CN112029687A

CN112029687A - Horikoshi's cell bacillus strain and application thereof

Info

Publication number: CN112029687A
Application number: CN202010951334.2A
Authority: CN
Inventors: 刘侃诚
Original assignee: Senjing Bioengineering Huzhou Co ltd
Current assignee: Zhejiang senjing Agricultural Biotechnology Co.,Ltd.
Priority date: 2020-09-10
Filing date: 2020-09-10
Publication date: 2020-12-04

Abstract

The invention relates to a Horikoshi's cell bacillus strain and application thereof, wherein the preservation number of the bacterial strain Hornbaishi's cell bacillus (Cytobacillus horneckiae) is CGMCC No.20452, and the 16s rDNA base sequence of the bacterial strain is shown as SEQ ID NO: 1 is shown. The invention also relates to a method for planting the strain, which comprises the following specific steps: (1) a fermentation medium formula; (2) fermentation processes (including fermentation parameters and methods); (3) a fermentation liquor planting application method; the invention has the advantages that the bacterial strains colonize in soil and occupy the advantage of the zone, the propagation of pathogenic bacteria is inhibited, the soil nutrient condition is improved, and the growth of crops is promoted.

Description

Horikoshi's cell bacillus strain and application thereof

Technical Field

The invention relates to a bacterial strain and application thereof, in particular to a horiba cytobacterium strain and application thereof.

Background

At present, China is a large chemical fertilizer producing country and a large pesticide producing country in the world, and the mode of chemical fertilizer, pesticide and ship protection creates grains in China and increases the yield in successive years, and brings a series of major problems of soil quality reduction, serious pest and disease damage, quality reduction of agricultural products, water pollution, even safety of the agricultural products and the like. The existing agricultural extensive operation mode is an unsustainable development mode, and a chemical agricultural production mode needs to be converted into an ecological agricultural production mode in the future.

Agricultural microbial agents for use in the growing industry are often cultured from bacteria, actinomycetes, fungi and yeasts that are effective and safe for plants. Agricultural microbial agents for use in the crop production have different functions and uses due to differences in the species or combination of species. The method comprises the steps of soil micro-ecological balance, pest and disease prevention, soil nutrient improvement, plant growth promotion and the like. However, at present, microbial products which can be widely used and have excellent performance are still relatively lacking.

Disclosure of Invention

It is an object of the present invention to solve the above-mentioned deficiencies of the prior art and to provide a horiba cytobacterium.

The second objective of the present invention is to solve the above-mentioned deficiencies of the prior art, and to provide a use of horiba cytobacterium (CH-005) in soil, wherein the horiba cytobacterium (CH-005) is rapidly propagated by using the nutrient substances in the soil and becomes the dominant flora in the soil, thereby inhibiting the propagation of pathogenic bacteria, improving the soil nutrition and physicochemical conditions, and promoting the production of crops, so as to achieve the purpose of green planting.

In order to achieve the purpose, the invention adopts the following technical scheme:

a Horikoshi's Cytobacillus (Cytobacillus horneckiae) strain, the code of the strain is CH-005, the preservation number is CGMCC No.20452, the preservation unit is China general microbiological culture Collection center (CGMCC), the preservation unit address is: the microbial research institute of the national academy of sciences No. 3, Xilu No. 1, Beijing, Chaoyang, and the preservation date is 07 months and 27 days in 2020.

The strain is subjected to physiological and biochemical tests and 16rDNA sequence determination, the strain CH-005 is Horikoshi's Cytobacillus (Cytobacillus horneckiae), and the 16s rDNA base sequence of the strain is shown as SEQ ID NO: 1 is shown.

The invention also discloses a method for applying the horiba horikoshii cytobacter strain to the planting industry, which comprises the following steps:

1. preparing a culture medium: 1% (g/ml) of sucrose, 1% (g/ml) of yeast extract, 0.5% (g/ml) of sodium chloride, supplementing water, and sterilizing for later use;

2. liquid submerged fermentation culture: inoculating the single bacterial colony of the strain into the culture medium, performing shake culture at 120r/min, wherein the fermentation temperature is 40 ℃, the fermentation time is 32 hours, and the OD value of the fermentation liquor is 6;

3. the application of promoting the growth of crops: applying the fermentation liquid of the horiba-shimeji cytobacillus into soil in a base fertilizer or top dressing mode, wherein the usage amount of the fermentation liquid per mu of land is 25 kg.

The invention has the beneficial effects that: the strain CH-005 is an environmental microorganism, has strong adaptability and good safety, can be quickly propagated in soil and occupy the advantage of a region for a long time, can widely inhibit the propagation of pathogenic bacteria, avoids the accumulation of harmful metabolites and effectively promotes the growth of crops, thereby achieving the aim of green planting.

Drawings

FIG. 1 is a diagram: horikoshi's cytobacillus CGMCC No.20452 rice growth promoting effect map.

Detailed Description

The technical scheme of the invention is further illustrated by the following specific examples, wherein the reagents and raw materials used in the examples are all commercially available conventional raw materials.

Example 1:

application of Horikoshi's cytobacterium:

a. preparing a culture medium: 1% (g/ml) of sucrose, 1% (g/ml) of yeast extract, 0.5% (g/ml) of sodium chloride, supplementing water, and autoclaving for use;

b. liquid submerged fermentation culture: inoculating the single bacterial colony of the strain into the culture medium, performing shake culture at 120r/min, wherein the fermentation temperature is 40 ℃, the fermentation time is 32 hours, and the OD value of the fermentation liquor is 6;

c.2019, performing a rice growth promotion test in a standard planting plot of Chinese Rice research institute in Fuyang district, Hangzhou, Zhejiang, applying fermentation broth of Horikoshi's cytobacilli into soil in the form of base fertilizer, wherein the fermentation broth is used at 25kg per mu of land, transplanting (seedling variety: Youhou 12) is started one week after application, uniformly harvesting rice after the rice is mature, counting the yield per mu, normally managing the fertilizer water and plant diseases and insect pests during the period, and not applying the fermentation broth of Horikoshi's cytobacilli in a control group;

d. the experimental results are shown in figure 1. From FIG. 1, it is evident that the yield of the Horikoshi's cell bacillus CGMCC No.20452 strain treated group is increased significantly, the yield of the control group is 552.0 kg/mu, the yield of the treated group is 633.4 kg/mu, and the yield is increased by 14.7% per mu.

The above-described embodiments are only preferred embodiments of the present invention, and are not intended to limit the present invention in any way, and other variations and modifications may be made without departing from the spirit of the invention as set forth in the claims.

Sequence listing

<110> Senzo bioengineering (Huzhou) Co., Ltd

<120> a Horikoshi's cell bacillus strain and its use

<130> 2020

<160> 1

<170> SIPOSequenceListing 1.0

<210> 1

<211> 1480

<212> DNA

<213> Bacillus horikoshii cell (Cytobacillus horneckiae)

<400> 1

tttaagtttt ggaagtgatc atggctcagg acgaacgctg gcggcgtgcc taatacatgc 60

aagtcgagcg aatctgaggg agcttgctcc caaagattag cggcggacgg gtgagtaaca 120

cgtgggcaac ctgcctgtaa gactgggata acttcgggaa accggagcta ataccggata 180

atccctttcc tcacatgagg aaaggctgaa agacggcgtt tagctgtcac ttacagatgg 240

gcccgcggcg cattagctag ttggtgaggt aacggctcac caaggccacg atgcgtagcc 300

gacctgagag ggtgatcggc cacactggga ctgagacacg gcccagactc ctacgggagg 360

cagcagtagg gaatcttccg caatggacga aagtctgacg gagcaacgcc gcgtgagtga 420

tgaaggtttt cggatcgtaa aactctgttg ttagggaaga acaagtatcg gagtaactgc 480

cggtaccttg acggtaccta accagaaagc cacggctaac tacgtgccag cagccgcggt 540

aatacgtagg tggcaagcgt tgtccggaat tattgggcgt aaagcgcgcg caggtggttc 600

cttaagtctg atgtgaaagc ccacggctca accgtggagg gtcattggaa actggggaac 660

ttgagtgcag aagaggaaag tggaattcca agtgtagcgg tgaaatgcgt agagatttgg 720

aggaacacca gtggcgaagg cgactttctg gtctgtaact gacactgagg cgcgaaagcg 780

tggggagcga acaggattag ataccctggt agtccacgcc gtaaacgatg agtgctaagt 840

gttagagggt ttccgccctt tagtgctgca gcaaacgcat taagcactcc gcctggggag 900

tacgaccgca aggttgaaac tcaaaggaat tgacgggggc ccgcacaagc ggtggagcat 960

gtggtttaat tcgaagcaac gcgaagaacc ttaccaggtc ttgacatcct ctgacaatcc 1020

tagagatagg acgttcccct tcgggggaca gagtgacagg tggtgcatgg ttgtcgtcag 1080

ctcgtgtcgt gagatgttgg gttaagtccc gcaacgagcg caacccttga tcttagttgc 1140

cagcattcag ttgggcactc taaggtgact gccggtgaca aaccggagga aggtggggat 1200

gacgtcaaat catcatgccc cttatgacct gggctacaca cgtgctacaa tggatggtac 1260

aaagggctgc aagaccgcga ggtttagcca atcccataaa accattctca gttcggattg 1320

caggctgcaa ctcgcctgca tgaagccgga atcgctagta atcgcggatc agcatgccgc 1380

ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accacgagag tttgtaacac 1440

ccgaagtcgg tggggtaacc ttttggagcc agccgcctaa 1480

Claims

1. The Horikoshi's cytobacterium strain is characterized in that the code of the strain is CH-005, the preservation number is CGMCC No.20452, the preservation unit is the China general microbiological culture Collection center, and the preservation unit address is as follows: the microbial research institute of the national academy of sciences No. 3, Xilu No. 1, Beijing, Chaoyang, and the preservation date is 07 months and 27 days in 2020.

2. The use of the Horikoshi's cell Bacillus strain of claim 1 for promoting the growth of a plant.

3. The use of claim 2, wherein the fermentation broth of horiba cytobacter horikoshii is applied to the soil in the form of a base fertilizer or a top dressing, wherein the amount of the fermentation broth is 25kg per mu of land.

4. The use of claim 3, wherein the Horikoshi's cell bacillus fermentation broth is cultured by the following method:

(1) the formula of the culture medium is as follows: 1% of sucrose, 1% of yeast extract and 0.5% of sodium chloride, supplementing water, and sterilizing for later use;

(2) liquid submerged fermentation culture: and inoculating the single bacterial colony of the strain into the culture medium, performing shaking culture at 120r/min, wherein the fermentation temperature is 40 ℃, the fermentation time is 32 hours, and the OD value of the fermentation liquid is 6.