CN110577657B - Method for improving inoxidizability of noni polysaccharide - Google Patents
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Abstract
The invention discloses a method for improving the oxidation resistance of noni polysaccharide. Firstly, preparing noni polysaccharide into noni polysaccharide solution with the mass concentration of 10-1000 mg/L; and then performing irradiation treatment by using an electron beam of 3-35 kGy at the temperature of 40-90 ℃ to obtain the degraded noni polysaccharide solution. The method realizes high-efficiency degradation of noni polysaccharide, thereby improving the clearance rate of noni polysaccharide on free radicals, and enhancing the effects of noni polysaccharide on resisting aging, tumors and immunity. The method can change the degradation rate by adjusting parameters such as mass concentration, irradiation intensity and temperature, can be controlled in real time, and has the advantages of simple process, low cost, obvious effect, strong operability and great popularization and application value.
Description
Technical Field
The invention belongs to the field of food processing, and particularly relates to a method for improving the oxidation resistance of noni polysaccharide.
Background
The occurrence of aging and many diseases in humans is strongly linked to free radicals. Free radicals are generated in many redox processes in the body. Normally, the generation and elimination of free radicals in the body are in a dynamic balance, and if the balance is broken, excessive oxygen radicals are generated in the body to cause damage to the body, such as causing lipid peroxides to change the structure and function of biological membranes, damaging DNA, causing protein denaturation and enzyme activity loss, and the like, and the damage directly and indirectly causes inflammation, aging, cardiovascular diseases and tumors.
Morinda citrifolia is a plant of Morinda of Rubiaceae, commonly known as Indian Mulberry or Morinda citrifolia, and has great medicinal value. At present, noni fermented juice, freeze-dried powder, capsules, tablet oral liquid and the like exist. A large number of researches show that the noni fermented juice is rich in polysaccharide substances and has obvious anti-aging, anti-tumor and immunity enhancing effects.
Patent 201710109760.X provides a method for obtaining noni polysaccharide, which improves the previous efficiency of obtaining noni polysaccharide from noni, and the content of noni polysaccharide in the obtained extract is higher than that of the extract obtained by the conventional method. And few researches on improving the antioxidant and free radical scavenging capabilities of the noni polysaccharide are carried out.
Disclosure of Invention
The invention aims to provide a method for improving the antioxidant and free radical scavenging capabilities of prepared noni polysaccharide, which utilizes electron beam irradiation treatment to degrade noni polysaccharide solution, optimizes irradiation process conditions such as mass concentration, strength and temperature, and efficiently degrades noni polysaccharide solution, thereby remarkably improving the antioxidant and free radical scavenging capabilities of noni polysaccharide.
The purpose of the invention is realized by the following technical scheme:
a method for improving the inoxidizability of noni polysaccharide comprises the steps of adding water into noni polysaccharide to prepare noni polysaccharide solution with the mass concentration of 10-1000 mg/L; and then irradiating for 40-160 s by using electron beams of 3-35 kGy at the temperature of 40-90 ℃ to obtain the degraded noni polysaccharide solution. The obtained noni polysaccharide has oxidation resistance and free radical scavenging rate remarkably improved.
Wherein, the mass concentration of the noni polysaccharide solution is preferably 70-120 mg/L, and the electron beam irradiation condition is 55-80 ℃, 9-30 kGy and 50-120 s.
More preferably, the mass concentration of the noni polysaccharide solution is 80-120 mg/L, and the electron beam irradiation condition is 60-75 ℃, 12-30 kGy and 60-90 s.
Most preferably, the noni polysaccharide solution has a mass concentration of 90mg/L, and electron beam irradiation conditions of 75 deg.C, 30kGy, 80s.
Preferably, the electron beam is a high frequency linear electron beam.
Preferably, the noni polysaccharide solution is a noni polysaccharide aqueous solution.
More preferably, the water is one of distilled water, purified water, and water for injection. Most preferably distilled water.
In addition, in order to ensure the efficiency of improving the oxidation resistance of the noni polysaccharide, the source and quality of the noni polysaccharide need to satisfy certain conditions. The invention provides a method for preparing noni polysaccharide, which comprises the following steps:
s1, selecting fresh Morinda citrifolia fruits, cleaning, drying and fermenting to obtain noni fermented juice;
s2, carrying out water extraction, concentration and alcohol precipitation on the noni fermented juice to obtain a precipitate, namely noni polysaccharide.
The noni fermented juice obtained by directly and naturally fermenting the fresh noni fruits is selected, other substances contained in the noni fermented juice directly purchased are avoided, and the noni fermented juice fermented by artificial yeast has influence on the degradation of polysaccharide and the effect of degrading the polysaccharide by electron beams due to the adjustment of components such as yeast, pectinase and the like. The method is a hot water extraction method, has simple process, small damage to polysaccharide structure and low cost, is more beneficial to improving the oxidation resistance of the polysaccharide by using electron beam irradiation treatment, is suitable for industrial production of enterprises, and is the preferred method. Compared with general acid-base extraction methods, microwave-assisted extraction methods, enzyme extraction methods, ultrasonic-assisted extraction methods and the like, the acid-base extraction methods can destroy glycosidic bonds of the polysaccharides and influence the structural properties of the polysaccharides; the microwave-assisted extraction method has the advantages of high speed, high efficiency, low solvent consumption, short time and the like, but needs special equipment for assistance, and is high in cost and not suitable for large-scale application; the enzyme extraction method is green and environment-friendly but is not suitable for large-scale application; the ultrasonic-assisted extraction method has short time, high efficiency and easy separation and purification, but needs special instrument auxiliary equipment and has high cost; therefore, a hot water extraction method is selected to study the influence of electron beam irradiation on the degradation of the noni polysaccharide.
Wherein, preferably, the fermentation condition in the step S1 is anaerobic fermentation at 15-20 ℃ for 2-3 months.
Preferably, the water extraction in the step S2 is carried out by hot water extraction at 55-80 ℃ for 2-3 h.
Preferably, the alcohol precipitation is carried out by adding 85-98% ethanol with 3-6 times of volume for precipitation, and the method for remaining precipitate is centrifugation after standing for 10-24 h at 4-10 ℃.
Preferably, the volume ratio of the concentrated solution obtained by concentration in the step S2 to the ethanol used for alcohol precipitation is 1:3 to 6.
Preferably, the volume ratio of the concentrated solution to the ethanol is 1:5, the ethanol concentration is 95%.
Preferably, the step of concentrating and precipitating with ethanol in step S2 is repeated 2 to 4 times to leave the precipitate.
More preferably, the method of step S2 is: hot water leaching, suction filtering, concentrating, alcohol extracting, centrifuging and leaving precipitate to obtain noni polysaccharide.
The noni fermented juice is obtained by natural fermentation of fresh morinda citrifolia fruits, noni polysaccharide is extracted by using ethanol, and the degradation effect of noni polysaccharide solution is improved by using electron beam irradiation. O in the noni polysaccharide solution after electron beam irradiation 2 、H 2 O、CO 2 Equimolecular conversion to a large amount of OH, O, HO 2 Isoactive oxidizing radicals, e aq - Hydration electrons, highly reductive radicals such as H, highly oxidative radicals such as OH, and unsaturated bonds such as benzene ring, hemiacetal reducing group, primary and secondary hydroxyl groups in noni polysaccharide, and aq - the hydrated electrons easily attack the inorganic single element groups in the noni polysaccharide to remove the inorganic single element groups; and after the reductive free radicals such as H and the like are removed, the reductive free radicals are added to the radicals, so that the noni polysaccharide is degraded, and the effect of the noni polysaccharide on removing the free radicals is further improved.
In summary, the present invention provides a method for preparing noni polysaccharide, which comprises preparing noni polysaccharide by the above method, and treating the noni polysaccharide by the above electron beam irradiation method (as shown in fig. 4).
The invention has the following beneficial effects:
the invention adopts electron beam irradiation treatment, and changes the degradation rate by adjusting the conditions of the noni polysaccharide solution such as mass concentration, irradiation intensity, temperature and the like, thereby obtaining better degradation effect and realizing the real-time control of polysaccharide degradation. The noni polysaccharide is noni fermented juice obtained by directly and naturally fermenting fresh morinda citrifolia fruits, and the influence of other substances contained in the directly purchased fermented juice on the measurement of polysaccharide degradation effect influenced by electron beams is avoided.
The noni polysaccharide prepared by the method provided by the invention has 18-30% of clearance rate on free radicals, and the clearance rate can reach more than 90%. The method has the advantages of simple process, low cost, strong operability and great popularization and application value.
Drawings
Fig. 1 is a graph showing the degradation effect of noni polysaccharide solutions of different mass concentrations.
Fig. 2 is a graph showing the degradation effect of noni polysaccharide solutions by different electron beam irradiation intensities.
Fig. 3 is a graph of the degradation effect of noni polysaccharide solutions with different irradiation temperatures.
Fig. 4 is a graph of the degradation effect of noni polysaccharide solutions with different irradiation times.
Fig. 5 is a process flow diagram of the method for preparing noni polysaccharide of the present invention.
Detailed Description
The present invention will be further described with reference to the following specific examples and the accompanying drawings, which are not intended to limit the present invention in any manner. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.
Unless otherwise indicated, reagents and materials used in the present invention are commercially available.
Example 1 preparation of noni polysaccharide solution
The preparation method of the noni polysaccharide solution comprises the following steps:
s1, preparing noni fermented juice
Selecting fresh Morinda citrifolia fruits, cleaning, drying, adding into a fermentation tank, and fermenting at 15 deg.C for 3 months to obtain Morinda citrifolia fermented juice.
S2, preparing noni polysaccharide solution
S21, leaching the obtained noni fermented juice with hot water at 55 ℃ for 2 hours, performing suction filtration and reduced pressure concentration, adding 3 volumes of 85% ethanol into 1 volume of concentrated solution for precipitation, standing for 10 hours at 4 ℃, and centrifuging to obtain precipitate; repeating the steps of adding ethanol for 2 times again for precipitation, standing and centrifuging, and finally combining precipitates to obtain noni polysaccharide;
s22, adding distilled water into the noni polysaccharide obtained in the step S21 to prepare a noni polysaccharide solution with the mass concentration of 10 mg/L.
S3, degrading noni polysaccharide solution by electron beam irradiation
And (4) subjecting the noni polysaccharide solution obtained in the step (S22) to irradiation reaction treatment for 40S by using 3kGy high-frequency linear electron beams at the temperature of 40 ℃ to obtain a degraded noni polysaccharide solution.
Example 2 preparation of a solution of noni polysaccharide
The preparation method of the noni polysaccharide solution comprises the following steps:
s1, preparing noni fermented juice
Selecting fresh Morinda citrifolia fruits, cleaning, drying, adding into a fermentation tank, and fermenting at 15 deg.C for 2 months to obtain Morinda citrifolia fermented juice.
S2, preparing noni polysaccharide solution
S21, leaching the obtained noni fermented juice with hot water at 55 ℃ for 2.5h, carrying out suction filtration, carrying out reduced pressure concentration, adding 4 volumes of 95% ethanol into 1 volume of concentrated solution for precipitation, standing at 5 ℃ for 12h, centrifuging to leave precipitate, adding ethanol again, repeating the operations of adding ethanol for precipitation, standing and centrifuging for 3 times, and finally obtaining combined precipitate which is noni polysaccharide;
s22, adding distilled water into the noni polysaccharide obtained in the step S21 to prepare a noni polysaccharide solution with the mass concentration of 50 mg/L.
S3, degrading noni polysaccharide solution by electron beam irradiation
And (4) irradiating the noni polysaccharide solution obtained in the step (S22) for 50S by using a 12kGy high-frequency linear electron beam at the temperature of 50 ℃ to obtain a degraded noni polysaccharide solution.
Example 3 preparation of a solution of noni polysaccharide
The preparation method of the noni polysaccharide solution comprises the following steps:
s1, preparing noni fermented juice
Selecting fresh Morinda citrifolia fruits, cleaning, drying, adding into a fermentation tank, and fermenting at 18 deg.C for 3 months to obtain Morinda citrifolia fermented juice.
S2, preparing noni polysaccharide solution
S21, leaching the obtained noni fermented juice with hot water at 60 ℃ for 2.5h, carrying out suction filtration, carrying out reduced pressure concentration, adding 5 volume of 95% ethanol into 1 volume of concentrated solution for precipitation, standing for 18h at 7 ℃, centrifuging, then leaving precipitate, adding ethanol again, repeating the operations of adding ethanol for precipitation, standing and centrifuging for 3 times, and finally obtaining noni polysaccharide after merging;
s22, adding distilled water into the noni polysaccharide obtained in the step S21 to prepare a noni polysaccharide solution with the mass concentration of 70 mg/L.
S3, degrading noni polysaccharide solution by electron beam irradiation
And (4) irradiating the noni polysaccharide solution obtained in the step (S22) for 60S by using 9kGy high-frequency linear electron beams at the temperature of 55 ℃ to obtain a degraded noni polysaccharide solution.
Example 4 preparation of noni polysaccharide solution
The preparation method of the noni polysaccharide solution comprises the following steps:
s1, preparing noni fermented juice
Selecting fresh Morinda citrifolia fruits, cleaning, drying, adding into a fermentation tank, and fermenting at 18 deg.C for 2 months to obtain Morinda citrifolia fermented juice.
S2, preparing noni polysaccharide solution
S21, leaching the obtained noni fermented juice with hot water at 70 ℃ for 2.5h, carrying out suction filtration, carrying out reduced pressure concentration, adding 5 volume of 98% ethanol into 1 volume of concentrated solution for precipitation, standing for 20h at 10 ℃, leaving precipitate after centrifugation, adding ethanol again, repeating the operations of adding ethanol for precipitation, standing and centrifuging for 3 times, and finally obtaining noni polysaccharide after merging;
s22, adding distilled water into the noni polysaccharide obtained in the step S21 to prepare a noni polysaccharide solution with the mass concentration of 90 mg/L.
S3, degrading noni polysaccharide solution by electron beam irradiation
And (4) irradiating the noni polysaccharide solution obtained in the step (S22) for 80S by utilizing 30kGy high-frequency linear electron beams at the temperature of 75 ℃ to obtain the degraded noni polysaccharide solution.
Example 5 preparation of a solution of noni polysaccharide
The preparation method of the noni polysaccharide solution comprises the following steps:
s1, preparing noni fermented juice
Selecting fresh Morinda citrifolia fruits, cleaning, drying, adding into a fermentation tank, and fermenting at 18 deg.C for 3 months to obtain Morinda citrifolia fermented juice.
S2, preparing noni polysaccharide solution
S21, leaching the obtained noni fermented juice with hot water at 80 ℃ for 3 hours, carrying out suction filtration and reduced pressure concentration, adding 6-volume 98% ethanol into 1-volume concentrated solution for precipitation, standing at 10 ℃ for 24 hours, centrifuging to obtain precipitate, adding ethanol again, repeating the steps of adding ethanol for precipitation, standing and centrifuging for 4 times, and finally mixing the precipitate to obtain noni polysaccharide;
s22, adding distilled water into the noni polysaccharide obtained in the step S21 to prepare a noni polysaccharide solution with the mass concentration of 80 mg/L.
S3, degrading noni polysaccharide solution by electron beam irradiation
And (5) irradiating the noni polysaccharide solution obtained in the step (S22) for 90S by utilizing an electron beam of 30kGy at the temperature of 60 ℃ to obtain a degraded noni polysaccharide solution.
Example 6 preparation of noni polysaccharide solution
The preparation method of the noni polysaccharide solution comprises the following steps:
s1, preparing noni fermented juice
Selecting fresh Morinda citrifolia fruits, cleaning, drying, adding into a fermentation tank, and fermenting at 20 deg.C for 2 months to obtain Morinda citrifolia fermented juice.
S2, preparing noni polysaccharide solution
S21, leaching the obtained noni fermented juice with hot water at 55 ℃ for 3 hours, carrying out suction filtration and reduced pressure concentration, adding 6-volume 98% ethanol into 1-volume concentrated solution for precipitation, standing at 10 ℃ for 24 hours, centrifuging to obtain precipitate, adding ethanol again, repeating the operations of adding ethanol for precipitation, standing and centrifuging for 4 times, and finally mixing the precipitate to obtain noni polysaccharide;
s22, adding distilled water into the noni polysaccharide obtained in the step S21 to prepare a noni polysaccharide solution with the mass concentration of 120 mg/L.
S3, degrading noni polysaccharide solution by electron beam irradiation
And (5) preparing the noni polysaccharide solution obtained in the step (S22) into 120mg/L, and carrying out irradiation reaction for 120S by utilizing 35kGy electron beams at the temperature of 80 ℃ to obtain the degraded noni polysaccharide solution.
Example 7 preparation of noni polysaccharide solution
The preparation method of the noni polysaccharide solution comprises the following steps:
s1, preparing noni fermented juice
Selecting fresh Morinda citrifolia fruits, cleaning, drying, adding into a fermentation tank, and fermenting at 18 deg.C for 2 months to obtain Morinda citrifolia fermented juice.
S2, preparing noni polysaccharide solution
S21, leaching the obtained noni fermented juice with hot water at 55 ℃ for 3 hours, carrying out suction filtration and reduced pressure concentration, adding 6-volume 98% ethanol into 1-volume concentrated solution for precipitation, standing at 10 ℃ for 24 hours, centrifuging to obtain precipitate, adding ethanol again, repeating the operations of adding ethanol for precipitation, standing and centrifuging for 4 times, and finally mixing the precipitate to obtain noni polysaccharide;
s22, adding distilled water into the noni polysaccharide obtained in the step S21 to prepare a noni polysaccharide solution with the mass concentration of 1000 mg/L.
S3, degrading noni polysaccharide solution by electron beam irradiation
And (4) irradiating the noni polysaccharide solution obtained in the step (S22) for 160S by utilizing 15kGy electron beam at the temperature of 90 ℃ to obtain the degraded noni polysaccharide solution.
EXAMPLE 8 optimization of noni polysaccharide solution concentration for irradiation of Electron Beam
And respectively selecting noni polysaccharide solutions with different mass concentrations of 10-1000 mg/L, and carrying out irradiation treatment under electron beams of 6kGy, 12kGy and 30kGy at the irradiation temperature of 60 ℃ for 80s.
The results are shown in table 1 and fig. 1, respectively. Table 1 shows the effect of irradiation intensity of 12kGy on the radical scavenging rate, fig. 1 is a graph showing the degradation effect of solutions of noni polysaccharide with different mass concentrations, in fig. 1, the x-axis represents the concentration of noni polysaccharide solution, and the y-axis represents the degradation rate of polysaccharide.
TABLE 1
| Noni polysaccharide concentration (mg/L) | 8 | 10 | 30 | 50 | 70 | 80 | 90 | 120 | 1000 |
| ·OH - Clearance (%) | 1.2 | 3.6 | 8.6 | 18.4 | 32.5 | 57.8 | 79.8 | 81.2 | 88.6 |
As can be seen from fig. 1 and table 1, the noni polysaccharide solutions showed different degradation effects depending on the irradiation intensity. When the concentration of noni polysaccharide is low, the degradation effect of electron beams on the concentration of polysaccharide is not obvious. The degradation rate is gradually increased along with the increase of the polysaccharide concentration, but the degradation rate is basically not changed greatly when the polysaccharide concentration reaches more than 90 mg/L. Under the irradiation condition of 12kGy at 90mg/L, the degradation effect approaches a stable state, the degradation rate is 78.2 percent, and the clearance rate of free radicals OH-is 79.8 percent.
This is because, at a constant electron beam intensity, the active radicals having extremely strong oxidizing properties generated in the solution by water do not change much, and the lower the concentration of the polysaccharide solution, the polysaccharide molecules and OH and HO are formed 2 The higher the frequency of free radical collisions, the greater the likelihood of degradation. When the concentration is increased, the active free radicals are not enough to act on all polysaccharide molecules, and the degradation rate is reduced. When the polysaccharide content in the solution is too low, the polysaccharide molecules have less chance of colliding and combining with free radicals, and cannot effectively remove the corresponding free radicals, the free radicals produce minactide or react with other compounds in water, and the degradation rate is correspondingly reduced when the concentration is too high or too low.
EXAMPLE 9 optimization of the intensity of Electron Beam irradiation
Respectively selecting electron beams of 3 kGy-35 kGy, and carrying out irradiation treatment on the noni polysaccharide solutions of 30mg/L, 70mg/L and 120mg/L at the irradiation temperature of 60 ℃ for 80s.
The results are presented in table 2 and fig. 2, respectively. Table 2 shows the effect of the noni polysaccharide solution concentration of 70mg/L on the radical scavenging rate, fig. 2 shows the degradation effect of different electron beam irradiation intensities on the noni polysaccharide solution, the x-axis shows the electron beam irradiation intensity, and the y-axis shows the degradation rate of the polysaccharide in the solution.
TABLE 2
| Irradiation intensity (kGy) | 2 | 3 | 6 | 9 | 12 | 15 | 30 | 35 |
| ·OH - Clearance (%) | 4.5 | 8.6 | 22.4 | 32.4 | 48.6. | 57.6 | 68.6 | 59.5 |
As can be seen from FIG. 2 and Table 2, the degradation rate gradually increased with the increase in the electron beam intensity, but the degradation rate did not change much substantially when the electron beam reached 30kGy, and the radical OH-scavenging rate was 68.6%.
The reason for this is that, under electron beam irradiation, the polysaccharide molecules are gradually decomposed by addition or other chemical reactions of the extremely oxidizing radicals generated from water in the solution with hemiacetal reducing groups, primary and secondary hydroxyl groups, and the degradation rate increases as the irradiation intensity increases.
EXAMPLE 10 optimization of Electron Beam irradiation temperature
Selecting different temperatures of 40-90 ℃, and irradiating 80mg/L noni polysaccharide solution by adopting electron beams of 6kGy, 12kGy and 30kGy for 80s.
The results are presented in table 3 and fig. 3, respectively. Table 3 shows the effect of 12kGy irradiation intensity on the radical clearance, fig. 3 shows the degradation effect of different irradiation temperatures on the noni polysaccharide solution, x-axis shows the irradiation intensity of the electron beam, and y-axis shows the degradation rate of the polysaccharide in the solution.
TABLE 3
| Irradiation temperature (. Degree. C.) | 35 | 40 | 45 | 50 | 55 | 60 | 75 | 90 |
| ·OH - Clearance (%) | 3.4 | 13.4 | 18.8 | 24.2 | 37.6 | 53.6 | 68.7 | 53.6 |
As can be seen from fig. 3 and table 3, the degradation rate gradually increased with the increase of the irradiation temperature, but the degradation rate did not increase significantly when the irradiation temperature was from 40 ℃ to 50 ℃, whereas the degradation rate increased significantly when the irradiation temperature was between 50 ℃ and 75 ℃.
The reason is that under certain electron beam irradiation, the higher the irradiation temperature is, the more easily water molecules in the solution generate OH with strong oxidizability and H free radical with strong reducibility and hydrated electron e aq - A chemical reaction with reducing groups or unsaturated bonds of the polysaccharide molecules occurs, so that the polysaccharide molecules are gradually degraded; however, the temperature is low or too high, which is disadvantageous.
EXAMPLE 11 optimization of Electron Beam irradiation time
Respectively selecting electron beams with the processing time of 40-160 s, and carrying out irradiation treatment on the noni polysaccharide solutions with the concentration of 80mg/L at the irradiation temperature of 60 ℃ and the irradiation treatment on the noni polysaccharide solutions with the processing time of 6kGy, 12kGy and 30 kGy.
The results are presented in table 4 and fig. 4, respectively. Table 4 shows the effect on the radical scavenging rate, fig. 4 is a graph of the degradation effect of noni polysaccharide solution by different electron beam irradiation time, x axis represents the electron beam irradiation time, and y axis represents the degradation rate of polysaccharide in the solution.
TABLE 4
| Irradiation time(s) | 40 | 50 | 60 | 80 | 100 | 120 | 140 | 160 |
| ·OH - Clearance (%) | 45.5 | 60.6 | 78.4 | 80.4 | 78.6 | 77.6 | 78.6 | 79.5 |
As can be seen from FIG. 4 and Table 4, the degradation rate gradually increased with the increase of the electron beam irradiation time, but when the electron beam irradiation time reached 80s, the radical OH-scavenging rate was 80.4%, and the radical OH-scavenging rate was not changed much by continuing to extend the electron beam irradiation time.
The reason for this is that, under electron beam irradiation, the polysaccharide molecules are gradually decomposed by addition or other chemical reactions of the extremely oxidizing radicals generated from water in the solution with hemiacetal reducing groups, primary and secondary hydroxyl groups, and the longer the irradiation time, the greater the degradation rate. But the degradation rate does not change much with time.
Example 12 Multi-factor optimization
Based on the embodiments 7-10, the multi-factor orthogonal experiment is carried out by taking the mass concentration, the irradiation intensity, the irradiation temperature and the irradiation time of the noni polysaccharide solution and taking the clearance rate of the degraded noni polysaccharide product to free radicals as an optimization index.
The result shows that the noni polysaccharide is prepared into noni polysaccharide solution with the mass concentration of 10-1000 mg/L, and then the noni polysaccharide solution is irradiated for 40-160 s by utilizing electron beam with 3-35 kGy at the temperature of 40-90 ℃ to obtain the degraded noni polysaccharide, and the noni polysaccharide has good clearance rate on free radicals.
Preferably, the mass concentration of the noni polysaccharide solution is 70-120 mg/L, and the electron beam irradiation condition is 55-80 ℃, 9-30 kGy and 50-120 s.
More preferably, the mass concentration of the noni polysaccharide solution is 80-120 mg/L, and the electron beam irradiation condition is 60-75 ℃, 12-30 kGy and 60-90 s.
Most preferably, the noni polysaccharide solution has a mass concentration of 90mg/L, and electron beam irradiation conditions of 75 deg.C, 30kGy, 80s. Under the condition, the degraded noni polysaccharide product is p-OH - The clearance rate of free radicals can reach more than 90 percent, and is higher than that of noni polysaccharide p-OH which is not subjected to electron beam irradiation treatment - The clearance rate of free radicals is improved by 30 percent.
It should be understood that the above-described embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.
Claims (8)
1. A method for improving the inoxidizability of noni polysaccharide is characterized in that noni polysaccharide is added with water to prepare noni polysaccharide solution with the mass concentration of 70-120 mg/L; and then irradiating for 50-120 s by using 9-30 kGy high-frequency linear electron beams at the temperature of 55-80 ℃ to obtain a degraded noni polysaccharide solution, wherein the improvement of the inoxidizability of the noni polysaccharide refers to the improvement of the scavenging capacity of the noni polysaccharide to hydroxyl radicals (. OH).
2. The method according to claim 1, wherein the noni polysaccharide solution is an aqueous solution of noni polysaccharide.
3. The method of claim 1, wherein the water is one of distilled water, purified water, and water for injection.
4. The method according to claim 1, wherein said noni polysaccharide is prepared by the following steps:
s1, selecting fresh Morinda citrifolia fruits, cleaning, drying and fermenting to obtain noni fermented juice;
s2, carrying out water extraction, concentration and alcohol precipitation on the noni fermented juice to obtain a precipitate, namely noni polysaccharide.
5. The method according to claim 4, wherein the fermentation in step S1 is carried out under anaerobic fermentation at 15-20 ℃ for 2-3 months.
6. The method as claimed in claim 4, wherein the water extraction in step S2 is performed by leaching with hot water at 55-80 ℃ for 2-3 h.
7. The method as claimed in claim 4, wherein the alcohol precipitation is performed by adding 85-98% ethanol with 3-6 times volume of the ethanol for precipitation, and the method for remaining precipitate is performed by standing at 4-10 ℃ for 10-24 h and then centrifuging.
8. The method according to claim 4, wherein the volume ratio of the concentrated solution obtained by concentration in step S2 to ethanol used for alcohol precipitation is 1:3 to 6.
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