CN112011443B - Aquatic animal free sperm separation and collection device - Google Patents
Aquatic animal free sperm separation and collection device Download PDFInfo
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- CN112011443B CN112011443B CN202010939234.8A CN202010939234A CN112011443B CN 112011443 B CN112011443 B CN 112011443B CN 202010939234 A CN202010939234 A CN 202010939234A CN 112011443 B CN112011443 B CN 112011443B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
Abstract
The invention belongs to the technical field of biology, and particularly relates to a separating and collecting device for free sperms of aquatic animals. Comprises a grinding mechanism and a collecting mechanism; the grinding mechanism comprises a vertical grinding cavity and a grinding wing horizontally extending from the lower part of the grinding cavity, and the grinding wing covers the top of the collecting mechanism; the grinding cavity is a hollow cavity, the bottom wall is a closed structure, one half of the grinding cavity is closed, and the other half of the grinding cavity is provided with a plurality of through holes; a sealing mechanism is arranged in the cavity; the center of the collecting mechanism is sunken and matched with the bulge at the bottom of the grinding cavity; the top wall of the concave part is a filter screen. According to the invention, through the advantages of the rubbing method and the grinding method, the water pressure is utilized to directly wash and obtain the free sperms, so that the efficiency is improved, the experiment time is saved, and the success rate of the experiment is improved.
Description
The technical field is as follows:
the invention belongs to the technical field of biology, and particularly relates to a separating and collecting device for free sperms of aquatic animals.
Background art:
in recent years, with the increasing development of fishery resources by human beings and the deterioration of ecological environment of water areas, living space of aquatic animals is squeezed, and the resource amount is sharply reduced. More and more researchers are focusing on the research of aquatic organism seed quality preservation technology. The sperm cryopreservation research can preserve the germplasm information of the stock and the improved variety of the aquatic animals for a long time, effectively preserve the stock and the improved variety and endangered organisms, simultaneously provide material guarantee for cross breeding, germplasm optimization and gynogenesis, get rid of season limitation, reduce the cost of breeding parents and greatly improve the feasibility of breeding.
Sperm cryopreservation studies were performed by first obtaining free sperm. Mature sperm of decapod crustaceans are typically obtained from the vas deferens, the spermatophore or the seminiferous vesicle in vitro. For experiments, one of a scrubbing method, a mechanical grinding method or a trypsin digestion method is adopted to remove the spermatic duct, the sperm pod wall, the seminal vesicle and other substances, and then the free sperms with relatively high concentration can be obtained through a plurality of steps of filtering, washing, centrifuging and the like, so that the operation steps are complicated and the obtaining time is long. Because the longer the aquatic animal sperm is ex vivo, the less viable it is. Too long an acquisition time can significantly affect the scientificity and repeatability of subsequent experiments. Therefore, in order to further obtain the free sperm with good vitality and high concentration, a time-saving and efficient free sperm collecting device needs to be invented, so that the efficiency of scientific researchers is doubled, and the success rate and the repeatability of the experiment are improved.
The invention content is as follows:
the invention aims to solve the technical problem that in the traditional experiment for separating and collecting sperms, firstly, a rubbing method, a grinding method, a trypsin digestion method and the like are used for removing substances such as sperm pod walls and the like, and then, free sperms with relatively high concentration can be obtained through a plurality of steps such as washing, centrifugation and the like; the experiment operation is complicated, the acquisition time is long, the efficiency is low, and a lot of inconvenience is brought to the experimenter.
In order to solve the problems, the invention obtains the free sperms by directly washing by water pressure through the advantages of a rubbing method and a grinding method, thereby improving the efficiency, saving the experiment time and improving the success rate of the experiment.
In order to achieve the purpose, the invention is realized by the following technical scheme that the aquatic animal free sperm separation and collection device comprises a grinding mechanism and a collection mechanism; the grinding mechanism comprises a vertical grinding cavity and a grinding wing horizontally extending from the lower part of the grinding cavity, and the grinding wing covers the top of the collecting mechanism, so that the grinding mechanism has a sealing effect on one hand and has a balance maintaining effect on the other hand; the grinding cavity is a hollow cavity, the bottom wall is a closed structure, one half of the grinding cavity is closed, and the other half of the grinding cavity is provided with a plurality of through holes; a sealing mechanism is arranged in the cavity, a sealing ring at the top of the sealing mechanism is arranged to be clung to the inner wall of the grinding cavity, an adjusting bolt is fixedly arranged on the outer surface of the sealing ring, an annular opening is formed in the grinding cavity at a position corresponding to the sealing ring, and a limiting part is arranged at the opening; the bottom of the sealing mechanism is a semicircular sealing sheet which is connected with the sealing ring through a bracket; the position of semicircle closing plate is adjusted in the slip of accessible adjusting bolt, realizes opening and the closure of cavity diapire, separates to 180 degrees when the adjusting bolt with the locating part, and the semicircle closing plate is with bottom closed position coincidence, and the through-hole is opened, and when the adjusting bolt was contradicted with the locating part, the semicircle closing plate realized that the diapire is totally closed with bottom through-hole position coincidence. The center of the collecting mechanism is sunken and matched with the bulge at the bottom of the grinding cavity; the top wall of the concave part is provided with a filter screen; the side wall of the collecting mechanism is divided into two parts which are detachably connected. At the moment, the basic structure of the device is completed, when sperm needs to be separated, the adjusting bolt is firstly slid to be abutted against the limiting piece, the bottom of the cavity is closed, and water is injected into the cavity; and pouring the mixture into a concave cavity of the collecting mechanism 2, rolling the mixture in the concave cavity while rotating the grinding cavity, separating the adjusting bolt from the limiting part after the rolling is finished, flushing the sperms in the concave cavity into the collecting mechanism under the water flow in the cavity, opening the upper part of the collecting mechanism, and transferring the sperms collected in the lower part.
Furthermore, a plurality of grinding balls are uniformly arranged below the bottom wall of the grinding cavity, so that the rubbing and grinding effects can be better achieved.
Furthermore, a piston is arranged in the grinding cavity. The liquid can be sucked into the cavity by the piston, and after grinding is finished, the liquid in the cavity is pressed out by the piston, so that the impact force is larger, and the sperm recovery rate is higher.
Furthermore, the collecting mechanism also comprises a centrifugal filtering mechanism which comprises a centrifugal plate, the outer edge of the centrifugal plate is arranged on a filter screen ring, the filter screen ring is fixedly connected with the inner wall of the collecting mechanism, the inclined plane of the filter screen ring is provided with a plurality of filter screens to play a role in filtering, and the horizontal plane is contacted with the centrifugal plate to play a role in supporting; the center of the centrifugal plate is connected with the rotating column through a bolt, the top of the bolt column is a semi-circular surface, on one hand, the bolt column plays a role in connecting the centrifugal plate with the rotating column, and on the other hand, liquid flowing down from the filter screen can quickly flow to the periphery of the centrifugal plate from the protrusion through the protrusion of the semi-circular surface, so that the effect of assisting centrifugation is achieved; the rotating column is connected with a cross connecting frame below, and the cross connecting frame is abutted against and slidably connected with the bottom wall of the collecting mechanism. The accessible has the instrument of cross decorative pattern and inserts the cross link in, the cross link of quick rotation to drive the centrifugal plate and rotate, play preliminary centrifugal separation's effect, further with the impurity separation in the mixed liquid.
Furthermore, the upper part and the lower part of the collecting mechanism are connected through threads.
Compared with the prior art, the invention has the beneficial effects that:
(1) meanwhile, the grinding and flushing device is arranged, the rubbing method and the grinding method are combined, substances such as the spermatophore wall and the like are removed, the operation is convenient, and the working efficiency is improved.
(2) The piston structure can be selectively added, the water pressure impact force is increased, and the sperm collecting effect is better.
(3) And a centrifugal filtering mechanism is also arranged, so that impurities can be further separated, and the sperms with higher purity can be obtained.
Drawings
FIG. 1 is a schematic structural view of embodiment 1 of the present invention;
FIG. 2 is a schematic view showing the structure of a polishing chamber in example 1;
FIG. 3 is an enlarged view of a portion of FIG. 1A;
FIG. 4 is a cross-sectional view of FIG. 3B-B';
FIG. 5 is a schematic structural view of embodiment 4 of the present invention;
FIG. 6 is a cross-sectional view of FIG. 5C-C';
FIG. 7 is a schematic view of the bottom wall of the collecting mechanism of embodiment 4.
The grinding mechanism 1, the grinding cavity 11, the grinding wing 12, the closing mechanism 13, the adjusting bolt 14, the limiting piece 15, the grinding ball 16, the semicircular closing piece 17, the through hole 18, the collecting mechanism 2, the filter screen 21, the piston 3, the centrifugal filtering mechanism 4, the centrifugal plate 41, the bolt 42, the filter screen ring 43, the rotary column 44 and the cross connecting frame 45.
The specific implementation mode is as follows:
in order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are a part of the embodiments of the present invention, but not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
as shown in figure 1, the aquatic animal free sperm separation and collection device comprises a grinding mechanism 1 and a collection mechanism 2; the grinding mechanism comprises a vertical grinding cavity 11 and a grinding wing 12 horizontally extending from the lower part of the grinding cavity, and the grinding wing 12 covers the top of the collecting mechanism 2, so that the grinding mechanism has a sealing effect on one hand and has a balance maintaining effect on the other hand; as shown in fig. 4, the grinding chamber is a hollow cavity, the bottom wall is a closed structure, one half of the grinding chamber is closed, and the other half of the grinding chamber is provided with a plurality of through holes 18; as shown in fig. 2 and 4, a closing mechanism 13 is arranged in the cavity, a closing ring at the top of the closing mechanism 13 is arranged to be closely attached to the inner wall of the grinding cavity 11, an adjusting bolt 14 is fixedly arranged on the outer surface of the closing ring, the grinding cavity 11 is annularly opened at a position corresponding to the closing ring, and a limiting member 15 is arranged at the opening; the bottom of the sealing mechanism is provided with a semicircular sealing sheet 17 which is connected with the sealing ring through a bracket; the position of semicircle closing plate 17 is adjusted in the slip of accessible adjusting bolt, realizes opening and closing of cavity diapire, separates to 180 degrees when the adjusting bolt with the locating part, and the semicircle closing plate is when coincident with bottom closed position, and the through-hole is opened, and when the adjusting bolt was contradicted with the locating part, the semicircle closing plate was when coincident with bottom through-hole position, realized that the diapire is totally closed. The center of the collecting mechanism 2 is sunken and matched with the bulge at the bottom of the grinding cavity; the top wall of the concave part is provided with a filter screen 21; the side wall of the collecting mechanism is divided into two parts which are detachably connected. At the moment, the basic structure of the device is completed, when sperm needs to be separated, the adjusting bolt is firstly slid to be abutted against the limiting piece, the bottom of the cavity is closed, and water is injected into the cavity; and pouring the mixture into a concave cavity of the collecting mechanism 2, rolling the mixture in the concave cavity while rotating the grinding cavity, separating the adjusting bolt from the limiting part after the rolling is finished, flushing the sperms in the concave cavity into the collecting mechanism under the water flow in the cavity, opening the upper part of the collecting mechanism, and transferring the sperms collected in the lower part.
Example 2:
as shown in FIG. 3, a plurality of grinding balls 16 are uniformly arranged below the bottom wall of the grinding chamber, so that the rubbing and grinding effects can be better achieved.
The rest is the same as in example 1.
Example 3:
as shown in fig. 5, a piston 3 is provided in the grinding chamber. The liquid can be sucked into the cavity by the piston, and after grinding is finished, the liquid in the cavity is pressed out by the piston, so that the impact force is larger, and the sperm recovery rate is higher.
The rest is the same as in example 1 or 2.
Example 4:
as shown in fig. 5, the collecting mechanism 2 further comprises a centrifugal filtering mechanism 4, which comprises a centrifugal plate 41, wherein the outer edge of the centrifugal plate is arranged on a filter screen ring 43, the filter screen ring is fixedly connected with the inner wall of the collecting mechanism, the inclined plane of the filter screen ring is provided with a plurality of filter screens for filtering, and the horizontal plane is contacted with the centrifugal plate for supporting; the center of the centrifugal plate is connected with a rotating column 44 through a bolt 42, the top of the bolt 42 is a semi-circular surface, on one hand, the bolt plays a role in connecting the centrifugal plate with the rotating column, and on the other hand, through the bulge of the semi-circular surface, liquid flowing down from the filter screen 21 can quickly flow to the periphery of the centrifugal plate from the bulge, so that the function of assisting centrifugation is achieved; the lower part of the rotating column 44 is connected with a cross connecting frame 45 which is butted and slidably connected with the bottom wall of the collecting mechanism 2. The accessible has the instrument of cross decorative pattern and inserts the cross link in, the cross link of quick rotation to drive the centrifugal plate and rotate, play preliminary centrifugal separation's effect, further with the impurity separation in the mixed liquid.
The rest is the same as any of examples 1 to 3.
Example 5:
the upper part and the lower part of the collecting mechanism are connected through threads. Is convenient to disassemble and clean.
The above description is only an exemplary embodiment of the present invention, and is not intended to limit the scope of the present invention. Any equivalent changes and modifications that can be made by one skilled in the art without departing from the spirit and principles of the invention should fall within the protection scope of the invention.
Claims (4)
1. The utility model provides an aquatic livestock free sperm separation collection device which characterized in that: comprises a grinding mechanism and a collecting mechanism; the grinding mechanism comprises a vertical grinding cavity and a grinding wing horizontally extending from the lower part of the grinding cavity, and the grinding wing covers the top of the collecting mechanism; the grinding cavity is a hollow cavity, the bottom wall is a closed structure, one half of the grinding cavity is closed, and the other half of the grinding cavity is provided with a plurality of through holes; a sealing mechanism is arranged in the cavity, a sealing ring at the top of the sealing mechanism is arranged to be clung to the inner wall of the grinding cavity, an adjusting bolt is fixedly arranged on the outer surface of the sealing ring, an annular opening is formed in the grinding cavity at a position corresponding to the sealing ring, and a limiting part is arranged at the opening; the bottom of the sealing mechanism is a semicircular sealing sheet which is connected with the sealing ring through a bracket; the center of the collecting mechanism is sunken and matched with the bulge at the bottom of the grinding cavity; the top wall of the concave part is provided with a filter screen; the side wall of the collecting mechanism is divided into two parts which are detachably connected;
a piston is arranged in the grinding cavity.
2. An aquatic animal free sperm separating and collecting device according to claim 1, wherein: a plurality of grinding balls are uniformly arranged below the bottom wall of the grinding cavity.
3. An aquatic animal free sperm separating and collecting device according to claim 1, wherein: the collecting mechanism also comprises a centrifugal filtering mechanism which comprises a centrifugal plate, the outer edge of the centrifugal plate is arranged on a filter screen ring, the filter screen ring is fixedly connected with the inner wall of the collecting mechanism, the inclined surface of the filter screen ring is provided with a plurality of filter screens, and the horizontal surface of the filter screen ring is contacted with the centrifugal plate; the center of the centrifugal plate is connected with the rotating column through a bolt, and the top of the bolt column is a semi-circular surface; the rotating column is connected with a cross connecting frame below, and the cross connecting frame is abutted against and slidably connected with the bottom wall of the collecting mechanism.
4. An aquatic animal free sperm separating and collecting device according to claim 1 or 3, wherein: the upper part and the lower part of the collecting mechanism are connected through threads.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010939234.8A CN112011443B (en) | 2020-09-09 | 2020-09-09 | Aquatic animal free sperm separation and collection device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010939234.8A CN112011443B (en) | 2020-09-09 | 2020-09-09 | Aquatic animal free sperm separation and collection device |
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| CN112011443A CN112011443A (en) | 2020-12-01 |
| CN112011443B true CN112011443B (en) | 2021-05-18 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN208482541U (en) * | 2018-07-06 | 2019-02-12 | 昆明学院 | A kind of experiment soil grinding device |
| CN110479452A (en) * | 2019-07-29 | 2019-11-22 | 西安医学院 | A kind of hermetic type biology dismembyator |
| CN210171623U (en) * | 2019-05-28 | 2020-03-24 | 严佳 | Lower tooth grinder that can open and shut |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10107729B2 (en) * | 2013-02-15 | 2018-10-23 | Douglas T. Gjerde | Isolation, detection and use of biological cells |
| US10845334B2 (en) * | 2019-02-01 | 2020-11-24 | Applied Research Associates, Inc. | Cell extraction using GEITP |
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- 2020-09-09 CN CN202010939234.8A patent/CN112011443B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN208482541U (en) * | 2018-07-06 | 2019-02-12 | 昆明学院 | A kind of experiment soil grinding device |
| CN210171623U (en) * | 2019-05-28 | 2020-03-24 | 严佳 | Lower tooth grinder that can open and shut |
| CN110479452A (en) * | 2019-07-29 | 2019-11-22 | 西安医学院 | A kind of hermetic type biology dismembyator |
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