CN1267457C - Method for preparing low molecular weight seaweed polysaccharide sulfate - Google Patents
Method for preparing low molecular weight seaweed polysaccharide sulfate Download PDFInfo
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- CN1267457C CN1267457C CN 200410083685 CN200410083685A CN1267457C CN 1267457 C CN1267457 C CN 1267457C CN 200410083685 CN200410083685 CN 200410083685 CN 200410083685 A CN200410083685 A CN 200410083685A CN 1267457 C CN1267457 C CN 1267457C
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Abstract
The present invention relates to a preparation method of seaweed sulfate polysaccharide which has low molecular weight. Natural seaweed sulfate polysaccharide is used as raw material; ascorbic acid and hydrogen peroxide are added to solution of the seaweed sulfate polysaccharide; the ratio of the amount of substance of the ascorbic acid to the hydrogen peroxide is from 1:0.1 to 10, and the ascorbic acid and the hydrogen peroxide are controlled to be added; an obtained mixture is stirred, and the final concentrations of the amounts of the two kinds of substances are respectively from 0.1 to 50 mmol/L and from 0.1 to 100 mmol/l; the reaction temperature is controlled, and degradation is carried out for 0.5 to 3h at the constant temperature; through dialysis or ultrafiltration, decompression concentration and freeze drying operation, a low molecular weight seaweed sulfate polysaccharide product whose molecular weight is from 4 to 100KDa is prepared. The method has the advantages of high speed of degradation, little reagent dosage and simple technology. At the normal temperature, seaweed sulfate polysaccharide degraded products which have concentrated molecular weight distribution and good uniformity can be obtained. The product has the same structure as the raw material, the content of sulfate radical and total sugar is basically unchanged, the range of the molecular weight can be controlled, the solubility is greatly improved, and the biological activity is obviously strengthened.
Description
Technical field
The present invention relates to the research and development of marine drug, specifically is a kind of preparation method of low molecular weight seaweed polysaccharide sulfate, and it belongs to the marine biotechnology field.
Background technology
Sulfated polysaccharide is the polysaccharide that polysaccharide is formed the one or more hydroxy position generation sulphatings in the unit.In vegitabilia, have only to have natural sulfated polysaccharide in the algae.SULFATED POLYSACCHARIDES FROM SEAWEEDS is the peculiar class polysaccharide of marine alga.SULFATED POLYSACCHARIDES FROM SEAWEEDS has wide biological activity, comprise antiviral, antitumor, to the somatomedin effect, to immunity system effect and anticoagulating active etc.There are report algal polysaccharide sulfate and carrageenin etc. to have anti-HIV-1 cytotoxic activity (CD4
+The T lymphocyte), can suppress HIV-1 to CD4
+The infection of lymphocyte series MT-4.In addition, SULFATED POLYSACCHARIDES FROM SEAWEEDS is also inhibited to other tunicle virus, comprises hsv HSV, cytomegalovirus, influenza A C-type virus C etc.SULFATED POLYSACCHARIDES FROM SEAWEEDS still is that basic fibroblast (bFGF) adheres to the inhibitor that increases to the bFGF dependent cell, can suppress endotheliocyte and form tubular structure, and have anti-mouse RIF-1 tumor growth activity.SULFATED POLYSACCHARIDES FROM SEAWEEDS has two kinds of antitumor mechanism.A kind of by activating macrophage, directly suppress tumor cell proliferation and kill tumor, a kind of is directly to suppress tumor growth and do not influence the healthy tissues growth, thereby is a kind of knurl composition that presses down safely and effectively.SULFATED POLYSACCHARIDES FROM SEAWEEDS also shows the characteristic of some heparitin polysaccharide, by the coenzyme II performance blood coagulation resisting function of heparin.Up to the present, some SULFATED POLYSACCHARIDES FROM SEAWEEDS has shown the potential potential applicability in clinical practice.
The problem that exists is the polysaccharide that natural SULFATED POLYSACCHARIDES FROM SEAWEEDS mostly is macromolecule at present.Because molecular weight is huge, thereby its viscosity is bigger, be difficult for being absorbed by body.High molecular SULFATED POLYSACCHARIDES FROM SEAWEEDS is difficult to by the different barrier of body,, even cytolemma and reach enough Plasma Concentrations perhaps can only rest in the blood vessel and can not reach treated tissue.And low-molecular-weight polysaccharide can more easily pass through these barriers.For example Heparin Oligosaccharides has been found oligosaccharides in blood in the experiment of mouse oral administration, shows that oligosaccharides has diffustivity better in vivo.Studies show that in a large number, different SULFATED POLYSACCHARIDES FROM SEAWEEDS show optimum bioactive molecular weight ranges and inequality, so a kind of good ground degradation method, prepare different low-molecular-weight polysaccharides, to research SULFATED POLYSACCHARIDES FROM SEAWEEDS mechanism of action, improve its existing activity, it is significant to develop its new function.
At present, both at home and abroad to general acid hydrolyzation, alkaline hydrolysis method, enzymolysis process and the supersonic method of adopting of polysaccharide degraded.These methods all have the following disadvantages:
1. acid degradation, under acidic conditions, the difficult control of polysaccharide degradation products molecular weight distribution, sulfate radical content changes greatly.Patent application with the acid degradation algal polysaccharide was disclosed on April 7th, 2004, " marine sulfate polysaccharide 20001 ground manufacture method and application (application number 03138969.4) thereof ".Need to replenish sulfate radical after mentioning acid degradation in this patent with thiosulfonic acid.
2. the alkaline hydrolysis method under alkaline condition, often causes the modification of acidic polysaccharose and coming off of sulfate radical, influences product activity, therefore is not suitable for SULFATED POLYSACCHARIDES FROM SEAWEEDS.
3. enzymolysis process, enzymolysis process are to utilize the specificity Glycosylase to reach the purpose of degraded by a certain glycosidic link in the cracking polysaccharide.Patent application about the enzyme liberating algal polysaccharide was disclosed on June 4th, 2003, " sulfation algae glycan oligosaccharides (application number 02147139.8) ".But, therefore do not have extensive applicability, and the enzyme production cycle is long, loses activity easily, the cost height because the specificity of enzyme is strong.These shortcomings all make this method can't apply at present.
4. the mechanical degradation method comprises methods such as ultrasonic wave and microwave.These two kinds of methods are because the energy consumption height, plant and instrument conditional request height, and quantity of sample handling is little, can't be applied to industrial production at present.
Summary of the invention
At above deficiency, the invention provides a kind of preparation method of low molecular weight seaweed polysaccharide sulfate to the SULFATED POLYSACCHARIDES FROM SEAWEEDS degraded.This preparation method is a kind of oxidation degradation method.Its degradation speed is fast, and reagent dosage is few, and technology is simple, and is with low cost, and products obtained therefrom has the feature structure identical with raw material, and sulfate radical and total sugar content remain unchanged substantially, and molecular weight distribution is concentrated, and molecular weight ranges can be controlled.
Task of the present invention is realized by following technical scheme, has developed a kind of preparation method of low molecular weight seaweed polysaccharide sulfate, and this method is to be raw material with the natural seaweed sulfated polysaccharide, follows these steps to carry out:
(1) take by weighing quantitative SULFATED POLYSACCHARIDES FROM SEAWEEDS, heating is dissolved in the distilled water, stirs and makes rough SULFATED POLYSACCHARIDES FROM SEAWEEDS solution;
(2) in this polysaccharide soln, add xitix and hydrogen peroxide, and be that 1: 0.1～10 ratio control adds by the ratio of both amounts; The xitix in the control adding DeR liquid system and the amount of hydrogen peroxide material after stirring, cause the ultimate density of xitix and hydrogen peroxide amount to be respectively 0.1～50mmol/L and 0.1～100mmol/l;
(3) temperature of control degradation reaction solution system is carried out the constant temperature degraded, and the reaction times is at 0.5～3h;
(4) after reaction finishes, reaction solution is dialysed or ultrafiltration, remove remaining xitix and hydrogen peroxide;
(5), carry out concentrating under reduced pressure, again with the concentrated solution lyophilize with dialyzate or ultrafiltrated in (4); Making molecular weight is the low molecular weight seaweed polysaccharide sulfate product of 4～100KDa;
Described SULFATED POLYSACCHARIDES FROM SEAWEEDS raw material, it is not less than the SULFATED POLYSACCHARIDES FROM SEAWEEDS of 100KDa for molecular weight, and it comprises: laver amylose in the red algae and carrageenin, the hole sea lettuce polysaccharide in the green alga, the algal polysaccharide sulfate in the brown alga.
Described SULFATED POLYSACCHARIDES FROM SEAWEEDS solution, its strength of solution are 0.1～5%.
The xitix in described (2) step control degradation reaction solution system and the concentration of hydrogen peroxide amount, be respectively 5～50mmol/l and 8～100mmol/l, and in the DeR liquid system ratio of control xitix and hydrogen peroxide amount reach 1: 0.3～10, the molecular weight that promptly gets the SULFATED POLYSACCHARIDES FROM SEAWEEDS product is 4～10kD.
The concentration of the xitix in described (2) step control degradation reaction solution system and the amount of hydrogen peroxide material, be respectively 1～30mmol/l and 2～50mmol/1, and in the DeR liquid system ratio of control xitix and hydrogen peroxide amount reach 1: 0.2～10, the molecular weight that promptly gets the SULFATED POLYSACCHARIDES FROM SEAWEEDS product is below the 50kD.
The concentration of the xitix in described (2) step control degradation reaction solution system and the amount of hydrogen peroxide material, be respectively 0.1～30mmol/l and 0.1～50mmol/l, and in the DeR liquid system ratio of control xitix and hydrogen peroxide amount reach 1: 0.1～10, the molecular weight that promptly gets the SULFATED POLYSACCHARIDES FROM SEAWEEDS product is below the 100kD.
The preparation method of above-mentioned low molecular weight seaweed polysaccharide sulfate, the temperature of described (3) step control degradation reaction solution system constant temperature degraded is at 4～80 ℃.
The present invention's advantage compared with the prior art is: because the preparation method of this low molecular weight seaweed polysaccharide sulfate is the hydrogen peroxide synergy system of utilizing the xitix with reductibility and having strong oxidisability, reaction generates suitable active oxyradical, attack the polysaccharide skeleton, thereby ratio between consumption that the molecular weight ranges that makes SULFATED POLYSACCHARIDES FROM SEAWEEDS degraded can be by this xitix of regulation and control and hydrogen peroxide two reagent and two reagent, come the attack degree that suitable active oxyradical is attacked the polysaccharide skeleton is controlled, thereby the polysaccharide molecule weight range after realizing degrading can be controlled.Method of the present invention is with low cost, only uses two kinds of reagent (xitix and hydrogen peroxide) and reagent dosage few; And technology is simple, at normal temperatures, can obtain molecular weight distribution and concentrate the SULFATED POLYSACCHARIDES FROM SEAWEEDS degraded product that homogeneity is good; This product has identical feature structure with raw material, and sulfate radical and total sugar content also keep par with raw material, and the but raising greatly of the solvability of this product, and its biological activity also obviously strengthens.
Accompanying drawing and embodiment
Embodiments of the invention further specify as follows in conjunction with the accompanying drawings:
Fig. 1 is the high performance liquid chromatography of lower molecular weight Porphyra haitanensis polysaccharide.
Fig. 2 is the infared spectrum of lower molecular weight Porphyra haitanensis polysaccharide.
Fig. 3 is the infared spectrum of lower molecular weight yezoensis laver polysaccharide.
Fig. 4 is the high performance liquid chromatography of lower molecular weight yezoensis laver polysaccharide.
Referring to the efficient gel permeation chromatography of Fig. 1, it is the efficient gel permeation chromatography of the Porphyra haitanensis polysaccharide of example 1.This figure shows that the weight-average molecular weight (Mw) of products therefrom is 34537, and number-average molecular weight (Mn) is 13800, and polydispersity index (MWD) is 2.5.
Referring to the infared spectrum of Fig. 2, it is the infared spectrum of the Porphyra haitanensis polysaccharide of example 1.Show among the figure: 3600～3200cm
-1, 3000～2800cm
-1, 1400～1200cm
-1, 1200～1000cm
-1The place has polysaccharide characteristic absorption peak to exist.1224cm
-1Be sulfate radical characteristic absorption peak, 934cm
-1Be 3,6 inner ether semi-lactosi characteristic absorption peaks.
Referring to the infared spectrum of Fig. 3, it is the infared spectrum of the yezoensis laver polysaccharide of example 4.Show among the figure: 3600～3200cm
-1, 3000～2800cm
-1, 1400～1200cm
-1, 1200～1000cm
-1The place has polysaccharide characteristic absorption peak to exist.1226.34cm-1 be sulfate radical characteristic absorption peak, 933cm
-1Be 3,6 inner ether semi-lactosi characteristic absorption peaks.
Referring to the efficient gel permeation chromatography of Fig. 4, it is the efficient gel permeation chromatography of the yezoensis laver polysaccharide of example 4, and this figure shows that the weight-average molecular weight (Mw) of products therefrom is 29695, and number-average molecular weight (Mn) is 11991, and polydispersity index (MWD) is 2.48.
The extraction embodiment of rough SULFATED POLYSACCHARIDES FROM SEAWEEDS of the present invention is as follows:
Described rough SULFATED POLYSACCHARIDES FROM SEAWEEDS raw material, it is the SULFATED POLYSACCHARIDES FROM SEAWEEDS of molecular weight more than 100KDa, it comprises: the laver amylose in the red algae, carrageenin, the sea lettuce polysaccharide in the green alga, the algal polysaccharide sulfate in the brown alga.
The for example extraction of laver amylose: take by weighing the porphyra haitanensis in the quantitative natural air dried red algae, shred; The distilled water that adds 40 times of amounts, 120 ℃ were extracted 3 hours, and with extracting liquid filtering, the distilled water that filter residue adds 40 times of amounts again repeats to extract once, merges filtrate twice; Doing flocculating aids with diatomite filters; Filtrate is dialysed with dialysis tubing, gets dialyzate; Be evaporated to 1/3 volume; Concentrated solution is cooked flocculating aids with diatomite and is filtered; Above-mentioned filtrate joined in 95% the ethanolic soln, the final alcohol concn that makes this solution is 75%; With after the silk cover filtering, again throw out is placed 95% ethanolic soln to dewater the throw out in the above-mentioned ethanol concentrated solution; To pulverize the rough Porphyra haitanensis polysaccharide of the white fiber shape that obtains purifying after the above-mentioned drying precipitate.
The preparation method of low molecular weight seaweed polysaccharide sulfate of the present invention is to be raw material with above-mentioned SULFATED POLYSACCHARIDES FROM SEAWEEDS, follows these steps to carry out:
(1) take by weighing quantitative SULFATED POLYSACCHARIDES FROM SEAWEEDS, heating is dissolved in the distilled water, stirs and makes rough SULFATED POLYSACCHARIDES FROM SEAWEEDS solution;
(2) in this polysaccharide soln, add xitix and hydrogen peroxide again, and be that 1: 0.1～10 ratio control adds by the ratio of both amounts; The xitix in the control adding DeR liquid system and the amount of hydrogen peroxide material after stirring, make the ultimate density of the amount of xitix and hydrogen peroxide material be respectively 0.1～50mmol/L and 0.1～100mmol/l;
(3) constant temperature of control degradation reaction solution system degraded, the reaction times is at 0.5～3h;
(4) after reaction finishes, reaction solution is dialysed or ultrafiltration, remove remaining xitix and hydrogen peroxide;
(5), carry out concentrating under reduced pressure, again with the concentrated solution lyophilize: make shape and be white in color to lurid low molecular weight seaweed polysaccharide sulfate product with dialyzate or ultrafiltrated in (4); Concrete processing condition see Table 1:
Table 1, SULFATED POLYSACCHARIDES FROM SEAWEEDS degradation technique condition and molecular weight of product
The centrifugal 20min of 6000r/m after the degraded of example 1. Porphyra haitanensis polysaccharide, supernatant liquor dialysis 24h removes remaining xitix and hydrogen peroxide; The reconcentration drying gets product afterwards.The molecular weight determination of product: be to use gel permeation chromatography, the differential detector adopts 0.7%Na
2SO4 does moving phase, and 35 degrees centigrade of column temperatures record above-mentioned data.
Example 2--5 SULFATED POLYSACCHARIDES FROM SEAWEEDS degraded example, its polyoses producing method is with example 1 operation, and the amount of polysaccharide concentration, xitix and hydrogen peroxide, reaction times and temperature are as shown in table 1.The centrifugal 20min of 6000r/m after the degraded, supernatant liquor dialysis 24h removes remaining xitix and hydrogen peroxide; The reconcentration drying gets product afterwards.
Example 6--14 SULFATED POLYSACCHARIDES FROM SEAWEEDS degraded example, amount, reaction times and the temperature of its polysaccharide polysaccharide concentration, xitix and hydrogen peroxide are as shown in table 1.Preparation process is with example 1 operation.Reaction soln dialysis 48h removes remaining xitix and hydrogen peroxide after the degraded; The reconcentration drying gets product afterwards.The physical and chemical parameter embodiment of concrete product sees Table 2.
Table 2, the physical and chemical parameter of product of the present invention:
| 7 | 40.8 | 27.54 | 21.96 | 98.5 |
| 8 | 74.41 | 13.68 | 26.75 | 52.4 |
| 9 | 79.11 | 35.67 | 1.46 | 36.4 |
| 10 | 73.10 | 26.68 | 22.97 | 20.8 |
| 11 | 60.07 | 15.36 | 0 | 16.2 |
| 12 | 48.96 | 29.89 | 0 | 29.2 |
| 13 | 50.12 | 29.75 | 0 | 9.6 |
| 14 | 62.54 | 17.66 | 0 | 42.6 |
Those of ordinary skill in the art can understand, and in protection scope of the present invention, makes amendment for the foregoing description, and it all is possible adding and replacing, and it does not all exceed protection scope of the present invention.
Claims (7)
1, a kind of preparation method of low molecular weight seaweed polysaccharide sulfate is characterized in that: this method is that the natural seaweed sulfated polysaccharide that is not less than 100KDa with molecular weight is a raw material, follows these steps to carry out:
(1) take by weighing quantitative SULFATED POLYSACCHARIDES FROM SEAWEEDS, heating is dissolved in the distilled water, stirs and makes SULFATED POLYSACCHARIDES FROM SEAWEEDS solution;
(2) in this polysaccharide soln, add xitix and hydrogen peroxide, and be that 1: 0.1～10 ratio control adds by the ratio of both amounts; The xitix in the control adding DeR liquid system and the amount of hydrogen peroxide material after stirring, cause the ultimate density of xitix and hydrogen peroxide amount to be respectively 0.1～50mmol/L and 0.1～100mmol/l;
(3) temperature of control degradation reaction solution system is carried out the constant temperature degraded, and the reaction times is at 0.5～3h;
(4) after reaction finishes, reaction solution is dialysed or ultrafiltration, remove remaining xitix and hydrogen peroxide;
(5), carry out concentrating under reduced pressure, again with the concentrated solution lyophilize with dialyzate or ultrafiltrated in (4); Make molecular weight at the low molecular weight seaweed polysaccharide sulfate product more than the 4KDa, below the 100KDa.
2, according to the preparation method of the described low molecular weight seaweed polysaccharide sulfate of claim 1, it is characterized in that: described SULFATED POLYSACCHARIDES FROM SEAWEEDS raw material, one of it is selected from: the laver amylose in the red algae, carrageenin, and the sea lettuce polysaccharide in the green alga, in the algal polysaccharide sulfate in the brown alga.
3, according to the preparation method of the described low molecular weight seaweed polysaccharide sulfate of claim 1, it is characterized in that: described SULFATED POLYSACCHARIDES FROM SEAWEEDS solution, its concentration are 0.1～5%.
4, according to the preparation method of the described low molecular weight seaweed polysaccharide sulfate of claim 1, it is characterized in that: the xitix in described (2) step control degradation reaction solution system and the concentration of hydrogen peroxide amount, be respectively 5～50mmol/l and 8～100mmol/l, and in the DeR liquid system ratio of control xitix and hydrogen peroxide amount reach 1: 0.3～10, the molecular weight that promptly gets the SULFATED POLYSACCHARIDES FROM SEAWEEDS product is 4～10KDa.
5, according to the preparation method of the described low molecular weight seaweed polysaccharide sulfate of claim 1, it is characterized in that: the concentration of the xitix in described (2) step control degradation reaction solution system and the amount of hydrogen peroxide material, be respectively 1～30mmol/l and 2～50mmol/l, and in the DeR liquid system ratio of control xitix and hydrogen peroxide amount reach 1: 0.2～10, the molecular weight that promptly gets the SULFATED POLYSACCHARIDES FROM SEAWEEDS product is below the 50KDa.
6, according to the preparation method of the described low molecular weight seaweed polysaccharide sulfate of claim 1, it is characterized in that: the concentration of the xitix in described (2) step control degradation reaction solution system and the amount of hydrogen peroxide material, be respectively 0.1～30mmol/l and 0.1～50mmol/l, and in the DeR liquid system ratio of control xitix and hydrogen peroxide amount reach 1: 0.1～10, the molecular weight that promptly get the SULFATED POLYSACCHARIDES FROM SEAWEEDS product is more than the 4KDa, below the 100KDa.
7, according to the preparation method of each described low molecular weight seaweed polysaccharide sulfate among the claim 1-6, it is characterized in that: the temperature of described (3) step control degradation reaction solution system constant temperature degraded is at 4～80 ℃.
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Families Citing this family (15)
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| CN101028282B (en) * | 2007-03-12 | 2012-05-30 | 北京世纪博康医药科技有限公司 | Use of low-molecular weight phaeophytin polyose sulfate in preparation of medicine for treating kidney disease |
| CN101168570B (en) * | 2007-11-15 | 2010-05-26 | 暨南大学 | Method for degrading kelp polysaccharide sulfate |
| FR2957923B1 (en) * | 2010-03-25 | 2013-06-28 | Greentech | NOVEL HALYMENIA DURVILLEI EXTRACT, PROCESS FOR PREPARING SUCH EXTRACT AND COSMETIC USE THEREOF |
| FR2999084B1 (en) * | 2012-12-11 | 2015-03-13 | Amadeite | EXTRACT OF ALGAE COMPRISING IN PARTICULAR SULFATED AND NON-SULFATED POLYANIONIC POLYSACCHARIDES AND USES THEREOF |
| CN103864946A (en) * | 2014-03-03 | 2014-06-18 | 张忠山 | Porphyra haitanensis polysaccharide positioning sulphating method |
| CN106046187B (en) * | 2016-06-28 | 2018-06-19 | 南京中医药大学 | With free radical cracking product for improving immunocompetent sunset abelmoschus stem or bark leaf polyose and preparation method thereof |
| CN106191914B (en) * | 2016-07-19 | 2018-10-02 | 北京颐方生物科技有限公司 | A method of preparing low-molecular-weight polysaccharide |
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| CN109134682B (en) * | 2018-09-14 | 2021-11-05 | 集美大学 | Red algae oligosaccharide and preparation method and application thereof |
| CN110105461A (en) * | 2019-05-31 | 2019-08-09 | 福建农林大学 | A kind of adjunct antineoplastic compound sea lettuce extract and its technology of preparing |
| CN110713626B (en) * | 2019-10-14 | 2021-06-01 | 福建农林大学 | Preparation method of ulva oligosaccharide compound with auxiliary anti-tumor effect |
| CN111234044B (en) * | 2020-01-19 | 2021-04-27 | 广西中医药大学 | Low-molecular-weight tremella aurantialba glucuronic acid-xylan and preparation method and application thereof |
| CN112480279A (en) * | 2020-12-08 | 2021-03-12 | 浙江工业大学 | Low-molecular-weight sulfated polysaccharide of kelp and preparation and application thereof |
| CN113827610A (en) * | 2021-09-30 | 2021-12-24 | 中国科学院海洋研究所 | Intestinal flora regulator and application thereof |
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