CN112114013A

CN112114013A - Multi-channel biochemical detection test piece

Info

Publication number: CN112114013A
Application number: CN201910531128.3A
Authority: CN
Inventors: 黄国财; 张国忠
Original assignee: Guo Hoong Industry Co ltd
Current assignee: Guo Hoong Industry Co ltd
Priority date: 2019-06-19
Filing date: 2019-06-19
Publication date: 2020-12-22
Anticipated expiration: 2039-06-19
Also published as: CN112114013B

Abstract

The invention discloses a multi-channel biochemical detection test piece which comprises a substrate, a circuit layer, a plurality of detection reagents and a cover plate. The substrate comprises a reading section and a detection section, wherein a convex seat and a detection channel are formed on the surface of the detection section, the convex seat is provided with a groove higher than the detection channel and a plurality of grooves communicated with the groove, and the grooves are respectively correspondingly connected with the detection channel; the circuit layer is arranged on the substrate and comprises a plurality of metal lines and a plurality of electrodes, the metal lines extend from the reading section to the detection section, and the electrodes are exposed in the detection channel; the detection reagent is arranged in the detection channel and covers the electrode; the cover plate is combined on the detection section, covers the detection channel and exposes out of the groove of the boss; therefore, various measurement items can be detected simultaneously, and mixing and current conduction of the objects to be detected in each detection flow channel can be avoided, so that the detection result of each detection flow channel is ensured.

Description

Multi-channel biochemical detection test piece

Technical Field

The present invention relates to a biochemical detection device, and more particularly, to a biochemical detection test strip.

Background

The biochemical detection device is used for acquiring physiological information of an analyte to be detected and is used as a reference basis for analyzing the analyte. Most of the current biosensor test strips are provided with a detection opening and a detection flow channel on a plastic sheet, so that an object to be detected is dropped into the detection opening and flows into the detection flow channel, and then the object is measured to obtain related data.

The conventional biosensor test strip only has a single detection flow channel, so that only one value can be measured, and if a plurality of values are measured, the object to be measured must be collected for a plurality of times, which not only results in a long detection time, but also results in waste due to the use of a plurality of detection sheets. Furthermore, although there are biosensing test pieces with a plurality of detecting flow channels, the detecting flow channels of the biosensing test piece have small space, and the detecting object inlet and the detecting flow channel are in the same plane without potential difference. Therefore, in the detection process of the object to be detected, the sensing test piece is easy to overflow or reverse flow and the like only under the condition of dynamic shaking or non-horizontal test piece, so that the objects to be detected in each detection flow channel are likely to be mixed or communicated, and the current can be conducted with each other during detection to influence the detection result.

In view of the above, the present inventors have made extensive studies and studies to solve the above problems in combination with the application of the above prior art, and as a result, the present inventors have improved the present invention.

Disclosure of Invention

An objective of the present invention is to provide a multi-channel biochemical test strip, which can simultaneously detect a plurality of measurement items with a small amount (one drop) of the object to be detected, and can avoid the mixing of the objects to be detected in each detection channel and the mutual conduction interference of the currents between the channels, so as to ensure the detection results of each detection channel.

In order to achieve the above object, the present invention provides a multi-channel biochemical test strip, which includes a substrate, a circuit layer, a plurality of detection reagents, and a cover plate. The substrate comprises a reading section and a detection section, wherein a convex seat and a detection channel are formed on the surface of the detection section, the convex seat is provided with a groove higher than the detection channel and a plurality of grooves communicated with the groove, and the grooves are respectively correspondingly connected with the detection channel; the circuit layer is arranged on the substrate and comprises a plurality of metal lines and a plurality of electrodes, the metal lines extend from the reading section to the detection section, and the electrodes are exposed in the detection channel; the detection reagent is arranged in the detection channel and covers the electrode; the cover plate is combined on the detection section, covers the detection channel and exposes out of the groove of the boss.

Wherein, the detection channels are radially arranged on the outer periphery of the boss.

The convex seat is provided with a separating block which is higher than the bottom surface of the groove between the grooves, one end of the separating block is connected with the groove, and the other end of the separating block extends between the adjacent detection channels.

Wherein, the surface of the groove and/or the groove has a capillary hydrophilic structure.

The groove is communicated with the detection channels, and a detention groove lower than the bottom surface of the groove is formed in one side of each detection channel communicated with the groove.

Wherein, the detention groove is connected with the detection channel by a first inclined plane and connected with the groove by a second inclined plane.

And a first convex surface and a second convex surface which are in a ladder shape are formed on one side of each detection channel connected with the detention groove, and the second convex surface is higher than the first convex surface.

Each detection channel is a U-shaped groove, and a flange is formed on the periphery of each detection channel and is higher than the surface of the detection section.

Wherein, the surface of the flange is coated with a glue layer for adhering the cover plate.

Wherein, the metal circuit is formed with a plurality of wave sections on one side of the electrode, and the wave sections are positioned below the bottom of the detection channel.

Compared with the prior art, the substrate of the multi-channel biochemical detection test piece is provided with the boss and the independent detection channels, so that multiple detections can be performed simultaneously; in addition, the convex seat is provided with a groove and a plurality of grooves which are higher than the detection channels, so that objects to be detected can smoothly flow into the detection channels without the assistance of external power, in addition, the grooves have buffer characteristics and can be used as stagnation grooves for containing the objects to be detected and bubbles which may be generated, so that the objects to be detected can generate shunting along the grooves of the convex seat after being dripped into the grooves and evenly enter the detection channels, and after the cover plate is covered, the objects to be detected can flow and be attached to the detection reagent due to the capillary phenomenon and the siphon principle in the detection channels, therefore, the objects to be detected in the adjacent detection channels cannot be mixed due to overflow, and the current among the channels can be mutually conducted and interfered, so that the detection result correctness of each detection channel is ensured.

The invention is described in detail below with reference to the drawings and specific examples, but the invention is not limited thereto.

Drawings

FIG. 1 is a schematic three-dimensional appearance diagram of the multi-channel biochemical test strip of the present invention

FIG. 2 is a partially enlarged view of FIG. 1

FIG. 3 is a schematic view of the attachment of the patch of the present invention;

FIG. 4 is a schematic diagram showing the three-dimensional appearance of the circuit layer of the present invention

FIG. 5 is a cross-sectional view of the multi-channel biochemical test strip of the present invention

FIG. 6 is a partially enlarged view of the multi-channel biochemical test strip of the present invention;

FIG. 7 and FIG. 8 are a schematic view and a cross-sectional view of the multi-channel biochemical test strip of the present invention dropping a substance to be detected;

FIG. 9 is a schematic flow diagram of an analyte of the present invention;

fig. 10 is another embodiment of a circuit layer of the present invention.

FIG. 11 shows another embodiment of the multi-channel biochemical test strip of the present invention.

Wherein, the reference numbers:

1 … multi-channel biochemical test strip

2 … capillary tube

3 … object to be detected

10 … base plate

100 … circuit positioning hole

11 … read segment

12 … detection segment

121. 121a … boss

1210 … groove

1211 … groove

1212 … divider block

122 … detection channel

1220 … detention tank

1221 … Flange

1222 … first inclined plane

1223 … second inclined plane

1224 … first convex surface

1225 … second convex surface

13 … first positioning part

20 … Circuit layer

20' … tape connecting section

21. 21a … metal line

211 … wave segment

22 … electrode

30 … detection reagent

40 … cover sheet

41 … second locator portion

42 … hollowed-out part

Detailed Description

The following detailed description and technical contents of the present invention are described with reference to the drawings, which are provided for reference and illustration purposes only and are not intended to limit the present invention.

Referring to fig. 1 to fig. 4, a schematic three-dimensional appearance diagram, a schematic partially enlarged diagram, a schematic combination diagram of a cover sheet, and a schematic three-dimensional appearance diagram of a circuit layer of the multi-channel biochemical detection test strip of the present invention are respectively shown. The present invention relates to a multi-channel biochemical test strip 1, which comprises a substrate 10, a circuit layer 20, a plurality of detection reagents 30 and a cover sheet 40. The substrate 10 is combined with the circuit layer 20 by an integral injection molding, the detecting reagents 30 are disposed on the electrodes 22, and the cover sheet 40 is combined on the substrate 10 to cover the detecting reagents 30, so as to form the multi-channel biochemical detecting test piece 1, which will be described later.

As shown in fig. 1 and fig. 2, the substrate 10 includes a reading section 11 and a detecting section 12, and a boss 121 and a plurality of detecting channels 122 are formed on the surface of the detecting section 12 of the substrate 10. The boss 121 has a groove 1210 higher than the detection channels 122 and a plurality of grooves 1211 communicating with the groove 1210, and the grooves 1211 are respectively connected to the detection channels 122. For example, the substrate 10 may be integrally injection-molded by plastic material of 20% Glass Fiber (FB) mixed with Polybutylene terephthalate (PBT), thereby increasing the precision of the substrate 10, increasing the yield and reducing the cost.

Specifically, the detecting channels 122 are radially arranged on the outer periphery of the boss 121, and the detecting channels 122 are straight without bending, so that the object to be detected can smoothly flow in the detecting channels 122 without being blocked. Preferably, a separating block 1212 higher than the bottom surface of the groove 1210 is formed between the grooves 1211 of the boss 121, and one end of the separating block 1212 is connected to the groove 1210 and the other end extends between the adjacent detecting channels 122, so as to prevent the objects to be detected in the adjacent detecting channels 122 from overflowing and mixing.

In this embodiment, each of the detecting channels 122 is a U-shaped groove, the left and right inner side surfaces of the U-shaped groove have capillary hydrophilic structures (not shown), such as micro-scale rough surfaces, etc., the arrangement of the hydrophilic structures facilitates the flow of the object to be detected for detection, and a flange 1221 is formed at the periphery of each of the detecting channels 122, and in addition, the flange 1221 is higher than the surface of the detecting section 12 to prevent the object to be detected in the detecting channel 122 from overflowing. In addition, the surface of the flange 1221 is a smooth surface coated with a glue layer (not shown) for adhering the cover sheet 40.

It is noted that the substrate 10 is formed with a plurality of circuit positioning holes 100, and the circuit positioning holes 100 are used as pressing points in the injection molding process to position the circuit layer 20.

Referring to fig. 4, the circuit layer 20 is disposed on the substrate 10. The circuit layer 20 includes a plurality of metal lines 21 and a plurality of electrodes 22 disposed on the metal lines 21. In an embodiment of the present invention, the number of the metal lines 21 and the electrodes 22 is six, and the electrodes 22 are disposed in a cylindrical shape; however, the number and shape of the metal lines 21 and the electrodes 22 are not limited in practical use and can be adjusted and changed according to practical use conditions.

Furthermore, the metal lines 21 extend from the reading section 11 of the substrate 10 to the detecting section 12, and the electrodes 22 are exposed in the detecting channel 122 of the detecting section 12, and are manufactured by punching, drawing, and pressing to increase the product precision and the bonding area, thereby increasing the bonding force between the electrodes and the substrate 10 and preventing the detection reagent 30 from leaking during the manufacturing process.

In the present embodiment, the circuit layer 20 is made of other noble metals such as brass. In addition, the metal lines 21 are processed by nickel electroplating, and the electrodes 22 are further processed by other processes, such as nickel electroplating on the surfaces of the electrodes 22, or gold electroplating or carbon coating on the outer layer of the nickel electroplating.

The detecting reagents 30 are disposed in the detecting channels 122 and cover the electrodes 22, and in actual use, the detecting reagents 30 are disposed in the detecting channels 122 in a solid crystal form, and the types of the detecting reagents 30 are set according to the detecting items. It should be noted that the detection channels 122 are rough before the detection reagents 30 are disposed, so as to increase the adhesion force to the detection reagents 30 by the rough surface.

The cover sheet 40 is attached to the inspection section 12 of the base plate 10. The cover plate 40 covers the detecting channels 122 and exposes the grooves 1210 of the boss 121, so that the object to be tested can drop into the grooves 121 and flow into the detecting channels 122, and the object to be tested can be prevented from overflowing from the detecting channels 122.

More specifically, the cover plate 40 may be a transparent film to facilitate observing the object flowing into the detecting channels 122. In addition, in an embodiment of the present invention, the detecting section 12 of the substrate 10 has a plurality of first positioning portions 13, the cover 40 has a plurality of second positioning portions 41 on a surface facing the substrate 10, and the substrate 10 and the cover 40 are combined by mutually positioning the positioning portions 13 and the second positioning portions 41. In addition, the cover plate 40 is further provided with a hollow portion 42 corresponding to the position of the boss 121, and the cover plate 40 has a hydrophilic film (not shown) with hydrophilic functional group substances on the surface facing the detection channels 122 to assist the flow of the analyte.

It should be noted that the surfaces of the groove 1210, the grooves 1211, and the inner sides (not shown) of the U-shaped grooves have capillary hydrophilic structures, such as micro-scale rough surfaces, and the hydrophilic films on the cover 40 are further mounted, so that the object 3 can rapidly flow into the detection channel 122, and the arrangement of the hydrophilic structures is favorable for the flow of the object to be detected for detection.

Referring to fig. 4, it should be noted that the circuit layer 20 of the present invention is disposed in a tape type and has a tape connecting section 20', so as to facilitate the simultaneous processing and production of a plurality of circuit layers 20, thereby reducing the production cost and increasing the yield. And the circuit layer 20 and the substrate 10 are combined by means of integral injection molding. Therefore, the tape connecting section 20' of the circuit layer 20 is not removed in advance, and is integrally injection-molded with the substrate 10. In addition, the number of the detecting channels 122 of the present invention is designed according to the actual usage situation by matching with the circuit layer 20, and the cover sheet 40 also needs to be designed accordingly.

Referring to fig. 5 and fig. 6, a cross-sectional view and a partial enlarged view of the multi-channel biochemical test strip of the present invention are shown. In this embodiment, the groove 1210 is connected to the detecting channels 122, and a retention groove 1220 is formed between each detecting channel 122 and one side of the groove 1210 connected to the detecting channel 122 and is lower than the bottom surface of the groove 1210.

As shown in FIG. 6, the retention groove 1220 is connected to the detection channel 122 by a first slope 1222 and to the recess 1210 by a second slope 1223. In addition, referring to fig. 2, in the embodiment, each of the detecting channels 122 is formed with a first convex surface 1224 and a second convex surface 1225 in a step shape at a side connecting with the retention groove 1220, and the first convex surface 1224 is disposed with the detecting reagent 30 slightly higher than the surface of the electrodes 22, so as to ensure that the object to be detected flows through the electrodes 22; in addition, the second convex surface 1225 is higher than the first convex surface 1224 to prevent overflow of the detection reagent 30 during the process of filling the detection channel 122.

It should be noted that, in an embodiment of the present invention, the metal line 21 of the circuit layer 20 is formed with a plurality of wave segments 211 at one side of the electrodes 22, and the wave segments 211 are located below the bottoms of the detection channels 122 to increase the bonding area between the metal lines 21 and the substrate 10, thereby preventing the detection reagent 30 from leaking.

Fig. 7 to 9 are a schematic view, a cross-sectional view, and a schematic flow diagram of the multi-channel biochemical test strip of the present invention. As shown in FIG. 7 and FIG. 8, when the multi-channel biochemical test strip 1 of the present invention is used, a capillary 2 is first used to suck a substance 3 to be tested, and then the capillary 2 is moved to a position above the boss 121, and the substance 3 to be tested is dropped into the groove 1210.

As shown in fig. 9, the object 3 dropped into the groove 1210 flows along the groove 1211 of the protrusion 121 and enters into each detection channel 122 evenly, and then the object 3 reacts with the detection reagent 30 in the detection channel 122, and finally the reaction result and data are transmitted to the metal lines 21 through the electrodes 22 and then transmitted to the outside through the metal lines 21.

It is noted that, in the present invention, the object 3 to be detected firstly flows through the second inclined surface 1223 and falls into the retention groove 1220 to generate a pressure difference of potential energy, and flows into the detection channel 122 through capillary and siphon phenomena to react with the detection channel 122. In addition, because the height difference exists between the retention groove 1220 and the groove 1210, the excessive objects 3 to be detected will stagnate in the retention groove 1220 and will not flow back to the groove 1210, and in addition, the design of the depression of the retention groove 1220 can accommodate the possible bubbles, so that the bubbles can be prevented from flowing into the detection channels 122, thereby achieving the buffering effect and avoiding the situation that the objects 3 to be detected in each detection channel 122 flow back to cause mixed pollution and the current of each flow channel is conducted mutually.

Referring to fig. 10, another embodiment of the circuit layer of the present invention is shown. In this embodiment, the circuit layer 20 includes a plurality of metal lines 21 and a plurality of electrodes 22 disposed on the metal lines 21. The present embodiment is different from the previous embodiment mainly in the number of metal lines 21 and the arrangement of the electrodes 22. In the present embodiment, the number of the metal lines 21 and the electrodes 22 is ten, and the electrodes 22 are disposed in a rectangular shape.

Referring to FIG. 11, another embodiment of the multi-channel biochemical test strip of the present invention is shown. In this embodiment, the multi-channel biochemical test strip 1 includes a substrate 10, a circuit layer 20, a plurality of detecting reagents 30, and a cover sheet 40, and the substrate 10 is combined with the circuit layer 20 by an integral injection molding. The main difference between this embodiment and the previous embodiment lies in the circuit layer 20 (described above) and the substrate 10.

In the present embodiment, the substrate 10 is formed with a boss 121 and a plurality of detection channels 122, and in the present embodiment, the number of the detection channels 122 is five according to the circuit layer 20.

The above description is only for the preferred embodiment of the present invention, and is not intended to limit the scope of the present invention. The present invention is capable of other embodiments, and various changes and modifications may be made by one skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims.

Claims

1. A multi-channel biochemical test strip, comprising:

the substrate comprises a reading section and a detection section, wherein a convex seat and a plurality of detection channels are formed on the surface of the detection section of the substrate, the convex seat is provided with a groove higher than the detection channels and a plurality of grooves communicated with the groove, and the grooves are respectively correspondingly connected with the detection channels;

a circuit layer disposed on the substrate, the circuit layer including a plurality of metal lines and a plurality of electrodes disposed on the metal lines, the metal lines extending from the reading section to the detecting section, the electrodes being exposed in the detecting channels of the detecting section;

a plurality of detection reagents arranged in the detection channel and covering the electrodes; and

and the cover plate is combined on the detection section, covers the detection channel and exposes out of the groove of the convex seat.

2. The multi-channel biochemical test strip according to claim 1, wherein the detecting channels are radially arranged on the outer periphery of the boss.

3. The multi-channel biochemical test strip according to claim 1, wherein the convex base is formed with a separation block between the grooves, the separation block is higher than the bottom surface of the groove, one end of the separation block is connected to the groove, and the other end of the separation block extends between adjacent detection channels.

4. The multi-channel biochemical test strip according to claim 1, wherein the surface of the groove and/or the trench has a capillary hydrophilic structure.

5. The multi-channel biochemical test strip according to claim 1, wherein the groove is connected to the detecting channels, and a retention groove lower than the bottom surface of the groove is formed on one side of each of the detecting channels connected to the groove.

6. The multi-channel biochemical test strip of claim 5, wherein the retention groove is connected to the detecting channel by a first inclined plane and connected to the recess by a second inclined plane.

7. The multi-channel biochemical test strip according to claim 5, wherein each of the detecting channels has a first convex surface and a second convex surface formed in a step shape on a side connecting with the retention groove, and the second convex surface is higher than the first convex surface.

8. The multi-channel biochemical test strip according to claim 1, wherein each of the detecting channels is a U-shaped groove, and a flange is formed on the periphery of each of the detecting channels, and the flange is higher than the surface of the detecting section.

9. The multi-channel biochemical test strip according to claim 8, wherein the surface of the flange is coated with a glue layer for adhering the cover sheet.

10. The multi-channel biochemical test strip according to claim 1, wherein the metal circuit has a plurality of wave segments formed on one side of the electrode, and the wave segments are located below the bottom of the detection channel.