CN111996145A - Preparation process and use method of microbial strain for degrading kitchen waste - Google Patents
Preparation process and use method of microbial strain for degrading kitchen waste Download PDFInfo
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- CN111996145A CN111996145A CN202010887156.1A CN202010887156A CN111996145A CN 111996145 A CN111996145 A CN 111996145A CN 202010887156 A CN202010887156 A CN 202010887156A CN 111996145 A CN111996145 A CN 111996145A
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- 239000010806 kitchen waste Substances 0.000 title claims abstract description 55
- 238000002360 preparation method Methods 0.000 title claims abstract description 35
- 230000000593 degrading effect Effects 0.000 title claims abstract description 28
- 230000000813 microbial effect Effects 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 24
- 241000894006 Bacteria Species 0.000 claims abstract description 47
- 230000001580 bacterial effect Effects 0.000 claims abstract description 22
- 241000186361 Actinobacteria <class> Species 0.000 claims abstract description 17
- 150000002632 lipids Chemical class 0.000 claims abstract description 17
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 12
- 230000015556 catabolic process Effects 0.000 claims abstract description 8
- 238000006731 degradation reaction Methods 0.000 claims abstract description 8
- 108090000790 Enzymes Proteins 0.000 claims abstract description 7
- 102000004190 Enzymes Human genes 0.000 claims abstract description 7
- 241000108664 Nitrobacteria Species 0.000 claims abstract description 7
- 241000589516 Pseudomonas Species 0.000 claims abstract description 7
- 230000003213 activating effect Effects 0.000 claims abstract description 7
- 150000001875 compounds Chemical class 0.000 claims abstract description 7
- 150000004676 glycans Chemical class 0.000 claims abstract description 7
- 235000015097 nutrients Nutrition 0.000 claims abstract description 7
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 7
- 239000005017 polysaccharide Substances 0.000 claims abstract description 7
- 238000000855 fermentation Methods 0.000 claims description 25
- 230000004151 fermentation Effects 0.000 claims description 25
- 239000002068 microbial inoculum Substances 0.000 claims description 20
- 239000001963 growth medium Substances 0.000 claims description 16
- 238000001179 sorption measurement Methods 0.000 claims description 15
- 238000003756 stirring Methods 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 230000000694 effects Effects 0.000 claims description 12
- 239000002609 medium Substances 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 12
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 10
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 10
- 244000005700 microbiome Species 0.000 claims description 9
- 238000010899 nucleation Methods 0.000 claims description 7
- 229920001817 Agar Polymers 0.000 claims description 6
- 239000008272 agar Substances 0.000 claims description 6
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims description 5
- 241000228245 Aspergillus niger Species 0.000 claims description 5
- 244000063299 Bacillus subtilis Species 0.000 claims description 5
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 5
- 240000007594 Oryza sativa Species 0.000 claims description 5
- 235000007164 Oryza sativa Nutrition 0.000 claims description 5
- 239000001888 Peptone Substances 0.000 claims description 5
- 108010080698 Peptones Proteins 0.000 claims description 5
- 244000062793 Sorghum vulgare Species 0.000 claims description 5
- 241000209140 Triticum Species 0.000 claims description 5
- 235000021307 Triticum Nutrition 0.000 claims description 5
- 240000008042 Zea mays Species 0.000 claims description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 5
- 235000010216 calcium carbonate Nutrition 0.000 claims description 5
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 5
- 229910052799 carbon Inorganic materials 0.000 claims description 5
- 235000013339 cereals Nutrition 0.000 claims description 5
- 235000005822 corn Nutrition 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 239000004021 humic acid Substances 0.000 claims description 5
- 230000004060 metabolic process Effects 0.000 claims description 5
- 235000019713 millet Nutrition 0.000 claims description 5
- 229910052757 nitrogen Inorganic materials 0.000 claims description 5
- 235000019319 peptone Nutrition 0.000 claims description 5
- 235000009566 rice Nutrition 0.000 claims description 5
- 150000003839 salts Chemical class 0.000 claims description 5
- 230000004083 survival effect Effects 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 239000000463 material Substances 0.000 claims 1
- 239000002699 waste material Substances 0.000 abstract description 2
- 238000010790 dilution Methods 0.000 description 7
- 239000012895 dilution Substances 0.000 description 7
- 239000003085 diluting agent Substances 0.000 description 4
- 235000013305 food Nutrition 0.000 description 3
- 239000004519 grease Substances 0.000 description 2
- 239000010813 municipal solid waste Substances 0.000 description 2
- 239000006916 nutrient agar Substances 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 210000003278 egg shell Anatomy 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 235000021190 leftovers Nutrition 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 241001446247 uncultured actinomycete Species 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B2101/00—Type of solid waste
- B09B2101/018—Animal meals
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- Life Sciences & Earth Sciences (AREA)
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Virology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Environmental & Geological Engineering (AREA)
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Abstract
The invention discloses a preparation process of a microbial strain for degrading kitchen waste and a use method thereof, relates to the technical field of waste degradation, and aims to solve the problem that the total number of bacterial colonies tends to a normal value due to the fact that more cost is required to be added by a user under the condition that the total number of the bacterial colonies is low in the existing preparation process of the microbial strain for degrading kitchen waste. The GANDEW-MIX COD removing bacteria are a compound bacteria system consisting of 50 kinds of bacteria of 6 genera, can adapt to different kitchen waste environments, and mainly comprise nitrobacteria, denitrifying bacteria, bacillus, pseudomonas, activating enzyme, polysaccharide and other nutrients; the actinomycetes are used for removing the odor in the kitchen waste; GANDEW-LIPA lipid decomposing bacteria are used for removing the lipid in the kitchen waste.
Description
Technical Field
The invention relates to the technical field of garbage degradation, in particular to a preparation process of a microbial strain for degrading kitchen garbage and a use method thereof.
Background
The kitchen waste refers to waste generated in daily life and food processing, food service, unit catering and other activities of residents, and comprises abandoned vegetable leaves, leftovers, fruit peels, egg shells, tea leaves, bones and the like, and the main sources of the kitchen waste are household kitchens, restaurants, dining halls, markets and other industries related to food processing.
The problem that the total number of bacterial colonies tends to be normal value due to the fact that the total number of bacterial colonies is low in the existing preparation process of the microbial bacteria for degrading the kitchen waste is caused by the fact that a user needs to increase more cost; therefore, the existing requirements are not met, and a preparation process and a use method of the microbial strain for degrading the kitchen waste are provided.
Disclosure of Invention
The invention aims to provide a preparation process of a microbial strain for degrading kitchen waste and a use method thereof, and aims to solve the problem that the total number of bacterial colonies is low in the preparation process of the existing microbial strain for degrading kitchen waste in the background technology, so that more cost needs to be added by a user to enable the total number of the bacterial colonies to approach a normal value.
In order to achieve the purpose, the invention provides the following technical scheme: a preparation process of microbial strains for degrading kitchen waste comprises the following steps of:
removing 20-30% of bacteria from GANDEW-MIX COD;
8% -15% of GANDEW-LIPA lipid decomposing bacteria;
5% -10% of actinomycetes;
15 to 35 percent of bacillus subtilis
15 to 35 percent of Aspergillus niger.
Preferably, the GANDEW-MIX COD removing bacteria are a compound bacterial system consisting of 50 kinds of bacteria in 6 genera, and can adapt to different kitchen waste environments, and the GANDEW-MIX COD removing bacteria mainly comprise nitrobacteria, denitrifying bacteria, bacillus, pseudomonas, activating enzyme, polysaccharide and other nutrients; the actinomycetes are used for removing the odor in the kitchen waste; GANDEW-LIPA lipid decomposing bacteria are used for removing the lipid in the kitchen waste.
Preferably, all the equipment is subjected to steam sterilization before preparation, the process parameters are 121-125 ℃, 0.105-0.170 MPa and 0.5-1.2 h, and equipment such as a seed tank, a fermentation tank, a dryer, a stirring mechanism and the like is required in the preparation process.
Preferably, the culture medium of the seeding tank ingredient seeding tank takes natural agricultural products such as rice, millet, corn, bran, wheat grains and the like as the culture medium, the culture of the mould is generally 25-28 ℃, the culture time is 4-14, and the total number of bacterial colonies is improved by 75-90%.
Preferably, the culture medium is an agar slant culture medium which contains components suitable for spores, such as bran, pea extract, peptone, some inorganic salts and the like, the carbon source is about 1 percent, the nitrogen source is not more than 0.5 percent, the culture temperature of the actinomycete slant is 28-37 ℃, the culture time is 5-14 days, and the total number of colonies is increased by 75-90 percent.
Preferably, the fermentation of the fermentation tank is kept at 25-35 ℃ for 24-36h, and the process performs microscopic examination on the fermentation tank: the shape and density of the thallus are more than or equal to 80 percent.
Preferably, the stirring mechanism performs adsorption stirring after fermentation is completed, and adds light CaCO3 or humic acid of 80 meshes, wherein the feeding amount of an adsorption medium is 1-3:1, and the water content after adsorption is 25-50%.
Preferably, the temperature of a drying heat medium of the dryer is 100-120 ℃, and the moisture of a dried product is less than 10 percent, so that a powdery microbial inoculum is formed, and the degradation efficiency is improved by 50-75 percent.
Preferably, the content of the microorganism bacteria in the powdery microbial inoculum is 25-200 hundred million/g.
Preferably, the use method of the microbial strain for degrading the kitchen waste comprises the following steps:
the method comprises the following steps: when the powdery microbial inoculum is used, direct sunlight is avoided, so that the activity of bacillus in the powdery microbial inoculum, such as propagation, metabolism and the like, can be influenced and even lose activity easily caused by the direct sunlight;
step two: when in use, the water content is kept to be 60-70% because the water content suitable for the survival of the strains is kept;
step three: waiting for the microorganism to degrade, wherein the time is about 24-68 h according to different kitchen wastes and different contents.
Compared with the prior art, the invention has the beneficial effects that:
1. the invention detects the bacterial colony generated by the preparation process, and the operation method comprises the following steps: sampling 25g by aseptic operation, placing in a sterilized glass bottle of 225ml sterilized normal saline or other diluent, grinding fully to obtain 1:10 uniform diluent, adding the diluent into the solid sample, and treating in a sterilized homogenizer at 8000-10000r/min for 1min to obtain 1:10, taking another 1ml of sterilization pipette, preparing 10 times of gradually increased dilution according to the above operation sequence, selecting 2-3 appropriate dilutions, respectively preparing 10 times of gradually increased dilution, simultaneously sucking the dilution pipette to transfer 1ml of dilution into a sterilization dish, and preparing two plates for each dilution. The nutrient agar medium cooled to 46 ℃ is poured into a dish of about 15ml, and the dish is rotated and mixed uniformly. At the same time, the nutrient agar medium was poured into a sterile plate to which 1ml of the diluent (without sample) was added as a blank. After the agar is solidified, the plate is turned over, the plate is placed in an incubator at 36 +/-1 ℃ for culturing for 48 +/-2 hours, the number of the bacterial colonies in the plate is taken out and calculated, and the bacterial colonies are multiplied by the dilution times, so that the total number of the bacterial colonies contained in each gram (per milliliter) of sample is higher than 30 percent of the total number of the bacterial colonies obtained by the common preparation process, and more bacterial colonies can be produced in the whole preparation process.
2. The GANDEW-MIX COD removing bacteria are a compound bacterial system consisting of 50 kinds of bacteria of 6 genera, can adapt to different kitchen waste environments, and mainly comprise nitrobacteria, denitrifying bacteria, bacillus, pseudomonas, activating enzyme, polysaccharide and other nutrients; the actinomycetes are used for removing the odor in the kitchen waste; the GANDEW-LIPA grease decomposition bacteria are used for removing grease in the kitchen waste, different strains are used for effectively degrading different kitchen waste, the degradable range is enlarged, and the degradation effect is more comprehensive.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments.
Example 1
A preparation process of microbial strains for degrading kitchen waste comprises the following steps of:
removing bacteria by 30% from GANDEW-MIX COD;
12% of GANDEW-LIPA lipid decomposing bacteria;
10% of actinomycetes;
30 percent of bacillus subtilis
18 percent of aspergillus niger.
Furthermore, the GANDEW-MIX COD removing bacteria are a compound bacterial system consisting of 50 kinds of bacteria of 6 genera, can adapt to different kitchen waste environments, and mainly comprise nitrobacteria, denitrifying bacteria, bacillus, pseudomonas, activating enzyme, polysaccharide and other nutrients; the actinomycetes are used for removing the odor in the kitchen waste; GANDEW-LIPA lipid decomposing bacteria are used for removing the lipid in the kitchen waste.
Further, all equipment is sterilized by steam before preparation, the technological parameters are 121-125 ℃, 0.105-0.170 MPa and 0.5-1.2 h, and equipment such as a seed tank, a fermentation tank, a dryer, a stirring mechanism and the like is needed in the preparation process.
Furthermore, the culture medium of the seeding tank is prepared from natural agricultural products such as rice, millet, corn, bran, wheat grains and the like, the culture of the mould is generally 25-28 ℃, the culture time is 4-14, and the total number of bacterial colonies is increased by 75-90%.
Furthermore, the culture medium is used for independently separating the actinomycetes, is an agar slant culture medium, contains components suitable for spores, such as bran, pea extract, peptone, some inorganic salts and the like, has a carbon source of about 1 percent and a nitrogen source of no more than 0.5 percent, has the slant culture temperature of 28-37 ℃ and the culture time of 5-14 days, and improves the total number of colonies by 65-85 percent.
Further, the fermentation of the fermentation tank is kept at 25-35 ℃ for 24-36h, and the process performs microscopic examination on the fermentation tank: the shape and density of the thallus are more than or equal to 80 percent.
Further, the stirring mechanism performs adsorption stirring after fermentation is completed, light CaCO3 or humic acid of 80 meshes is added, the feeding amount of an adsorption medium is 1-3:1, and the water content after adsorption is 25-50%.
Furthermore, the temperature of a drying heat medium of the dryer is 100-120 ℃, and the moisture of the dried product is less than 10 percent, so that the powdery microbial inoculum is formed, and the degradation efficiency is improved by 57 percent.
Furthermore, the content of the microorganism bacteria in the powdery microbial inoculum is 25-200 hundred million/g.
Further: a use method of microbial strains for degrading kitchen waste comprises the following steps:
the method comprises the following steps: when the powdery microbial inoculum is used, direct sunlight is avoided, so that the activity of bacillus in the powdery microbial inoculum, such as propagation, metabolism and the like, can be influenced and even lose activity easily caused by the direct sunlight;
step two: when in use, the water content is kept to be 60-70% because the water content suitable for the survival of the strains is kept;
step three: waiting for the microorganism to degrade, wherein the time is about 24-68 h according to different kitchen wastes and different contents.
Example 2
A preparation process of microbial strains for degrading kitchen waste comprises the following steps of:
removing bacteria by 30% from GANDEW-MIX COD;
10% of GANDEW-LIPA lipid decomposing bacteria;
8% of actinomycetes;
30 percent of bacillus subtilis
Aspergillus niger 22%.
Furthermore, the GANDEW-MIX COD removing bacteria are a compound bacterial system consisting of 50 kinds of bacteria of 6 genera, can adapt to different kitchen waste environments, and mainly comprise nitrobacteria, denitrifying bacteria, bacillus, pseudomonas, activating enzyme, polysaccharide and other nutrients; the actinomycetes are used for removing the odor in the kitchen waste; GANDEW-LIPA lipid decomposing bacteria are used for removing the lipid in the kitchen waste.
Further, all equipment is sterilized by steam before preparation, the technological parameters are 121-125 ℃, 0.105-0.170 MPa and 0.5-1.2 h, and equipment such as a seed tank, a fermentation tank, a dryer, a stirring mechanism and the like is needed in the preparation process.
Furthermore, the culture medium of the seeding tank is prepared from natural agricultural products such as rice, millet, corn, bran, wheat grains and the like, the culture of the mould is generally 25-28 ℃, the culture time is 4-14, and the total number of bacterial colonies is increased by 75-90%.
Furthermore, the culture medium is used for independently separating the actinomycetes, is an agar slant culture medium, contains components suitable for spores, such as bran, pea extract, peptone, some inorganic salts and the like, has a carbon source of about 1 percent and a nitrogen source of no more than 0.5 percent, has the slant culture temperature of 28-37 ℃ and the culture time of 5-14 days, and improves the total number of colonies by 65-85 percent.
Further, the fermentation of the fermentation tank is kept at 25-35 ℃ for 24-36h, and the process performs microscopic examination on the fermentation tank: the shape and density of the thallus are more than or equal to 80 percent.
Further, the stirring mechanism performs adsorption stirring after fermentation is completed, light CaCO3 or humic acid of 80 meshes is added, the feeding amount of an adsorption medium is 1-3:1, and the water content after adsorption is 25-50%.
Furthermore, the temperature of a drying heat medium of the dryer is 100-120 ℃, and the moisture of the dried product is less than 10 percent, so that the powdery microbial inoculum is formed, and the degradation efficiency is improved by 63 percent.
Furthermore, the content of the microorganism bacteria in the powdery microbial inoculum is 25-200 hundred million/g.
Further: a use method of microbial strains for degrading kitchen waste comprises the following steps:
the method comprises the following steps: when the powdery microbial inoculum is used, direct sunlight is avoided, so that the activity of bacillus in the powdery microbial inoculum, such as propagation, metabolism and the like, can be influenced and even lose activity easily caused by the direct sunlight;
step two: when in use, the water content is kept to be 60-70% because the water content suitable for the survival of the strains is kept;
step three: waiting for the microorganism to degrade, wherein the time is about 24-68 h according to different kitchen wastes and different contents.
Example 3
A preparation process of microbial strains for degrading kitchen waste comprises the following steps of:
removing bacteria by 30% from GANDEW-MIX COD;
8% of GANDEW-LIPA lipid decomposing bacteria;
5% of actinomycetes;
30 percent of bacillus subtilis
Aspergillus niger 27%.
Furthermore, the GANDEW-MIX COD removing bacteria are a compound bacterial system consisting of 50 kinds of bacteria of 6 genera, can adapt to different kitchen waste environments, and mainly comprise nitrobacteria, denitrifying bacteria, bacillus, pseudomonas, activating enzyme, polysaccharide and other nutrients; the actinomycetes are used for removing the odor in the kitchen waste; GANDEW-LIPA lipid decomposing bacteria are used for removing the lipid in the kitchen waste.
Further, all equipment is sterilized by steam before preparation, the technological parameters are 121-125 ℃, 0.105-0.170 MPa and 0.5-1.2 h, and equipment such as a seed tank, a fermentation tank, a dryer, a stirring mechanism and the like is needed in the preparation process.
Furthermore, the culture medium of the seeding tank is prepared from natural agricultural products such as rice, millet, corn, bran, wheat grains and the like, the culture of the mould is generally 25-28 ℃, the culture time is 4-14, and the total number of bacterial colonies is increased by 75-90%.
Furthermore, the culture medium is used for independently separating the actinomycetes, is an agar slant culture medium, contains components suitable for spores, such as bran, pea extract, peptone, some inorganic salts and the like, has a carbon source of about 1 percent and a nitrogen source of no more than 0.5 percent, has the slant culture temperature of 28-37 ℃ and the culture time of 5-14 days, and improves the total number of colonies by 65-85 percent.
Further, the fermentation of the fermentation tank is kept at 25-35 ℃ for 24-36h, and the process performs microscopic examination on the fermentation tank: the shape and density of the thallus are more than or equal to 80 percent.
Further, the stirring mechanism performs adsorption stirring after fermentation is completed, light CaCO3 or humic acid of 80 meshes is added, the feeding amount of an adsorption medium is 1-3:1, and the water content after adsorption is 25-50%.
Furthermore, the temperature of a drying heat medium of the dryer is 100-120 ℃, and the moisture of the dried product is less than 10 percent, so that the powdery microbial inoculum is formed, and the degradation efficiency is improved by 68 percent.
Furthermore, the content of the microorganism bacteria in the powdery microbial inoculum is 25-200 hundred million/g.
Further: a use method of microbial strains for degrading kitchen waste comprises the following steps:
the method comprises the following steps: when the powdery microbial inoculum is used, direct sunlight is avoided, so that the activity of bacillus in the powdery microbial inoculum, such as propagation, metabolism and the like, can be influenced and even lose activity easily caused by the direct sunlight;
step two: when in use, the water content is kept to be 60-70% because the water content suitable for the survival of the strains is kept;
step three: waiting for the microorganism to degrade, wherein the time is about 24-68 h according to different kitchen wastes and different contents.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Claims (10)
1. A preparation process of microbial strains for degrading kitchen waste is characterized by comprising the following steps of:
removing 20-30% of bacteria from GANDEW-MIX COD;
8% -15% of GANDEW-LIPA lipid decomposing bacteria;
5% -10% of actinomycetes;
15 to 35 percent of bacillus subtilis
15 to 35 percent of Aspergillus niger.
2. The preparation process of the microbial strain for degrading the kitchen waste, according to claim 1, is characterized in that: the GANDEW-MIX COD removing bacteria are a compound bacteria system consisting of 50 kinds of bacteria of 6 genera, can adapt to different kitchen waste environments, and mainly comprise nitrobacteria, denitrifying bacteria, bacillus, pseudomonas, activating enzyme, polysaccharide and other nutrients; the actinomycetes are used for removing the odor in the kitchen waste; GANDEW-LIPA lipid decomposing bacteria are used for removing the lipid in the kitchen waste.
3. The preparation process of the microbial strain for degrading the kitchen waste, according to claim 1, is characterized in that: before preparation, all equipment is subjected to steam sterilization, the process parameters are 121-125 ℃, 0.105-0.170 MPa and 0.5-1.2 h, and equipment such as a seeding tank, a fermentation tank, a dryer, a stirring mechanism and the like is required in the preparation process.
4. The preparation process of the microbial strain for degrading the kitchen waste, according to claim 3, is characterized in that: the culture medium of the seeding tank is prepared from natural agricultural products such as rice, millet, corn, bran, wheat grains and the like, the culture of mould is generally 25-28 ℃, the culture time is 4-14, and the total number of bacterial colonies is increased by 75-90%.
5. The preparation process of the microbial strain for degrading the kitchen waste, according to claim 3, is characterized in that: the culture medium is an agar slant culture medium which is used for independently separating the actinomycetes and contains components suitable for spores, such as bran, pea extract, peptone, some inorganic salts and the like, the carbon source is about 1 percent, the nitrogen source is not more than 0.5 percent, the culture temperature of the actinomycetes slant is 28-37 ℃, the culture time is 5-14 days, and the total number of colonies is increased by 75-90 percent.
6. The preparation process of the microbial strain for degrading the kitchen waste, according to claim 3, is characterized in that: the fermentation of the fermentation tank is kept at 25-35 ℃ for 24-36h, and the process is carried out on the fermentation tank by microscopic examination: the shape and density of the thallus are more than or equal to 80 percent.
7. The preparation process of the microbial strain for degrading the kitchen waste, according to claim 3, is characterized in that: the stirring mechanism is used for carrying out adsorption stirring after fermentation is completed, light CaCO3 or humic acid of 80 meshes is added, the material adding amount of an adsorption medium is 1-3:1, and the water content after adsorption is 25-50%.
8. The preparation process of the microbial strain for degrading the kitchen waste, according to claim 3, is characterized in that: the drying heat medium temperature of the dryer is 100-120 ℃, the moisture content of the dried product is less than 10%, so that the powdery microbial inoculum is formed, and the degradation efficiency is improved by 50-75%.
9. The preparation process of the microbial strain for degrading the kitchen waste, according to claim 8, is characterized in that: the content of the microbial bacteria in the powdery microbial inoculum is 25-200 hundred million/g.
10. The use method of the microbial strain for degrading the kitchen waste, according to any one of claims 1 to 9, comprises the following steps:
the method comprises the following steps: when the powdery microbial inoculum is used, direct sunlight is avoided, so that the activity of bacillus in the powdery microbial inoculum, such as propagation, metabolism and the like, can be influenced and even lose activity easily caused by the direct sunlight;
step two: when in use, the water content is kept to be 60-70% because the water content suitable for the survival of the strains is kept;
step three: waiting for the microorganism to degrade, wherein the time is about 24-68 h according to different kitchen wastes and different contents.
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