CN112111386A

CN112111386A - Bacterial colony picking instrument

Info

Publication number: CN112111386A
Application number: CN202010910616.8A
Authority: CN
Inventors: 何凯; 黄龙龙; 朱伟; 赵文亮
Original assignee: Shenzhen Institute of Advanced Technology of CAS
Current assignee: Shenzhen Institute of Advanced Technology of CAS
Priority date: 2020-09-02
Filing date: 2020-09-02
Publication date: 2020-12-22

Abstract

The application provides a bacterial colony picking instrument which comprises a bottom plate, a rotary disc, a plurality of groups of picking needle devices and a plurality of groups of bacterial colony picking devices, wherein the rotary disc is rotatably arranged on the bottom plate; the colony picking device comprises a culture dish moving mechanism, an inoculation pore plate moving mechanism and a disinfection and sterilization mechanism, wherein the culture dish moving mechanism, the inoculation pore plate moving mechanism and the disinfection and sterilization mechanism are sequentially distributed along the rotation direction of the turntable; and the bottom plate is also provided with an image acquisition mechanism for acquiring images of the bacterial colonies in the culture dish. The work of picking, inoculation, sterilization can be many times rotated a week to carousel in this application, has improved the work efficiency of picking the inoculation.

Description

Bacterial colony picking instrument

Technical Field

The application belongs to the technical field of biomedical instruments, and more particularly relates to a bacterial colony picking instrument.

Background

Biotechnology has found application in many areas, whether breeding strains directly from nature, or mutagenesis or genetic engineering, involving clonal colony picking. The traditional manual colony picking method cannot meet the requirement of high-flux clone screening, and compared with manual colony picking, the automatic colony picking instrument not only greatly improves the picking efficiency, but also greatly improves the picking precision. The common automatic colony picking instrument adopts a structure that a rotary disc drives a picking needle to rotate to circularly execute the repeated working steps of picking, inoculating and disinfecting, but the picking needle only executes the operation of picking, inoculating and disinfecting once when the rotary disc of the colony picking instrument rotates for one circle, and the picking and inoculating working efficiency is lower.

Disclosure of Invention

An object of the embodiment of this application is to provide an appearance is chosen to bacterial colony to solve the bacterial colony and choose the lower technical problem of inoculation work efficiency of choosing of appearance.

In order to achieve the purpose, the technical scheme adopted by the application is as follows: a bacterial colony picking instrument comprises a bottom plate, a rotary disc rotatably mounted on the bottom plate, a plurality of groups of picking needle devices arranged on the rotary disc at intervals along the circumferential direction of the rotary disc, and a plurality of groups of bacterial colony picking devices distributed along the rotating direction of the rotary disc in sequence; the colony picking device comprises a culture dish moving mechanism, an inoculation pore plate moving mechanism and a disinfection and sterilization mechanism, wherein the culture dish moving mechanism, the inoculation pore plate moving mechanism and the disinfection and sterilization mechanism are sequentially distributed along the rotating direction of the rotating disc, the culture dish moving mechanism is installed on the bottom plate and used for driving the culture dish to move, the inoculation pore plate moving mechanism is installed on the bottom plate and used for driving the inoculation pore plate to move, and the disinfection and sterilization mechanism is installed on the bottom plate and used for disinfecting and sterilizing the picking needle device; and the bottom plate is also provided with an image acquisition mechanism for acquiring images of the bacterial colonies in the culture dish.

Optionally, the culture dish moving mechanism includes picks Y axis nature module, picks X axis nature module and culture dish support bracket, it installs to pick Y axis nature module on the bottom plate and arrange along the Y axle direction, it installs to pick X axis nature module pick on the output of Y axis nature module and arrange along the X axle direction, culture dish support bracket is fixed in pick on the output of X axis nature module and be used for placing the culture dish.

Optionally, the inoculation orifice plate moving mechanism comprises an inoculation Y-axis linear module, an inoculation X-axis linear module and an inoculation orifice plate support bracket, the inoculation Y-axis linear module is mounted on the bottom plate and arranged along the Y-axis direction, the inoculation X-axis linear module is mounted on the output end of the inoculation Y-axis linear module and arranged along the X-axis direction, and the inoculation orifice plate support bracket is fixed on the output end of the inoculation X-axis linear module and used for placing the inoculation orifice plate.

Optionally, the picking Y-axis linear module, the picking X-axis linear module, the inoculating Y-axis linear module and the inoculating X-axis linear module are all provided with limit switches.

Optionally, the culture dish support bracket and the inoculation hole plate support bracket both comprise a support plate and a plurality of limiting blocks arranged on the support plate.

Optionally, the image capturing mechanism includes a camera mount, an industrial camera, and a backlight, the camera mount being mounted on the base plate, the industrial camera being mounted on the camera mount, and the backlight being mounted on the base plate and located directly below the industrial camera.

Optionally, the sterilization mechanism comprises a lifting bracket and a heating furnace, the lifting bracket is mounted on the bottom plate, and the heating furnace is mounted on the lifting bracket.

Optionally, the picking needle device comprises a picking needle and a driving cylinder, a cylinder body of the driving cylinder is mounted on the turntable, and the picking needle is mounted on a piston rod of the driving cylinder.

Optionally, the picking needle comprises a needle head and a connecting part which are connected with each other, and the connecting part is provided with a jack for inserting a piston rod of the driving cylinder.

Optionally, a magnetic sensor is arranged on the cylinder body of the driving cylinder, and a magnetic ring is sleeved on the piston rod of the driving cylinder.

Optionally, a rotating cylinder is connected to the bottom plate, the rotating cylinder includes a stator end and a rotor end that are rotatably connected to each other, the stator end of the rotating cylinder is fixedly connected to the bottom plate, the rotor end of the rotating cylinder is fixedly connected to the center of the turntable, and both the stator end and the rotor section of the rotating cylinder are provided with air holes through which air flows.

Optionally, the drum further comprises a vertical plate and a top plate, the vertical plate is vertically installed on the bottom plate, the top plate is horizontally connected to the upper end of the vertical plate, and the stator end of the drum is fixedly installed on the lower surface of the top plate.

Optionally, the turntable further comprises a base and a servo motor, wherein the base is fixedly installed on the bottom plate, the servo motor is installed on the base, and the output end of the servo motor is fixedly connected to the center of the turntable.

The embodiment of this application has following beneficial effect at least, through the rotation direction distribution multiunit bacterial colony along the carousel device of choosing, every group bacterial colony is chosen the device and is set up culture dish moving mechanism, inoculation orifice plate moving mechanism and the disinfection and isolation mechanism that distributes along the rotation direction sequence of carousel, distributes a plurality of groups on the carousel and chooses the needle device for every rotation a week of carousel, the bacterial colony is chosen the appearance and can carry out the work of a lot of "choosing, inoculation, sterilization" altogether. According to the calculation of the two groups of colony picking devices and the eight groups of picking needle devices, each group of picking needle devices can complete the operations of picking, idle station staying, inoculating, sterilizing, picking, idle station staying, inoculating and sterilizing when the turntable rotates for one circle, namely the work of picking, inoculating and sterilizing twice is completed; the eight groups of needle picking devices can complete sixteen times of picking, inoculating and sterilizing once the turntable rotates for one circle, so that the working efficiency of picking and inoculating is greatly improved.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the embodiments or the prior art descriptions will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without inventive exercise.

FIG. 1 is a schematic structural diagram of an embodiment of the present application;

FIG. 2 is a schematic structural diagram of another perspective in accordance with an embodiment of the present disclosure;

FIG. 3 is a top view of an embodiment of the present application;

FIG. 4 is a schematic structural diagram of an image acquisition mechanism and a culture dish moving mechanism in an embodiment of the present application;

FIG. 5 is a schematic view showing the assembly of the picking needle device in the embodiment of the present application;

FIG. 6 is a schematic view of the assembly of the turntable and the drum in the embodiment of the present application.

Wherein, each mark in the figure is:

11. a base plate; 12. a vertical plate; 13. a top plate; 14. a base; 2. a turntable; 21. an adapter; 3. a needle picking device; 31. picking a needle; 311. a needle head; 312. a connecting portion; 313. a jack; 32. a driving cylinder; 321. a cylinder body; 322. a piston rod; 33. a magnetic sensor; 34. a magnetic ring; 4. a bacterial colony picking device; 41. an image acquisition mechanism; 411. a camera support; 412. an industrial camera; 413. a backlight; 42. a culture dish moving mechanism; 421. picking a Y-axis linear module; 422. picking an X-axis linear module; 423. a culture dish support bracket; 43. an inoculation orifice plate moving mechanism; 431. inoculating the Y-axis linear module; 432. inoculating the X-axis linear module; 433. an inoculation well plate support bracket; 44. a sterilizing mechanism; 441. a liftable bracket; 442. heating the furnace; 45. a limit switch; 461. a support plate; 462. a limiting block; 5. a rotating drum; 51. a stator terminal; 52. a rotor end; 53. air holes; 6. a servo motor.

Detailed Description

In order to make the technical problems, technical solutions and advantageous effects to be solved by the present application clearer, the present application is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present application and are not intended to limit the present application.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly or indirectly connected to the other element. The terms "upper", "lower", "left", "right", and the like, indicate orientations or positional relationships based on those shown in the drawings, are merely for convenience of description, and do not indicate or imply that the referred devices or elements must have a specific orientation, be constructed in a specific orientation, and operate, and thus are not to be construed as limiting the patent, and the specific meanings of the above terms will be understood by those skilled in the art according to specific situations. The terms "first", "second" and "first" are used merely for descriptive purposes and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features. The meaning of "plurality" is two or more unless specifically limited otherwise.

As shown in fig. 1 to 3, an embodiment of the present application provides a bacterial colony picking apparatus, which includes a bottom plate 11, a rotating disc 2, a plurality of groups of picking needle devices 3, and a plurality of groups of bacterial colony picking devices 4; wherein, carousel 2 rotates to be installed on bottom plate 11, and a plurality of groups pick needle device 3 along 2 circumference intervals arrangements on carousel 2 of carousel, and the rotation direction order along carousel 2 is picked to the multiunit bacterial colony and is put 4.

Each group of colony picking devices 4 comprises a culture dish moving mechanism 42, an inoculation pore plate moving mechanism 43 and a disinfection and sterilization mechanism 44, and the culture dish moving mechanism 42, the inoculation pore plate moving mechanism 43 and the disinfection and sterilization mechanism 44 in each group of colony picking devices 4 are sequentially distributed along the rotation direction of the turntable 2; the culture dish moving mechanism 42 is installed on the bottom plate 11 and is used for driving the culture dish to perform plane motion, the inoculation hole plate moving mechanism 43 is installed on the bottom plate 11 and is used for driving the inoculation hole plate to perform plane motion, and the disinfection and sterilization mechanism 44 is installed on the bottom plate 11 and is used for disinfecting and sterilizing the picking needle device 3. The bottom plate 11 is also provided with an image acquisition mechanism 41, the image acquisition mechanism 41 is used for acquiring the image of the bacterial colony in the culture dish,

because the colony picking device 4 is provided with a plurality of groups, and each group of colony picking device 4 comprises a culture dish moving mechanism 42, an inoculation pore plate moving mechanism 43 and a disinfection and sterilization mechanism 44, at least two sets of culture dish moving mechanisms 42, at least two sets of inoculation pore plate moving mechanisms 43 and at least two sets of disinfection and sterilization mechanisms 44 are arranged in the whole colony picking instrument, and are sequentially distributed along the rotation direction of the turntable 2 according to the sequence of the culture dish moving mechanisms 42, the inoculation pore plate moving mechanisms 43, the disinfection and sterilization mechanisms 44, the culture dish moving mechanisms 42, the inoculation pore plate moving mechanisms 43 and the disinfection and sterilization mechanisms 44 … ….

Taking two groups of colony picking devices 4 and eight groups of picking needle devices 3 as examples, the whole colony picking instrument is provided with two sets of culture dish moving mechanisms 42, two sets of inoculation pore plate moving mechanisms 43 and two sets of disinfection and sterilization mechanisms 44. Two sets of image acquisition mechanisms 41 are symmetrically arranged on the bottom plate 11, two sets of culture dish moving mechanisms 42 are symmetrically arranged on the bottom plate 11, two sets of inoculation pore plate moving mechanisms 43 are symmetrically arranged on the bottom plate 11, and two sets of disinfection and sterilization mechanisms 44 are symmetrically arranged on the bottom plate 11.

During operation, the two sets of culture dish moving mechanisms 42 located at the front left and the back right of fig. 1 respectively move the corresponding culture dish to the positions of the two sets of image acquisition mechanisms 41, the two sets of image acquisition mechanisms 41 respectively acquire images of bacterial colonies in the corresponding culture dish, and image coordinates representing the positions of the bacterial colonies are converted into world coordinates in a computer and are subjected to path planning, which may be referred to as image acquisition for short.

Then, the two sets of culture dish moving mechanisms 42 respectively move the first bacterial colony in the planned path in the corresponding culture dish to the position under the corresponding picking needle device 3 installed on the turntable 2, the two sets of picking needle devices 3 respectively move downwards and pick the bacterial colony in the corresponding culture dish, and after the completion, the two sets of picking needle devices 3 respectively move upwards to the original position, which may be referred to as picking for short.

Then the turntable 2 rotates a certain angle, so that the two groups of picking needle devices 3 respectively picking the bacterial colonies rotate to the positions right above the corresponding inoculation pore plate moving mechanisms 43, then the two groups of picking needle devices 3 respectively picking the bacterial colonies move downwards and are inserted into the culture solution in the corresponding inoculation pore plates, so that the bacterial colonies picked by the picking needle devices 3 fall into the culture solution, and after the completion, the two groups of picking needle devices 3 respectively move upwards to the original positions, which can be referred to as inoculation for short.

Then the turntable 2 continues to rotate for a certain angle, so that the two groups of picking needle devices 3 respectively rotate to the positions right above the corresponding disinfection and sterilization mechanisms 44, the inoculated picking needle devices 3 move downwards and are inserted into the corresponding disinfection and sterilization mechanisms 44, the disinfection and sterilization mechanisms 44 disinfect and sterilize the residual bacterial colonies on the picking needle devices 3, preparation is made for the next picking step of the picking needle devices 3, after the completion, the two groups of picking needle devices 3 respectively move upwards to the original positions, and the step can be referred to as sterilization for short.

This application embodiment picks device 4 through the direction of rotation that follows carousel 2 multiunit bacterial colony that distributes in proper order, and every group bacterial colony picks device 4 and sets up culture dish moving mechanism 42, inoculation orifice plate moving mechanism 43 and the disinfection and isolation mechanism 44 that distributes along the direction of rotation sequence of carousel 2, distributes a plurality of groups on the carousel 2 and picks needle device 3 for every rotation a week of carousel 2, the bacterial colony is picked the appearance and can carry out the work of "picking, inoculation, sterilization" many times altogether. According to the calculation of the two groups of colony picking devices 4 and the eight groups of picking needle devices 3, when the turntable 2 rotates for a certain angle, the two groups of picking needle devices 3 can respectively complete picking, the two groups of picking needle devices 3 can respectively complete inoculation, the two groups of picking needle devices 3 can respectively complete sterilization, and the other two groups of picking needle devices 3 are idle; each group of the picking needle devices 3 can complete the operations of picking, idle station staying, inoculating, sterilizing, picking, idle station staying, inoculating and sterilizing after the turntable 2 rotates for one circle, namely, the operations of picking, inoculating and sterilizing are completed twice; every time the rotary table 2 rotates for a circle, the eight groups of the picking needle devices 3 can complete sixteen times of picking, inoculating and sterilizing, so that compared with the existing colony picking instrument, the working efficiency of picking and inoculating is greatly improved, the picking precision is improved, the time of each step is short, the inoculating survival rate is further improved, and the device has the advantages of simple integral structure, small space occupancy rate and the like.

In one embodiment, the dish moving mechanism 42 includes a picking Y-axis linear module 421, a picking X-axis linear module 422 and a dish support bracket 423, the picking Y-axis linear module 421 is installed on the bottom plate 11 and arranged along the Y-axis direction, the picking X-axis linear module 422 is installed on the output end of the picking Y-axis linear module 421 and arranged along the X-axis direction, and the dish support bracket 423 is fixed on the output end of the picking X-axis linear module 422 and used for placing a dish. The picking Y-axis linear module 421 can drive the picking X-axis linear module 422 to move in the Y-axis direction, and the picking X-axis linear module 422 can drive the culture dish support bracket 423 to move in the X-axis direction, so that the culture dish can perform plane motion on a plane and reach the position of the image acquisition mechanism 41 and under the picking needle device 3, and the structure is simple and the installation is convenient.

In one embodiment, the inoculation well plate moving mechanism 43 comprises an inoculation Y-axis linear module 431, an inoculation X-axis linear module 432 and an inoculation well plate support bracket 433, the inoculation Y-axis linear module 431 is mounted on the bottom plate 11 and arranged along the Y-axis direction, the inoculation X-axis linear module 432 is mounted on the output end of the inoculation Y-axis linear module 431 and arranged along the X-axis direction, and the inoculation well plate support bracket 433 is fixed on the output end of the inoculation X-axis linear module 432 and used for placing an inoculation well plate. The inoculation Y-axis linear module 431 can drive the inoculation X-axis linear module 432 to move in the Y-axis direction, and the inoculation X-axis linear module 432 can drive the inoculation orifice plate support bracket 433 to move in the X-axis direction, so that the inoculation orifice plate can move on the plane to reach the position right below the picking needle device 3, and the structure is simple and the installation is convenient.

In one embodiment, the pick Y axis linear module 421, the pick X axis linear module 422, the inoculation Y axis linear module 431, and the inoculation X axis linear module 432 are all mounted with limit switches 45. Taking the picking Y-axis linear module 421 and the picking X-axis linear module 422 as an example, the two ends of the picking Y-axis linear module 421 are respectively installed with a limit switch 45, when the picking X-axis linear module 422 moves to the end position of the picking Y-axis linear module 421, the limit switch 45 at the end of the picking Y-axis linear module 421 is triggered, so that the picking Y-axis linear module 421 stops driving the picking X-axis linear module 422 to move; the two ends of the picking X-axis linear module 422 are respectively provided with a limit switch 45, when the culture dish support bracket 423 moves to the end position of the picking X-axis linear module 422, the limit switch 45 at the end of the picking X-axis linear module 422 is triggered, so that the picking X-axis linear module 422 stops driving the culture dish support bracket 423 to move. By installing the limit switches 45 on the picking Y-axis linear module 421, the picking X-axis linear module 422, the inoculation Y-axis linear module 431 and the inoculation X-axis linear module 432 respectively, each linear module can be stopped in time, and the culture dish moving mechanism 42 and the inoculation hole plate moving mechanism 43 can be ensured to work normally.

In one embodiment, as shown in FIGS. 1 and 4, the culture dish support bracket 423 and the inoculation well plate support bracket 433 each include a support plate 461 and a plurality of stop blocks 462 disposed on the support plate 461. When the culture dish and the inoculation orifice plate are respectively placed on the corresponding bearing plate 461, the plurality of limiting blocks 462 on the corresponding bearing plate 461 respectively limit the culture dish and the inoculation orifice plate, so that the culture dish and the inoculation orifice plate can be respectively fixed and are not easy to deviate, and the structure is more stable.

In one embodiment, the image capturing mechanism 41 includes a camera holder 411, an industrial camera 412, and a backlight 413, the camera holder 411 being mounted on the base plate 11, the industrial camera 412 being mounted on the camera holder 411, and the backlight 413 being mounted on the base plate 11 and being directly below the industrial camera 412. The culture dish moving mechanism 42 moves the culture dish to a position between the backlight lamp 413 and the industrial camera 412, so that the backlight lamp 413 is positioned under the culture dish, the camera of the industrial camera 412 is positioned over the culture dish, and an image of a bacterial colony in the culture dish is acquired.

In one embodiment, as shown in fig. 1 and 2, the sterilization mechanism 44 includes a liftable support 441 and a heating melter 442, the liftable support 441 is mounted on the base plate 11, and the heating melter 442 is mounted on the liftable support 441. The picking needle device 3 is sterilized by inserting the picking needle device 3 into the heating furnace 442 and heating to a high temperature, so that the picking needle device 3 is clean and sanitary when the picking step is performed next time; further, the stroke of each downward movement of the picking needle device 3 is generally fixed, and the heating furnace 442 is mounted on the liftable holder 441 so as to be able to ascend and descend, whereby the height of the heating furnace 442 and hence the depth of insertion of the picking needle device 3 into the heating furnace 442 can be adjusted, and in the case of the same time, the larger the depth of insertion, the higher the temperature to which the picking needle device 3 is heated, and hence the temperature to which the picking needle device 3 is heated can be adjusted.

In one embodiment, as shown in fig. 1 and 5, the picking needle device 3 comprises a picking needle 31 and a driving air cylinder 32, a cylinder 321 of the driving air cylinder 32 is mounted on the turntable 2, and the picking needle 31 is mounted on a piston rod 322 of the driving air cylinder 32. The picking needle 31 is driven to move downwards and upwards by the driving air cylinder 32, so that the picking needle 31 can quickly complete the steps of picking and inoculating, the structure is simple, the action is sensitive, and the survival rate of bacterial colonies is further improved.

In one embodiment, the picking needle 31 includes a needle 311 and a connecting part 312 connected to each other, and the connecting part 312 is opened with an insertion hole 313 into which a piston rod 322 of the driving cylinder 32 is inserted. The picking needle 31 is fixedly arranged on the piston rod 322 of the driving cylinder 32 through the insertion hole 313 of the connecting part 312 and the insertion and matching of the piston rod 322 of the driving cylinder 32, and the structure is simple and the installation is convenient.

In one embodiment, the cylinder 321 of the driving cylinder 32 is provided with a magnetic sensor 33, and the piston rod 322 of the driving cylinder 32 is sleeved with a magnetic ring 34. When the driving cylinder 32 drives the picking needle 31 to move up to the home position, if the signal sent by the magnetic sensor 33 is not detected, it represents that the distance between the magnetic ring 34 and the magnetic sensor 33 exceeds the preset value, that is, it indicates that the piston rod 322 of the driving cylinder 32 is not normally retracted to the home position, so the turntable 2 does not continue to rotate to the next station. Through the design of the magnetic sensor 33 and the magnetic ring 34, the picking needle 31 can be ensured to move up to a predetermined position every time picking, inoculating and sterilizing, namely, after the magnetic sensor 33 detects that the magnetic ring 34 retracts, the turntable 2 can continue to rotate by a fixed angle, so that the picking needle 31 can be ensured to have higher colony picking precision under the condition that the steps of picking, inoculating and sterilizing are carried out repeatedly.

In one embodiment, as shown in fig. 1 and 6, the bottom plate 11 is connected to the rotating drum 5, the rotating drum 5 includes a stator end 51 and a rotor end 52 that are rotatably connected to each other, the stator end 51 of the rotating drum 5 is fixedly connected to the bottom plate 11, the rotor end 52 of the rotating drum 5 is fixedly connected to the center of the rotating disc 2, and both the stator end 51 and the rotor section of the rotating drum 5 are provided with air holes 53 through which air flows. When the turntable 2 rotates, the driving air cylinder 32 rotates together with the turntable 2, but the driving air cylinder 32 must be connected with an air pipe, so that the air pipes of the plurality of driving air cylinders 32 on the turntable 2 easily twine with each other during the rotation of the turntable 2. By arranging the rotary drum 5 with the stator end 51 and the rotor end 52 which are rotationally connected with each other, the air hole 53 of the stator end 51 and the air hole 53 of the rotor end 52 are respectively used for connecting air pipes, so that air flow enters the rotary drum 5 from the air hole 53 of the stator end 51 of the rotary drum 5 and then enters the driving air cylinder 32 from the air hole 53 of the rotor end 52 of the rotary drum 5, the air flow can be conveyed to the driving air cylinder 32 through the inner space of the rotary drum 5, and the air pipes connected between the driving air cylinder 32 and the air hole 53 of the rotor end 52 rotate along with the rotary disc 2 in the rotation process of the rotary disc 2, so that the air pipes cannot be intertwined.

In one embodiment, the colony picking apparatus further comprises a vertical plate 12 and a top plate 13, the vertical plate 12 is vertically mounted on the bottom plate 11, the top plate 13 is horizontally connected to the upper end of the vertical plate 12, and the stator end 51 of the rotary drum 5 is fixedly mounted on the lower surface of the top plate 13. Through the structure that sets up riser 12 and roof 13, make the stator end 51 of rotary drum 5 can be located the center department of carousel 2 directly over, simple structure and simple to operate also can not influence the normal action of other structures in the appearance is picked to the bacterial colony simultaneously.

In one embodiment, the adaptor 21 is fixedly mounted at the center of the turntable 2, and the stator end 51 of the rotary drum 5 is fixedly connected to the adaptor 21, so that the structure is simple and the installation is convenient.

In one embodiment, as shown in fig. 1 and 2, the colony picking apparatus further includes a base 14 and a servo motor 6, the base 14 is fixedly installed on the bottom plate 11, the servo motor 6 is installed on the base 14, and an output end of the servo motor 6 is fixedly connected to the center of the turntable 2. Drive carousel 2 through servo motor 6 and rotate, can improve the position accuracy that carousel 2 rotated and stopped, make carousel 2 rotate the stopping that can both be timely accurate after certain angle at every turn, further improved the bacterial colony and picked the precision.

The above description is only exemplary of the present application and should not be taken as limiting the present application, as any modification, equivalent replacement, or improvement made within the spirit and principle of the present application should be included in the protection scope of the present application.

Claims

1. The bacterial colony picking instrument is characterized by comprising a bottom plate, a rotary disc, a plurality of groups of picking needle devices and a plurality of groups of bacterial colony picking devices, wherein the rotary disc is rotatably arranged on the bottom plate, the groups of picking needle devices are arranged on the rotary disc at intervals along the circumferential direction of the rotary disc, and the groups of bacterial colony picking devices are sequentially distributed along the rotating direction of the rotary disc; the colony picking device comprises a culture dish moving mechanism, an inoculation pore plate moving mechanism and a disinfection and sterilization mechanism, wherein the culture dish moving mechanism, the inoculation pore plate moving mechanism and the disinfection and sterilization mechanism are sequentially distributed along the rotating direction of the rotating disc, the culture dish moving mechanism is installed on the bottom plate and used for driving the culture dish to move, the inoculation pore plate moving mechanism is installed on the bottom plate and used for driving the inoculation pore plate to move, and the disinfection and sterilization mechanism is installed on the bottom plate and used for disinfecting and sterilizing the picking needle device; and the bottom plate is also provided with an image acquisition mechanism for acquiring images of the bacterial colonies in the culture dish.

2. The colony picking instrument according to claim 1, wherein the culture dish moving mechanism comprises a picking Y-axis linear module, a picking X-axis linear module and a culture dish support bracket, the picking Y-axis linear module is mounted on the bottom plate and arranged along a Y-axis direction, the picking X-axis linear module is mounted on an output end of the picking Y-axis linear module and arranged along an X-axis direction, and the culture dish support bracket is fixed on the output end of the picking X-axis linear module and used for placing the culture dish.

3. The colony picking apparatus according to claim 2, wherein the inoculation hole plate moving mechanism comprises an inoculation Y-axis linear module, an inoculation X-axis linear module and an inoculation hole plate support bracket, the inoculation Y-axis linear module is mounted on the bottom plate and arranged along the Y-axis direction, the inoculation X-axis linear module is mounted on the output end of the inoculation Y-axis linear module and arranged along the X-axis direction, and the inoculation hole plate support bracket is fixed on the output end of the inoculation X-axis linear module and used for placing the inoculation hole plate.

4. The colony picking instrument according to claim 3, wherein the picking Y-axis linear module, the picking X-axis linear module, the inoculation Y-axis linear module and the inoculation X-axis linear module are all provided with limit switches.

5. The colony picking instrument according to claim 3, wherein the culture dish support bracket and the inoculation hole plate support bracket each comprise a support plate and a plurality of limit blocks arranged on the support plate.

6. The colony picking instrument as claimed in claim 1, wherein the image capturing mechanism comprises a camera bracket mounted on the base plate, an industrial camera mounted on the camera bracket, and a backlight mounted on the base plate and directly below the industrial camera.

7. The colony picking instrument according to claim 1, wherein the sterilizing mechanism comprises a liftable support and a heating furnace, the liftable support is mounted on the bottom plate, and the heating furnace is mounted on the liftable support.

8. The colony picking instrument according to claim 1, wherein the picking needle device comprises a picking needle and a driving cylinder, the cylinder body of the driving cylinder is mounted on the turntable, and the picking needle is mounted on the piston rod of the driving cylinder.

9. The colony picking instrument according to claim 8, wherein the picking needle comprises a needle head and a connecting part which are connected with each other, and the connecting part is provided with a jack for inserting a piston rod of the driving cylinder.

10. The colony picking instrument according to claim 8, wherein a magnetic sensor is arranged on the cylinder body of the driving cylinder, and a magnetic ring is sleeved on the piston rod of the driving cylinder.

11. The colony picking instrument according to claim 8, wherein the bottom plate is connected with a rotating drum, the rotating drum comprises a stator end and a rotor end which are mutually rotatably connected, the stator end of the rotating drum is fixedly connected with the bottom plate, the rotor end of the rotating drum is fixedly connected with the center of the rotating disc, and the stator end and the rotor section of the rotating drum are both provided with air holes for air flow to pass through.

12. The colony picking instrument according to claim 11, further comprising a vertical plate vertically mounted on the bottom plate, and a top plate horizontally connected to an upper end of the vertical plate, wherein the stator end of the rotary drum is fixedly mounted on a lower surface of the top plate.

13. The colony picking instrument according to claim 1, further comprising a base and a servo motor, wherein the base is fixedly mounted on the bottom plate, the servo motor is mounted on the base, and an output end of the servo motor is fixedly connected to the center of the turntable.