KR20150112911A - Biosensor strip and manufacturing method of it - Google Patents

Biosensor strip and manufacturing method of it Download PDF

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Publication number
KR20150112911A
KR20150112911A KR1020150131338A KR20150131338A KR20150112911A KR 20150112911 A KR20150112911 A KR 20150112911A KR 1020150131338 A KR1020150131338 A KR 1020150131338A KR 20150131338 A KR20150131338 A KR 20150131338A KR 20150112911 A KR20150112911 A KR 20150112911A
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KR
South Korea
Prior art keywords
coating layer
electrode layer
layer
electrode
coating
Prior art date
Application number
KR1020150131338A
Other languages
Korean (ko)
Inventor
최성숙
Original Assignee
최성숙
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Application filed by 최성숙 filed Critical 최성숙
Priority to KR1020150131338A priority Critical patent/KR20150112911A/en
Priority to PCT/KR2015/010380 priority patent/WO2017047856A1/en
Publication of KR20150112911A publication Critical patent/KR20150112911A/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/66—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood sugars, e.g. galactose
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors

Abstract

The present invention relates to a biosensor strip which can perform a physical analysis through body fluid such as blood or the like, and a manufacturing method thereof. The biosensor strip comprises: a base sheet made of an insulating material; and an electrode unit formed on an upper part of the base sheet. In the biosensor strip performing a diagnosis through body fluid flowing into the electrode unit, the electrode unit comprises: a first coating layer formed on an upper part of the base sheet; a copper electrode layer formed on an upper part of the first coating layer; a second coating layer formed on an upper part of a first electrode layer, and coated on a sample reaction unit performing a reaction with body fluid; and a gold electrode layer formed on an upper part of the second coating layer.

Description

Technical Field [0001] The present invention relates to a biosensor strip and a manufacturing method thereof,

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a biosensor strip and a method of manufacturing the same, and more particularly, to a biosensor strip capable of performing a body analysis through a body fluid such as blood and a method of manufacturing the same.

Quantitative or qualitative analysis of analytes present in body fluids is of great importance both chemically and clinically. A typical example is the measurement of blood glucose in the blood for diabetic patients or the measurement of cholesterol which is a factor of various adult diseases. It is very important to check the body condition by periodically measuring the blood glucose level in diabetic patients.

As a result, several companies are launching products that measure blood sugar.

Most of the blood glucose measurement products use blood glucose measurement methods and biosensors such as glucose test strips to quantify blood glucose in blood.

In diabetic patients, it is recommended that blood glucose be tested periodically four times a day. Since it is a great burden to the patient to perform blood collection every time the test is performed, it is possible to perform the test only with the minimum amount of blood, , And it is required to derive accurate test results through the collected blood.

Korean Patent Publication No. 10-2010-0042399: Structure of an electrochemical biosensor and measurement method using a biosensor Korean Patent Laid-Open No. 10-2007-0121980: Method for manufacturing electrode for electrochemical biosensor and electrode structure therefor

SUMMARY OF THE INVENTION The present invention has been made in order to solve the above problems, and it is an object of the present invention to provide a biosensor strip capable of improving the reliability of a body analysis measurement value through a body fluid by forming a gold electrode layer on a copper electrode layer in a reaction part, And a method for producing the same.

The present invention relates to a biosensor strip comprising a base sheet of an insulating material and an electrode section formed on the base sheet, the biosensor strip being diagnosed through a body fluid flowing into the electrode section, A second coating layer formed on the first electrode layer and coated on a specimen reaction part where reaction with the body fluids is performed; and a second coating layer formed on the upper part of the second coating layer, And a gold electrode layer.

The first coating layer and the second coating layer may be formed by coating nickel to improve adhesion between the copper electrode layer and the gold electrode layer.

Preferably, the first and second coating layers, the copper electrode layer and the gold electrode layer are formed by vapor deposition, and the copper electrode layer comprises a reference electrode, a working electrode, and a counter electrode.

A method of manufacturing a biosensor strip according to the present invention comprises the steps of: coating a first coating layer on a base sheet of an insulating material; depositing copper on the top surface of the first coating layer; Forming a copper electrode layer on the other side of the copper electrode layer, the copper electrode layer having a reactive portion reacting with the green blood sample, and coating a second coating layer on the upper surface of the reaction portion of the copper electrode layer; and depositing gold on the upper surface of the second coating layer, .

The first coating layer and the second coating layer may be formed by depositing nickel in order to increase adhesion between the copper electrode layer and the gold electrode layer.

According to the biosensor strip of the present invention and the method of manufacturing the same, the electrode portion includes the copper electrode layer and the gold electrode layer. In particular, since the gold electrode layer is formed only in a part of the reaction portion that reacts with the body fluid, There is an advantage.

1 is a perspective view showing an example of the use of the biosensor strip according to the present invention,
FIG. 2 is an exploded perspective view showing an embodiment of the biosensor strip of the present invention, FIG.
3 is a cross-sectional view of the biosensor strip of FIG. 2,
4 is a flowchart showing a method of manufacturing a biosensor strip according to the present invention.

Hereinafter, a biosensor strip according to an embodiment of the present invention and a method of manufacturing the same will be described in detail with reference to the accompanying drawings. The present invention is capable of various modifications and various forms, and specific embodiments are illustrated in the drawings and described in detail in the text. It is to be understood, however, that the invention is not intended to be limited to the particular forms disclosed, but on the contrary, is intended to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the invention. Like reference numerals are used for like elements in describing each drawing. In the accompanying drawings, the dimensions of the structures are enlarged to illustrate the present invention in order to clarify the present invention.

The terms first, second, etc. may be used to describe various components, but the components should not be limited by the terms. The terms are used only for the purpose of distinguishing one component from another. For example, without departing from the scope of the present invention, the first component may be referred to as a second component, and similarly, the second component may also be referred to as a first component.

The terminology used in this application is used only to describe a specific embodiment and is not intended to limit the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In this application, the terms "comprises", "having", and the like are used to specify that a feature, a number, a step, an operation, an element, a part or a combination thereof is described in the specification, But do not preclude the presence or addition of one or more other features, integers, steps, operations, components, parts, or combinations thereof.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in commonly used dictionaries are to be interpreted as having a meaning consistent with the contextual meaning of the related art and are to be interpreted as either ideal or overly formal in the sense of the present application Do not.

As shown in FIG. 1, the biosensor strip according to the present invention is mounted on a measuring instrument 1, and then a body fluid such as blood or saliva is applied to the strip, and then the measured value measured by the measuring instrument 1 is read to measure blood glucose level or cholesterol And so on.

The biosensor strip 2 includes a base sheet 10, an electrode portion 20 formed on the top of the base sheet 10, and an electrode portion 20 formed on an upper portion of the electrode portion 20, as shown in Figs. 2 and 3 And a cover sheet 40 formed on the adhesive sheet 30 and the adhesive sheet 30.

The base sheet 10 may be a PET film used for a biosensor. In this embodiment, a PET film is used as the base sheet 10, but a thin film made of a material such as PVC or polycarbonate A member on the strip may be applied.

An electrode portion (20) is formed on the base sheet (10).

One side of the electrode unit 20 is inserted into the measuring device 1, and the other side is injected with a body fluid to cause a reaction of body fluid for analysis.

The electrode unit 20 includes a first coating layer 21, a copper electrode layer 22, a second coating layer 25, and a gold electrode layer 26.

The first coating layer 21 is formed by depositing nickel on the base sheet 10, which is a PET film. The first coating layer 21 is formed to increase the adhesion of the copper electrode layer 22 to the base sheet 10.

The copper electrode layer 22 is formed of copper on the upper surface of the first coating layer 21.

In the present embodiment, the copper electrode layer 22 is composed of a reference electrode, a working electrode, and a counter electrode. The copper electrode layer 22 is inserted into the measuring device 1 at one side thereof, And a reaction part 24 for reaction with the body fluid is formed on the other side.

The second coating layer 25 is formed by depositing nickel as in the first coating layer 21 and is formed on the reaction part 24 for reaction with the body fluid in the upper part of the copper electrode layer 22. The second coating layer 25 is for enhancing the bonding force between the gold electrode layer 26 and the copper electrode layer 22 to be described later.

Preferably, the first coating layer 21 and the second coating layer 25 are made of nickel of boron type or phosphorus type, and the thickness of the nickel layer is preferably 0.1 to 10 μm.

It is also possible to perform the pretreatment before forming the first coating layer 21 and the second coating layer 25 on the base sheet 10 and the copper electrode layer 22 respectively. Surface treatment can be carried out.

The gold electrode layer 26 is formed by forming an electrode layer on the first coating layer 21 using gold. Since the gold electrode layer 26 is formed on the upper part of the reaction part 24 for the body fluid reaction, the reliability of the measured value can be increased while minimizing the amount of gold used.

The gold electrode layer 26 may be formed by electroless gold plating or electrolytic gold plating. When electroless gold plating is used, it is preferable to use PGC (potassium gold cyanide) 1 占 퐉.

The biosensor strip 2 according to the present embodiment is a sensor for measuring blood glucose. A reaction enzyme 27 is applied to the upper portion of the gold electrode layer 26 and the second coating layer 25 for blood glucose measurement.

The reaction enzyme 27 may be a glucose oxidase. When blood is injected, the blood glucose contained in the blood is oxidized by the reaction enzyme 27, and the reaction enzyme 27 is reduced. The reduced reaction enzyme 27 loses electrons at the surface of the working electrode to which a constant voltage is applied and is oxidized again by electrochemistry. Since the blood glucose concentration of the injected body fluid is proportional to the current generated during the oxidation of the reactive enzyme 27, the blood glucose concentration is measured by measuring the current amount.

The electrode unit 20 of the present invention is formed by sequentially stacking the first coating layer 21, the copper electrode layer 22, the second coating layer 25, and the gold electrode layer 26 sequentially.

The adhesive sheet (30) is attached to the upper portion of the electrode portion (20).

The adhesive sheet 30 may be formed of a double-sided tape having a predetermined thickness for adhering the electrode unit 20 and the cover sheet 40 described below.

The adhesive sheet 30 is cut so as not to cover the reaction part 24 where the copper electrode layer 22 is formed.

The cover sheet 40 is attached to the upper surface of the adhesive sheet 30. The cover sheet 40 protects the electrode unit 20 and may print a name or other related information on the upper surface.

When the adhesive sheet 30 and the cover sheet 40 are attached to each other, a cut portion formed on the adhesive sheet 30 for exposing the gold electrode layer 26 as shown in the sectional view of FIG. 3, An injection groove 50 for injecting body fluid into the space between the cover sheets 40 is formed. The injection groove 50 sucks the body fluid, that is, the blood, on the principle of capillary phenomenon, and the body fluid can flow into the reaction part 24.

A method of manufacturing a biosensor strip according to the present invention is shown in FIG.

Nickel is deposited on top of the base sheet 10 to form a first coating layer 21 and a copper electrode layer 22 is formed on top of the first coating layer 21.

After the copper electrode layer 22 is formed, nickel is deposited on the upper surface of the reaction part 24 through which the body fluid is injected and reacted with the reaction enzyme 27, thereby forming the second coating layer 25.

A gold electrode layer 26 is formed on the upper surface of the second coating layer 25.

After the first coating layer 21, the copper electrode layer 22, the second coating layer 25 and the gold electrode layer 26 are sequentially formed to form the electrode unit 20, the adhesive sheet 30 is bonded to the electrode unit 20 , And a cover sheet 40 is attached to the upper portion.

1, the biosensor strip 2 of the present invention is mounted on the measuring instrument 1 so that the connection portion 23 of the copper electrode layer 22 is inserted, and then the body fluid is injected through the injection groove 50 , The analytical value is measured in the measuring instrument 1.

The biosensor strip according to the present invention described above can increase the reliability of measured values by depositing nickel and forming copper and gold as an electrode layer.

The description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features presented herein.

1: Meter
2: Biosensor strip
10: base sheet 20: electrode part
21: first coating layer 22: copper electrode layer
23: connection part 24: reaction part
25: second coating layer 26: gold electrode layer
27: Reactor 30: Adhesive sheet
40: cover sheet 50: injection groove

Claims (6)

A biosensor strip comprising a base sheet of an insulating material and an electrode section formed on the base sheet, the biosensor strip being diagnosed through a body fluid flowing into the electrode section,
The electrode portion includes a first coating layer formed on the base sheet;
A copper electrode layer formed on the first coating layer;
A second coating layer formed on the first electrode layer and coated on the sample reaction unit for reaction with body fluids;
And a gold electrode layer formed on the second coating layer. The method according to claim 1,
Wherein the first coating layer and the second coating layer are formed by coating nickel to enhance adhesion between the copper electrode layer and the gold electrode layer. The method according to claim 1,
Wherein the first coating layer and the second coating layer are formed by depositing a copper electrode layer and a gold electrode layer. The method according to claim 1,
Wherein the copper electrode layer comprises a reference electrode, a working electrode, and a counter electrode. Coating a first coating layer on a base sheet of insulating material;
Depositing copper on the upper surface of the first coating layer to form a copper electrode layer having a connection part connected to the measurement and inserted into the measuring device at one side thereof and a reaction part through which a fresh sample is introduced at the other side;
Coating a second coating layer on the upper surface of the reaction part of the copper electrode layer;
And depositing gold on the upper surface of the second coating layer to form a gold electrode layer. 6. The method of claim 5,
Wherein the first coating layer and the second coating layer are formed by depositing nickel to increase adhesion between the copper electrode layer and the gold electrode layer.
KR1020150131338A 2015-09-17 2015-09-17 Biosensor strip and manufacturing method of it KR20150112911A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
KR1020150131338A KR20150112911A (en) 2015-09-17 2015-09-17 Biosensor strip and manufacturing method of it
PCT/KR2015/010380 WO2017047856A1 (en) 2015-09-17 2015-10-01 Biosensor strip and method of manufacturing same

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020150131338A KR20150112911A (en) 2015-09-17 2015-09-17 Biosensor strip and manufacturing method of it

Publications (1)

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KR20150112911A true KR20150112911A (en) 2015-10-07

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WO (1) WO2017047856A1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20180026085A (en) * 2016-09-02 2018-03-12 (주)지엠에스바이오 Glucose measuring strip and manufacturing method of the same
KR20210029588A (en) 2019-09-06 2021-03-16 (주)인트인 Chamber for inspecting body-fluid and body-fluid inspecting apparatus using the same

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100805913B1 (en) * 2005-12-16 2008-02-21 후앙 엘리스 와이 Structure and manufacturing method of disposable electrochemical sensor strip
KR100887632B1 (en) * 2007-03-07 2009-03-10 장용상 Biosensor
KR101003077B1 (en) * 2008-10-16 2010-12-21 세종공업 주식회사 Electrochemical biosensor structure and measuring method using the same
KR20100084233A (en) * 2009-01-16 2010-07-26 주식회사 에스디 Electrochemical biosensor strip
KR20140066377A (en) * 2012-11-23 2014-06-02 삼성전자주식회사 Bio-sensor

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20180026085A (en) * 2016-09-02 2018-03-12 (주)지엠에스바이오 Glucose measuring strip and manufacturing method of the same
KR20210029588A (en) 2019-09-06 2021-03-16 (주)인트인 Chamber for inspecting body-fluid and body-fluid inspecting apparatus using the same

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WO2017047856A1 (en) 2017-03-23

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