CN112063495B - Nucleic acid extraction device for nucleic acid extraction instrument - Google Patents

Nucleic acid extraction device for nucleic acid extraction instrument Download PDF

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Publication number
CN112063495B
CN112063495B CN202011125589.XA CN202011125589A CN112063495B CN 112063495 B CN112063495 B CN 112063495B CN 202011125589 A CN202011125589 A CN 202011125589A CN 112063495 B CN112063495 B CN 112063495B
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plate
rotating shaft
nucleic acid
symmetrically
gear
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CN112063495A (en
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沈丽姘
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SUZHOU KGENE DIAGNOSTIC Co.,Ltd.
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Suzhou Kgene Diagnostic Co ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA

Abstract

The invention discloses a nucleic acid extraction device for a nucleic acid extractor, which comprises a device body and a door plate, wherein the door plate is provided with a groove; a door plate is rotatably arranged on one side of the device body; the device comprises a device body, wherein a placing device is arranged in the device body, supporting rods are symmetrically and rotatably arranged at the bottom end of the placing device, two groups of supporting rods are arranged in parallel, and the bottom ends of the supporting rods are rotatably arranged in two sides of the bottom end of the device body; the middle part of the bottom end of the supporting rod is provided with a coil spring, the coil spring is sleeved on the rotating shaft, two ends of the coil spring respectively abut against the bottom end of the square groove and the square block, and the two groups of coil springs are symmetrically arranged; the square block is fixedly arranged in the middle of the bottom end of the supporting rod; the bottom ends of the two sides of the placing device are symmetrically and fixedly provided with baffles, and one sides of the baffles, far away from the placing device, are provided with pressing devices. The device of the invention enables the nucleic acid extractor to continuously vibrate through the rotation of the pressing rod and the characteristics of the coil spring, so that the mixed liquid is rapidly and uniformly mixed, the vibration effect is good, and the efficiency is high.

Description

Nucleic acid extraction device for nucleic acid extraction instrument
Technical Field
The invention relates to the technical field of medical equipment, in particular to a nucleic acid extraction device for a nucleic acid extractor.
Background
Biological macromolecular compounds, in which nucleic acids are polymerized from many nucleotides, are one of the most basic substances of life. Nucleic acid is widely present in all animal and plant cells and microorganisms, and nucleic acid in organisms is often combined with protein to form nucleoprotein. Different nucleic acids differ in their chemical composition, nucleotide arrangement order, and the like.
The current nucleic acid extraction reaction system is generally below 1ml, but in many experiments, free nucleic acid needs to be extracted from a large amount of samples, and experimental samples of large-volume reaction systems are needed. And the stirring of the mixed liquid of sample rocks the nucleic acid extraction appearance through the manual work usually and stirs the mixed liquid of sample, and manual stirring can increase the hand labor power, and the effect of manual stirring is relatively poor, and the efficiency of stirring is lower.
Disclosure of Invention
The present invention is directed to a nucleic acid isolation apparatus for a nucleic acid isolation apparatus, which solves the above problems of the prior art.
In order to achieve the purpose, the invention provides the following technical scheme:
a nucleic acid extraction device for a nucleic acid extractor comprises a device body and a door plate; a door plate is rotatably arranged on one side of the device body; a placing device is arranged in the device body; grooves are symmetrically arranged at the bottom end of the placing device; the groove is rotatably provided with support rods through a rotating shaft, and the two groups of support rods are arranged in parallel; the bottom end of the supporting rod is rotatably arranged in the square groove through a rotating shaft; the square grooves are symmetrically arranged in two sides of the bottom end of the device body; a coil spring is arranged in the middle of the bottom end of the supporting rod and sleeved on the rotating shaft; two ends of the coil springs are respectively abutted against the bottom end of the square groove and the square block, and the two groups of coil springs are symmetrically arranged; the square block is fixedly arranged in the middle of the bottom end of the supporting rod; the bottom ends of the two sides of the placing device are symmetrically and fixedly provided with baffles, and one sides of the baffles, far away from the placing device, are provided with pressing devices.
Further, the pressing device comprises a first rotating shaft, a roller and a pressing rod; the first rotating shafts are symmetrically and rotatably arranged in two sides of the lower part of the device body; a roller is fixedly arranged in the middle of the baffle; the rollers are symmetrically and fixedly provided with pressing rods, and the pressing rods on the two groups of rollers are symmetrically arranged; a first gear is fixedly mounted at one end of the first rotating shaft, which is far away from the door panel; the first gear is connected with a rotating device in a transmission fit mode through a first chain.
Further, the rotating device comprises a second rotating shaft, a third gear and a fourth gear; the second rotating shaft and the third rotating shaft are symmetrically and rotatably arranged in one side of the device body away from the door plate; a fourth gear and a third gear are fixedly arranged on the second rotating shaft and the third rotating shaft, and the fourth gears are in transmission engagement; the third gear is in transmission fit connection with a first rotating shaft arranged on the same side through a first chain; the end of the second rotating shaft, which is far away from the third gear, is in transmission connection with a first motor, and the first motor is fixedly installed on the device body.
Further, the placing device comprises a U-shaped plate, a fixing plate, a top plate and a cover; the U-shaped plate is arranged in the lower part of the device body; grooves are symmetrically arranged at the bottom end of the device body; spherical grooves are uniformly distributed in the top ends of the U-shaped plates; a fixing plate is arranged in the top end of the U-shaped plate in a sliding manner; a plurality of groups of fixing grooves are uniformly distributed on the fixing plate; two sides of the fixed plate are arranged in the sliding groove in a sliding manner; the sliding chutes are symmetrically arranged in two sides of the upper part of the U-shaped plate; a positioning groove is formed in one side of the sliding groove, which is far away from the fixed plate, and a fixing device is arranged in the positioning groove in a sliding manner; the fixing plate is fixedly arranged with the U-shaped plate through a fixing device; a top plate is arranged above the U-shaped plate; the top plate is fixedly connected with the U-shaped plate through a lifting device; and a plurality of groups of covers are uniformly and fixedly arranged below the top plate.
Further, the fixing device comprises a positioning pin, a sliding plate, a spring and a pull rod; the positioning pin is arranged in the positioning groove in a sliding manner; one end of the spring, which is far away from the positioning groove, is fixedly arranged on the sliding plate; a spring is arranged on one side of the sliding plate, which is far away from the positioning pin; the sliding plate and the spring are arranged in the circular groove in a sliding manner; the circular grooves are symmetrically arranged in two sides of the fixing plate; a pull rod is fixedly arranged on the top end of the sliding plate; the pull rod is arranged in the moving groove in a sliding manner; the movable grooves are symmetrically arranged in two sides of the top end of the fixed plate and are fixedly communicated with the circular groove.
Further, the lifting device comprises an I-shaped worm, a threaded pipe, a second chain, a fifth gear, a fourth rotating shaft and a second motor; the threaded pipes are symmetrically and rotatably arranged in the two sides of the top plate; the inner side of the threaded pipe is engaged with an I-shaped worm in a transmission manner; the top end of the I-shaped worm is rotatably arranged in the top plate; the bottom end of the I-shaped worm is arranged in the limiting groove in a sliding mode; the limiting grooves are symmetrically arranged in two sides of the U-shaped plate; the outer side of the threaded pipe is connected with a fifth gear in a transmission fit manner through a second chain; the fifth gear is fixedly arranged in the bottom end of the fourth rotating shaft; the fourth rotating shaft is rotatably arranged in the middle of the top plate; the top end of the fourth rotating shaft is in transmission connection with a second motor; the second motor is fixedly arranged on the top plate.
Further, a sensor is fixedly mounted at the top end of the device body; an air heater is fixedly arranged on the outer side of the device body; the output end of the air heater is fixedly communicated with the device body through a guide pipe; the device is characterized in that a controller is fixedly arranged on the outer side of the device body, and the controller is electrically connected with the sensor and the air heater.
Compared with the prior art, the invention has the beneficial effects that:
according to the device, the nucleic acid extractor is enabled to continuously oscillate in the horizontal direction and the vertical direction through the rotation of the pressing rod and the characteristics of the coil spring, so that the mixed liquid in the nucleic acid extractor is rapidly and uniformly mixed, the oscillation effect is good, a plurality of nucleic acid extractors can be oscillated at one time, and the efficiency is high; meanwhile, the sensor and the air heater are arranged, so that the nucleic acid mixed solution is vibrated at a proper constant temperature, and the nucleic acid extraction rate is accelerated.
Drawings
FIG. 1 is a schematic structural diagram of the present invention.
FIG. 2 is a schematic structural diagram of A-A in FIG. 1 according to the present invention.
Fig. 3 is an enlarged structural view of the area B in fig. 1 according to the present invention.
Fig. 4 is an enlarged structural view of the region C in fig. 1 according to the present invention.
Fig. 5 is an enlarged schematic view of region D in fig. 1 according to the present invention.
In the figure: 1-device body, 2-door panel, 3-U-shaped panel, 4-spherical groove, 5-fixing panel, 6-fixing groove, 7-sliding groove, 8-positioning groove, 9-positioning pin, 10-sliding plate, 11-spring, 12-circular groove, 13-pull rod, 14-moving groove, 15-groove, 16-support rod, 17-square block, 18-coil spring, 19-square groove, 20-first rotating shaft, 21-baffle, 22-roller, 23-first gear, 24-first chain, 25-second rotating shaft, 26-third rotating shaft, 27-third gear, 28-fourth gear, 29-first motor, 30-I-shaped worm, 31-test tube cover, 32-limiting groove, 33-a threaded pipe, 34-a second chain, 35-a top plate, 36-a fifth gear, 37-a fourth rotating shaft, 38-a second motor, 39-a sensor, 40-a hot air blower, 41-a guide pipe, 42-a controller and 43-a pressing rod.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
Referring to fig. 1 and 5, in an embodiment of the present invention, a nucleic acid extraction device for a nucleic acid extraction apparatus includes a device body 1 and a door plate 2; a door plate 2 is rotatably arranged on one side of the device body 1; a placing device is arranged in the device body 1; grooves 15 are symmetrically formed in the bottom end of the placing device; the groove 15 is rotatably provided with support rods 16 through a rotating shaft, and the two groups of support rods 16 are arranged in parallel; the bottom end of the supporting rod 16 is rotatably arranged in the square groove 19 through a rotating shaft; the square grooves 19 are symmetrically arranged in two sides of the bottom end of the device body 1; a coil spring 18 is arranged in the middle of the bottom end of the support rod 16, and the coil spring 18 is sleeved on the rotating shaft; two ends of the coil springs 18 are respectively abutted against the bottom end of the square groove 19 and the square block 17, and the two groups of coil springs 18 are symmetrically arranged; the square block 17 is fixedly arranged in the middle of the bottom end of the supporting rod 16; baffle 21 is symmetrically and fixedly installed at the bottom ends of the two sides of the placing device, and a pressing device is arranged on one side, far away from the placing device, of the baffle 21. When the pressing devices arranged on two sides rotate, and the rotating directions of the pressing devices arranged on two sides are opposite, the pressing devices rotate to drive the baffle plate 21 to move in the vertical direction and move along with the horizontal direction, so that the coil springs 18 on one side are in a compressed state, when the pressing devices on two sides are not in contact with the baffle plate 21, the U-shaped plates 3 return to the initial positions under the action of the coil springs 18, because the compression directions of the two groups of coil springs 18 are inconsistent, vibration can be generated, meanwhile, because the rotating directions of the pressing rods 43 arranged on two sides are opposite, the directions of the U-shaped plates 3 driven by the pressing devices arranged on two sides when the pressing devices movably abut against the baffle plate 21 arranged on the same side are opposite, namely, the U-shaped plates 3 are in a vibrated state, namely, the nucleic acid extractor is in continuous vibration, and the nucleic acid extraction rate is accelerated.
Referring to fig. 1 and 2, the pressing device includes a first rotating shaft 20, a roller 22, and a pressing lever 43; the first rotating shaft 20 is symmetrically and rotatably arranged in the two sides of the lower part of the device body 1; a roller 22 is fixedly arranged in the middle of the baffle 21; the rollers 22 are symmetrically and fixedly provided with pressing rods 43, and the pressing rods 43 on the two groups of rollers 22 are symmetrically arranged; a first gear 23 is fixedly mounted at one end of the first rotating shaft 20 far away from the door panel 2; the first gear 23 is connected with a rotating device through a first chain 24 in a transmission fit manner.
Referring to fig. 2, the rotating device includes a second rotating shaft 25, a third rotating shaft 26, a third gear 27 and a fourth gear 28; the second rotating shaft 25 and the third rotating shaft 26 are symmetrically and rotatably arranged in one side of the device body 1 away from the door panel 2; a fourth gear 28 and a third gear 27 are fixedly mounted on the second rotating shaft 25 and the third rotating shaft 26, and the fourth gear 28 is in transmission engagement; the third gear 27 is in transmission fit connection with the first rotating shaft 20 arranged on the same side through the first chain 24; the end of the second rotating shaft 25 far away from the third gear 27 is connected with a first motor 29 in a transmission manner, and the first motor 29 is fixedly installed on the device body 1.
Example 2
Referring to fig. 1 and 4, the placing device includes a U-shaped plate 3, a fixing plate 5, a top plate 35 and a cover 31; the U-shaped plate 3 is arranged in the lower part of the device body 1; grooves 15 are symmetrically formed in the bottom end of the device body 1; spherical grooves 4 are uniformly distributed in the top ends of the U-shaped plates 3; a fixing plate 5 is arranged in the top end of the U-shaped plate 3 in a sliding manner; a plurality of groups of fixing grooves 6 are uniformly distributed on the fixing plate 5; two sides of the fixed plate 5 are arranged in the sliding groove 7 in a sliding manner; the sliding chutes 7 are symmetrically arranged in two sides of the upper part of the U-shaped plate 3; a positioning groove 8 is formed in one side of the sliding groove 7, which is far away from the fixed plate 5, and a fixing device is arranged in the positioning groove 8 in a sliding manner; the fixing plate 5 is fixedly arranged with the U-shaped plate 3 through a fixing device; a top plate 35 is arranged above the U-shaped plate 3; the top plate 35 is fixedly connected with the U-shaped plate 3 through a lifting device; and a plurality of groups of covers 31 are uniformly and fixedly arranged below the top plate 35. 5 can slide in 3 and be convenient for place the nucleic acid extraction appearance, make lid 31 cover the nucleic acid extraction appearance through elevating gear, can avoid getting liquid in the nucleic acid extraction appearance and shaking the in-process spill.
Referring to fig. 4, the fixing device includes a positioning pin 9, a sliding plate 10, a spring 11 and a pull rod 13; the positioning pin 9 is arranged in the positioning groove 8 in a sliding manner; one end of the spring 11, which is far away from the positioning groove 8, is fixedly arranged on the sliding plate 10; a spring 11 is arranged on one side of the sliding plate 10 away from the positioning pin 9; the sliding plate 10 and the spring 11 are arranged in the circular groove 12 in a sliding manner; the circular grooves 12 are symmetrically arranged in two sides of the fixing plate 5; a pull rod 13 is fixedly arranged on the top end of the sliding plate 10; the pull rod 13 is arranged in the moving groove 14 in a sliding manner; the moving grooves 14 are symmetrically arranged in the two sides of the top end of the fixed plate 5, and the moving grooves 14 are fixedly communicated with the circular groove 12.
Referring to fig. 1 and 3, the lifting device includes an i-shaped worm 30, a threaded pipe 33, a second chain 34, a fifth gear 36, a fourth rotating shaft 37 and a second motor 38; the threaded pipes 33 are symmetrically and rotatably arranged in two sides of the top plate 35; the inner side of the threaded pipe 33 is engaged with an I-shaped worm 30 in a transmission manner; the top end of the I-shaped worm 30 is rotatably arranged in the top plate 35; the bottom end of the I-shaped worm 30 is slidably arranged in the limiting groove 32; the limiting grooves 32 are symmetrically arranged in two sides of the U-shaped plate 3; the outer side of the threaded pipe 33 is connected with a fifth gear 36 through a second chain 34 in a transmission fit manner; the fifth gear 36 is fixedly arranged in the bottom end of the fourth rotating shaft 37; the fourth rotating shaft 37 is rotatably installed in the middle of the top plate 35; the top end of the fourth rotating shaft 37 is connected with a second motor 38 in a transmission manner; the second motor 38 is fixedly mounted on the top plate 35.
Example 3
Referring to fig. 1, a sensor 39 is fixedly installed at the top end of the apparatus body 1; an air heater 40 is fixedly arranged on the outer side of the device body 1; the output end of the air heater 40 is fixedly communicated with the device body 1 through a conduit 41; the controller 42 is fixedly installed on the outer side of the device body 1, and the controller 42 is electrically connected with the sensor 39 and the hot air blower 40. In the process of oscillation, the temperature in the U-shaped plate 3 can be monitored in real time through the sensor 39, and the proper temperature in the U-shaped plate 3 is ensured through the hot air blower 40.
The working principle of the invention is as follows:
when nucleic acid is extracted, the pull rod 13 is pulled to move towards one side far away from the positioning groove 8, so that the positioning pin 9 slides out of the positioning groove 8, the fixing plate 5 moves downwards, the nucleic acid extractor filled with nucleic acid penetrates through the fixing groove 6 to be fixedly placed, after the placing is finished, the fixing plate 5 is pulled to move upwards, and the fixing plate 5 slides into the positioning pin 9 through the positioning groove 8 to be fixed; and starting a second motor 38, wherein the second motor 38 rotates to drive a fourth rotating shaft 37 and a fifth gear 36 to rotate, the fifth gear 36 rotates to drive a threaded pipe 33 to rotate through a second chain 34, the threaded pipe 33 rotates to drive an I-shaped worm 30 to rotate, so that the cover 31 and the top plate 35 move downwards, and the top plate 35 covers the nucleic acid extractor; when the first motor 29 is started, the first motor 29 rotates to drive the second rotating shaft 25, the third gear 27 on the second rotating shaft 25 and the fourth gear 28 on the second rotating shaft 25 to rotate, the fourth gear 28 on the second rotating shaft 25 drives the fourth gear 28 on the third rotating shaft 26 to rotate in opposite directions, i.e. two sets of third gears 27 rotate in opposite directions, the third gear 27 drives the first gear 23 to rotate through the first chain 24, the first gear 23 rotates, i.e. the first rotating shaft 20, the roller 22 and the pressing rod 43 rotate, and the pressing rods 43 arranged on both sides rotate in opposite directions, the pressing rod 43 rotates to drive the baffle plate 21 to move in the vertical direction and move in the horizontal direction, so that the coil spring 18 on one side is in a compressed state, when the pressing rods 43 on both sides are not in contact with the baffle plate 21, the U-shaped plate 3 returns to the initial position under the action of the coil spring 18, because the compression directions of the two sets of coil springs 18 are not consistent, will vibrate, simultaneously because the pressing rods 43 that both sides set up rotate in opposite directions, make the pressing rods 43 that both sides set up lie in that the homonymy sets up that the baffle 21 activity is balanced the U-shaped board 3 that takes moves the opposite direction, make the U-shaped board 3 be in the state of vibrating, make the nucleic acid extraction appearance be in and ceaselessly vibrate for the speed of nucleic acid extraction is accelerated. In the process of oscillation, the temperature in the U-shaped plate 3 can be monitored in real time through the sensor 39, and the proper temperature in the U-shaped plate 3 is ensured through the hot air blower 40.
The standard parts used in the invention can be purchased from the market, the special-shaped parts can be customized according to the description of the specification and the accompanying drawings, the specific connection mode of each part adopts conventional means such as bolts, rivets, welding and the like mature in the prior art, the machines, the parts and equipment adopt conventional models in the prior art, and the circuit connection adopts the conventional connection mode in the prior art, so that the detailed description is omitted.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims (5)

1. A nucleic acid extraction device for a nucleic acid extractor comprises a device body (1) and a door plate (2); a door plate (2) is rotatably arranged on one side of the device body (1); the device is characterized in that a placing device is arranged in the device body (1); grooves (15) are symmetrically arranged at the bottom end of the placing device; the supporting rods (16) are rotatably arranged in the grooves (15) through rotating shafts, and the two groups of supporting rods (16) are arranged in parallel; the bottom end of the supporting rod (16) is rotatably arranged in the square groove (19) through a rotating shaft; the square grooves (19) are symmetrically arranged in two sides of the bottom end of the device body (1); a coil spring (18) is arranged in the middle of the bottom end of the supporting rod (16), and the coil spring (18) is sleeved on the rotating shaft; two ends of the coil springs (18) are respectively abutted against the bottom end of the square groove (19) and the square block (17), and the two groups of coil springs (18) are symmetrically arranged; the square block (17) is fixedly arranged in the middle of the bottom end of the supporting rod (16); the bottom ends of the two sides of the placing device are symmetrically and fixedly provided with baffle plates (21), and one side of each baffle plate (21) far away from the placing device is provided with a pressing device;
the pressing device comprises a first rotating shaft (20), a roller (22) and a pressing rod (43); the first rotating shafts (20) are symmetrically and rotatably arranged in two sides of the lower part of the device body (1); a roller (22) is fixedly arranged in the middle of the baffle (21); the rollers (22) are symmetrically and fixedly provided with pressing rods (43), and the pressing rods (43) on the two groups of rollers (22) are symmetrically arranged; a first gear (23) is fixedly mounted at one end of the first rotating shaft (20) far away from the door panel (2); the first gear (23) is connected with a rotating device in a transmission fit manner through a first chain (24); the rotating device comprises a second rotating shaft (25), a third rotating shaft (26), a third gear (27) and a fourth gear (28); the second rotating shaft (25) and the third rotating shaft (26) are symmetrically and rotatably arranged in one side of the device body (1) far away from the door panel (2); a fourth gear (28) and a third gear (27) are fixedly arranged on the second rotating shaft (25) and the third rotating shaft (26), and the fourth gears (28) are in transmission engagement; the third gear (27) is in transmission fit connection with the first rotating shaft (20) arranged on the same side through a first chain (24); and the end of the second rotating shaft (25) far away from the third gear (27) is in transmission connection with a first motor (29), and the first motor (29) is fixedly installed on the device body (1).
2. The nucleic acid extraction device for a nucleic acid extractor according to claim 1, wherein the placement device comprises a U-shaped plate (3), a fixing plate (5), a top plate (35), and a lid (31); the U-shaped plate (3) is arranged in the lower part of the device body (1); grooves (15) are symmetrically formed in the bottom end of the device body (1); spherical grooves (4) are uniformly distributed in the top ends of the U-shaped plates (3); a fixing plate (5) is arranged in the top end of the U-shaped plate (3) in a sliding manner; a plurality of groups of fixing grooves (6) are uniformly distributed on the fixing plate (5); two sides of the fixed plate (5) are arranged in the sliding groove (7) in a sliding manner; the sliding chutes (7) are symmetrically arranged in two sides of the upper part of the U-shaped plate (3); a positioning groove (8) is formed in one side, away from the fixing plate (5), of the sliding groove (7), and a fixing device is arranged in the positioning groove (8) in a sliding mode; the fixing plate (5) is fixedly installed with the U-shaped plate (3) through a fixing device; a top plate (35) is arranged above the U-shaped plate (3); the top plate (35) is fixedly connected with the U-shaped plate (3) through a lifting device; and a plurality of groups of covers (31) are uniformly and fixedly arranged below the top plate (35).
3. The nucleic acid isolation apparatus for a nucleic acid isolation instrument according to claim 1, wherein a sensor (39) is fixedly mounted on the top end of the apparatus body (1); an air heater (40) is fixedly arranged on the outer side of the device body (1); the output end of the hot air blower (40) is fixedly communicated with the device body (1) through a guide pipe (41); the device is characterized in that a controller (42) is fixedly mounted on the outer side of the device body (1), and the controller (42) is electrically connected with the sensor (39) and the air heater (40).
4. The nucleic acid extraction device for the nucleic acid extractor according to claim 2, wherein the fixing means comprises a positioning pin (9), a slide plate (10), a spring (11) and a pull rod (13); the positioning pin (9) is arranged in the positioning groove (8) in a sliding manner; one end of the spring (11) far away from the positioning groove (8) is fixedly arranged on the sliding plate (10); a spring (11) is arranged on one side, away from the positioning pin (9), of the sliding plate (10); the sliding plate (10) and the spring (11) are arranged in the circular groove (12) in a sliding manner; the circular grooves (12) are symmetrically arranged in two sides of the fixing plate (5); a pull rod (13) is fixedly arranged on the top end of the sliding plate (10); the pull rod (13) is arranged in the moving groove (14) in a sliding manner; the moving grooves (14) are symmetrically arranged in two sides of the top end of the fixed plate (5), and the moving grooves (14) are fixedly communicated with the circular groove (12).
5. The nucleic acid extraction device for the nucleic acid extraction instrument according to claim 2, wherein the lifting device comprises an I-shaped worm (30), a threaded pipe (33), a second chain (34), a fifth gear (36), a fourth rotating shaft (37) and a second motor (38); the threaded pipes (33) are symmetrically and rotatably arranged in two sides of the top plate (35); the inner side of the threaded pipe (33) is engaged with an I-shaped worm (30) in a transmission manner; the top end of the I-shaped worm (30) is rotatably arranged in the top plate (35); the bottom end of the I-shaped worm (30) is arranged in the limiting groove (32) in a sliding manner; the limiting grooves (32) are symmetrically arranged in two sides of the U-shaped plate (3); the outer side of the threaded pipe (33) is connected with a fifth gear (36) through a second chain (34) in a transmission fit manner; the fifth gear (36) is fixedly arranged in the bottom end of the fourth rotating shaft (37); the fourth rotating shaft (37) is rotatably arranged in the middle of the top plate (35); the top end of the fourth rotating shaft (37) is in transmission connection with a second motor (38); the second motor (38) is fixedly arranged on the top plate (35).
CN202011125589.XA 2020-10-20 2020-10-20 Nucleic acid extraction device for nucleic acid extraction instrument Active CN112063495B (en)

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