JPH08308570A - Production of organism carrier for immobilizing fine particle of magnetic material - Google Patents

Abstract

PURPOSE: To obtain the subject carrier which is inexpensive, excellent in strength, useful in the fields of fermentation, food, chemistry, biochemistry, environment, etc., for supporting a biocatalyst by adding fine particles of a paramagnetic material to a porous ceramic, molding the mixture into a molded compact and backing it. CONSTITUTION: A porous ceramic is mixed with 10-90wt.% of fine particles of a paramagnetic material having 0.01-100μm particle diameter, molded into a proper shape and baked at 1,100-1,300 deg.C to give the objective carrier for immobilizing magnetic material of fine particles, relatively inexpensive, strong in mechanical strength, increasing proliferation of a microorganism, etc., by effects caused by a magnetic field, providing a highly biological activity in supporting a bacterium, an enzyme, etc., on the carrier, useful for a biocatalyst excellent in physics, chemistry and biochemistry in the field of fermentation, food, chemistry, biochemistry, environment, etc.

Description

Description: BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention provides an immobilized biocatalyst suitable for biological use containing a fine particle paramagnetic substance.
The present invention relates to a method for producing a bioimmobilization carrier which is relatively inexpensive, has high mechanical strength, and has biological activity and which is excellent in biocatalyst. [0002] Currently, as a method for immobilizing a biocatalyst, a carrier binding method in which a biocatalyst is bound to an insoluble carrier to immobilize it, and a functional group in an enzyme protein or a cell wall of a microbial cell by a reagent are used. There are cross-linking method that forms and immobilizes cell membranes and cross-links, and entrapment method that encloses biocatalyst in gel of natural polymer or synthetic polymer such as protein and cellulose, but physical adsorption method of carrier binding method. Has a weak bond between the carrier and the biocatalyst and has low biological activity. There are covalent bonding method, ionic bonding method, and physical adsorption method in the carrier binding method. In the physical binding method of the carrier binding method, the binding between the carrier and the biocatalyst is weak and desorption is difficult. They are often separated, which is the cause of the poor biological activity. To solve these problems, by using a biological carrier containing a fine particle paramagnetic material in a porous ceramic material as a carrier material, a biologically active substance can be supported and high biological activity can be obtained. Can be used for immobilized biocatalysts. In the present invention, various disclosures have been made that cells, which are a mixture of various components, have an effect by a magnetic field, for example (Candida albica).
50-900G was given to 5 kinds of microorganisms such as ns), and the growth of the microorganisms increased from 0 to 200G, especially about 30% at 200G, but conversely the growth was suppressed above 200G. I'm standing. In addition, the use of magnetic powder for immobilization of microbial proteins was announced, and it was revealed that magnetic substances are effective in inhibiting microbial growth. According to the present invention, by incorporating a magnetic substance (fine particle paramagnetic substance) having such an effect into the porous ceramics to cause the fine particle paramagnetic substance contained therein to adsorb and adsorb microorganisms, the binding of the biocatalyst can be achieved. It was found that it was excellent, the immobilization was stable, and the microorganisms also grew. The biological carrier for immobilization of fine particle paramagnetic material of the present invention is obtained by including fine particle paramagnetic material in porous ceramics and molding it into an appropriate shape, and then firing this to obtain a biological carrier for immobilization. INDUSTRIAL APPLICABILITY The biological carrier for immobilizing fine particle paramagnetic material produced according to the present invention uses a bacterium, an enzyme or the like, and immobilizes a biocatalyst in the fields of fermentation, food, chemical industry, environmental purification, etc. Due to its excellent (adsorption), it can be used in various applications and new technologies can be developed. Example 1 According to the present invention A biocarrier for immobilization of fine particle paramagnetic material produced by blending raw materials of [Table 2] Using the biological carrier for immobilizing porous ceramics produced by blending the above raw materials, the prior application (Japanese Patent Application No. 5-245662), the adsorptivity is compared. Yeast, (Candida arbore
a) was cultivated, the bacterial cells were collected, and adsorption was compared. The results are shown in [Table 3]. [Table 4] Noto street.

   ─────────────────────────────────────────────────── ─── Continued front page    (72) Inventor Kunihiko Odaka             1-2-8 Kashiihama, Higashi-ku, Fukuoka-shi, Fukuoka             No. 502 Limited Company Agricultural Technology Institute (72) Inventor Sakaguchi Nami             18-81, Chokai, Yamauchi-cho, Kishima-gun, Saga Prefecture

Claims (1)

Claim: What is claimed is: 1. A bioimmobilization carrier comprising a fine particle paramagnetic material in porous ceramics. 2. The particle diameter of the fine particle paramagnetic material according to claim 1, which is from 0.01 to
It is 100 μm. 3. The content of the fine particle paramagnetic material according to claim 2 in a weight ratio of 10.
~ 90%. 4. The calcination temperature of the bioimmobilization carrier according to claim 1 is 1100.
~ 1300 ° C.