CN112063766A - Polysaccharide degradation method by liquid-phase pulse discharge plasma - Google Patents
Polysaccharide degradation method by liquid-phase pulse discharge plasma Download PDFInfo
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- CN112063766A CN112063766A CN201911334349.8A CN201911334349A CN112063766A CN 112063766 A CN112063766 A CN 112063766A CN 201911334349 A CN201911334349 A CN 201911334349A CN 112063766 A CN112063766 A CN 112063766A
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- C—CHEMISTRY; METALLURGY
- C13—SUGAR INDUSTRY
- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
- C13K13/00—Sugars not otherwise provided for in this class
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Abstract
The invention relates to a polysaccharide degradation method, in particular to a liquid-phase pulse discharge plasma polysaccharide degradation method. The method comprises the following steps: placing polysaccharide solution to be degraded into a liquid-phase pulse discharge plasma reactor, immersing a discharge electrode in the polysaccharide solution, adding metal ions, dissolving the metal ions into the polysaccharide solution, and performing liquid-phase pulse discharge plasma degradation treatment to obtain polysaccharide degradation liquid; and carrying out alcohol precipitation treatment on the polysaccharide degradation liquid, carrying out centrifugal separation to obtain an alcohol precipitate, heating the alcohol precipitate to remove residual absolute ethyl alcohol, and carrying out freeze drying to obtain the oligosaccharide freeze-dried powder. Compared with the method without adding metal ions, the metal ions obviously promote the degradation effect; the degradation is carried out at normal temperature, and compared with the traditional degradation technology which needs heating treatment, the energy consumption is reduced. Moreover, the degradation reaction is not violent and is easy to control; pulse discharge is adopted, so that the energy consumption is low, and the degradation treatment cost is low.
Description
Technical Field
The invention relates to a polysaccharide degradation method, in particular to a liquid-phase pulse discharge plasma polysaccharide degradation method.
Background
The polysaccharide substance is a natural active substance with obvious biological activity, is widely applied to medicines, health-care products and functional foods, and has wide market prospect. However, the natural polysaccharides are macromolecular polysaccharides, and the development of polysaccharide products is greatly limited due to the defects of high viscosity, poor solubility, low activity and the like. Therefore, the degradation treatment of the natural macromolecular polysaccharide to prepare the high-functional active oligomeric polysaccharide has important significance for the development of the polysaccharide industry.
The pulse discharge plasma is a novel advanced oxidative degradation technology, has the advantages of high efficiency, low consumption and the like, and is used for polysaccharide degradation processing. Research shows that the pulse discharge plasma technology has certain degradation effect on polysaccharide. However, the technology has incomplete polysaccharide degradation effect and cannot completely degrade macromolecular polysaccharide. Therefore, how to enhance the polysaccharide degradation effect of the pulsed discharge plasma is an urgent problem to be solved.
Disclosure of Invention
The invention provides a polysaccharide degradation method by using liquid-phase pulse discharge plasma, aiming at solving the technical problems and improving the polysaccharide degradation effect of pulse discharge plasma.
In order to achieve the above object, the present invention provides a method for degrading polysaccharides by liquid phase pulse discharge plasma, comprising the steps of:
placing polysaccharide solution to be degraded into a liquid-phase pulse discharge plasma reactor, immersing a discharge electrode in the polysaccharide solution, adding metal ions, dissolving the metal ions into the polysaccharide solution, and performing liquid-phase pulse discharge plasma degradation treatment to obtain polysaccharide degradation liquid;
and carrying out alcohol precipitation treatment on the polysaccharide degradation liquid, carrying out centrifugal separation to obtain an alcohol precipitate, heating the alcohol precipitate to remove residual absolute ethyl alcohol, and carrying out freeze drying to obtain the oligosaccharide freeze-dried powder.
The discharge electrode is in direct contact with the polysaccharide solution.
And dissolving metal ions in the polysaccharide solution, and performing coordination reaction with the polysaccharide.
The metal ions are dissolved in different concentrations.
The liquid phase pulse discharge plasma reactor has the pulse discharge voltage of 10-60kV, the power of 350-.
The polysaccharide degradation treatment in the liquid-phase pulse discharge plasma reactor is carried out at normal temperature and normal pressure.
Compared with the prior art, the invention has the following advantages:
(1) compared with the method without adding metal ions, the metal ions obviously promote the degradation effect;
(2) the degradation is carried out at normal temperature, and compared with the traditional degradation technology which needs heating treatment, the energy consumption is reduced. Moreover, the degradation reaction is not violent and is easy to control;
(3) pulse discharge is adopted, so that the energy consumption is low, and the degradation treatment cost is low.
Drawings
FIG. 1 is a graph showing the degradation rate of chitosan after different metal ions are dissolved in the chitosan solution of examples 1-3.
FIG. 2 shows the dissolution of Cu in the chitosan solution of example 22+After the metal ion, with Cu2+Concentration change and a change trend chart of the degradation rate of the chitosan.
Detailed Description
The present invention will be described in further detail with reference to examples. The following examples are only specific examples of the present invention, but the design concept of the present invention is not limited thereto, and any insubstantial modifications of the present invention using the design concept shall fall within the scope of infringing upon the protection of the present invention.
Example 1
Preparing 0.3 wt% chitosan solution, placing the prepared polysaccharide solution into a liquid phase pulse discharge plasma reactor, wherein the lowest liquid level is higher than the height of a discharge electrode plate, and 5mM Fe2+Dissolved in polysaccharide solution, the liquid phase pulse discharge plasma reactor is set with pulse discharge voltage of 60kV, power of 350W, pulse width of 40ns, pulse frequency of 4.67 kHz, and degradation treatment is carried out for 180 min. After the degradation treatment is completed, anhydrous ethanol with three times of volume is added into the polysaccharide degradation solution to separate out the polysaccharide. Centrifuging to obtain alcohol precipitate, heating to remove residueAnd (5) reserving absolute ethyl alcohol, and freeze-drying to obtain the oligosaccharide freeze-dried powder. Compared with the method without adding, the degradation rate is improved by 30 percent.
Example 2
Example 1 the metal ion was 5mM Cu2+The liquid phase pulse discharge plasma reactor was set to have a pulse discharge voltage of 10kV, a power of 500W, a pulse width of 20ns, and a pulse frequency of 3kHz, and was subjected to degradation treatment for 150 min, as in example 1. Compared with the non-additive agent, the degradation rate is improved by 37.3 percent.
Example 3
Example 1 the metal ion is 5mM Zn2+The liquid phase pulse discharge plasma reactor was set to have a pulse discharge voltage of 30kV, a power of 600W, a pulse width of 30ns, and a pulse frequency of 3kHz, and was subjected to degradation treatment for 180 min, as in example 1. Compared with the non-additive agent, the degradation rate is improved by 14.4 percent.
Example 4
Cu in example 12+The concentrations were 1, 5, 10 and 500 mM, respectively, and the liquid phase pulse discharge plasma reactor was degraded for 240 min with a pulse discharge voltage of 10kV, a power of 600W, a pulse width of 30ns and a pulse frequency of 3kHz, as in example 1. Compared with the non-additive, the degradation rate is respectively improved by 23.7 percent, 30 percent, 42.8 percent and 42.5 percent.
The above description is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto,
any person skilled in the art should be able to substitute or change the technical solution of the present invention and its inventive concept within the technical scope of the present invention, and all the equivalents and modifications thereof are covered by the protection scope of the present invention.
Claims (7)
1. The polysaccharide degradation method is characterized in that polysaccharide solution to be degraded is placed in a liquid-phase pulse discharge plasma reactor, a discharge electrode is immersed in the polysaccharide solution, metal ions are added, the metal ions are dissolved in the polysaccharide solution, and liquid-phase pulse discharge plasma degradation treatment is carried out to obtain polysaccharide degradation liquid;
and carrying out alcohol precipitation treatment on the polysaccharide degradation liquid, carrying out centrifugal separation to obtain an alcohol precipitate, heating the alcohol precipitate to remove residual absolute ethyl alcohol, and carrying out freeze drying to obtain the oligosaccharide freeze-dried powder.
2. The method of claim 1, wherein the discharge electrode is in direct contact with the polysaccharide solution.
3. The method of claim 1, wherein the polysaccharide solution is dissolved with metal ions to coordinate with polysaccharide.
4. The method of claim 1, wherein the dissolved metal ions are Cu2+、Fe2+、Zn2+。
5. The method of claim 1, wherein different concentrations of metal ions are dissolved in the polysaccharide.
6. The method as claimed in claim 1, wherein the liquid phase pulse discharge plasma reactor has a pulse discharge voltage of 10-60kV, a power of 350-600W, a pulse width of 20-40ns, a pulse frequency of 3-4.67 kHz, and a degradation time of 150-240 min.
7. The method of claim 1, wherein the polysaccharide degradation treatment in the liquid phase pulsed discharge plasma reactor is performed at normal temperature and pressure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201911334349.8A CN112063766A (en) | 2019-12-23 | 2019-12-23 | Polysaccharide degradation method by liquid-phase pulse discharge plasma |
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| CN201911334349.8A CN112063766A (en) | 2019-12-23 | 2019-12-23 | Polysaccharide degradation method by liquid-phase pulse discharge plasma |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1663916A (en) * | 2004-12-16 | 2005-09-07 | 哈尔滨工业大学 | High-pressure pulse discharge catalytic oxidation method for removal of undegradable organic substances in water |
| CN101508790A (en) * | 2009-02-24 | 2009-08-19 | 华南理工大学 | Method for preparing low-molecular weight chitosan by using impulse electric field |
| US20120143121A1 (en) * | 2010-12-07 | 2012-06-07 | Electronics And Telecommunications Research Institute | Chitosan spreading system using low temperature atmospheric pressure plasma |
| CN106834552A (en) * | 2016-12-30 | 2017-06-13 | 大连工业大学 | A kind of polysaccharide is degraded to the low-temperature plasma degradation method of oligosaccharides |
| CN108219020A (en) * | 2016-12-13 | 2018-06-29 | 河南后羿实业集团有限公司 | A kind of extracting method of pachymaran |
-
2019
- 2019-12-23 CN CN201911334349.8A patent/CN112063766A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1663916A (en) * | 2004-12-16 | 2005-09-07 | 哈尔滨工业大学 | High-pressure pulse discharge catalytic oxidation method for removal of undegradable organic substances in water |
| CN101508790A (en) * | 2009-02-24 | 2009-08-19 | 华南理工大学 | Method for preparing low-molecular weight chitosan by using impulse electric field |
| US20120143121A1 (en) * | 2010-12-07 | 2012-06-07 | Electronics And Telecommunications Research Institute | Chitosan spreading system using low temperature atmospheric pressure plasma |
| CN108219020A (en) * | 2016-12-13 | 2018-06-29 | 河南后羿实业集团有限公司 | A kind of extracting method of pachymaran |
| CN106834552A (en) * | 2016-12-30 | 2017-06-13 | 大连工业大学 | A kind of polysaccharide is degraded to the low-temperature plasma degradation method of oligosaccharides |
Non-Patent Citations (4)
| Title |
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| 尹学琼,张岐,于文霞,杨丽春,林强: "Cu(Ⅱ)对壳聚糖的配位控制降解" * |
| 罗文波;唐超;曾新安;: "乙酸均相体系中脉冲电场对壳聚糖降解的影响" * |
| 陈平,郝红元,张岐,葛庆凯,王平红: "壳聚糖铜(Ⅱ)配位与氧化控制降解寡糖的GFC色谱研究" * |
| 马凤鸣;王振宇;: "脉冲放电等离子体降解壳聚糖的研究" * |
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