day after inoculation, checking the number of dead insects, and calculating the death rate and LC50And LC90Converting the test concentration into a logarithmic value, converting the death rate into a probability value, and calculating linear regression equations (y ═ a + bx) and LC50Value, LC90Values and 95% confidence intervals. The test insects which can not turn over and can not normally crawl are all regarded as dead.
LC calculation Using DPS (v16.05) statistical software50、LC90Equivalent and 95% confidence limits thereof, the insecticidal activity of the medicament is evaluated.
Mortality calculation method:
mortality rate ═ dead insect count 100/total number of insects tested (1)
And calculating the death rate of each treatment according to a formula according to the investigation result, wherein the unit is percentage (%), and the calculation result retains two digits after the decimal point.
Performing regression analysis according to the log value of each drug concentration and the corresponding mortality probability value, and calculating LC of each drug5095% confidence limit. And calculating the co-toxicity coefficient (CTC) of the mixture according to the Sun Yunpei method. The CTC is more than or equal to 120, and the performance of the compound agent is enhanced; the compound agent with CTC less than or equal to 80 shows antagonism; the 80 < CTC < 120 combination showed additive performance. The specific calculation formula is as follows:
ATI＝S×100/M··············(2)
wherein ATI is the actually measured virulence index of the mixed preparation; S-LC 50 for standard medication in milligrams per liter (mg/L); M-LC 50 for the test mixture in milligrams per liter (mg/L).
TTI＝TIA×PA+TIB×PB··············(3)
TTI-theoretical virulence index of the mixture in the formula; TIA-A agent virulence index; the percentage of the PA-A agent in the mixture is expressed as percentage (%); TIB-B agent virulence index; the percentage of the PB-B medicament in the mixture is given as percentage (%).
CTC＝ATI×100/TTI···············(4)
Wherein CTC-co-toxicity coefficient; ATI-measured toxicity index of mixed agent; TTI-theoretical virulence index of the mixture.
Test results
Virulence determination of single and mixed agents
Figure BDA0002707047690000051
Figure BDA0002707047690000061
LC of thiamethoxam to coriolus versicolor50、LC90The values are 6.8809mg/L and 282.3073mg/L respectively, and the LC of emamectin benzoate to corilagin50、LC90The values are 36.7455mg/L and 1261.519mg/L respectively; thiamethoxam: methylamino abamectin benzoate 1: LC of Dermatopteris diptera50、LC90The values are 10.7532mg/L and 239.7599mg/L respectively; thiamethoxam: methylamino abamectin benzoate 1: LC of Dermatopteris diptera50、LC90The values are 10.3024mg/L and 109.1419mg/L respectively; thiamethoxam: methylamino abamectin benzoate 1: LC of Dermatopteris diptera50、LC90The values are 11.2999mg/L and 151.9111mg/L respectively; thiamethoxam: emamectin benzoate 2: LC of Dermatopteris diptera50、LC90The values are 11.6102mg/L and 57.7523mg/L respectively; thiamethoxam: emamectin benzoate 3: LC of Dermatopteris diptera50、LC90The values were 6.5914mg/L and 193.7214mg/L, respectively.
Evaluation of Combined Effect
Agent A of thiamethoxam
And (3) a medicament B: methylamino abamectin benzoate
Figure BDA0002707047690000071
The tests show that the thiamethoxam and the emamectin benzoate have good insecticidal activity on the coriaria sinica linn. After the two are compounded, the addition effect is between 1:3 and 1:1, and the synergy effect is between 2:1 and 3: 1.
On the basis of the mixture ratio, the rest of the mixture ratio of the soluble agent comprises one or more of an auxiliary agent and water besides the solvent. The auxiliary agent can adopt one or more of cosolvent, antioxidant and surfactant. Thereby improving the performances of the soluble agent such as oxidation resistance, stability, dispersibility and the like.
In some embodiments, the co-solvent comprises 30-40% of the total weight of the solubles. The cosolvent is selected from one or more of methanol, butanol, isobutanol and diethylene glycol.
In some embodiments, the antioxidant comprises 0.3 to 0.5% by weight of the total weight of the solubles. The antioxidant is selected from one or more of BHA, BHT and wgwin D902.
In some embodiments, the surfactant comprises 8-12% of the total weight of the solubles. The surfactant is selected from one or more of 601, EL90, 33, DISPERSON LFH, Tween 20, and Tween 80.
In some embodiments, the water comprises 15-20% of the total weight of the solubles.
In some embodiments, the solvent comprises 25-35% of the total weight of the solubles. The solvent is formed by mixing the following two components:
one or more of alpha 150# solvent oil, methyl oleate, gamma-butyrolactone and ARMID DM-10 are mixed in
b. One or more of acetone, NMP and cyclohexanone.
At the time of application, the above-described soluble solution was injected into the bore hole at the base of the trunk of sycamore. When drilling and injecting, the drilling direction is preferably 45 degrees downwards in an inclined mode, the hole diameter of the drilling hole is 5.5mm, the hole depth is 5-7 mm, and the injected dose is 20-40 ml.
The present invention will be further described with reference to the following examples.
Example 1
The soluble solution comprises 96% thiamethoxam technical, 2.8% emamectin benzoate technical, 15% methyl oleate, 15% acetone, 35% isobutanol, 0.3% wgwin D902, 10% tween 20 and 17.7% pure water, wherein the thiamethoxam technical accounts for 4.2% of the total weight.
Example 2
The soluble solution comprises 96% thiamethoxam technical, 1.3% emamectin benzoate technical, 10% r-butyrolactone, 20% acetone, 35% isobutanol, 0.3% BHA, 10% Tween 20 and 17.7% pure water, wherein the thiamethoxam technical accounts for 4.7% of the total weight.
Example 3
The soluble solution comprises 96% thiamethoxam technical, 2.5% emamectin benzoate technical, 15% DM-10, 15% cyclohexanone, 15% isobutanol, 20% diethylene glycol, 0.5% BHT, 10% Tween 20 and 17.5% pure water, wherein the thiamethoxam technical accounts for 4.5% of the total weight.
Example 4
The soluble solution comprises 96% thiamethoxam technical which accounts for 4.8% of the total weight, 2.2% of 70% emamectin benzoate technical, 15% of DM-10, 15% of NMP, 20% of butanol, 15% of diethylene glycol, 0.4% of wgwinD902, 10% of Tween 80 and 17.6% of pure water.
The above soluble solution (formula 1) of example 1 is injected into different sycamore holes respectively, and the injection dosage can be set to be 20 ml-40 ml. The method comprises the specific steps of punching holes at the base of the trunk (50-60 cm from the ground) at an angle of 45 degrees downwards, wherein the hole diameter is 5.5mm, the hole depth is 6-7cm, and 2-3 holes are drilled on the test plant (each hole is not on a horizontal line, and preferably is crossed). Cutting off the tip of the injection bottle by using pruning shears, inserting the injection bottle into the hole drilled by the trunk, and drilling 3-4 small holes at the bottom of the injection bottle by using a minimum drill bit, wherein the injection bottle is counted by 10 ml. After the medicine is injected, the trunk is sealed by wound healing paste to prevent other plant diseases and insect pests from infecting the trunk.
And detecting the prevention and treatment results, wherein the detection method comprises the step of investigating the population base of the lygus quadratus before and 7, 14, 30 and 60 days after treatment. During investigation, each tree selects 4 directions of east, west, south and north, randomly cuts 1 branch in each direction, and counts the number of nymphs and adults on 5 leaves counted from the branch tip. And (5) counting the quantity of the venenum Serpentis, and calculating the reduction rate and prevention effect of population. The results are shown in the following table.
Example 1 control Effect of a soluble solution on Neprilus platyphylla
Figure BDA0002707047690000091
The control effect of 20ml treatment is 82.66% 7 days after punching and injecting the medicine in the formula 1, and the other treatments are all more than 90%.
The control effect of the treatment of 20ml and 30ml is about 95 percent 14 days after the punching and the medicine injection of the formula 1, and the rest is 100 percent.
The prevention and treatment effects are good 30, 60, 90 and 120 days after the medicine is injected by punching the holes in the formula 1.
The embodiments of the present invention are merely illustrative, and not restrictive, of the scope of the claims, and other substantially equivalent alternatives may occur to those skilled in the art and are within the scope of the present invention.

Claims (14)

1. The soluble solution for controlling the triagus quadratus is characterized by comprising thiamethoxam accounting for 2-9% of the total weight and emamectin benzoate accounting for 1-3% of the total weight, and the balance of the soluble solution comprises a solvent, wherein the thiamethoxam: the weight ratio of the emamectin benzoate to the emamectin benzoate is (2-3): 1.
2. the soluble solution of claim 1, wherein the balance further comprises one or more of an adjuvant, water.
3. A soluble solution according to claim 2 wherein the auxiliary agent comprises one or more of a co-solvent, an antioxidant, a surfactant.
4. A soluble agent according to claim 3, wherein the co-solvent comprises 30-40% by weight of the total soluble agent.
5. A soluble solution according to claim 3 wherein the co-solvent is selected from one or more of methanol, butanol, isobutanol, diethylene glycol.
6. A soluble agent according to claim 3 wherein the antioxidant comprises from 0.3 to 0.5% by weight of the total soluble agent.
7. A soluble solution according to claim 3 wherein the antioxidant is selected from one or more of BHA, BHT, wgwin D902.
8. A soluble solution according to claim 3 wherein the surfactant comprises from 8 to 12% by weight of the total soluble solution.
9. A soluble solution according to claim 3 wherein the surfactant is selected from one or more of 601, EL90, 33, DISPERSOGEN LFH, tween 20, tween 80.
10. A soluble agent according to claim 2 wherein water comprises from 15 to 20% by weight of the total soluble agent.
11. A soluble solution according to claim 2 wherein the solvent comprises 25 to 35% by weight of the total soluble solution.
12. A soluble solution according to claim 1 wherein the solvent is:
one or more of alpha 150# solvent oil, methyl oleate, gamma-butyrolactone and ARMID DM-10 are mixed in
b. Acetone, NMP, cyclohexanone.
13. A method of administering a soluble solution according to any one of claims 1 to 12 wherein 20 to 40ml of said soluble solution is injected into a bore hole in the base of the trunk of sycamore.
14. The method of claim 13, wherein the drilling is performed in a 45 ° down slant direction, the diameter of the drilled hole is 5.5mm, and the depth of the drilled hole is 5 to 7 mm.
CN202011054841.2A 2020-09-28 2020-09-28 Soluble agent for preventing and treating lygus quadratus and application method Pending CN112120038A (en)

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