CN112056560A - 一种辅酶q10和褐藻寡糖氧化复合颗粒的制备方法 - Google Patents
一种辅酶q10和褐藻寡糖氧化复合颗粒的制备方法 Download PDFInfo
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Abstract
本发明公开了一种辅酶Q10和褐藻寡糖氧化复合颗粒的制备方法，由以下成分构成：褐藻寡糖、吐温80、辅酶Q10。本发明直接利用辅酶Q10分子与吐温80形成乳液，再与褐藻寡糖进行分子态结合，所制备的酶Q10和褐藻寡糖复合颗粒，具有稳定抗氧化的特点，不含有其它蛋白或氨基酸分子，可以避免可能产生的蛋白或氨基酸过敏反应或结抗反应，更有利于在食品、化妆品中选配，可直接应用于化妆品和食品方面。
Description
技术领域
本发明涉及食品、化妆品技术领域,更具体的说是涉及一种辅酶Q10和褐藻寡糖氧化复合颗粒的制备方法。
背景技术
辅酶Q10(Coenzyme Q10)是一类含对苯醌和多个异戊二烯组成的侧链化合物。为黄色至橙黄色结晶性粉末；无臭无味；遇光易分解。在三氯甲烷或丙酮中溶解，在乙醇中极微溶解，在水中不溶。结构如图1。辅酶Q10的独特机构使其存在氧化型和还原型，其还原型在细胞能被各种氧化剂或自由基氧化从而防止DNA、蛋白质和脂质被过度氧化达到抗氧化和去自由基功能(1)。辅酶Q10有助于预防充血性心力衰竭、脑血管闭塞以及抗癌药副作用(预防由阿霉素引起的心源性机能障碍)，同时有助于疲劳恢复、能量再生以及在活生物体中抗活性氧的氧化作用。此外，预计其作为皮肤外用而起作用，由此显示出抗衰老作用。辅酶Q10在自然保存条件下，由于氧化作用，会引起氧化、变质和抗氧化功能用降低等问题。因此，需要改善辅酶Q10遇光、热等易氧化、分解的问题。被包裹的辅酶Q10分子有效地防止辅酶Q10在自然状态下的氧化分解，最大程度上地维持其原有的性能，防止成分结构的破坏与损失。
褐藻寡糖Alginate oligosaccharides可分为β-D-聚甘露糖醛酸(M)和α-L-聚古罗糖醛酸(G)组成的线型低聚合物，有聚甘露糖醛酸(PM)、聚古罗糖醛酸(PG)和杂合褐藻寡糖(PMG)。聚合度为2-20，分子量为376-6500Da，能溶于水，稳定性强。具有抗氧化衰老、降血糖血脂、免疫和促进记忆等多种药理性质，褐藻胶寡糖具有多项生物学功能，在化妆品和食品方面有广泛的应用。
为了实现辅酶Q10分子抗氧化目的，很多采用颗粒包被技术。
欧洲专利No.494651B1和美国专利No.5298246公开了一种组合物，其通过将泛醌溶解在乳脂中而获得。
韩国专利No.10-0463167公开了一种将不溶性聚合物(例如聚甲基丙烯酸甲酯)和不溶于活生物体的辅酶QlO溶解在有机溶剂中，(例如，二氯甲烷)，通过溶剂萃取除去二氯甲烷并处理该乳剂。
这些技术存在制备复杂，需要高圧乳化，喷雾干燥等复杂设备，制备工序多、能耗高。
发明内容
有鉴于此，本发明提供了一种辅酶Q10和褐藻寡糖氧化复合颗粒的制备方法。
为实现上述目的，本发明提供如下技术方案，由以下成分构成：褐藻寡糖、吐温80、辅酶Q10。
优选的，在上述一种辅酶Q10和褐藻寡糖氧化复合颗粒的制备方法中，制备方法如下：
S1:称取褐藻寡糖10g于200ml去离子水中，加热至70℃，磁力搅拌，直至溶解，放冷至室温，得溶液A；
S2:用0.1ml NaOH溶液(3mol/L)调节溶液A的PH值至7.0，得到溶液B；
S3:取吐温801ml于100ml去离子水中，混匀，得到溶液C；
S4:称取辅酶Q1010g与上述C溶液中，磁力搅拌，混匀得到溶液D；
S5:上述溶液D，加入溶液B，磁力搅拌，混匀得到沉淀物E；
S6:沉淀物E经冷冻干燥，得辅酶Q10的褐藻寡糖包被混合物，经粉碎过120目筛，得辅酶Q10的褐藻寡糖包被颗粒。
优选的，在上述一种辅酶Q10和褐藻寡糖氧化复合颗粒的制备方法中，所述S1中褐藻寡糖溶解液为加热至70℃，磁力搅拌2-4h溶解。
优选的，在上述一种辅酶Q10和褐藻寡糖氧化复合颗粒的制备方法中，所述辅酶Q10乳混液为辅酶Q10与吐温80以10000r/min高速均质混匀溶液。
经由上述的技术方案可知，与现有技术相比，本发明公开提供了一种辅酶Q10和褐藻寡糖氧化复合颗粒的制备方法，本发明直接利用辅酶Q10分子与吐温80形成乳液，再与褐藻寡糖进行分子态结合，所制备的酶Q10和褐藻寡糖复合颗粒，具有稳定抗氧化的特点，不含有其它蛋白或氨基酸分子，可以避免可能产生的蛋白或氨基酸过敏反应或结抗反应，更有利于在食品、化妆品中选配，可直接应用于化妆品和食品方面。
附图说明
为了更清楚地说明本发明实施例或现有技术中的技术方案，下面将对实施例或现有技术描述中所需要使用的附图作简单地介绍，显而易见地，下面描述中的附图仅仅是本发明的实施例，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据提供的附图获得其他的附图。
图1附图为本发明的辅酶q10寡糖初始紫外可见光谱示意图。
图2附图为辅酶Q10褐藻寡糖1个月后紫外可见光谱示意图。
图3附图为本发明的辅酶Q10褐藻寡糖3个月后紫外可见光谱示意图。
具体实施方式
下面将结合本发明实施例中的附图，对本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都属于本发明保护的范围。
请参阅附图1-3，为本发明公开的一种辅酶Q10和褐藻寡糖氧化复合颗粒的制备方法中辅酶Q10褐藻寡糖紫外可见光谱示意图。
本发明，由以下成分构成：褐藻寡糖、吐温80、辅酶Q10。
为了进一步优化上述技术方案，制备方法如下：
S1:称取褐藻寡糖10g于200ml去离子水中，加热至70℃，磁力搅拌，直至溶解，放冷至室温，得溶液A；
S2:用0.1ml NaOH溶液(3mol/L)调节溶液A的PH值至7.0，得到溶液B；
S3:取吐温801ml于100ml去离子水中，混匀，得到溶液C；
S4:称取辅酶Q1010g与上述C溶液中，磁力搅拌，混匀得到溶液D；
S5:上述溶液D，加入溶液B，磁力搅拌，混匀得到沉淀物E；
S6:沉淀物E经冷冻干燥，得辅酶Q10的褐藻寡糖包被混合物，经粉碎过120目筛，得辅酶Q10的褐藻寡糖包被颗粒。
为了进一步优化上述技术方案，S1中褐藻寡糖溶解液为加热至70℃，磁力搅拌2-4h溶解。
为了进一步优化上述技术方案，辅酶Q10乳混液为辅酶Q10与吐温80以10000r/min高速均质混匀溶液。
为了进一步优化上述技术方案，辅酶Q10的褐藻寡糖抗氧化复合颗粒稳定性测定：
(1)依据美国FDA的要求标准,对于有效期为二年的产品,应在温度37-40℃、相对湿度75％以上的条件下保存三个月,经测试产品质量稳定方为合格。
对辅酶Q10的褐藻寡糖抗氧化复合颗粒进行了考察试验,采取温度为38～40℃,相对湿度75％～80％,在三个月保存期内。
分别于初始、30d和90d时，取1g上述复合颗粒溶于10ml去离子水中，用紫外可见光度法测定,用紫外可见分光光谱仪从190nm-700nm扫描，得紫外可见光谱。其结果如图1-3所示。光谱图表明辅酶Q10的褐藻寡糖抗氧化复合颗粒光谱特征保持稳定。
本说明书中各个实施例采用递进的方式描述，每个实施例重点说明的都是与其他实施例的不同之处，各个实施例之间相同相似部分互相参见即可。对于实施例公开的装置而言，由于其与实施例公开的方法相对应，所以描述的比较简单，相关之处参见方法部分说明即可。
对所公开的实施例的上述说明，使本领域专业技术人员能够实现或使用本发明。对这些实施例的多种修改对本领域的专业技术人员来说将是显而易见的，本文中所定义的一般原理可以在不脱离本发明的精神或范围的情况下，在其它实施例中实现。因此，本发明将不会被限制于本文所示的这些实施例，而是要符合与本文所公开的原理和新颖特点相一致的最宽的范围。
Claims (4)
1.一种辅酶Q10和褐藻寡糖氧化复合颗粒的制备方法，其特征在于，由以下成分构成：褐藻寡糖、吐温80、辅酶Q10。
2.根据权利要求1所述的一种辅酶Q10和褐藻寡糖氧化复合颗粒的制备方法，其特征在于，制备方法如下：
S1:称取褐藻寡糖10g于200ml去离子水中，加热至70℃，磁力搅拌，直至溶解，放冷至室温，得溶液A；
S2:用0.1ml NaOH溶液(3mol/L)调节溶液A的PH值至7.0，得到溶液B；
S3:取吐温80 1ml于100ml去离子水中，混匀，得到溶液C；
S4:称取辅酶Q1010g与上述C溶液中，磁力搅拌，混匀得到溶液D；
S5:上述溶液D，加入溶液B，磁力搅拌，混匀得到沉淀物E；
S6:沉淀物E经冷冻干燥，得辅酶Q10的褐藻寡糖包被混合物，经粉碎过120目筛，得辅酶Q10的褐藻寡糖包被颗粒。
3.根据权利要求1所述的一种辅酶Q10和褐藻寡糖氧化复合颗粒的制备方法，其特征在于，所述S1中褐藻寡糖溶解液为加热至70℃，磁力搅拌2-4h溶解。
4.根据权利要求1所述的一种辅酶Q10和褐藻寡糖氧化复合颗粒的制备方法，其特征在于，所述辅酶Q10乳混液为辅酶Q10与吐温80以10000r/min高速均质混匀溶液。
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