Detailed Description
The above summary of the present application is described in further detail below in conjunction with the detailed description, but it should not be understood that the scope of the above-described subject matter of the present application is limited to the following examples.
Introduction of partial raw materials in the application:
black pearl, commercially available black pearl powder (no boiling), was purchased from Jiansheng biotechnology Co., ltd.
Fresh cow milk, commercial pasteurized fresh cow milk was used.
The Jinjunmei black tea adopts the commercial Wuyi mountain Jinjunmei black tea.
The white rabbit toffee adopts commercially available crown garden white rabbit toffee.
Gelatin, food grade, CAS:9000-70-8, purity: 99%, purchased from Shandong Fengtai Biotechnology Co., ltd.
Defatted walnut powder, food grade, particle size: 80 mesh, purchased from Ningxia vanilla biotechnology Co.
Fucoidan, food grade, CAS:9072-19-9, purity: 99% of the product is purchased from Nantong fragrance adding bioengineering Co.
Pullulan, food grade, CAS:9057-02-7, purity: 98% purchased from Shanghai Beilang Biotech Co.
Pepsin, food grade, CAS:9001-75-6, 3800U/g, available from Siankono chemical Co., ltd.
The same raw materials are all the same in source.
Example 1
The mellow and thick flavored milk black tea comprises the following raw materials in parts by weight: 80 parts of Jinjunmei tea soup, 30 parts of fresh cow milk, 5 parts of white rabbit milk jelly, 5 parts of black pearl and 0.5 part of sucrose.
The preparation method of the mellow and thick flavored milk black tea comprises the following steps of:
step one: 1 part by weight of Jinjunmei black tea is made into a tea bag by gauze, then added into 50 parts by weight of purified water, extracted for 35s by a tea extraction machine at 2000rpm, and then Jinjunmei tea soup is obtained;
step two: adding 20 parts by weight of white rabbit toffee and 3.5 parts by weight of gelatin into 80 parts by weight of fresh cow milk, and stirring for 20min at 67 ℃ at a stirring speed of 50rpm; homogenizing at 5000rpm for 1min; pouring into a mold container for shaping, and refrigerating at 4deg.C for 4 hr to obtain white rabbit milk jelly;
step three: adding 30 parts by weight of fresh cow milk into 80 parts by weight of Jinjunmei tea soup, uniformly mixing, adding 0.5 part by weight of sucrose, uniformly mixing, and finally adding 5 parts by weight of black pearl and 5 parts by weight of white rabbit milk jelly, uniformly mixing, thus obtaining the pure and thick flavored cow milk black tea.
Example 2
The mellow and thick flavored milk black tea comprises the following raw materials in parts by weight: 160 parts of Jinjunmei tea soup, 100 parts of fresh cow milk, 25 parts of white rabbit milk jelly, 20 parts of black pearl and 5 parts of sucrose.
The preparation method of the mellow and thick flavored milk black tea comprises the following steps of:
step one: 1 part by weight of Jinjunmei black tea is made into a tea bag by gauze, then added into 50 parts by weight of purified water, extracted for 35s by a tea extraction machine at 2000rpm, and then Jinjunmei tea soup is obtained;
step two: adding 20 parts by weight of white rabbit toffee and 3.5 parts by weight of gelatin into 80 parts by weight of fresh cow milk, and stirring for 20min at 67 ℃ at a stirring speed of 50rpm; homogenizing at 5000rpm for 1min; pouring into a mold container for shaping, and refrigerating at 4deg.C for 4 hr to obtain white rabbit milk jelly;
step three: adding 100 parts by weight of fresh cow milk into 160 parts by weight of Jinjunmei tea soup, uniformly mixing, adding 5 parts by weight of sucrose, uniformly mixing, and finally adding 20 parts by weight of black pearl and 25 parts by weight of white rabbit milk jelly, and uniformly mixing to obtain the mellow and thick flavored cow milk black tea.
Example 3
The mellow and thick flavored milk black tea comprises the following raw materials in parts by weight: 100 parts of Jinjunmei tea soup, 50 parts of fresh cow milk, 12 parts of white rabbit milk jelly, 8 parts of black pearl and 2 parts of sucrose.
The preparation method of the mellow and thick flavored milk black tea comprises the following steps of:
step one: 1 part by weight of Jinjunmei black tea is made into a tea bag by gauze, then added into 50 parts by weight of purified water, extracted for 35s by a tea extraction machine at 2000rpm, and then Jinjunmei tea soup is obtained;
Step two: adding 20 parts by weight of white rabbit toffee and 3.5 parts by weight of gelatin into 80 parts by weight of fresh cow milk, and stirring for 20min at 67 ℃ at a stirring speed of 50rpm; homogenizing at 5000rpm for 1min; pouring into a mold container for shaping, and refrigerating at 4deg.C for 4 hr to obtain white rabbit milk jelly;
step three: adding 50 parts by weight of fresh cow milk into 100 parts by weight of Jinjunmei tea soup, uniformly mixing, adding 2 parts by weight of sucrose, uniformly mixing, and finally adding 8 parts by weight of black pearl and 12 parts by weight of white rabbit milk jelly, uniformly mixing, thus obtaining the pure and strong flavor cow milk black tea.
Test example 1
Sensory evaluation of the mellow and strong flavored milk black tea: 60 testers 16-50 years old were randomly selected, randomly divided into 4 groups of 15 individuals, and the flavored black milk tea of the examples were subjected to sensory evaluation. Each full score was 100 minutes and averaged.
Table 1 sensory evaluation (score) of mellow and strong flavored milk black tea
From the sensory evaluation results of the pure and thick flavored milk black tea in table 1, the flavored milk black tea disclosed by the invention takes Jinjunmei tea soup as a base, the Jinjunmei black tea is tight and beautiful in appearance, brown in color, fresh, alive, sweet and refreshing in taste, long in throat rhyme, and capable of improving human body circulation, sterilizing, resisting inflammation, refreshing and restoring consciousness, and is a precious product in tea and a top in black tea; the white rabbit milk jelly is matched with fresh cow milk, is fragrant, mellow, tasty, refreshing, delicious, perfectly neutral with the fragrance, toughness and delicious of black pearls, is light, smooth, warm in color and fragrant in lips and teeth, and is deeply favored by young people. The flavor and smooth taste of the tea are combined with the natural mellow and rich nutrition of the milk, so that the milk tea is complementary in nutrition and fragrant and smooth in taste.
Example 4
The mellow and thick flavored milk black tea comprises the following raw materials in parts by weight: 100 parts of Jinjunmei tea soup, 50 parts of fresh cow milk, 12 parts of white rabbit milk jelly, 8 parts of black pearl, 0.6 part of additive and 2 parts of sucrose.
The preparation method of the mellow and thick flavored milk black tea comprises the following steps of:
step one: 1 part by weight of Jinjunmei black tea is made into a tea bag by gauze, then added into 50 parts by weight of purified water, extracted for 35s by a tea extraction machine at 2000rpm, and then Jinjunmei tea soup is obtained;
step two: adding 20 parts by weight of white rabbit toffee and 3.5 parts by weight of gelatin into 80 parts by weight of fresh cow milk, and stirring for 20min at 67 ℃ at a stirring speed of 50rpm; homogenizing at 5000rpm for 1min; pouring into a mold container for shaping, and refrigerating at 4deg.C for 4 hr to obtain white rabbit milk jelly;
step three: adding 50 parts by weight of fresh cow milk into 100 parts by weight of Jinjunmei tea soup, uniformly mixing, adding 0.6 part by weight of additive and 2 parts by weight of sucrose, uniformly mixing, and finally adding 8 parts by weight of black pearl and 12 parts by weight of white rabbit jelly, and uniformly mixing to obtain the pure and thick flavored cow milk black tea.
The additive is functional walnut protein; the functional walnut protein is prepared by the following method:
(1) Adding defatted walnut powder into distilled water, uniformly mixing, wherein the mass ratio of the defatted walnut powder to the distilled water is 1:24, and regulating the pH value to 11.5 by using 2M NaOH; stirring at 56 ℃ for 12min at a stirring rate of 80rpm; ultrasonic treatment is carried out for 23min, and the ultrasonic frequency is 25kHz; stirring is continued for 70min at 56 ℃, and the stirring speed is 80rpm; centrifuging, collecting supernatant, adjusting pH to 4.3 with 2M HCl, and standing for 75min; centrifuging, filtering, and cleaning the filter residue with distilled water until the pH value of the cleaning liquid is 7.0; freeze drying to obtain walnut protein;
(2) Adding walnut protein into distilled water, and stirring for 100min at a stirring speed of 80rpm, wherein the mass ratio of the walnut protein to the distilled water is 5:100; homogenizing at 5000rpm for 2min, and standing for 50min; performing microwave treatment for 3min, wherein the microwave power is 800W; the pH was adjusted to 2.0 with 2M HCl; adding pepsin, wherein the mass ratio of the pepsin to the walnut protein is 0.3:100, and stirring for 65min at 37 ℃ with the stirring speed of 80rpm; heating at 95deg.C for 7min, and inactivating enzyme; adjusting the pH value of the solution to 7.0 by using 2M NaOH; centrifuging, collecting supernatant, and lyophilizing to obtain pretreated walnut protein;
(3) Adding a modifier into distilled water, and stirring for 7min at a stirring speed of 80rpm, wherein the mass ratio of the modifier to distilled water is 1:102; adding pretreated walnut protein, and continuously stirring for 113min, wherein the mass ratio of the pretreated walnut protein to the modifier is 1:2.5; freeze drying, and heating in a dryer for 135min at 130deg.C. The modifier is pullulan.
Comparative example 1
The mellow and thick flavored milk black tea comprises the following raw materials in parts by weight: 100 parts of Jinjunmei tea soup, 50 parts of fresh cow milk, 12 parts of white rabbit milk jelly, 8 parts of black pearl, 0.6 part of additive and 2 parts of sucrose.
The preparation method of the mellow and thick flavored milk black tea comprises the following steps of:
step one: 1 part by weight of Jinjunmei black tea is made into a tea bag by gauze, then added into 50 parts by weight of purified water, extracted for 35s by a tea extraction machine at 2000rpm, and then Jinjunmei tea soup is obtained;
step two: adding 20 parts by weight of white rabbit toffee and 3.5 parts by weight of gelatin into 80 parts by weight of fresh cow milk, and stirring for 20min at 67 ℃ at a stirring speed of 50rpm; homogenizing at 5000rpm for 1min; pouring into a mold container for shaping, and refrigerating at 4deg.C for 4 hr to obtain white rabbit milk jelly;
step three: adding 50 parts by weight of fresh cow milk into 100 parts by weight of Jinjunmei tea soup, uniformly mixing, adding 0.6 part by weight of additive and 2 parts by weight of sucrose, uniformly mixing, and finally adding 8 parts by weight of black pearl and 12 parts by weight of white rabbit jelly, and uniformly mixing to obtain the pure and thick flavored cow milk black tea.
The additive is functional walnut protein; the functional walnut protein is prepared by the following method:
(1) Adding defatted walnut powder into distilled water, uniformly mixing, wherein the mass ratio of the defatted walnut powder to the distilled water is 1:24, and regulating the pH value to 11.5 by using 2M NaOH; stirring at 56 ℃ for 105min at a stirring rate of 80rpm; centrifuging, collecting supernatant, adjusting pH to 4.3 with 2M HCl, and standing for 75min; centrifuging, filtering, and cleaning the filter residue with distilled water until the pH value of the cleaning liquid is 7.0; freeze drying to obtain walnut protein;
(2) Adding walnut protein into distilled water, and stirring for 100min at a stirring speed of 80rpm, wherein the mass ratio of the walnut protein to the distilled water is 5:100; homogenizing at 5000rpm for 2min, and standing for 50min; performing microwave treatment for 3min, wherein the microwave power is 800W; the pH was adjusted to 2.0 with 2M HCl; adding pepsin, wherein the mass ratio of the pepsin to the walnut protein is 0.3:100, and stirring for 65min at 37 ℃ with the stirring speed of 80rpm; heating at 95deg.C for 7min, and inactivating enzyme; adjusting the pH value of the solution to 7.0 by using 2M NaOH; centrifuging, collecting supernatant, and lyophilizing to obtain pretreated walnut protein;
(3) Adding a modifier into distilled water, and stirring for 7min at a stirring speed of 80rpm, wherein the mass ratio of the modifier to distilled water is 1:102; adding pretreated walnut protein, and continuously stirring for 113min, wherein the mass ratio of the pretreated walnut protein to the modifier is 1:2.5; freeze drying, and heating in a dryer for 135min at 130deg.C. The modifier is pullulan.
Comparative example 2
The mellow and thick flavored milk black tea comprises the following raw materials in parts by weight: 100 parts of Jinjunmei tea soup, 50 parts of fresh cow milk, 12 parts of white rabbit milk jelly, 8 parts of black pearl, 0.6 part of additive and 2 parts of sucrose.
The preparation method of the mellow and thick flavored milk black tea comprises the following steps of:
step one: 1 part by weight of Jinjunmei black tea is made into a tea bag by gauze, then added into 50 parts by weight of purified water, extracted for 35s by a tea extraction machine at 2000rpm, and then Jinjunmei tea soup is obtained;
step two: adding 20 parts by weight of white rabbit toffee and 3.5 parts by weight of gelatin into 80 parts by weight of fresh cow milk, and stirring for 20min at 67 ℃ at a stirring speed of 50rpm; homogenizing at 5000rpm for 1min; pouring into a mold container for shaping, and refrigerating at 4deg.C for 4 hr to obtain white rabbit milk jelly;
step three: adding 50 parts by weight of fresh cow milk into 100 parts by weight of Jinjunmei tea soup, uniformly mixing, adding 0.6 part by weight of additive and 2 parts by weight of sucrose, uniformly mixing, and finally adding 8 parts by weight of black pearl and 12 parts by weight of white rabbit jelly, and uniformly mixing to obtain the pure and thick flavored cow milk black tea.
The additive is functional walnut protein; the functional walnut protein is prepared by the following method:
(1) Adding defatted walnut powder into distilled water, uniformly mixing, wherein the mass ratio of the defatted walnut powder to the distilled water is 1:24, and regulating the pH value to 11.5 by using 2M NaOH; stirring at 56 ℃ for 12min at a stirring rate of 80rpm; ultrasonic treatment is carried out for 23min, and the ultrasonic frequency is 25kHz; stirring is continued for 70min at 56 ℃, and the stirring speed is 80rpm; centrifuging, collecting supernatant, adjusting pH to 4.3 with 2M HCl, and standing for 75min; centrifuging, filtering, and cleaning the filter residue with distilled water until the pH value of the cleaning liquid is 7.0; freeze drying to obtain walnut protein;
(2) Adding walnut protein into distilled water, and stirring for 100min at a stirring speed of 80rpm, wherein the mass ratio of the walnut protein to the distilled water is 5:100; homogenizing at 5000rpm for 2min, and standing for 50min; the pH was adjusted to 2.0 with 2M HCl; adding pepsin, wherein the mass ratio of the pepsin to the walnut protein is 0.3:100, and stirring for 65min at 37 ℃ with the stirring speed of 80rpm; heating at 95deg.C for 7min, and inactivating enzyme; adjusting the pH value of the solution to 7.0 by using 2M NaOH; centrifuging, collecting supernatant, and lyophilizing to obtain pretreated walnut protein;
(3) Adding a modifier into distilled water, and stirring for 7min at a stirring speed of 80rpm, wherein the mass ratio of the modifier to distilled water is 1:102; adding pretreated walnut protein, and continuously stirring for 113min, wherein the mass ratio of the pretreated walnut protein to the modifier is 1:2.5; freeze drying, and heating in a dryer for 135min at 130deg.C. The modifier is pullulan.
Comparative example 3
The mellow and thick flavored milk black tea comprises the following raw materials in parts by weight: 100 parts of Jinjunmei tea soup, 50 parts of fresh cow milk, 12 parts of white rabbit milk jelly, 8 parts of black pearl, 0.6 part of additive and 2 parts of sucrose.
The preparation method of the mellow and thick flavored milk black tea comprises the following steps of:
step one: 1 part by weight of Jinjunmei black tea is made into a tea bag by gauze, then added into 50 parts by weight of purified water, extracted for 35s by a tea extraction machine at 2000rpm, and then Jinjunmei tea soup is obtained;
step two: adding 20 parts by weight of white rabbit toffee and 3.5 parts by weight of gelatin into 80 parts by weight of fresh cow milk, and stirring for 20min at 67 ℃ at a stirring speed of 50rpm; homogenizing at 5000rpm for 1min; pouring into a mold container for shaping, and refrigerating at 4deg.C for 4 hr to obtain white rabbit milk jelly;
step three: adding 50 parts by weight of fresh cow milk into 100 parts by weight of Jinjunmei tea soup, uniformly mixing, adding 0.6 part by weight of additive and 2 parts by weight of sucrose, uniformly mixing, and finally adding 8 parts by weight of black pearl and 12 parts by weight of white rabbit jelly, and uniformly mixing to obtain the pure and thick flavored cow milk black tea.
The additive is functional walnut protein; the functional walnut protein is prepared by the following method:
(1) Adding defatted walnut powder into distilled water, uniformly mixing, wherein the mass ratio of the defatted walnut powder to the distilled water is 1:24, and regulating the pH value to 11.5 by using 2M NaOH; stirring at 56 ℃ for 12min at a stirring rate of 80rpm; ultrasonic treatment is carried out for 23min, and the ultrasonic frequency is 25kHz; stirring is continued for 70min at 56 ℃, and the stirring speed is 80rpm; centrifuging, collecting supernatant, adjusting pH to 4.3 with 2M HCl, and standing for 75min; centrifuging, filtering, and cleaning the filter residue with distilled water until the pH value of the cleaning liquid is 7.0; freeze drying to obtain walnut protein;
(2) Adding a modifier into distilled water, and stirring for 7min at a stirring speed of 80rpm, wherein the mass ratio of the modifier to distilled water is 1:102; adding walnut protein, and continuing stirring for 113min, wherein the mass ratio of the walnut protein to the modifier is 1:2.5; freeze drying, and heating in a dryer for 135min at 130deg.C. The modifier is pullulan.
Comparative example 4
The mellow and thick flavored milk black tea comprises the following raw materials in parts by weight: 100 parts of Jinjunmei tea soup, 50 parts of fresh cow milk, 12 parts of white rabbit milk jelly, 8 parts of black pearl, 0.6 part of additive and 2 parts of sucrose.
The preparation method of the mellow and thick flavored milk black tea comprises the following steps of:
Step one: 1 part by weight of Jinjunmei black tea is made into a tea bag by gauze, then added into 50 parts by weight of purified water, extracted for 35s by a tea extraction machine at 2000rpm, and then Jinjunmei tea soup is obtained;
step two: adding 20 parts by weight of white rabbit toffee and 3.5 parts by weight of gelatin into 80 parts by weight of fresh cow milk, and stirring for 20min at 67 ℃ at a stirring speed of 50rpm; homogenizing at 5000rpm for 1min; pouring into a mold container for shaping, and refrigerating at 4deg.C for 4 hr to obtain white rabbit milk jelly;
step three: adding 50 parts by weight of fresh cow milk into 100 parts by weight of Jinjunmei tea soup, uniformly mixing, adding 0.6 part by weight of additive and 2 parts by weight of sucrose, uniformly mixing, and finally adding 8 parts by weight of black pearl and 12 parts by weight of white rabbit jelly, and uniformly mixing to obtain the pure and thick flavored cow milk black tea.
The additive is functional walnut protein; the functional walnut protein is prepared by the following method:
(1) Adding defatted walnut powder into distilled water, uniformly mixing, wherein the mass ratio of the defatted walnut powder to the distilled water is 1:24, and regulating the pH value to 11.5 by using 2M NaOH; stirring at 56 ℃ for 12min at a stirring rate of 80rpm; ultrasonic treatment is carried out for 23min, and the ultrasonic frequency is 25kHz; stirring is continued for 70min at 56 ℃, and the stirring speed is 80rpm; centrifuging, collecting supernatant, adjusting pH to 4.3 with 2M HCl, and standing for 75min; centrifuging, filtering, and cleaning the filter residue with distilled water until the pH value of the cleaning liquid is 7.0; freeze drying to obtain walnut protein;
(2) Adding walnut protein into distilled water, and stirring for 100min at a stirring speed of 80rpm, wherein the mass ratio of the walnut protein to the distilled water is 5:100; homogenizing at 5000rpm for 2min, and standing for 50min; performing microwave treatment for 3min, wherein the microwave power is 800W; the pH was adjusted to 2.0 with 2M HCl; adding pepsin, wherein the mass ratio of the pepsin to the walnut protein is 0.3:100, and stirring for 65min at 37 ℃ with the stirring speed of 80rpm; heating at 95deg.C for 7min, and inactivating enzyme; adjusting the pH value of the solution to 7.0 by using 2M NaOH; centrifuging, collecting supernatant, and lyophilizing to obtain functional walnut protein.
Comparative example 5
The mellow and thick flavored milk black tea comprises the following raw materials in parts by weight: 100 parts of Jinjunmei tea soup, 50 parts of fresh cow milk, 12 parts of white rabbit milk jelly, 8 parts of black pearl, 0.6 part of additive and 2 parts of sucrose.
The preparation method of the mellow and thick flavored milk black tea comprises the following steps of:
step one: 1 part by weight of Jinjunmei black tea is made into a tea bag by gauze, then added into 50 parts by weight of purified water, extracted for 35s by a tea extraction machine at 2000rpm, and then Jinjunmei tea soup is obtained;
step two: adding 20 parts by weight of white rabbit toffee and 3.5 parts by weight of gelatin into 80 parts by weight of fresh cow milk, and stirring for 20min at 67 ℃ at a stirring speed of 50rpm; homogenizing at 5000rpm for 1min; pouring into a mold container for shaping, and refrigerating at 4deg.C for 4 hr to obtain white rabbit milk jelly;
Step three: adding 50 parts by weight of fresh cow milk into 100 parts by weight of Jinjunmei tea soup, uniformly mixing, adding 0.6 part by weight of additive and 2 parts by weight of sucrose, uniformly mixing, and finally adding 8 parts by weight of black pearl and 12 parts by weight of white rabbit jelly, and uniformly mixing to obtain the pure and thick flavored cow milk black tea.
The additive is walnut protein; the walnut protein is prepared by the following method:
adding defatted walnut powder into distilled water, uniformly mixing, wherein the mass ratio of the defatted walnut powder to the distilled water is 1:24, and regulating the pH value to 11.5 by using 2M NaOH; stirring at 56 ℃ for 12min at a stirring rate of 80rpm; ultrasonic treatment is carried out for 23min, and the ultrasonic frequency is 25kHz; stirring is continued for 70min at 56 ℃, and the stirring speed is 80rpm; centrifuging, collecting supernatant, adjusting pH to 4.3 with 2M HCl, and standing for 75min; centrifuging, filtering, and cleaning the filter residue with distilled water until the pH value of the cleaning liquid is 7.0; and (5) freeze-drying to obtain the walnut protein.
Comparative example 6
The mellow and thick flavored milk black tea comprises the following raw materials in parts by weight: 100 parts of Jinjunmei tea soup, 50 parts of fresh cow milk, 12 parts of white rabbit milk jelly, 8 parts of black pearl, 0.6 part of additive and 2 parts of sucrose.
The preparation method of the mellow and thick flavored milk black tea comprises the following steps of:
step one: 1 part by weight of Jinjunmei black tea is made into a tea bag by gauze, then added into 50 parts by weight of purified water, extracted for 35s by a tea extraction machine at 2000rpm, and then Jinjunmei tea soup is obtained;
step two: adding 20 parts by weight of white rabbit toffee and 3.5 parts by weight of gelatin into 80 parts by weight of fresh cow milk, and stirring for 20min at 67 ℃ at a stirring speed of 50rpm; homogenizing at 5000rpm for 1min; pouring into a mold container for shaping, and refrigerating at 4deg.C for 4 hr to obtain white rabbit milk jelly;
step three: adding 50 parts by weight of fresh cow milk into 100 parts by weight of Jinjunmei tea soup, uniformly mixing, adding 0.6 part by weight of additive and 2 parts by weight of sucrose, uniformly mixing, and finally adding 8 parts by weight of black pearl and 12 parts by weight of white rabbit jelly, and uniformly mixing to obtain the pure and thick flavored cow milk black tea. The additive is sodium caseinate; wherein, sodium caseinate, food grade, CAS:9005-46-3, purity: 98%, purchased from Hubei Xinrun chemical Co., ltd.
Comparative example 7
The mellow and thick flavored milk black tea comprises the following raw materials in parts by weight: 100 parts of Jinjunmei tea soup, 50 parts of fresh cow milk, 12 parts of white rabbit milk jelly, 8 parts of black pearl, 0.6 part of additive and 2 parts of sucrose.
The preparation method of the mellow and thick flavored milk black tea comprises the following steps of:
step one: 1 part by weight of Jinjunmei black tea is made into a tea bag by gauze, then added into 50 parts by weight of purified water, extracted for 35s by a tea extraction machine at 2000rpm, and then Jinjunmei tea soup is obtained;
step two: adding 20 parts by weight of white rabbit toffee and 3.5 parts by weight of gelatin into 80 parts by weight of fresh cow milk, and stirring for 20min at 67 ℃ at a stirring speed of 50rpm; homogenizing at 5000rpm for 1min; pouring into a mold container for shaping, and refrigerating at 4deg.C for 4 hr to obtain white rabbit milk jelly;
step three: adding 50 parts by weight of fresh cow milk into 100 parts by weight of Jinjunmei tea soup, uniformly mixing, adding 0.6 part by weight of additive and 2 parts by weight of sucrose, uniformly mixing, and finally adding 8 parts by weight of black pearl and 12 parts by weight of white rabbit jelly, and uniformly mixing to obtain the pure and thick flavored cow milk black tea.
The additive is functional walnut protein; the functional walnut protein is prepared by the following method:
(1) Adding defatted walnut powder into distilled water, uniformly mixing, wherein the mass ratio of the defatted walnut powder to the distilled water is 1:24, and regulating the pH value to 11.5 by using 2M NaOH; stirring at 56 ℃ for 12min at a stirring rate of 80rpm; ultrasonic treatment is carried out for 23min, and the ultrasonic frequency is 25kHz; stirring is continued for 70min at 56 ℃, and the stirring speed is 80rpm; centrifuging, collecting supernatant, adjusting pH to 4.3 with 2M HCl, and standing for 75min; centrifuging, filtering, and cleaning the filter residue with distilled water until the pH value of the cleaning liquid is 7.0; freeze drying to obtain walnut protein;
(2) Adding walnut protein into distilled water, and stirring for 100min at a stirring speed of 80rpm, wherein the mass ratio of the walnut protein to the distilled water is 5:100; homogenizing at 5000rpm for 2min, and standing for 50min; performing microwave treatment for 3min, wherein the microwave power is 800W; the pH was adjusted to 2.0 with 2M HCl; adding pepsin, wherein the mass ratio of the pepsin to the walnut protein is 0.3:100, and stirring for 65min at 37 ℃ with the stirring speed of 80rpm; heating at 95deg.C for 7min, and inactivating enzyme; adjusting the pH value of the solution to 7.0 by using 2M NaOH; centrifuging, collecting supernatant, and lyophilizing to obtain pretreated walnut protein;
(3) Adding a modifier into distilled water, and stirring for 7min at a stirring speed of 80rpm, wherein the mass ratio of the modifier to distilled water is 1:102; adding pretreated walnut protein, and continuously stirring for 113min, wherein the mass ratio of the pretreated walnut protein to the modifier is 1:2.5; freeze drying, and heating in a dryer for 135min at 130deg.C. The modifier is glucan. Wherein, dextran, food grade, CAS:9004-54-0, 40kDa, available from Shanghai Beian Biotech Co.
Example 5
The mellow and thick flavored milk black tea comprises the following raw materials in parts by weight: 100 parts of Jinjunmei tea soup, 50 parts of fresh cow milk, 12 parts of white rabbit milk jelly, 8 parts of black pearl, 0.6 part of additive and 2 parts of sucrose.
The preparation method of the mellow and thick flavored milk black tea comprises the following steps of:
step one: 1 part by weight of Jinjunmei black tea is made into a tea bag by gauze, then added into 50 parts by weight of purified water, extracted for 35s by a tea extraction machine at 2000rpm, and then Jinjunmei tea soup is obtained;
step two: adding 20 parts by weight of white rabbit toffee and 3.5 parts by weight of gelatin into 80 parts by weight of fresh cow milk, and stirring for 20min at 67 ℃ at a stirring speed of 50rpm; homogenizing at 5000rpm for 1min; pouring into a mold container for shaping, and refrigerating at 4deg.C for 4 hr to obtain white rabbit milk jelly;
step three: adding 50 parts by weight of fresh cow milk into 100 parts by weight of Jinjunmei tea soup, uniformly mixing, adding 0.6 part by weight of additive and 2 parts by weight of sucrose, uniformly mixing, and finally adding 8 parts by weight of black pearl and 12 parts by weight of white rabbit jelly, and uniformly mixing to obtain the pure and thick flavored cow milk black tea.
The additive is functional walnut protein; the functional walnut protein is prepared by the following method:
(1) Adding defatted walnut powder into distilled water, uniformly mixing, wherein the mass ratio of the defatted walnut powder to the distilled water is 1:24, and regulating the pH value to 11.5 by using 2M NaOH; stirring at 56 ℃ for 12min at a stirring rate of 80rpm; ultrasonic treatment is carried out for 23min, and the ultrasonic frequency is 25kHz; stirring is continued for 70min at 56 ℃, and the stirring speed is 80rpm; centrifuging, collecting supernatant, adjusting pH to 4.3 with 2M HCl, and standing for 75min; centrifuging, filtering, and cleaning the filter residue with distilled water until the pH value of the cleaning liquid is 7.0; freeze drying to obtain walnut protein;
(2) Adding walnut protein into distilled water, and stirring for 100min at a stirring speed of 80rpm, wherein the mass ratio of the walnut protein to the distilled water is 5:100; homogenizing at 5000rpm for 2min, and standing for 50min; performing microwave treatment for 3min, wherein the microwave power is 800W; the pH was adjusted to 2.0 with 2M HCl; adding pepsin, wherein the mass ratio of the pepsin to the walnut protein is 0.3:100, and stirring for 65min at 37 ℃ with the stirring speed of 80rpm; heating at 95deg.C for 7min, and inactivating enzyme; adjusting the pH value of the solution to 7.0 by using 2M NaOH; centrifuging, collecting supernatant, and lyophilizing to obtain pretreated walnut protein;
(3) Adding a modifier into distilled water, and stirring for 7min at a stirring speed of 80rpm, wherein the mass ratio of the modifier to distilled water is 1:102; adding pretreated walnut protein, and continuously stirring for 113min, wherein the mass ratio of the pretreated walnut protein to the modifier is 1:2.5; freeze drying, and heating in a dryer for 135min at 130deg.C. The modifier is fucoidin.
Example 6
The mellow and thick flavored milk black tea comprises the following raw materials in parts by weight: 100 parts of Jinjunmei tea soup, 50 parts of fresh cow milk, 12 parts of white rabbit milk jelly, 8 parts of black pearl, 0.6 part of additive and 2 parts of sucrose.
The preparation method of the mellow and thick flavored milk black tea comprises the following steps of:
step one: 1 part by weight of Jinjunmei black tea is made into a tea bag by gauze, then added into 50 parts by weight of purified water, extracted for 35s by a tea extraction machine at 2000rpm, and then Jinjunmei tea soup is obtained;
step two: adding 20 parts by weight of white rabbit toffee and 3.5 parts by weight of gelatin into 80 parts by weight of fresh cow milk, and stirring for 20min at 67 ℃ at a stirring speed of 50rpm; homogenizing at 5000rpm for 1min; pouring into a mold container for shaping, and refrigerating at 4deg.C for 4 hr to obtain white rabbit milk jelly;
step three: adding 50 parts by weight of fresh cow milk into 100 parts by weight of Jinjunmei tea soup, uniformly mixing, adding 0.6 part by weight of additive and 2 parts by weight of sucrose, uniformly mixing, and finally adding 8 parts by weight of black pearl and 12 parts by weight of white rabbit jelly, and uniformly mixing to obtain the pure and thick flavored cow milk black tea.
The additive is functional walnut protein; the functional walnut protein is prepared by the following method:
(1) Adding defatted walnut powder into distilled water, uniformly mixing, wherein the mass ratio of the defatted walnut powder to the distilled water is 1:24, and regulating the pH value to 11.5 by using 2M NaOH; stirring at 56 ℃ for 12min at a stirring rate of 80rpm; ultrasonic treatment is carried out for 23min, and the ultrasonic frequency is 25kHz; stirring is continued for 70min at 56 ℃, and the stirring speed is 80rpm; centrifuging, collecting supernatant, adjusting pH to 4.3 with 2M HCl, and standing for 75min; centrifuging, filtering, and cleaning the filter residue with distilled water until the pH value of the cleaning liquid is 7.0; freeze drying to obtain walnut protein;
(2) Adding walnut protein into distilled water, and stirring for 100min at a stirring speed of 80rpm, wherein the mass ratio of the walnut protein to the distilled water is 5:100; homogenizing at 5000rpm for 2min, and standing for 50min; performing microwave treatment for 3min, wherein the microwave power is 800W; the pH was adjusted to 2.0 with 2M HCl; adding pepsin, wherein the mass ratio of the pepsin to the walnut protein is 0.3:100, and stirring for 65min at 37 ℃ with the stirring speed of 80rpm; heating at 95deg.C for 7min, and inactivating enzyme; adjusting the pH value of the solution to 7.0 by using 2M NaOH; centrifuging, collecting supernatant, and lyophilizing to obtain pretreated walnut protein;
(3) Adding a modifier into distilled water, and stirring for 7min at a stirring speed of 80rpm, wherein the mass ratio of the modifier to distilled water is 1:102; adding pretreated walnut protein, and continuously stirring for 113min, wherein the mass ratio of the pretreated walnut protein to the modifier is 1:2.5; freeze drying, and heating in a dryer for 135min at 130deg.C. The modifier is a mixture of fucoidin and pullulan according to a mass ratio of 4:5.
Test example 2
Emulsion stability determination: the additives described in examples 4-6 and comparative examples 1-7 were tested with a slight modification to the Pearceand and Kinsella procedure. Mixing 15mL of additive solution with 5mL of soybean oil, homogenizing at 5000rpm for 2min to obtain emulsion, and standing; 50-fold dilution was performed with SDS solution (0.1 wt%) and absorbance (500 nm) was measured at rest for 0min and 10 min. Wherein the additive solution is a 1wt% additive solution prepared by using a PBS solution (ph= 7,0.2M), and an SDS solution (0.1 wt%) is used as a blank; parallel 5 groups, average.
Emulsion stability = OD 1/(OD 1-OD 2) ×Δt
Wherein OD1 is absorbance at rest for 0min, OD2 is absorbance at rest for 10min, and DeltaT is absorbance time interval measured twice.
Table 2 emulsion stability test of additives
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Test example 3
Determining the effect of the additive on the organoleptic quality of the cow's milk black tea: 10 professionally trained individuals were selected, 5 men and 5 women, to evaluate the impact of the additives described in examples 4-6 and comparative examples 3-7 on the organoleptic quality of the black tea of cow's milk; 1 part by weight of Jinjunmei black tea is made into a tea bag by gauze, then added into 50 parts by weight of purified water, extracted for 35s by a tea extraction machine at 2000rpm, and then Jinjunmei tea soup is obtained; and adding 50 parts by weight of fresh cow milk into 100 parts by weight of Jinjunmei tea soup, uniformly mixing, adding 0.6 part by weight of the additive described in examples 4-6 and comparative examples 3-7 and 2 parts by weight of sucrose, and uniformly mixing to obtain cow milk black tea. Wherein the fresh cow milk is commercial pasteurized fresh cow milk. The full scale was 100 minutes and the test results averaged. The blank is without any additives.
TABLE 3 sensory quality scoring criteria
Table 4 effect of additives on organoleptic quality of cow's milk black tea (score)
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Comprehensive scoring
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Example 4
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96.5
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Example 5
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96.0
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Example 6
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98.2
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Comparative example 3
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92.8
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Comparative example 4
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93.6
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Comparative example 5
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89.5
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Comparative example 6
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92.1
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Comparative example 7
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94.3
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Blank group
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87.9 |
Comparing example 4 with comparative examples 5-6, the present invention adds functionalized walnut protein as an additive; the functional walnut protein is not only a nutrition additive for providing rich beneficial essential amino acids for human bodies, but also a functional additive, can improve the sensory quality of milk tea, and effectively improve the taste, smell, color and state of the milk tea.
Test example 4
Determining the effect of the additive on the stability of the cow's milk black tea: 1 part by weight of Jinjunmei black tea is made into a tea bag by gauze, then added into 50 parts by weight of purified water, extracted for 35s by a tea extraction machine at 2000rpm, and then Jinjunmei tea soup is obtained; and adding 50 parts by weight of fresh cow milk into 100 parts by weight of Jinjunmei tea soup, uniformly mixing, adding 0.6 part by weight of the additive described in examples 4-6 and comparative examples 3-7 and 2 parts by weight of sucrose, and uniformly mixing to obtain cow milk black tea. Stability was evaluated by measuring the oil precipitation rate of the above-mentioned cow's milk black tea. The above milk black tea was centrifuged at 3000r/min for 10min and averaged over 5 groups. The blank is without any additives.
Fat precipitation rate (%) =x1/x2×100
Wherein the height of the X1 top floating layer is mm; x2 height of sample liquid, mm.
TABLE 5 Effect of additives on stability of cow's milk black tea
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As can be seen from comparison between example 4 and comparative examples 3-5, in the preparation process of the functionalized walnut protein, the interfacial adsorption capacity of the walnut protein is increased by pepsin treatment, which is beneficial to improving the stability of an emulsion system; the modifier is combined with the surface adsorption layer of the walnut protein through electrostatic action and covalent action to form functional walnut protein; in the emulsion system, the protein structure of the functionalized walnut protein acts with the oil phase, and the branched chain structure of the modifier acts with the water phase, so that the steric hindrance between the emulsions is further increased, and the stability of the emulsion system is maintained.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the invention.