CN110100831A - A kind of injection and application thereof containing emamectin benzoate - Google Patents
A kind of injection and application thereof containing emamectin benzoate Download PDFInfo
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- CN110100831A CN110100831A CN201910389764.7A CN201910389764A CN110100831A CN 110100831 A CN110100831 A CN 110100831A CN 201910389764 A CN201910389764 A CN 201910389764A CN 110100831 A CN110100831 A CN 110100831A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N51/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds having the sequences of atoms O—N—S, X—O—S, N—N—S, O—N—N or O-halogen, regardless of the number of bonds each atom has and with no atom of these sequences forming part of a heterocyclic ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N53/00—Biocides, pest repellants or attractants, or plant growth regulators containing cyclopropane carboxylic acids or derivatives thereof
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- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
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- Engineering & Computer Science (AREA)
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- Pest Control & Pesticides (AREA)
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Abstract
The invention discloses a kind of injection and application thereof containing emamectin benzoate, the raw material of the injection includes emamectin benzoate, chitosan oligosaccharide, organic solvent, surfactant, and the weight ratio of the emamectin benzoate and the chitosan oligosaccharide is 20:(1-100)；The amount of the emamectin benzoate is the 0.1wt% to 10wt% of the injection；Deacetylation >=50% of the chitosan oligosaccharide；The organic solvent is low-carbon alcohols, and the low-carbon alcohols have 1 to 8 carbon atom chain；The amount of the organic solvent is the 0.1wt% to 95wt% of the injection.Suitable for a variety of insect pests of tree trunk injection prevention and treatment forest, at low cost, preventive effect is high, feature applied widely, it is suitble to prevention and treatment Epidemic Disease of Forest, in particular for the borer pests such as pine tree prevention and treatment Monochamus alternatus, and prevention and treatment Bursaphelenchus xylophilus, thus control and prevention pine nematode.
Description
Technical field
The invention belongs to the injections of technical field of pesticide, are a kind of notes containing emamectin benzoate specifically
Penetrate injection and application thereof.
Background technique
China's pine nematode can be withered in tens days after infecting pine tree, is a kind of destructive forestry disaster.Pine
Nematode is mainly propagated by Monochamus alternatus, and Monochamus alternatus imago emergence peak period is pupated before emergence in the 5-8 month, Monochamus alternatus, is being exhaled
Inhaling road endoparasitism has a large amount of Bursaphelenchus xylophilus, migrated after Monochamus alternatus emergence, spray of search for food, cutting oviposition during, it is loose
Material nematode falls off from the respiratory tract of Monochamus alternatus in cutting, and pierces the xylem of pine tree rapidly.
The larva category borer pest of Monochamus alternatus is food with pine tree xylem organization, is the evil with Bursaphelenchus xylophilus symbiosis
Worm is administered from pine tree outside, is equally also difficult to kill the larva of Monochamus alternatus；Bursaphelenchus xylophilus is propagated by Monochamus alternatus imago, pine
Material eelworm harm position is the xylem of pine tree, and can be moved up and down along xvlem fibres tube bank.Therefore pesticide is sprayed on tree
Outside is difficult to kill the Bursaphelenchus xylophilus in trunk xylem.Bursaphelenchus xylophilus is the primitive organism occurred before several hundred million years, is not evolved
Nervous system out, if any machine phosphorus can not kill Bursaphelenchus xylophilus, antibiotic insecticide such as avermectin can be used to prevent traditional pesticide
Bursaphelenchus xylophilus is controlled, but avermectin does not have uptake and translocation, it is difficult to conduction up and down, methylamino AVM hereinafter in the xylem of pine tree
Rhzomorph has certain uptake and translocation, but poor, in the xylem of the trees such as pine tree, the conductibility up and down of long range compared with
Difference.
By tree trunk injection, medicament is directly entered in tree body, is not contacted with ambient enviroment, it is free from environmental pollution and to people and animals and
Other beneficial organisms damage, and are a kind of ideal nuisanceless pesticide application technologies of Environmental compatibility.
Emamectin benzoate is antibiotic insecticide, and be mainly used for prevention and treatment has in lepidoptera pest and Bursaphelenchus xylophilus etc.
Evil biology, but due to due to solubility very little in water, having in plant and its faint uptake and transmission, and hold
Easily flocculation, to constrain application of such drug in agriculture and forestry control of insect.
Conventional liquid formulation for trunk injection, such as the happy desinsection liquid formulation for trunk injection (ZL99111357.8) of 30% dichlorvos oxygen and patent of invention
(patent No.: ZL200510043092.2) " a kind of injection containing imidacloprid " and patent (CN105746566A), these
There is ideal preventive effect to sucking pest, food leaf class pest after patented product tree trunk injection, particularly with prevention and treatment forest
Trunk borer is significant.This injection has several drawbacks in that, firstly, tree vigo(u)r declines after borer pest pierces in trees body
Weak, the especially larva category borer pest of Monochamus alternatus is food with pine tree xylem organization, and pine nematode infects pine tree
Afterwards, it is weak more to renew gesture, in forestry, the time of punching injection liquid formulation for trunk injection is generally long on trunk, especially exists
On pine tree, after being commonly inserted liquid formulation for trunk injection bottle, subsequent wound is seldom carried out again, pine tree tree vigo(u)r can be also further aggravated in this
It is weak；Secondly as emamectin benzoate is macromolecular compound, be not easy the Uptake and translocation in trunk, also, methylamino Ah
Dimension rhzomorph is easy to assemble and crystallize in pine tree trunk, this has more aggravated interior suction of the emamectin benzoate in pine tree trunk and has passed
It leads；In addition, existing injection or injection drying agent solvent for use polar solvent are stronger, DMF or DMSO is such as used, preparation is in trunk
After being diluted, active constituent is easy to assemble and crystallize, to influence drug effect.To which the prior art is not able to satisfy the skill of this field
Art personnel prevent and treat Monochamus alternatus, to prevent pine nematode, realize the corresponding plants protection purpose of forest.
Summary of the invention
The shortcomings that in order to overcome above-mentioned injection, an aspect of of the present present invention purpose are achieved in that
A kind of injection containing emamectin benzoate, the raw material of the injection include methylamino Avermectin
The weight ratio of element, chitosan oligosaccharide, organic solvent, surfactant, the emamectin benzoate and the chitosan oligosaccharide is 20:(1-
100)；The amount of the emamectin benzoate is the 0.1wt% to 10wt% of the injection；The chitosan oligosaccharide it is deacetylated
Degree >=50%；The organic solvent is low-carbon alcohols, and the low-carbon alcohols have 1 to 8 carbon atom chain；The amount of the organic solvent is
The 0.1wt% to 95wt% of the injection.
Further, the organic solvent is selected from methanol, ethyl alcohol, normal propyl alcohol, isopropanol, n-butanol, isobutanol, amylalcohol, first
One of base amylalcohol, cyclohexanol, 2-Ethylhexyl Alcohol, furfuryl alcohol, tetrahydrofurfuryl alcohol, (R)-4-isopropenyl-1-methyl-1-cyclohexene, enanthol, n-octyl alcohol, isooctanol or
It is several.
Preferably, the surfactant is nonionic surface active agent or nonionic surface active agent and anion
The mixture of type surfactant.
Preferably, the nonionic surface active agent one or more surfactants selected from the group below: alcohol polyoxy second
Alkene ether/polyethenoxy ether (oxyalkylate), alkyl phenol polyoxyethylene ether/polyethenoxy ether, nonionic ester.
Further, the nonionic ester is sorbitan alcohol ester, Sorbitan ester APEO/polyoxy third
In the double methyl esters of alkene ether, aliphatic acid polyethenoxy ether/polyethenoxy ether mono-methyl, aliphatic acid polyethenoxy ether/polyethenoxy ether
It is one or several kinds of.
Further, the alkyl phenol polyoxyethylene ether/polyethenoxy ether is selected from castor oil polyoxyethylene ether/polyoxypropylene
Ether, aliphatic acid polyethenoxy ether/polyethenoxy ether, ethoxylated dodecyl alcohol/polyethenoxy ether, nonylphenol polyoxyethylene ether/
Polyethenoxy ether, octyl phenol polyoxyethylene ether/polyethenoxy ether, one in tridecyl alcohol polyoxyethylene ether/polyethenoxy ether
Kind is several.
The anionic surfactant is selected from N- methyl-N- oleyl sodium taurocholate, N- methyl-N- palmityl ox sulphur
Sour sodium, sodium N methyl N oleyltaurate, dioctyl sodium sulphosuccinate and other sodium alkylsulfosuccinates, laurel
Base sodium sulphate, α-(the p- nonyl phenyl)-ω-hydroxyl poly- (ethylene oxide) with average 8-12 moles of ethylene oxide and have flat
α-(the p- octyl phenyl)-ω-hydroxyl poly- (ethylene oxide) of equal 7-12 moles of ethylene oxide, lauryl sodium sulfate, methylene two
One of naphthalene disulfonate, dibutyl naphthalenesulfonic acid salt are several.
Preferably, the raw material further includes water.
Preferably, the raw material further includes pyrethroid insecticides, nicotinic insecticide, chitinase inhibitors class
One of insecticide is several, and the pyrethroid insecticides include effective cypermethrin, Biphenthrin, bromine cyanogen chrysanthemum
One of ester, ethofenprox, effective cypermethrin, Fenpropathrin are several, and the nicotinic insecticide includes imidacloprid, pyrrole aphid
One of ketone, Acetamiprid, Nitenpyram, thiacloprid, Diacloden, clothianidin, dinotefuran are several, and the chitinase inhibits
Agent insecticides include one of chloromethiuron, d ichlorbenzuron, diflubenzuron, flufenoxuron, flubenzuron, tebufenozide, methoxyfenozide or
It is several.
Another aspect of the present invention purpose is achieved in that
A method of pest is invaded and harassed in prevention, confrontation or control plant, and the method includes to the plant and/or plant
The injection as described in claim 1-4 is applied in object part, by injecting the injection to the plant and/or plant part
Injection is prevented, fought or is controlled the invasion of pest described in plant.
Preferably, the pest invasion is the invasion of one or more pests selected from the group below: pine nut worm (tip phycitid category
(Dioryctria spp.)), pine nut seed worm, aphid, bagworm, autumn web spinner, Japanese beetle, gypsymoth, silk tree ermine moth,
Oak poison moth, Americal rice leaf miner, pine needle armored scale, reddish brown mite, sawfly, tent caterpillar, western dragon spruce budworm, winter moth, buprestid beetle, thoroughly
Wing moth worm, ambrosia beetle, roundheaded borer, scolytid, bark beetle, engraver beetle, mountain pine beetle, Southern Pine beetle, dragon spruce
Beetle, west pine beetle, cynipid, including black rubber goitre, lilac longicorn , Ash clearwing moth (lilac borer (Podosesia
syringae))。
Preferably, Bursaphelenchus xylophilus, borer pest are prevented and treated.
Further, the borer pest is Cerambycidae (Cerambycidae) beetle.
Preferably, Bursaphelenchus xylophilus (Bursaphelenchus xylophilus) is prevented and treated.
Further, the injection is injected into pine tree layer of wood by punching, and dosage is 0.1-2.0g a.i./strain, in advance
Anti-, confrontation or control brill moth Monochamus alternatus, to prevent pine nematode.
Beneficial effects of the present invention:
Injection prepared by the present invention containing emamectin benzoate, it is and existing after being applied on pine tree using Stem injection
There is technology to compare, dissolved using alcohol, and enables active constituent emamectin benzoate in pine after addition chitosan oligosaccharide in the formulation
Trunk internal emulsification or even microemulsified are set, goes to make drug spread to Quan Shuzhong using the transpiration pull of pine tree, pine can be killed
Material nematode, chitosan oligosaccharide has certain bactericidal effect, and has mild growth regulating performance, so that pine tree tree vigo(u)r obtains after note is dry
It maintains or even increasees, to reach more preferable preventive effect, reaches the purpose of prevention, it is easy to use and practical.Using institute of the present invention
In the injection of preparation, antibiotic insecticide emamectin benzoate can be used to kill Bursaphelenchus xylophilus, antibiotics desinsection
The combination of agent emamectin benzoate and other pesticides can be used to kill the larva or adult of Monochamus alternatus.It is added to other classes
It is better with insecticide active substance killing effect, and preventive effect can be played.When application, 1-3 is beaten at the near-earth of trunk
A medicine-pouring hole, injects injection of the present invention, and active constituent therein is carried out laterally by the transmission path of trunk and bast
It spreads and is delivered up, go directly tiny branch, and after the xylem and bast of trunk contain pesticide, Bursaphelenchus xylophilus is killed,
Reach the pine tree for curing infection pine nematode, while brill moth can be killed in the alternatus Larva of xylem.Note of the invention
The stabilization of structure can be kept in sap by penetrating injection effective component, kept the concentration with insecticidal activity for a long time in trunk, made
Obtaining injection of the invention has the longer prevention and treatment phase, even up to more than half a year.By using the mode of trunk injection,
Injection is effectively killed the Monochamus alternatus imago migrated and is pierced inside trunk using present invention injection pesticide produced
Alternatus Larva, kill and parasitize Bursaphelenchus xylophilus in trunk, to save control cost and increase substantially control efficiency.
The pine nematode sick tree cure rate of early stage is up to 80% or more.
Injection prepared by the present invention containing emamectin benzoate, after being applied on pine tree trunk using Stem injection,
With only working to target pest organisms, without containing solvent injection packaging material is not damaged to pine tree Small side effects,
It is free from environmental pollution, natural enemy insect is not injured, has the characteristics that at low cost, preventive effect is high.
Specific embodiment
The present invention is described in further detail with reference to embodiments.Pass through the following implementation for being merely illustrative purpose
Example description embodiments of the present invention.
Active constituent emamectin benzoate, Diacloden, effective cypermethrin are pressed hundred weight of folding and are calculated.
Embodiment 1
The present embodiment provides the composition of the injection containing emamectin benzoate, preparation method and quality determining method.
A kind of injection containing emamectin benzoate, the raw material of the injection include methylamino Avermectin
The weight ratio of element, chitosan oligosaccharide, organic solvent, surfactant, the emamectin benzoate and the chitosan oligosaccharide is 20:(1-
100)；The amount of the emamectin benzoate is the 0.1wt% to 10wt% of the injection；The chitosan oligosaccharide it is deacetylated
Degree >=50%；The organic solvent is low-carbon alcohols, and the low-carbon alcohols have 1 to 8 carbon atom chain；The amount of the organic solvent is
The 0.1wt% to 20wt% of the injection.
The emamectin benzoate that embodiment 1 is selected is the 0.1wt% to 10wt% of the injection, specially first ammonia
The content of base avermectin is 2wt%；
Deacetylation >=50% for the chitosan oligosaccharide that embodiment 1 is selected, the deacetylation for the chitosan oligosaccharide specially selected are
92%；The content of the chitosan oligosaccharide of selection is 2wt%；
The organic solvent that embodiment 1 is selected is low-carbon alcohols, and the low-carbon alcohols have 1 to 8 carbon atom chain；Preferably, institute
It states organic solvent and is selected from methanol, ethyl alcohol, normal propyl alcohol, isopropanol, n-butanol, isobutanol, amylalcohol, methyl anyl alcohol, cyclohexanol, 2- second
One of base hexanol, furfuryl alcohol, tetrahydrofurfuryl alcohol and (R)-4-isopropenyl-1-methyl-1-cyclohexene, enanthol, n-octyl alcohol, isooctanol are several, specially isopropanol
4wt%；
The decentralized medium of embodiment 1 is methanol, supplies component to 100wt% with methanol.
Surfactant described in the surfactant that embodiment 1 is selected is nonionic surface active agent or non-ionic table
The mixture of face activating agent and anionic surfactant, specially nonionic surface active agent and anionic surface are living
Property agent mixture, further, be alcohol polyoxyethylene ether 4wt%, dioctyl sodium sulphosuccinate 3.5wt%.
The preparation method of the injection of the present embodiment, comprising the following steps:
1) raw material preparation step: weighing the recipe ingredient of the injection according to said ratio, matches what is weighed
Fang Zufen is put into beaker；The emamectin benzoate for weighing 20g percentage amount is dissolved with 60g methanol, completely rear addition to be dissolved
40g isopropanol, adds alcohol polyoxyethylene ether 40g, and dioctyl sodium sulphosuccinate 35g obtains emamectin benzoate solution；
Chitosan oligosaccharide, is dissolved to transparent by the chitosan oligosaccharide 20g for weighing selection with 200g methanol, obtains chitosan oligosaccharide solution；
2) it is uniformly mixed step: remaining methanol is added in above-mentioned emamectin benzoate solution, it is molten to add chitosan oligosaccharide
Liquid stirs evenly, and obtains the injection 1000g of the present embodiment.
The injection 5mL being prepared is taken, 95mL water is separately added into and is mixed, observes its stabilization after standing 48 hours
Property, then 5mL is taken to be added in vial, 14 days are stood for 52 ± 2 DEG C in baking oven, observe its stability；Transparent polyester is added in 50mL
In bottle, 14,28,56 days are stood for 0 ± 2 DEG C in refrigerator, observes its stability, if has crystallization to be precipitated.
The results show that embodiment 1 prepare 2% emamectin benzoate injection can stable dispersion in aqueous solution,
And there is good hot and cold storage stability.
Embodiment 2
Embodiment 2 the difference from embodiment 1 is that, the effective component of the recipe ingredient of the injection of embodiment 2 is also
One of pyrethroid insecticides, nicotinic insecticide, chitinase inhibitors insecticides are several, specially quasi-
Pyrethrins insecticide, it is therefore intended that, increase the insecticidal spectrum of injection, and improve medicament in the drug effect of pine tree root.
The present embodiment provides the composition of the injection containing emamectin benzoate, preparation method and quality determining method.
A kind of injection containing emamectin benzoate preventing pine nematode, the raw material packet of the injection
Include emamectin benzoate, chitosan oligosaccharide, organic solvent, surfactant, the emamectin benzoate and the chitosan oligosaccharide
Weight ratio is 20:(1-100)；The amount of the emamectin benzoate is the 0.1wt% to 10wt% of the injection；It is described
Deacetylation >=50% of chitosan oligosaccharide；The organic solvent is low-carbon alcohols, and the low-carbon alcohols have 1 to 8 carbon atom chain；It is described
The amount of organic solvent is the 0.1wt% to 95wt% of the injection；The raw material further include pyrethroid insecticides,
One of nicotinic insecticide, chitinase inhibitors insecticides are several, and the pyrethroid insecticides include
One of effective cypermethrin, Biphenthrin, decis, ethofenprox, effective cypermethrin, Fenpropathrin are several, institute
State nicotinic insecticide include imidacloprid, pymetrozine, Acetamiprid, Nitenpyram, thiacloprid, Diacloden, clothianidin, in dinotefuran
One or several kinds, the chitinase inhibitors insecticides include chloromethiuron, d ichlorbenzuron, diflubenzuron, flufenoxuron, fluorine bell
One of urea, tebufenozide, methoxyfenozide are several.
The emamectin benzoate that embodiment 2 is selected is the 0.1wt% to 10wt% of the injection, specially first ammonia
The content of base avermectin is 1wt%；
The raw material that embodiment 2 is selected further includes pyrethroid insecticides, nicotinic insecticide, chitinase inhibitors
One of insecticides are several, specially pyrethroid insecticides, are further effective cypermethrin, content is
4wt%；
Deacetylation >=50% for the chitosan oligosaccharide that embodiment 2 is selected, the deacetylation for the chitosan oligosaccharide specially selected are
85%；The content of the chitosan oligosaccharide of selection is 1.5wt%；
The organic solvent that embodiment 2 is selected is low-carbon alcohols, and the low-carbon alcohols have 1 to 8 carbon atom chain；Preferably, institute
It states organic solvent and is selected from methanol, ethyl alcohol, normal propyl alcohol, isopropanol, n-butanol, isobutanol, amylalcohol, methyl anyl alcohol, cyclohexanol, 2- second
One of base hexanol, furfuryl alcohol, tetrahydrofurfuryl alcohol and (R)-4-isopropenyl-1-methyl-1-cyclohexene, enanthol, n-octyl alcohol, isooctanol are several, specially isopropanol
4wt%, n-octyl alcohol 2wt%, tetrahydrofurfuryl alcohol wt%；
Since the decentralized medium of embodiment 2 is water, component is supplied to 100wt% with water.
Surfactant described in the surfactant that embodiment 2 is selected is nonionic surface active agent or non-ionic table
The mixture of face activating agent and anionic surfactant, specially nonionic surface active agent and anionic surface are living
Property agent mixture, further, be nonylphenol polyoxyethylene ether 3.5wt%, N- methyl-N- oleyl sodium taurocholate 2wt%.
The preparation method of the injection of the present embodiment, comprising the following steps:
1) raw material preparation step: weighing the recipe ingredient of the injection according to said ratio, matches what is weighed
Fang Zufen is put into beaker；The emamectin benzoate for weighing 10g percentage amount is dissolved with 20g isopropanol and 20g n-octyl alcohol, to
Nonylphenol polyoxyethylene ether 35g is added after dissolution completely, N- methyl-N- oleyl sodium taurocholate 20g obtains methylamino Ah
Tie up rhzomorph solution；The effective cypermethrin for weighing the 40g percentage amount of selection, has been dissolved with 20g isopropanol and 40g tetrahydrofurfuryl alcohol
Entirely, effective cypermethrin solution is obtained；After mixing by effective cypermethrin solution and emamectin benzoate solution, it obtains
Oily phase；Chitosan oligosaccharide, is dissolved to transparent by the chitosan oligosaccharide 15g for weighing selection with water, obtains water phase；
2) it is uniformly mixed step: above-mentioned oil being added to water phase, high-speed stirred is uniform, obtains the injection needle of the present embodiment
Agent 1000g.
The injection 5mL being prepared is taken, 95mL water is separately added into and is mixed, observes its stabilization after standing 48 hours
Property, then 5mL is taken to be added in vial, 14 days are stood for 52 ± 2 DEG C in baking oven, observe its stability；Transparent polyester is added in 50mL
In bottle, 14,28,56 days are stood for 0 ± 2 DEG C in refrigerator, observes its stability, if has crystallization to be precipitated.
The results show that 5% emamectin benzoate effective cypermethrin injection prepared by embodiment 2 can be stablized point
It dissipates in aqueous solution, and there is good hot and cold storage stability.
Embodiment 3
Embodiment 3 the difference from embodiment 1 is that, the effective component of the recipe ingredient of the injection of embodiment 3 is also
One of pyrethroid insecticides, nicotinic insecticide, chitinase inhibitors insecticides are several, specially cigarette
Bases insecticide, it is therefore intended that, increase the insecticidal spectrum of injection, and improve medicament in the drug effect of pine tree bizet.
The present embodiment provides the composition of the injection containing emamectin benzoate, preparation method and quality determining method.
A kind of injection containing emamectin benzoate preventing pine nematode, the raw material packet of the injection
Include emamectin benzoate, chitosan oligosaccharide, organic solvent, surfactant, the emamectin benzoate and the chitosan oligosaccharide
Weight ratio is 20:(1-100)；The amount of the emamectin benzoate is the 0.1wt% to 10wt% of the injection；It is described
Deacetylation >=50% of chitosan oligosaccharide；The organic solvent is low-carbon alcohols, and the low-carbon alcohols have 1 to 8 carbon atom chain；It is described
The amount of organic solvent is the 0.1wt% to 95wt% of the injection；The raw material further include pyrethroid insecticides,
One of nicotinic insecticide, chitinase inhibitors insecticides are several, and the pyrethroid insecticides include
One of effective cypermethrin, Biphenthrin, decis, ethofenprox, effective cypermethrin, Fenpropathrin are several, institute
State nicotinic insecticide include imidacloprid, pymetrozine, Acetamiprid, Nitenpyram, thiacloprid, Diacloden, clothianidin, in dinotefuran
One or several kinds, the chitinase inhibitors insecticides include chloromethiuron, d ichlorbenzuron, diflubenzuron, flufenoxuron, fluorine bell
One of urea, tebufenozide, methoxyfenozide are several.
The emamectin benzoate that embodiment 3 is selected is the 0.1wt% to 10wt% of the injection, specially first ammonia
The content of base avermectin is 0.5wt%；
The raw material that embodiment 3 is selected further includes pyrethroid insecticides, nicotinic insecticide, chitinase inhibitors
One of insecticides are several, specially nicotinic insecticide, are further Diacloden, content 3wt%；
Deacetylation >=50% for the chitosan oligosaccharide that embodiment 3 is selected, the deacetylation for the chitosan oligosaccharide specially selected are
50%；The content of the chitosan oligosaccharide of selection is 2.5wt%；
The organic solvent that embodiment 3 is selected is low-carbon alcohols, and the low-carbon alcohols have 1 to 8 carbon atom chain；Preferably, institute
It states organic solvent and is selected from methanol, ethyl alcohol, normal propyl alcohol, isopropanol, n-butanol, isobutanol, amylalcohol, methyl anyl alcohol, cyclohexanol, 2- second
One of base hexanol, furfuryl alcohol, tetrahydrofurfuryl alcohol and (R)-4-isopropenyl-1-methyl-1-cyclohexene, enanthol, n-octyl alcohol, isooctanol are several, specially isopropanol
4wt%, methanol 8wt%, n-octyl alcohol 1wt%；
It is water since embodiment 3 is decentralized medium, component is supplied to 100wt% with water.
Surfactant described in the surfactant that embodiment 3 is selected is nonionic surface active agent or non-ionic table
The mixture of face activating agent and anionic surfactant, specially nonionic surface active agent and anionic surface are living
Property agent mixture, further, be phenethyl phenol polyethenoxy ether 9wt%, dioctyl sodium sulphosuccinate 8wt%.
The preparation method of the injection of the present embodiment, comprising the following steps:
1) raw material preparation step: weighing the recipe ingredient of the injection according to said ratio, matches what is weighed
Fang Zufen is put into beaker；The emamectin benzoate for weighing 5g percentage amount is dissolved with 10g methanol, completely rear addition to be dissolved
Phenethyl phenol polyethenoxy ether 90g is added, dioctyl sodium sulphosuccinate 80g obtains emamectin benzoate solution；Weigh choosing
The thiacloprid of 30g percentage amount is dissolved completely with 40g isopropanol, 70g methanol and 10g n-octyl alcohol, obtains Diacloden solution；
After mixing by Diacloden solution and emamectin benzoate solution, oily phase is obtained；The chitosan oligosaccharide 25g for weighing selection, uses water
Chitosan oligosaccharide is dissolved to transparent, obtains water phase；
2) it is uniformly mixed step: above-mentioned oil being added to water phase, after mixing evenly, obtains the injection of the present embodiment
1000g。
The injection 5mL being prepared is taken, 95mL water is separately added into and is mixed, observes its stabilization after standing 48 hours
Property, then 5mL is taken to be added in vial, 14 days are stood for 52 ± 2 DEG C in baking oven, observe its stability；Transparent polyester is added in 50mL
In bottle, 14,28,56 days are stood for 0 ± 2 DEG C in refrigerator, observes its stability, if has crystallization to be precipitated.
The results show that 3.5% emamectin benzoate Diacloden injection prepared by embodiment 3 can be stably dispersed in
In aqueous solution, and there is good hot and cold storage stability.
Comparative example
Emamectin benzoate 2g, 15g DMF, 10g polysorbate60 are weighed, methanol complements to 100g.First is dissolved with 15gDMF
Amino avermectin 1.8g after stirring is dissolved completely, is mixed, is added 10g surface active agent tween 60, be sufficiently stirred molten
Solution is added methanol and is supplemented to 100g to get 1.8% avermectin injection.
Comparative experimental example
Field controling test
In order to verify the control efficiency of the injection Bursaphelenchus xylophilus disease prepared by the present invention containing emamectin benzoate,
By the injection made from embodiment 1,2,3 containing emamectin benzoate and using made from conventional needle agent compounding method
Comparative example medicament has carried out the pharmacodynamic test of woodland note dry application medicine prevention and treatment trees insect pest and disease, and specific test result is as follows.
One, the test of injection prevention and treatment pine nematode
1 materials and methods
1.1 for reagent tool
1.1.1 medicament
3 active constituent injections (self-control)；
Embodiment 1-3 and comparative example gained medicament
1.1.2 medical instruments
Portable rechargeable-type electric hand drill, injection syringe etc..
1.2 for examination tree species and controlling object
1.2.1 for trying tree species
Test site is four ridge reservoir area of the Hangzhou Yuhang District town Jing Shan, and sample plot is the raw masson pine (Pinus of 8-15
Massoniana) with the wealthy Secondary Mixed Forest of pine, pine nematode is high-incidence for years in this place.On 2 6th, 2016 injection masson pines
120 plants of meter.It infects under natural conditions, not artificial infection cause of disease, on March 7th, 2016,10 daily inspection of April 6, May 6 and August
Incidence, and drill at high 7m setting, the Pine plants tissue of drilling is taken out, to detect the concentration of the agent activity ingredient,
Using experimental field 240 plants of masson pines of other mark as control.
1.2.2 controlling object
Monochamus alternatus, Bursaphelenchus xylophilus
1.3 test method
Four ridge reservoir area pine forests of the Yuhang District town Jing Shan of 2 months Hangzhous on the 6th in 2016 are tested, to pine tree experimental field
Standing forest region is tested, and different zones keep certain distance to carry out geographic isolation, to guarantee that pest control experiment is not concerned with mutually
It disturbs.Medicament, comparative example medicament, the CK (clear water control) of different zones application embodiment 2-6 is prevented and treated, and has pine with last year
Material nematodiasis region is Experimental Area, not there is obvious morbidity pine tree for experiment pine tree, measures pine tree diameter, experiment pine tree is chosen
Diameter 25-30cm is that a processing group carries out medicament experiment, three repetitions of each processing with 10 pine trees.Around injection pine tree
It is reference examples (CK) that region, which chooses 20 not having obvious morbidity pine tree,.Method of administration is using trunk punching note common in forestry
Medicine, the drug concentration of every pine tree injection of all trial zones are consistent (with active ingredient), are carried out on 2 6th, 2016
Tree trunk injection, the base portion with electric drill in tree tilt down 15 degree of punchings apart from ground 20cm or so place, and aperture 0.4cm, hole depth is about
3-4cm.Self-flowing medicine injection device tip equipped with medicament is cut out, inserts in the hole after being broken blind mouth with needle, infuses injection
Medicament is injected tree body by emitter.Medicament is injected by the amount that every tree emamectin benzoate active constituent injection amount is 1.0g, in pine
It sets and beats 1-3 deep 1 centimetre or so of medicine-pouring hole at the close ground of trunk, institute of the embodiment of the present invention is injected in each medicine-pouring hole
Then the pesticide of production uses red paint marker number on trunk.
1.4 drug effect inspections
30th day, 60 days, 90 days after injection medicament drill at high 7m setting, take out the Pine plants tissue of drilling, with inspection
The concentration for surveying the agent activity ingredient, the results are shown in Table 1.It injects after medicament and counts the pine nematode incidence of pine tree after 2 years,
It the results are shown in Table 2.It investigates the worm channel number that each processing pine tree has fresh defecation before injection, is Monochamus alternatus insect population, i.e., it is living before medicine
Borer population, injection are investigated after 5,10,15 days there are also fresh defecation worm channel number on each processing pine tree, i.e., borer population living after medicine, after 40 days again
Secondary investigation the results are shown in Table 3 to record long control effect.
Monochamus alternatus preventive effect calculation formula is as follows:
Borer population × 100 living before Revision insect recluced rate (%)=(borer population living after borer population-medicine of living before medicine)/medicine
Preventive effect (%)=(treatment region Revision insect recluced rate-check plot Revision insect recluced rate)/(check plot 100- Revision insect recluced rate)
×100
1.5 results and analysis
Transmission dynamic of 1 medicament of table in horse hair pine trunk
As shown in Table 1, pipette will be compareed significantly larger than in the medicament of the present invention emamectin benzoate in pine tree trunk
Example medicament, reason is that chitosan oligosaccharide has certain bactericidal effect, and has mild growth regulating performance, so that pine tree after note is dry
Tree vigo(u)r is maintained and even improves, and have it is certain help uptake and translocation effect, to increase emamectin benzoate in pine tree tree
Do interior conduction.Same dose emamectin benzoate (embodiment 1-3 and reference examples) are injected in trunk, the embodiment of the present invention
Interior pipette 30d, 60d, the 90d of 1-3 sample in trunk are intended to significantly larger than reference examples, and reference examples are due to emamectin benzoate
Substantially uptake and translocation is poor in pine tree body, so 30d is not detected substantially, the concentration that 60d-90d is detected also below
10 μ g/g, and it is far below interior pipette of the embodiment 1-3 sample in trunk.Embodiment 1 to embodiment 3 the experimental results showed that,
Until the interior position for being drawn to 7m, the ratio of active constituent is generally consistent in 60d-90d, this is also last active constituent hair
It shoots due drug effect and guarantee is provided.
2 medicament of table prevents and treats experiment to the forest farm of Bursaphelenchus xylophilus
| Reagent agent | Dosage (g a.i. /) | Disease incidence |
| Embodiment 1 | 1.0 | 13.3% |
| Embodiment 2 | 1.0 | 10.0% |
| Embodiment 3 | 1.0 | 10.0% |
| Reference examples | 1.0 | 26.7% |
| It compares (CK) | - | 43.3% |
As seen from Table 1, untreated pine tree (CK), pine nematode incidence is apparently higher than that treated pine tree；It adopts
It is lower than existing control agent with the disease incidence of insecticide of the present invention (embodiment 1 to embodiment 3) treated pine tree, pine nematode
(comparative example) shows that the insecticide of present invention prevention and treatment Monochamus alternatus Hope can preferably prevent and treat Monochamus alternatus Hope, and then has reached inhibition pine
The purpose that material nematodiasis is propagated；And insecticide of the present invention has formula simply, easy to use, the feature of lasting period length.
Control efficiency of several medicaments of table 3 to Monochamus alternatus
As shown in Table 3, injection medicament prepared by the present invention has and the comparable medicine of reference examples on prevention and treatment Monochamus alternatus
Effect, 40 days after injection, does not find fresh frass again.But injection prepared by the present invention has better quick-acting,
Embodiment 1, embodiment 2, embodiment 3 did not find fresh frass after 10 days again, showed to kill prevention and treatment region completely
Alternatus Larva on interior pine tree, embodiment 2 even just do not find fresh frass again after 5 days.This shows relative to control
The injection of example, preparation of the embodiment of the present invention has more preferably uptake and translocation, can faster conduct medicament to target position
It sets, plays drug effect.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, is all covered by the present invention.
Claims (10)
1. a kind of injection containing emamectin benzoate, which is characterized in that the raw material of the injection includes first ammonia
Base avermectin, chitosan oligosaccharide, organic solvent, surfactant, the weight ratio of the emamectin benzoate and the chitosan oligosaccharide
For 20:(1-20)；The amount of the emamectin benzoate is the 0.1wt% to 10wt% of the injection；The chitosan oligosaccharide
Deacetylation >=50%；The organic solvent is low-carbon alcohols, and the low-carbon alcohols have 1 to 8 carbon atom chain, described organic molten
The amount of agent is the 1wt% to 95wt% of the injection.
2. injection according to claim 1, which is characterized in that the surfactant is living selected from non-ionic surface
Property agent, anionic surfactant and its mixture.
3. injection according to claim 1, which is characterized in that the raw material further includes water.
4. injection according to claim 1, which is characterized in that the raw material further includes pyrethroid desinsection
One of agent, nicotinic insecticide, chitinase inhibitors insecticides are several, the pyrethroid insecticides packet
One of effective cypermethrin, Biphenthrin, decis, ethofenprox, effective cypermethrin, first cyanogen chrysanthemum rouge or several are included,
The nicotinic insecticide includes imidacloprid, pymetrozine, Acetamiprid, Nitenpyram, thiacloprid, Diacloden, clothianidin, dinotefuran
One of or it is several, the chitinase inhibitors insecticides include chloromethiuron, d ichlorbenzuron, diflubenzuron, flufenoxuron, fluorine bell
One of urea, tebufenozide, methoxyfenozide are several.
5. a kind of method that pest is invaded and harassed in prevention, confrontation or control plant, the method includes to the plant and/or plant
Injection of the part application as described in claim 1-4, by injecting the injection needle to the plant and/or plant part
Agent is prevented, fought or is controlled the invasion of pest described in plant.
6. according to the method described in claim 5, it is characterized in that, it is one or more evils selected from the group below that the pest, which is invaded and harassed,
The invasion of worm: pine nut worm (tip phycitid category (Dioryctria spp.)), pine nut seed worm, aphid, bagworm, autumn web spinner,
Japanese beetle, gypsymoth, silk tree ermine moth, oak poison moth, Americal rice leaf miner, pine needle armored scale, reddish brown mite, sawfly, tent caterpillar, west
Portion dragon spruce budworm, winter moth, buprestid beetle, clearwing moth worm, ambrosia beetle, roundheaded borer, scolytid, bark beetle, engraver beetle,
Mountain pine beetle, Southern Pine beetle, dragon spruce beetle, west pine beetle, cynipid, including black rubber goitre, lilac longicorn , Ash clearwing moth
(lilac borer (Podosesia syringae)).
7. according to the method described in claim 5, it is characterized in that, prevention and treatment Bursaphelenchus xylophilus, borer pest.
8. the method according to the description of claim 7 is characterized in that the borer pest is Cerambycidae (Cerambycidae)
Beetle.
9. according to the method described in claim 5, it is characterized in that, prevention and treatment Bursaphelenchus xylophilus (Bursaphelenchus
xylophilus)。
10. method according to claim 8 or claim 9, which is characterized in that the injection is injected into pine tree wood by punching
Matter layer, dosage are 0.1-2.0g a.i./strain, prevention, confrontation or control brill moth Monochamus alternatus, to prevent pine nematode.
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| CN111713502A (en) * | 2020-06-09 | 2020-09-29 | 郑州昌安植物保护有限公司 | Preparation for preventing and treating longicorn and preparation method thereof |
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| CN113115783A (en) * | 2021-04-12 | 2021-07-16 | 南京生兴有害生物防治技术股份有限公司 | Preparation method of curing agent for pine wood nematode disease |
| CN113349216A (en) * | 2021-05-24 | 2021-09-07 | 驭农科技(浙江)有限公司 | Medicament for preventing and treating pine wood nematode disease and use method thereof |
| CN113475528A (en) * | 2021-07-02 | 2021-10-08 | 山东省林业科学研究院 | Quick healing agent for drug injection holes of pine trees |
| CN114467980A (en) * | 2022-03-28 | 2022-05-13 | 广西壮族自治区国有七坡林场 | Monochamus alternatus trapping agent and preparation method thereof |
| CN114467980B (en) * | 2022-03-28 | 2023-11-03 | 广西壮族自治区国有七坡林场 | Monochamus alternatus trapping agent and preparation method thereof |
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Application publication date: 20190809 |