CN112010431B - Embedding method immobilized microorganism particle fluidized bed sewage treatment method - Google Patents
Embedding method immobilized microorganism particle fluidized bed sewage treatment method Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention provides an embedding immobilized microorganism particle fluidized bed sewage treatment method, which comprises the following steps: microorganism culture, carrier treatment, embedding, immobilization, activation and sewage treatment. The invention has the beneficial effects that: for NH in sewage 3 The removal effect of-N is good, and the removal rate reaches 97.9 to 99.0 percent; in the embedding and fixing processes, the process method has small influence on the microbial activity, the loss of the microbial activity is small, and the relative activity of the microbes is higher and reaches 69.3 to 74.2 percent; the mechanical strength of the immobilized microbial particles is high, and is improved by 15.3 to 21.4 percent compared with the mechanical strength of the immobilized microbial particles adopting the existing calcium alginate gel particles; the chemical stability of the immobilized microorganism particles is good, and the immobilized microorganism particles are placed in 0.05mol/L phosphate buffer solution and do not change for 170 hours; the immobilized microorganism particles have good dispersibility in sewage, can be fully combined with the sewage, and have good water treatment effect.
Description
Technical Field
The invention relates to the field of sewage treatment, in particular to a sewage treatment method of an embedding immobilized microorganism particle fluidized bed.
Background
With the increasing expansion of urban population and the continuous development of industry and agriculture, water environment pollution accidents occur frequently, and the health and even life of people and livestock are seriously harmed. Many lakes and reservoirs cause eutrophication of water bodies due to the discharge of nitrogen and phosphorus, and seriously threaten the production life and ecological balance of human beings. Ammonia nitrogen is one of the main factors causing water eutrophication. In order to meet the continuously improved requirements of the public on the environmental quality, the nation sets up more and more strict emission standards for nitrogen, and research and development of economic and efficient nitrogen removal treatment technology becomes a key point and a hotspot of research in the field of water pollution control engineering. Although there are many methods for removing ammonia effectively, such as physical methods like reverse osmosis, distillation, soil irrigation; the chemical method comprises an ion exchange method, ammonia stripping, a chemical precipitation method, breakpoint chlorination, electrodialysis, electrochemical treatment and catalytic cracking; the biological method has nitrification and algae cultivation, but the physical method has poor treatment effect, and compared with the chemical method, the biological method has the following advantages: 1) Each chemical product is a highly targeted product, and can be out of work when encountering other chemical substances, and the biological agent has spectrum for removing pollutants; 2) The chemical product can temporarily eliminate certain harmful substances and cover odor, but cannot prevent the generation of the harmful substances; 3) After the chemical product is used, residues are left in the water body, which may cause secondary pollution. The biological preparation contains natural microorganisms without pathogenic bacteria and pathogens, the microorganisms take organic nutrients in the sewage as food under the catalysis of enzyme, and after the sewage is purified, the microorganisms are gradually reduced along with the reduction of the pollutants until the microorganisms are eliminated; 4) The paint is non-toxic and non-corrosive, is convenient to use, does not need to add equipment or engineering basically, and saves capital investment. The method improves the treatment efficiency of the organic pollutants difficult to degrade by adding the functional microbial agent into the wastewater treatment system, and is a common biological strengthening technology at present.
Immobilized microbial technology uses chemical or physical means to localize free cells or enzymes to a defined area, allowing them to remain active and reusable. The method is mainly used for fermentation production at first, is used in the field of water treatment in the later 70 s, and becomes a hot point for research of various national scholars in recent years. The immobilized microorganism technology overcomes the defects that biological cells are too small, are difficult to separate from an aqueous solution and are easy to cause secondary pollution, maintains the advantages of high efficiency, strong stability and capability of purifying and maintaining efficient strains, and has wide application prospect in the field of wastewater treatment. The commonly used microorganism immobilization methods mainly include: embedding method, crosslinking method, and carrier binding method. The principle of the entrapment method is to entrap the microbial cells in the network space of the pores of the water-insoluble gel polymer. Cell interception is achieved by polymerization or ion network formation, or by precipitation, or by changing solvent, temperature, pH. The network of gel polymer prevents leakage of cells while allowing matrix infiltration and product diffusion.
The applicant finds that the existing method for treating sewage by an embedding immobilized microorganism particle fluidized bed has the following defects: (1) In the embedding and fixing processes, the process method has great influence on the microbial activity, and the loss of the microbial activity is great; (2) The immobilized microorganism particles are used for sewage treatment to NH in sewage 3 Non-ideal removal of-N, NH 3 the-N removal rate is poor.
Chinese patent CN106086000A discloses a composite microbial sphere and a preparation method thereof, wherein the composite microbial sphere is prepared by carrying out steps of secondary embedding, crosslinking activation and the like on a composite microbial strain. The patent has the following defects: the compound microbial bacterium ball is used for treating NH in sewage 3 Non-ideal removal of-N, NH 3 The N removal rate is only 73%; in addition, the preparation method has great influence on the activity of the microorganism, and the activity loss of the microorganism is great in the embedding and fixing processes.
Chinese patent CN101381151A discloses an embedding method immobilized microorganism particle fluidized bed sewage treatment method, which treats sewage and microorganism particles through the process steps of filling, treating, separating, regenerating and the like by a specific sewage treatment device. The patent has the following defects: in the regeneration process of the microbial particles, the loss of microbial activity is large, which is not beneficial to the subsequent sewage treatment.
Disclosure of Invention
In order to solve the technical problems in the prior art, the invention provides an embedding immobilized microorganism particle fluidized bed sewage treatment method, which aims to realize the following purposes:
(1) The sewage treatment method of the fluidized bed with the immobilized microorganism particles by the embedding method is provided, and the process method has small influence on the activity of the microorganism and small loss of the activity of the microorganism in the embedding and fixing processes;
(2) Provides a sewage treatment method of an embedding immobilized microorganism particle fluidized bed, wherein the immobilized microorganism particle is used for sewage treatment to NH in sewage 3 The removal effect of-N is good.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
an embedding method immobilized microorganism particle fluidized bed sewage treatment method comprises the following steps: microorganism culture, carrier treatment, embedding, immobilization, activation and sewage treatment.
The microorganism culture comprises the steps of respectively putting 10-15 parts of bacillus subtilis, 3~7 parts of actinomycetes, 10-15 parts of thiobacillus denitrificans, 15-20 parts of pseudomonas stutzeri and 5-10 parts of bacillus coagulans into an aseptic culture medium, adding a culture solution, and stirring at the rotation speed of 30-60RPM for 24-48h.
The culture solution comprises the following raw material components: glucose, potassium dihydrogen phosphate, xanthan gum and tryptone, wherein the ratio of glucose: potassium dihydrogen phosphate: xanthan gum: the weight part ratio of the tryptone is (5 to 10): (3~5): 2 (2~5).
Mixing a predetermined part of xanthan gum and chitosan, heating to 70 ℃, and starting stirring at a stirring speed of 150RPM for 20min; adding rhein and zinc citrate chelate in a predetermined part, and shearing and modifying at the rotating speed of 600 to 800RPM for 3 to 6min to obtain the modified xanthan gum.
The xanthan gum comprises: and (3) chitosan: rhein: the weight part ratio of the citric acid chelated zinc is (30-35): (2~3): (5~7): (2~3).
And treating the carrier, wherein the carrier is calcium alginate gel particles. Adding the calcium alginate gel particles into a saturated sodium carbonate-water solution with the volume of 5 times, heating to 25-35 ℃, and stirring at 60RPM for 20min; continuously heating to 35-45 ℃, and stirring at 100RPM for 20min; continuously heating to 50 ℃, preserving heat and standing for 1h; filtering out the calcium alginate gel particles; washing the calcium alginate gel particles for 3 to 5min by using enough 2 percent acetic acid; and then washing the calcium alginate gel particles with sufficient deionized water for later use.
The calcium alginate gel particles have the particle size of 3-4 mm and the density of 900-1100 kg/m 3 。
The embedding is carried out, under the condition of applying a uniform strong magnetic field, the calcium alginate gel particles are placed into deionized water at the temperature of 30-40 ℃, are subjected to ultrasonic dispersion for 5-10 min, and are cooled naturally; mixing the strains cultured in the microbial culture step, putting the mixture into the calcium alginate gel particle solution, keeping the temperature within the range of 20 to 25 ℃, and keeping the temperature for 20 to 30min.
The concentration of the calcium alginate gel particles is 5~7%.
The strength of the uniform strong magnetic field is 200 to 400mt.
The immobilization is carried out by dripping the mixture of the calcium alginate gel particles and each strain into the mixture under the condition of applying a uniform magnetic field3~5% CaCl 2 Stirring the solution at 5-10RPM for 2-2.5h, and filtering out the calcium alginate gel particles; putting the calcium alginate gel particles into 0.02 to 0.04 percent hexamethylenediamine solution at normal temperature, stirring for 30 to 60min, and filtering out the calcium alginate gel particles; crosslinking for 10 to 15min by 0.1 to 0.3 percent of glutaraldehyde, and filtering out the calcium alginate gel particles; spraying 0.3% chitosan solution on the calcium alginate gel particles; the calcium alginate gel particles were washed 2~3 times with deionized water.
The chitosan solution is prepared by dissolving quantitative chitosan in 1% acetic acid aqueous solution.
The strength of the uniform magnetic field is 400 to 600mt.
And (3) activating, namely putting the calcium alginate gel particles in an environment of 12-15 ℃, applying a uniform strong magnetic field with the strength of 400-600mt, increasing the magnetic field strength by 50mt every 30min until the magnetic field strength is 1000mt, and keeping for 2h to prepare the immobilized microorganism particles.
And in the sewage treatment step, the immobilized microorganism particles are contacted with sewage, aeration treatment is carried out under the stirring state until the quality of the sewage reaches the discharge standard, the immobilized microorganism particles are filtered out, and the treated sewage is discharged.
Compared with the prior art, the invention has the following beneficial effects:
(1) The invention relates to a sewage treatment method of an embedding method immobilized microorganism particle fluidized bed, which is used for treating NH in sewage 3 The removal effect of-N is good, and the removal rate reaches 97.9 to 99.0 percent;
(2) The invention discloses a sewage treatment method of an embedding method immobilized microorganism particle fluidized bed, which is characterized in that after sewage is treated, the detection of various indexes of water quality is shown in the following table:
(3) According to the method for treating sewage by the embedding immobilized microorganism particle fluidized bed, the process method has small influence on the activity of microorganisms in the embedding and fixing processes, the loss of the activity of the microorganisms is small, and the relative activity of the microorganisms is high and reaches 69.3 to 74.2 percent;
(4) According to the method for treating sewage by the embedding immobilized microorganism particle fluidized bed, the mechanical strength of the immobilized microorganism particles is high, and compared with the immobilized microorganism particles adopting the existing calcium alginate gel particles, the mechanical strength is improved by 15.3-21.4%;
(5) The immobilized microorganism particle fluidized bed sewage treatment method by the embedding method has good chemical stability of the immobilized microorganism particles, and the immobilized microorganism particles are placed in 0.05mol/L phosphate buffer solution and are unchanged for 170 h;
(6) The immobilized microorganism particle fluidized bed sewage treatment method by the embedding method has the advantages that the immobilized microorganism particles have good dispersibility in sewage, can be fully combined with the sewage, and have good water treatment effect.
Detailed Description
In order to more clearly understand the technical features, objects, and effects of the present invention, specific embodiments of the present invention will now be described.
Example 1
An embedding method immobilized microorganism particle fluidized bed sewage treatment method comprises the following steps: microorganism culture, carrier treatment, embedding, immobilization, activation and sewage treatment.
The microorganism culture is carried out by respectively putting 10 parts of bacillus subtilis, 3 parts of actinomycetes, 10 parts of thiobacillus denitrificans, 20 parts of pseudomonas stutzeri and 10 parts of bacillus coagulans into a sterile culture medium, adding culture solution, stirring at 30RPM and culturing for 24h.
The culture solution comprises the following raw material components: glucose, potassium dihydrogen phosphate, xanthan gum, tryptone, the glucose: potassium dihydrogen phosphate: xanthan gum: the weight ratio of the tryptone is 5:3:2:5.
mixing a predetermined part of xanthan gum and chitosan, heating to 70 ℃, and starting stirring at a stirring speed of 150RPM for 20min; adding rhein and citric acid chelated zinc in a predetermined part, and shearing and modifying at the rotating speed of 600RPM for 3min to obtain the modified xanthan gum.
The xanthan gum comprises: and (3) chitosan: rhein: the weight ratio of the citric acid chelated zinc is 30:2:5:3.
and treating the carrier, wherein the carrier is calcium alginate gel particles. Adding the calcium alginate gel particles into a saturated sodium carbonate-water solution with the volume 5 times that of the calcium alginate gel particles, heating to 25 ℃, and stirring at 60RPM for 20min; continuously heating to 35 ℃, and stirring at 100RPM for 20min; continuously heating to 50 ℃, preserving heat and standing for 1h; filtering out the calcium alginate gel particles; washing the calcium alginate gel particles for 3min by using enough 2% acetic acid; and then washing the calcium alginate gel particles with sufficient deionized water for later use.
The calcium alginate gel particles have the grain diameter of 3mm and the density of 900kg/m 3 。
The embedding, under the condition of applying uniform magnetic field, putting the calcium alginate gel particles into deionized water at 30 ℃, carrying out ultrasonic dispersion for 5min, and naturally cooling; mixing the strains cultured in the step of culturing the microorganisms, putting the mixture into the calcium alginate gel particle solution, keeping the temperature within 20 ℃, and keeping the temperature for 20min.
The concentration of the calcium alginate gel particles is 5 percent.
The strength of the uniform magnetic field is 200mt.
And (3) dropwise adding CaCl into the mixture of the calcium alginate gel particles and the strains under the condition of applying a uniform magnetic field for immobilization 2 Stirring the solution at 5RPM for 2h, and filtering out the calcium alginate gel particles; putting the calcium alginate gel particles into a normal-temperature 0.02% hexamethylene diamine solution, stirring for 30min, and filtering out the calcium alginate gel particles; crosslinking with 0.1% glutaraldehyde for 10min, and filtering out the calcium alginate gel particles; spraying 0.3% chitosan solution on the calcium alginate gel particles; the calcium alginate gel particles were rinsed 2 times with deionized water.
The chitosan solution is prepared by dissolving quantitative chitosan in 1% acetic acid aqueous solution.
The strength of the uniform magnetic field is 400mt.
And (3) activating, namely putting the calcium alginate gel particles in an environment at 12 to 15 ℃, applying a uniform magnetic field with the strength of 600mt, increasing the magnetic field strength by 50mt every 30min until the magnetic field strength is 1000mt, and keeping for 2h to prepare the immobilized microorganism particles.
And the sewage treatment step of contacting the immobilized microorganism particles with sewage, carrying out aeration treatment in a stirring state until the quality of the sewage reaches a discharge standard, filtering out the immobilized microorganism particles, and discharging the sewage after the treatment.
Example 2
An embedding method immobilized microorganism particle fluidized bed sewage treatment method comprises the following steps: microorganism culture, carrier treatment, embedding, immobilization, activation and sewage treatment.
The microorganism culture comprises the steps of respectively putting 15 parts of bacillus subtilis, 7 parts of actinomycetes, 15 parts of thiobacillus denitrificans, 15 parts of pseudomonas stutzeri and 5 parts of bacillus coagulans into a sterile culture medium, adding culture solution, stirring at the rotating speed of 60RPM, and culturing for 48 hours.
The culture solution comprises the following raw material components: glucose, potassium dihydrogen phosphate, xanthan gum and tryptone, wherein the ratio of glucose: potassium dihydrogen phosphate: xanthan gum: the weight ratio of the tryptone is 10.
Mixing a predetermined part of xanthan gum and chitosan, heating to 70 ℃, and starting stirring at a stirring speed of 150RPM for 20min; adding rhein and citric acid chelated zinc in a predetermined part, and shearing and modifying at the rotating speed of 800RPM for 6min to obtain the modified xanthan gum.
The xanthan gum comprises: and (3) chitosan: rhein: the weight ratio of the citric acid chelated zinc is 35.
And (3) treating the carrier, wherein the carrier is calcium alginate gel particles. Adding the calcium alginate gel particles into a saturated sodium carbonate-water solution with the volume 5 times that of the calcium alginate gel particles, heating to 35 ℃, and stirring at 60RPM for 20min; continuously heating to 45 ℃, and stirring at 100RPM for 20min; continuously heating to 50 ℃, preserving heat and standing for 1h; filtering out the calcium alginate gel particles; washing the calcium alginate gel particles for 5min by using enough 2% acetic acid; and then washing the calcium alginate gel particles with sufficient deionized water for later use.
The calcium alginate gel particles have the particle size of 4mm and the density of 1100kg/m 3 。
The embedding, under the condition of applying uniform magnetic field, putting the calcium alginate gel particles into deionized water at 40 ℃, carrying out ultrasonic dispersion for 10min, and naturally cooling; mixing the strains cultured in the step of culturing the microorganisms, putting the mixture into the calcium alginate gel particle solution, keeping the temperature within the range of 25 ℃, and keeping the temperature for 30min.
The concentration of the calcium alginate gel particles is 7 percent.
The strength of the uniform magnetic field is 400mt.
The immobilization is carried out by dripping 5 percent of CaCl into the mixture of the calcium alginate gel particles and each strain under the condition of applying uniform magnetic field 2 Stirring the solution at 10RPM for 2.5h, and filtering out the calcium alginate gel particles; putting the calcium alginate gel particles into a normal-temperature 0.04% hexamethylene diamine solution, stirring for 60min, and filtering out the calcium alginate gel particles; crosslinking with 0.3% glutaraldehyde for 15min, and filtering to obtain calcium alginate gel particles; spraying 0.3% chitosan solution on the calcium alginate gel particles; the calcium alginate gel particles were washed 3 times with deionized water.
The chitosan solution is prepared by dissolving quantitative chitosan in 1% acetic acid aqueous solution.
The strength of the uniform magnetic field is 600mt.
And (3) activating, namely putting the calcium alginate gel particles in an environment of 12-15 ℃, applying a uniform magnetic field with the strength of 600mt, increasing the magnetic field strength by 50mt every 30min until the magnetic field strength is 1000mt, and keeping for 2h to obtain the immobilized microorganism particles.
And the sewage treatment step of contacting the immobilized microorganism particles with sewage, carrying out aeration treatment in a stirring state until the quality of the sewage reaches a discharge standard, filtering out the immobilized microorganism particles, and discharging the sewage after the treatment.
Example 3
An embedding method immobilized microorganism particle fluidized bed sewage treatment method comprises the following steps: microorganism culture, carrier treatment, embedding, immobilization, activation and sewage treatment.
The microorganism culture comprises the steps of respectively putting 12 parts of bacillus subtilis, 5 parts of actinomycetes, 10 parts of thiobacillus denitrificans, 18 parts of pseudomonas stutzeri and 7 parts of bacillus coagulans into a sterile culture medium, adding culture solution, and stirring at the rotating speed of 50RPM for 36 hours.
The culture solution comprises the following raw material components: glucose, potassium dihydrogen phosphate, xanthan gum and tryptone, wherein the ratio of glucose: potassium dihydrogen phosphate: xanthan gum: the weight ratio of tryptone is 8.
Mixing a predetermined part of xanthan gum and chitosan, heating to 70 ℃, and starting stirring at a stirring speed of 150RPM for 20min; adding rhein and citric acid chelated zinc in a predetermined part, and shearing and modifying at the rotating speed of 700RPM for 5min to obtain the modified xanthan gum.
The xanthan gum comprises: and (3) chitosan: rhein: the weight ratio of the citric acid chelated zinc is 30.
And treating the carrier, wherein the carrier is calcium alginate gel particles. Adding the calcium alginate gel particles into saturated sodium carbonate-water solution with the volume 5 times that of the calcium alginate gel particles, heating to 30 ℃, and stirring at 60RPM for 20min; continuously heating to 40 ℃, and stirring at 100RPM for 20min; continuously heating to 50 ℃, preserving heat and standing for 1h; filtering out the calcium alginate gel particles; washing the calcium alginate gel particles with sufficient 2% acetic acid for 4min; and then washing the calcium alginate gel particles with sufficient deionized water for later use.
The calcium alginate gel particles have the particle size of 4mm and the density of 1000kg/m 3 。
The embedding, under the condition of applying a uniform magnetic field, putting the calcium alginate gel particles into deionized water at 35 ℃, ultrasonically dispersing for 7min, and naturally cooling; mixing the strains cultured in the step of culturing the microorganisms, putting the mixture into the calcium alginate gel particle solution, keeping the temperature within 20 ℃, and keeping the temperature for 25min.
The concentration of the calcium alginate gel particles is 6 percent.
The strength of the uniform magnetic field is 300mt.
The immobilization is carried out by dripping 4 percent of CaCl into the mixture of the calcium alginate gel particles and each strain under the condition of applying uniform magnetic field 2 Stirring the solution at 5RPM for 2h, and filtering out the calcium alginate gel particles; putting the calcium alginate gel particles into a normal-temperature 0.03% hexamethylene diamine solution, stirring for 40min, and filtering out the calcium alginate gel particles; crosslinking with 0.2% glutaraldehyde for 15min, and filtering to obtain calcium alginate gel particles; spraying 0.3% chitosan solution on the calcium alginate gel particles; the calcium alginate gel particles were washed 3 times with deionized water.
The chitosan solution is prepared by dissolving quantitative chitosan in 1% acetic acid aqueous solution.
The strength of the uniform magnetic field is 500mt.
And (3) activating, namely placing the calcium alginate gel particles in an environment at 15 ℃, applying a uniform magnetic field with the intensity of 550mt, increasing the magnetic field intensity of 50mt every 30min until the magnetic field intensity is 1000mt, and keeping for 2h to obtain the immobilized microorganism particles.
And the sewage treatment step of contacting the immobilized microorganism particles with sewage, carrying out aeration treatment in a stirring state until the quality of the sewage reaches a discharge standard, filtering out the immobilized microorganism particles, and discharging the sewage after the treatment.
Example 4
An embedding method immobilized microorganism particle fluidized bed sewage treatment method comprises the following steps: microorganism culture, carrier treatment, embedding, immobilization, activation and sewage treatment.
And (3) carrying out microbial culture, namely respectively putting 12 parts of bacillus subtilis, 5 parts of actinomycetes, 12 parts of thiobacillus denitrificans, 17 parts of pseudomonas stutzeri and 8 parts of bacillus coagulans into a sterile culture medium, adding a culture solution, and stirring at a rotating speed of 45RPM for 36 hours.
The culture solution comprises the following raw material components: glucose, potassium dihydrogen phosphate, xanthan gum and tryptone, wherein the ratio of glucose: potassium dihydrogen phosphate: xanthan gum: the weight ratio of tryptone is 7.
Mixing a predetermined part of xanthan gum and chitosan, heating to 70 ℃, and starting stirring at a stirring speed of 150RPM for 20min; adding rhein and citric acid chelated zinc in a predetermined part, and shearing and modifying at 700RPM for 4min to obtain modified xanthan gum.
The xanthan gum comprises: and (3) chitosan: rhein: the weight ratio of the citric acid chelated zinc is 33.
And treating the carrier, wherein the carrier is calcium alginate gel particles. Adding the calcium alginate gel particles into saturated sodium carbonate-water solution with the volume 5 times that of the calcium alginate gel particles, heating to 30 ℃, and stirring at 60RPM for 20min; continuously heating to 40 ℃, and stirring at 100RPM for 20min; continuously heating to 50 ℃, preserving heat and standing for 1h; filtering out the calcium alginate gel particles; washing the calcium alginate gel particles with sufficient 2% acetic acid for 4min; and then washing the calcium alginate gel particles with sufficient deionized water for later use.
The calcium alginate gel particles have the particle size of 3.5mm and the density of 1050kg/m 3 。
The embedding, under the condition of applying a uniform magnetic field, putting the calcium alginate gel particles into deionized water at 35 ℃, ultrasonically dispersing for 7min, and naturally cooling; mixing the strains cultured in the step of culturing the microorganisms, putting the mixture into the calcium alginate gel particle solution, keeping the temperature within 22 ℃, and keeping the temperature for 25min.
The concentration of the calcium alginate gel particles is 6 percent.
The strength of the uniform magnetic field is 300mt.
The immobilization is carried out by dripping 4 percent of CaCl into the mixture of the calcium alginate gel particles and each strain under the condition of applying uniform magnetic field 2 Stirring the solution at 7RPM for 2h, and filtering out the calcium alginate gel particles; putting the calcium alginate gel particles into a normal-temperature 0.03% hexamethylene diamine solution, stirring for 45min, and filtering out the calcium alginate gel particles; crosslinking with 0.2% glutaraldehyde for 12min, and filtering out the calcium alginate gel particles; spraying 0.3% chitosan solution on the calcium alginate gel particles; the calcium alginate gel particles were rinsed 2 times with deionized water.
The chitosan solution is prepared by dissolving quantitative chitosan in 1% acetic acid aqueous solution.
The strength of the uniform magnetic field is 500mt.
And (3) activating, namely placing the calcium alginate gel particles in an environment at 13 ℃, applying a uniform magnetic field with the strength of 500mt, increasing the magnetic field strength of 50mt every 30min until the magnetic field strength is 1000mt, and keeping for 2h to obtain the immobilized microorganism particles.
And the sewage treatment step of contacting the immobilized microorganism particles with sewage, carrying out aeration treatment in a stirring state until the quality of the sewage reaches a discharge standard, filtering out the immobilized microorganism particles, and discharging the sewage after the treatment.
Example 5
The sewage treatment test was carried out by the method for treating sewage by the fluidized bed with immobilized microorganism particles by the embedding method described in examples 1 to 4, and the comparative tests 1 to 3 were set, and the water quality indexes after the sewage treatment by the method for treating sewage by the fluidized bed with immobilized microorganism particles by the embedding method described in examples 1 to 4 and comparative examples 1 to 3 were compared.
Production sewage of a certain production enterprise is selected as a test sample, and the water quality indexes of the test sample are shown in the following table:
comparative example 1: the method for treating sewage by using the embedding method immobilized microorganism particle fluidized bed in the embodiment 3 is different from the following steps: the step of 'carrier treatment' is omitted, and the conventional calcium alginate gel particles are used as carriers.
Comparative example 2: the method for treating sewage by using the embedding method immobilized microorganism particle fluidized bed in the embodiment 3 is different from the following steps: in the "embedding step" and the "immobilizing step", a uniform magnetic field is not applied.
Comparative example 3: the method for treating sewage by using the embedding method immobilized microorganism particle fluidized bed in the embodiment 3 is different from the following steps: the "activation step" step is omitted.
The comparison of the detection indexes of the treated wastewater quality of the examples 1 to 4 and the comparative examples 1 to 3 is shown in the following table:
in addition, through detection, the immobilized microorganism particle fluidized bed sewage treatment method of the embedding method has high relative activity of the immobilized microorganism, and the relative activity reaches 69.3 to 74.2 percent; the mechanical strength of the immobilized microbial particles is high, and is improved by 15.3 to 21.4 percent compared with the mechanical strength of the immobilized microbial particles adopting the existing calcium alginate gel particles; the chemical stability of the immobilized microorganism particles is good, and the immobilized microorganism particles are placed in 0.05mol/L phosphate buffer solution and do not change for 170 hours.
All percentages used in the present invention are mass percentages unless otherwise indicated.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (1)
1. An embedding method immobilized microorganism particle fluidized bed sewage treatment method is characterized by comprising the following steps: microorganism culture, carrier treatment, embedding, immobilization, activation and sewage treatment;
the microorganism culture is carried out, 10 to 15 parts of bacillus subtilis, 3 to 7 parts of actinomycetes, 10 to 15 parts of thiobacillus denitrificans, 15 to 20 parts of pseudomonas stutzeri and 5 to 10 parts of bacillus coagulans are respectively put into a sterile culture medium, culture solution is added, the stirring speed is 30 to 60RPM, and the culture time is 24 to 48 hours;
treating the carrier, wherein the carrier is calcium alginate gel particles; adding the calcium alginate gel particles into saturated sodium carbonate-water solution with the volume of 5 times, heating to 25-35 ℃, and stirring at 60RPM for 20min; continuously heating to 35-45 ℃, and stirring at 100RPM for 20min; continuously heating to 50 ℃, preserving heat and standing for 1h; filtering out the particles; washing the granules for 3-5 min by using sufficient 2% acetic acid; then washing with sufficient deionized water;
embedding, namely putting the calcium alginate gel particles treated by the carrier into deionized water at the temperature of 30-40 ℃ under the condition of applying a uniform magnetic field, performing ultrasonic dispersion for 5-10 min, and naturally cooling; mixing the strains cultured in the step of microbial culture, putting the mixture into dispersion liquid, keeping the temperature within the range of 20-25 ℃, and keeping the temperature for 20-30 min;
in the embedding process, a uniform magnetic field with the strength of 200-400 mt is applied;
the immobilization is carried out by dripping 3-5% of CaCl into the mixture of the embedded calcium alginate gel particles and each strain under the condition of applying uniform magnetic field 2 Stirring the solution for 2 to 2.5 hours at 5 to 10RPM, and filtering out particles; putting the particles into a normal-temperature 0.02-0.04% hexamethylene diamine solution, stirring for 30-60 min, and filtering out the particles; crosslinking for 10-15 min by adopting 0.1-0.3% of glutaraldehyde, and filtering out particles; spraying 0.3% chitosan solution on the particles; washing with deionized water;
the chitosan solution is prepared by dissolving quantitative chitosan in 1% acetic acid aqueous solution; the intensity of the immobilized uniform magnetic field is 400-600 mt;
and (3) activating, namely placing the immobilized calcium alginate gel particles in an environment of 12-15 ℃, applying a uniform magnetic field with the strength of 400-600 mt, increasing the magnetic field strength by 50mt every 30min until the magnetic field strength is 1000mt, and keeping for 2h to obtain the immobilized microorganism particles.
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