CN112011599A - PCR primer set for reducing non-specific amplification and application thereof - Google Patents
PCR primer set for reducing non-specific amplification and application thereof Download PDFInfo
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Abstract
The invention relates to a PCR primer group for reducing non-specific amplification and application thereof, wherein the PCR primer group comprises an upstream primer and a downstream primer, and the upstream primer and/or the downstream primer sequentially comprises a 5 'end universal sequence, a complementary sequence and a 3' end universal sequence from a 5 'end to a 3' end; the 5 'terminal universal sequence is reverse complementary to the 3' terminal universal sequence, the complementary sequence is specifically complementary and paired with a target sequence, and the complementary sequence contains two RNA bases which are spaced. The PCR primer group can reduce primer dimer and other non-specific amplification products, and increase the specificity of target region sequence amplification, thereby meeting the effectiveness of site capture.
Description
Technical Field
The invention relates to the technical field of molecular biology, in particular to a PCR primer group for reducing non-specific amplification and application thereof.
Background
The Polymerase Chain Reaction (PCR) is a molecular biology technique for amplifying specific DNA fragments, which can be regarded as special DNA replication in vitro, and the biggest characteristic of PCR is that trace amount of DNA can be greatly increased. The basic principle of PCR technology is similar to the natural replication process of DNA, and its specificity depends on oligonucleotide primers complementary to both ends of the target sequence. PCR consists of three basic reaction steps of denaturation-annealing-extension: (1) denaturation of template DNA: heating the template DNA to 93 ℃ for a certain time, dissociating the double-stranded template DNA or the double-stranded DNA formed by PCR amplification to form a single strand so that the single strand can be combined with the primer to prepare for the next reaction; (2) annealing (annealing) of template DNA to primer: heating and denaturing the template DNA into single strands, cooling to about 55 ℃, and pairing and combining the primers and the complementary sequences of the template DNA single strands; (3) extension of the primer: the DNA template-primer combination is used for synthesizing a new semi-reserved replication chain which is complementary with a template DNA chain by taking dNTP as a reaction raw material and a target sequence as a template according to the base complementary pairing and semi-reserved replication principles under the action of DNA polymerase (such as Taq DNA polymerase) at 72 ℃. Repeating the three processes of denaturation, annealing and extension can obtain more 'semi-reserved replication chains', and the new chains can become templates of the next cycle. PCR has been widely used not only in basic research but also in various fields such as medical diagnosis, forensic science, and agricultural science, but non-specific amplification is still a problem to be solved.
For example, multiple PCR is involved in embryo preimplantation detection, multiple PCR primers need to be designed for a detected region, but nonspecific amplification or capture exists in the PCR process, and particularly for a complex sequence region, the capture effect is poor, and the detection effect is influenced.
Disclosure of Invention
Based on this, it is necessary to provide a PCR primer set that can reduce non-specific amplification.
A PCR primer set for reducing non-specific amplification, the PCR primer set comprising an upstream primer and a downstream primer, the upstream primer and/or the downstream primer comprising, in order from 5 'end to 3' end, a 5 'end universal sequence, a complementary sequence, and a 3' end universal sequence; the 5 'terminal universal sequence is reverse complementary to the 3' terminal universal sequence, the complementary sequence is specifically complementary and paired with a target sequence, and the complementary sequence contains two RNA bases which are spaced.
In the PCR reaction of the PCR primer group, after the complementary sequence is specifically complementary and paired with the target sequence, two RNA bases in the primer group can be identified and cut by RNaseH enzyme, so that the 5 ' end universal sequence and the 3 ' end universal sequence are cut off, and the 3 ' end hydroxyl group of the primer left after cutting off is reserved, thereby enabling the primer to be normally extended to carry out the PCR reaction. When the complementary sequence is not specifically matched with the target sequence in a complementary mode, the primer is complemented into a ring through the 5 'end universal sequence and the 3' end universal sequence, and does not participate in the amplification process. Because RNA base in the primer can be cut by RNaseH enzyme only after the complementary sequence is hybridized with the target sequence which is completely matched, and can be extended normally, primer dimer and other non-specific amplification products are reduced, the specificity of target region sequence amplification is increased, and the effectiveness of site capture can be met.
In one embodiment, the bases at the 5 'end and/or the 3' end of the PCR primer set have a modification that blocks primer extension.
In one embodiment, the modification that blocks primer extension is selected from the group consisting of a phosphate group, a dideoxynucleotide, and an internodal arm.
In one embodiment, the two RNA bases are spaced 18bp to 30bp apart.
In one embodiment, the length of the 5 'terminal universal sequence and the 3' terminal universal sequence is 6bp to 20 bp.
In one embodiment, the 5 'terminal universal sequence is shown as SEQ ID NO. 1, and the 3' terminal universal sequence is shown as SEQ ID NO. 2.
The invention also provides a PCR primer group for reducing non-specific amplification, which comprises 410 pairs of the PCR primer group, wherein complementary sequences in the PCR primer group are selected from SEQ ID NO. 3-SEQ ID NO. 822.
The invention also provides application of the PCR primer group in amplification of a target sequence.
The invention also provides a PCR amplification kit, which comprises the PCR primer group and RNaseH enzyme.
The invention also provides a method for genotyping the embryo before implantation, which comprises the following steps: under the condition of RNaseH enzyme, the PCR primer group is used for respectively amplifying target sequences of an embryo sample, a father sample, a mother sample and a proband sample, then sequencing is carried out, and the genotype of the embryo sample is determined according to the sequencing result.
Drawings
FIG. 1 is a schematic diagram of a primer structure according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of the principle of primer amplification according to an embodiment of the present invention.
Detailed Description
In order that the invention may be more fully understood, a more particular description of the invention will now be rendered by reference to specific embodiments thereof that are illustrated in the appended drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
Interpretation of terms
RNaseH enzyme, Ribonuclase H, Chinese name Ribonuclease H, is an endoribonuclease that specifically hydrolyzes RNA in DNA-RNA hybrid strands. RNase H is unable to hydrolyze the phosphodiester bonds in single-and double-stranded DNA or RNA, i.e., to digest single-and double-stranded DNA or RNA.
Pre-embryo implantation testing (PGT-M) is directed to known Monogenic diseases on specific chromosomes, focusing on genetic diagnosis before embryo implantation. The detection substance is mainly 1 cell in 8-cell stage or 3-5 trophoblast cells in blastocyst stage. The detection uses single cell DNA analysis method to detect monogenic hereditary disease. As the technology develops, PGT-M is more and more widely used for clinical detection.
Thalassemia (Thalassemia), abbreviated as Thalassemia, is Thalassemia, i.e., Thalassemia, is a group of hereditary hemolytic anemia diseases in which globin peptide chain synthesis is partially or completely inhibited by globin gene deletion or point mutation. According to the classification of gene defects, there are clinically mainly α chain globin thalassemia (α -thalassemia) and β chain globin thalassemia (β -thalassemia). The current clinical treatment of the disease requires long-term transfusion and deferral therapy, and the implantation of pathogenic embryos can be effectively avoided through embryo pre-implantation detection, so that the birth of the thalassemia patients is avoided. Currently, the thalassemia is detected relatively rarely before embryo implantation, while the thalassemia is detected more frequently before parturition and in adult mutation. For embryo thalassemia detection, nested PCR or multiplex PCR (STR locus or point mutation position) is mostly adopted to directly amplify embryo biopsy cells before single-cell-free whole genome amplification. However, the number of biopsy cells is small, which affects the amplification efficiency. With the development of single cell whole genome amplification technology, the embryo biopsy sample is amplified by the whole genome and then undergoes nested PCR or multiplex PCR amplification to detect the mutation sites. Meanwhile, STR locus amplification can be carried out, and amplification efficiency is improved. With the development of technology in recent years, some SNP chips and high-throughput sequencing are beginning to be applied in PGT-M detection. Haplotype analysis based on SNP sites includes gene chips and high-throughput sequencing technology. The gene chip adopts known mutation sites for detection, has the problems of few sites and incapability of constructing an effective haplotype due to hybridization background interference, and simultaneously needs to be independently customized and expensive, so that large-scale clinical implementation cannot be realized. The high-throughput sequencing technology can obtain hundreds of G data at one time, so that a plurality of sites can be sequenced, and the requirement of site analysis is met.
The method for genotyping thalassemia is divided into STR linkage analysis method and SNP linkage analysis method, STR (short tandem repeat sequence) is a DNA tandem repeat sequence widely existing in eukaryotic and prokaryotic genomes, the nucleotide sequence of the STR tandem repeat sequence is about 2-7 bp, the repetition frequency is usually 10-30 times, alleles on STR loci can be distinguished by the difference of copy numbers of the repeat sequence in PCR (polymerase chain reaction) amplification regions, the alleles can be identified by fluorescence detection after capillary electrophoresis separation, the repetition frequency can be obtained by certain calculation, and haplotype analysis can be carried out according to the alleles on the STR loci in family analysis.
The SNP linkage analysis method comprises whole genome SNP linkage analysis based on a chip technology and regional SNP linkage analysis based on a capture sequencing technology, wherein the chip technology is that SNP locus probes are planted on a chip and hybridized with family genome and embryo samples, SNP loci in the whole genome range are identified, a parent haplotype is constructed, then a gene region to be detected is analyzed, and whether an embryo inherits pathogenic variation or not is confirmed. SNP linkage analysis based on a capture sequencing technology is to perform capture sequencing on SNP loci in a region near a gene to be detected and perform linkage analysis according to a sequencing result so as to confirm whether an embryo has genetic pathogenic variation. The number of STR sites on the genome is limited, so that few STR sites can be used for linkage analysis, particularly upstream of the thalassemia HBA gene, only few STR sites are available, and in some families, the STR sites have no polymorphism and cannot be used for linkage analysis, and in addition, the risk of misdiagnosis caused by the fact that the number of the STR sites is small and Allele Dropout (ADO) is added is high. PGT-M detection based on the SNP chip technology has high cost, poor site change flexibility, complicated operation and need of a special reading instrument.
The PCR primer set for reducing non-specific amplification according to an embodiment of the present invention includes an upstream primer and a downstream primer, and as shown in FIG. 1, the upstream primer and/or the downstream primer includes a 5 'universal sequence, a complementary sequence and a 3' universal sequence in sequence from a 5 'end to a 3' end. The 5 'end general sequence and the 3' end general sequence are reversely complementary, the complementary sequence is specifically complementary and matched with the target sequence, and the complementary sequence contains two RNA bases at intervals.
The amplification principle of the PCR primer group is shown in figure 2, in the PCR reaction, after a complementary sequence is specifically complementary and paired with a target sequence, two RNA bases in the complementary sequence can be identified and cut by RNaseH enzyme, so that a 5 ' end universal sequence and a 3 ' end universal sequence are cut off, and a 3 ' end hydroxyl group is reserved in a primer left after cutting off, so that the primer can be normally extended to carry out the PCR reaction. When the complementary sequence is not specifically matched with the target sequence in a complementary mode, the primer is complemented into a ring through the 5 'end universal sequence and the 3' end universal sequence, and does not participate in the amplification process. Because RNA base in the primer can be cut by RNaseH enzyme only after the complementary sequence is hybridized with the target sequence which is completely matched, and can be extended normally, primer dimer and other non-specific amplification products are reduced, the specificity of target region sequence amplification is increased, and the effectiveness of site capture can be met.
In one particular example, the bases at the 5 'end and/or the 3' end of the PCR primer set have a modification that blocks primer extension. In this way, the presence of the terminal modification can also block primer extension in the case where the 5 '-terminal universal sequence and the 3' -terminal universal sequence are not cleaved, thereby further preventing the occurrence of non-specific amplification. It is understood that any modification that blocks normal primer extension is possible, and is not limited to C3 modification, phosphorylation modification, and the like.
Alternatively, the modification that blocks primer extension is selected from the group consisting of a phosphate group, a dideoxynucleotide, and an internucleotide (e.g., ethylene glycol, the C9 internucleotide, the C18 internucleotide, the dideoxy internucleotide, the C3 internucleotide, etc.).
In one specific example, the two RNA bases are spaced 18bp to 30bp apart. It is understood that the sequence between two RNA bases is the actual extension primer sequence, and the specific length and sequence can be designed according to different target sequences and PCR reactions, but not limited thereto, and the first or second T nearest to the two ends of the primer can be replaced by U base (RNA base) in general design.
In a specific example, the length of the 5 'end universal sequence and the 3' end universal sequence is 6 bp-20 bp, and when the complementary sequence is not specifically and complementarily matched with the target sequence, the cyclization effect of the primer is better, so that non-specific amplification can be better avoided.
In a specific example, the 5 'universal sequence is shown in SEQ ID NO. 1 and the 3' universal sequence is shown in SEQ ID NO. 2.
The PCR primer group for reducing non-specific amplification of one embodiment of the invention comprises 410 pairs of the PCR primer group, and complementary sequences in the PCR primer group are selected from SEQ ID NO. 3-SEQ ID NO. 822.
The PCR amplification kit of an embodiment of the invention comprises the PCR primer group and RNaseH enzyme. Optionally, the PCR amplification kit further comprises polymerase, buffer, dNTPs, and the like.
The embryo genotyping method before implantation of one embodiment of the invention comprises the following steps: and under the condition of the existence of RNaseH enzyme, respectively amplifying target sequences of the embryo sample, the father sample, the mother sample and the proband sample by using the PCR primer group, then sequencing, and determining the genotype of the embryo sample according to the sequencing result.
It is understood that pre-implantation testing of embryos is for embryo samples at the 8-cell stage or the blastocyst stage that are not implanted into the uterus and are not of a living human or animal body, and therefore, pre-implantation embryo genotyping methods are not diagnostic and therapeutic methods for disease. In addition, the embryo sample may be from an embryo that has died or tested for non-pathogenic genes, and is not a diagnostic or therapeutic method for disease. It is understood that the genotyping method using the above-described PCR primer set can also be applied to plant samples and the like, without being limited thereto.
Alternatively, the sample starting material may be genomic DNA or an amplified DNA product, wherein the genomic DNA sample may be DNA extracted from a blood-derived sample, and the embryo sample may be subjected to single-cell whole genome amplification.
In a specific example, the complementary sequence of the PCR primer set is selected from SEQ ID NO. 3 to SEQ ID NO. 822. The primer is designed for the thalassemia disease causing gene region and SNP sites linked in the upstream and downstream 2M ranges, and it is understood that the specific sequence is not limited thereto, and can be designed according to different target regions.
The present invention is described in further detail below with reference to specific examples.
Example 1 Mediterranean anemia variant family sample assay
Family information: 1 whole blood sample and 1 embryo biopsy sample of alpha-thalassemia paternity (- -SEA/alpha) mother (HbCS) and proband (alpha/-SEA complex CS mutation)
1. Sample preparation
The family sample is extracted by a whole blood extraction Kit (Tiangen whole blood extraction Kit or other whole blood extraction kits), the embryo sample is extracted by a TaKaRa PicoPLEX WGA Kit (product number R30050) or a Qiagen REPLI-G single Cell Kit (product number 150345), the DNA sample is subjected to concentration determination by a dsDNA HS Assay Kit, and the detection is carried out according to the operation steps of a Kit specification.
2. Targeted sequence capture and library construction
The target region of the embodiment is a thalassemia pathogenic gene HBA and HBB gene region and SNP site regions linked in the upstream and downstream 2M ranges, a genome sequence of the target region captured by a primer combination is constructed by a library, and the captured sequence is connected with joints of different platforms so as to be suitable for high-throughput sequencing of various platforms (Ion Torrent, Illumina and BGI).
Designing primers of SNP loci linked in the thalassemia disease-causing gene region and the upstream and downstream 2M ranges: firstly, downloading all SNP locus regions in each 2M range of the upstream and downstream of HBA and HBB genes, screening SNP loci with MAF value more than or equal to 0.2, filtering out sequence regions with high low-quality repetition, and simultaneously adding HBA and HBB whole gene regions for primer design. The designed primer is added with 5 ' universal sequence TGTTGGGTGTGTTTGG and 3 ' universal sequence CCAAACACACCCAACA, and the 3 ' terminal is modified by C3. The primer sequences are detailed in Table 1 below.
TABLE 1 primer sequences
(1) The mixture was prepared as follows and vortexed for 10 seconds.
| Composition (I) | Volume (μ L) |
| Amplification enzyme mixture (containing RNase H enzyme) | 4 |
| 50ng of DNA template | Y |
| Nuclease-free water | 6-Y |
| Total volume | 10 |
(2) And (3) subpackaging the prepared total volume into two PCR tubes, wherein each PCR tube contains 5 mu L of mixed solution, 5 mu L of primer mixed solution 1 is added into the first tube hole, and 5 mu L of primer mixed solution 2 is added into the second tube.
(3) Vortex for 10 seconds, centrifuge the PCR tube instantaneously to recover the sample as much as possible to the bottom of the tube, then place in the PCR instrument, run the following procedure to amplify the target region on the genome.
(4) The two tubes of target amplification product were pooled in a volume of about 20. mu.L.
(5) Add 3. mu.L of ligation enhancing solution, 5. mu.L of sequencing adaptor (adaptor can be replaced for different sequencing platform adaptors and blunt end ligation), and 2. mu.L of DNA ligase. Cover the mixture, vortex, mix and centrifuge instantly.
(6) The PCR tube was placed in a PCR instrument and run at 22 ℃ for 30 minutes and 72 ℃ for 10 minutes.
3. Library purification
Taking out the purified magnetic beads from a refrigerator at 4 ℃, placing at room temperature, fully shaking to mix the magnetic beads uniformly, incubating at room temperature for 30min, and slowly sucking when in use. Preparing fresh 80% ethanol.
(1) Carefully uncap and add 36 μ L (1.2 x sample volume) of nucleic acid purification reagents to each library. The DNA was thoroughly mixed with the suspension of magnetic beads by 5 strokes with a gun and transferred to a 1.5mL centrifuge tube.
(2) The mixture was left at room temperature for 5 minutes.
(3) Placing the centrifuge tube on a magnetic frame, standing for 5 minutes or waiting for the solution to become clear. Carefully aspirate and discard the supernatant without disturbing the beads.
(4) 200 μ L of freshly prepared 80% ethanol was added for 1 min, and the mixture was placed in a magnetic stand and the residual liquid was aspirated. And repeating the steps once.
(5) Add 30. mu.L of DNA eluent for resuspension, 5min at room temperature, place in magnetic frame, transfer supernatant to new tube.
4. Fluorescent quantitative PCR determination of library concentration
(1) The library was diluted 300-fold, i.e. NFH2O: the diluted library was vortexed for 30s and centrifuged for 5s, 299: 1.
(2) Preparing 1 sample reaction mixed solution MIX according to the following dosage, carrying out vortex oscillation on the prepared reaction mixed solution for 10s, centrifuging for 2s, and placing the centrifuged MIX on an ice box for subpackaging. The order of formulation mixing is NFH2O, Kapa sybr fast master mix, primers, and Rox Dye. The Kapa sybr fast master mix and the Rox Dye reagents were stored immediately in a-20 ℃ freezer after use.
| Composition (I) | Volume (μ L) |
| Kapa sybr fast master mix(2×) | 10 |
| Primer A(10μM) | 0.6 |
| Primer P1(10μM) | 0.6 |
| Rox Dye(High) | 0.4 |
| NFH2O | 4.4 |
| Total of | 16 |
(3) Adding 4 mu L of each sample or standard substance, and operating on ice.
(4) And (3) completing subpackaging and sample adding, carrying out vortex mixing, centrifuging, and operating the following procedures: the PCR system is 20 mu L, the PCR cycle number is 40 cycles, the PCR reaction program is 95 ℃, and the reaction time is 5 min; (95 ℃, 30 s; 60 ℃, 45s)40 cycles.
(5) And (3) calculating the concentration: and (3) drawing a standard curve according to a linear formula of CT (gamma-klgX 0+ b) by using the CT value of the detected standard substance, and multiplying the standard curve by the dilution factor to obtain the original library concentration.
5. Sequencing on machine
Diluting the library to 100pM, taking 3.5 mu L of the library for sequencing, specifically operating according to the specification of a sequencing kit, and operating on a computer according to a reagent matched with a sequencing platform.
6. Data analysis
The detection samples of the embodiment are embryo samples, embryo parent samples and proband samples, all the samples are subjected to high-throughput sequencing by adopting the method, the sequencing result is subjected to informatics analysis, the SNP loci of one party heterozygous and one party homozygous for a couple are selected as effective loci, the haplotype of parents is constructed by the effective SNP locus information of the embryo parents and the proband, then the linkage analysis is carried out on the embryo, and the genotype of the embryo is determined.
Specifically, TMAP alignment software was used to align the offline data with hg19(Ion Reference hg 19). And (4) performing quality control on the comparison result, and performing subsequent data analysis only when the sequencing data with the data volume of more than or equal to 2.5M and the average depth of more than or equal to 500 times are met. And (5) carrying out mutation identification on the result by using a TVC tool comparison. And screening effective SNP sites in the family by using a haplotype analysis algorithm to construct a genetic map of a parent sample in the family, and finally judging whether the embryo sample carries pathogenic variation or not according to a genetic rule.
The sequencing result is automatically analyzed through automatic analysis software, the result shows that the embryo carries the disease gene of the mother, and the known disease locus genotype (clinical detection) is consistent with the detection result of the kit. The results of the measurements are shown in the following table.
Note: italics with bold font-SEADeletion region,/represents MISS site.
Comparative example 1
Family information: the same as in example 1.
1. Sample preparation
The sample is subjected to whole genome amplification.
2. Targeted sequence capture and library construction
A primer set identical to the complementary sequence of the primer set in example 1 but not containing the universal sequence and RNA bases was used.
(1) The mixture was prepared as follows and vortexed for 10 seconds.
| Composition (I) | Volume (μ L) |
| Amplification enzyme mixture (without RNaseH enzyme) | 4 |
| 50ng of DNA template | Y |
| Nuclease-free water | 6-Y |
| Total volume | 10 |
(2) And (3) subpackaging the prepared total volume into two PCR tubes, wherein each PCR tube contains 5 mu L of mixed solution, 5 mu L of primer mixed solution 1 is added into the first tube hole, and 5 mu L of primer mixed solution 2 is added into the second tube.
(3) Vortex for 10 seconds, centrifuge the PCR tube instantaneously to recover the sample as much as possible to the bottom of the tube, then place in the PCR instrument, run the following procedure to amplify the target region on the genome.
(4) The two tubes of target amplification product were pooled in a volume of about 20. mu.L.
(5) Add 3. mu.L of ligation enhancing solution, 5. mu.L of sequencing adaptor (adaptor can be replaced for different sequencing platform adaptors and blunt end ligation), and 2. mu.L of DNA ligase. Cover the mixture, vortex, mix and centrifuge instantly.
(6) The PCR tube was placed in a PCR instrument and run at 22 ℃ for 30 minutes and 72 ℃ for 10 minutes.
3. Library purification
The procedure was the same as in example 1.
4. Fluorescent quantitative PCR determination of library concentration
The procedure was the same as in example 1.
5. On-machine sequencing and data analysis
The procedure is as in example 1.
The characterization data and the effect data of example 1 compared to the comparative example are as follows:
example 2
Family information: whole blood samples and 1 embryo biopsy sample from 1 beta-thalassemia father (CD41-42 heterozygous) mother (beta/beta Chinese type) and proband (CD41-42 heterozygous, beta/beta Chinese type).
1. Sample preparation
The sample is subjected to whole genome amplification.
2. Targeted sequence capture and library construction
The procedure was the same as in example 1.
3. Library purification
The procedure was the same as in example 1.
4. Fluorescent quantitative PCR determination of library concentration
The procedure was the same as in example 1.
5. On-machine sequencing and data analysis
The procedure is as in example 1 above. The detection result is shown in the following table, the embryo carries mother pathogenic deletion type, and the detection result of the kit is the same as the known karyotype result.
Comparative example 2
Family information: the same as in example 2.
1. Sample preparation
The sample is subjected to whole genome amplification.
2. Targeted sequence capture and library construction
A primer set identical to the complementary sequence of the primer set in example 1 but not containing the universal sequence and RNA bases was used.
(1) The mixture was prepared as follows and vortexed for 10 seconds.
| Composition (I) | Volume (μ L) |
| Amplification enzyme mixture (without RNaseH enzyme) | 4 |
| 50ng of DNA template | Y |
| Nuclease-free water | 6-Y |
| Total volume | 10 |
(2) And (3) subpackaging the prepared total volume into two PCR tubes, wherein each PCR tube contains 5 mu L of mixed solution, 5 mu L of primer mixed solution 1 is added into the first tube hole, and 5 mu L of primer mixed solution 2 is added into the second tube.
(3) Vortex for 10 seconds, centrifuge the PCR tube instantaneously to recover the sample as much as possible to the bottom of the tube, then place in the PCR instrument, run the following procedure to amplify the target region on the genome.
(4) The two tubes of target amplification product were pooled in a volume of about 20. mu.L.
(5) Add 3. mu.L of ligation enhancing solution, 5. mu.L of sequencing adaptor (adaptor can be replaced for different sequencing platform adaptors and blunt end ligation), and 2. mu.L of DNA ligase. Cover the mixture, vortex, mix and centrifuge instantly.
(6) The PCR tube was placed in a PCR instrument and run at 22 ℃ for 30 minutes and 72 ℃ for 10 minutes.
3. Library purification
The procedure was the same as in example 1.
4. Fluorescent quantitative PCR determination of library concentration
The procedure was the same as in example 1.
5. On-machine sequencing and data analysis
The procedure is as in example 1.
The characterization data and the effect data of example 2 compared to comparative example 2 are as follows:
the technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Sequence listing
<110> Suzhou Beikang medical instruments Co., Ltd
<120> PCR primer set for reducing non-specific amplification and use thereof
<160> 822
<170> SIPOSequenceListing 1.0
<210> 1
<211> 16
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 1
tgttgggtgt gtttgg 16
<210> 2
<211> 16
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 2
ccaaacacac ccaaca 16
<210> 3
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 3
cggguggaac ctcagtaatc cgaaaagccg ggaucga 37
<210> 4
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 4
ttugagcaag aggagcctgc aatgtcccta gcugcc 36
<210> 5
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 5
ctttcucttc atgtgtattt gctgtctctu agccc 35
<210> 6
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 6
tccuccaatc tttcccgtat gcctggutgt 30
<210> 7
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 7
gggguccagg aagacatact tcttgtaccu acagag 36
<210> 8
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 8
ctucaacacc accgagaacc tgtaugg 27
<210> 9
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 9
cagccguagc agcgaccacc ttgtcuctgg ggagc 35
<210> 10
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 10
cagugggccc agcacaggga tcacatcgtu ggg 33
<210> 11
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 11
caagucgcaa aacatgtttt tctttgaaaa gugagaa 37
<210> 12
<211> 40
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 12
gacugagccc tgagcaaaga gttacctttt aagtatuttg 40
<210> 13
<211> 38
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 13
taauagactt cgtaaggcat tttggttgta cacuccgt 38
<210> 14
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 14
ccaugccttc ttgcaaagct agcttcuctt 30
<210> 15
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 15
cacacugagc attgcagcac ttgtagaagg uggtca 36
<210> 16
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 16
tgauccgccc gccttggcct cccacatatt aaguaat 37
<210> 17
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 17
gcatuaaggg cgattctggt gtgggcugaa aag 33
<210> 18
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 18
ctcugctcac tgcaaaccta tgctgcccgg gutca 35
<210> 19
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 19
tgcugcaggc tgtctcgcac ttgucattg 29
<210> 20
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 20
gagugcggca gcgcctcttc ttcgucct 28
<210> 21
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 21
gatuggggct ctgatgaatt gggaaaccau aacgt 35
<210> 22
<211> 38
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 22
accugaaggg aggagggtag cacttacgca ttacutct 38
<210> 23
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 23
ctuccacata agatgatgtg atgaaagttg ggaugaatt 39
<210> 24
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 24
accucagata gactcggcag agtgaucct 29
<210> 25
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 25
gctgucactg tccttgctca ctccaaacca gucgg 35
<210> 26
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 26
agcugcggtc tagtggaaac ttggagutgg 30
<210> 27
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 27
gcagagggaa ugtccagctt tagccagggu ggc 33
<210> 28
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 28
tccucccggg tctgggtgca atgaatctuc ag 32
<210> 29
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 29
gacutccact ccagggacaa agggcccacu atg 33
<210> 30
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 30
gccuggcatg tcatggtaga gccaagctug ca 32
<210> 31
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 31
gaguggggga tggtggagag tcctgtuaac t 31
<210> 32
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 32
gggtutgtag gtggtggaga cacatg 26
<210> 33
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 33
tcaucaccct agacaaggag acagaacaag aggutc 36
<210> 34
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 34
ctgugccaca tttgctagct gagcctuggg 30
<210> 35
<211> 38
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 35
gccugcacat aatgggtgct ctctgaacac aagcaaua 38
<210> 36
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 36
gctugcttat ccctggcagg tcaataaugc t 31
<210> 37
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 37
cgtcugagaa tgggttaggg ttagggtcua gacc 34
<210> 38
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 38
tgcuccccgg gcggtttctt ctgatcctuc ca 32
<210> 39
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 39
ggccucagaa gcgtaggacg gcugcgc 27
<210> 40
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 40
ccttuatcca ggaagtggac gccaggcutg t 31
<210> 41
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 41
gaggaugtgt ggttaggttc ccccuaccc 29
<210> 42
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 42
cctutcatcc aacccttgcc agaautca 28
<210> 43
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 43
ccgugatact gaccatggcc ttuggc 26
<210> 44
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 44
agguggccac aagggaccct catactcagc uccc 34
<210> 45
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 45
tttutttaat gtcaccatca tttggaaggu cactgc 36
<210> 46
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 46
tggucgggtt gtcagggact gagtttccgg utga 34
<210> 47
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 47
ggcugaggca gaagaatgca ctccagccug ggc 33
<210> 48
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 48
tgcuggtgtc tgagtagcca ctgtactc 28
<210> 49
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 49
agaucccttg ttcattcttc tattgggaaa uataag 36
<210> 50
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 50
tggucagggt ggtctcccga ctucag 26
<210> 51
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 51
gguggggaca cacggtacat ttaaacauga gc 32
<210> 636
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 636
gatugccaca agccaagatc acattgctgc acucca 36
<210> 53
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 53
gggguctgct ttcctctatg tttcuttcat 30
<210> 54
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 54
ccugggcaaa gccccaccct cctatucacc 30
<210> 55
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 55
caacaucttt accaggaata cattccattu ccag 34
<210> 56
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 56
aacuagccgg acatgggtgt catatgcctg taguccc 37
<210> 57
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 57
ggautacagg catgggccaa cacgcccagc uaatt 35
<210> 58
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 58
tacucccagc tacctgggaa ctgtaggcag gaaaautgc 39
<210> 59
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 59
gccaagucac agtaattatt atggaaccua cac 33
<210> 60
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 60
ctguggaact ggagttataa caggccacaa cguggc 36
<210> 61
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 61
gccgacuatg ggaaaagggc gccacagaac aaut 34
<210> 62
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 62
aagutacgtg aacatctgaa gttguctt 28
<210> 63
<211> 46
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 63
atcucaaaac aaaacaaaaa cttcggtgct ttaaaggaca ccauca 46
<210> 64
<211> 38
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 64
ttguaagttt cctccattct agatacaatt ccctuatc 38
<210> 65
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 65
tttuccagtg cattatgcaa atggccaaua agc 33
<210> 66
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 66
gagautcccg tttctccaca ccccugct 28
<210> 67
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 67
ggucagggga aatatggagc gautcatg 28
<210> 68
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 68
acuacaggcg cccgcaccat guccag 26
<210> 69
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 69
gaugagcatt ttcactgttg caagtaaccc ucttg 35
<210> 70
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 70
ggcugaggca ggagaatgat gtgaaccugg 30
<210> 71
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 71
ttcugaggaa tacataacct ctgctacaca tttugcaag 39
<210> 72
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 72
accugagtca cagtcctgct ccattcgcau ggt 33
<210> 73
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 73
cacuccaatc tgggcgagac acugagac 28
<210> 74
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 74
cgtugacttg ctctcgttaa aatggttatc utta 34
<210> 75
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 75
aatuaagacc acaagagaaa ccactacaca cucacc 36
<210> 76
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 76
agttucgaga ctagccttgc ccacauggt 29
<210> 77
<211> 41
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 77
gtcucaaaac aaataaacaa aagccagaca gacacaaaug a 41
<210> 78
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 78
tttuaactac tcagatatgc atctgcaagc acuttg 36
<210> 79
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 79
ggcugaggca ggtggatcag gagucagg 28
<210> 80
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 80
atcucgattt gtcgcccagg gtggagugca 30
<210> 81
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 81
gacuctggag aggtgagtgt cagacgggac ugcc 34
<210> 82
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 82
cacugagagg gaacgctatc tgtccucac 29
<210> 83
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 83
acaucggcgg cgccctgtcc aagcugagc 29
<210> 84
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 84
ggugaccagc aggcagtggg acaggagcug g 31
<210> 85
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 85
gcugcttctg ccctacttgt ttuagctg 28
<210> 86
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 86
cagucactct ttttgtttgt gtgctttgcu ttt 33
<210> 87
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 87
aagaauaaga caaagatgga gaaggaagga tgacugctg 39
<210> 88
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 88
atgugccacc atgcgtggct aatttttgca uttt 34
<210> 89
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 89
cttguggggc acaggtttca gaggugcc 28
<210> 90
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 90
ccgucccggg cacgtctcac aacctgugcc 30
<210> 91
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 91
ggcugtgaga ggtgcctggg acggcutg 28
<210> 92
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 92
tccugagggc agcagataag ggctguacc 29
<210> 93
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 93
ggcuggtggt acagccctta tcugctg 27
<210> 94
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 94
aggugaggca ggtgaggagg gtattgaaag g 31
<210> 95
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 95
catgugctcc ctcctggcag agcagtaact tuaccc 36
<210> 96
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 96
cgcccuggcc tccttataga gccuccg 27
<210> 97
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 97
tgtgutactt aaagtaggag agtgtctctc tttccugtc 39
<210> 98
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 98
taaucagtga gactgtggaa tggcgcagag cugaa 35
<210> 99
<211> 38
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 99
gttuacaacc agtatttacc tagcaagtct tccaucag 38
<210> 100
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 100
cgauagtcac tagtgtgctt gagtgaca 28
<210> 101
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 101
ggguggaggg tggagacgtc cuggcc 26
<210> 102
<211> 23
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 102
cctugacgtt ggtcttgtcg gca 23
<210> 103
<211> 23
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 103
gacucagaga gaacccacca ugg 23
<210> 104
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 104
tgguggggaa ggacaggaac atcctgc 27
<210> 105
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 105
ttcuccccgc aggatgttcc tgtcctuccc 30
<210> 106
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 106
aagtutgacc gggtccaccc gaagcutgt 29
<210> 107
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 107
cctuccccac caccaagacc tactucc 27
<210> 108
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 108
accucccgca acccgcgtga tcctctgccc ugagag 36
<210> 109
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 109
cccucttctc tgcacagctc ctaagccacu gcct 34
<210> 110
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 110
agacuttatt caaagaccag gaagggccgg ugca 34
<210> 111
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 111
ccgutaagct ggagcctcgg tagccgutcc 30
<210> 112
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 112
acatutccgg gatagagaga acccag 26
<210> 113
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 113
ggcuctcagc cagcctagga acaugag 27
<210> 114
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 114
tattutttta aagtgtttct gctgaaataa caaugc 36
<210> 115
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 115
agcauaaacc ctggcgcgcu cgcggc 26
<210> 116
<211> 25
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 116
cggutgaggg tggcctgtgg guccg 25
<210> 117
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 117
ccccucactc tgcttctccc cgcaggaugt t 31
<210> 118
<211> 21
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 118
ccctucgacc cagatcgctc c 21
<210> 119
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 119
ggguggaccc ggtcaacttc aaggugagc 29
<210> 120
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 120
tgctucacag aagccaggaa cttgtc 26
<210> 121
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 121
cccucttctc tgcacagctc ctaagccacu gcc 33
<210> 122
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 122
acucagactt tattcaaaga ccacgggggu acg 33
<210> 123
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 123
ccgugctgac ctccaaatac cgtuaagc 28
<210> 124
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 124
agcugcagag aggttctagc catgtgtguc cc 32
<210> 125
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 125
tccugtgtat cccaggtcca ggaauggg 28
<210> 126
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 126
tttucctcag tgtcaccttg agaataccat utct 34
<210> 127
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 127
caaaucataa ggtcacgaga tugagacc 28
<210> 128
<211> 25
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 128
gutggccagg atggaacttt tuttt 25
<210> 129
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 129
gctugagccc aggagttccc aagaauatcc 30
<210> 130
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 130
gcguaagcca cggcacctgg ccaaaattat utat 34
<210> 131
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 131
ccuggtgtgg gttattuggg aggagg 26
<210> 132
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 132
ctgucccccg tgtctgctga ttacagcuga ag 32
<210> 133
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 133
caaaugattc acagtggtga tggctcugag g 31
<210> 134
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 134
tgtucccccc gtgtcgctgt tcccuccg 28
<210> 135
<211> 24
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 135
ataucgacat gggtgatgcc ugca 24
<210> 136
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 136
tcagugggcg cacacgggcg ctgtgguctg 30
<210> 137
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 137
ccaccagcua cactgtgata ccgguggcag aa 32
<210> 138
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 138
acauagccat cctcatccgc tctgtttcug ca 32
<210> 139
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 139
agcucaaggg tggcatgtgt accccugc 28
<210> 140
<211> 21
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 140
gatugcatcc agcctggacg a 21
<210> 141
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 141
gggcutcatt ttgggttttt ggggggtutt t 31
<210> 142
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 142
cttugggagg cagataacct gaggtcagga gutcg 35
<210> 143
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 143
accucaggtt atctgcctcc caaagugctg 30
<210> 144
<211> 20
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 144
gcucctggcc aacaccttuc 20
<210> 145
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 145
aaaaagaacg aacugagagg tgggaaacat aauc 34
<210> 146
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 146
ctguagtccc agctactact aagaaggcug ag 32
<210> 147
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 147
gccucagtgt cctgtgctca acagaucctc 30
<210> 148
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 148
attuaggaaa agctgtcttg tctgctcagc ugct 34
<210> 149
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 149
aacucacctg agtcccaagt taaggcuatg 30
<210> 150
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 150
accucacaga aaccgtgact tactcguggg 30
<210> 151
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 151
ccauctactg gatgccagaa gacaagacgu gac 33
<210> 152
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 152
aagtugtaca gagttgctcc tgtgttcccg ggucat 36
<210> 153
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 153
ggcagcauca gatttattta ttcctacuca ac 32
<210> 154
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 154
agauggcttc acggaggtca gaagagaa 28
<210> 155
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 155
tuacagcacc cgggagacgg gttcagatcu cg 32
<210> 156
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 156
ggtucccatt tcccaggttc tccagagagc gggcaucca 39
<210> 157
<211> 25
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 157
cuccgtgaag ccatcttccc gtugg 25
<210> 158
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 158
cacuccatat gtggtgggca agtcuggt 28
<210> 159
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 159
cagacutgcc caccacatat ggagtgtuag 30
<210> 160
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 160
ctgugcactg ttgcgtccat gctcagagug gc 32
<210> 161
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 161
ggccuggtca ggacacaagt cucagc 26
<210> 162
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 162
cctucggtag ctgcaaccag gagtgagccc utct 34
<210> 163
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 163
ccucagcaga ccctgggaac acagagaagg gcuc 34
<210> 164
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 164
cctuggagcc ttctgattgt tcaggtcacg cuccc 35
<210> 165
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 165
gcuctcggga ggtagatctt gaccgccucg 30
<210> 166
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 166
catuggaagg acctagccat tggagautaa 30
<210> 167
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 167
caugacagtg catgtaaaaa tgtaaactcc augc 34
<210> 168
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 168
gacuacaggc ctgtgccatc acgcccagcu aat 33
<210> 169
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 169
gcuaggatta caggctcatg ccagcacgcc cagcuaa 37
<210> 170
<211> 25
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 170
gcctugcatt ggcctcgggc autcc 25
<210> 171
<211> 25
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 171
gutggtctgg cttgtcgcaa acucc 25
<210> 172
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 172
taaucccagc tactccggag gccaggacag gagaauggc 39
<210> 173
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 173
agucagagcc agtaagacaa agaatcacga guct 34
<210> 174
<211> 38
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 174
gccucccaga gtgctgaaat ggcatgagcc accguacc 38
<210> 175
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 175
ggutacagtg agctgagttc gttacacugc 30
<210> 176
<211> 40
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 176
cactuggaaa tgttactttc ccatttctcc tttaaautac 40
<210> 177
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 177
guaggacaaa attctgagct gtgaaauga 29
<210> 178
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 178
agcugggcat ggtggtacac atttgtattc tcagcuact 39
<210> 179
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 179
cagucccagg ttcacaccat caccuatg 28
<210> 180
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 180
acguatttcc actactttga attgtctccc tucac 35
<210> 181
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 181
aggauccatg aggtcagagg tucaag 26
<210> 182
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 182
tggucactgg ctgacagtcg gaaaagugat 30
<210> 183
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 183
tcucttcttg ggcatttttc ttgatccaaa uttc 34
<210> 184
<211> 41
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 184
gccugggcaa gatgacaaac cctgtctgta cacaaaauac c 41
<210> 185
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 185
cuctcactca gattattttt ctcaccagua tt 32
<210> 186
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 186
gttuggcctg ggatgggaaa acgttacugt t 31
<210> 187
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 187
tcugtctggt ttcgtctcct gaaattcaut t 31
<210> 188
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 188
gttuacgtct gactccttga cttctttggu aca 33
<210> 189
<211> 24
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 189
ggcuaccagc agcaaactag gugg 24
<210> 190
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 190
taautcttgt attcaaccaa cagtgagtgu tgt 33
<210> 191
<211> 24
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 191
ggagutgcag gaaatctgcc tugg 24
<210> 192
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 192
ggcucacaat gcctcctaaa aataagttga gg 32
<210> 193
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 193
ccucccgaag ttgctgggat tacaaatgta ugc 33
<210> 194
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 194
ggcucctctg taactcctgg gttcatguga t 31
<210> 195
<211> 23
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 195
ccugcccttg actcccaagu gac 23
<210> 196
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 196
cgtugaggca tttatgaaaa acacagactg aucta 35
<210> 197
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 197
ccucagcacc taggagaatg cacacattut t 31
<210> 198
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 198
ttaugctaaa tgcaatgact aggacacgaa gguccc 36
<210> 199
<211> 25
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 199
ccuggcccaa ttcagtgatt tguag 25
<210> 200
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 200
accutccagg tgtaaatgat actcctaccu cag 33
<210> 201
<211> 24
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 201
gguccctctc ttcctgtgtc aucc 24
<210> 202
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 202
tccugactcc agcacctcga gagcagaa 28
<210> 203
<211> 23
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 203
ccaugtcctg aggtacaggg utt 23
<210> 204
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 204
cagugaggag actgagatga aggaagca 28
<210> 205
<211> 25
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 205
aguaactcag cccagaaggc ctugg 25
<210> 206
<211> 25
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 206
cucaactccg gcttctcagg guggg 25
<210> 207
<211> 24
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 207
gcugttggga gtgactggat guga 24
<210> 208
<211> 38
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 208
cgauttatag acaactctga cattgtcacc ccacugac 38
<210> 209
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 209
gacucgcctc aaaacaaaag aaaacaaaac utc 33
<210> 210
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 210
cccuggccgc tctcctgtca gtgttccguc ct 32
<210> 211
<211> 22
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 211
ccuctgggtg gaccaaauga cg 22
<210> 212
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 212
cctuaaaggg ggtcagccac cggaactgca uccc 34
<210> 213
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 213
ggaacuccta gactgtggga aagugt 26
<210> 214
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 214
acucggagac caaagggcgg gggagcgaag acutca 36
<210> 215
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 215
ctgucccctg agctgtccca ttaaacaugg 30
<210> 216
<211> 25
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 216
guacaggcag aagctggcgg cucat 25
<210> 217
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 217
ccugggaggc attggctgag aaguacc 27
<210> 218
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 218
tggugctttt gtattcaatc acctgagaag ag 32
<210> 219
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 219
gccgcauaac ctcctgcaca taccuag 27
<210> 220
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 220
atguggggcg cgctgcagta tcgggtac 28
<210> 221
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 221
ccucccatta tattttaagg agaaccutg 29
<210> 222
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 222
agtuccctct ctttcttttg tattgacacc agautta 37
<210> 223
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 223
gcuctgtgca cccgtttctc atcuac 26
<210> 224
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 224
ctgugcacgg cccagactcc agcagtgact ttuccc 36
<210> 225
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 225
ctugacgttc aggacctgtg acttgaaaau ca 32
<210> 226
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 226
tggtuttgtt atcacttctg agaagatgcc a 31
<210> 227
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 227
ggucagcagg caatgatgag gacaccugt 29
<210> 228
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 228
aaaugtagaa accactctta gctgtcaggc ccuaaa 36
<210> 229
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 229
agutggcagg ttactttttg ttgtaguaac 30
<210> 230
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 230
gtgtugtggc ttctcctggt ttctttcauc ct 32
<210> 231
<211> 21
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 231
gcauccacac gtgatcatut g 21
<210> 232
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 232
agcuggggag gaacctctgc tctaggtag 29
<210> 233
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 233
cagutctact tacaccagga aaggcccutt 30
<210> 234
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 234
cggucaccag cactgttcgt atgaaaggcc cugct 35
<210> 235
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 235
gcuggagccc gggagtctgc agtaagauat 30
<210> 236
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 236
tcauggcttc tcatttcctg ttgccttgaa gcuaat 36
<210> 237
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 237
ccuccgggga ggtttgcaag gatcacaugg 30
<210> 238
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 238
gggugcccct cctcctcctg gtataaagua cg 32
<210> 239
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 239
cugccacagc gtcgtgtgta caactuat 28
<210> 240
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 240
ctgtuggtca gcatctgcat tttgtgugct 30
<210> 241
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 241
tgugaaccgc acaacacacc aaaggcccut ta 32
<210> 242
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 242
attuccgctt tgtaagctag gaattggcag acugcc 36
<210> 243
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 243
tgugcccgct accaaacgtc tttcatauag 30
<210> 244
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 244
tgcugagcac taactagtga gtctcattag ugag 34
<210> 245
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 245
caauaccacg gcatgccaat aaugcag 27
<210> 246
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 246
ctgugctact gggtgctgtg ttatcagugt g 31
<210> 247
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 247
gcugaaatga cgaggtgaac cagccagcuc t 31
<210> 248
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 248
gtgugagccg cgtggtggtt tcacatactt gauttg 36
<210> 249
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 249
gacuaaccgg ctctccacag gataugc 27
<210> 250
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 250
gggucactcc tgaaggaaag tgggttcucc a 31
<210> 251
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 251
gcuctcggag gtgagtacag aaagugg 27
<210> 252
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 252
ctcugggtca gggacaggga agggcatauc tg 32
<210> 253
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 253
gcaucgctgt gtgggaaagg ggguag 26
<210> 254
<211> 38
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 254
ctcuaaggtt ctgaggctga atgggctagg gggcutgc 38
<210> 255
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 255
acuaattgtt tgtatttttg ctagagacag ggutt 35
<210> 256
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 256
cgcuccgggc cagccacttt attttcaagg uatt 34
<210> 257
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 257
ccugtccctg aagtcccgag taaugg 26
<210> 258
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 258
cggugggtct tccacgtcag gtttcacaug ga 32
<210> 259
<211> 24
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 259
ggucagacat ggacttgtga cugg 24
<210> 260
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 260
caugtgtttc catgacaaca gtcagccgcg gugtc 35
<210> 261
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 261
aaaugcctta gggtgcacag cccggcut 28
<210> 262
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 262
acatutcttc agagcagggc aagggaggcu gtg 33
<210> 263
<211> 25
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 263
ccucctcctc tgggatggca aatuc 25
<210> 264
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 264
tgcuccaagt ttgggatgga cctgaa 26
<210> 265
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 265
ccgcutgcct tgaggatgta aaagucg 27
<210> 266
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 266
cugtttgagc gagagttcta cagugag 27
<210> 267
<211> 25
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 267
cgcugactgg ctgtaaaagc caucc 25
<210> 268
<211> 40
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 268
taauattcta cattgatttt tcaaactaaa cgagttutta 40
<210> 269
<211> 25
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 269
gcucacaagc ccagggcatg aguct 25
<210> 270
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 270
ttctugtgat gccacacgga gatgcggguc tg 32
<210> 271
<211> 25
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 271
ccucatggga tcctcctgca agugt 25
<210> 272
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 272
aagugcagcc tgcgaaaggc agtaccgugg g 31
<210> 273
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 273
gaugggggcc aggaacatga ggtugc 26
<210> 274
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 274
ggguggtact gcctcctgac ctugga 26
<210> 275
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 275
gguaccagtt gtctgtctct gggtagggga uga 33
<210> 276
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 276
gccucccttt ccagacagag atgcggaa 28
<210> 277
<211> 24
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 277
cautgaagct ctggttttgg cugc 24
<210> 278
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 278
cccucgggac agtggctttc agtgctgtag cugcc 35
<210> 279
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 279
tcucgcaccc actccaccga atcugag 27
<210> 280
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 280
tggucacgcg ggtggtcgag atttccatca tgga 34
<210> 281
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 281
ggugtcagag aatgcaggtg tgagggcaug g 31
<210> 282
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 282
accugccccc ctcatcccag aagatcgagu tga 33
<210> 283
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 283
ccggguggga aacgttgacg gaggcautg 29
<210> 284
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 284
gccugcaccg acgcggagat caccgtgtag guggc 35
<210> 285
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 285
ccugcaggag ccagcgtaga agcacagcau ctg 33
<210> 286
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 286
aggucagagt tggtgtgcca agatctccag guggg 35
<210> 287
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 287
ccucggggaa aactgccttt catctctacc ugtg 34
<210> 288
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 288
cctuggccac cctgagatgg tgaggactug cc 32
<210> 289
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 289
tcugcgtttt tccctccagg tacgtucac 29
<210> 290
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 290
acgugttttt ctcccttttc tacacaaaug gt 32
<210> 291
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 291
gaugtccttg tcccttccaa ggggugg 27
<210> 292
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 292
aggucactgc agggagatcc aggaagagug gg 32
<210> 293
<211> 25
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 293
ccugcctcag cctcctgcag atuca 25
<210> 294
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 294
cagutcattt ccttgtgtaa tgcctacccc utga 34
<210> 295
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 295
gcugcagtga gcagagatat gccattautg 30
<210> 296
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 296
tctugaaaaa gcctgttctt tttgctgcta ttgugaa 37
<210> 297
<211> 24
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 297
ggaugaagat gctgcaggta gugg 24
<210> 298
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 298
accucaggca ccttgcagat aacaccagca uggg 34
<210> 299
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 299
gtugaagggg caggagggat ctautctg 28
<210> 300
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 300
ctcutccgct acatgagctc tgggcctgtg gugg 34
<210> 301
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 301
cgcugcaggt ctgggaaggg tacaaugtc 29
<210> 302
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 302
accuggcttc atcctagcac agcaagacag ccagguctc 39
<210> 303
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 303
ccuggctgtc ttgctgtgct aggauga 27
<210> 304
<211> 25
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 304
ccagcutttg ggagcctcat tuccc 25
<210> 305
<211> 24
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 305
caugaagcct gagcctcaca tugc 24
<210> 306
<211> 41
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 306
aggugaacag ggaagtaagt ggtttgggac aggcaggutt g 41
<210> 307
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 307
ggucttcaca ttatcataac ctcucc 26
<210> 308
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 308
ggcucgcgcc tagcttgggt tcuccg 26
<210> 309
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 309
ggguagctac ctttatggtt attgugg 27
<210> 310
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 310
tccuaaacaa attcctacac actccctctg aa 32
<210> 311
<211> 23
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 311
ccutcagcct ggactggtac utc 23
<210> 312
<211> 41
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 312
gtcucagaaa aaaaaagttt tggtgccaac atccccauag c 41
<210> 313
<211> 23
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 313
ctugtgcgct ctgtgagaag utc 23
<210> 314
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 314
ggguggcagt gatgttcacg atcaccccuc cg 32
<210> 315
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 315
gcuaagctct aggtgaggta ctgcucc 27
<210> 316
<211> 25
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 316
cccutaaaac acctcggctg ccucg 25
<210> 317
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 317
ttguagagac aggtttcagt attttggcca ggcuggt 37
<210> 318
<211> 40
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 318
tcttugtttt caaatgcttt cgtttgtagt aaaaaauacc 40
<210> 319
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 319
gcaugaccat gctccgctaa ttuaaa 26
<210> 320
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 320
gctugagcct gggagttcaa taatatgccu ggg 33
<210> 321
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 321
atugaatctc ccaacccaaa gaaguga 27
<210> 322
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 322
ggguatgggc caagaatgag ccaagaucgt 30
<210> 323
<211> 22
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 323
agugtcctgt gtgtgccacu aa 22
<210> 324
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 324
cttuccagaa tgaaccaatg tacttctttc auata 35
<210> 325
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 325
ggucttttgt ttcattctgg agcguta 27
<210> 326
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 326
gctutgggac gactcagcag acacugcc 28
<210> 327
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 327
gguactatgc caatatggga gctcutt 27
<210> 328
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 328
tggugagtgc ctggaggaag gttctaauct g 31
<210> 329
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 329
cagugagctg tgtttaccac accacugc 28
<210> 330
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 330
ttcucctgcc tcagtctacg caacagcugg g 31
<210> 331
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 331
gcuatttttg tacttcaaac agaaaaacug a 31
<210> 332
<211> 41
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 332
actuaaaagc tacggtgtgt ttatctctaa attgaaautt g 41
<210> 333
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 333
gctuggctaa catggcaaaa cgacguctc 29
<210> 334
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 334
agcutattct tcaatctggt gcatguggc 29
<210> 335
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 335
ggcauctgga cttcaccaca gggccuagg 29
<210> 336
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 336
cccuagggcc tcaggcaggt tacggcagau gag 33
<210> 337
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 337
ccaucccttc ctcagcacaa tgtcutcc 28
<210> 338
<211> 40
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 338
actugacgta agacggacag tgtatagtat ggggacuggt 40
<210> 339
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 339
acugataagc tgcaaccctt aagtaacauc tt 32
<210> 340
<211> 38
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 340
agcuaaccgg tgaatcagtt agagaacaaa ctcauccc 38
<210> 341
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 341
gcuctcctat ctcactgcat tagagcut 28
<210> 342
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 342
tggtuctgtc tcccactaga aagccagcuc cc 32
<210> 343
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 343
ccugcgctca agtgttcttc tttcuca 27
<210> 344
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 344
aacuacggac tttggctaat actaacatat caggcuggg 39
<210> 345
<211> 25
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 345
ggugccacca catctaggta agutt 25
<210> 346
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 346
aagucccaac tgtctctaag cagagacaut ac 32
<210> 347
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 347
ctguggagga gtcactccct tcagaaggag ggugg 35
<210> 348
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 348
attucaaaac ctccagcctc ttcacacucc t 31
<210> 349
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 349
cagguaatga cgccttgttc cagcacaccc ucc 33
<210> 350
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 350
ggcuggagaa gagggacttc tcccaacttu cata 34
<210> 351
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 351
ccugttcagt gttttcattg ttacctgutt g 31
<210> 352
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 352
tcucaaaaaa cccccaaaaa gaccgaccug t 31
<210> 353
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 353
gcutctgtcc tgcctctagt tccautt 27
<210> 354
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 354
ccucttcaaa gagcacttct gcuttc 26
<210> 355
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 355
ggugagaaag cagaagtgct cttugaa 27
<210> 356
<211> 40
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 356
agcugaacac acacgcaggc agaaactgat ttccccuaaa 40
<210> 357
<211> 23
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 357
gccugcctgg aggtatatta uct 23
<210> 358
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 358
agguccggag attgagacat ggtgaaaccc ugtg 34
<210> 359
<211> 24
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 359
gagcugaacg ggcagatcat uacc 24
<210> 360
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 360
agtugatctc ctcctgcttc tgaatcgccu cca 33
<210> 361
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 361
acucggtgtg gatgtgggtt ttcucac 27
<210> 362
<211> 40
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 362
ctcugcgcac ggctgcatag cgctagacac taggccuacc 40
<210> 363
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 363
ccugccctga agagcctcgt tcuccc 26
<210> 364
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 364
cacccucctg gctgaggata gcgguggg 28
<210> 365
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 365
ccugtcttac gtggatacca ggagauccg 29
<210> 366
<211> 38
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 366
tgguccagcc cgcaagtcaa caatggcttt tacauttt 38
<210> 367
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 367
gcuccaaggt gccaacctca gaautc 26
<210> 368
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 368
cctuacagat gtccacggag tcgaagtacc ugca 34
<210> 369
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 369
caggucctgg tgatcttgtc ccagcuag 28
<210> 370
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 370
tccugagatt ctggctcaag cgtccuctg 29
<210> 371
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 371
gccucctctc cacttgaaag cccccgggag cagcucc 37
<210> 372
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 372
gccugcagga cgctcgcatc ttttccccut ac 32
<210> 373
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 373
cccuctgtca gtctacatcc cagcctcctg gga 33
<210> 374
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 374
cccugaccat cccagagtcc acaccagaga guccc 35
<210> 375
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 375
cccauttgtc atccacatgg gugggg 26
<210> 376
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 376
ggcuggagaa gtccactcgt ccaaaggcut gg 32
<210> 377
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 377
ttuccaatat tgcctaggct acacttaaca cuca 34
<210> 378
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 378
cagugccaag cctgcctctg actaactccc cacucct 37
<210> 379
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 379
ttcuctggcc acaccaccaa gtgugag 27
<210> 380
<211> 23
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 380
augctgcagg cagagacagu cag 23
<210> 381
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 381
cautgtgttt cttttccgac gauatc 26
<210> 382
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 382
aaaugcccat ttcctcagtg gtcagaga 28
<210> 383
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 383
ccuccaagct tcgggagagg actuaca 27
<210> 384
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 384
cccutaggga tgagggaaca gaccaggaug cc 32
<210> 385
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 385
acuctgttcc ctcgtcccta agggcgugg 29
<210> 386
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 386
cacucgaggg agggacatag cttggccuga g 31
<210> 387
<211> 25
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 387
gauggggctt tctgtgtcug aggaa 25
<210> 388
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 388
gccuttccaa ggtggctgtt gaguag 26
<210> 389
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 389
tcugagccga gcctctcctc ttcuctt 27
<210> 390
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 390
accugggcca ggagctgctt gacctggaug gg 32
<210> 391
<211> 24
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 391
ggcugccgtc ttcgacacgg uagc 24
<210> 392
<211> 19
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 392
ggaugggcca gcactcatg 19
<210> 393
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 393
cccugcttct ctccacaatc acaggugc 28
<210> 394
<211> 40
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 394
ttcucacccc aggcttccag agtatgcacc cccgggucta 40
<210> 395
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 395
cccutccagg aaggccaaca cggugg 26
<210> 396
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 396
gtguctccag agcatggaga agguaga 27
<210> 397
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 397
ccugtgccct caacttgacg ccaucc 26
<210> 398
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 398
ctcucactac ctcggccaca ggacccacug ga 32
<210> 399
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 399
ccugtccctc ctctcggaca ttaucg 26
<210> 400
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 400
ccauggatct tcaggcagta cagccta 27
<210> 401
<211> 25
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 401
gcugccatgc agagcatgtt ctugg 25
<210> 402
<211> 25
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 402
gcctugctca gaagcagtcc cagta 25
<210> 403
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 403
gcugttcatc gggactctgc tctucac 27
<210> 404
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 404
cagugagggt gtcttactgg ccaggatagg gccucgc 37
<210> 405
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 405
cccuatcctg gccagtaaga cacccucac 29
<210> 406
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 406
catugggcct tgctctggag caaagcugga 30
<210> 407
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 407
cccucagaga ggaaggtacc tgcagccuct gc 32
<210> 408
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 408
gcguggtgcc acccagacac tacacccagc ugac 34
<210> 409
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 409
ggcucaagtt ctgggtggaa cagaaccgac uga 33
<210> 410
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 410
ccagugctgt ctcagacatt cccutgc 27
<210> 411
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 411
tgugccgcaa gctgttcctt ggggagcuca t 31
<210> 412
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 412
ggcuggccac ccccacatct gctcacucc 29
<210> 413
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 413
tgguggcgag cacagcaacc ccaggugtcc 30
<210> 414
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 414
gacugggggg ttcatcctgg caaggtcccu cca 33
<210> 415
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 415
ccuggagagg tcgctttgtg aagaaaccau c 31
<210> 416
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 416
cactuttcgg cctcagtgcc acggcccccu ccc 33
<210> 417
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 417
gaugggccca gaaataccct gactgcucaa 30
<210> 418
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 418
gugaagggag ggatggacag aggggugcc 29
<210> 419
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 419
ccucagaaag cgtttctgat ttatgutctc 30
<210> 420
<211> 25
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 420
ccucttctgc tcagaggtca tugtg 25
<210> 421
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 421
agaugtcatg gtcccggtag aaggcgucat 30
<210> 422
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 422
cctuccctta ggcccatgac accttccccu gag 33
<210> 423
<211> 25
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 423
gagcuctgtg tccattttga ggugg 25
<210> 424
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 424
caauccttcc cgtgctcttc tgaacaugca 30
<210> 425
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 425
ccucgcaggt acttttgcct cttgutct 28
<210> 426
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 426
gcuggacgcc gacacctttc cctgcutac 29
<210> 427
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 427
gaugccagca ggacccactt caccagguca g 31
<210> 428
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 428
tgaugccaca agggaacaaa accttgctga aucca 35
<210> 429
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 429
ttuacttcta ggaagctttg ctttcattat ccauca 36
<210> 430
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 430
aaauagtagc aaaaaccatg acatgcaaag augat 35
<210> 431
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 431
aauacgtcag atgtttgatg tgcttgtaat uta 33
<210> 432
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 432
cttuttctgt gacctcaaac attctccaut ta 32
<210> 433
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 433
agautttgtg agatcccact cactaucaag 30
<210> 434
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 434
tttucactat gctataggag agggttaata gtcaaucat 39
<210> 435
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 435
aauccttttg gaaacctaaa tctaaggttt gutaa 35
<210> 436
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 436
cctuccagac atcaagtcct agattggaca agaguaa 37
<210> 437
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 437
cccucgcttc atgagcccat gtauta 26
<210> 438
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 438
tctuaaggga ttgtgaatgg caagaaagcg gucca 35
<210> 439
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 439
atgauatgtc taaattgcac agacagcctu gt 32
<210> 440
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 440
ccauaaagga gttatgttct cttatgtctg gugata 36
<210> 441
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 441
gaauggaact atctgaggat tctcuac 27
<210> 442
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 442
ttguggaaat agcccatgta tcttctctct ctcuctc 37
<210> 443
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 443
aaucaaatat acctagaact gtgcagctct utc 33
<210> 444
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 444
agacuagtaa aaactctagt ggctaagaag accuggt 37
<210> 445
<211> 22
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 445
gcuccaccct ttttgctctu tt 22
<210> 446
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 446
cagugccagc agagggaggc tgaattctcc cucct 35
<210> 447
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 447
atucagagag cctgacaaat agtagatact ctuca 35
<210> 448
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 448
gccuctcttt cctcatttgc aaattgagac aauaat 36
<210> 449
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 449
tcucttgggt tctaacatta ttcagagacc utt 33
<210> 450
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 450
taaugagttt ggtgtaaagc ccaatcucca 30
<210> 451
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 451
caugggagtg gcagtattgg aagcgcutc 29
<210> 452
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 452
ctgugctggg cattttctgg tttgauacc 29
<210> 453
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 453
ccuagccagg cctgaagacc aguatt 26
<210> 454
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 454
ctcuccttct ctctaaactc aaaccaaatu ata 33
<210> 455
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 455
gcugtttgaa agaataacac aatacutga 29
<210> 456
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 456
atctuctttt ggaacttggg acgctaaaac aacuagc 37
<210> 457
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 457
attugaactt gccttttggt aaggtttaca ucc 33
<210> 458
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 458
aaatugtcag tggtgccaag cattaaaaaa accutag 37
<210> 459
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 459
tauacatacc catttcaggt acccagaata ugc 33
<210> 460
<211> 38
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 460
actuaggcct attgtccaac tcaaattaat gtttugat 38
<210> 461
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 461
aggutgatga tgggcaggta gaagauga 28
<210> 462
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 462
caautatccc attcctactg tctccaccag gaugtc 36
<210> 463
<211> 45
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 463
tcaggctgtt uttttcctag aaggctttat taagctttgg cutca 45
<210> 464
<211> 40
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 464
ataugaaaca tttttctttt ctccctacct gatcccutca 40
<210> 465
<211> 25
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 465
gctagtgcac agcagttcct tcttg 25
<210> 466
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 466
tgtugcatca tggcaccatt ttgaaagcag gguggc 36
<210> 467
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 467
gaacucactc aatcctgcaa gaatgguac 29
<210> 468
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 468
cctucccctc catatctggt tactctagat gutta 35
<210> 469
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 469
aauaatgttg tcaggatggt gttatgcaga utg 33
<210> 470
<211> 40
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 470
aaguacctga aaatcctgat gttctaattg caagtcucat 40
<210> 471
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 471
cacautgtct cacgtatcat cacttaacug a 31
<210> 472
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 472
tcaugcttac tttgtcatat cttgctggta gatucta 37
<210> 473
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 473
atuccactga gcttaggttt ttgaatguat 30
<210> 474
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 474
ttatugagtt tgttcactaa aatctcatgg tgauata 37
<210> 475
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 475
cacutactgg tatcacgacc ctagaaccut c 31
<210> 476
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 476
caguccccaa ggctgctcta gtgctttcag utct 34
<210> 477
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 477
gaaugatgag gtaagggcaa ggcatautt 29
<210> 478
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 478
cctucccaga tgcctgaggc catttaccut tc 32
<210> 479
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 479
tttuggccaa tttatcccat ttgtaatgag tauat 35
<210> 480
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 480
tggucccttc caaatctcat gtcctattca catttuaaa 39
<210> 481
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 481
ggauctacag tgttctttac tcuagt 26
<210> 482
<211> 42
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 482
tgtugaaaaa ccctgtttta aggagagaaa aacaaaacua ca 42
<210> 483
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 483
tcuggactcc tggattggga agtugg 26
<210> 484
<211> 38
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 484
ctcuccctca gtaactccga agctgagata aagcuttt 38
<210> 485
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 485
tatuagggga aacaatgaag tgtgatugt 29
<210> 486
<211> 41
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 486
tgcucagggc tcagttacca caatacatca gatgagcugt a 41
<210> 487
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 487
atucaagaaa catgaagtgt agatgacaga gautc 35
<210> 488
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 488
aaatuctttc tcagatatat tgaggacctt cacagugta 39
<210> 489
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 489
aguatttgtc atgtttctcc actgucaa 28
<210> 490
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 490
aauagatgag ttcaggtttg gattacaatc caagguata 39
<210> 491
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 491
gcuagctctt caggtggcag gtaugt 26
<210> 492
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 492
cacugagaca cattctaggg ataatcctgt cugcc 35
<210> 493
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 493
gcucaaaaaa gcagccaggc tgttautt 28
<210> 494
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 494
tgcuggggat ctgctcctgt ccctagtaac uctg 34
<210> 495
<211> 43
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 495
ctaaatcatc ugttagagta gagaaacaca ttgcaagaau ttc 43
<210> 496
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 496
tgauaacctg aaatatattt tccaggtttt tgcutcc 37
<210> 497
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 497
cttuatccat ctaccattta tgggcattta ggutg 35
<210> 498
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 498
atguaacctt ttggccttcc aaaaaggttc augac 35
<210> 499
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 499
ttuaaatgtg tgtgagatct gtagtaatgg cucc 34
<210> 500
<211> 41
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 500
taauaccctc atgttattgc aaatagcaga atttccautt t 41
<210> 501
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 501
ttucccccaa aagaaggagc tucacct 27
<210> 502
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 502
ttguagaaag ggccaaatca attgtggaaa ggauggc 37
<210> 503
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 503
ctuccttatt cctgtcaatt tgcagtacaa gutg 34
<210> 504
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 504
gttuacaggg ttaaatttcc agtgccacat atttguaat 39
<210> 505
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 505
gtutttttga ctgggtatca ctatgtugc 29
<210> 506
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 506
ctgucaaaca tcagtgagaa atgggatttt tgaaa 35
<210> 507
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 507
aauaggtcct tatattctga gattttatgt ctcuat 36
<210> 508
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 508
atttucagga tcaaagtaaa ctagatcaag tgataga 37
<210> 509
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 509
aaaugctgat gtggaagctg ttugaa 26
<210> 510
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 510
caauggcttc aacttaaagt cacaattgca utag 34
<210> 511
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 511
gacutagtga catgagagtt ttctccatat gguttt 36
<210> 512
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 512
ttatuctctt tgtctcagtt tcctcaattg aaaaautaa 39
<210> 513
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 513
agcautccag ggtgtttacg cttttgtttg tuat 34
<210> 514
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 514
ccttuggttg aagaggaatg cctgaaaaau caa 33
<210> 515
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 515
ccucgcatgc tgggtatctt ctggttugat g 31
<210> 516
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 516
gccugacaaa agggtaggcg gtagauaag 29
<210> 517
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 517
tguggggcct ttgactgcat gaccttguga a 31
<210> 518
<211> 38
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 518
aatuaaagga atcataaaat gcatgatgca tgaauaaa 38
<210> 519
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 519
agauttccaa agaccaagca attaattacc tutt 34
<210> 520
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 520
tgcuggggca gtgttaaagg tgatattagc uttc 34
<210> 521
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 521
ccaugagcta tcactaccaa cctauca 27
<210> 522
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 522
ttgugcagga attcatgtgc acataagttt tcattuctc 39
<210> 523
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 523
aaaaautgat tcgtggtttt caggatguaa gg 32
<210> 524
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 524
acttuttcat tttgcatgca gctgtgagaa auaca 35
<210> 525
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 525
tctucacaca tttatagatc tgcttggttc tgacucc 37
<210> 526
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 526
ttgugctgtt ttccgtcttc ctactcauga a 31
<210> 527
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 527
atguaagcga tcaatgagtt taagaggcut g 31
<210> 528
<211> 40
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 528
tgtuattcag atggatgttg tgagttttga aaaaaauatg 40
<210> 529
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 529
ttugaaacag ccatacctca atcaatguga a 31
<210> 530
<211> 43
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 530
gaauaactac aactgcaaaa agttgttaat tgatgcaaau ata 43
<210> 531
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 531
ttgucatttc ctatgtacag atcctttgtg cugta 35
<210> 532
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 532
tatugaaaaa cattttcagc acttgtgctc taatcugtt 39
<210> 533
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 533
ccaugaactt tttgctttgt tttgtttgtu tgt 33
<210> 534
<211> 41
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 534
atauaatgcc tattatcctt ccttatgatg tccagaaugg t 41
<210> 535
<211> 42
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 535
acauaatgaa gaaaatgagg gaatgaaggg aaggaaaguc ac 42
<210> 536
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 536
tgguagaaga gctttgagca aaccaattga acautct 37
<210> 537
<211> 41
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 537
atauggttaa atatgctctc ccatatgttt acagagauga t 41
<210> 538
<211> 42
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 538
taaugagaac aggagacatt tgtctttctg tgcttagcut ac 42
<210> 539
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 539
ccugccgtgt tttgtgcact atccaccuat g 31
<210> 540
<211> 40
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 540
tcauggccaa aatcagtatt aagttcatag aaccaauttt 40
<210> 541
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 541
acutaatact gattttggcc atgaaaagac agugag 36
<210> 542
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 542
ggauctctct aggatataac tggttcctta autat 35
<210> 543
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 543
ccuatttcat agattaagca tgaagggctu aat 33
<210> 544
<211> 38
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 544
agctuggatc ccaacttcag ggaatattat atgauata 38
<210> 545
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 545
ctcucccctg gatggtagga aacttgucat 30
<210> 546
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 546
tttuagaatt aagtccttta cttctcagtg cauttt 36
<210> 547
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 547
agaaaaugca ctgagaagta aaggacutaa 30
<210> 548
<211> 41
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 548
tccugcccag cactgggctt actgttatat gaaataauat a 41
<210> 549
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 549
atugttttag cagcccatga cguaat 26
<210> 550
<211> 42
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 550
ccaugaagaa attttgagaa gctaaatcac ttgatgcaug gc 42
<210> 551
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 551
aaaautgaca ttcttttaaa ggcataaatg ugta 34
<210> 552
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 552
ccauggactg ttacgagtct acagcacagg ggutgg 36
<210> 553
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 553
cauccgcaca cctggagata ctgtgguata 30
<210> 554
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 554
acctucatct actaaggagc aaaatcctga aatcautac 39
<210> 555
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 555
agugcgaaaa tgggctaata caauaggg 28
<210> 556
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 556
tauaaaaaaa gagccaaacg taagctggaa ccutaa 36
<210> 557
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 557
tcttttaagg utccagctta cgtttggctc tuttt 35
<210> 558
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 558
catuaagaca ttgaataagg aagccactgc tugag 35
<210> 559
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 559
aaaugtggtg attcattctg ttcaaactgg autt 34
<210> 560
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 560
acatuacaca catacctata tacaaccaca caga 34
<210> 561
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 561
ggugagtgct aaagcattga tgtgggaaag gggauac 37
<210> 562
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 562
cttugctcag agtctcctgg gaaagauccc 30
<210> 563
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 563
atcugacttt ggactcaaga tttggcaaua at 32
<210> 564
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 564
catugcccag cagaggcatc aagtaaauac a 31
<210> 565
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 565
ccuacattct ccgagctgtt ttccaccucc 30
<210> 566
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 566
cctuactttg aggtgaaagt atggaccaca ugct 34
<210> 567
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 567
tcucatttct gggaaagcat gaagtgaaag uac 33
<210> 568
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 568
gtaugtacgt tacattgcac aaataaagag accuttg 37
<210> 569
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 569
ttuttcatga gcaaaaactg aaagcacaau tca 33
<210> 570
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 570
ttauctacca cagatatgcc tatcatgtaa gtgutag 37
<210> 571
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 571
gcugcagtaa gccaaaatcg tgtgacugca 30
<210> 572
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 572
agauaggtgt agacgaaaat caagaagaaa aacaautat 39
<210> 573
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 573
atuctgctaa ttggtataag taacagaggc aacaacuaa 39
<210> 574
<211> 38
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 574
atauggggct aaatactatt agcagttcca gacaucca 38
<210> 575
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 575
ccaugcaagc agggttccag tataatagtg gauta 35
<210> 576
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 576
ttgugttaaa actgcctgta gtatccagca cgguaac 37
<210> 577
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 577
ttauggctac ataggattct atggtgtatg ugta 34
<210> 578
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 578
agtucaaaca ttgtggaata cagggtguca a 31
<210> 579
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 579
ccucaccagc atctgttgtt tatttacgut tt 32
<210> 580
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 580
aaaugctttc aatctatgac aaagcattgt agauatc 37
<210> 581
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 581
tttugttacc atagccttgt agtatacctu gaag 34
<210> 582
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 582
cacaaaugta aaaacattac atgttcatgg auagg 35
<210> 583
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 583
gtuttgttgc tggataactg tgaacugag 29
<210> 584
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 584
gatuattttc ctggaatttt ccctgaagaa aaautag 37
<210> 585
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 585
gauaccccaa accattgact ggatctcaua g 31
<210> 586
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 586
tacucgcaga tttggatctt atatcccatc ataugta 37
<210> 587
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 587
taugatggga tataagatcc aaatctgcga guaa 34
<210> 588
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 588
gcguggccct tgtgaagctt gccactgaag augtt 35
<210> 589
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 589
aauactatag cacgcctcag aggauaac 28
<210> 590
<211> 41
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 590
ctctuacttt tgatcatcat caaatctgag ccaagccucc a 41
<210> 591
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 591
ttuctccata actcaagtca tcacatccaa auat 34
<210> 592
<211> 38
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 592
agauaaggga acttcagaga ataacctcat cccaugct 38
<210> 593
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 593
cacauacctc aaaataataa aagctgccta utac 34
<210> 594
<211> 38
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 594
gaaucatatt gcgggtgaag agagatagtt tgacutcc 38
<210> 595
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 595
tatucagact aatcagtgta gacaaatgcu gac 33
<210> 596
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 596
acauatcatt gtgtcagaat tctgacatga cuggg 35
<210> 597
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 597
agattuatgg acagaaaaag gaaactgagg uaac 34
<210> 598
<211> 41
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 598
attucggccc aagggtttct ctgtatattg tgaactauaa c 41
<210> 599
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 599
attugcactt atgccagaaa agtctgacag cautc 35
<210> 600
<211> 41
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 600
ttgugcccca atcaaattga cacatacaac tattcagugt g 41
<210> 601
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 601
taugtatgca taacctattg gttttgtact gugaa 35
<210> 602
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 602
aaaugcaagt gattcctagc tactcctgag gcuaag 36
<210> 603
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 603
gguttttcaa gaacaattga ctagccguaa a 31
<210> 604
<211> 40
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 604
ataucaaaaa tggctattaa tgttgtgcag agttgcugga 40
<210> 605
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 605
ggaugatcaa gaaaatgaga gtcaacaacu tg 32
<210> 606
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 606
tttuagatca tcatggctca tatcaatcag tgggautgt 39
<210> 607
<211> 40
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 607
cauccataag tcactggctt tttctaatag gggcaaautt 40
<210> 608
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 608
aattugagga gtaagtctca cgattccaag uttc 34
<210> 609
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 609
tcutcttctt cttctggaag tctgtcucag ac 32
<210> 610
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 610
cagugaaccc aagagggctg acttaaaaut tg 32
<210> 611
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 611
tguatattga gaataaagtg gccttttgtc cutt 34
<210> 612
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 612
attucctatt aagaaggtgg ctgaagctaa aggcguagt 39
<210> 613
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 613
gtcuccacag ggcaatttga tcagatctga guggagg 37
<210> 614
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 614
gccugctcca gatgttttct atgcacuctt 30
<210> 615
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 615
caugccaatg tcaatgcaaa acccaaauaa aca 33
<210> 616
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 616
atcugcagtt ccgtctctag ctctagatcc aacuctg 37
<210> 617
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 617
taauaccaag tataagatca agggttggtc ucca 34
<210> 618
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 618
catuacacaa tttttatgca tgctggcttu taa 33
<210> 619
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 619
atucatggaa ttaaaaactg gcttcacacu gat 33
<210> 620
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 620
cagucagggt cagctgatgt caaacatcat atttauagg 39
<210> 621
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 621
ggcuttgaat gctatccaac acaactttcu ta 32
<210> 622
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 622
aatucacaaa ctccacctgg aaagaagagt ggcuatt 37
<210> 623
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 623
agutatgtga cagaagtttc taagcagcaa ggugtt 36
<210> 624
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 624
tttuggctat tagaactcgg ctcctcttca utta 34
<210> 625
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 625
aacuttgagc caattaaacc tctgttcttt auaa 34
<210> 626
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 626
agauacaggt ttcatgtcac tttggacatg aguaaag 37
<210> 627
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 627
ctuggttagt tcatgtctgt gtgtggacau aa 32
<210> 628
<211> 44
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 628
atuaagaaac tgcacattaa aacaagaaga taccaaccac uaca 44
<210> 629
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 629
ttuattttgt tctgggtatt ttgtggatac tcucaa 36
<210> 630
<211> 41
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 630
caauaagggg gacaggaagt agatttaaga gtgcagaaaa a 41
<210> 631
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 631
ctutttttct gcactcttaa atctacttcc tguccc 36
<210> 632
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 632
gctuattcag ggcagaaaat aaaagctaau aat 33
<210> 633
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 633
atuagctttt attttctgcc ctgaataagc ugtg 34
<210> 634
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 634
aaguaacaaa ctttggacac accatccttu gaa 33
<210> 634
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 634
ttuggttcac attctatagg ttgcctaatc ugaa 34
<210> 635
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 635
aagtugaata agcatccacc ctcccata 28
<210> 637
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 637
attugctttt tggtgtaaaa tgactcttat gcautaa 37
<210> 638
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 638
ctguccgcta ccgacttatt ctgagtutaa 30
<210> 639
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 639
gaauaacgta gtggattgca aattgcaaug t 31
<210> 640
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 640
tttugggcaa ttgcatggtt ctccatgtga uatg 34
<210> 641
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 641
agugagggcc agcatggaca ggaaguaaaa 30
<210> 642
<211> 40
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 642
tatucaggtg aaattgaaac atttcctttg aaaaaauact 40
<210> 643
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 643
ttuataccag taaagagccg aggttctuga a 31
<210> 644
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 644
tcctugtctg cttatcacca atgaagaaaa agtaautgc 39
<210> 645
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 645
caucatcttt tcctcaattt cttcctaccc tutt 34
<210> 646
<211> 38
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 646
agtugacata gtctgcacat taaggaactg agaautta 38
<210> 647
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 647
ttuccattag attcattaga ggctgtagaa gautag 36
<210> 648
<211> 40
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 648
ttauaaacca ttcaaacttg gtatatgatg actcaautgg 40
<210> 649
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 649
aauagcagtg aatttgagtc tggagaaata aaagggutt 39
<210> 650
<211> 38
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 650
ctttuctcta tgtctttgga acaatgacta ttggucat 38
<210> 651
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 651
aaugtatccc taacagagag caatgaagtu ctt 33
<210> 652
<211> 42
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 652
aaauaacaga aatacctaga ggagggaaat aaaaagccua gg 42
<210> 653
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 653
tauaggaaca caataaatgc ttgttgcaua aa 32
<210> 654
<211> 41
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 654
ctguacgaca aacccatgac atgtgtttag ctatguaaca a 41
<210> 655
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 655
gcauggaaag tttttccatt tgtttctatc agcuctg 37
<210> 656
<211> 43
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 656
catucccata catcaacaat agccaagcct agagccaaau cag 43
<210> 657
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 657
tgugcccagc ttggctcttt ctttttatut t 31
<210> 658
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 658
gccaagccua gaataaagtt gaatttctgg aaucaa 36
<210> 659
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 659
aautctgcca taaatggcag gattttcutc t 31
<210> 660
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 660
cgttuttttg aatttgatat gagaagcaaa ggca 34
<210> 661
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 661
ttucatacta agcccagtcc tuccaaa 27
<210> 662
<211> 41
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 662
cacucagttc tcttgcttag agataccacc tttccccuga a 41
<210> 663
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 663
ggcugttgcc aatgtgcatt agcugtt 27
<210> 664
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 664
ggtucctttg ttccctaagt ccaactacta aacuggg 37
<210> 665
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 665
aauccagatg ctcaaggccc ttcauaat 28
<210> 666
<211> 41
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 666
agcuaggccc ttttgctaat catgttcata cctcttauct t 41
<210> 667
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 667
aaacuaaaac gatcctgaga cttccacacu gat 33
<210> 668
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 668
tcauggcaag aaagtgctcg gtgcctttag ugat 34
<210> 669
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 669
acatcaagcg ucccatagac tcaccctgaa gtuctc 36
<210> 670
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 670
ctctuctgcc tattggtcta ttttcccacc cutag 35
<210> 671
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 671
aguttctatt ggtctcctta aacctgtcut g 31
<210> 672
<211> 22
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 672
ccuagggttg gccaatctac uc 22
<210> 673
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 673
tcucttcctg cgtctccaga ataugcaa 28
<210> 674
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 674
taauaagtaa ctaatgcaca gagcacattg auttg 35
<210> 675
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 675
tgutgttgtt tttcttaatt tgctcatgca ugc 33
<210> 676
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 676
atguccccag ttaacctcct atttgacacc acugat 36
<210> 677
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 677
acuaaagcaa tgaggatcta ggcacacgug ta 32
<210> 678
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 678
ataucaggga tgtgaaacag ggtcttgaaa acugtc 36
<210> 679
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 679
aautgcatat cactgggtct tgatgtacag autat 35
<210> 680
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 680
tcttucatat tgaagatgag gaggtcaaga gaugaa 36
<210> 681
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 681
tcugcccatt gatgaattta atatgaggca caguag 36
<210> 682
<211> 38
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 682
atttucctct tatcttccca tatttcagtc aagautca 38
<210> 683
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 683
ccugctccag tgagcaggtt ggttuaa 27
<210> 684
<211> 41
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 684
catuccacta tattagccta aaacacttct gcaaaaauga a 41
<210> 685
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 685
aaaugtggaa ttagacccag gaatgaagau cc 32
<210> 686
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 686
cccuttctct ttctcttccc tatcccttcu cct 33
<210> 687
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 687
gguaaattct taaggccatg aggacugt 28
<210> 688
<211> 38
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 688
catugcactc tagcttgggc aatagggatg aaacucca 38
<210> 689
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 689
aaguggaaag aatagcatct actcttgtuc a 31
<210> 690
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 690
tgttutctct tccagtgttt tccattttag tcautag 37
<210> 691
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 691
acuaaaatgg aaaacactgg aagagaaaca gutt 34
<210> 692
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 692
aaatugttgg tgttcattaa aggtccttca utta 34
<210> 693
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 693
gauataactg tgaatccaag agtgtgatga auac 34
<210> 694
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 694
tttucctttt tgtttcagct tttcactgtg ucaa 34
<210> 695
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 695
aautagtgta atgccaatgt gggttagaau gga 33
<210> 696
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 696
gtuccttagt atacctccca taccatgtgg cucat 35
<210> 697
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 697
agaugatata tgcacagaac aatgccuaga 30
<210> 698
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 698
gaauggtaat tgacagaagg aaactaggat gtgucca 37
<210> 699
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 699
gaugtgaata aatgcatgac acatgcttgu tg 32
<210> 700
<211> 38
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 700
tttuaacctc ttttatggta gagacattgt cttguaaa 38
<210> 701
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 701
tgutcatgga gcataagaaa ttgtgtagac auata 35
<210> 702
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 702
ctaucccttg cccttctgaa tgtcactcug ta 32
<210> 703
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 703
acuggcctca gaggaaactt cagguca 27
<210> 704
<211> 41
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 704
aaauggggaa cagccacaaa caagaaagaa tcaatacutc c 41
<210> 705
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 705
ggagcuttgc aaacctgaga taaacatggu ct 32
<210> 706
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 706
agagacaagc catttctctt tcctcccggu gag 33
<210> 707
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 707
ttutttctca catttctgaa ggctgacucg 30
<210> 708
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 708
tgatuggtgt tacggaccta atgtttgtgu ctc 33
<210> 709
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 709
cagauaaggt cagttgggag ttggggautc 30
<210> 710
<211> 41
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 710
gguggggact taggtgtaag aaatgaatca gcagaggcuc a 41
<210> 711
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 711
ggucttcact gaaccttttc ccauct 26
<210> 712
<211> 40
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 712
atatuatgag ccacaaaggg tttatattga gggaagugtg 40
<210> 713
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 713
acacutccct caatataaac cctttgtggc ucat 34
<210> 714
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 714
tgcuggtgac cgttttggca atccatutcg 30
<210> 715
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 715
acugcagtca ccatcttctg ccaggaagcc ugcac 35
<210> 716
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 716
gctugaaggg gaacaactac ctgcatttaa tgg 33
<210> 717
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 717
ctucaagcac tagtcactgg ccataautt 29
<210> 718
<211> 40
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 718
atauaaaatg ctgctaatgc ttcattacaa acttataucc 40
<210> 719
<211> 42
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 719
tgucctgcct ccagatagat gttttaaaac aacaaaaaug ag 42
<210> 720
<211> 40
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 720
actuagacaa cggagtattg atctgagcac agcagggugt 40
<210> 721
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 721
caggutgcca aagctgtcaa agaaccuctg 30
<210> 722
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 722
tucacagagg aggacaaggc tactatcaca agccugtgg 39
<210> 723
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 723
gacauaagat gtgcgtgtac atggaaaaca acucta 36
<210> 724
<211> 38
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 724
gcttuctggc attataatct agcaaggtta gagaucat 38
<210> 725
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 725
gtuagacaga agggctcagg cttacuatt 29
<210> 726
<211> 25
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 726
cugaccagga accagcagaa aagtg 25
<210> 727
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 727
gcuctcatga gctcctctaa acctgauta 29
<210> 728
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 728
gcaucagcgt gtcatgtctc agcagcagaa 30
<210> 729
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 729
atuatataag aactccagtg aatctgcctt cuca 34
<210> 730
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 730
tgtugacgca tgcctaaaga ggtgaaatta auccc 35
<210> 731
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 731
agucctgtcc tatagaggtc tttcttccaa ccagauttc 39
<210> 732
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 732
agaucacaca tggttgtctt cagttctttt utta 34
<210> 733
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 733
ccacuccagt caccatcttc tgccaggaag ccugcac 37
<210> 734
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 734
cagugactag tgctgcaaga agaacaacua cc 32
<210> 735
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 735
tccugcctcc agatagatgt tttaaaacga auaacaa 37
<210> 736
<211> 41
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 736
actuagacaa ctgagtattg atctgagcac agcagggugt g 41
<210> 737
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 737
caggutgcca aagctgtcaa agaacctcug ggt 33
<210> 738
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 738
tucacagagg aggacaaggc tactatcaca agccugtgg 39
<210> 739
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 739
aaauaaatca cgcctgtttc atctcagcaa aaguatt 37
<210> 740
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 740
agtugttttc catgcaaata catgtcttat gtcutag 37
<210> 741
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 741
atuctaagac ataagacatg tatttgcaug g 31
<210> 742
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 742
atcuaccaag gtcatggatc gagttcagag aaaaa 35
<210> 743
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 743
agugctgttt ctgacatatt ggccactuaa c 31
<210> 744
<211> 41
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 744
atauaagcct tacacaggat tatgaagtct gaaaggautc c 41
<210> 745
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 745
gcucactgac tgcatggaaa cagatucgg 29
<210> 746
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 746
ggaugggggt gtagtttcca ggtcaatgga utta 34
<210> 747
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 747
acccugtcat ctcagcccaa aatcttaagc uga 33
<210> 748
<211> 40
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 748
cctugaagag gttcttcaca tcccttagaa gttgtauttc 40
<210> 749
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 749
aaaaggtgcu ggagaggatg tggaagaaua gg 32
<210> 750
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 750
ttcuttatcc attatatcgc tgatggccat utgg 34
<210> 751
<211> 38
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 751
attagcaaga utaaccaaga aaagaggaga gaagugca 38
<210> 752
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 752
aatucgaacg tctcctttag gttttctagt ttaugca 37
<210> 753
<211> 41
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 753
aaacacggcu gaaagtaatc atataagaca caaacaaaug g 41
<210> 754
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 754
tttuttttag acttgcttgt ttattgggcu ctt 33
<210> 755
<211> 38
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 755
atcuaagccc tgaaactgta acaattctag aaaauaac 38
<210> 756
<211> 38
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 756
tttuagagcc tggatattat tcttttgtca gatguata 38
<210> 757
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 757
ggauatggag aaaagggaac tctttctuac a 31
<210> 758
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 758
acaugagtgt aatgtgtcca ttggatatac tgauttc 37
<210> 759
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 759
cagutggata gaaggattaa gccataatgu ttg 33
<210> 760
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 760
tttucaccta gtcagtcaac aaatgggagc aaugtg 36
<210> 761
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 761
tcuatctgca acacaaattt ccatttgucc 30
<210> 762
<211> 38
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 762
gaagutttgt ttacaatgca ctccttaaga gagcuaga 38
<210> 763
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 763
ctucgtcttt ttcacttagc agtatggcuc c 31
<210> 764
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 764
aaauggagta gaaattcagt ggcctggaau aat 33
<210> 765
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 765
gaucctaggt ttggaaagaa cgtatttgau cca 33
<210> 766
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 766
accuggtcct tattccttgg actttggcaa ugtc 34
<210> 767
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 767
ttugagagtc ctgcatgatt agttgcucag 30
<210> 768
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 768
cccuccctgt gtccatggat cactggtutt t 31
<210> 769
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 769
agautttgcc actacactcc cattuggg 28
<210> 770
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 770
cattutcagt gactcaaccc ttgactttat aaaaguctt 39
<210> 771
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 771
agutacaaca tggtgaatac aagagaaagc uatta 35
<210> 772
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 772
gagugaaacc ttgcctcacg aaacagaata caaaaa 36
<210> 773
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 773
ctugctatgt tgtccaggat aatatcaaac tcutga 36
<210> 774
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 774
atgtugttgt ctactgtcta gtatccctca aguagt 36
<210> 775
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 775
cccutgttta cctttaagat tagagaaggc utga 34
<210> 776
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 776
atutctgacc cacaactcat gcttug 26
<210> 777
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 777
atucttttct ctgtctcttg aaagagcuct t 31
<210> 778
<211> 40
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 778
tccucccagt ttctccagtt tccctttttc ctaatauatc 40
<210> 779
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 779
aauctatctc tttgtaactt accacagcag auttg 35
<210> 780
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 780
agguaggaac ctgtagatac aaaaagttgg agaaa 35
<210> 781
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 781
cttuagtcca cttatcttga ctatutc 27
<210> 782
<211> 40
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 782
catuactgaa aaggaaccct ggttttatat ccacacuttt 40
<210> 783
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 783
gagutttatg acatcttgat ggaggcaaaa ugt 33
<210> 784
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 784
gaaucaggta tcttgcttgt tttggcctat gacugtt 37
<210> 785
<211> 26
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 785
ggacugtggg cattgtagtg aaugtc 26
<210> 786
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 786
cccuaaaaaa tatcttctcc acctgcaaaa ccuacc 36
<210> 787
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 787
tauctcaaaa catgtcaaat tcagtctttc agautt 36
<210> 788
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 788
attuaccaag ccgtcacttc ttagctacaa guctag 36
<210> 789
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 789
caauacaagt atctatcaac agatgcgtat aucaa 35
<210> 790
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 790
atgutcttcc tacactctgg taacttctaa tcuact 36
<210> 791
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 791
caucattttc tgacatatgt cttgcctuta c 31
<210> 792
<211> 38
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 792
cagugccaaa agaaaggcag tcactgatta tgtatugt 38
<210> 793
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 793
ccugaagtca gcaagggctt tgauagt 27
<210> 794
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 794
atctucaatt tgttgcagca acttggaaau ctc 33
<210> 795
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 795
tauatctccc tcgataaagc tttacatcta cauatg 36
<210> 796
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 796
tgtucgccta ttttctgggc acagaauggc 30
<210> 797
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 797
agaugaagag aatcagtttc aatgaagtgc uttg 34
<210> 798
<211> 39
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 798
catuaaacca aaccagctat cactcttctg tcatauttt 39
<210> 799
<211> 32
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 799
ttuccccttt tgaaatttgt ggatgaaaau cc 32
<210> 800
<211> 38
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 800
atauatgttc agagggaaaa attagaaatg gtgautct 38
<210> 801
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 801
agaaagcacg ugtgataaca tgagatttgc aauatg 36
<210> 802
<211> 31
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 802
gggugaatca cagggagatt agcagtgugg g 31
<210> 803
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 803
gggucctgac ttgatgtgtg atgautt 27
<210> 804
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 804
ttauaaactc ataggccata ttgagagctt cutaa 35
<210> 805
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 805
cccuaaaagc agagttttca ccaaguagta 30
<210> 806
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 806
catugcaccc ggctgtcaga gttcagtttc cuact 35
<210> 807
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 807
ctuctttcag tcttgaatga tgaaaagcug aat 33
<210> 808
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 808
taauacctga gtgagtgatg acataatctg uac 33
<210> 809
<211> 37
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 809
aauccagcac aaatataaag actacaagca cagcugg 37
<210> 810
<211> 33
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 810
ccauggccta tgaccgtttt attgccatcu gca 33
<210> 811
<211> 29
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 811
cauggccaga aagaagtaca tgggcucat 29
<210> 812
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 812
gaaugatata gtcgaagtga taccttgaag guaga 35
<210> 813
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 813
caucactgag tacagaaggt cttaggtagt tggutg 36
<210> 814
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 814
atcuaagtct ttaggtaaga cacaagaacc cugct 35
<210> 815
<211> 35
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 815
gaugaggcaa aggttttaga tttttatccc cuctg 35
<210> 816
<211> 36
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 816
aatuaaaaaa aaacaggagt aggaatacca gauagg 36
<210> 817
<211> 24
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 817
aaugcccaca gtgctaggtg utct 24
<210> 818
<211> 27
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 818
ggatucgaca gtagggaaac cagtgta 27
<210> 819
<211> 30
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 819
cauaggtttg gaaagcatgt tcctcaugtc 30
<210> 820
<211> 38
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 820
tgcucaggga ttgattagtt ctcattgaaa tgacuttc 38
<210> 821
<211> 28
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 821
ccuagtcaga gagcagagat gaagucag 28
<210> 822
<211> 34
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 822
ccaugaagag tgaggccacc aaggagatac uctt 34
Claims (10)
1. A PCR primer set for reducing non-specific amplification, wherein the PCR primer set comprises an upstream primer and a downstream primer, and the upstream primer and/or the downstream primer comprises a 5 'terminal universal sequence, a complementary sequence and a 3' terminal universal sequence in sequence from a 5 'end to a 3' end; the 5 'terminal universal sequence is reverse complementary to the 3' terminal universal sequence, the complementary sequence is specifically complementary and paired with a target sequence, and the complementary sequence contains two RNA bases which are spaced.
2. The PCR primer set according to claim 1, wherein the base at the 5 'end and/or the 3' end of the PCR primer set has a modification that blocks primer extension.
3. The PCR primer set according to claim 2, wherein the modification blocking primer extension is selected from the group consisting of a phosphate group, a dideoxynucleotide and an internodal arm.
4. The PCR primer set as claimed in claim 1, wherein the two RNA bases are spaced 18bp to 30bp apart.
5. The PCR primer set according to any one of claims 1 to 4, wherein the length of the 5 'terminal universal sequence and the 3' terminal universal sequence is 6bp to 20 bp.
6. The PCR primer set according to any one of claims 1 to 4, wherein the 5 'universal sequence is shown as SEQ ID NO. 1, and the 3' universal sequence is shown as SEQ ID NO. 2.
7. A PCR primer set for reducing non-specific amplification, which comprises 410 pairs of PCR primer sets as claimed in any one of claims 1 to 6, wherein complementary sequences in the PCR primer sets are selected from SEQ ID NO. 3 to SEQ ID NO. 822.
8. Use of the PCR primer set according to any one of claims 1 to 6 or the PCR primer set according to claim 7 for amplifying a target sequence.
9. A PCR amplification kit comprising the PCR primer set according to any one of claims 1 to 6 or the PCR primer set according to claim 7, and RNaseH enzyme.
10. A method of pre-implantation embryo genotyping comprising the steps of: amplifying target sequences of an embryo sample, a father sample, a mother sample and a proband sample respectively by using the PCR primer set according to any one of claims 1 to 6 or the PCR primer set according to claim 7 in the presence of RNaseH enzyme, sequencing, and determining the genotype of the embryo sample according to the sequencing result.
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| CN202010929502.8A CN112011599A (en) | 2020-09-07 | 2020-09-07 | PCR primer set for reducing non-specific amplification and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010929502.8A CN112011599A (en) | 2020-09-07 | 2020-09-07 | PCR primer set for reducing non-specific amplification and application thereof |
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| CN112011599A true CN112011599A (en) | 2020-12-01 |
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Citations (5)
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| WO2004007755A2 (en) * | 2002-07-15 | 2004-01-22 | Illumina, Inc. | Multiplex nucleic acid reactions |
| US20140329245A1 (en) * | 2013-01-13 | 2014-11-06 | Unitaq Bio | Methods and Compositions for PCR Using Blocked and Universal Primers |
| CN105925678A (en) * | 2016-05-06 | 2016-09-07 | 艾吉泰康生物科技(北京)有限公司 | Primer group used for amplifying plurality of target DNA sequences in sample and applications of primer group |
| CN107058310A (en) * | 2016-08-12 | 2017-08-18 | 艾吉泰康生物科技(北京)有限公司 | A kind of amplicon library constructing method for improving gene low frequency abrupt climatic change sensitivity |
| CN110088296A (en) * | 2016-12-16 | 2019-08-02 | 姆提普力科姆公司 | Improved multiple and multistep amplified reaction and its reagent |
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2020
- 2020-09-07 CN CN202010929502.8A patent/CN112011599A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004007755A2 (en) * | 2002-07-15 | 2004-01-22 | Illumina, Inc. | Multiplex nucleic acid reactions |
| US20140329245A1 (en) * | 2013-01-13 | 2014-11-06 | Unitaq Bio | Methods and Compositions for PCR Using Blocked and Universal Primers |
| CN105925678A (en) * | 2016-05-06 | 2016-09-07 | 艾吉泰康生物科技(北京)有限公司 | Primer group used for amplifying plurality of target DNA sequences in sample and applications of primer group |
| AU2016102399A4 (en) * | 2016-05-06 | 2019-05-16 | Igenetech Biotech (Beijing) Co., Ltd. | Primer set for amplifying multiple target DNA sequences in sample and use thereof |
| CN107058310A (en) * | 2016-08-12 | 2017-08-18 | 艾吉泰康生物科技(北京)有限公司 | A kind of amplicon library constructing method for improving gene low frequency abrupt climatic change sensitivity |
| CN110088296A (en) * | 2016-12-16 | 2019-08-02 | 姆提普力科姆公司 | Improved multiple and multistep amplified reaction and its reagent |
| US20200024651A1 (en) * | 2016-12-16 | 2020-01-23 | Multiplicom Nv | Modified multiplex and multistep amplification reactions and reagents therefor |
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