CN112080390A - Genetic engineering fungus culture apparatus for it is biological experiments - Google Patents

Genetic engineering fungus culture apparatus for it is biological experiments Download PDF

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Publication number
CN112080390A
CN112080390A CN202010957499.0A CN202010957499A CN112080390A CN 112080390 A CN112080390 A CN 112080390A CN 202010957499 A CN202010957499 A CN 202010957499A CN 112080390 A CN112080390 A CN 112080390A
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CN
China
Prior art keywords
test tube
main shaft
base
lid
driving sleeve
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Pending
Application number
CN202010957499.0A
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Chinese (zh)
Inventor
于婧雅
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Shandong University
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Shandong University
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Publication date
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Priority to CN202010957499.0A priority Critical patent/CN112080390A/en
Publication of CN112080390A publication Critical patent/CN112080390A/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/16—Vibrating; Shaking; Tilting

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  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a genetically engineered bacteria culture device for biological experiments, which comprises a barrel body, a main shaft, a test tube supporting plate, a test tube stabilizing frame, a test tube, a cover body, a driving device and an oscillating device.

Description

Genetic engineering fungus culture apparatus for it is biological experiments
Technical Field
The invention relates to a biological experiment device, in particular to a genetic engineering bacteria culture device for biological experiments.
Background
The genetically engineered bacterium is a bacterium which produces a desired protein by introducing a target gene into a bacterium body and expressing the gene. Such as E.coli. In recent years, genetic engineering has begun to be carried out from laboratories to industrial production, and some rare drugs such as insulin, interferon, human growth hormone and the like are successively marketed, but from many researches, it is found that the culture and fermentation of the gene recombinant bacteria have characteristics of the gene recombinant bacteria. Factors such as control of the concentration of the nutrient source, control of the optimum growth conditions, and the like should be considered from the viewpoint of the culture engineering; biologically, major factors such as control of plasmid stability, control of plasmid copy number, improvement of transcription efficiency and translation efficiency, and secretion of metabolites to the outside of the bacterium should be considered. In the prior art, most of genetic engineering bacteria for biological experiments are cultured by adopting traditional biological test tubes and other equipment, the working efficiency is low, and the working effect is poor, so that an improvement space exists.
Disclosure of Invention
The present invention has been made to solve the above problems, and an object of the present invention is to provide a culture apparatus for genetically engineered bacteria for use in biological experiments.
The invention realizes the purpose through the following technical scheme:
the test tube stabilizing device comprises a barrel body, a main shaft, a test tube supporting plate, a test tube stabilizing frame, a test tube, a cover body, a driving device and an oscillating device, wherein the lower end of the main shaft is fixedly provided with the test tube supporting plate, the middle section of the main shaft is fixedly provided with the test tube stabilizing frame, the test tube stabilizing frame is provided with a plurality of through holes, the test tube supporting plate is provided with a groove corresponding to the through holes of the test tube stabilizing frame, the lower end of the test tube penetrates through the test tube stabilizing frame and then is positioned on a water pipe supporting plate, the main shaft is positioned in the barrel body and can rotate, the cover body is detachably connected with the upper end of the barrel body, the driving device is arranged in the cover body and drives the main shaft to rotate.
Further, drive arrangement comprises driving motor, drive gear, driven gear and driving sleeve, driving motor fixed set up in the lid, drive gear with driving motor's pivot fixed connection, driven gear with fixed connection between the driving sleeve, driving sleeve with rotate between the lid and be connected, driving sleeve with can dismantle fixed connection between the main shaft, driving sleeve with the coaxial fixed connection of driven gear, drive gear with driven gear meshing is connected.
Furthermore, the oscillating device is composed of an iron block, an armature core, an oscillating coil, a base and springs, wherein the iron block is fixedly arranged in the middle of the lower end of the barrel body, the base is positioned below the barrel body, a plurality of springs are arranged between the lower end of the barrel body and the upper end of the base, the armature core is fixedly arranged in the middle of the upper end surface of the base and positioned below the iron block, and the oscillating coil is wound on the armature core; the lower end of the base is provided with a sucker.
The invention has the beneficial effects that:
the invention is a genetic engineering bacteria culture apparatus for biological experiment, compared with the prior art, the invention can place more genetic engineering bacteria by arranging a plurality of test tubes on a test tube steady rest, can realize the centrifugation for culturing the genetic engineering bacteria by driving a plurality of test tubes to rotate by a driving device, and simultaneously, an oscillation device is arranged to keep the conditions required by the culture of the genetic engineering bacteria, complete a plurality of culture steps at one time, can also expand an electronic automation control system, reduce manual operation, facilitate the implementation of standardized experiment process, and have the value of popularization and application.
Drawings
Fig. 1 is a schematic sectional structure of the present invention.
FIG. 2 is a schematic view of the structure of the tube stabilizer of the present invention.
In the figure: 1-barrel body, 2-main shaft, 3-test tube supporting plate, 4-test tube stabilizing frame, 5-test tube, 6-cover body, 7-driving motor, 8-driving gear, 9-driven gear, 10-driving sleeve, 11-iron block, 12-armature core, 13-oscillating coil, 14-base, 15-spring and 16-sucker.
Detailed Description
The invention will be further described with reference to the accompanying drawings in which:
as shown in fig. 1 and 2: the test tube stabilizing device comprises a barrel body 1, a main shaft 2, a test tube supporting plate 3, a test tube stabilizing frame 4, a test tube 5, a cover body 6, a driving device and an oscillating device, wherein the lower end of the main shaft 2 is fixedly provided with the test tube supporting plate 3, the middle section of the main shaft 2 is fixedly provided with the test tube stabilizing frame 4, the test tube stabilizing frame 4 is provided with a plurality of through holes, the test tube supporting plate 3 is provided with a groove corresponding to the through holes of the test tube stabilizing frame 4, the lower end of the test tube 5 penetrates through the test tube stabilizing frame 4 and then is positioned on the test tube supporting plate 3, the main shaft 2 is positioned in the barrel body 1 and can rotate, the cover body 6 is detachably connected with the upper end of the barrel body 1, the driving device is arranged in the cover body 6 and drives the main shaft 2.
Further, drive arrangement comprises driving motor 7, drive gear 8, driven gear 9 and driving sleeve 10, driving motor 7 fixed set up in the lid 6, drive gear 8 with driving motor 7's pivot fixed connection, driven gear 8 with fixed connection between the driving sleeve 10, driving sleeve 10 with rotate between the lid 6 and be connected, driving sleeve 10 with can dismantle fixed connection between the main shaft 2, driving sleeve 10 with the coaxial fixed connection of driven gear 9, drive gear 8 with driven gear 9 meshing is connected.
Further, the oscillating device is composed of an iron block 11, an armature core 12, an oscillating coil 13, a base 14 and springs 15, wherein the iron block 11 is fixedly arranged in the middle of the lower end of the barrel body 1, the base 14 is positioned below the barrel body 1, a plurality of springs 15 are arranged between the lower end of the barrel body 1 and the upper end of the base 14, the armature core 12 is fixedly arranged in the middle of the upper end surface of the base 14, the armature core 12 is positioned below the iron block 11, and the oscillating coil 13 is wound on the armature core 12; the lower end of the base 14 is provided with a suction cup 16.
The foregoing shows and describes the general principles and features of the present invention, together with the advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims (4)

1. The utility model provides a genetic engineering fungus culture apparatus for it is biological experiments which characterized in that: including staving, main shaft, test tube layer board, test tube steady rest, test tube, lid, drive arrangement and oscillation device, the lower extreme of main shaft is fixed the test tube layer board, the middle section of main shaft is fixed to be set up the test tube steady rest, be provided with a plurality of through-holes on the test tube steady rest, be provided with on the test tube layer board with the recess that the through-hole position of test tube steady rest corresponds, the lower extreme of test tube passes be located behind the test tube steady rest on the test tube layer board, the main shaft is located in the staving and can rotate, the lid with can dismantle the connection between the upper end of staving, be provided with drive arrangement in the lid, the drive arrangement drive the main shaft rotates, the lower extreme of staving is provided with oscillation device.
2. The genetically engineered bacterium culturing apparatus according to claim 1, wherein: drive arrangement comprises driving motor, drive gear, driven gear and driving sleeve, driving motor fixed set up in the lid, drive gear with driving motor's pivot fixed connection, driven gear with fixed connection between the driving sleeve, driving sleeve with rotate between the lid and be connected, driving sleeve with can dismantle fixed connection between the main shaft, driving sleeve with the coaxial fixed connection of driven gear, drive gear with driven gear meshing connects.
3. The genetically engineered bacterium culturing apparatus according to claim 1, wherein: the vibrating device comprises an iron block, an armature core, a vibrating coil, a base and springs, wherein the iron block is fixedly arranged in the middle of the lower end of the barrel body, the base is positioned below the barrel body, a plurality of springs are arranged between the lower end of the barrel body and the upper end of the base, the armature core is fixedly arranged in the middle of the upper end face of the base, the armature core is positioned below the iron block, and the vibrating coil is wound on the armature core.
4. The genetically engineered bacterium culturing apparatus according to claim 3, wherein: the lower end of the base is provided with a sucker.
CN202010957499.0A 2020-09-13 2020-09-13 Genetic engineering fungus culture apparatus for it is biological experiments Pending CN112080390A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010957499.0A CN112080390A (en) 2020-09-13 2020-09-13 Genetic engineering fungus culture apparatus for it is biological experiments

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010957499.0A CN112080390A (en) 2020-09-13 2020-09-13 Genetic engineering fungus culture apparatus for it is biological experiments

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CN112080390A true CN112080390A (en) 2020-12-15

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3926733A (en) * 1972-09-27 1975-12-16 Tanabe Seiyaku Co Method of continuous automatic incubation of bacteria and determination of growth thereof, and apparatus therefor
CN206382042U (en) * 2017-01-16 2017-08-08 吴琼 A kind of biological culture test tube placing box
CN109136089A (en) * 2018-08-14 2019-01-04 郑州源冉生物技术有限公司 A kind of rotating biological incubator
CN110331082A (en) * 2019-07-25 2019-10-15 黄淮学院 A kind of anaerobe separator
CN110903962A (en) * 2019-12-20 2020-03-24 柴玉霜 Mushroom test tube fungus is culture apparatus in advance

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3926733A (en) * 1972-09-27 1975-12-16 Tanabe Seiyaku Co Method of continuous automatic incubation of bacteria and determination of growth thereof, and apparatus therefor
CN206382042U (en) * 2017-01-16 2017-08-08 吴琼 A kind of biological culture test tube placing box
CN109136089A (en) * 2018-08-14 2019-01-04 郑州源冉生物技术有限公司 A kind of rotating biological incubator
CN110331082A (en) * 2019-07-25 2019-10-15 黄淮学院 A kind of anaerobe separator
CN110903962A (en) * 2019-12-20 2020-03-24 柴玉霜 Mushroom test tube fungus is culture apparatus in advance

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Application publication date: 20201215