CN109430258A - A kind of trunk injection liquor for the containing graphene nano material preventing and treating diaphorina citri - Google Patents
A kind of trunk injection liquor for the containing graphene nano material preventing and treating diaphorina citri Download PDFInfo
- Publication number
- CN109430258A CN109430258A CN201811644558.8A CN201811644558A CN109430258A CN 109430258 A CN109430258 A CN 109430258A CN 201811644558 A CN201811644558 A CN 201811644558A CN 109430258 A CN109430258 A CN 109430258A
- Authority
- CN
- China
- Prior art keywords
- trunk injection
- liquor
- graphene
- injection liquor
- active constituent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/707—1,2,3- or 1,2,4-triazines; Hydrogenated 1,2,3- or 1,2,4-triazines
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
- A01N47/44—Guanidine; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N51/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds having the sequences of atoms O—N—S, X—O—S, N—N—S, O—N—N or O-halogen, regardless of the number of bonds each atom has and with no atom of these sequences forming part of a heterocyclic ring
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of trunk injection liquor of containing graphene nano material for preventing and treating diaphorina citri, the raw materials including following mass percent: active constituent: 0.2-20.0, graphene nano material: 0.1-30；Weight ratio 100:10~100:500 of the active constituent and the graphene nano material, the pesticide activity component includes nicotinic insecticide.Trunk injection liquor prepared by the present invention is used for tree trunk injection, after injecting citrus layer of wood, active constituent has well conductibility up and down in citrus layer of wood, conduction is also easy in terms of horizontal conductive to citrus bast, so as to be transmitted to tender tip and root, thus achieve the effect that prevent and treat diaphorina citri, the trunk injection liquor that the present invention is prepared is for preventing and treating diaphorina citri, correcting controling effect can reach 93.4% after 40 days, to achieve the purpose that prevent Citrus Huanglongbing pathogen.
Description
Technical field
The invention belongs to the trunk injection agent of technical field of plant protection, are a kind of prevention and treatment diaphorina citris specifically
The trunk injection liquor of containing graphene nano material.
Background technique
The adult and nymph of diaphorina citri directly suck the phloem sap of the bud tip, not only influence the normal life of citrus plant
It is long, and Citrus Huanglongbing pathogen is propagated during feeding.Susceptible tree tree vigo(u)r decline, gradually loses bearing capacity, final tree body is dead
It dies.Diaphorina citri and yellow twig become a great problem for restricting Citrus Industry sustainable development all over the world.Chemical prevention at present
Diaphorina citri is to administer the important measures of Citrus Huanglongbing pathogen.
The generation of diaphorina citri and mandarin tree pumping hair young sprout are closely related, and Citrus leaf is more dense, to accomplish full side
Position, efficiently, prevent and treat diaphorina citri for a long time, can just make mandarin tree from its evil.Spray pesticide prevent and treat diaphorina citri exist with
Lower problem: medicament sprinkling is uneven, and the wood louse in shelter is difficult to prevent and treat；Spraying time and spraying times are difficult to control；Medication
Spend frequent and ineffective, drug resistance and environmental pollution is serious.
By tree trunk injection, medicament is directly entered in tree body, is not contacted with ambient enviroment, it is free from environmental pollution and to people and animals and
Other beneficial organisms damage, and are a kind of ideal nuisanceless pesticide application technologies of Environmental compatibility.Pesticide efficacy
Height, lasting period are long；It can replace foliage application or reduce blade face dosage；Reduce Pesticide use input cost (cost of labor, combustion
Oil takes)；Mitigate machinery to soil packing and to the damage of crop；Reduce the influence etc. to non-target organism.
There is the pesticide activity component kind of good absorbability few, even the pesticide activity component product with absorbability
Kind, such as anabasine insecticide, its mechanism of action are that ((nAChR) agonist, there is one to nicotinic acetylcholine alkali enzyme acceptor
Fixed absorbability is easy to be absorbed by crop roots such as crop such as wheats, transporting to the tender position of children on the ground, but is not easy by woody plant
Object plant is conducted to its tree crown portion, or is conducted after being absorbed by tree crown tender tip or blade to root.Such as citrus, especially
3 years or more raw mandarin trees, tree is generally up to 2-5 meters high, and root system is difficult conduction to tree crown, Huo Zhechuan after absorbing pesticide activity component
After leading tree crown, since concentration is inadequate, it is not enough to prevent and treat diaphorina citri.Traditional method is the operation simultaneously of pouring root and foliar spray,
Even only foliar spray operation.But after citrus infection yellow twig, root is impaired, be unable to good absorption pesticide activity at
Point, and foliar spray, due to being lost, the factors such as evaporation, Foliage Absorption pesticide activity component is also limited, also easily causes environment dirty
Dye.
Conventional liquid formulation for trunk injection, such as the happy desinsection liquid formulation for trunk injection (ZL99111357.8) of 30% dichlorvos oxygen and patent of invention
(patent No.: ZL200510043092.2) " a kind of trunk injection agent of the beautiful jade of worm containing pyrrole " and patent (CN105746566A), this
There is ideal preventive effect to sucking pest, food leaf class pest after a little patented product tree trunk injections, particularly with prevention and treatment woods
The wooden trunk borer is significant, but preparation system is mainly missible oil system, in a solvent for pesticide activity component dissolution, is added
Surfactant.This trunk injection agent has several drawbacks in that, firstly, since it is missible oil system, in the formulation pesticide activity at
It is divided into molecular state, but after it is injected into trees body, is emulsified into particle, grain diameter is at 0.5-2 μm, even more greatly,
And can constantly grow up with the time, this directly affects pesticide activity component and passes in the intracorporal transport of plant；Secondly because needing
Pesticide activity component is dissolved with solvent, it is on the one hand often harmful to plant to solvent, plant cell can be dissolved, on the other hand,
Solvent for use is generally polar solvent, and after solvent is absorbed, pesticide activity component easily crystallizes precipitation, to influence drug effect.Separately
The general weakly acidic or alkalescent of outer solvent, frequently can lead to active ingredient degradation in long-term storage, and solvent dissolves packing timber
Material etc. various shortcomings, and do not have to solvent, then optional pesticide activity component range is just extremely narrow, be only limitted to water soluble pesticide activity at
Point, and can not achieve prevention and treatment Epidemic Disease of Forest, realize the corresponding plants protection purpose of forest.
Summary of the invention
The shortcomings that in order to overcome above-mentioned trunk injection liquor, the present invention take following technical solution:
A kind of trunk injection liquor for the containing graphene nano material preventing and treating diaphorina citri, the trunk injection liquor include
The raw material of following mass percent:
Active constituent: 0.2-20.0, graphene nano material: 0.1-30, decentralized medium 50-95；The active constituent with
Weight ratio 100:10~100:500 of the graphene nano material, the active constituent includes nicotinic insecticide, the cigarette
Bases insecticide is selected from imidacloprid, Diacloden, clothianidin, dinotefuran, Acetamiprid, thiacloprid, Nitenpyram, pymetrozine, fluorine pyridine
One of worm amine nitrile, flonicamid or several combinations, the decentralized medium are water.
Preferably, the active constituent further includes antibiotics fungicide, and it includes ammonia that the antibiotics fungicide, which is selected from,
Parasiticin, R-77-3, streptomysin, kasugarnycin, terramycin, jinggangmeisu, Ningnanmycin, golden core are mould
One of plain, new phytomycin or several combinations.
Preferably, the graphene nano material includes the derivative of graphene and graphene.
Further, the derivative of the graphene is graphene oxide and its derivative.
Further, the graphene oxide includes the graphene oxide of nano graphene oxide and chemical modification；Wherein institute
The graphene oxide for stating chemical modification includes polyethyleneglycol modified graphene oxide, the graphene oxide of sulfonation modification, carboxylic
It is acidified the graphene of modification.
Preferably, the active constituent further include plant growth regulator, amino acid, humic acid, polysaccharide, in microelement
One or several kinds of combinations, the plant growth regulator includes paclobutrazol.
Preferably, the trunk injection liquor further includes having to soak chemosmotic surfactant, including azone, rouge
One of fat alcohol polyoxyethylene ether, alkyl succinate, alkyl phenol polyoxyethylene ether, organosilicon or several combinations.
Above-mentioned trunk injection liquor is for preventing and treating diaphorina citri, to prevent Citrus Huanglongbing pathogen.
The preparation method of above-mentioned trunk injection liquor, includes the following steps:
A) a certain amount of active constituent is taken, is dissolved in organic solvent or water and obtains active ingredient solution；
B) aqueous solution of containing graphene material and the active ingredient solution are uniformly mixed, mixed liquor is made；
C) organic solvent is distilled off in the mixed liquor, other ingredients is added, be diluted with water to activity component concentration i.e.
Obtain the trunk injection liquor.
The trunk injection liquor of graphene nano material prepared by the present invention after being applied using Stem injection, can effectively prevent
Diversified economy forest pest, is further additionally added fungicide, can also controlling disease while preventing and treating commodity trees pest.In liquid
Growth regulator is added in agent, adjusts the growth of target forest, thus spraying times when reducing commodity trees pest and disease damage, section
About control cost.
Trunk injection liquor decentralized medium of the present invention is water, and graphene nano material has after carrying active constituent
Good water solubility keeps indissoluble, sl. sol. active constituent evenly dispersed in water.Advantages of the present invention is that, by insoluble or
Sl. sol. active constituent is prepared into water soluble preparation, and after the trunk injection liquor of preparation injects citrus layer of wood, active constituent exists
Citrus layer of wood has well conductibility up and down, is easily conducted to citrus bast in terms of horizontal conductive to be transmitted to tree
The top and root of wood.
The trunk injection liquor of graphene nano material prepared by the present invention after being applied using Stem injection, is had only to target
Mark harmful organism is worked, and does not damage citrus to citrus Small side effects without containing solvent, and do not corrode liquid formulation for trunk injection packing timber
Material, it is free from environmental pollution, natural enemy insect is not injured, there is the features such as at low cost, preventive effect is high, applied widely, can be used for anti-Zhiduo County
Kind commodity trees insect pest and disease, realize relevant plant protection purpose.
Specific embodiment
The present invention is described in further detail with reference to embodiments.
Active constituent Diacloden, clothianidin, dinotefuran press hundred Mass Calculations of folding.
Graphene (commercially available, graphene powder is won space high-tech new material technology Co., Ltd by Beijing and provided)
Graphene oxide is made by oneself by Hummers method.
The graphene oxide of sulfonation modification, Carboxylation modification the preparation method of graphene can refer to patent
CN101691204B " stable nano graphene oxide and preparation method thereof under physiological condition "；
The preparation method of polyethyleneglycol modified graphene oxide can refer to document (ginseng YANG K, ZHANG S, et
Al.ACS NANO, 2010).
Embodiment 1
The present embodiment provide prevention and treatment diaphorina citri containing graphene nano material trunk injection liquor obtain preparation step and
Method for testing performance, trunk injection liquor obtained include the raw material of following weight percent:
Active constituent: 0.2-20.0, graphene nano material 0.1-30.Take a certain amount of active constituent such as Diacloden, thiophene
Worm amine, dinotefuran, imidacloprid, Acetamiprid etc. are dissolved in organic solvent (such as acetone, ethyl acetate, sec-butyl acetate, DMF, DMSO
Deng), prepare active ingredient solution.
By graphene aqueous solution or graphene oxide water solution (10mg/mL), with reference to active constituent and graphene nano material
The weight ratio of material is the ratio of 100:(10-500), and mixed solution is made.Mixed solution is placed on draught cupboard, is stirred 24 hours
So that organic solvent is volatilized completely, or be evaporated under reduced pressure out organic solvent, other components are added, are eventually adding water, reaches and need to make
Standby activity component concentration obtains the trunk injection liquid of qualified activity component concentration after mixing, marks respectively.
The trunk injection liquid 0.2mL being prepared is taken, 5mL water is added and is mixed, observes its stability after standing 48 hours,
It takes 1mL to be added in vial again, seals mouth and be placed on 52 ± 2 DEG C standing 14 days in baking oven, observe its stability.
Embodiment 2
10% Acetamiprid streptomysin trunk injection liquor (the weight ratio 1:1 of active constituent and graphene nano material)
20g Acetamiprid is weighed, 200m1 ethyl acetate is slowly added to, is stirred at room temperature 0.5 hour, it is transparent molten to obtain Acetamiprid element
Liquid.
20g streptomysin is added in 100g water and obtains Streptomycin Solution.
Above-mentioned Acetamiprid clear solution is poured into the aqueous solution of 1000mL nano graphene oxide containing 40g, chain is added
It is evaporated under reduced pressure out in draught cupboard organic molten after mycin solution, weighs the weight of surplus solution, water is added and makes active constituent dense
Degree is 10%, obtains 10% Acetamiprid streptomysin trunk injection liquor.
The results show that 10% Acetamiprid streptomysin trunk injection liquor can stable dispersion in aqueous solution, and have it is good
Good thermal stability.
Embodiment 3
The 10% imidacloprid jinggangmeisu trunk injection liquor (weight ratio of active constituent and stannic oxide/graphene nano material
8g imidacloprid 1:0.8) is weighed, DMF 20m1 is slowly added to, is stirred at room temperature 5 minutes, obtains imidacloprid clear solution.
2g jinggangmeisu is added in 20g water, obtains jinggangmeisu solution.
Above-mentioned imidacloprid clear solution is poured into the aqueous solution of 200mL nano graphene oxide containing 8g, Jing Gang is added
Be evaporated under reduced pressure out methanol in draught cupboard after mycin solution, weigh the weight of surplus solution, after add into 10 grams of fatty alcohol polyoxies
Vinethene and 20 grams of polyethers are eventually adding water and activity component concentration are made to be 10%, obtain 10% imidacloprid jinggangmeisu
Trunk injection liquor.
The results show that 10% imidacloprid jinggangmeisu trunk injection liquor can stable dispersion in aqueous solution, and have
Good thermal stability.
Embodiment 4
4.5% emamectin benzoate dinotefuran trunk injection liquor (active constituent and stannic oxide/graphene nano material
Weight ratio 1:0.5)
2g emamectin benzoate is weighed, 20m1 methanol is slowly added to, is stirred at room temperature 5 minutes, obtains emamectin benzoate
Clear solution.
16g dinotefuran is weighed, 50ml DMF is slowly added to, is stirred at room temperature 5 minutes, obtains dinotefuran clear solution.
Above-mentioned emamectin benzoate clear solution is poured into the aqueous solution of 300mL nano graphene oxide containing 9g,
Methanol is evaporated under reduced pressure out in draught cupboard；Dinotefuran clear solution is added in above-mentioned solution again, depressurizes steam in draught cupboard again
Distillate DMF, weigh the weight of solution, after add into 8 grams of fatty alcohol polyoxyethylene ether and 24g polyethers, add and make activity into water
Constituent concentration is 4.5%, obtains 4.5% emamectin benzoate dinotefuran trunk injection liquor.
The results show that 4.5% emamectin benzoate dinotefuran trunk injection liquor can stable dispersion in aqueous solution,
And there is good thermal stability.
Comparative example
Dinotefuran 4g, emamectin benzoate 0.5g, DMF 20g are weighed, methanol complements to 100g.Methanol 5g is taken to dissolve first
Amino avermectin 0.5g, DMF 20g dissolves dinotefuran 4.0g, after stirring is dissolved completely, mixes, adds surface-active
Dissolution is sufficiently stirred in agent fatty alcohol polyoxyethylene ether 2g and 6g polyethers, and methanol is added and is supplemented to 100g to get 4.5% methylamino
Avermectin dinotefuran trunk injection liquor.
Embodiment 5: field controling test
In order to verify the trunk injection liquor of graphene nano material prepared by the present invention to diaphorina citri and citrus Huanglong
The control efficiency of disease, by the trunk injection liquor of graphene nano material obtained in embodiment 2,3,4 and using tradition
Correlation reagent agent made from trunk injection liquor preparation method has carried out citrus trunk injection dry application medicine prevention and treatment diaphorina citri and mandarin orange
The pharmacodynamic test of tangerine yellow twig, specific test result are as follows.
One, the test of the prevention and treatment of trunk injection liquor wood louse and Citrus Huanglongbing pathogen
1 materials and methods
1.1 for reagent tool
1.1.1 medicament
Trunk injection liquor:
Embodiment 2-4 and comparative example gained medicament
1.1.2 medical instruments
Portable rechargeable-type electric hand drill, trunk injection liquor syringe etc..
1.2 for examination tree species and controlling object
1.2.1 for trying tree species
Test site is Hangzhou Jiande City, phoenix village orchard, the town San Dou, and kind is that the age of tree 6 years, spacing in the rows 2-3m, trunk existed
1.5m branch at heights is more.
1.2.2 controlling object
Diaphorina citri and Citrus Huanglongbing pathogen
1.3 test method
On March 26th, 2017, phoenix village orchard navel orange choose 50 in the same size, navel oranges in the consistent susceptible region of the age of tree
Tree carries out yellow twig detection, as a result confirms that these navel orange trees infect Citrus Huanglongbing pathogen, disease incidence 100%.
Progress on April 2nd, 2017 tree trunk injection, the base portion with electric drill in tree tilt down 15 apart from ground 20cm or so place
Degree punching, aperture 0.4cm, hole depth about 3-4cm.Self-flowing medicine injection device tip equipped with medicament is cut out, is broken blind mouth with needle
After insert in the hole, make trunk injection liquor syringe that medicament is injected tree body.Every navel orange trees are 0.45g by injection dose standard
A.i., medicament injection post-sampling absorbent cotton seals injection orifice, then uses red paint marker number on trunk.
Experimental design, which follows, randomly selects principle, randomly selects 10 plants and is used as a processing group, sets 4 processing groups altogether, often
Handle 10 repetitions.Using trunk Stem injection, medicament is inputted, in addition 10 plants of injected clear waters compare.
1.4 drug effect inspections
Every plant of tree randomly selects 10 tender tips on navel orange branch before injection, records diaphorina citri insect population number above, i.e., before medicine
Borer population living, and tied up on petiole with white line so as to repeated observation in the future.Above-mentioned 10 are investigated after 5,10,15 and 20 days after medicament injection
Branch tender tip record diaphorina citri insect population number above, i.e., borer population living after medicine, and observe injection orifice whether there is or not infection phenomenons, navel orange trees whether there is or not
The weak phenomenon of growing way.
Diaphorina citri preventive effect calculation formula is as follows:
Borer population × 100 living before Revision insect recluced rate (%)=(borer population living after borer population-medicine of living before medicine)/medicine
Rectifying efficacies (%)=(treatment region Revision insect recluced rate-check plot Revision insect recluced rate)/(check plot 100- insect population decline
Rate) × 100
Navel orange tender tip is acquired before injection into holes as sample, every month, sampling was primary, carried out after the sample extraction DNA of acquisition
Real time fluorescence quantifying PCR method measures the change conditions of Huanglong's germ Ct value quantity of plant.Realtime fluorescent quantitative PCR experiment ginseng
According to document Wenbin Li et al., 2006.
1.5 results and analysis
Medicament the results are shown in Table 1 to the controlling experiment of diaphorina citri.
It can be seen from Table 1 that 2-4 of embodiment of the present invention medicament has apparent control efficiency to diaphorina citri, it is good
In reference examples medicament；Wherein reach 93.4% after the correcting controling effect 40d of the trunk injection liquor of embodiment 4, is higher than control
Example liquid formulation for trunk injection correcting controling effect 60.5%, this data illustrates that graphene has apparent increasing to active constituent in medicament of the present invention
Effect acts on, and emamectin benzoate may be by Uptake and translocation into tender tip in reference examples.And embodiment 2-4 is handled, and 10
There is diaphorina citri constantly dead after it, and dead insect population number is decreased obviously reference examples after 10 days, illustrates tree prepared by the present invention
The wooden liquid formulation for trunk injection has certain sustained release performance, there is preferable lasting effect.
1 trunk injection liquor of table prevents and treats diaphorina citri pharmacodynamic test
PCR method measures the change conditions of Huanglong's germ Ct value quantity of plant, the results are shown in Table 2, as a result, it has been found that embodiment 2
With the Huanglong's germ Ct value and yellow twig bacteria concentration value of embodiment 3 and control group (CK) before treatment without significant difference (P >
0.05), two groups of Ct value significant difference increase ((P < 0.05) after processing 30 days, 60 days, 90 days and 120 days.And implement
Example 4 and reference examples and control group (CK) are without significant difference (P > 0.05).Illustrate, antibiotic composition is added in medicament of the present invention, it is right
Citrus Huanglongbing pathogen germ shows to obtain inhibiting effect.
2 Citrus Huanglongbing pathogen bacterium CT value of table changes with time
| Medicament | 0d | 30d | 60d | 90d | 120d |
| Embodiment 2 | 33.06±1.02 | 37.32±0.48 | 38.54±0.85 | 38.83±0.66 | 37.97±1.01 |
| Embodiment 3 | 33.09±0.80 | 36.14±0.68 | 37.31±1.11 | 38.04±0.76 | 37.26±0.90 |
| Embodiment 4 | 32.90±0.85 | 32.64±1.86 | 34.13±1.34 | 34.72±0.55 | 34.35±0.85 |
| Reference examples | 34.27±1.08 | 33.65±0.58 | 32.06±0.54 | 33.81±0.80 | 34.34±0.68 |
| CK | 32.72±0.60 | 34.25±0.94 | 33.26±0.88 | 34.23±1.33 | 33.28±1.12 |
Yellow twig bacteria concentration pathogen Concentration Testing the results are shown in Table 3 in citrus tender tip sample, it can be seen that embodiment 2 and reality
It applies after example 3 handles, compared with CK, at 0 day, difference is not significant (P > 0.05), but after 30 days, 60 days, 90 days and 120 days
Significant difference increases, and pathogen concentration value significantly reduces ((P < 0.05).Embodiment 4 and reference examples are compared with CK, pathogen
Concentration value does not significantly reduce ((P > 0.05).Show that antibiotic post-processing navel orange plant in spite of illness is added in liquid formulation for trunk injection of the present invention,
There is preferable effect to the yellow twig bacteria concentration for inhibiting citrus sick tree.And antibiotic is added in liquid formulation for trunk injection of the present invention and post-processes band
Sick navel orange plant has preferable effect to the yellow twig bacteria concentration for inhibiting citrus sick tree.
Yellow twig bacteria concentration * 10 in 3 citrus tender tip sample of table4The change of (pathogen number/every gram of citrus disease sample) at any time
Change
It was found that the navel orange branch for its processing occur occurs volume there are certain phytotoxicity phenomenon after reference examples liquid formulation for trunk injection use
Leaf, withered phenomenon, the symptom of chemical damage that individual navel orange branches also occur are obvious.Know traditional trees fluid injection agent due to system
In contain polar solvent, decentralized medium is organic solvent, it is understood that there may be drug safety hidden danger.Decentralized medium of the present invention is water, this
Prevention and treatment of the liquid formulation for trunk injection of inventive embodiments 2-4 for pest on navel orange, no phytotoxicity phenomenon, and compared with the control group, control
Group endangers later period leaf due to the influence of pest, and almost all crimps, and 2-4 trunk injection liquor of the embodiment of the present invention, place
Reason group navel orange blade face is open and flat, healthy growth, illustrates that the long control effect of medicament is ideal.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, is all covered by the present invention.
Claims (9)
1. a kind of trunk injection liquor for the containing graphene nano material for preventing and treating diaphorina citri, which is characterized in that the trees note
Dry liquor includes the raw material of following mass percent:
Active constituent 0.2-20.0, graphene nano material 0.1-30, decentralized medium 50-95；The active constituent and the stone
The weight ratio of black alkene nano material is 100:10~100:500, and the active constituent includes nicotinic insecticide, the nicotinoids
Insecticide is selected from imidacloprid, Diacloden, clothianidin, dinotefuran, Acetamiprid, thiacloprid, Nitenpyram, pymetrozine, flonicamid
One of nitrile, flonicamid or several combinations, the decentralized medium are water.
2. trunk injection liquor according to claim 1, which is characterized in that the active constituent further includes that antibiotics kill
Microbial inoculum, including ampicillin, R-77-3, streptomysin, kasugarnycin, terramycin, jinggangmeisu, Ningnan are mould
One of element, gold alloy hydride, new phytomycin or several combinations.
3. trunk injection liquor according to claim 1, which is characterized in that the graphene nano material includes graphene
And the derivative of graphene.
4. trunk injection liquor according to claim 3, which is characterized in that the derivative of the graphene is graphite oxide
Alkene and its derivative.
5. trunk injection liquor according to claim 4, which is characterized in that the graphene oxide includes nano oxygen fossil
Black alkene, chemical modification graphene oxide；The graphene oxide of the chemical modification includes polyethyleneglycol modified graphite oxide
Alkene, the graphene oxide of sulfonation modification, Carboxylation modification graphene oxide.
6. trunk injection liquor according to claim 1 or 2, which is characterized in that the active constituent further includes plant growth tune
One of agent, amino acid, humic acid, polysaccharide, microelement or several combinations are saved, the plant growth regulator includes more
Imitate azoles.
7. trunk injection liquor according to claim 1, which is characterized in that the trunk injection liquor further includes having profit
Wet chemosmotic surfactant is selected from azone, fatty alcohol polyoxyethylene ether, alkyl succinate, alkylphenol-polyethenoxy
One of ether, organosilicon or several combinations.
8. a kind of trunk injection liquor according to any one of claim 1 to 7, which is characterized in that the trunk injection
Liquor is for preventing and treating diaphorina citri, to prevent Citrus Huanglongbing pathogen.
9. a kind of trunk injection liquor according to any one of claim 1 to 7, which is characterized in that the trunk injection
The preparation method of liquor includes the following steps:
A) a certain amount of active constituent is taken, is dissolved in organic solvent or water and obtains active ingredient solution；
B) aqueous solution of containing graphene material and the active ingredient solution are uniformly mixed, mixed liquor is made；
C) after organic solvent being distilled off in the mixed liquor, other components are added, are diluted with water to after activity component concentration i.e.
Obtain the trunk injection liquor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811644558.8A CN109430258A (en) | 2018-12-29 | 2018-12-29 | A kind of trunk injection liquor for the containing graphene nano material preventing and treating diaphorina citri |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811644558.8A CN109430258A (en) | 2018-12-29 | 2018-12-29 | A kind of trunk injection liquor for the containing graphene nano material preventing and treating diaphorina citri |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109430258A true CN109430258A (en) | 2019-03-08 |
Family
ID=65539916
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811644558.8A Pending CN109430258A (en) | 2018-12-29 | 2018-12-29 | A kind of trunk injection liquor for the containing graphene nano material preventing and treating diaphorina citri |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109430258A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110089328A (en) * | 2019-05-16 | 2019-08-06 | 郑建秋 | The method for eradicating Citrus Huanglongbing pathogen |
| CN110741854A (en) * | 2019-11-12 | 2020-02-04 | 西南大学 | Method for reducing content of CLas in pathogenic bacteria of citrus Huanglongbing disease |
| CN112369421A (en) * | 2020-11-27 | 2021-02-19 | 中国热带农业科学院椰子研究所 | Combined medicine for protecting plants from damage of rhynchophorus ferrugineus |
| CN117802164A (en) * | 2023-12-29 | 2024-04-02 | 华中农业大学 | Yellow dragon disease control method based on plant inner prokaryotic gene silencing system |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6287503A (en) * | 1985-10-15 | 1987-04-22 | Ube Ind Ltd | Agent for preventing pine blight |
| CN105746566A (en) * | 2016-03-29 | 2016-07-13 | 西北农林科技大学 | Dinotefuran-containing liquid agent for being infused into tree trunks |
| CN106577644A (en) * | 2016-11-25 | 2017-04-26 | 广西田园生化股份有限公司 | Medical fertilizer containing graphene nano material and preparation method of medical fertilizer |
| CN106719627A (en) * | 2016-11-25 | 2017-05-31 | 广西田园生化股份有限公司 | The pesticidal preparations and its preparation method of a kind of sustained-release pesticides composition and its composition |
-
2018
- 2018-12-29 CN CN201811644558.8A patent/CN109430258A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6287503A (en) * | 1985-10-15 | 1987-04-22 | Ube Ind Ltd | Agent for preventing pine blight |
| CN105746566A (en) * | 2016-03-29 | 2016-07-13 | 西北农林科技大学 | Dinotefuran-containing liquid agent for being infused into tree trunks |
| CN106577644A (en) * | 2016-11-25 | 2017-04-26 | 广西田园生化股份有限公司 | Medical fertilizer containing graphene nano material and preparation method of medical fertilizer |
| CN106719627A (en) * | 2016-11-25 | 2017-05-31 | 广西田园生化股份有限公司 | The pesticidal preparations and its preparation method of a kind of sustained-release pesticides composition and its composition |
Non-Patent Citations (1)
| Title |
|---|
| 崔永亮 等: "猕猴桃溃疡病防治药剂筛选及田间防效", 《安徽农业科学》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110089328A (en) * | 2019-05-16 | 2019-08-06 | 郑建秋 | The method for eradicating Citrus Huanglongbing pathogen |
| CN110741854A (en) * | 2019-11-12 | 2020-02-04 | 西南大学 | Method for reducing content of CLas in pathogenic bacteria of citrus Huanglongbing disease |
| CN110741854B (en) * | 2019-11-12 | 2021-10-26 | 西南大学 | Method for reducing content of pathogenic bacteria CLas of citrus huanglongbing |
| CN112369421A (en) * | 2020-11-27 | 2021-02-19 | 中国热带农业科学院椰子研究所 | Combined medicine for protecting plants from damage of rhynchophorus ferrugineus |
| CN112369421B (en) * | 2020-11-27 | 2022-07-22 | 中国热带农业科学院椰子研究所 | Combined medicine for protecting plants from damage of rhynchophorus ferrugineus |
| CN117802164A (en) * | 2023-12-29 | 2024-04-02 | 华中农业大学 | Yellow dragon disease control method based on plant inner prokaryotic gene silencing system |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109430258A (en) | A kind of trunk injection liquor for the containing graphene nano material preventing and treating diaphorina citri | |
| CN102578164B (en) | Compounded botanical synergistic pesticide containing sophocarpidine and macleaya alkaloids and preparation method of compounded botanical synergistic pesticide | |
| CN103609575B (en) | A kind of bactericidal composition containing tetramycin and pyraclostrobin | |
| CN103960228A (en) | Pesticide film forming agent used for controlling diseases and insect pests of forest trees, and its preparation method | |
| CN109430257A (en) | A kind of injection of containing graphene nano material that preventing pine nematode | |
| CN104488998A (en) | Synergistic compound hicide caphidicide containing sophocarpidine and preparation method of synergistic compound hicide caphidicide | |
| CN102283255B (en) | Nicotine-containing botanical synergist compound insecticide and preparation method thereof | |
| CN102028001B (en) | Sophora alopecuroides L alkaloid-containing plant-derived synergistic compound aphicide and preparation method thereof | |
| CN103907612B (en) | Pyriminostrobin-containing insecticidal composition | |
| CN106797951B (en) | A kind of dry insecticide of note and its preparation method and application for preventing and treating corythucha ciliate | |
| CN105145663A (en) | Botanical pesticide composition containing azadirachtin and eucalyptus oil | |
| CN102007944B (en) | Plant source synergistic compound aphicide and acaricide containing rotenone and preparation method thereof | |
| CN101843268B (en) | Botanical synergistic compound aphicide and preparation method thereof | |
| CN109497048A (en) | A kind of trunk injection liquor of containing graphene nano material | |
| CN102239891B (en) | Enhanced compound pesticide containing toosedarin plant source and preparation method thereof | |
| KR101162882B1 (en) | Agents for exterminating and controlling bursaphelenchus lignicolus and method for manufacturing thereof, and method for controlling witheredness of pine tree by bursaphelenchus lignicolus | |
| CN113875764A (en) | Emamectin benzoate soluble solution, preparation method and application thereof | |
| CN114190395A (en) | Application of bactericidal composition containing tetramycin and oxine-copper in preventing and treating plant xanthomonas diseases | |
| CN102669124A (en) | Biological source compound insecticide and application thereof | |
| CN108042558B (en) | Abamectin preparation for aquaculture and preparation method thereof | |
| CN103931649A (en) | Insecticidal composition containing emamectin benzoate and chlorempenthrin | |
| CN105409996B (en) | A kind of herbicidal composition containing sulfometuronmethyl and MCPA,MCP,methoxone sodium | |
| CN105875610A (en) | Application of Acnistus A in insecticide preparation | |
| CN106900756B (en) | Insecticidal composition of pueraria peduncularis extract and azadirachtin for preventing and treating agricultural pests and application thereof | |
| CN106386821A (en) | Pesticide composition containing 2-methyl sulfone-5-(2,4-dichlorophenyl)-1,3,4-oxadiazole and difenoconazole |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190308 |
|
| RJ01 | Rejection of invention patent application after publication |