CN112067408A - Automatic cell block preparation machine and method - Google Patents
Automatic cell block preparation machine and method Download PDFInfo
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- CN112067408A CN112067408A CN202011113004.2A CN202011113004A CN112067408A CN 112067408 A CN112067408 A CN 112067408A CN 202011113004 A CN202011113004 A CN 202011113004A CN 112067408 A CN112067408 A CN 112067408A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/42—Low-temperature sample treatment, e.g. cryofixation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/44—Sample treatment involving radiation, e.g. heat
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0099—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor comprising robots or similar manipulators
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2873—Cutting or cleaving
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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- G—PHYSICS
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- G01N2035/00465—Separating and mixing arrangements
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- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
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Abstract
The invention relates to an automatic cell block preparation machine and a method. The cell mass automatic preparation machine comprises a body, a centrifugal assembly, a temperature control assembly, a mechanical arm, a liquid path assembly, a desktop host and the like, wherein the centrifugal assembly, the temperature control assembly, the mechanical arm, the liquid path assembly, the desktop host and the like are arranged on the body. The cell block preparation machine and the cell block preparation tube with the matrix are used for separating and enriching cells in a cytological specimen on a plane after centrifugation and automatically preparing the cells into the cell block, so that the automation, the standardization and the high quality of the cell block preparation are realized.
Description
Technical Field
The invention relates to the technical field of medical equipment, in particular to an automatic cell block preparation machine and method.
Background
Cytopathology diagnoses a variety of diseases and assesses health by analyzing cells or small cell masses.
The most commonly used technique in cytopathology is cell smear. Cell smear is a process of coating cells or small cell masses on a glass slide, staining with a diagnostic reagent, and observing the morphology of the cells under a microscope to diagnose various diseases or to evaluate health. The cell smear method has obvious limitations, which are mainly shown in that: cell smears are unable to replicate, once stained with one diagnostic reagent, it is difficult to perform additional staining and detection simultaneously; detection of immunohistochemistry and molecular pathology is difficult; factors such as blood, protein precipitates, mucus interference and cell accumulation make the morphology and structure of cells unclear; cells are difficult to interpret due to stress distortion in the process of flaking; not long-term storage, etc.
The cell block is a bridge leading to the future of cytopathology, and is made into a cell wax block after dehydration, paraffin embedding and other procedures. The cell wax block is cut to prepare cell slices, and the cells are further dyed and then diagnosed. Cell sections have many advantages over cell smears: the cells are not deformed, the structure is clear, and the observation and the interpretation are easy; the arrangement of cells can be clearly observed like a biopsy tissue section, and therefore, is also called a micro-biopsy; the cell mass can be cut into slices of a plurality of cells or small cell masses for analysis; the special staining, immunocytochemistry staining, molecular pathology detection and the like are easy to perform; can be preserved for a long time for further diagnosis and research.
There are many methods to make cell mass, and the method is divided into direct method and indirect method, the direct method is to use the ordinary centrifugal tube to centrifugate and precipitate, then suck and remove the supernatant fluid, then take out the sediment, and then make into the wax mass with the conventional pathological technology method; the indirect method is a method in which a substance capable of aggregating cells is added to a cell pellet, the cells are aggregated, and then the aggregated cells are taken out to prepare a cell mass. However, these prior art methods all have different drawbacks, the most obvious of which are: the cell blocks are prepared one by one manually, which consumes time and labor; the preparation of cell blocks lacks standardization, normalization and quality control; it is not suitable for preparing a cell mass in the case of a small number of cells or cell masses; the position of the target cell (pathological cell) in the cell wax block is dispersed and uncertain, the pathological cell is difficult to cut when slicing, or the distribution surface of the cell on the slice is small and the density is low; the wax blocks are irregular in size and shape, etc.
Disclosure of Invention
The invention provides an automatic cell block preparation machine and a method. The automatic cell block preparing machine mainly comprises a body, a centrifugal assembly, a temperature control assembly, a liquid path assembly, a mechanical arm, a desktop host and the like, wherein the centrifugal assembly, the temperature control assembly, the liquid path assembly, the mechanical arm and the desktop host are arranged on the body, the centrifugal assembly is positioned on the lower portion of the body, the mechanical arm is positioned on the upper portion, and the host is positioned on the. Centrifugal component includes rotor, hanging cup, adapter and cell piece preparation pipe, is equipped with the embedding box screens on the rotor, and the cell piece preparation pipe is both ends open-ended cylindrical hollow tube, and the upper end internal diameter is big, and the lower extreme internal diameter is little, and the extexine at both ends has the external screw thread, and centrifuging tube bottom lid is flat, and the inside wall top layer has the internal thread, can screw up the interlock with the centrifuging tube external screw thread, forms cylindrical airtight space. The upper opening end of the cell block preparation tube is provided with a clamping shaft, and the bottom cover is provided with clamping teeth. The bottom of the adapter is provided with a semiconductor temperature control assembly, an iron block and a cell block preparation tube installation clamping groove, so that the cell block preparation tube can be heated/refrigerated, the centrifugal tube is inclined under the magnetic action of the electromagnet, the cell block preparation tube is supported and fixed, and the centrifugal tube cover is taken down when the mechanical arm rotates the centrifugal tube. The temperature control component is mainly made of semiconductor materials, has a heating/refrigerating bidirectional function, and can be switched between a heating/refrigerating physical state and a refrigerating physical state. The mechanical arm is provided with a bayonet for clamping the cell block preparation tube and a piston, and can be used for screwing off the cell block preparation tube and pushing out the cell block. The method for making cell block mainly uses the substance whose state (liquid state and solid state) can be changed with temp. as matrix material, and adopts the heated substance as medium for centrifugal separation after the matrix is liquefied, and can be used as supporting material after cooled and solidified. The matrix material comprises: agar, agarose, gelatin, matrigel, etc., by centrifugation of the cell block preparation tube (matrix), cells are enriched on one plane and diseased cells are distributed in the lowermost layer.
The invention has the beneficial effects that: (1) the cell mass automatic preparation machine and the cell mass preparation method realize the automatic preparation of the cytological specimen and the tiny tissue fragments obtained by the surgical method into the cell mass.
(2) Realizes the automation, standardization and quality control of the clinical cell block preparation.
(3) The cell block preparation tube and the matrix material are used, and a small amount of cells and tissue fragments can be efficiently enriched on one plane of the matrix through a centrifugal principle, so that a trace amount of cells can be prepared into the cell block.
(4) By the centrifugal separation of the matrix material, pathological cells and cell clusters (large cells and cells with high nuclear plasma ratio) are distributed in the lowest layer, and are cut off firstly when slices are manufactured to manufacture cell slices, so that the positive rate of diagnosis is improved.
(5) The section prepared by the method has large observable cell distribution area and abundant cells, and improves the diagnosis quality.
(6) The cell block prepared by the method has regular shape and definite cell distribution position, and is beneficial to cytopathology quality control and standardized operation.
Drawings
FIG. 1 is a schematic diagram of an automatic cell block preparation machine according to the present invention.
Fig. 2 is a schematic structural view of the centrifugal module of the present invention.
FIG. 3 is a schematic view showing the structure of a cell preparation tube according to the present invention.
Fig. 4 is a schematic diagram of the adapter structure of the present invention.
Fig. 5 is a schematic connection diagram of the fluid path assembly of the present invention.
FIG. 6 is a flow chart of a method of preparing a cell block according to the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, it should be noted that the terms "upper", "lower", "left", "right", "inner", "outer", etc. indicate orientations or positional relationships based on those shown in the drawings, which are merely for convenience of description and simplicity of description, but do not mean or imply that the designated devices or elements must have a specific orientation, and furthermore, the terms "mounted" and "connected" in the description of the present invention should be construed broadly.
The technical scheme of the invention is further described in detail by combining the drawings and the embodiments in the specification.
As shown in fig. 1, the automatic cell block preparation machine of the present invention comprises a body 1, and main components such as a centrifugal assembly 2, a temperature control assembly 41, a mechanical arm 5, a host machine 6, etc. arranged in the body, and further comprises a sample adding needle 7, a reagent bottle 8, a storage tank 9, etc., wherein a confluence tank 10 is arranged above a rotor. The body 1 is a box structure, the centrifugal assembly 2 is positioned at the lower part of the body, the mechanical arm 5 is positioned at the upper part of the body, and the desktop host 6 is positioned at the front lower part.
As shown in fig. 2, the centrifuge assembly 2 comprises a hanging cup 22, a rotor 23, a cell block preparation tube 3 and an adapter 4; the rotor 23 is a disc-shaped structure, and a confluence tank 10 and an embedding box clamping position 21 are arranged above the rotor.
As shown in FIG. 3, the cell block preparation tube 3 is a cylindrical hollow tube with two open ends, the inner diameter of the upper end is large, the inner diameter of the lower end is small, the outer surface layers at the two ends are provided with external threads, the inner side wall surface layers of the centrifuge tube bottom cover 31 and the top cover 32 are provided with internal threads, and the cell block preparation tube can be screwed and occluded with the external threads of the cell block preparation tube 3 to form a cylindrical closed space. The bottom cover 31 is flat, which is beneficial to enriching cells on a plane after centrifugation. The upper opening end of the cell block preparation tube 3 is provided with a clamping shaft 34, and the bottom cover is provided with a clamping tooth 33. The cell block preparation tube 3 is previously filled with a matrix material.
As shown in fig. 4, the bottom of the adapter 4 is provided with a semiconductor temperature control assembly 41, an iron block 42 and a cell block preparation tube installation clamping groove 43, the semiconductor temperature control assembly 41 can heat and refrigerate the cell block preparation tube 3, the iron block 42 tilts the centrifuge tube under the magnetic action of the electromagnet, so that the supernatant in the centrifuged cell preparation tube 3 flows into the confluence tank 10, and the cell block preparation tube installation clamping groove 43 is used for supporting and fixing the cell block preparation tube, so that the mechanical arm 5 is favorable for screwing off the cell preparation tube 3 and separating the cell preparation tube from the bottom cover thereof.
As shown in FIG. 5, the liquid path assembly is connected in such a manner that a reagent bottle 81 and a reagent bottle 82 are connected with a sample adding needle 7 through a liquid guide tube via a diaphragm liquid pump 84 and an electromagnetic valve 85, a reagent is added into the cell preparation tube 3, the liquid in the cell preparation tube 3 flows into the confluence pool, the inlet end of a liquid discharge tube is connected with the confluence pool, and the liquid discharge tube is connected into a waste liquid bottle via the diaphragm liquid pump 84 and the electromagnetic valve 85.
As shown in fig. 6, the cell block preparation flow chart includes: specimen collection S1, cell pellet preparation tube mounting S2, centrifugation S3, supernatant removal S4, cell fixation S5, matrix liquefaction S6, centrifugation S7, matrix solidification S8, removal of cell preparation tube and ejection of cell pellet S9.
The technical solution of the present invention will be further described by describing the operation of the automatic cell block preparation machine in detail. The working process of the fully automatic immunohistochemical system comprises the following steps.
(1) Collecting a specimen: the top cap 32 of the cell block preparation tube 3 is unscrewed, and the cytological specimen is added to the cell block preparation tube 3.
(2) Cell block preparation tube installation: the cell pellet preparation tube 3 (containing the cytological specimen) is mounted in the adapter 4 and the latch 33 on the bottom cap is inserted into the latch slot 43 of the adapter.
(3) Centrifuging: the cells were enriched on top of the solid matrix in the cell pellet preparation tube 3 by centrifugation at 1500 rpm (400 g) for 5 minutes.
(4) Removing the supernatant: the cell mass preparation tube 3 is gradually tilted to the horizontal by attracting the iron mass 41 in the adapter by the electromagnet, the supernatant in the cell mass preparation tube 3 flows into the confluence tank 10 along the tube mouth, the supernatant is completely removed, and the supernatant is further sucked into the waste liquid bottle 83 by the diaphragm liquid pump 84.
(5) Cell fixation: the cell fixing solution 81 was added to the cell preparation tube 3 through the sample addition needle 7, and the cells were fixed for 10 minutes.
(6) Matrix liquefaction: the substrate in the cell block preparation tube 3 was heated to 75 ℃ by the semiconductor temperature control member 41 at the bottom of the adapter 4 to liquefy the substrate.
(7) Centrifuging: the cell preparation tube 3 after cell fixing is centrifuged for 5 minutes at 1500 rpm (400 g), and the cells are separated and enriched on one plane at the lower end of the matrix under the separation action of the matrix, and the pathological cells are distributed in the lowermost layer due to large volume.
(8) Solidifying the matrix: the semiconductor temperature control component 41 is used for refrigerating the cell preparation tube 3 of the cells, so that the matrix is solidified, and the separated cells are supported and fixed at a specific spatial position of the matrix.
(9) Taking out cell blocks: the mechanical arm 5 moves and abuts the bayonet 51 on the clamping shaft at the upper end of the cell preparation tube 3, applies a rotational acting force to rotationally take down the cell block preparation tube 3 in the adapter 4, moves the cell block preparation tube to the position right above the clamping groove 21 of the embedding box, and pushes the cell block out of the embedding box by the piston 52.
The whole process is accurately controlled by the host 7.
It should be noted that the above description of the specific embodiments of the present invention is only for the purpose of illustrating the technical lines and features of the present invention, and the purpose of the present invention is to enable those skilled in the art to understand the content of the present invention and to implement the present invention, but the present invention is not limited to the above specific embodiments. It is intended that all such changes and modifications as fall within the scope of the appended claims be embraced therein.
Claims (10)
1. An automatic cell block preparation machine and a method thereof are characterized by comprising the automatic cell block preparation machine and a method for preparing cell blocks based on the automatic cell block preparation machine;
the automatic cell block preparation machine is characterized by comprising a body, a centrifugal assembly, a temperature control assembly, a mechanical arm, a liquid path assembly, a desktop host and the like, wherein the centrifugal assembly, the temperature control assembly, the mechanical arm, the liquid path assembly, the desktop host and the like are arranged on the body;
the centrifugal assembly is characterized by comprising a rotor, a suspension cup, an adapter and a cell block preparation tube;
the temperature control assembly is characterized by being made of semiconductor materials, having a heating/refrigerating bidirectional function and being capable of being switched between a heating physical state and a heating physical state;
the method is characterized in that the method for preparing the cytological specimen into the cell mass is realized by relying on the automatic cell mass preparation machine.
2. The automated cell mass preparation machine and method of claim 1, wherein the rotor is a disk-shaped structure with a sink positioned above it.
3. The automatic cell block preparation machine and method as claimed in claim 1, wherein the cell block preparation tube is a cylindrical hollow tube with two open ends, the inner diameter of the upper end is large, the inner diameter of the lower end is small, the outer surface layers of the two ends are provided with external threads, the inner side surface layers of the top cover and the bottom cover are provided with internal threads, and the internal threads can be screwed with the external threads of the centrifuge tube to form a cylindrical closed space.
4. The automated cell pellet preparation machine and method of claim 1 wherein the upper end of the pellet preparation tube is provided with a latch shaft and the bottom cover is provided with a latch.
5. The automated cell mass preparation machine and method of claim 1, wherein the cell mass preparation tube is pre-filled with matrix material.
6. The automated cell block preparation machine and method of claim 1, wherein the bottom of the adapter is provided with a semiconductor temperature control assembly, an iron block, and a cell block preparation tube mounting slot.
7. The automated cell mass preparation machine and method of claim 1, wherein the robotic arm has a bayonet for holding the cell mass preparation tube and a plunger.
8. The automatic cell mass preparation machine and method as claimed in claim 1, wherein the matrix material is a substance whose physical state (liquid state and solid state) changes with temperature, and the cells separated from the liquid matrix are fixed at a specific spatial position by heating to liquefy the matrix material, using the liquefied matrix material as a medium for centrifugal separation, and using the liquefied matrix material as a support material after cooling.
9. The automated cell mass preparation machine and method of claim 1, wherein the matrix material comprises: agar, agarose, gelatin, matrigel, and the like.
10. The automated cell mass preparation machine and method of claim 1, wherein the cells are concentrated in a single plane and the diseased cells are distributed in the lowermost layer by centrifugation of the cell mass preparation tube (matrix).
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011113004.2A CN112067408A (en) | 2020-10-16 | 2020-10-16 | Automatic cell block preparation machine and method |
| CN202110222151.1A CN112729999A (en) | 2020-10-16 | 2021-02-28 | Automatic cell block preparation machine and method |
| PCT/CN2021/122474 WO2022078235A1 (en) | 2020-10-16 | 2021-09-30 | Automatic cell block preparation machine and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011113004.2A CN112067408A (en) | 2020-10-16 | 2020-10-16 | Automatic cell block preparation machine and method |
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| CN112067408A true CN112067408A (en) | 2020-12-11 |
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| CN202011113004.2A Pending CN112067408A (en) | 2020-10-16 | 2020-10-16 | Automatic cell block preparation machine and method |
| CN202110222151.1A Pending CN112729999A (en) | 2020-10-16 | 2021-02-28 | Automatic cell block preparation machine and method |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202110222151.1A Pending CN112729999A (en) | 2020-10-16 | 2021-02-28 | Automatic cell block preparation machine and method |
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| WO (1) | WO2022078235A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022078235A1 (en) * | 2020-10-16 | 2022-04-21 | 赵稳兴 | Automatic cell block preparation machine and method |
| WO2023082079A1 (en) * | 2021-11-10 | 2023-05-19 | Leica Biosystems Nussloch Gmbh | Cassette and embedding method using same |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1967193A (en) * | 2005-11-18 | 2007-05-23 | 上海昂佰生物医学科技有限公司 | Cell processing kit and using method therefor |
| CN102174395B (en) * | 2011-01-30 | 2013-08-21 | 中国科学院广州生物医药与健康研究院 | Automation augmentation and culture system of induced pluripotent stem cells |
| CN104568539B (en) * | 2014-12-16 | 2017-05-10 | 何向锋 | Automatic histocyte separator |
| CN112067408A (en) * | 2020-10-16 | 2020-12-11 | 昆明东环科技有限公司 | Automatic cell block preparation machine and method |
-
2020
- 2020-10-16 CN CN202011113004.2A patent/CN112067408A/en active Pending
-
2021
- 2021-02-28 CN CN202110222151.1A patent/CN112729999A/en active Pending
- 2021-09-30 WO PCT/CN2021/122474 patent/WO2022078235A1/en unknown
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022078235A1 (en) * | 2020-10-16 | 2022-04-21 | 赵稳兴 | Automatic cell block preparation machine and method |
| WO2023082079A1 (en) * | 2021-11-10 | 2023-05-19 | Leica Biosystems Nussloch Gmbh | Cassette and embedding method using same |
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| WO2022078235A1 (en) | 2022-04-21 |
| CN112729999A (en) | 2021-04-30 |
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