CN112021556A - Artemisia argyi enzyme and preparation method thereof - Google Patents
Artemisia argyi enzyme and preparation method thereof Download PDFInfo
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- CN112021556A CN112021556A CN202010864114.6A CN202010864114A CN112021556A CN 112021556 A CN112021556 A CN 112021556A CN 202010864114 A CN202010864114 A CN 202010864114A CN 112021556 A CN112021556 A CN 112021556A
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- 102000004190 Enzymes Human genes 0.000 title claims abstract description 64
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 64
- 235000010894 Artemisia argyi Nutrition 0.000 title claims abstract description 11
- 238000002360 preparation method Methods 0.000 title abstract description 15
- 241001435059 Artemisia argyi Species 0.000 title description 2
- 235000003261 Artemisia vulgaris Nutrition 0.000 claims abstract description 68
- 240000006891 Artemisia vulgaris Species 0.000 claims abstract description 64
- 235000009051 Ambrosia paniculata var. peruviana Nutrition 0.000 claims abstract description 58
- 235000003097 Artemisia absinthium Nutrition 0.000 claims abstract description 58
- 235000017731 Artemisia dracunculus ssp. dracunculus Nutrition 0.000 claims abstract description 58
- 239000001138 artemisia absinthium Substances 0.000 claims abstract description 58
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 claims abstract description 58
- 238000000855 fermentation Methods 0.000 claims abstract description 48
- 230000004151 fermentation Effects 0.000 claims abstract description 48
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 42
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 21
- 239000000203 mixture Substances 0.000 claims abstract description 18
- 238000004140 cleaning Methods 0.000 claims abstract description 12
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- 240000000111 Saccharum officinarum Species 0.000 claims abstract description 6
- 239000012535 impurity Substances 0.000 claims abstract description 6
- 238000002156 mixing Methods 0.000 claims abstract description 6
- 238000002791 soaking Methods 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims description 32
- 238000000034 method Methods 0.000 claims description 14
- 238000005507 spraying Methods 0.000 claims description 11
- 230000003203 everyday effect Effects 0.000 claims description 10
- 238000001914 filtration Methods 0.000 claims description 8
- 239000007787 solid Substances 0.000 claims description 8
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- 229910052760 oxygen Inorganic materials 0.000 claims description 5
- 239000001301 oxygen Substances 0.000 claims description 5
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/10—Ultra-violet radiation
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses a wormwood enzyme and a preparation method thereof, and relates to the technical field of enzymes, wherein the wormwood enzyme comprises the following components in parts by weight: 1-2 parts of wormwood, 1 part of brown sugar and 10 parts of water, and the preparation method comprises the following steps: selecting ecologically planted Chinese mugwort, brown sugar decocted by ecologically planted sugarcane and clear water after high-temperature disinfection; cleaning: soaking fresh folium Artemisiae Argyi in clear water for 20 min, cleaning the surface of the soaked folium Artemisiae Argyi, and removing impurities on the surface of folium Artemisiae Argyi to obtain clean folium Artemisiae Argyi; fermentation: then mixing clean folium artemisiae argyi with brown sugar and water in proportion, and then putting the mixture into a sealed container for fermentation operation; according to the invention, the folium artemisiae argyi enzyme prepared from the folium artemisiae argyi, the brown sugar and the water in a ratio can efficiently sterilize the environment, and the raw materials for preparing the folium artemisiae argyi enzyme are nontoxic, so that the folium artemisiae argyi enzyme can sterilize water, food and air, and the application range of the folium artemisiae argyi enzyme is greatly improved.
Description
Technical Field
The invention belongs to the technical field of enzymes, and particularly relates to a wormwood enzyme and a preparation method thereof.
Background
The ferment is a product containing specific bioactive components and prepared by fermenting animals, plants, fungi and the like serving as raw materials through microorganisms.
The wormwood enzymes and the preparation method in the current market still have defects in application, for example, in the preparation process of the wormwood enzymes, the selection and the proportion of raw materials are difficult to make the effect of the prepared wormwood enzymes fully exerted, in addition, in the preparation process, the added auxiliary materials are easy to influence the effect of the wormwood enzymes and generate side effects easily, so that the application range of the wormwood enzymes is reduced, and the problem of resource waste phenomenon is also easy to occur.
Disclosure of Invention
The present invention aims to provide a wormwood enzyme and a preparation method thereof, so as to solve the problems in the background art.
The technical scheme adopted by the invention is as follows:
the wormwood enzyme comprises the following components in parts by weight: 0.2-2.8 parts of wormwood, 0.5-1.5 parts of brown sugar and 5-18 parts of water.
Preferably, the composition and the parts by weight are as follows: 0.7-2.3 parts of wormwood, 0.7-1.3 parts of brown sugar and 8-15 parts of water.
Preferably, the composition and the parts by weight are as follows: 1-2 parts of wormwood, 1 part of brown sugar and 10 parts of water.
Preferably, the brown sugar may be replaced by molasses.
The preparation method of the wormwood enzyme comprises the following steps:
selecting materials: selecting ecologically planted Chinese mugwort, brown sugar decocted by ecologically planted sugarcane and clear water after high-temperature disinfection;
cleaning: soaking fresh folium Artemisiae Argyi in clear water for 20 min, cleaning the surface of the soaked folium Artemisiae Argyi, and removing impurities on the surface of folium Artemisiae Argyi to obtain clean folium Artemisiae Argyi;
fermentation: then mixing clean folium artemisiae argyi with brown sugar and water in proportion, and then putting the mixture into a sealed container for fermentation operation;
and (3) filtering: after the fermentation finishes, utilize the filter screen to filter the zymotic fluid in the container, the liquid composition in the zymotic fluid is chinese mugwort grass ferment liquid, carries out the splendid attire with the watering can, and the solid component in will fermenting is held with another container splendid attire:
and (3) storing: and (5) storing the spraying pot containing the wormwood enzyme liquid in a room temperature environment.
Preferably, in the fermentation process, the container needs to be subjected to ultraviolet disinfection treatment, and in the fermentation process, the container is located in a dark environment with the temperature of 30-35 ℃ and the oxygen content being low, and the fermentation time is 6 months.
Preferably, the folium artemisiae argyi, the brown sugar and the water are shaken every day in the first month or the container is opened every day to stir the mixture of the folium artemisiae argyi, the brown sugar and the water in the container fermentation process.
Preferably, in the storage process, the watering can containing the wormwood enzyme liquid is prevented from being irradiated by sunlight.
In summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:
1. according to the invention, the folium artemisiae argyi enzyme prepared from the folium artemisiae argyi, the brown sugar and the water in a ratio can efficiently sterilize the environment, and the raw materials for preparing the folium artemisiae argyi enzyme are nontoxic, so that the folium artemisiae argyi enzyme can sterilize water, food and air, and the application range of the folium artemisiae argyi enzyme is greatly improved.
2. According to the invention, the natural property of wormwood, namely extreme yang property, pure yang property, body resistance strengthening, pathogenic factor eliminating, virus killing, the inherent functions and advantages of dredging twelve meridians and collaterals of a human body to regulate and treat various diseases are utilized, and the principle of sterilization, inflammation diminishing and environment purification of enzyme is combined to ferment the wormwood enzyme, so that the wormwood enzyme can combine the energy for improving immunity and the biological purification energy of environment-friendly enzyme, and further has great effects on epidemic prevention, sterilization, inflammation diminishing, environment purification and human body immunity improvement.
3. According to the invention, the fermentation liquor is filtered by using the filter screen, so that the wormwood enzyme liquid and the prepared wormwood enzyme liquid solid raw materials can be separated, and further the wormwood enzyme liquid and the wormwood enzyme liquid solid raw materials can be respectively applied to different fields, and the utilization of resources is improved.
4. According to the invention, the container is shaken in the first month of the fermentation process of the wormwood enzyme liquid, so that raw materials for preparing the wormwood enzyme liquid can be fully contacted, the efficiency of the wormwood enzyme liquid preparation is improved, and the quality of the wormwood enzyme liquid preparation can be improved.
Drawings
FIG. 1 is a block flow diagram of the process of the present invention;
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example one
Referring to fig. 1, the wormwood enzyme comprises the following components in parts by weight: 0.2 part of wormwood, 0.5 part of brown sugar and 5 parts of water.
Further, brown sugar may be replaced by molasses.
The preparation method of the wormwood enzyme comprises the following steps:
selecting materials: selecting ecologically planted Chinese mugwort, brown sugar decocted by ecologically planted sugarcane and clear water after high-temperature disinfection;
cleaning: soaking fresh folium Artemisiae Argyi in clear water for 20 min, cleaning the surface of the soaked folium Artemisiae Argyi, and removing impurities on the surface of folium Artemisiae Argyi to obtain clean folium Artemisiae Argyi;
fermentation: then mixing clean folium artemisiae argyi with brown sugar and water in proportion, and then putting the mixture into a sealed container for fermentation operation;
and (3) filtering: after fermentation is finished, filtering the fermentation liquor in the container by using a filter screen, wherein the liquid component in the fermentation liquor is wormwood enzyme liquid, containing the wormwood enzyme liquid by using a sprinkling can, and containing the solid component in the fermentation by using another container, wherein the solid component can be used for preparing wormwood enzyme edible vinegar, wormwood enzyme pickled vegetables, wormwood enzyme wine and the like, so that the utilization of resources is improved;
and (3) storing: and (5) storing the spraying pot containing the wormwood enzyme liquid in a room temperature environment.
Furthermore, in the fermentation process, the container needs to be subjected to ultraviolet disinfection treatment, and in the fermentation process, the container is positioned in a dark environment with the temperature of 30-35 ℃ and low oxygen content, and the fermentation time is 6 months.
Specifically, in the container fermentation process, the container is shaken every day in the first month or is opened every day to stir the mixture of the folium artemisiae argyi, the brown sugar and the water.
Particularly, in the storage process, the spraying pot containing the wormwood enzyme liquid is prevented from being irradiated by sunlight.
Example two
Referring to fig. 1, the wormwood enzyme comprises the following components in parts by weight: 2.3 parts of wormwood, 1.3 parts of brown sugar and 15 parts of water.
Further, brown sugar may be replaced by molasses.
The preparation method of the wormwood enzyme comprises the following steps:
selecting materials: selecting ecologically planted Chinese mugwort, brown sugar decocted by ecologically planted sugarcane and clear water after high-temperature disinfection;
cleaning: soaking fresh folium Artemisiae Argyi in clear water for 20 min, cleaning the surface of the soaked folium Artemisiae Argyi, and removing impurities on the surface of folium Artemisiae Argyi to obtain clean folium Artemisiae Argyi;
fermentation: then mixing clean folium artemisiae argyi with brown sugar and water in proportion, and then putting the mixture into a sealed container for fermentation operation;
and (3) filtering: after fermentation, filtering the fermentation liquor in the container by using a filter screen, wherein the liquid component in the fermentation liquor is wormwood enzyme liquid, containing the wormwood enzyme liquid by using a sprinkling can, and containing the solid component in the fermentation by using another container;
and (3) storing: and (5) storing the spraying pot containing the wormwood enzyme liquid in a room temperature environment.
Furthermore, in the fermentation process, the container needs to be subjected to ultraviolet disinfection treatment, and in the fermentation process, the container is positioned in a dark environment with the temperature of 30-35 ℃ and low oxygen content, and the fermentation time is 6 months.
Specifically, in the container fermentation process, the container is shaken every day in the first month or is opened every day to stir the mixture of the folium artemisiae argyi, the brown sugar and the water.
Particularly, in the storage process, the spraying pot containing the wormwood enzyme liquid is prevented from being irradiated by sunlight.
EXAMPLE III
Referring to fig. 1, the wormwood enzyme comprises the following components in parts by weight: 1.5 parts of wormwood, 1 part of brown sugar and 10 parts of water.
Further, brown sugar may be replaced by molasses.
The preparation method of the wormwood enzyme comprises the following steps:
selecting materials: selecting ecologically planted Chinese mugwort, brown sugar decocted by ecologically planted sugarcane and clear water after high-temperature disinfection;
cleaning: soaking fresh folium Artemisiae Argyi in clear water for 20 min, cleaning the surface of the soaked folium Artemisiae Argyi, and removing impurities on the surface of folium Artemisiae Argyi to obtain clean folium Artemisiae Argyi;
fermentation: then mixing clean folium artemisiae argyi with brown sugar and water in proportion, and then putting the mixture into a sealed container for fermentation operation;
and (3) filtering: after fermentation, filtering the fermentation liquor in the container by using a filter screen, wherein the liquid component in the fermentation liquor is wormwood enzyme liquid, containing the wormwood enzyme liquid by using a sprinkling can, and containing the solid component in the fermentation by using another container;
and (3) storing: and (5) storing the spraying pot containing the wormwood enzyme liquid in a room temperature environment.
Furthermore, in the fermentation process, the container needs to be subjected to ultraviolet disinfection treatment, and in the fermentation process, the container is positioned in a dark environment with the temperature of 30-35 ℃ and low oxygen content, and the fermentation time is 6 months.
Specifically, in the container fermentation process, the container is shaken every day in the first month or is opened every day to stir the mixture of the folium artemisiae argyi, the brown sugar and the water.
Particularly, in the storage process, the spraying pot containing the wormwood enzyme liquid is prevented from being irradiated by sunlight.
In examples 1 to 3, three groups of wormwood enzymes (hereinafter referred to as "wormwood leaf volatile oil air freshener") are prepared by controlling the proportion of parts of folium artemisiae argyi, brown sugar and water;
the plate sedimentation method is adopted to observe the indoor antibacterial effect of the folium artemisiae argyi volatile oil air freshener, and the specific method comprises the following steps: uniformly spraying 1mg/mL folium artemisiae argyi volatile oil air freshener, spraying 25mL liquid consumption per 15 square meters in a dormitory, and monitoring the number of microorganisms in the air before and 5 hours after application, wherein the test results are as follows (Table 1):
TABLE 1
As can be seen from table 1, the folium artemisiae argyi enzyme prepared by the ratio of folium artemisiae argyi, brown sugar and water in the third embodiment has the highest efficiency in sterilizing air, and meanwhile, the raw materials for preparing the folium artemisiae argyi enzyme are nontoxic, so that water, food and air can be sterilized, and the application range of the folium artemisiae argyi enzyme is greatly enlarged.
Then, a punching method is adopted to specially study the inhibition effect of several bacterial pathogenic bacteria, and a multiple dilution method is used to determine the Minimum Inhibitory Concentration (MIC) of the air freshener with the folium artemisiae argyi volatile oil, the air freshener with the folium artemisiae argyi volatile oil has the best inhibition effect on staphylococcus aureus, escherichia coli and pseudomonas aeruginosa are used secondly, and typhoid bacillus and klebsiella pneumoniae are used finally, the determination of the minimum inhibitory concentration shows that the minimum inhibitory concentration of the air freshener with the folium artemisiae argyi volatile oil on staphylococcus aureus is 0.2mg/mL, the minimum inhibitory concentrations on escherichia coli and pseudomonas aeruginosa are both 1.6mg/mL, the minimum inhibitory concentrations on typhoid bacillus, klebsiella terrestris and klebsiella pneumoniae are 3.2mg/mL, and the result further proves that the inhibition effect of the folium artemisiae argyi volatile oil on staphylococcus aureus is strongest, see (Table 2)
TABLE 2 bacteriostatic effect of the air freshener containing volatile folium Artemisiae Argyi oil on 6 different bacteria
TABLE 2
Performing in-vitro bacteriostasis test on the folium artemisiae argyi volatile oil by adopting an agar double dilution method, and measuring MIC and MBC of the folium artemisiae argyi volatile oil on several common pathogenic bacteria. The result shows that the effect of the folium artemisiae argyi volatile oil on the bacillus anthracis and the staphylococcus aureus is obvious within the concentration range of 0.0312-2 p mu L/mL, particularly the effect is the best when the MIC of the bacillus anthracis reaches 0.063 mu L/mL, and the result is shown in a table 3.
TABLE 3
The result of an antibacterial test on common pathogenic bacteria of aquatic organisms shows that the folium artemisiae argyi volatile oil has the best antibacterial effect on pseudomonas fluorescens and Aeromonas hydrophila, and the MIC is 0.5 mu L/mL; the antibacterial agent also has certain antibacterial effect on Escherichia coli and parahaemolyticus, and the MIC of the antibacterial agent is 1 muL/mL; the bactericidal effect on Klebsiella oxytoca and Myxococcus coides is slightly weak, and the MIC is 2 muL/mL. The results show that the volatile oil of Artemisia princeps Pampanini can effectively inhibit 6 common pathogenic bacteria of aquatic organisms, and the results are shown in Table 4
TABLE 4 MIC and MBC determination results of folium Artemisiae Argyi volatile oil on 6 common pathogenic bacteria of aquatic organisms/(uL/mL)
TABLE 4
The difference between the air spraying of the folium artemisiae argyi extract and the disinfection of ultraviolet rays in the gynecological ward is as follows:
compared with the moxa extract before disinfection, the number of bacterial colonies in the air of a ward is remarkably reduced (P is less than 0.01 or 0.05) 30 minutes to 12 hours after the high-dose disinfection of the moxa extract, the number of bacterial colonies in the air of the ward is remarkably reduced by less than 0.05 in 30 minutes after the low-dose disinfection, and the sterilization rate of 20.9 percent is still obtained after 12 hours; 30 minutes after ultraviolet disinfection, the bacterial colony number in the air of the ward is obviously reduced (P is less than 0.05), but the sterilizing effect disappears after 12 hours. The effect of the moxa extract on air disinfection of the ward is better than that of ultraviolet disinfection.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (8)
1. Moxa enzyme, its characterized in that: the composition and the parts by weight are as follows: 0.2-2.8 parts of wormwood, 0.5-1.5 parts of brown sugar and 5-18 parts of water.
2. The mugwort ferment of claim 1, wherein: the composition and the parts by weight are as follows: 0.7-2.3 parts of wormwood, 0.7-1.3 parts of brown sugar and 8-15 parts of water.
3. The mugwort ferment of claim 1, wherein: the composition and the parts by weight are as follows: 1-2 parts of wormwood, 1 part of brown sugar and 10 parts of water.
4. The mugwort ferment of claim 1, wherein: the brown sugar can be replaced by molasses.
5. The method for preparing mugwort ferment according to any one of claims 1 to 4, wherein: the method comprises the following steps:
selecting materials: selecting ecologically planted Chinese mugwort, brown sugar decocted by ecologically planted sugarcane and clear water after high-temperature disinfection;
cleaning: soaking fresh folium Artemisiae Argyi in clear water for 20 min, cleaning the surface of the soaked folium Artemisiae Argyi, and removing impurities on the surface of folium Artemisiae Argyi to obtain clean folium Artemisiae Argyi;
fermentation: then mixing clean folium artemisiae argyi with brown sugar and water in proportion, and then putting the mixture into a sealed container for fermentation operation;
and (3) filtering: after the fermentation finishes, utilize the filter screen to filter the zymotic fluid in the container, the liquid composition in the zymotic fluid is chinese mugwort grass ferment liquid, carries out the splendid attire with the watering can, and the solid component in will fermenting is held with another container splendid attire:
and (3) storing: and (5) storing the spraying pot containing the wormwood enzyme liquid in a room temperature environment.
6. The method for preparing mugwort ferment of claim 5, wherein the mugwort ferment comprises: in the fermentation process, the container needs to be subjected to ultraviolet disinfection treatment, and in the fermentation process, the container is located in a dark environment with the temperature of 30-35 ℃ and the oxygen content being low, and the fermentation time is 6 months.
7. The method for preparing mugwort ferment of claim 6, wherein the mugwort ferment comprises: in the fermentation process of the folium artemisiae argyi, the brown sugar and the water in the container, the container is shaken every day in the first month or the container is opened every day to stir the mixture of the folium artemisiae argyi, the brown sugar and the water.
8. The method for preparing mugwort ferment of claim 5, wherein the mugwort ferment comprises: in the storage process, avoid sunshine to shine the watering can that contains chinese mugwort grass ferment liquid.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103977443A (en) * | 2014-06-05 | 2014-08-13 | 长沙艾医生物科技有限公司 | Folium artemisiae argyi extract air freshener for air sterilization and mosquito repelling |
| CN104232374A (en) * | 2014-09-26 | 2014-12-24 | 大庆渤基科技开发有限公司 | Environment-friendly ferment soap and preparation method thereof |
| CN106566683A (en) * | 2016-11-04 | 2017-04-19 | 杨新华 | Cleaning liquid as well as preparation method and application thereof |
| CN107518389A (en) * | 2017-09-14 | 2017-12-29 | 深圳市淳睿贸易有限公司 | A kind of preparation method of ferment and enzyme liquid |
| CN109125371A (en) * | 2018-08-16 | 2019-01-04 | 葫芦岛市犹龙健康产业有限公司 | Argy wormwood ferment scraping liquid |
| CN109156635A (en) * | 2018-09-10 | 2019-01-08 | 遵义名城生物科技有限公司 | A kind of animal and fowl fodder enzyme composition and its method for being used to prepare ferment |
-
2020
- 2020-08-25 CN CN202010864114.6A patent/CN112021556A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103977443A (en) * | 2014-06-05 | 2014-08-13 | 长沙艾医生物科技有限公司 | Folium artemisiae argyi extract air freshener for air sterilization and mosquito repelling |
| CN104232374A (en) * | 2014-09-26 | 2014-12-24 | 大庆渤基科技开发有限公司 | Environment-friendly ferment soap and preparation method thereof |
| CN106566683A (en) * | 2016-11-04 | 2017-04-19 | 杨新华 | Cleaning liquid as well as preparation method and application thereof |
| CN107518389A (en) * | 2017-09-14 | 2017-12-29 | 深圳市淳睿贸易有限公司 | A kind of preparation method of ferment and enzyme liquid |
| CN109125371A (en) * | 2018-08-16 | 2019-01-04 | 葫芦岛市犹龙健康产业有限公司 | Argy wormwood ferment scraping liquid |
| CN109156635A (en) * | 2018-09-10 | 2019-01-08 | 遵义名城生物科技有限公司 | A kind of animal and fowl fodder enzyme composition and its method for being used to prepare ferment |
Non-Patent Citations (1)
| Title |
|---|
| 林发中: "酵素技术的推广应用" * |
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