CN112113919A

CN112113919A - Enzyme-labeling instrument

Info

Publication number: CN112113919A
Application number: CN202010889507.2A
Authority: CN
Inventors: 彭南均; 冯忠彩; 夏贵银
Original assignee: Feishi Medical Equipment Wuhan Co ltd
Current assignee: Feishi Medical Equipment Wuhan Co ltd
Priority date: 2020-08-28
Filing date: 2020-08-28
Publication date: 2020-12-22

Abstract

The invention provides an enzyme-labeling instrument, which aims to solve the technical problems of high price and high heat generation of halogen lamps in the prior art. The power supply comprises a shell, a power supply and a CPU, wherein a cavity is formed in the shell; the light path system comprises a light source assembly, a light filtering assembly, a multi-branch optical fiber and a light processing assembly, wherein the light source assembly comprises a light source and a control circuit, the light source is integrated by a plurality of LED lamps with different wavelengths, the control circuit comprises a digital switch and a plurality of constant current power supply driving group circuits, the digital switch is correspondingly and electrically connected with one LED lamp through one constant current power supply driving group circuit, the CPU at least comprises a detection instruction receiving module and a control signal sending module, and the control signal sending module is electrically connected with a control signal input end; the light filtering component, the multi-branch optical fiber and the light processing component are sequentially connected along the path of the light ray; a signal processing system which is connected to the CPU and which includes a photoelectric converter and a signal processor; and the display screen is arranged outside the shell and is electrically connected with the signal processor.

Description

Enzyme-labeling instrument

Technical Field

The invention relates to the technical field of chemical analysis, in particular to an enzyme-labeling instrument.

Background

The enzyme-linked immunosorbent assay instrument is a special instrument for enzyme-linked immunosorbent assay, also called as a microporous plate detector, is widely applied to clinical examination, biological research and food and agricultural science research, and meanwhile, the enzyme-linked immunosorbent assay instrument is widely applied to the reproductive health care field to promote the improvement of the reproductive health level. At present, the optical system of the microplate reader mostly adopts a halogen lamp or xenon lamp light source, and a monochromatic light generator is matched to analyze and test a sample to be tested, and the halogen lamp and the xenon lamp have one or more defects of high price, large power consumption, more generated heat and the like, so that the use cost of the microplate reader is increased, and the halogen lamp generating high temperature can influence other parts if the heat is not dissipated timely, and further the service life of the microplate reader is shortened.

Disclosure of Invention

The invention provides an enzyme-labeling instrument, which aims to solve the technical problems of high price, large power consumption and high heat generation of halogen lamps in the prior art.

The scheme for solving the technical problems is as follows: an enzyme-labeling instrument is characterized by comprising a shell, wherein a cavity is formed in the shell, and a power supply and a CPU are installed in the cavity;

an optical path system comprising a light source assembly, a filtering assembly, a multi-branch optical fiber, and a light processing assembly, the light source assembly comprising a light source and a control circuit, the light source is integrated by a plurality of LED lamps with different wavelengths, the control circuit comprises a digital switch and a plurality of constant current power supply driving group circuits, the digital switch is provided with a power supply input end, a plurality of power supply output ends and a control signal input end, the power supply input ends are electrically connected with the power supply, each power supply output end is correspondingly and electrically connected with one LED lamp through a constant current power supply driving group circuit, the CPU at least comprises a detection instruction receiving module and a control signal sending module, the control signal sending module is electrically connected with the control signal input end, the detection instruction receiving module is used for receiving a detection instruction set by a user; the light filtering component, the multi-branch optical fiber and the light processing component are sequentially connected along a light path;

the signal processing system is connected to the CPU and comprises a photoelectric converter and a signal processor, the photoelectric converter is connected with the optical processing assembly and used for converting optical signals into electric signals, and the signal processor is electrically connected with the photoelectric converter and used for processing the electric signals;

and the display screen is arranged outside the shell and is electrically connected with the signal processor.

The microplate reader provided by the invention controls the switch of each LED lamp independently through the matching of the CPU and the control circuit, and as the light source comprises a plurality of LED lamps with different wavelengths, certain determined wavelength can be generated when different LEDs emit light, the LEDs are cold light sources, and the number of the emitted light of the LEDs is controlled, the heat generated by the microplate reader can be effectively controlled, and the maintenance cost of the microplate reader can be effectively reduced because the LED lamps have longer service life than tungsten halogen lamps.

The foregoing description is only an overview of the technical solutions of the present invention, and in order to make the technical solutions of the present invention more clearly understood and to implement them in accordance with the contents of the description, the following detailed description is given with reference to the preferred embodiments of the present invention and the accompanying drawings. The detailed description of the present invention is given in detail by the following examples and the accompanying drawings.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the invention without limiting the invention. In the drawings:

FIG. 1 is a schematic structural diagram of a microplate reader provided in an embodiment of the present invention;

FIG. 2 is a schematic top view of the structure of FIG. 1;

FIG. 3 is a schematic view of the internal structure of FIG. 1;

FIG. 4 is a schematic diagram of the filter assembly of FIG. 3;

FIG. 5 is a schematic structural diagram of the filter wheel shown in FIG. 4;

FIG. 6 is a control circuit diagram of the light source of FIG. 4;

FIG. 7 is a circuit connection block diagram of a microplate reader according to an embodiment of the present invention;

fig. 8 is a schematic light path diagram of a microplate reader according to an embodiment of the present invention.

Detailed Description

The principles and features of this invention are described below in conjunction with the following drawings, which are set forth by way of illustration only and are not intended to limit the scope of the invention. The invention is described in more detail in the following paragraphs by way of example with reference to the accompanying drawings. Advantages and features of the present invention will become apparent from the following description and from the claims. It is to be noted that the drawings are in a very simplified form and are not to precise scale, which is merely for the purpose of facilitating and distinctly claiming the embodiments of the present invention.

As shown in fig. 1 to 8, the present invention provides a microplate reader, which comprises a housing 1, an optical path system 2, a signal processing system 3, a display screen 4 and a cooling fan 5.

The casing 1 comprises a bottom case 11 and a cover 12, the bottom case 11 and the cover 12 are detachably covered, the cooling fan 5 is installed on the side wall of the bottom case 11, a cavity is formed between the bottom case 11 and the cover 12, and the power supply 13 and the CPU14 are installed in the cavity.

Wherein, the optical system 2 includes a light source assembly 21, a filter assembly 22, a multi-branch optical fiber 23 and a light processing assembly 24, the light source assembly 21 includes a light source 211 and a control circuit 212, the light source 21 is integrated by a plurality of LED lamps 21a with different wavelengths, the control circuit 212 includes a digital switch 2121 and a plurality of constant current power supply driving group circuits 2122, the digital switch 2121 has a power supply input end a, a plurality of power supply output ends b and a control signal input end c, the power supply input end a is electrically connected with the power supply 13, each power supply output end b is correspondingly and electrically connected with one LED lamp 21a through one constant current power supply driving group circuit 2122, the CPU14 includes at least a detection instruction receiving module 141 and a control signal transmitting module 142, the control signal transmitting module 142 is electrically connected with the control signal input, for sending a control signal to the digital switch 2121, and the detection instruction receiving module 141 is configured to receive a detection instruction set by a user; the filter assembly 22, the multi-branch optical fiber 23 and the light management assembly 24 are connected in series along the light path.

The constant current supply driving group circuit 2122 comprises an input circuit 2122a and a voltage-stabilizing output circuit 2122b, the input circuit 2122a is connected between the power supply 13 and the corresponding LED lamp 21a, the voltage-stabilizing output circuit 2122b is connected between the digital switch 2121 and the corresponding LED lamp 21a, the input circuit 2122a comprises a resistor and a field-effect transistor, R and a field-effect transistor Q, the resistor R is connected in series with the field-effect transistor G, one port of the field-effect transistor G is grounded, and the other port of the field-effect transistor G is electrically connected with the corresponding LED lamp 21 a; the regulated output circuit 2122b includes at least two diodes D arranged in series.

Specifically, in this embodiment, the resistance value of the resistor R is 1K ohm; the model of the field effect transistor Q is IRF3710, and the electric field effect of the control input loop is utilized to control the current of the output loop; the diode D has a model IN 4007.

The digital switch 2121 is of a HCF4051 type, and has eight power supply output terminals and four control signal input terminals, specifically, the eight power supply output terminals are pins X0-X7 in sequence, taking pin X0 as an example, and are connected in series with a resistor R and a field-effect transistor Q, one port of the field-effect transistor Q is connected with an electrical input terminal of an LED lamp 221, and an electrical output terminal of the LED lamp 221 is connected with two diodes D; pin JP27-JP30 of digital switch 2121 is connected to control signal transmission module 42.

The signal processing system 3 is connected to the CPU14 and includes a photoelectric converter 31 and a signal processor 32, the photoelectric converter 31 is connected to the optical processing assembly 24 for converting an optical signal into an electrical signal, and the signal processor 32 is electrically connected to the photoelectric converter 31 for processing the electrical signal.

The display 4 is mounted outside the housing 1 and electrically connected to the signal processor 32, and specifically, the display 4 is mounted on the cover 11.

The light source assembly 21 further includes a lamp cover 213, a lamp cup 214 and a lamp holder 215, the lamp cup 214 is disposed in the lamp holder 215, the light source 211 is mounted at the bottom of the lamp cup 214 and is located on a side of the lamp cup 214 away from the lamp holder 215, and the lamp cover 213 covers the outside of the lamp cup 214.

The wavelengths of the LED lamps 21a with different wavelengths are 340nm, 450nm, 492nm and 620/630nm respectively.

The filtering assembly 22 comprises a filtering front cover 221, a filtering rear cover 222, a filtering wheel 223 and a rotary driving mechanism 224, wherein the filtering front cover 221 and the filtering rear cover 222 are detachably covered and form a filtering cavity 22a inside, an aperture stop 221a communicated with the filtering cavity 22a is formed on the filtering front cover 221, the light source 211 is installed on the filtering front cover 221, the filter wheel 223 comprises a wheel body 2231 and a plurality of optical filters 2232, the wheel body 2231 is rotatably mounted in the filter cavity 22a, the rotation axis L of the wheel body 2231 is located at one side of the aperture stop 221a, a plurality of filter holes 223a are formed in the wheel body 2231, the optical filters 2232 are detachably mounted in the filter holes 223a in a one-to-one correspondence manner, the distance from the center of each filter hole 223a to the rotation axis L is equal to the distance from the center of the aperture stop 221a to the rotation axis L, and the rotary driving mechanism 224 is used for driving the wheel body 2231 to rotate.

The light source 211 and the aperture stop 221a are correspondingly arranged, so that light emitted by the light source 211 can be guaranteed to reach the filtering cavity through the aperture stop 221a to the maximum extent, and since the distance from the center of each filtering hole 223a to the rotation axis L is equal to the distance from the center of the aperture stop 221a to the rotation axis L, when the filter wheel 223 rotates, the filter 2232 on the filter wheel can be over against the aperture stop 221a to achieve filtering.

In order to fix the position of the optical fiber, in this embodiment, the optical fiber fixing sleeve 231 is further included, the light exit hole 222a is formed in the filtering rear cover 222 corresponding to the aperture stop 221a, the optical fiber fixing sleeve 231 is mounted on the filtering rear cover 222 corresponding to the light exit hole 222a, the multi-branch optical fiber 23 is inserted into the optical fiber fixing sleeve 231, and the end portion of the multi-branch optical fiber 231 is disposed corresponding to the light exit hole 222 a.

Specifically, a set screw 232 is mounted on the optical fiber fixing sleeve 231, and one end of the set screw 232 passes through the optical fiber fixing sleeve 231 and abuts against the optical fiber 23.

To ensure uniform distribution of the filters 2232 on the filter wheel 223, the filter holes 223a are uniformly spaced along the axis of rotation L on the filter wheel 223.

Specifically, the rotation driving mechanism 224 is a stepping motor, the stepping motor is mounted on the light filtering rear cover 222, an output shaft 2241 of the stepping motor extends into the light filtering cavity 22a, and the wheel body 2231 is coaxially mounted on the output shaft 2241 of the stepping motor.

When the stepping motor is installed, it is preset that each rotation angle is a determined angle, for example, when the number of the filter holes 223a is 8, it is set that each rotation angle is 45 °, in practical use, in order to ensure more accurate positioning of the rotation angle of the filter wheel 223, a notch may be formed in the edge of the filter wheel 223, a photoelectric sensor 2233 is arranged on the filter rear cover 222, the photoelectric sensor 5 uses the notch as a rotation starting point, and after each rotation, the photoelectric sensor 2233 determines the rotation angle of the filter wheel 223 based on the corresponding position of the notch, so as to determine accurate selection of the filter 2232.

In this embodiment, the installation manner of the filter 2232 is specifically as follows: the filter wheel 223 has a groove 223b formed at a position corresponding to the edge of the filter hole 223a, the filter 2232 is detachably pressed in the corresponding groove 223b by a pressing ring 2234, and the pressing ring 2234 is an elastic structure.

The press ring 2234 can be arranged to effectively facilitate the installation of the filter 2232 and the wheel 2231, and when the filter 2232 needs to be removed, the press ring 2234 only needs to be removed, and then the filter 2232 is poured out of the groove.

In this embodiment, the light processing assembly 24 includes a collimating mirror 241, a collecting mirror 243 and a sample gap 242, the collimating mirror 241, the sample gap 242 and the collecting mirror 243 are sequentially disposed at one end of the multi-branch optical fiber 23 away from the filter assembly 22, and the sample gap 242 is used for passing through the microplate 200.

In actual use, the microplate may be pre-positioned on a conveyor that transports the microplate from the cuvette to the location of the sample gap 242.

When the embodiment is used, firstly, the detection instruction receiving module 141 receives the detection instruction receiving module 141 set by a user, the control signal sending module 142 inputs a control signal to the control signal input end c of the digital switch 2121, and the digital switch 2121 determines that a certain specific constant current power supply driving group circuit 2122 is powered on according to the control signal, so that the LED lamp with a single wavelength emits light;

light emitted by the LED lamp passes through the optical filter 2232 and is called a purer monochromatic light beam; the light beam is transmitted to the collimating mirror 241 for collimation through the multi-branch optical fiber 23, the collimated light beam vertically passes through a liquid to be detected in a micropore of the elisa plate 200 from the bottom of the micropore of the elisa plate 200, and is converged on the photoelectric converter 31 through the condenser 243 to convert an optical signal into an electric signal, and the electric signal is processed by analog signals such as pre-amplification, logarithmic amplification, analog-to-digital conversion and the like by the signal processor 32 and then is sent to the display screen 4 for man-machine interaction.

The foregoing is merely a preferred embodiment of the invention and is not intended to limit the invention in any manner; those skilled in the art can readily practice the invention as shown and described in the drawings and detailed description herein; however, those skilled in the art should appreciate that they can readily use the disclosed conception and specific embodiments as a basis for designing or modifying other structures for carrying out the same purposes of the present invention without departing from the scope of the invention as defined by the appended claims; meanwhile, any changes, modifications, and evolutions of the equivalent changes of the above embodiments according to the actual techniques of the present invention are still within the protection scope of the technical solution of the present invention.

Claims

1. An enzyme mark instrument is characterized by comprising

The power supply comprises a shell, a power supply and a CPU, wherein a cavity is formed in the shell;

2. The microplate reader of claim 1, wherein the light source assembly further comprises a front cover, a lamp cup and a lamp holder, the lamp cup is arranged in the lamp holder, the light source is mounted at the bottom of the lamp cup and located on one side of the lamp cup away from the lamp holder, and the front cover is arranged outside the lamp cup.

3. The microplate reader of claim 1, wherein the wavelengths of the LED lamps with different wavelengths are 340nm, 450nm, 492nm, 620/630nm, respectively.

4. The microplate reader of claim 1, wherein the constant current power supply driving group circuit comprises an input circuit and a voltage stabilization output circuit, the input circuit is connected between the power supply and the corresponding LED lamp, the voltage stabilization output circuit is connected between the digital switch and the corresponding LED lamp, the input circuit comprises a resistor and a field effect transistor, the resistor is connected in series with the field effect transistor, one port of the field effect transistor is grounded, and the other port of the field effect transistor is electrically connected with the corresponding LED lamp; the voltage stabilizing output circuit comprises at least two diodes which are arranged in series.

5. The microplate reader of claim 1, wherein the filtering assembly comprises a filtering front cover, a filtering rear cover, a filtering wheel and a rotary driving mechanism, the filtering front cover and the filtering rear cover are detachably covered and form a filtering cavity inside, an aperture diaphragm communicated with the filtering cavity is formed on the filtering front cover, the light source is mounted on the filtering front cover and corresponds to the aperture diaphragm, the filtering wheel comprises a wheel body and a plurality of optical filters, the wheel body is rotatably mounted in the filtering cavity, the rotation axis of the wheel body is located on one side of the aperture diaphragm, a plurality of filtering holes are formed on the wheel body, the optical filters are detachably mounted in the filtering holes in a one-to-one correspondence manner, the distance from the center of each filtering hole to the rotation axis is equal to the distance from the center of the aperture diaphragm to the rotation axis, the rotary driving mechanism is used for driving the wheel body to rotate.

6. The microplate reader of claim 5, wherein the rotary drive mechanism is a stepper motor, the stepper motor is mounted on the light filtering rear cover, an output shaft of the stepper motor extends into the light filtering cavity, and the wheel body is coaxially mounted on the output shaft of the stepper motor.

7. The microplate reader of claim 5, further comprising an optical fiber fixing sleeve, wherein a light exit hole is formed in the filtering rear cover corresponding to the aperture stop, the optical fiber fixing sleeve is mounted on the filtering rear cover corresponding to the light exit hole, the multi-branch optical fiber is inserted into the optical fiber fixing sleeve, and an end portion of the multi-branch optical fiber is disposed corresponding to the light exit hole.

8. The microplate reader of claim 5, wherein the filter holes are uniformly distributed at intervals along the rotation axis in the filter wheel, grooves are formed in positions of the filter wheel corresponding to edges of the filter holes, the optical filter is detachably pressed in the corresponding grooves through a pressing ring, and the pressing ring is of an elastic structure.

9. The microplate reader of claim 1, wherein the light processing assembly comprises a collimating lens, a collecting lens and a sample gap, the collimating lens, the sample gap and the collecting lens are sequentially arranged at one end of the multi-branch optical fiber far away from the filtering assembly, and the sample gap is used for passing through an microplate.

10. The microplate reader of claim 1, further comprising a heat dissipation fan, wherein the housing comprises a bottom shell and a cover, the bottom shell and the cover are detachably covered, and the heat dissipation fan is mounted on a side wall of the bottom shell.