CN112057410B - Hand disinfectant and preparation method thereof - Google Patents
Hand disinfectant and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a hand disinfectant and a preparation method thereof, the hand disinfectant is prepared from a surfactant, polyethylene glycol-2000, a silkworm pupa extract, succinic acid, sodium alginate, betaine, antibacterial peptide and deionized water, and the sterilization rate of the hand disinfectant on escherichia coli, staphylococcus albus, staphylococcus aureus and pseudomonas aeruginosa can reach 99.99%; the effective sterilization components of the hand disinfectant are the silkworm chrysalis extract and the antimicrobial peptide, the surfactant disclosed by the invention has a certain killing power on bacteria, the greater effect is embodied in that the killing capability of the silkworm chrysalis extract on the bacteria can be assisted, the effective components of the silkworm chrysalis extract can be well protected from inactivation after long-term existence, and the silkworm chrysalis extract, the antimicrobial peptide and the surfactant have a synergistic effect; the hand disinfectant has scientific proportioning and good sterilizing effect, and can not generate drug resistance.
Description
Technical Field
The invention relates to the technical field of disinfectants, and particularly relates to a hand disinfectant and a preparation method thereof.
Background
The disinfectant is used for killing pathogenic microorganisms on the transmission medium, so that the harmless requirement is met, the pathogenic microorganisms are killed out of the human body, the transmission path of infectious diseases is cut off, and the purpose of controlling the infectious diseases is achieved; most of the hand disinfectants on the market at present are simple to manufacture, low in price and single in function, and can only be used simply.
The silkworm pupa contains various natural antibacterial substances and tussah natural antibacterial substances, only one antibacterial peptide can be separated by using the recombinant antibacterial peptide gene production, and various antibacterial substances in the silkworm pupa cannot be effectively utilized.
The antibacterial peptide is a small molecular active peptide widely existing in organisms in the nature, and has the advantages of small molecular weight, broad-spectrum antibacterial property, unique antibacterial mechanism and the like compared with the traditional antibiotics. The melittin has killing activity to bacteria, fungi, viruses, mycoplasma, chlamydia, spirochetes and some active cells, and also has biological functions of resisting infection, regulating immunity, signal transduction and the like, is easy to dissolve in water, plays a main pharmacological role in bee venom, and is one of substances with the strongest anti-inflammation property known to human beings at present. It has hormone-like action, but has no adverse side effects. In addition, it also has antibacterial, antiradiation, and antivirus effects; although antibacterial peptides have a strong antibacterial effect, the antibacterial activity of the antibacterial peptides cannot be maintained all the time, and the antibacterial activity of the antibacterial peptides is influenced by many factors, such as: environmental factors, temperature, formulation, etc.
Disclosure of Invention
The invention provides a hand disinfectant and a preparation method thereof, the hand disinfectant has good bactericidal effect, and the activity of a silkworm chrysalis extract is maintained by cooperatively sterilizing the silkworm chrysalis extract and antibacterial peptide and assisting with a surfactant with certain bactericidal capacity.
The invention adopts the following technical scheme for solving the technical problems:
a hand disinfectant is prepared from the following raw materials in parts by weight: 5-8 parts of surfactant, 2-4 parts of polyethylene glycol-2000, 0.2-0.4 part of silkworm chrysalis extract, 0.5-1.5 parts of succinic acid, 0.5-1 part of sodium alginate, 0.3-0.6 part of betaine, 0.01-0.03 part of antibacterial peptide and 80-90 parts of deionized water.
As a most preferable scheme, the hand disinfectant is prepared from the following raw materials in parts by weight: 6 parts of surfactant, 3 parts of polyethylene glycol-2000, 0.3 part of silkworm chrysalis extract, 0.8 part of succinic acid, 0.6 part of sodium alginate, 0.4 part of betaine, 0.02 part of antibacterial peptide and 85 parts of deionized water.
Preferably, the antibacterial peptide is one or two of defensin and melittin.
As a most preferred embodiment, the antimicrobial peptide is melittin.
The applicant of the invention finds that the melittin and the silkworm chrysalis extract have a synergistic effect in experiments, the melittin has a sterilization effect and can improve the sterilization effect of the silkworm chrysalis extract, and the melittin and the silkworm chrysalis extract have a synergistic effect.
As a preferable scheme, the surfactant is one or more of sodium dodecyl benzene sulfonate, sodium dodecyl sulfonate, sodium alpha-alkenyl sulfonate, alkyl glycoside, tween 60 and tween 80.
As a preferable scheme, the surfactant is alkyl glycoside, sodium alpha-alkenyl sulfonate and tween 60 in a weight ratio of 1: 1-1.5: 2-3 mixing.
As a most preferred scheme, the surfactant is alkyl glycoside, sodium alpha-alkenyl sulfonate, tween 60 in a weight ratio of 1: 1.2: and 2.5, mixing.
As a preferred scheme, the preparation method of the silkworm chrysalis extract comprises the following steps:
(1) treating the silkworm chrysalis in an ultrasonic generator with the frequency of 40-50 KHz and the power of 350-450W for 20-40 min, and after ultrasonic treatment, putting the silkworm chrysalis into a thermostat with the temperature of 40-60 ℃ for 4-6 days;
(2) soaking the silkworm chrysalis in an acetone aqueous solution with the concentration of 8-12 wt% for 10-14 h, freeze-drying the silkworm chrysalis, and crushing the silkworm chrysalis to 150-250 meshes to obtain crude silkworm chrysalis powder;
(3) adding the crude silkworm chrysalis powder into absolute ethyl alcohol, placing the mixture in a water bath at the temperature of 50-70 ℃ for 20-40 min, centrifuging and drying to obtain the silkworm chrysalis powder;
(4) according to the weight ratio of 1: 3-5, adding the silkworm chrysalis powder into deionized water to obtain a mixed solution, wherein the weight ratio of the silkworm chrysalis powder to the deionized water is 1: 1-1.2 adding petroleum ether into the mixed solution for extraction, collecting an extract phase, concentrating under reduced pressure to 1.0-1.5 g/ml, and drying to obtain the silkworm chrysalis extract.
As a preferable scheme, the weight ratio of the crude silkworm chrysalis powder to the absolute ethyl alcohol in the step (3) is 1: 4 to 6.
As a most preferable scheme, the weight ratio of the crude silkworm chrysalis meal to the absolute ethyl alcohol in the step (3) is 1: 5.
the invention also provides a preparation method of the hand disinfectant, which comprises the following steps:
(1) adding a surfactant into deionized water, heating to 70-90 ℃, and uniformly stirring;
(2) cooling to 50-65 ℃, adding succinic acid, sodium alginate and betaine, and uniformly stirring;
(3) cooling to 35-45 ℃, adding polyethylene glycol-2000, the silkworm chrysalis extract and the antibacterial peptide, and uniformly stirring to obtain the hand sanitizer.
Confirming the optimal ratio of the melittin to the silkworm pupa extract, firstly weighing 0.3 part of the silkworm pupa extract, weighing 6 parts of surfactant, 3 parts of polyethylene glycol-2000, 0.3 part of the silkworm pupa extract, 0.8 part of succinic acid, 0.6 part of sodium alginate, 0.4 part of betaine and 85 parts of deionized water according to the weight part ratio, wherein the surfactant is sodium dodecyl benzene sulfonate, alkyl glycoside and tween 80 which are mixed according to the weight ratio of 1:2: 3; on the basis of the formula, 0 part of melittin, 0.005 part of melittin, 0.010 part of melittin, 0.015 part of melittin, 0.020 part of melittin, 0.025 part of melittin, 0.030 part of melittin and 0.35 part of melittin are respectively added to prepare disinfectants, and the disinfectant is tested for quantitative sterilization tests of suspensions according to the specification of 2008 edition 'sterilization technical Specification', wherein the prepared disinfectants have the sterilization effects on staphylococcus aureus and staphylococcus albus, and the specific detection method and the results are as follows.
Experimental strains: staphylococcus albus (8032), Staphylococcus aureus (ATCC 6538).
The operation method comprises the following steps: respectively and uniformly coating the 12-hour liquid cultures of the four bacteria on the surface of a common plate by using sterile cotton swabs, slightly drying, clamping and taking drug sensitive paper sheets with quantitative sample liquid medicine by using sterile tweezers, flatly paving the drug sensitive paper sheets on the surface of the plate inoculated with the bacteria, placing 3 pieces of drug sensitive paper sheets on one plate, culturing at 37 ℃, observing and recording the result after 2 hours. The test results are shown in FIG. 1; wherein, the sterilization rate = (average bacteria number of sample before sterilization-average bacteria number of sample after sterilization)/average bacteria number of sample before sterilization.
The results show that the peak value is not reached even though the good effect is achieved when the weight parts of melittin are 0.010 part and 0.015 part, and the peak value is achieved when the weight part of melittin is 0.020 part, so the optimal mixture ratio is that the weight part of melittin is 0.020 part.
The invention has the beneficial effects that: (1) the hand disinfectant is prepared from a surfactant, polyethylene glycol-2000, a silkworm chrysalis extract, succinic acid, sodium alginate, betaine, antibacterial peptide and deionized water, and has a sterilization rate of 99.99% for escherichia coli, staphylococcus albus, staphylococcus aureus and pseudomonas aeruginosa; (2) the effective bactericidal components of the hand disinfectant are the silkworm chrysalis extract and the antimicrobial peptide, the surfactant disclosed by the invention has a certain killing power on bacteria, and the greater effect is embodied in that the killing capability of the silkworm chrysalis extract on the bacteria can be assisted, the effective components of the silkworm chrysalis extract can be well protected from inactivation after long-term existence, the active protective agent can be played in a formula, and the silkworm chrysalis extract, the antimicrobial peptide and the surfactant have a synergistic effect; (3) the hand disinfectant has scientific proportioning and good sterilizing effect, and can not generate drug resistance.
Drawings
FIG. 1 shows the sterilization rates of different melittin formulations, where the left bar of the histogram shows the sterilization rate of Staphylococcus albus and the right bar shows the sterilization rate of Staphylococcus aureus.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are a part of the embodiments of the present invention, but not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without inventive step based on the embodiments of the present invention, are within the scope of protection of the present invention.
The defensins of the invention are purchased from Guangzhou and Shikang biotechnologies Inc.; the melittin was purchased from Zhejiang surge volume peptide biology, Inc., as promised by Guangzhou Probiotics technology, Inc.
Example 1
The hand disinfectant is prepared from the following raw materials in parts by weight: 6 parts of surfactant, 3 parts of polyethylene glycol-2000, 0.3 part of silkworm chrysalis extract, 0.8 part of succinic acid, 0.6 part of sodium alginate, 0.4 part of betaine, 0.02 part of antibacterial peptide and 85 parts of deionized water.
The antibacterial peptide is melittin.
The surfactant is alkyl glycoside, alpha-sodium alkenyl sulfonate and tween 60 in a weight ratio of 1: 1.2: 2.5 mixing.
The preparation method of the silkworm chrysalis extract comprises the following steps:
(1) treating silkworm pupa in 400W ultrasonic generator with frequency of 45KHz for 30min, and placing the silkworm pupa in 50 deg.C thermostat for 5d after ultrasonic treatment;
(2) soaking the silkworm chrysalis in a 10wt% acetone aqueous solution for 12 hours, freeze-drying the silkworm chrysalis, and crushing the silkworm chrysalis to 200 meshes to obtain crude silkworm chrysalis powder;
(3) adding the crude silkworm chrysalis powder into absolute ethyl alcohol, wherein the weight ratio of the crude silkworm chrysalis powder to the absolute ethyl alcohol is 1: 5, placing the silkworm chrysalis in a water bath at 60 ℃ for 30min, centrifuging and drying to obtain silkworm chrysalis powder;
(4) according to the weight ratio of 1: 4, adding the silkworm chrysalis powder into deionized water to obtain a mixed solution, wherein the weight ratio of the silkworm chrysalis powder to the deionized water is 1: 1.1 adding petroleum ether into the mixed solution for extraction, collecting an extract phase, concentrating under reduced pressure to 1.2g/ml, and drying to obtain the silkworm chrysalis extract.
The preparation method of the hand sanitizer comprises the following steps:
(1) adding a surfactant into deionized water, heating to 80 ℃, and uniformly stirring;
(2) cooling to 60 deg.C, adding succinic acid, sodium alginate and betaine, and stirring;
(3) cooling to 40 deg.C, adding polyethylene glycol-2000, pupa Bombycis extract, and antibacterial peptide, and stirring to obtain the hand disinfectant.
Example 2
A hand disinfectant is prepared from the following raw materials in parts by weight: 5 parts of surfactant, 2 parts of polyethylene glycol-2000, 0.2 part of silkworm chrysalis extract, 0.5 part of succinic acid, 0.5 part of sodium alginate, 0.3 part of betaine, 0.01 part of antibacterial peptide and 80 parts of deionized water.
The antibacterial peptide is composed of defensin and melittin according to the weight ratio of 1: 1.
The surfactant is prepared by mixing sodium dodecyl benzene sulfonate, alkyl glycoside and tween 80 according to the weight ratio of 1:2: 3.
The preparation method of the silkworm chrysalis extract comprises the following steps:
(1) treating silkworm pupa in an ultrasonic generator with frequency of 40KHz and power of 350W for 20min, and after ultrasonic treatment, putting the silkworm pupa into a thermostat with temperature of 40 ℃ for 4 days;
(2) soaking the silkworm chrysalis in an acetone aqueous solution with the concentration of 8wt% for 10 hours, freeze-drying the silkworm chrysalis, and crushing the silkworm chrysalis to 150 meshes to obtain crude silkworm chrysalis powder;
(3) adding the crude silkworm chrysalis powder into absolute ethyl alcohol, wherein the weight ratio of the crude silkworm chrysalis powder to the absolute ethyl alcohol is 1: 4, placing in a water bath at 50 ℃ for 20min, centrifuging and drying to obtain the silkworm chrysalis powder;
(4) according to the weight ratio of 1: adding the silkworm chrysalis powder into deionized water to obtain a mixed solution, wherein the weight ratio of the silkworm chrysalis powder to the deionized water is 1:1 adding petroleum ether into the mixed solution for extraction, collecting an extract phase, concentrating under reduced pressure to 1.0g/ml, and drying to obtain the silkworm chrysalis extract.
The preparation method of the hand sanitizer comprises the following steps:
(1) adding a surfactant into deionized water, heating to 80 ℃, and uniformly stirring;
(2) cooling to 60 deg.C, adding succinic acid, sodium alginate and betaine, and stirring;
(3) cooling to 40 deg.C, adding polyethylene glycol-2000, pupa Bombycis extract, and antibacterial peptide, and stirring to obtain the hand disinfectant.
Example 3
A hand disinfectant is prepared from the following raw materials in parts by weight: 7 parts of surfactant, 4 parts of polyethylene glycol-2000, 0.4 part of silkworm chrysalis extract, 1.5 parts of succinic acid, 1 part of sodium alginate, 0.5 part of betaine, 0.03 part of antibacterial peptide and 90 parts of deionized water.
The antibacterial peptide is defensin.
The surfactant is tween 60.
The preparation method of the silkworm chrysalis extract comprises the following steps:
(1) treating silkworm pupa in an ultrasonic generator with frequency of 50KHz and 450W for 40min, and after ultrasonic treatment, putting the silkworm pupa into a thermostat with the temperature of 60 ℃ for 6 d;
(2) soaking the silkworm chrysalis in a 12wt% acetone aqueous solution for 14h, freeze-drying the silkworm chrysalis, and crushing the silkworm chrysalis to 250 meshes to obtain crude silkworm chrysalis powder;
(3) adding the crude silkworm chrysalis powder into absolute ethyl alcohol, wherein the weight ratio of the crude silkworm chrysalis powder to the absolute ethyl alcohol is 1: 4-6; placing in 70 deg.C water bath for 40min, centrifuging, and drying to obtain silkworm pupa powder;
(4) according to the weight ratio of 1: 5, adding the silkworm chrysalis powder into deionized water to obtain a mixed solution, wherein the weight ratio of the silkworm chrysalis powder to the deionized water is 1: 1.2 adding petroleum ether into the mixed solution for extraction, collecting an extract phase, concentrating under reduced pressure to 1.5g/ml, and drying to obtain the silkworm chrysalis extract.
The preparation method of the hand sanitizer comprises the following steps:
(1) adding a surfactant into deionized water, heating to 80 ℃, and uniformly stirring;
(2) cooling to 60 deg.C, adding succinic acid, sodium alginate and betaine, and stirring;
(3) cooling to 40 deg.C, adding polyethylene glycol-2000, pupa Bombycis extract, and antibacterial peptide, and stirring to obtain the hand disinfectant.
Comparative example 1
Comparative example 1 is different from example 1 in that comparative example 1 does not contain the silkworm chrysalis extract, and the others are the same.
Comparative example 2
Comparative example 2 is different from example 1 in that comparative example 2 does not contain an antimicrobial peptide, and the others are the same.
Comparative example 3
Comparative example 3 differs from example 1 in that comparative example 3 does not contain a surfactant, and is otherwise the same.
Comparative example 4
Comparative example 4 is different from example 1 in that the silkworm chrysalis extract of comparative example 4 is different from example 1, and the rest is the same.
The preparation method of the silkworm chrysalis extract comprises the following steps:
(1) soaking the silkworm chrysalis in a 12wt% acetone aqueous solution for 14h, freeze-drying the silkworm chrysalis, and crushing the silkworm chrysalis to 250 meshes to obtain crude silkworm chrysalis powder;
(2) adding the crude silkworm chrysalis powder into absolute ethyl alcohol, wherein the weight ratio of the crude silkworm chrysalis powder to the absolute ethyl alcohol is 1: 4-6; placing in 70 deg.C water bath for 40min, centrifuging, and drying to obtain domestic pupa Bombycis powder;
(3) according to the weight ratio of 1: 5, adding the silkworm chrysalis powder into deionized water to obtain a mixed solution, wherein the weight ratio of the silkworm chrysalis powder to the deionized water is 1: 1.2 adding petroleum ether into the mixed solution for extraction, collecting an extract phase, concentrating under reduced pressure to 1.5g/ml, and drying to obtain the silkworm chrysalis extract.
Comparative example 5
Comparative example 5 is different from example 1 in that the silkworm chrysalis extract of comparative example 5 is different from example 1, and the rest is the same.
(1) Treating silkworm pupa in 400W ultrasonic generator with frequency of 45KHz for 30min, and placing the silkworm pupa in 50 deg.C thermostat for 5d after ultrasonic treatment;
(2) soaking the silkworm chrysalis in a 10wt% acetone aqueous solution for 12 hours, freeze-drying the silkworm chrysalis, and crushing the silkworm chrysalis to 200 meshes to obtain crude silkworm chrysalis powder;
(3) according to the weight ratio of 1: 4, adding the crude silkworm chrysalis powder into deionized water to obtain a mixed solution, wherein the weight ratio of the raw material to the mixed solution is 1: 1.1 adding petroleum ether into the mixed solution for extraction, collecting an extract phase, concentrating under reduced pressure to 1.2g/ml, and drying to obtain the silkworm chrysalis extract.
To further demonstrate the effect of the present invention, the following test methods were provided:
during testing, the hand disinfectant and the deionized water are mixed according to the weight ratio of 1: 9 mixing and filling into a spray bottle.
1. Irritation test
Taking 40 mice, randomly dividing into 8 groups, using 5 mice in each group, dipping the disinfectants in the examples 1-3 and the comparative examples 1-5 by a brush respectively, smearing on the back until the mice are thoroughly wetted by hairs, and observing skin reaction after 2 hours, wherein the smearing area is 3.5 multiplied by 3.5 cm; if the white mouse is smeared, the white mouse will show congestion, edema and red swelling, which is considered as irritant by the disinfectant.
The results show that the white mice of the examples 1-3 and the comparative examples 1-5 have no hyperemia, edema and red swelling, and the disinfectants of the examples 1-3 and the comparative examples 1-5 have no irritation.
2. Sterilization test
According to a suspension quantitative sterilization test specified in 2008 edition disinfection technical Specification, the sterilization effects of the disinfectants prepared in examples 1-3 and comparative examples 1-5 on escherichia coli, staphylococcus aureus, staphylococcus albus and pseudomonas aeruginosa are detected, and the specific detection method and results are as follows.
Experimental strains: escherichia coli (8099), Staphylococcus albus (8032), Staphylococcus aureus (ATCC 6538), and Pseudomonas aeruginosa (ATCC 15442).
The operation method comprises the following steps: respectively and uniformly coating the 12-hour liquid cultures of the four bacteria on the surface of a common plate by using sterile cotton swabs, slightly drying, clamping and taking drug sensitive paper sheets with quantitative sample liquid medicine by using sterile tweezers, flatly paving the drug sensitive paper sheets on the surface of the plate inoculated with the bacteria, placing 3 pieces of drug sensitive paper sheets on one plate, culturing at 37 ℃, observing and recording the result after 2 hours. The test results are shown in Table 1; wherein, the sterilization rate = (average bacteria number of sample before sterilization-average bacteria number of sample after sterilization)/average bacteria number of sample before sterilization.
TABLE 1 Sterilization rates
As can be seen from the table 1, the disinfectant disclosed by the invention has a good sterilization effect, and can effectively sterilize escherichia coli, staphylococcus albus, staphylococcus aureus and pseudomonas aeruginosa, and the comparative examples 1-3 show that the preparation parameters of the silkworm chrysalis extract and the proportion of the raw materials of the disinfectant can affect the sterilization effect of the disinfectant within a certain range; compared with the comparative examples 1 and 1, the silkworm chrysalis extract disclosed by the invention can obviously influence the killing of a disinfectant on escherichia coli, staphylococcus albus, staphylococcus aureus and pseudomonas aeruginosa; comparing example 1 with comparative example 2, it can be seen that the antibacterial peptide can significantly improve the killing of the disinfectant of the present invention on escherichia coli, staphylococcus albus, staphylococcus aureus and pseudomonas aeruginosa; comparing example 1 with comparative example 3, it can be seen that the surfactant of the present invention can significantly improve the killing of the disinfectant of the present invention on escherichia coli, staphylococcus albus, staphylococcus aureus, and pseudomonas aeruginosa; wherein the effect of the antimicrobial peptide is greater than that of the surfactant; therefore, the silkworm chrysalis extract, the antibacterial peptide and the surfactant have a synergistic effect on killing of escherichia coli, staphylococcus albus, staphylococcus aureus and pseudomonas aeruginosa; compared with the comparative examples 4 and 5, the preparation method of the silkworm chrysalis extract has a remarkable improvement effect on escherichia coli, staphylococcus albus, staphylococcus aureus and pseudomonas aeruginosa.
3. Stability test
The disinfectant described in examples 1-3 and comparative examples 1-5 was placed at 55 ℃ for 30 days to measure the bactericidal effect again, the test method was the method described above, and the test results are shown in table 2.
Table 2 stability test results
Comparing the data in table 2 with the data in table 1, it can be seen that the sterilizing rates of the disinfectants in examples 1-3, comparative examples 1-2, and comparative examples 4-5 to escherichia coli, staphylococcus albus, staphylococcus aureus, and pseudomonas aeruginosa hardly decrease after 30 days at 55 ℃, but the disinfectant in comparative example 3 decreases significantly, and it can be seen that the selection of the surfactant of the present invention can assist the sterilization of the silkworm chrysalis extract and the antimicrobial peptide, and the silkworm chrysalis extract can maintain the activity.
4. Drug resistance testing
The disinfection test site is a farmer room, bacteria samples in the air of each test site are collected respectively by a natural sedimentation method, namely 5 common agar plates are respectively taken, (the surface area of each plate is 58 mm) 2 ) Respectively placing the above materials at the periphery and center of the above place, opening the flat cover, exposing for 10min, covering the flat cover, placing in 37 deg.C incubator, culturing for 48 hr, calculating bacterial colony number, and determining bacterial count contained in air per cubic meter. Then, the sterilization was performed using the disinfectant described in example 1, the number of bacteria was measured once again in the above method after 2 hours, the sterilization rate was calculated, the living room of the breeder was sterilized by continuously using the same disinfectant for 30 days (the living room of the breeder was sterilized every three days from the first day test), and the sterilization rate was measured after 30 days in 2 hours after the sterilization, and the test results are shown in table 3.
TABLE 3 drug resistance test
As shown in Table 3, the disinfectant of the present invention can effectively disinfect the indoor, the disinfection rate reaches 99.89% on day 1, and the disinfection rate reaches 99.82% on day 31 when the disinfectant is used every three days, so that the disinfectant of the present invention does not generate drug resistance.
In light of the foregoing description of preferred embodiments according to the invention, it is clear that many changes and modifications can be made by the person skilled in the art without departing from the scope of the invention. The technical scope of the present invention is not limited to the contents of the specification, and must be determined according to the scope of the claims.
Claims (7)
1. The hand disinfectant is characterized by comprising the following raw materials in parts by weight: 5-8 parts of surfactant, 2-4 parts of polyethylene glycol-2000, 0.2-0.4 part of silkworm chrysalis extract, 0.5-1.5 parts of succinic acid, 0.5-1 part of sodium alginate, 0.3-0.6 part of betaine, 0.01-0.03 part of antibacterial peptide and 80-90 parts of deionized water;
the antibacterial peptide is melittin;
the preparation method of the silkworm chrysalis extract comprises the following steps:
(1) treating the silkworm chrysalis in an ultrasonic generator with the frequency of 40-50 KHz and the power of 350-450W for 20-40 min, and after ultrasonic treatment, putting the silkworm chrysalis into a thermostat with the temperature of 40-60 ℃ for 4-6 days;
(2) soaking the silkworm chrysalis in an acetone aqueous solution with the concentration of 8-12 wt% for 10-14 h, freeze-drying the silkworm chrysalis, and crushing the silkworm chrysalis to 150-250 meshes to obtain crude silkworm chrysalis powder;
(3) adding the crude silkworm chrysalis powder into absolute ethyl alcohol, placing the mixture in a water bath at the temperature of 50-70 ℃ for 20-40 min, centrifuging and drying to obtain the silkworm chrysalis powder;
(4) according to the weight ratio of 1: 3-5, adding the silkworm chrysalis powder into deionized water to obtain a mixed solution, wherein the weight ratio of the silkworm chrysalis powder to the deionized water is 1: 1-1.2, adding petroleum ether into the mixed solution for extraction, collecting an extract phase, concentrating under reduced pressure to 1.0-1.5 g/ml, and drying to obtain the silkworm chrysalis extract.
2. The hand sanitizer according to claim 1, wherein the hand sanitizer is prepared from the following raw materials in parts by weight: 6 parts of surfactant, 3 parts of polyethylene glycol-2000, 0.3 part of silkworm chrysalis extract, 0.8 part of succinic acid, 0.6 part of sodium alginate, 0.4 part of betaine, 0.02 part of antibacterial peptide and 85 parts of deionized water.
3. The hand sanitizer according to claim 1, wherein the surfactant is one or more of sodium dodecylbenzene sulfonate, sodium dodecylsulfate, sodium α -alkenyl sulfonate, alkyl glycoside, tween 60, tween 80.
4. The hand sanitizer according to claim 3, wherein the surfactant is an alkyl glycoside, sodium α -alkenyl sulfonate, tween 60 in a weight ratio of 1: 1-1.5: 2-3 mixing.
5. The hand sanitizer according to claim 1, wherein the weight ratio of the raw silkworm chrysalis meal to the absolute ethyl alcohol in the step (3) is 1: 4 to 6.
6. The hand sanitizer according to claim 1, wherein the weight ratio of the raw silkworm chrysalis meal to the absolute ethyl alcohol in the step (3) is 1: 5.
7. a process for the preparation of a hand sanitizer according to any of claims 1 to 6 comprising the steps of:
(1) adding a surfactant into deionized water, heating to 70-90 ℃, and uniformly stirring;
(2) cooling to 50-65 ℃, adding succinic acid, sodium alginate and betaine, and uniformly stirring;
(3) cooling to 35-45 ℃, adding polyethylene glycol-2000, the silkworm chrysalis extract and the antibacterial peptide, and uniformly stirring to obtain the hand sanitizer.
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