CN112088894A - Efficient novel synergistic combination for preventing and treating brown planthopper of rice - Google Patents
Efficient novel synergistic combination for preventing and treating brown planthopper of rice Download PDFInfo
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- CN112088894A CN112088894A CN202011158374.8A CN202011158374A CN112088894A CN 112088894 A CN112088894 A CN 112088894A CN 202011158374 A CN202011158374 A CN 202011158374A CN 112088894 A CN112088894 A CN 112088894A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/38—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< where at least one nitrogen atom is part of a heterocyclic ring; Thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
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Abstract
The invention discloses an efficient novel synergistic combination for preventing and treating brown planthopper of rice, which comprises main effective components consisting of indoxacarb and trifluoropyrimidine. Wherein the mass ratio of the indoxacarb to the trifluoro-benzene pyrimidine in the main effective component is 5: 1. Compared with the prior art, the indoxacarb and the trifluoro-benzene pyrimidine are combined and mixed, the combination has obvious synergistic effect on the prevention and treatment of the brown planthopper of the rice, the field prevention and treatment effect is as high as 93.04 percent, the prevention and treatment effect is obvious, the using amount of pesticides can be effectively reduced, various diseases can be treated, the drug resistance of pests is delayed, and the production cost is saved. The invention is simple and easy to operate, has wide material source, low cost and good use effect.
Description
Technical Field
The invention relates to the technical field of agricultural science and technology, in particular to an efficient novel synergistic combination for preventing and treating brown planthopper of rice.
Background
Nilaparvata lugens (Nilaparvata lugens) for brown plant hopper
Is an important pest in rice production, has the characteristics of monophagia, migration, destructiveness and the like, and is very easy to outbreak and cause disasters. The excreted honeydew can promote the growth of pathogenic bacteria, and the oral needle can also be used as the transmission medium of plant virus. This results in a severe decrease in rice yield, even no grain harvest in rice areas, and a huge economic loss. In the comprehensive control of the brown planthopper, the chemical control plays a crucial role in the comprehensive control by virtue of the advantages of rapidness, high efficiency and economy, but the resistance problem is more serious along with the single large-dose application of chemical agents. In the face of the intractable resistance problem, the selection of target differential agents for combined application in the field becomes a powerful measure for delaying resistance. The control effect of the pesticide is closely related to the pesticide, crops, control targets and environmental conditions, and the synergistic effect generated by the combination of the pesticide can obtain better control effect than that of single application, improve the control effect of single application, delay the generation of pest resistance, expand the insecticidal spectrum and reduce the dosage of main drugs, so that the pesticide is used at the lowest dosageThe maximum effect can be achieved within the dosage. Therefore, finding some efficient synergistic combinations for preventing and treating the brown planthopper of the rice has very important significance for reducing the times of using the pesticide, the dosage, delaying the generation of the pesticide resistance and improving the prevention and treatment effect in the field.
In the prior art, the invention patent with the publication number of CN107278741A discloses a method for preventing and controlling brown planthopper by utilizing red-colored lygus lucorum, which comprises the following steps: step one, sampling brown planthoppers in the target rice field by using a five-point sampling method and identifying the insect ages of the brown planthoppers; step two; counting the population density of 2-3 instar brown planthoppers in the target rice field by using an investigation sampling method; step three, implementing prevention and control work: draining accumulated water in the target rice field, and then releasing 4-year nymphs and 5-year nymphs of the red color lygus lucorum in a single time, wherein the number ratio of the 4-year nymphs and the 5-year nymphs of the red color lygus lucorum to the 2-3-year brown planthopper is 2: 1.5-2: 60. However, the scheme needs to feed a large amount of red-colored lygus lucorum and is difficult to popularize in a large range. In addition, the invention patent of CN107549204A discloses a composite pesticide for rice composite brown planthopper, which comprises the following components in parts by weight: 3-9 parts of triflumuron, 4-6 parts of nitenpyram and pymetrozine, 14-30 parts of a plant source additive, 2-8 parts of dinotefuran, 5-10 parts of chlorpyrifos, 6-11 parts of an emulsifier, 7-12 parts of a solvent, 5-13 parts of a stabilizer and 7-16 parts of a carrier. The adopted medicinal components are conventional, and the medicament is easy to generate medicament resistance and difficult to obtain a durable prevention and treatment effect.
Disclosure of Invention
The purpose of the invention is: the efficient novel synergistic combination for preventing and treating the brown planthopper of the rice has the advantages of obvious difference of action targets, obvious and safe synergistic action, capability of prolonging the service life of pesticides, reduction of prevention and treatment times and the use amount of chemical pesticides and the like.
The invention is realized by the following steps:
the invention relates to an efficient novel synergistic combination for preventing and treating brown planthopper of rice, which comprises main effective components consisting of indoxacarb and trifluoropyrimidine. Wherein the mass ratio of the indoxacarb to the trifluoro-benzene pyrimidine in the main effective component is 5: 1.
According to the technical scheme, the indoxacarb (indoxacarb) adopted in the method can generate an activated metabolite in an insect body to block a sodium ion channel, so that target insects can not eat and normally move, and finally die. Trifluzopyrimidine (triflumzopyrim) is a novel mesoionic insecticide acting on the orthosteric binding site of nicotinic acetylcholine receptors. The invention selects two insecticides with different action mechanisms of indoxacarb and trifluoro-benzene pyrimidine, and the combination of the two insecticides can play roles in expanding the control range, reducing the application amount, delaying the resistance and the like.
Compared with the prior art, the indoxacarb and the trifluoro-benzene pyrimidine are combined and mixed, the combination has obvious synergistic effect on the prevention and treatment of the brown planthopper of the rice, the field prevention and treatment effect is as high as 93.04 percent, the prevention and treatment effect is obvious, the using amount of pesticides can be effectively reduced, various diseases can be treated, the drug resistance of pests is delayed, and the production cost is saved. The invention is simple and easy to operate, has wide material source, low cost and good use effect.
Drawings
FIG. 1 shows the effect of indoxacarb and triflumopyrimidine and combinations thereof on Nilaparvata lugens esterase;
FIG. 2 shows the effect of indoxacarb with triflumopyrimidine and combinations thereof on Nilaparvata lugens glutathione S-transferase.
Detailed Description
The technical solution of the present invention will be further explained with reference to the specific embodiments. The technical features mentioned in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other. The ingredients or materials involved in the following processes, unless otherwise specified, are commercially available. The related experimental methods are conventional methods in the technical field if not specifically stated. The numerical values or numerical proportions, if not indicated, are mass values or mass proportions.
Test example 1: in order to further verify the technical effect of the invention, the product of the invention is subjected to a condition test.
1.1 Experimental materials
1.1.1 sources of test insects
Brown planthopper population in 3D Guizhou (Huangping, Huihe, Meitan) field is adopted, Jinyou 785 rice seedlings are fed in a cage in an isolation mode until 3 rd generation nymphs in F1 or F2 generation are used for indoor toxicity measurement.
1.1.2 test Agents
90% of indoxacarb raw drug (Hubei Zhengxing-sourced fine chemical Co., Ltd.), 10% of trifluorophenylpyrimidine suspending agent (DuPont, U.S.A.) and 15% of indoxacarb suspending agent (Shandong Shenda crop science and technology Co., Ltd.).
1.2 Experimental methods
1.2.1 Rice seedling impregnation method
LC for determining two agents by rice seedling soaking method50And (4) carrying out combined virulence determination on different combinations of the indoxacarb and the trifluoropyrimidine, and screening out the optimal combination. In the experiment, the indoxacarb and the trifluoro-benzene pyrimidine are combined according to the effective mass ratio of 1: 1, 3: 1, 5: 1, 7: 1, 9: 1, 1: 3, 1: 5, 1: 7 and 1: 9 respectively, and each combination is diluted into liquid medicine with a series of concentration gradients for soaking seedlings. Taking the rice seedlings with roots which are cultured indoors and have the length of about 10cm, washing the roots until no excessive impurities exist, wherein 1 repeat is carried out on every 15 rice seedlings, and 3 repeats are carried out on one mass concentration. The method comprises the steps of placing cleaned rice seedlings in the shade, drying the rice seedlings until no water mark exists on the surfaces of the rice seedlings, soaking the rice seedlings for 30s respectively according to the sequence of the concentration of liquid medicine from low to high, taking out the rice seedlings and drying the liquid medicine on the surfaces of the rice seedlings by taking distilled water containing 0.5% Tween-20 as a reference, wrapping the rice seedlings below the stem bases of the rice seedlings by soaked absorbent cotton after the liquid medicine is dried in the air so that seeds cannot be exposed, and placing the wrapped rice seedlings into a cup added with 30-35 mL of nutrient solution to prevent the rice seedlings from withering in a test time and further causing unnecessary experimental errors. Sucking 3-year-old midterm nymphs of brown planthoppers with consistent physiology, carefully pouring the test insects into cups with rice seedlings, wherein 15 nymphs in each cup are 1 repetition, each concentration is 3 repetitions, the total number of the nymphs is 45, and after the midterm nymphs of the brown planthoppers are poured in a large number, the cup mouths are sealed by cotton gauze and rubber bands to prevent the test insects from climbing out. The culture cup is put into an incubator with the temperature of (28 +/-1) DEG C and the photoperiod L: D of 16: 8 for culture, and the death number of the test insects is recorded after 48 h. Calculating virulence regression equation and LC by DPS software50Correlation coefficient ofAnd LC5095% confidence interval.
1.2.2 Co-toxicity coefficient (CTC) value calculation
And (3) calculating a virulence index, a theoretical virulence index, an actual virulence index and a cotoxicity coefficient (CTC) value of the mixture by adopting a Sun Yunpei method, and performing comprehensive evaluation according to the virulence index, the theoretical virulence index, the actual virulence index and the cotoxicity coefficient (CTC) value, wherein the CTC value is more than 120 and is equal to or less than 80, and the CTC value is equal to or less than 80.
The formula for calculating the co-toxicity coefficient CTC value is as follows:
theoretical virulence index (TTI) of the combination (virulence index of agent a × Ax) + (virulence index of agent B × Bx)
In the formula, Ax is the percentage content of the medicament A in the mixture;
bx is the percentage of the medicament B in the mixture.
1.2.3 determination of Brown planthopper detoxification enzyme Activity with synergistic combinations
1.2.3.1 synergistic combination sublethal concentration treatment
Obtaining the toxicity regression equation of the combination of indoxacarb, trifluralopyrimidine and indoxacarb-trifluralopyrimidine (5: 1) through indoor toxicity measurement, and respectively calculating LC25And (3) treating the indoor population of the brown planthopper with the obtained sub-lethal concentration, wherein the treatment method is the same as the virulence determination method, 200-300 nymphs of the brown planthopper at the age of 3 are treated each time, and the live insects are taken to prepare an enzyme solution after 48 hours of treatment.
1.2.3.2 carboxylesterase (CarE) Activity assay
2mL centrifuge tube was filled with 1mL of 3X 10-4Preheating mol/L alpha-naphthyl acetate substrate solution in a water bath at 37 ℃ for 3min, adding 0.2mL of esterase determination solution, shaking uniformly, reacting in the water bath at 37 ℃ for 15min, adding 0.2mL of color developing agent to terminate the reaction and develop color, and measuring the light absorption at 600nm in a colorimetric way after stabilizing for 30minValues, heat inactivated enzyme source as control. The amount of alpha-naphthol generated per ml of enzyme solution is calculated according to the standard curve of alpha-naphthol, the protein content of the enzyme solution is obtained by protein determination, finally the activity (mu mol/mg.min) of esterase or carboxylesterase is calculated, and each group of samples is repeated for 3 times.
1.2.3.3 glutathione S-transferase (GST) Activity assay
The assay was performed using glutathione S-transferase (GST) activity assay kit (from Solebao, BC 0350). GST catalyzes GSH to be combined with CDNB, and the light absorption peak wavelength of the combined product is 340 nm; the GST activity can be calculated by measuring the absorbance rising rate at the wavelength of 340nm, and each group of samples is repeated for 3 times.
2 results and analysis
2.1 synergistic Effect of combination of indoxacarb and Triflufenapyr on Nilaparvata lugens
The results were analyzed according to the grandsinope virulence factor method (table 1): the effective mass ratio of indoxacarb to trifluoro-benzene pyrimidine is 3: 1, 5: 1 and 9: 1, the co-toxicity coefficient range is 149.40-245.40, and the effective mass ratio has a synergistic effect. Wherein the combination of 5: 1 has remarkable synergistic effect, and the co-toxicity coefficient is 245.40, LC50The value was 3.54 mg/L.
TABLE 1 synergistic effect of indoxacarb and trifluoropyrimidine and combinations thereof on Nilaparvata lugens
2.2 Effect of the combination of indoxacarb and Trifluoropyrimidine on Nilaparvata lugens esterase
After the indoxacarb, the triflumuron and the indoxacarb-triflumuron (5: 1) combined sublethal dose are treated for 48 hours, the activity of the carboxylesterase in the body of the 3 rd nymph brown planthopper is inhibited. LC in combination of indoxacarb, trifluralin and indoxacarb trifluralin (5: 1)25At the dose, the esterase activities of 3-year-old nymphs of brown planthoppers are 0.7749 mu mol/mg-min, 1.8682 mu mol/mg-min and 1.7101 mu mol/mg-min respectively, which are 0.30, 0.72 and 0.66 times of the control activity of 2.5961 mu mol/mg-min (see figure 1). Can be used forThe conclusion is that both indoxacarb and trifluoro-benzene pyrimidine can inhibit the carboxylesterase enzyme activity in brown planthopper, so that the brown planthopper generates toxic reaction.
2.3 Effect of the combination of indoxacarb and Trifluoropyrimidine on Brown planthopper GST
As shown in figure 2, the combination of indoxacarb and indoxacarb-trifluoropyrimidine (5: 1) shows activation effect on glutathione S-transferase activity in the body of 3 th instar nymph of brown planthopper after 48h treatment, and the activation effect is shown in LC of the combination of indoxacarb-trifluoropyrimidine (5: 1)25Under the dosage, the glutathione S-transferase activity of 3-year-old nymphs of brown planthoppers is 0.6953 mu mol/mg-min and 0.6835 mu mol/mg-min, and is 1.16 times and 1.14 times of the contrast activity compared with the contrast activity of 0.6007 mu mol/mg-min; after the treatment of the sublethal dose of the trifluorobenzene pyrimidine, the activity of glutathione S-transferase in brown planthopper still shows inhibition, which is 0.82 times of the control activity.
3 small knot
LC for determining toxicity of synergistic combination of the invention to rice brown planthopper indoors50The value is 3.54mg/L, the cotoxicity coefficient CTC value reaches 245.40, and the combination of indoxacarb and trifluoro-benzene pyrimidine has obvious inhibition effect on the esterase activity of the brown planthopper of the rice and obvious activation effect on the enzymatic activity of glutathione S-transferase, and the result shows that the combination not only has small dosage but also has obvious synergistic effect on the inhibition of the brown planthopper. Therefore, the scheme of the invention is a theoretical basis, and the efficient novel synergistic combination for preventing and treating the brown planthopper of the rice is developed.
Example 1: in order to know the control effect of indoxacarb and trifluoropyrimidine on rice brown planthopper, a field test is carried out in a good flower Hongzhou test demonstration area in Huihuan county in 6 months in 2019, and a bucket-mixing spray test is carried out on indoxacarb and trifluoropyrimidine commercial medicines in a laboratory from ear emergence to grain filling period of rice
The test is carried out by setting 15 percent indoxacarb suspending agent (the using amount of the effective component is 67.5g a.i./hm)2) 10% of trifluorobenzene pyrimidine suspending agent (the using amount of the effective component is 37.5g a.i./hm)2) Indoxacarb-trifluoro-benzene pyrimidine combination (the dosage of the effective component is 48.75g a.i./hm)2) Clear water control for 4 treatments, repeat for each treatment3 times, 12 cells in total, each cell is randomly arranged, and the area of the cell is 30m2Spraying with 3WBJ-16DZ multifunctional electrostatic sprayer at 20/8 months. And (3) surveying 10 holes of rice and 2 groups of rice in each hole in 3, 7 and 14 days before and after application, beating the base of the rice and counting the number of fallen brown planthoppers in each hole.
The results are shown in table 2, the control effect of the combination of 3-14 d indoxacarb and trifluoro-benzene pyrimidine (5: 1) on brown planthopper reaches more than 90%, and the control effect of 15% indoxacarb suspending agent is obviously different from that of 10% trifluoro-benzene pyrimidine suspending agent and the combination of indoxacarb and trifluoro-benzene pyrimidine (5: 1). Wherein the control effect of the 10 percent of the trifluorobenzene pyrimidine suspending agent is better, and the control effect is more than 95 percent after the suspension is applied for 14 days; the indoxacarb and trifluoro-benzene pyrimidine (5: 1) are combined for the second time, and the 14d prevention effect is more than 90 percent after the pesticide is applied; compared with the control effect of 15 percent indoxacarb, the control effect is lower, and the control effect is between 50 and 70 percent after the indoxacarb is applied for 3 to 14 days. In addition, the dosage of the synergistic combination in field application is respectively reduced by 26.88g a.i./hm compared with single indoxacarb and trifluoropyrimidine2And 29.38g a.i./hm2。
TABLE 2 field control of Ningchong-Trifluprim combinations against Nilaparvata lugens
Of course, the above is only a specific application example of the present invention, and other embodiments of the present invention are also within the scope of the present invention, which is formed by equivalent substitution or equivalent transformation and processing of the combined dosage forms.
Claims (2)
1. A high-efficiency novel synergistic combination for preventing and treating brown planthopper of rice is characterized in that: comprises main effective components consisting of indoxacarb and trifluoro-benzene pyrimidine.
2. The highly effective novel synergistic combination for the control of brown planthopper in rice according to claim 1, wherein: the mass ratio of the indoxacarb to the trifluoro-benzene pyrimidine in the main effective component is 5: 1.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104430429A (en) * | 2013-09-24 | 2015-03-25 | 陕西韦尔奇作物保护有限公司 | Insecticidal composition containing trifluorobenzene pyrimidine and carbamates |
| CN107531700A (en) * | 2015-04-21 | 2018-01-02 | 日本化药株式会社 | Meso-ionic compound |
| IN201821023081A (en) * | 2018-06-20 | 2019-12-27 |
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- 2020-10-26 CN CN202011158374.8A patent/CN112088894A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104430429A (en) * | 2013-09-24 | 2015-03-25 | 陕西韦尔奇作物保护有限公司 | Insecticidal composition containing trifluorobenzene pyrimidine and carbamates |
| CN107531700A (en) * | 2015-04-21 | 2018-01-02 | 日本化药株式会社 | Meso-ionic compound |
| IN201821023081A (en) * | 2018-06-20 | 2019-12-27 |
Non-Patent Citations (2)
| Title |
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| 唐涛等: "多靶标杀虫剂——三氟苯嘧啶混配剂对水稻害虫的田间防治效果", 《植物保护》 * |
| 肖彩云等: "茚虫威与三氟苯嘧啶对贵州褐飞虱种群的协同作用及其解毒酶的影响", 《农药》 * |
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