CN111999440B - Natural enemy insect toxicity test device - Google Patents
Natural enemy insect toxicity test device Download PDFInfo
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- CN111999440B CN111999440B CN202010868927.2A CN202010868927A CN111999440B CN 111999440 B CN111999440 B CN 111999440B CN 202010868927 A CN202010868927 A CN 202010868927A CN 111999440 B CN111999440 B CN 111999440B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
- A01M1/04—Attracting insects by using illumination or colours
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/20—Poisoning, narcotising, or burning insects
Abstract
The invention discloses a natural enemy insect toxicity test device which comprises a base and a test assembly arranged on the base, wherein the test assembly comprises a test mechanism and a dosing mechanism arranged at the bottom of the test mechanism, an insect cavity is arranged at the inner side of the test mechanism, a plurality of sample inlets are formed in the inner wall of the insect cavity along the circumferential direction, test channels are arranged in the test mechanism, the test channels correspond to the sample inlets one by one, and the test channels are not communicated with one another; a plurality of drug feeding holes are formed in the outer wall of the drug feeding mechanism at intervals along the circumferential direction, any test channel is communicated with at least one drug feeding hole, and one end of the drug feeding mechanism is communicated with a drug box; be provided with the support on the base, the cooperation is connected with light source spare on the support, light source spare outside interval is provided with a plurality of lamp pearls, the lamp pearl with introduction port position one-to-one, the light source that shines of lamp pearl can shine to in the introduction port, the light source luminance of lamp pearl can be adjusted.
Description
Technical Field
The invention relates to a toxicity test, in particular to a toxicity test device for natural enemy insects.
Background
The acute toxicity test is for the medicine, and the magnitude of the acute toxicity of the medicine is measured by half lethal dose, wherein the half lethal dose is the dose of a certain medicine which causes the insect which is the natural enemy of the test to die generally, and as the half lethal dose is the most sensitive point on a dose response curve and has the advantages of easy measurement, accuracy and good repeatability, the half lethal dose is taken as a safety index for the use of the medicine, the toxicity test is carried out on the natural enemy insect in the test process, and the death condition is taken as an evaluation index to judge the acute toxicity of a certain biological material.
Most of the existing natural enemy insect toxicity tests are to wrap finger-shaped tubes where insects are located with black cloth, quickly butt-joint transparent target finger-shaped tubes in the dark, then turn on a light source, add a set dose of medicament into an index tube after the insects enter the target finger-shaped tubes, and then observe and calculate the death number of the insects so as to carry out toxicity tests, wherein the mode test precision is poor and the operation difficulty is high.
Disclosure of Invention
The invention aims to provide a natural enemy insect toxicity test device to overcome the defects in the prior art.
In order to solve the technical problems, the technical scheme of the invention is as follows: a natural enemy insect toxicity test device, which comprises a base and a test component arranged on the base,
the testing assembly comprises a testing mechanism and a dosing mechanism arranged at the bottom of the testing mechanism, an insect cavity is arranged at the inner side of the testing mechanism, a plurality of sample inlets are formed in the inner wall of the insect cavity along the circumferential direction, testing channels are arranged in the testing mechanism, the testing channels correspond to the sample inlets one to one, and the testing channels are not communicated with one another;
a plurality of drug feeding holes are formed in the outer wall of the drug feeding mechanism at intervals along the circumferential direction, any test channel is communicated with at least one drug feeding hole, and one end of the drug feeding mechanism is communicated with a drug box;
be provided with the support on the base, the cooperation is connected with light source spare on the support, light source spare outside interval is provided with a plurality of lamp pearls, the lamp pearl with introduction port position one-to-one, the light source that shines of lamp pearl can shine to in the introduction port, the light source luminance of lamp pearl can be adjusted.
Preferably, the support and the base are fixedly connected through a bolt, a reinforcing rib is arranged on one side of the support, and a preset angle is formed between the reinforcing rib and the support.
Preferably, the top of the testing mechanism is provided with a plurality of light supplementing pieces, at least one light supplementing piece is arranged at the top of the testing channel, a light supplementing lamp and at least one probe are arranged at the bottom of the light supplementing piece, the bending angle of the light supplementing lamp is the same as that of the testing channel, and the probe is used for identifying the internal state of the testing channel.
Preferably, the number of the test channels is 3, the bending directions of the 3 test channels are the same, and a comparison area is formed in a parallel overlapping area between any two test channels, and a comparison test can be formed in the comparison area.
Preferably, a temporary holding cavity is arranged at the bottom of the medication administration mechanism, a support rod is arranged at the bottom of the temporary holding cavity, the support rod is hollow, and a gasket is arranged at the bottom of the support rod.
Preferably, branch one side is connected with the medicament pipe, medicament pipe one end is connected with the medicament case through the flange, the medicament case top is provided with the closing cap, the closing cap is detachable construction.
Preferably, the base top is provided with a plurality of baffles, the baffle with connect through the mounting between the base, a plurality of baffles form and surround the space, the test subassembly is located surround the space inboard, surround the space inner wall and be provided with the light-absorbing layer.
Preferably, four corners of the bottom of the base are provided with damping mechanisms, the bottoms of the damping mechanisms are connected with rollers in a matching mode, and at least one roller is provided with a locking mechanism.
Preferably, a motor is arranged at the top of the support, and the motor is connected with an adjusting mechanism in a matching manner, and the adjusting mechanism can rotate.
Preferably, the bottom of the adjusting mechanism is connected with a control mechanism in a matching manner, one side of the control mechanism is connected with a suspension, the end part of the suspension is fixedly connected with a light source, and the suspension can be adjusted in height in a telescopic manner through the adjusting mechanism.
The invention solves the defects in the background technology, and has the following beneficial effects:
(1) according to the invention, the insects are guided to the sample inlet through phototaxis of the insects, then the light supplementing piece is used for supplementing light to the test channel, so that the whole test channel is filled with the light source, the bottom of the test channel is dosed through the dosing hole, so that the pesticide is spread on the bottom of the test channel, the actual environment of the insects exposed by the chemical pesticide can be better simulated through the mode, and the test effect is better.
(2) The test mechanism is internally provided with a plurality of test channels which are separated from each other to form a multi-sample test, the test precision is higher, a coincidence region with a certain length is arranged between any two test channels, the coincidence region is a contrast region, the two test channels in the contrast region can form a contrast test, and the toxicity test of insects is carried out by measuring the length of the insects moving along the test channels and calculating the half death amount.
(3) The lamp beads on the light source piece correspond to the sample inlets in a one-to-one mode, insects in the insect cavities can be led into different test channels through the light sources irradiated by the lamp beads, the light source piece is matched with the sample inlets through adjusting the position of the light source piece by the adjusting mechanism, and the insects can be better guided to enter the sample inlets.
(4) The medicine feeding holes are distributed on the outer wall of the medicine feeding mechanism at intervals along the circumferential direction, so that the medicine can enter from the bottom of the test channel, the depth of the reagent inside the test channel or the amount of the reagent can be accurately controlled, and the toxicity test can be better carried out.
(5) The test mechanism is located in a surrounding space formed by the baffles, and the inner wall of the surrounding space absorbs an external light source through the light absorption layer, so that the surrounding space is in a relatively dark environment, and the guide of the light source piece is facilitated.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments described in the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic partial perspective view of the present invention;
FIG. 2 is a schematic view of the structure of the chemical tank and the testing mechanism of the present invention;
FIG. 3 is a schematic view of another angle of the test apparatus of the present invention;
FIG. 4 is a schematic structural diagram of a testing mechanism of the present invention;
FIG. 5 is a schematic bottom structure of the testing mechanism of the present invention;
FIG. 6 is a schematic view of the distribution of the administration holes of the present invention;
FIG. 7 is a schematic illustration of a test channel distribution according to the present invention;
specifically, the device comprises a motor 1, a motor 2, an adjusting mechanism 3, a support 4, a control mechanism 5, a reinforcing rib 6, a sealing cover 7, a chemical box 8, a flange 9, a chemical tube 10, a base 11, an insect cavity 12, a fixing piece 13, a roller 14, a damping mechanism 15, a sample inlet 16, a testing mechanism 17, a light supplementing piece 18, a light source piece 19, a baffle plate 20, a lamp bead 21, a suspension frame 22, a temporary holding cavity 23, a support rod 24, a gasket 25, a chemical dosing mechanism 26, a chemical dosing hole 27 and a testing channel.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described herein, and therefore the scope of the present invention is not limited by the specific embodiments disclosed below.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on those shown in the drawings, and are used merely for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be taken as limiting the scope of the present invention. Furthermore, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the invention, the meaning of "a plurality" is two or more unless otherwise specified.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations.
As shown in FIGS. 1-3, the present invention discloses a local three-dimensional structure schematic diagram of a natural enemy insect toxicity test device;
in particular to a natural enemy insect toxicity test device which comprises a base 10 and a test component arranged on the base 10,
the testing component comprises a testing mechanism 16 and a dosing mechanism 25 arranged at the bottom of the testing mechanism 16, an insect cavity 11 is arranged on the inner side of the testing mechanism 16, a plurality of sample inlets 15 are formed in the inner wall of the insect cavity 11 along the circumferential direction, testing channels 27 are arranged in the testing mechanism 16, the testing channels 27 correspond to the sample inlets 15 one by one, and the testing channels 27 are not communicated with each other;
a plurality of drug feeding holes 26 are formed in the outer wall of the drug feeding mechanism 25 at intervals along the circumferential direction, any test channel 27 is communicated with at least one drug feeding hole 26, and one end of the drug feeding mechanism 25 is communicated with the drug box 7;
be provided with support 3 on the base 10, the cooperation is connected with light source 18 on the support 3, and light source 18 outside interval is provided with a plurality of lamp pearls 20, and lamp pearl 20 and 15 position one-to-one in the inlet, the light source of lamp pearl 20 can shine to the inlet 15 in, and the light source luminance of lamp pearl 20 can be adjusted.
It should be noted that, the insects are guided to the injection port 15 through the phototaxis of the insects, the insects can include trichogrammatid or ladybug, but not limited to the two types, the skilled in the art can change the insect species according to the actual test, then the light supplement part 17 is used for supplementing light to the test channel 27, so that the whole test channel 27 is filled with the light source, the bottom of the test channel 27 is used for feeding the chemical through the chemical feeding hole 26, the chemical is spread over the bottom of the test channel 27, the actual environment of the insects exposed by the chemical pesticide can be better simulated through the method, and the test effect is better.
The test mechanism 16 is internally provided with a plurality of test channels 27, the test channels 27 are mutually separated to form a multi-sample test, and the test precision is high.
According to the embodiment of the invention, the bracket 3 is fixedly connected with the base 10 through bolts, the reinforcing rib 5 is arranged on one side of the bracket 3, and a preset angle is arranged between the reinforcing rib 5 and the bracket 3.
FIGS. 4-5 are schematic structural views of the testing mechanism 16 of the present invention;
according to the embodiment of the invention, the top of the testing mechanism 16 is provided with a plurality of light supplementing pieces 17, the top of any testing channel 27 is provided with at least one light supplementing piece 17, the bottom of each light supplementing piece 17 is provided with a light supplementing lamp and at least one probe, the bending angle of each light supplementing lamp is the same as that of the testing channel 27, and the probes are used for identifying the internal state of the testing channel 27.
It should be noted that the lamp beads 20 on the light source 18 correspond to the sample inlets 15 one by one, the insects in the insect cavity 11 can be introduced into different test channels 27 by the light source irradiated by the lamp beads 20, and the position of the light source 18 is adjusted by the adjusting mechanism 2, so that the light source 18 is matched with the sample inlet 15, and the insects can be better guided to enter the sample inlet 15.
As shown in FIG. 7, the present invention discloses a schematic distribution of test channels 27;
according to the embodiment of the invention, the number of the test channels 27 is 3, the bending directions of the 3 test channels 27 are the same, and the parallel overlapping area between any two test channels 27 forms a contrast area, and a contrast test can be formed in the contrast area.
It should be noted that there is a certain length of overlapping region between any two test channels 27, which is a comparison region, and the two test channels 27 in the comparison region can form a comparison test, and the toxicity test of the insect is performed by measuring the length and half the death amount of the insect moving along the test channels 27.
As shown in fig. 6, the present invention discloses a distribution diagram of the administration holes 26;
according to the embodiment of the invention, the drug administration mechanism 25 is provided with a temporary storage cavity 22 at the bottom, a support rod 23 is arranged at the bottom of the temporary storage cavity 22, the support rod 23 is hollow, and a gasket 24 is arranged at the bottom of the support rod 23.
The administration holes 26 are circumferentially spaced on the outer wall of the administration mechanism 25, so that the drug can enter from the bottom of the test channel 27, and the reagent depth or the reagent amount inside the test channel 27 can be accurately controlled, thereby enabling a better toxicity test.
According to the embodiment of the invention, one side of the support rod 23 is connected with the medicament tube 9, one end of the medicament tube 9 is connected with the medicament box 7 through the flange 8, the top of the medicament box 7 is provided with the sealing cover 6, and the sealing cover 6 is of a detachable structure.
According to the embodiment of the invention, a plurality of baffles 19 are arranged on the top of the base 10, the baffles 19 are connected with the base 10 through fixing pieces 12, a surrounding space is formed by the plurality of baffles 19, the test assembly is positioned on the inner side of the surrounding space, and a light absorption layer is arranged on the inner wall of the surrounding space.
It should be noted that the testing mechanism 16 is located in the enclosed space formed by the plurality of baffles 19, and the inner wall of the enclosed space absorbs the external light source through the light absorbing layer, so that the enclosed space is in a relatively dark environment, which is favorable for guiding the light source element 18.
According to the embodiment of the invention, four corners of the bottom of the base 10 are provided with the damping mechanisms 14, the bottoms of the damping mechanisms 14 are connected with the rollers 13 in a matching manner, and at least one roller 13 is provided with the locking mechanism.
According to the embodiment of the invention, the top of the bracket 3 is provided with the motor 1, the motor 1 is connected with the adjusting mechanism 2 in a matching way, and the adjusting mechanism 2 can rotate.
According to the embodiment of the invention, the bottom of the adjusting mechanism 2 is connected with the control mechanism 4 in a matching manner, one side of the control mechanism 4 is connected with the suspension 21, the end part of the suspension 21 is fixedly connected with the light source part 18, and the suspension 21 can be adjusted in height in a telescopic manner through the adjusting mechanism 2.
According to the invention, the insects are guided to the sample inlet 15 through phototaxis of the insects, then the light supplementing piece 17 is used for supplementing light to the test channel 27, so that the whole test channel 27 is filled with the light source, the medicine feeding is carried out at the bottom of the test channel 27 through the medicine feeding hole 26, the medicine is spread at the bottom of the test channel 27, the actual environment of the insects exposed by chemical pesticide can be better simulated through the mode, and the test effect is better.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (6)
1. A natural enemy insect toxicity test device comprises a base and a test component arranged on the base, and is characterized in that,
the testing assembly comprises a testing mechanism and a dosing mechanism arranged at the bottom of the testing mechanism, an insect cavity is arranged at the inner side of the testing mechanism, a plurality of sample inlets are formed in the inner wall of the insect cavity along the circumferential direction, testing channels are arranged in the testing mechanism, the testing channels correspond to the sample inlets one to one, and the testing channels are not communicated with one another;
a plurality of drug feeding holes are formed in the outer wall of the drug feeding mechanism at intervals along the circumferential direction, any test channel is communicated with at least one drug feeding hole, and one end of the drug feeding mechanism is communicated with a drug box;
the base is provided with a support, the support is connected with a light source piece in a matching manner, a plurality of lamp beads are arranged on the outer side of the light source piece at intervals, the lamp beads correspond to the positions of the sample inlets one by one, the irradiation light sources of the lamp beads can irradiate into the sample inlets, and the light source brightness of the lamp beads can be adjusted;
the bracket is fixedly connected with the base through a bolt, a reinforcing rib is arranged on one side of the bracket, and a preset angle is formed between the reinforcing rib and the bracket;
a temporary holding cavity is formed in the bottom of the dosing mechanism, a support rod is arranged at the bottom of the temporary holding cavity, the support rod is hollow, and a gasket is arranged at the bottom of the support rod;
one side of the supporting rod is connected with a medicament tube, one end of the medicament tube is connected with a medicament box through a flange, the top of the medicament box is provided with a sealing cover, and the sealing cover is of a detachable structure;
the testing mechanism top is provided with a plurality of light supplementing piece, and is arbitrary the test channel top is provided with at least one light supplementing piece, light supplementing piece bottom is provided with light supplementing lamp and at least one probe, light supplementing lamp bending angle with test channel bending angle is the same, the probe is used for discerning test channel internal state.
2. The natural enemy insect toxicity test device of claim 1, wherein the test channels are 3, the bending directions of the 3 test channels are the same, and the areas of juxtaposition and coincidence between any two test channels form a contrast area.
3. The natural enemy insect toxicity test device as claimed in claim 1, wherein a plurality of baffles are arranged on the top of the base, the baffles are connected with the base through fixing parts, a surrounding space is formed by the baffles, the test assembly is located on the inner side of the surrounding space, and a light absorption layer is arranged on the inner wall of the surrounding space.
4. The natural enemy insect toxicity test device of claim 1, wherein four corners of the bottom of the base are provided with damping mechanisms, the bottoms of the damping mechanisms are connected with rollers in a matching mode, and at least one roller is provided with a locking mechanism.
5. The natural enemy insect toxicity test device as claimed in claim 1, wherein a motor is arranged on the top of the support, and is connected with an adjusting mechanism in a matching manner, and the adjusting mechanism can rotate.
6. The natural enemy insect toxicity test device as claimed in claim 5, wherein a control mechanism is connected to the bottom of the adjusting mechanism in a matching manner, a suspension is connected to one side of the control mechanism, a light source piece is fixedly connected to the end of the suspension, and the suspension can be adjusted in height in a telescopic manner through the adjusting mechanism.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010868927.2A CN111999440B (en) | 2020-08-25 | 2020-08-25 | Natural enemy insect toxicity test device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010868927.2A CN111999440B (en) | 2020-08-25 | 2020-08-25 | Natural enemy insect toxicity test device |
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| CN111999440A CN111999440A (en) | 2020-11-27 |
| CN111999440B true CN111999440B (en) | 2022-04-26 |
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