CN112075441A - Bactericidal composition containing agricultural antibiotic and trifloxystrobin - Google Patents
Bactericidal composition containing agricultural antibiotic and trifloxystrobin Download PDFInfo
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- CN112075441A CN112075441A CN201910508331.9A CN201910508331A CN112075441A CN 112075441 A CN112075441 A CN 112075441A CN 201910508331 A CN201910508331 A CN 201910508331A CN 112075441 A CN112075441 A CN 112075441A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/50—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids the nitrogen atom being doubly bound to the carbon skeleton
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
Abstract
The bactericidal composition contains the agricultural antibiotic and the trifloxystrobin as effective components. The agricultural antibiotic is one of tetramycin and ningnanmycin. The mass ratio of the agricultural antibiotic serving as an effective component to the trifloxystrobin in the pesticide composition is 1: 100-100: the pesticide composition has the dosage form of missible oil, a suspending agent, wettable powder, water dispersible granules, an aqueous emulsion, a microemulsion, granules, microcapsules and an aqueous solution, and can be widely used for preventing and treating diseases such as mosaic disease, small leaf disease, spot disease, anthracnose, downy mildew, epidemic disease, gummy stem blight, rice blast, bacterial wilt, soft rot, root rot, blight and the like caused by viruses, bacteria and fungi of fruit trees, vegetables, underground rhizomes, tobaccos, traditional Chinese medicinal materials and grain and cotton crops.
Description
Technical Field
The invention relates to the technical field of pesticide compounding, in particular to a bactericidal composition containing agricultural antibiotic and trifloxystrobin.
Background
The trifloxystrobin broad-spectrum bactericide is a novel fluorine-containing bactericide successfully developed by taking natural products Strobilurins as bactericide lead compounds; has the characteristics of high efficiency, broad spectrum, protection, treatment, eradication, permeation, systemic activity, rain wash resistance, long lasting period and the like; the bactericidal composition is effective to strains which generate resistance to 1, 4-demethylase inhibitors, benzamides, dicarboxamides and benzimidazoles, and has no cross resistance with the existing bactericides; has good activity on almost all fungal diseases (ascomycetes, basidiomycetes, oomycetes and fungi imperfecti) such as powdery mildew, rust disease, septoria nodorum, net blotch, downy mildew, rice blast and the like; besides the special effects on powdery mildew and leaf spot, the bactericidal composition has good activity on rust disease, downy mildew, damping off, apple anabaena and sclerotinia rot of colza; the fertilizer is safe to crops, and is safe to the environment because the fertilizer can be quickly degraded in soil and water; the trifloxystrobin has the properties of broad spectrum, penetration, rapid distribution and the like, is quickly absorbed by crops, and has upward systemic property, so the rainwash resistance is good, and the lasting period is long.
Tetramycin (tetramycin) is a fermentation metabolite of streptomyces ahygroscopicus parasitoides, has a wide bactericidal spectrum, and has extremely strong killing effects on twenty-six known pathogenic fungi of three major categories, namely flagellate fungi, ascomycete fungi, deuteromycete fungi and the like; the composition is suitable for preventing and treating various fungal and bacterial diseases of various crops; especially has special effects on the fungal diseases such as fruit tree rot, alternaria leaf spot, rice blast, soybean root rot, melon wilt, cotton verticillium wilt, jujube rust, grape white rot, panax notoginseng black spot, tea cake disease, forest rot, canker, gummosis, defoliation, seedling stage damping off and the like.
The ningnanmycin belongs to cytosine nucleoside peptide type broad-spectrum antibiotic bactericide, and has the functions of prevention and treatment. Can prolong the virus incubation period, destroy the structure of virus granules, reduce the concentration of virus granules and improve the virus resistance of plants so as to achieve the effect of preventing and treating virus diseases; meanwhile, the growth of fungal hyphae can be inhibited, and the plant body can be induced to generate resistance protein, so that the immunity of the plant body is improved.
In the actual process of agricultural production, the application of chemical agents is the most effective means for controlling plant diseases and insect pests, but the most easily generated problem for controlling diseases by chemical agents is the generation of disease resistance. In the aspect of medicament control of plant bacterial diseases, researches have been carried out for many years at home and abroad, and although some antibacterial medicaments begin to enter the practical stage, the chemically synthesized medicaments have the defects of long period, high cost, high toxicity, serious environmental pollution and the like, and can not meet the requirements of agricultural sustainable development. Moreover, continuous application of a single pesticide preparation at a high dose for a long time easily causes problems such as drug residue, environmental pollution, and development of drug-resistant fungi. In contrast, the efficient, low-toxicity and low-residue compound development and research has the advantages of low investment and short development period, is valued at home and abroad, and develops strength. On the basis of indoor screening and field tests, agricultural antibiotics are screened out to be compounded with trifloxystrobin, and the synergistic effect is obvious. And no report is made on the agricultural antibiotic and trifloxystrobin compounded pesticide composition and application at present.
Disclosure of Invention
Based on the above situation, the invention aims to provide a novel efficient pesticide composition, which can be widely used for preventing and treating diseases such as mosaic disease, small leaf disease, spot disease, anthracnose, downy mildew, epidemic disease, gummy stem blight, yellow dwarf disease, rice blast, bacterial wilt, soft rot, root rot and blight of fruit trees, vegetables, underground rhizomes, tobacco, traditional Chinese medicinal materials and grain and cotton crops, and particularly has excellent prevention and treatment effects on cucumber target spot and rice sheath blight.
The technical scheme of the invention is as follows:
a sterilization composition containing agricultural antibiotic and trifloxystrobin, the effective component of the pesticide composition contains agricultural antibiotic and trifloxystrobin;
further, the pesticide composition also comprises auxiliary components;
further, the agricultural antibiotic is selected from one of tetramycin and ningnanmycin;
further, the mass ratio of the agricultural antibiotic to the trifloxystrobin in the pesticide composition is 100: 1-1: 100;
further, the mass ratio of tetramycin to trifloxystrobin in the pesticide composition is 1: 40-1: 100, and the mass ratio of ningnanmycin to trifloxystrobin is 1: 50-1: 1;
further, the mass ratio of tetramycin to trifloxystrobin in the pesticide composition is 1: 60-1: 90;
further, the mass ratio of the ningnanmycin to the trifloxystrobin is 1: 30-2: 1;
further, the mass ratio of the agricultural antibiotic to the trifloxystrobin in the pesticide composition is 1: 79;
further, the mass ratio of the ningnanmycin to the trifloxystrobin is 1: 10.
The total weight of the agricultural antibiotic and the trifloxystrobin in the bactericidal composition accounts for 0.1-80% of the total weight of the preparation, and is preferably 0.1-20%.
Further, the pesticide auxiliary components in the bactericidal composition are carriers and auxiliaries.
The carrier is one or more of water, solvent or filler, and the water is preferably deionized water.
The solvent is selected from N, N-dimethylformamide, cyclohexanone, toluene, xylene, dimethyl sulfoxide, methanol, ethanol, trimethylcyclohexanone, N-octylpyrrolidone, ethanolamine, triethanolamine and isopropylamine. One or more of N-methyl pyrrolidone, propanol, butanol, ethylene glycol, diethylene glycol, ethylene glycol methyl ether, butyl ether, ethanolamine, isopropylamine, ethyl acetate or acetonitrile.
The filler is selected from one or a mixture of more of kaolin, diatomite, bentonite, attapulgite, white carbon black, starch or light calcium carbonate.
The auxiliary agent at least comprises a surfactant, and other functional auxiliary agents such as an antifreezing agent, a thickening agent, a stabilizing agent, a disintegrating agent, a defoaming agent and the like can be added according to different use occasions and requirements.
The surfactant is selected from one or four of an emulsifier, a dispersant, a wetting agent or a penetrating agent. The surfactant is a single agent or a compound preparation of a common nonionic surfactant or an anionic surfactant.
The other functional auxiliary agents are selected from one or more of antifreeze, thickening agent, stabilizing agent, disintegrating agent or defoaming agent.
The emulsifier is selected from one or more of agricultural milk 500# (calcium alkyl benzene sulfonate), OP series phosphate (nonylphenol polyoxyethylene ether phosphate), 600# phosphate (phenylphenol polyoxyethylene ether phosphate), styrene polyoxyethylene ether ammonium sulfate, magnesium alkyldiphenyl ether disulfonate, triethanolamine salt, agricultural milk 400# (benzyldimethylphenol polyoxyethylene ether), agricultural milk 700# (alkylphenol formaldehyde resin polyoxyethylene ether), Ning milk 36# (phenethylphenol formaldehyde resin polyoxyethylene ether), agricultural milk 1600# (phenethylphenol polyoxyethylene polypropylene ether), ethylene oxide-propylene oxide block copolymer, AEOP series (nonylphenol polyoxyethylene ether), BY series (castor oil polyoxyethylene ether), agricultural milk 33# (alkylaryl polyoxyethylene propylene oxide), span series (sorbitan monostearate), Tween series (sorbitan polyoxyethylene ether) or AEO series (fatty alcohol polyoxyethylene ether) A compound (I) is provided.
The dispersing agent is selected from one or a mixture of a plurality of polycarboxylate, lignosulfonate, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, calcium alkylbenzene sulfonate, naphthalene sulfonic acid formaldehyde condensate sodium salt, alkylphenol polyoxyethylene ether, fatty amine polyoxyethylene ether, fatty acid polyoxyethylene ether or glycerin fatty acid ester polyoxyethylene ether.
The wetting agent is selected from one or more of sodium dodecyl sulfate, sodium dodecyl benzene sulfonate, nekal BX, wetting penetrant F, saponin powder, silkworm excrement or soapberry powder.
The penetrating agent is selected from one or a mixture of more of penetrating agent JFC (fatty alcohol-polyoxyethylene ether), penetrating agent T (diisooctyl maleate sulfonate), azone or organic silicon.
The antifreezing agent is a mixture consisting of one or more of ethylene glycol, propylene glycol, glycerol or urea.
The thickening agent is selected from one or more of xanthan gum, polyvinyl alcohol, bentonite, carboxymethyl cellulose or magnesium aluminum silicate.
The stabilizer is selected from one or more of epoxidized soybean oil, epichlorohydrin, BHT, ethyl acetate and triphenyl phosphate.
The disintegrating agent is selected from one or more of bentonite, urea, ammonium sulfate, aluminum chloride, low-substituted hydroxypropyl cellulose, lactose, citric acid, succinic acid or sodium bicarbonate.
The defoaming agent is selected from one or a mixture of more of silicone oil, silicone compounds, C10-C20 saturated fatty acid compounds or C8-C10 fatty alcohol compounds.
Further, the composition may be formulated into a pesticidally acceptable formulation, including solid formulations, liquid formulations, seed treatment formulations, other formulations, according to methods well known to those skilled in the art;
further, the solid preparation is powder, dispersible tablets, granules, soluble powder, soluble granules, soluble tablets, milk powder, milk granules, water dispersible granules, wettable powder, micro-capsule granules, powder, macro-granules and water dispersible tablets;
further, the liquid preparation is microcapsule suspending agent, dispersible agent, missible oil, emulsion granules, oil emulsion, emulsion powder, aqueous emulsion, microemulsion, dispersible oil suspending agent, oil dispersion powder, suspending agent, suspoemulsion, soluble solution and ultra-low volume liquid;
further, the seed treatment preparation is a suspended seed coating agent, a seed treatment dry powder agent, a seed treatment dispersible powder agent, a seed treatment suspending agent and a seed treatment liquid agent;
further, the other preparations are bait, concentrated bait, gas preparation, aerosol, smoke agent;
furthermore, the preparation formulation is preferably missible oil, suspending agent, wettable powder, water dispersible granule, emulsion in water, microemulsion, granule and water aqua.
Further, the bactericidal composition is an emulsifiable concentrate preparation, and comprises the following components in parts by weight: 0.1-20 parts of agricultural antibiotic; 0.1-80 parts of trifloxystrobin; 10-30 parts of a conventional emulsifier; 20-50 parts of a conventional solvent; 1-5 parts of a conventional synergist. The specific production steps of the emulsifiable solution preparation are that the agricultural antibiotic and the trifloxystrobin which are effective components are added into a solvent to be completely dissolved, then the emulsifier and the synergist are added to be uniformly stirred to form uniform and transparent oily liquid, and the uniform and transparent oily liquid is filled to obtain the emulsifiable solution preparation of the composition.
Further, the bactericidal composition is a suspending agent and comprises the following components in parts by weight: 0.1-20 parts of agricultural antibiotic; 0.1-80 parts of trifloxystrobin; 5-20 parts of a dispersing agent; 1-5 parts of an antifreezing agent; 0.1-2 parts of a thickening agent; 0.1-0.8 part of defoaming agent; 0-10 parts of a penetration enhancer; 0.1-5 parts of a pH value regulator; and deionized water is used for complementing the balance. The specific production steps of the suspending agent are that other auxiliary agents are mixed, the mixture is uniformly mixed through high-speed shearing, the agricultural antibiotic and the trifloxystrobin which are effective ingredients are added, and the mixture is ball-milled in a ball mill for 2-3 hours, so that the particle diameter is below 5mm, and the suspending agent preparation of the composition can be prepared.
Further, the bactericidal composition is wettable powder and comprises the following components in parts by weight: 0.1-20 parts of agricultural antibiotic; 0.1-80 parts of trifloxystrobin; 3-10 parts of a dispersing agent; 1-5 parts of a wetting agent; filler and the balance. The wettable powder has the specific production steps as follows: the agricultural antibiotic as the effective component is mixed with trifloxystrobin, dispersant, wetting agent and filler according to the formula, the mixture is uniformly stirred in a stirring kettle and is uniformly mixed after passing through a jet mill, and the wettable powder of the composition can be prepared.
Further, the bactericidal composition is a water dispersible granule, and comprises the following components in parts by weight: 0.1-20 parts of agricultural antibiotic; 0.1-80 parts of trifloxystrobin; 3-10 parts of a dispersing agent; 1-10 parts of a wetting agent; 1-5 parts of a disintegrating agent; the filler makes up the balance. The water dispersible granule comprises the following specific production steps: according to the formula, the agricultural antibiotic as the active ingredient, the trifloxystrobin, the dispersant, the wetting agent, the disintegrant and the filler are uniformly mixed, crushed by an ultramicro jet mill, kneaded, added into a fluidized bed granulation dryer for granulation, drying and screening, and then sampled and analyzed, so that the water dispersible granule of the composition can be prepared.
Further, the bactericidal composition is an aqueous emulsion, and comprises the following components in parts by weight: 0.1-20 parts of agricultural antibiotic; 0.1-80 parts of trifloxystrobin; 3-30 parts of an emulsifier; 5-15 parts of a solvent; 2-15 parts of a stabilizer; 1-5 parts of an antifreezing agent; 0.1-8 parts of a defoaming agent; 0.2-2 parts of a thickening agent; and deionized water is used for complementing the balance. The specific production steps of the aqueous emulsion are as follows: firstly, adding agricultural antibiotic, trifloxystrobin, a solvent, an emulsifier and a cosolvent together to dissolve the agricultural antibiotic into a uniform oil phase; mixing part of water, antifreeze agent and other pesticide auxiliaries together to form a uniform water phase; adding the oil phase into the water phase while stirring at a high speed in a reaction kettle, slowly adding water until a phase inversion point is reached, starting a shearing machine to carry out high-speed shearing, adding the rest water, and shearing for about half an hour to form an oil-in-water emulsion, thus preparing the composition in the form of the emulsion in water.
Further, the bactericidal composition is a microemulsion and comprises the following components in parts by weight: 0.1-20 parts of agricultural antibiotic, 0.1-80 parts of trifloxystrobin, 10-30 parts of emulsifier, 1-8 parts of antifreezing agent, 0.5-10 parts of stabilizer and 20-50 parts of conventional solvent cosolvent. The agricultural antibiotic and the trifloxystrobin are completely dissolved by using the cosolvent, then the emulsifier, the antifreeze stabilizer and other components are added, the mixture is uniformly mixed, finally, the water is added, and the microemulsion can be prepared after the full stirring.
Further, the bactericidal composition is a granule, and comprises the following components in parts by weight: 0.1-20 parts of agricultural antibiotic, 0.1-80 parts of trifloxystrobin and 1-10 parts of binder; the carrier makes up the balance. Mixing the agricultural antibiotic as the effective component with trifloxystrobin, a binder, a carrier and other components according to the proportion of the formula, grinding the mixture in a sand grinding kettle, and then sending the ground mixture into a homogenizing mixer to be uniformly mixed to obtain a finished product.
Further, the sterilization composition is an aqueous agent and comprises the following components in parts by weight: 0.1-20 parts of agricultural antibiotic, 0.1-80 parts of trifloxystrobin and 0.1-30 parts of wetting dispersant; 2-8 parts of an antifreezing agent; 0.1-1 part of defoaming agent; 0.1-3 parts of a thickening agent; 5-15 parts of a spreader; and deionized water is used for complementing the balance. The agricultural antibiotic as effective component and trifloxystrobin are dissolved completely in cosolvent, dispersant, antifreezing agent, defoaming agent and other components are added and mixed evenly, and finally water is added and the mixture is stirred fully to obtain the finished product.
The compound bactericide has obvious synergistic effect, delays the generation of the drug resistance of the important, reduces the production cost and the use cost, and can be used for treating the resistant diseases. Can be widely used for preventing and treating mosaic disease, lobular disease, spot disease, anthracnose, downy mildew, epidemic disease, gummy stem blight, yellow dwarf disease, rice blast, bacterial wilt, soft rot, root rot, fusarium wilt and other diseases caused by viruses, bacteria and fungi of fruit trees, vegetables, underground rhizomes, tobacco, traditional Chinese medicinal materials and grain and cotton crops.
Detailed description of the preferred embodiments
In order to make the objects, technical solutions and advantages of the present invention more concise and clear, the present invention is described with the following specific embodiments, but the present invention is by no means limited to these embodiments. The following described examples are only preferred embodiments of the present invention, which can be used to describe the present invention and should not be construed as limiting the scope of the present invention. It should be understood that any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Example 1: combined toxicity experiments of agricultural antibiotics and trifloxystrobin in different proportions.
The test basis is as follows: the method refers to a hypha growth rate method (NY/T1156.3-2006) specified in pesticide indoor bioassay test criteria bactericide and combined action measurement (NY/T1156.6-2006) of bactericide mixing.
Test targets: cucumber target blight pathogen (Corynespora cassiicola).
The culture conditions are as follows: indoor PDA culture medium for cucumber target spot disease germs is continuously transferred and cultured at 25 +/-1 ℃ for 2 generations to serve as a test strain.
Test agents: 1.5 percent of tetramycin mother drug and 97 percent of trifloxystrobin original drug.
Setting a medicament: dissolving tetramycin (A) mother drug in water as a solvent, dissolving trifloxystrobin (B) raw drug in acetone as a solvent, respectively preparing 10000mg/L mother solution of test medicament, and placing the mother solution in a refrigerator for later use. Diluted with 0.05% aqueous tween 80 and set to 5 series of mass concentrations.
The test was repeated: each concentration treatment of the test agent was divided into 4 replicates and the fraction containing no active agent was used as a blank.
Data investigation and statistical analysis:
after 7 days of incubation of the plaque bacteria in the petri dishes, the test results were checked. The colony diameter (cm) was measured with a ruler, and the diameter was measured perpendicularly by the cross method for each colony, and the average value was taken (the diameter of the cake was subtracted by 5mm for each measurement).
The calculation formula is as follows:
control effect (%) [ (blank control lesion diameter-lesion diameter treated with drug)/blank control lesion diameter ] × 100
By EC50The smaller agent is the standard agent and has a virulence index of 100.
Measured virulence index (ATI) of the combination, EC of the standard agent50Measured EC of x 100/blend50
Theoretical virulence index (TTI) of the combination (virulence index of compound a alone x percent of compound a + virulence index of compound B alone x percent of compound B)/(percent of compound a + percent of compound B)
Co-toxicity coefficient (CTC) ═ ATI/TTI × 100
According to the Sun cloud Peel method: the co-toxicity coefficient (CTC) is more than or equal to 120, which is a synergistic effect; the co-toxicity coefficient (CTC) is less than or equal to 80 and is used as antagonism; a co-toxicity coefficient (CTC) of between 80 and 120 is additive.
Analysis of results
TABLE 1 determination of indoor combined toxicity of agricultural antibiotic and trifloxystrobin in different proportions on cucumber target spot pathogen
| Medicament and proportion | Regression equation of virulence | EC50(mg/L) | EC90(mg/L) | CTC |
| Tetramycin (A) | Y＝5.7353+0.7567X | 1.1067 | 5.2702 | - |
| Trifloxystrobin (B) | Y＝4.4504+1.3781X | 2.5051 | 21.3231 | - |
| A:B(1:40) | Y＝4.7786+1.5034X | 1.4036 | 9.9929 | 112.35 |
| A:B(1:60) | Y＝4.7745+1.3925X | 1.4518 | 12.0835 | 123.58 |
| A:B(1:79) | Y＝4.7251+1.544X | 1.5067 | 10.1875 | 127.64 |
| A:B(1:90) | Y＝4.6958+1.4748X | 1.6068 | 11.8911 | 123.12 |
| A:B(1:100) | Y＝4.5539+1.4903X | 1.9924 | 14.4309 | 101.39 |
As can be seen from the test results (see Table 1), the compounded test agents tetramycin and trifloxystrobin have higher activity on cucumber target spot disease, and the EC of tetramycin50A value of 1.1067mg/L, EC90A value of 5.2702 mg/L; EC of trifloxystrobin50A value of 2.5051mg/L, EC90A value of 21.3231 mg/L; EC for cucumber target spot pathogen mixed by two different proportions501.4036, 1.4518, 1.5067, 1.6068 and 1.9924mg/L respectively, and the co-toxicity coefficient CTC is 112.35, 123.58, 127.64, 123.12 and 101.39 respectively. The two medicaments have different degrees of synergy when the ratio of the two medicaments in the experimental design is 1: 60-1: 90, and the co-toxicity coefficient is larger than 120, wherein the co-toxicity coefficient of 1:79 is the largest, and the synergy is obvious.
Example 2: combined toxicity test of ningnanmycin and trifloxystrobin with different ratios
The test basis is as follows: NY/T1156.5-2006 "indoor bioassay of pesticides test guidelines part 5: broad bean leaf method for inhibiting rice sheath blight bacteria test, NY/T1156.6-2006 agricultural chemical indoor bioassay test criteria fungicide part 6: combined action assay of compounding.
Test targets: rhizoctonia solani (Rhizoctonia solani).
Preparing a test material: selecting broad bean susceptible varieties, shearing leaves with the same parts, consistent growth vigor and petioles, and placing the leaves in a culture dish for moisture preservation for later use.
Reagent to be tested: 40% of ningnanmycin mother drug and 97% of trifloxystrobin original drug.
Preparing a medicament: dissolving a ningnanmycin (A) original drug by using water as a solvent, dissolving a trifloxystrobin (B) original drug by using acetone as a solvent, diluting the dissolved solution by using a 0.05% Tween 80 aqueous solution, and setting the solution into 5 series of mass concentrations.
The test was repeated: each concentration treatment of the test agent was divided into 4 replicates and the fraction containing no active agent was used as a blank.
Medicament treatment: the side of the fungus cake with hypha and diameter of 5mm was inoculated to the center of the treated leaf. Inoculating 30 leaves in each treatment, inoculating pathogenic bacteria to the leaves for 24 hr, soaking in the prepared liquid medicine for 5s, draining off excessive liquid medicine, air drying, labeling, and culturing under moisture. Each treatment was repeated 4 times and a blank was set. The leaves are cultured in a moisture-preserving box with constant temperature and illumination.
Data investigation and statistical analysis:
the length and width of the lesion at each inoculation site were recorded by caliper measurement 7 days after the treatment with the lesion diameter (in mm) being expressed as the average of the length and width.
The calculation formula is as follows:
control effect (%) [ (blank control lesion diameter-lesion diameter treated with drug)/blank control lesion diameter ] × 100
By EC50The smaller agent is the standard agent and has a virulence index of 100.
Measured virulence index (ATI) of the combination, EC of the standard agent50Measured EC of x 100/blend50
Theoretical virulence index (TTI) of the combination (virulence index of compound a alone x percent of compound a + virulence index of compound B alone x percent of compound B)/(percent of compound a + percent of compound B)
Co-toxicity coefficient (CTC) ═ ATI/TTI × 100
According to the Sun cloud Peel method: the co-toxicity coefficient (CTC) is more than or equal to 120, which is a synergistic effect; the co-toxicity coefficient (CTC) is less than or equal to 80 and is used as antagonism; a co-toxicity coefficient (CTC) of between 80 and 120 is additive.
The test results are shown in Table 2
TABLE 2 Combined action test of Ningnanmycin and trifloxystrobin mixture on rice sheath blight disease
As can be seen from the test results (see Table 2), the combination of the test agents Ningnanmycin and trifloxystrobin has higher activity on rice sheath blight disease, and the EC of Ningnanmycin50A value of 11.2458 mg/L; EC of trifloxystrobin50A value of 9.3642 mg/L; EC for rice sheath blight disease by mixing two different proportions5010.3798, 9.0354, 7.6248, 5.6412, 6.9874, 7.3984, 8.3942 and 9.2345mg/L respectively, and the co-toxicity coefficient CTC is 107.92, 123.66, 146.09, 195.78, 155.73, 144.73/125.56 and 110.66 respectively. The two medicaments have the co-toxicity coefficient of more than 80 in the range of 1: 50-1: 1 in the experimental design, have addition or synergistic effect in different degrees, wherein the co-toxicity coefficient of 1:10 is the largest, and the synergistic effect is obvious.
Test of field drug effect
Example 3: field experiment for preventing and treating cucumber target spot
1. Test subjects, crops and varieties
Test subjects: target spot disease (Corynespora cassiicola)
And (3) test crops: cucumber (Cucumis sativus)
2. Crop cultivation and environmental conditions
The test is carried out in a Wuhan city agricultural academy vegetable Wuhu lake pesticide effect test base, the test land is flat, the soil fertility is moderate, sandy loam is adopted, the previous stubble is lettuce, and cucumber target spot disease occurs in the test area in recent years. Deep-furrow high-furrow cultivation is adopted, each furrow is provided with two rows, the density is about 3000 plants/mu, germination and seedling are accelerated in the middle ten days of 4 months, and the cucumber of 'Tangqiu No. 1' is planted in the last ten days of 5 months. The cultivation conditions and management measures of all the cells are uniform and consistent, and local production habits are followed.
3. Design and arrangement of experiments
Test agents: 12% tetramycin and trifloxystrobin suspending agent, 0.3% tetramycin aqueous agent serving as a contrast agent and 30% trifloxystrobin suspending agent.
And (3) test treatment: each experiment was set up for 6 treatments, 4 replicates.
The application method comprises the following steps: spraying the medicinal liquid. The total of three applications (6 months 13 days, 6 months 20 days, 6 months 27 days).
4. Investigation, recording and measuring method
Investigation time and number of times: the disease condition base observation is carried out before the first drug application, and the last investigation is carried out on the 9 th day after the last drug application. The total number of surveys was 2.
The investigation method comprises the following steps: four samples were taken randomly per cell and all leaves were investigated at each point and scored as the percentage of lesion area on each leaf to the total leaf area of the leaf.
Level 0: no disease;
level 1: the area of the lesion spots accounts for less than 5% of the area of the whole leaf;
and 3, level: the area of the lesion spots accounts for less than 6-10% of the area of the whole leaf;
and 5, stage: the area of the lesion spots accounts for less than 11-20% of the area of the whole leaf;
and 7, stage: the area of the lesion spots accounts for less than 21-50% of the area of the whole leaf;
and 9, stage: the lesion area accounts for more than 50% of the whole leaf area.
The drug effect calculation method comprises the following steps:
disease leaf rate (%). As the number of diseased leaves, survey of the total number of leaves X100
Disease index ∑ (number of diseased leaves at each stage × number of relative stages)/(total number of investigated leaves × 9) × 100
The control effect (%) is [ 1- (pre-drug disease index in the placebo area x post-drug disease index in the treatment area)/(post-drug disease index in the placebo area x pre-drug disease index in the treatment area) ] x 100.
5. Results and analysis
Table 2: test result of 12% tetramycin and trifloxystrobin suspending agent for preventing and treating cucumber target spot disease
And (3) evaluation of the medicament: through the control test of the cucumber target spot, the control effect of three dosages of the test medicament 12% tetramycin and trifloxystrobin suspending agent on the cucumber target spot is certain, the field control effect is increased along with the increase of the dosages, the best effective component dosage is 50.4 g/hectare, the control effect reaches 75.6%, the control effects of the other two dosages are 45 g/hectare and 39.6 g/hectare are respectively 71.2% and 65.1%, the control effect difference between 50.4 g/hectare and 45 g/hectare treated by the test medicament is not significant, the control effect difference between 39.6 g/hectare is an extremely significant level, and the control effect difference between 45 g/hectare and 39.6 g/hectare an extremely significant level, and is 5%. The control effect of the control agent 0.3% tetramycin water agent 2.25 g/hectare is 66.8%, the control effect difference with the test agent 50.4 g/hectare is extremely obvious, and the control effect difference with the test agents 45 g/hectare and 39.6 g/hectare is not obvious; the control effect of the other control medicament 30 percent of trifloxystrobin suspending agent 50g is 70.1 percent, the control effect difference with the test medicament 50.4 g/hectare is 5 percent of remarkable level, and the control effect difference with the test medicaments 45 g/hectare and 39.6 g/hectare is not remarkable. The two control agents had insignificant differences in control efficacy. Within the range of the test dosage, the test medicament is safe for cucumber growth.
Example 4: ningnanmycin and trifloxystrobin mixed field pesticide effect for preventing and treating rice sheath blight disease
The test field is selected in Yangzhou rice transplanting field, the fertilizer and water management is consistent, and the rice grows uniformly when the fertilizer is applied. 22 percent of ningnanmycin-trifloxystrobin suspending agent (1:10) prepared in a laboratory according to the effective component dosage of 50g/hm2、100g/hm2、150g/hm2At the tillering stage of riceUniformly spraying, wherein the first application is carried out in 7 months and 12 days, and the second application is carried out at intervals of 7 days (7 months and 19 days); the reference medicament is 8 percent of ningnanmycin water aqua active ingredient dosage of 50g/hm2The dosage of the effective component of 30 percent trifloxystrobin suspending agent is 100g/hm2And spraying clear water to the blank control.
The test preparation is according to GB/T17980.20-2000 pesticide field efficacy test criteria: the bactericide is used for preventing and treating rice sheath blight. The field efficacy investigation was conducted 7 days and 14 days after the second application. Grading according to the degree of harmful symptoms of rice leaf sheaths and leaves. Taking plants as a unit, sampling at 5 points by adopting a diagonal line, surveying 5 clusters at each point for 25 clusters, recording the total plant number, the disease plant number and each disease stage number, and calculating the disease plant rate, the disease plant rate control effect and the disease finger and disease finger control effect.
Grading the disease condition standard:
level 0: the whole plant is disease-free; level 1: the disease of the 4 th leaf and the leaf sheaths and leaves below; and 3, level: the disease of the 3 rd leaf and the following leaf sheaths and leaves; and 5, stage: the disease of the 2 nd leaf and the leaf sheaths and leaves below; and 7, stage: the scabies of the scabies and the scabies of the leaves below the scabies; and 9, stage: the disease is caused by the whole plant and died in advance.
Calculating the formula:
disease rate (%) ═ 100 x disease number of plants/total number of investigated plants;
disease index [ Σ (number of diseased plants at each stage × number of relative stage)/(total number of investigated plants × 9) ] × 100
The preventing and treating effect (%) is [ (disease index after blank group application-disease index after treatment group application)/disease index after blank group application ] × 100
Security survey
During the test, the crops are observed whether have phytotoxicity symptoms or not, if the phytotoxicity symptoms exist, the phytotoxicity degree is recorded, and the phytotoxicity symptoms (dwarfing, chlorosis, malformation and the like) of the crops are accurately described.
TABLE 4 field pesticide effect of Ningnanmycin and trifloxystrobin mixture for preventing and treating rice sheath blight
And (3) evaluation of the medicament: by passingThe field pesticide effect test of the rice sheath blight disease shows that the 22 percent ningnanmycin-trifloxystrobin suspending agent has good control effect on the rice sheath blight disease, the field control effect is increased along with the increase of the pesticide dosage, and the pesticide dosage of the effective component is 150g/hm282.01% and 88.38% respectively after 7 days and 14 days; the dosage is 50g/hm2、100g/hm2The control effect in 7 days is 74.43% and 79.53%, and the control effect in 14 days is 78.99% and 83.83%. The control effect is obviously higher than that of a single agent.
In conclusion, the bactericidal composition containing the agricultural antibiotic and the trifloxystrobin has good control effect on control targets, and is safe for target crops. The compound preparation not only improves the control effect, but also expands the control on bacteria, so the compound combination has very important significance in production practice.
Claims (10)
1. A bactericidal composition containing agricultural antibiotic and trifloxystrobin is characterized in that: the effective components of the bactericidal composition contain agricultural antibiotic and trifloxystrobin.
2. The germicidal composition of claim 1, further comprising: the agricultural antibiotic is one of tetramycin and ningnanmycin.
3. The germicidal composition of claim 1, wherein: the mass ratio of the agricultural antibiotic to the trifloxystrobin in the preparation is 100: 1-1: 100.
4. The germicidal composition of claim 1, wherein: the mass ratio of tetramycin to trifloxystrobin in the preparation is 1: 60-1: 90, and preferably 1: 79.
5. The germicidal composition of claim 1, wherein: the mass ratio of the ningnanmycin to the trifloxystrobin in the preparation is 1: 30-1: 2, and preferably 1: 10.
6. The germicidal composition of claim 1, wherein: the total weight of the agricultural antibiotic and the trifloxystrobin in the preparation accounts for 0.1-80% of the total mass of the preparation.
7. The germicidal composition of claim 1, wherein: the total weight of the agricultural antibiotic and the trifloxystrobin in the preparation accounts for 0.1-20% of the total mass of the preparation.
8. The germicidal composition of claim 7, wherein: the dosage form of the bactericidal composition is missible oil, suspending agent, wettable powder, water dispersible granules, emulsion in water, microemulsion, granules and water aqua.
9. Use of the fungicidal composition according to any one of claims 1 to 8 for controlling plant diseases; preferably, the plant diseases are mosaic diseases, small leaf diseases, spot diseases, anthracnose, downy mildew, epidemic diseases, gummy stem blight, yellow dwarf disease, rice blast, bacterial wilt, soft rot, root rot and fusarium wilt of fruit trees, vegetables, tobaccos, Chinese medicinal materials and grain and cotton crops caused by viruses, bacteria and fungi; preferably, the crop diseases are cucumber target spot or rice sheath blight.
10. A method for controlling plant diseases, characterized in that the fungicidal composition according to any one of claims 1 to 8 is applied to a plant growing area; preferably, the plant diseases are mosaic diseases, small leaf diseases, spot diseases, anthracnose, downy mildew, epidemic diseases, gummy stem blight, yellow dwarf disease, rice blast, bacterial wilt, soft rot, root rot and fusarium wilt of fruit trees, vegetables, tobaccos, Chinese medicinal materials and grain and cotton crops caused by viruses, bacteria and fungi; preferably, the plant disease is cucumber target spot or rice sheath blight.
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