CN112057463A

CN112057463A - Application of cowherb seed flavonoid glycoside in preventing alopecia and promoting hair growth

Info

Publication number: CN112057463A
Application number: CN202011096581.5A
Authority: CN
Inventors: 吴亮; 蔡溪云弋; 蔡磊; 冯陈杰; 吴舒婷; 董昊; 郝园园; 陈佳敏; 刘宙; 刘玉琳; 朱俊霖; 徐佳怡; 曹毅眀; 吴晓明
Original assignee: China Pharmaceutical University
Current assignee: China Pharmaceutical University
Priority date: 2020-10-14
Filing date: 2020-10-14
Publication date: 2020-12-11

Abstract

The invention provides an application of cowherb seed flavonoid glycoside and physiologically acceptable salt or solvate thereof in preparing a skin application composition and an application of the skin application composition in pharmaceutical preparations, cosmetics, nursing products and cosmetics. The composition for skin application and the further applied preparation can comprehensively and effectively aim at various reasons of alopecia, so that the composition is more helpful for balancing the metabolic condition of a demand object, adjusting the mental state of the demand object to achieve better treatment and nursing synergistic effect, and the active ingredient of the cowherb seed flavonoid glycoside has high safety and small toxic and side effects, so that the composition has wide application prospect in the fields of pharmaceutical preparations, cosmetics, nursing products, cosmetics and the like.

Description

Application of cowherb seed flavonoid glycoside in preventing alopecia and promoting hair growth

Technical Field

The invention belongs to the field of compounds and preparations thereof, and particularly relates to application of cowherb seed flavonoid glycoside in preparing a preparation for preventing alopecia and promoting hair growth, and further application in the fields of medicines, cosmetics, nursing products, cosmetics and the like.

Background

Alopecia refers to the phenomenon of hair loss. The normal hair loss is the hair in the catagen and telogen phases, and the normal amount of hair can be maintained because the hair entering the catagen phase and the hair newly entering the anagen phase are constantly in dynamic balance. Pathological alopecia refers to abnormal or excessive hair loss caused by excessive stress, or night-out, endocrine disturbance, excessive sebum excretion, etc. In recent years, the alopecia population in China is on the rise, and on average, one person in 6 people has alopecia, wherein the proportion of people between 20 years old and 40 years old is large. The investigation of the alopecia population released by the national Weijian Commission shows that the number of alopecia population in China exceeds 2.5 hundred million.

Relatively few preparations have been used to treat alopecia. Currently, the drugs approved by the U.S. Food and Drug Administration (FDA) for the treatment of alopecia are minoxidil and finasteride. Finasteride is a specific inhibitor of the intracellular enzyme, type II 5 a-reductase, in the metabolism of testosterone to the stronger dihydrotestosterone. Finasteride inhibits prostate hyperplasia, ameliorates benign prostate hyperplasia, and ameliorates androgenic alopecia by reducing dihydrotestosterone levels in blood and prostate tissue. But finasteride still has the side effects of erectile dysfunction, sexual hypofunction, male breast enlargement and the like. And the minoxidil can improve blood circulation, open a potassium ion channel and promote hair growth. But also causes itching, rash and even arrhythmia.

Dried mature seeds of Vaccaria segetalis (Neck.) Garcke of Dianthus japonicus of Dianthaceae. The semen Vaccariae mainly contains triterpenoid saponin, flavonoid glycoside, cyclic peptide, lipoid, fatty acid, monosaccharide, etc. The cowherb seed flavonoid glycoside (Vaccarin) is a flavonoid triglycoside extracted from cowherb seed, and is one of flavonoid glycoside compounds. The flavonoid compound shows certain activity in the aspects of resisting inflammation of body parts, regulating lipid metabolism and the like, can effectively reduce lipid accumulated in human organ tissues, reduce inflammation, has certain antioxidant stress, and has pharmacological actions of regulating islet balance, improving endothelial injury, promoting angiogenesis, inhibiting hyperglycemia, hypertension, hyperlipidemia and the like.

As described above, in order to treat alopecia, it is highly desirable to find an active substance that can promote the natural growth of hair, reduce alopecia, be safe, stable, non-irritating, and fully satisfy the requirements for caring, treating, and beautifying effects, for various reasons caused by alopecia. The cowherb seed flavonoid glycoside has good effects in treating alopecia and promoting hair growth.

Disclosure of Invention

In order to solve the problems of the prior art, the present invention provides the use of cowherb seed flavonoid glycoside and physiologically acceptable salts or solvates thereof for the preparation of a composition for dermal application.

According to an embodiment of the invention, the skin application composition is a composition having a synergistic effect of one or more of an antiandrogen, a hair growth stimulating, an anti-sebum effect; in some embodiments, the skin application composition is an antiandrogen composition; in some embodiments, the skin application composition is a composition for stimulating hair growth; in some embodiments, the skin application composition is an anti-sebum composition.

According to an embodiment of the invention, the skin comprises tissue covering the surface of the human or animal body, further comprising tissue covering the surface of the face or body, as well as the scalp and hair; the hair encompasses body hair and head hair (e.g., few hairs, "hairs" in alopecia further encompass body hair and head hair); preferably, the skin is the scalp; preferably the hair is hair.

According to an embodiment of the invention, the subject to which the skin-applied composition is applied includes humans and animals.

According to embodiments of the present invention, the stimulating hair growth includes stimulating the production of new hair, as well as promoting the growth of existing hair in a healthy state (reducing the risk of hair loss);

according to embodiments of the present invention, the skin-applied composition can inhibit androgens, stimulate the proliferation of hair follicle papilla cells and hair growth of hair follicle cells, while controlling sebum hypersecretion.

According to an embodiment of the present invention, the anti-sebum composition may be applied to condition or improve skin problems caused by excessive secretion of sebaceous glands, such as follicular dilatation, acne, seborrheic dermatitis, and the like.

According to an embodiment of the invention, the dermally administrable composition is used for the prevention and/or treatment of hair loss in a patient after chemotherapy.

According to an embodiment of the invention, the dermally administrable composition is for use in the prevention and/or treatment of androgenic alopecia.

According to an embodiment of the invention, the dermally administrable composition is used for preventing and/or treating alopecia resulting from autoimmune disease alopecia areata.

According to embodiments of the present invention, in some embodiments, the composition comprises cowherb seed flavonoid glycoside and physiologically acceptable salts or solvates thereof as the only physiologically active ingredient; in other embodiments, the composition comprises other physiologically active ingredients in addition to the vaccaria flavonoid glycoside and physiologically acceptable salts or solvates thereof as an active ingredient.

The invention further provides the application of the skin application composition in pharmaceutical preparations, cosmetics, nursing products and cosmetics.

According to an embodiment of the invention, the pharmaceutical preparation is applied for preventing, caring and treating oligotrichia and alopecia, promoting hair health and growth, and inhibiting sebum secretion.

The pharmaceutical preparation can be applied topically through skin, and the dosage form of the preparation is selected from cream, patch, ointment, cream preparation, gel, spray, etc., and can also be orally taken (i.e. oral preparation), and the oral preparation comprises tablet, granule, capsule, oral liquid, pill, suspension, dripping pill, etc. The pharmaceutical formulation further comprises a physiologically acceptable carrier (e.g. a material with biocompatibility), for example optionally comprising surfactants, excipients, humectants, emulsification promoters, suspending agents, salts or buffers for regulating the osmotic pressure, colorants, fragrances, stabilizers, bactericides, preservatives or other conventional supplements. According to an embodiment of the present invention, the pharmaceutical formulation may include the cowherb seed flavonoid glycoside and the physiologically acceptable salt or solvate thereof in an amount of 0.01 wt% to 20 wt% based on the total weight of the formulation. Preferably, the cowherb seed flavonoid glycoside and the physiologically acceptable salt or solvate thereof account for 3 to 7 weight percent of the total weight of the preparation.

According to an embodiment of the present invention, the cosmetic, care or beauty product may be selected from a hair shampoo, a hair conditioner, a hair care, a hair conditioner essence, a hair oil, a hair conditioner, a hair lotion, a scalp hair tonic, a hair essence, a scalp cream, a hair conditioner, a hair spray, a hair pack, a eyebrow care, and the like.

Advantageous effects

The inventor finds that the vaccaria flavonoid glycoside can promote the growth speed of naturally growing hair, increase the hair length and the hair weight, care hair follicles, condition or improve excessive secretion of sebaceous glands, particularly can prevent and treat alopecia caused by chemotherapeutic drugs, effectively treat androgen alopecia and alopecia areata, and can more effectively increase the growth weight, the length and the like of newly added hair or residual hair after alopecia compared with a marketed drug minoxidil. The physiological activity of the vaccaria flavonoid glycoside can comprehensively and effectively aim at various reasons of alopecia, so that the physiological activity of the vaccaria flavonoid glycoside is more beneficial to balancing the metabolic condition of a demand object and adjusting the mental state of the demand object to achieve better treatment and nursing synergistic effect, and the active ingredients have high safety and small toxic and side effects, so the physiological activity has wide application prospect in the fields of medical nursing and the like.

Drawings

FIG. 1 is a histogram of the hair length of cowherb seed flavonoid glycoside on a naturally growing model mouse;

FIG. 2 is a histogram of the hair weight of cowherb seed flavonoid glycoside on a naturally growing model mouse;

FIG. 3 is a bar graph of the hair growth score of cowherb seed flavonoid glycoside on post-chemotherapy baldness model mice;

FIG. 4 is a histogram of hair length of cowherb seed flavonoid glycosides on post-chemotherapy baldness model mice;

FIG. 5 is a histogram of the hair weight of cowherb seed flavonoid glycosides on post-chemotherapy baldness model mice;

FIG. 6 is a line graph of the hair growth score of cowherb seed flavonoid glycoside on an AGA model mouse;

FIG. 7 is a histogram of the hair length of cowherb seed flavonoid glycosides on AGA model mice;

FIG. 8 is a histogram of the hair weight of cowherb seed flavonoid glycosides on AGA model mice.

Detailed Description

The technical solution of the present invention will be further described in detail with reference to specific embodiments. It is to be understood that the following examples are only illustrative and explanatory of the present invention and should not be construed as limiting the scope of the present invention. All the technologies realized based on the above-mentioned contents of the present invention are covered in the protection scope of the present invention.

Unless otherwise indicated, the raw materials and reagents used in the following examples are all commercially available products or can be prepared by known methods.

In the examples, "%" is used as a mass percentage unless otherwise specified.

Example 1: experimental study for evaluating curative effect of cowherb seed flavonoid glycoside on C57BL/6 mouse natural hair growth model

1. Laboratory animal

The experimental animals are male C57BL/J6, 8 weeks old and 18-20g in weight, and are purchased from the comparative medical center of Yangzhou university. The C57BL/6 mouse is bred under normal conditions, the temperature is 25 +/-2 ℃, the humidity is 50% -70%, the illumination and the darkness are carried out alternately for 12h respectively, the mouse can eat and drink water freely, the corn cob padding is replaced every two days, and the experiment is started after the mouse adapts to the breeding environment for 7 days.

2. Drug configuration

4% chloral hydrate: 0.4g chloral hydrate is dissolved in 10ml of normal saline, and is subjected to vortex centrifugation to be completely dissolved to prepare 4% chloral hydrate solution.

2.5% (O/W) of the external cream of the cowherb seed flavonoid glycoside is prepared from the following raw material medicaments:

175 parts of cowherb seed flavonoid glycoside, 1000 parts of stearic acid, 200 parts of glyceryl stearate, 200 parts of beeswax, 200 parts of palm oil, 600 parts of butanediol, 200 parts of ethanol, 4800 parts of water, 3 parts of carbomer and 2 parts of triethanolamine. The external cream of the invention is prepared according to the raw material components:

(1) weighing stearic acid, glyceryl stearate, beeswax and palm oil according to a certain proportion, heating in a water bath to be molten, filtering, and preserving at a constant temperature of 70-80 ℃;

(2) weighing butanediol, ethanol, water, carbomer and triethanolamine according to a certain proportion, adding into the solution, heating in water bath, stirring, and emulsifying for 10-20 min;

(3) adding the weighed raw material of the cowherb seed flavonoid glycoside, stirring the mixture while adding the raw material until the solution is condensed into cream to obtain the external emulsifiable paste of the cowherb seed flavonoid glycoside with the concentration of 2.5 percent (O/W)

The cowherb seed flavonoid glycoside oral preparation comprises the following components: the dosage is 100mg/kg, the drug is dissolved by 0.5 percent CMC-Na before use, the solution is centrifuged by vortex, and the mice are orally administrated with gastric lavage after being completely suspended.

3. Natural alopecia model establishment

After a mouse is weighed, 4% chloral hydrate is intraperitoneally injected according to a dose of 10l/g, after the mouse is anesthetized, an electric shaver carefully shaves off all back hairs of the mouse to avoid wounds, depilatory wax paper with the size of 5.25 multiplied by 2.5cm is cut, a palm is warm for 1min and then torn, the electric shaver is attached to the center of the back of the mouse along the growth direction of the mouse hairs, the palm is continuously warm for 1min, the tail of the mouse is fixed, the wax paper is quickly torn in the reverse hair growth direction, then the electric shaver is wiped by using skin moistening paper, all hairs in the terminal phase are pulled out, the development of highly synchronous hair follicles in the growth phase is induced, and the hair follicles are not different from the spontaneously formed hair follicles in shape.

24C 57BL/6 mice were randomly assigned to a model control group (model), minoxidil tincture group (Mino), cowherb seed flavonoid glycoside ointment group (Vaccarin ointment), and cowherb seed flavonoid glycoside oral group (Vaccarin oral), each group consisting of 6 mice. The administration was performed 1 time per day for 13 days in the depilated area of the back of the mouse from day 2 after the depilation. The concentration of commercially available minoxidil tincture was 5%, and the solution was diluted to 2.5% by adding an equal volume of anhydrous ethanol, and the dose was 200. mu.l per one. Control group was given a blank cream without drug; the concentration of the cowherb seed flavonoid glycoside group is 2.5%, and about 0.2g of cotton swab dipping paste is used for applying to the depilation region in each administration.

4. Experimental for evaluating curative effect of cowherb seed flavonoid glycoside on natural growth of hair of unhaired mouse

On the 14 th day after administration, the mice were injected with 10l/g 4% chloral hydrate intraperitoneally, when the mice were anesthetized stably, the mice were carefully nipped with forceps to take hair from 10 fixed sites on the back of the mice, about 10 hairs were taken each time, the mice were placed on clean white paper, the length was measured with a vernier caliper, the longest value was selected as the recorded value, and the whole measurement process was repeated 2 times.

As a result, as shown in fig. 1, the vaccaria flavonoid glycoside ointment group significantly increased hair length compared to the blank cream control group, which had a statistical significance (. about.p <0.01), and significantly promoted hair growth. The change in the oral group of vaccaria flavonol glycosides was slightly elevated compared to the model (P. 0.1278), similar to minoxidil. The cowherb seed flavonoid glycoside ointment group was also able to significantly increase hair length compared to the positive drug minoxidil (. about.p < 0.01).

After the hair length measurement is finished, the hair in the molding area is carefully shaved off by using an animal shaver, the hair is weighed by using a one-hundred-ten-thousand balance after being collected and stored by using weighing paper, and the hair weight value is recorded.

The results are shown in fig. 2, the vaccaria flavonoid glycoside soft cream group significantly increased hair weight compared to the blank cream control group, which had statistical significance (. about.p <0.01), indicating that vaccaria flavonoid glycoside can promote hair growth. The cowherb seed flavonoid glycoside ointment group also increased hair weight compared to the positive drug minoxidil (P ═ 0.082), but was not statistically significant. The oral administration group of vaccaria flavonoid glycoside can also increase hair weight (P < 0.05).

It can be seen that the cowherb seed flavonoid glycoside has obvious curative effect in the process of promoting the natural hair growth of mice, and particularly, the ointment preparation is better than the positive drug minoxidil in the aspect of hair weight. In the process of natural hair growth, the cowherb seed flavonoid glycoside can better promote the growth of the hair of the mouse.

Example 2: experimental study for evaluating curative effect of cowherb seed flavonoid glycoside on C57BL/6 mouse chemotherapy alopecia model

1. Laboratory animal

The experimental animals are male C57BL/6, 8-week-old, 18-20g in weight, and purchased from the compared medical center of Yangzhou university. The C57BL/6 mouse is bred under normal conditions, the temperature is 25 +/-2 ℃, the humidity is 50% -70%, the illumination and the darkness are carried out alternately for 12h respectively, the mouse can eat and drink water freely, the corn cob padding is replaced every two days, and the experiment is started after the mouse adapts to the breeding environment for 7 days.

2. Drug configuration

The 4% chloral hydrate was prepared as above.

The ointment of the cowherb seed flavonoid glycoside is prepared as above.

The preparation method of the oral suspension of the cowherb seed flavonoid glycoside is the same as the preparation method.

Cyclophosphamide for injection: 0.2g cyclophosphamide is dissolved in 20ml physiological saline, and the solution is prepared into 10mg/ml solution after being completely dissolved by vortex centrifugation.

3. Establishment of chemotherapy alopecia model

On the 9 th day after depilation, the hair follicles in the depilation region entered the early stage of growth phase VI, and cyclophosphamide solution with the concentration of 80mg/kg and 10mg/ml was intraperitoneally injected in a single dose to induce the growth inhibition of the hair follicles in all the growth phases and thus the hair loss after chemotherapy. The blank mice were injected with the same dose of saline.

4. Therapeutic experiment on depilated mice

30 depilated mice were randomized into 5 groups of 6 mice each: blank control group, model group, cowherb seed flavonoid glycoside ointment group, cowherb seed flavonoid glycoside oral group and minoxidil group. Blank cream without drug was given to blank control and model groups; the administration concentration of the cowherb seed flavonoid glycoside group is 2.5%, and about 0.2g of cotton swab dipping paste is used for applying to a depilation area in each administration; the concentration of commercially available minoxidil tincture was 5%, and the solution was diluted to 2.5% by adding an equal volume of anhydrous ethanol, and the dose was 200. mu.l per one. The administration was continued 1 time per day from day 2 after depilation for a total of 13 days to day 5 after cyclophosphamide molding.

5. Experimental for evaluating curative effect of cowherb seed flavonoid glycoside on hair chemotherapy alopecia of depilated mice

And 6 days after the cyclophosphamide is modeled, injecting 10l/g 4% chloral hydrate into the abdominal cavity of the mouse, photographing under standard conditions to record the back skin condition and the hair growth condition of each group of mice and scoring and counting when the mouse is anesthetized and stabilized.

Grading standard: the remaining hair area is a proportion of the total area of the epilation area.

The results are shown in fig. 3, and the blank group is significantly different from the model group (P < 0.001), indicating that the chemotherapy alopecia model successfully causes the mice to have fewer and shorter hairs, which is visible to the naked eye. Compared with the model group, the cowherb seed flavonoid glycoside ointment group has significantly improved hair growth score and statistical significance (P < 0.01). The vaccarin ointment increased hair growth score compared to the positive drug minoxidil, but had no statistical significance (P ═ 0.259). The vaccaria flavonoid glycoside oral group was similar to the minoxidil score with no significant difference (P0.804), but it was only slightly higher (P0.3674) than the model group. The cowherb seed flavonoid glycoside can apparently inhibit alopecia caused by chemotherapy drugs, and the ointment has better effect than minoxidil and oral preparations.

Then, the hair at 10 fixed positions on the back of the mouse is carefully nipped by using tweezers, about 10 hairs are picked by using tweezers each time, the tweezers are placed on clean white paper, the vernier caliper measures the length, the longest value is selected as a recorded value, and the whole measuring process is repeated for 2 times.

As a result, as shown in fig. 4, the vaccaria flavonoid glycoside ointment group significantly increased hair length compared to the model group, with statistical significance (. about.p < 0.01). The cowherb flavonoid glycoside ointment group was able to increase hair length compared to the positive drug minoxidil, but had no statistical significance (P. 0.0907). The vaccaria flavonoid glycoside oral group also significantly increased hair length (═ P ═ 0.0036), slightly weaker than minoxidil, but with no significant difference. It is shown that the cowherb seed flavonoid glycoside can inhibit the hair growth retardation caused by the chemotherapeutic drugs and correspondingly slightly increase some hair length.

As a result, as shown in fig. 5, the vaccaria flavonoid glycoside ointment group significantly increased the remaining hair weight compared to the model group, with statistical significance (. about.p < 0.001). Compared with the positive drug minoxidil, the cowherb seed flavonoid glycoside ointment group can increase the remaining hair weight, but has no statistical significance (P ═ 0.168). The vaccaria flavonoid glycoside oral group also increased hair weight (P <0.01) compared to the model group. The cowherb seed flavonoid glycoside can inhibit alopecia caused by chemotherapy drugs.

Example 3: experimental study on evaluation of curative effect of cowherb seed flavonoid glycoside on C57BL/6 mouse androgen alopecia model

1. Laboratory animal

The experimental animals are C57BL/J6, 8 weeks old and 18-20g in weight, and are purchased from the comparative medical center of Yangzhou university. The C57BL/6 mouse is bred under normal conditions, the temperature is 25 +/-2 ℃, the humidity is 50% -70%, the illumination and the darkness are carried out alternately for 12h respectively, the mouse can eat and drink water freely, the corn cob padding is replaced every two days, and the experiment is started after the mouse adapts to the breeding environment for 7 days.

2. Drug configuration

The 4% chloral hydrate was prepared as above.

The ointment of the cowherb seed flavonoid glycoside is prepared as above.

Testosterone propionate injection (1 mL: 25mg, Guangzhou Baiyunshanming pharmaceutical Co., Ltd.) was diluted to 1mg/mL with edible oil before use. And (3) diluting the testosterone propionate diluent, and oscillating the diluted testosterone propionate diluent for 2 minutes by using a vortex mixer to fully and uniformly mix the diluted testosterone propionate diluent and the testosterone propionate diluent for later use.

Modeling of AGA mice

Injection of testosterone propionate was performed subcutaneously in the back of the mice parallel to the spinal area at a dose of 5mg/kg d. After dilution to 1mg/mL, 100mL was injected subcutaneously per mouse once a day for 28 days.

C57BL/J6 mouse androgen alopecia animal model modeling success judgment standard: 1 the back hair is exfoliated and partially fused into large pieces. 2 significant seborrhea was visible on the back. Pathological manifestations of the tissue, a smaller proportion of terminal hair/vellus hair, atrophy, degeneration and disappearance of hair follicles. The above 3 pieces are met at the same time, and the model can be considered to be successful.

Treatment experiment of AGA depilated mice

24 depilated mice were randomized into 4 groups of 6 mice: a control group, a cowherb seed flavonoid glycoside ointment group, a cowherb seed flavonoid glycoside oral group and a minoxidil group. Control group was given a blank cream without drug; the administration concentration of the cowherb seed flavonoid glycoside group is 2.5%, and about 0.2g of cotton swab dipping paste is used for applying to a depilation area in each administration; the concentration of commercially available minoxidil tincture was 5%, and the solution was diluted to 2.5% by adding an equal volume of anhydrous ethanol, and the dose was 200. mu.l per one. The treatment was performed simultaneously with the AGA molding, and the same administration was performed for 28 days.

Mice were scored by photographing after they were anesthetized with isoflurane every week from the start of molding.

5. Experimental study for evaluating curative effect of cowherb seed flavonoid glycoside on AGA alopecia of depilatory mouse

Photographs of hair growth were scored weekly, as described above. Grading standard: the remaining hair area is a proportion of the total area of the epilation area.

The results are shown in fig. 6, and compared with the blank cream control group, the cowherb seed flavonoid glycoside ointment group has significantly improved hair growth curve score. The vaccaria flavonol glycoside ointment also increased hair growth scores compared to the positive drug minoxidil. Similarly, the cowherb seed flavonoid glycoside oral group also performed better than minoxidil, but there was no significant difference. The cowherb seed flavonoid glycoside can apparently inhibit androgen alopecia.

Carefully forceps the hair at 10 fixed positions on the back of the mouse by using forceps, taking about 10 hairs by using forceps each time, placing the hairs on clean white paper, measuring the length by using a vernier caliper, taking the longest value as a recorded value, and repeating the whole measuring process for 2 times.

The results are shown in fig. 7, where the vaccaria flavonoid glycoside ointment group significantly increased hair length compared to the blank cream control group, with statistical significance (. about.p < 0.05). Compared to the positive drug minoxidil, the cowherb seed flavonoid glycoside ointment is long, but not statistically significant (P. 0.3597). The cowherb seed flavonoid glycoside oral group can also obviously increase the hair length of mice. In conclusion, the cowherb seed flavonoid glycoside can inhibit androgenetic alopecia.

The results are shown in fig. 8, the cowherb flavonoid glycoside ointment group significantly increased the remaining hair weight compared to the blank cream control group, with statistical significance (. about.p < 0.01). The cowherb seed flavonoid glycoside ointment group was able to increase the remaining hair weight compared to the positive drug minoxidil, but had no statistical significance (P. 0.1243). The cowherb seed flavonoid glycoside oral group also has certain effect similar to that of minoxidil. The cowherb seed flavonoid glycoside can inhibit androgen alopecia and maintain maximum hair weight.

The embodiments of the present invention have been described above. However, the present invention is not limited to the above embodiment. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. Use of vaccaria flavonoid glycosides and physiologically acceptable salts or solvates thereof for the preparation of a composition for dermal application.

2. The use according to claim 1, wherein said dermally applied composition is a composition having a synergistic effect of one or more of antiandrogen, hair growth stimulating, anti-sebum effects; preferably, the skin application composition is an antiandrogen composition; or the skin application composition is a composition for stimulating hair growth; or the skin application composition is an anti-sebum composition.

3. Use according to claim 1 or 2, wherein the skin comprises tissue covering the surface of the human or animal body, further comprising tissue covering the surface of the face or body, as well as the scalp and hair; the hair encompasses body hair and head hair; preferably, the skin is the scalp; preferably the hair is hair.

4. The use according to any one of claims 1 to 3, wherein said stimulating hair growth comprises stimulating the production of new hair, and promoting the growth of existing hair in a healthy state.

5. The use according to any one of claims 1 to 4, wherein said dermatological composition is capable of inhibiting androgens, stimulating the proliferation of hair follicle papilla cells and hair growth of hair follicles, while controlling sebum hypersecretion; the anti-sebum composition can be applied to condition or improve skin problems caused by excessive sebaceous gland secretion; the skin application composition is used for preventing and/or treating alopecia after chemotherapy of a patient; the dermally administrable composition is used for preventing and/or treating androgenic alopecia; the skin application composition is used for preventing and/or treating alopecia caused by autoimmune disease alopecia areata.

6. Use of the dermally administrable composition according to any one of claims 1 to 5 in pharmaceutical preparations, cosmetics, care products, cosmetics.

7. The use according to claim 6, in the prevention, care and treatment of oligotrichia, alopecia and to promote hair health and growth, to suppress sebum secretion; preferably, the pharmaceutical preparation can be topically applied through skin, and the dosage form of the preparation is selected from cream, patch, ointment, cream preparation, gel and spray, and can also be orally taken, and the oral preparation comprises tablet, granule, capsule, oral liquid preparation, pill, suspension, dripping pill and the like.

8. The use according to claim 7, the pharmaceutical formulation further comprising a physiologically acceptable carrier.

9. The use according to claim 6, wherein the cowherb seed flavonoid glycoside and the physiologically acceptable salt or solvate thereof accounts for 3-7 wt% of the total weight of the preparation.

10. Use according to claim 6, wherein the cosmetic, care or cosmetical product is selected from shampoos, conditioners, hair treatments, hair treatment rinses, hair oils, hair lotions, scalp tonics, hair essences, scalp creams, hair gels, hair sprays, hair films, eyebrow rinses.