CN1119932C - Biological seed coating agent - Google Patents
Biological seed coating agent Download PDFInfo
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- CN1119932C CN1119932C CN 00109807 CN00109807A CN1119932C CN 1119932 C CN1119932 C CN 1119932C CN 00109807 CN00109807 CN 00109807 CN 00109807 A CN00109807 A CN 00109807A CN 1119932 C CN1119932 C CN 1119932C
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Abstract
The present invention relates to a biological seed coating agent which comprises carrier media, film forming agents composed of (1) polyvinyl alcohol and (2) one or more of the natural substances of starch, dextrin, chitin and cellulose or hemicellulose, at least one microorganism beneficial to plants and/or secondary metabolites. The present invention also relates to a method for preparing the biological seed coating agent and seeds coated by the biological seed coating agent.
Description
Technical field
The present invention relates to a kind of biological seed coating agent, more particularly, relate to a kind of coating agent for seed that includes the secondary metabolites after beneficial microorganism and/or its fermentation, relate to the method for preparing described biological seed coating agent.
Background technology
" " speech in this article refers to the biology of bacterium, fungi, actinomycetes and more senior or even lower level to microorganism." " speech is meant the secondary metabolite that produces behind the microbial fermentation to secondary metabolites, and these products have some special activity, as anti-disease activity and insecticidal activity.The representative of this class material has Avermectin and some antibiotic such as jinggangmycin, valida, polyoxin, chloramphenicol, streptomycin, midolthromycin, antimycin A and griseofulvin etc.
Known when legume Gen Jienei has rhizobium (Rhizobium), the nitrogen of legume in can fixed air.Rhizobium have many different kinds and bacterial strain.All there is specific useful bacterial strain in each crop, makes the different deposits yields root nodules of doing surely but differ.Contain neutral root system population in many soil, but wild strain is normally invalid.Successfully produce root nodule and depend on the existence of the effective root system bacterial strain in soil, and these bacterial strains need can be effectively and the soil environment competition, make it successfully to compete wild stocks.Guarantee that the unique method that the root knot of the effective strains of rhizobium and crop is closed is at seeding time with it and seed or soil inoculation.
Equally, also having some other microorganism can be that the growth of plant provides and improves function in many modes, improving N or the P state of plant, or prevents and treats the damage by disease and insect that some influences plant.These biologies comprise the bacterium that for example descends dependent of dead military hero: pseudomonad (Pseudomonas), Serratieae (Serratia), bacillus (Bacillus), pasteurellaceae bud bacterium (Pasteuria), azotobacter (Azotobacter), arthrobacterium (Arthrobacter), enterobacteria (Enterobacter), azospirillum (Azospirilum) and blue bacterium (Cyanobacteria), the fungi of following dependent of dead military hero: glue branch bacterium (Gliocladium), Trichoderma (Trichoderma), shield shell mould (Coniotherium), verticillium sp (Verticilium), paecilomycerol (Paecilomycea), green muscardine fungus (Metarhizium) and root nodule fungi.
These microorganisms or secondary metabolite are by using the inoculum composition for example in seed dressing or the seed coating medicine introduced plant.Though that microorganism mainly relates in this article is rhizobium (Rhizobium), should be understood that similar principle is suitable for using other microorganism above-mentioned equally.Equally, be Avermectin and jinggangmycin though secondary metabolite relates generally in this article, should be understood that similar principle is suitable for using other secondary metabolite equally.
The microorganism that is intended to use is normally by using inoculated composition such as seed dressing or seed coating medicine to introduce in the soil at seeding time.Inoculum generally makes seed closely contact with inoculation by dry, moistening or slurry inoculation technique.When slurry was inoculated, inoculum mixed with water, and used a kind of adhesive for example Arab or methylcellulose usually, and is bonding to improve.
GB 2 080 669 mentions and use water-soluble polyethylene pyrrolidones (PVP) in rhizobium inoculants.Though in this patent, mention the water-soluble polyethylene pyrrolidones the bonding of inoculum composition and leguminous seeds made moderate progress, but poor effect, and the actual survival condition of microorganism but is not as described so desirable, particularly after storing the regular hour.
On this basis, EP253673, EP306343 and EP378000 improve, and the inoculum that comprises following technical characterictic composition and method of making the same is provided: mounting medium, at least a to the microbes beneficial kind with as (1) vinylpyrrolidone of adhesive and the copolymer of the styrene (for example methyl styrene) of (2) vinyl acetate or styrene or replacement.These inoculum compositions are by for example using the necessarily vinylpyrrolidone of proportioning and the copolymer of vinyl acetate, reduce the water-soluble of copolymer, thereby increase the cohesive of inoculum composition and various leguminous seeds, increase the time-to-live of rhizobium simultaneously.But the bondability of described inoculum composition and seed is not entirely satisfactory, and also has the imperfection part as coating agent for seed.
Come in and go out and be unexpectedly, we find, one or more combination in adopting (1) polyvinyl alcohol and (2) natural materials such as starch, dextrin, chitin, cellulose and hemicellulose as film forming agent (promptly, adhesive) time, seed can more closely be combined with seed coating medicine, be difficult for peeling off between seed and the seed coating medicine, and keep seed and beneficial microorganism survival rate for a long time, and can also obviously increase the competition activity of beneficial microbe in soil environment in actual applications, thereby increase the output of crop.Finished the present invention thus.
Summary of the invention
The invention provides a kind of biological seed coating agent, it comprise as mounting medium, as (1) polyvinyl alcohol of film forming agent and (2) natural materials starch, dextrin, chitin, cellulose or hemicellulose one or more and at least a to microbes beneficial and/or secondary metabolite.Biological seed seed coating medicine of the present invention can also comprise for example suspending agent (as xanthans, carboxymethyl cellulose) etc. of other processing aids, preservative (as Sodium Benzoate, potassium sorbate etc.), antifreeze (as ethylene glycol, glycerine, urea etc.), colouring agent (, crystal violet rose-red, tartrazines, magenta etc.) as alkalescence.
The present invention also provides a kind of method for preparing biological seed coating agent, and it comprises following steps:
(a) in reactor, one or more of (1) polyvinyl alcohol and (2) natural materials such as starch, dextrin, chitin, cellulose or hemicellulose mixed with mounting medium, prepare film forming agent;
(b) with film forming agent and beneficial microbe and/or its secondary metabolite mechanical mixture in modulation kettle, prepare biological seed coating agent of the present invention.
Optional is, if desired, can also be in step (a), other processing aids such as suspending agent, preservative, antifreeze, colouring agent etc. are added with film forming agent.
The present invention also provides the seed product of the dressing of improvement, and this product comprises at least one the seed that is coated with by biological seed coating agent bag of the present invention.
The present invention also provides a kind of method for preparing the seed product of dressing, and this method comprises the water slurry system slurry of seed with biological seed coating agent of the present invention, and air-dry under the temperature that the product of gained at room temperature (that is, is not more than 30 ℃).
Film forming agent of the present invention is selected from (1) polyvinyl alcohol and (2) one or more natural products such as starch, dextrin, chitin, cellulose and hemicellulose.Particularly be preferably selected from (1) polyvinyl alcohol and (2) starch and/or dextrin, (1) polyvinyl alcohol and (2) starch, dextrin and/or chitin.Particularly preferably be (1) polyvinyl alcohol and (2) starch, dextrin and chitin, the weight ratio between polyvinyl alcohol and the natural materials is generally 1: 1-10 is preferably 1: 1-5.
Mounting medium is liquid medium such as water or organic solvent such as alcohols (for example methyl alcohol, ethanol), ketone (for example acetone, cyclohexanone), amine (for example acetamide, dimethyl formamide).Also can use solid carrier, such as synthetic material and chemical fertilizer such as ammonium sulfate, ammonium phosphate, ammonium nitrate, urea and plant resource product such as flour, bark powder, wood powder and shuck powder, cellulose powder and other solid carrier of ore soil as silica gel, silica, silicate, talcum, kaolin, lime stone, lime, chalk, bole, loess, potter's clay, dolomite, diatomite, calcium sulphate, magnesium sulfate, magnesia, grinding.Mounting medium is water preferably.
Beneficial microbe of the present invention is preferably selected from: rhizobium (Rhizobium), pseudomonad (Pseudomonas), Serratieae (Serratia), bacillus (Bacillus), pasteurellaceae bud bacterium (Pasteuria), azotobacter (Azotobacter), arthrobacterium (Arthrobacter), enterobacteria (Enterobacter), azospirillum (Azospirilum), blue bacterium (Cyanobacteria), glue branch bacterium (Gliocladium), Trichoderma (Trichoderma), shield shell mould (Coniotherium), verticillium sp (Verticilium), paecilomycerol (Paecilomycea), green muscardine fungus (Metarhizium), with the root nodule fungi, and the entomophila nematode.Rhizobium (Rhizobium) particularly.
Beneficial microbe among the present invention adopts conventional method preparation, that is, and and bacterial classification → inclined-plane → shake bottle → seeding tank → fermentation tank → concentrated → purifying.Beneficial microorganism concentration is 10 in the beneficial microbe prepared product that adopts among the present invention
11-10
13Individual bacterium/ml.
The preferred Avermectin of secondary metabolite, jinggangmycin, valida, polyoxin, chloramphenicol, streptomycin, midolthromycin, antimycin A and griseofulvin, particularly Avermectin, jinggangmycin and valida.
Secondary metabolite adopts conventional method preparation, that is, and and bacterial classification → inclined-plane → shake bottle → seeding tank → fermentation tank → knockout drum → column chromatography → concentrated.The concentration of the secondary metabolites that adopts among the present invention depends on the kind of used secondary metabolites, extermination of disease and insect pest object and the control efficiency that is intended to reach.
In biological seed coating agent of the present invention, the by weight/volume of film forming agent (or film forming agent and processing aids) and beneficial microbe (and/or secondary metabolite) is 1: 1-20, preferred 1: 1.5-10, particularly 1: 2-5.
When adopting the antibiotics secondary metabolite, should consider that the antibiotics secondary metabolite does not produce illeffects to beneficial microorganism, perhaps adopts the bacterial strain that antibiosis is have resistance.
In biological seed coating agent, in the total amount of biological seed coating agent, the content of film forming agent is generally 1-5% by weight, is preferably 2-4%, preferred especially 2.5-3.5%; The content of microorganism and/or secondary metabolite is 5-20% by weight, is preferably 10-20%, is preferably 10-15% especially; The content of processing aids is 0-20%, is preferably 1-10%.Surplus is a mounting medium.
In addition, in biological seed coating agent of the present invention, can also use chemical fungicide and insecticide.Should will consider their influences to beneficial organism equally when adopting chemical fungicide, its principle is same as above.Described fungicide is preferably selected from metalaxyl, carboxin and tmtd.Preferred insecticide is carbamate chemicals for agriculture such as carbofuran, Aldicarb, carbosulfan, Imidacloprid and Avermectin etc.
Can be by seed be mixed with biological seed coating agent of the present invention according to the seed product of dressing of the present invention, and the surface of the seed drying of the dressing of gained prepared.The type that depends on seed, the ratio of biological seed coating agent and seed can be selected from 0.5 to 2.5% the scope that accounts for the seed gross weight.
Specific embodiments
The present invention illustrates by following preparation embodiment and experimental example, but the present invention is not limited to this.
Preparation embodiment 1
In 1500 liters of reactors, 20 kilograms of polyvinyl alcohol and 20 kilograms of starch, 8 kilograms of dextrin are stirred in 850 kg of water, prepare the film forming agent aqueous suspensions.Film forming agent aqueous suspensions and 200 liters of rhizobium of bean pure cultures of gained (are on average contained 10
12The mechanical mixture in 1500 liters of modulation kettles of individual bacterium/mL) is prepared biological seed coating agent of the present invention.
Then 40 parts of weight soybean are mixed with 1 part of weight the invention described above biological seed coating agent, and at room temperature air-dry, prepare the soya seeds of biological seed coating agent dressing.
Preparation embodiment 2
In 1500 liters of reactors, 20 kilograms of polyvinyl alcohol and 5 kilograms of chitins, 1.5 kilograms of xanthans are stirred in 850 kg of water, prepare the film forming agent aqueous suspensions.Film forming agent aqueous suspensions and 200 liters of rhizobium of bean pure cultures of gained (are on average contained 10
12The mechanical mixture in 1500 liters of modulation kettles of individual bacterium/mL) is prepared biological seed coating agent of the present invention.
Then 40 gram soya seeds are stated biological seed coating agent of the present invention with 10 grams and mix, and at room temperature air-dry, prepare soya seeds by the biological seed coating agent dressing.
Preparation embodiment 3
In 1500 liters of reactors, 20 kilograms of polyvinyl alcohol and 5 kilograms of chitins, 3 kilograms of xanthans, 5 kilograms of carboxymethyl celluloses are stirred in 882kg water, prepare the film forming agent aqueous suspensions.Film forming agent aqueous suspensions and 200 liters of living soon rhizobium pure cultures of soybean of gained (are all contained 10
12The mechanical mixture in 1500 liters of modulation kettles of individual bacterium/mL) is prepared biological seed coating agent of the present invention.
Then 10 parts of weight soya seeds are mixed with 1 part of weight the invention described above biological seed coating agent, and at room temperature air-dry, prepare soya seeds by the biological seed coating agent dressing.
Preparation embodiment 4
In 1500 liters of reactors, with 20 kilograms of polyvinyl alcohol and 20kg starch, 10 kilograms of dextrin, 2 kilograms of xanthans, 100 kilograms of ethylene glycol, 1 kilogram of Sodium Benzoate, 1 kilogram of alkalescence is rose-red stirs in 200 kg of water, prepares the film forming agent aqueous suspensions.The film forming agent aqueous suspensions and black rhizobium (R.Leguminosarum) pure culture of pea of gained (are on average contained 10
12The mechanical mixture in 1500 liters of modulation kettles of individual bacterium/mL) is prepared biological seed coating agent of the present invention.
Then 40 parts of weight pea seeds are mixed with the kind clothing agent of 1 part of weight the invention described above biology, and at room temperature under the wind, prepare pea seed by the biological seed coating agent dressing.
Preparation embodiment 5
In 1500 liters of reactors, with 20 kilograms of polyvinyl alcohol and 10 kilograms of dextrin, 1 kilogram of xanthans, 1 kilogram of carboxymethyl cellulose, 100 kilograms of ethylene glycol, 1 kilogram of Sodium Benzoate, 1 kilogram of alkaline rose is existing red stirs in 200 kg of water, prepares the film forming agent aqueous suspensions.The film forming agent aqueous suspensions and rhizobium phaseoli (Rhizobium meliloti) pure culture of gained (are on average contained 10
12Individual bacterium/, prepare biological seed coating agent of the present invention mL) 1500 liters of modulation kettle still mechanical mixture.
Then 40 parts of weight phaseolus vulgaris seeds are mixed with the kind clothing agent of 1 kilogram of the invention described above biology, and at room temperature air-dry, prepare phaseolus vulgaris seeds by the biological seed coating agent dressing.
Preparation embodiment 6
At 1500 liters of reactors, 20 kilograms of polyvinyl alcohol and 10 kilograms of dextrin, 1 kilogram of xanthans, 80 kilograms of ethylene glycol, 1 kilogram of alkalescence is rose-red, 100 kilograms of tmtd stir in 900 kg of water, prepare the film forming agent aqueous suspensions.The film forming agent aqueous suspensions of gained and 0.8 kilogram of Avermectin and 200 liters of peanut rhizobium pure cultures (are on average contained 10
12The mechanical mixture in 1500 liters of modulation kettles of individual bacterium/mL) is prepared biological seed coating agent of the present invention.
Then 40 parts of weight peanut seeds are mixed with the kind clothing agent of 1 part of weight the invention described above biology, and at room temperature air-dry, prepare soya seeds by the biological seed coating agent dressing.
Preparation embodiment 7
In 1500 liters of reactors, 20 kilograms of polyvinyl alcohol and 10 kilograms of dextrin, 0.8 kilogram of xanthans, 100 kilograms of ethylene glycol, 1 kilogram of rose-red 50 kilograms of diatomite through sand milling of alkalescence are stirred in 850 kg of water, prepare the film forming agent aqueous suspensions.With the film forming agent aqueous suspensions of gained and 6 kilograms of jinggangmycins, (on average contain 10 through 15kg Imidacloprid and 200 liters of rhizobium of bean pure cultures of sand milling
12The mechanical mixture in 1500 liters of modulation kettles of individual bacterium/mL) is prepared biological seed coating agent of the present invention.
Then 40 parts of weight soya seeds are mixed with the kind clothing agent of 1 part of weight the invention described above biology, and at room temperature air-dry, prepare by the soya seeds of biological species at the agent dressing.
Preparation embodiment 8
In 1500 liters of reactors, 20 kilograms of polyvinyl alcohol are mixed with 10 kilograms of dextrin, 1.5 kilograms of g xanthans, 90 kilograms of ethylene glycol, 1 kilogram of alkalescence 15kg Imidacloprid, 6 kilograms of jinggangmeisus rose-red, the process sand milling, prepare the film forming agent water slurry.Again with 200 kilograms of bacillus subtilises (Eco201,10
12/ mL) the kind clothing agent that is used for wheat is promptly prepared in mixing.
35 parts of weight wheat seeds and the agent of 1 part of weight kind clothing are mixed, and at room temperature air-dry, prepare wheat seed coating agent by the biological seed coating agent dressing.
Preparation embodiment 9
In 1500 liters of reactors, with 20 kilograms of polyvinyl alcohol and 10 kilograms of dextrin, 1.5 kilograms of xanthans, 90 kilograms of ethylene glycol, 1 kilogram of rose-red, 100 kilograms of carbofuran through sand millings of alkalescence, 30 kilograms of precipitated calcium carbonates through sand millings are prepared film forming agent suspension and 100 liters of azospirillums (10
12/ mL viable bacteria) and 100 liters of silicate bacteriums (potassium decomposing bacteria, 10
12/ mL) mixing promptly gets indication biological seed coating agent of the present invention.
30 parts of weight corn seeds and above-mentioned kind of clothing agent of 1 part of weight are mixed, promptly get the corn coating seed.
In order to investigate the field performance of the seed of handling with biological seed coating agent, under the environmental condition of land for growing field crops, done district's group small plot experiment at random, prior to seeding, measuring was carried out in the level of N, P and three kinds of main nutrient elements of K in each experimental plot, showed that each minizone do not have significant difference.
Experimental example 1
The present invention is planted clothing agent and soya seeds by 1: behind the proportioning dressing of 40w/w, contrast is with clear water seed dressing, sowing in the usual way.Emerged back 40 days, and claimed individual plant and average knurl heavy fixed nitrogen.The acetylene reducing process is adopted in determination of activity.Nodule nitrogen fixation is measured than living behind the table 1 biological seed coating agent dressing
| Sample number | Average knurl heavy (mg) | Fixed nitrogen is than nmolC alive 2H 4，min -1mg -1 |
| Preparation embodiment 1 | ?9 | ?568.9 |
| Preparation embodiment 1 | ?9 | ?470.4 |
| Preparation embodiment 1 | ?8.6 | ?512.8 |
| Contrast | ?8.3 | ?443.9 |
| Standard specimen | Ethene | ?0 |
Result from table 1 as seen, soya seeds is with after the biological seed coating agent seed coating medicine Cotton seeds of the present invention, the root nodule specific activity not activity of Cotton seeds has tangible increase.
Experimental example 2
In order to investigate biological seed coating agent leguminous plant is connect the influence of knurl amount and dross speed, carried out the dross token test with soybean.The results are shown in Table 2.
Different disposal plant dross token test behind the table 2 biological seed coating agent dressing
| Handle | Initial day of dross | Inoculating after 50 days the strain knurl looses | Inoculate individual plant knurl weight after 50 days |
| Preparation embodiment 1 | ?6 | ?76±21 | ?0.29 |
| Preparation embodiment 2 | ?6 | ?45±10 | ?0.23 |
| Preparation embodiment 3 | ?7 | ?20±6 | ?0.17 |
| Contrast | ?8 | ?34±9 | ?0.15 |
Result from table 2 as seen, soya seeds is with after the biological seed coating agent seed coating medicine Cotton seeds of the present invention, plant dross time and dross are to be improved.
Experimental example 3
In order to investigate the preventive effect of biological seed coating agent capsuled seed to underground insect, adopt the kind clothing agent of preparation embodiment 8, be subjects with the corn, after wheat is emerged back 20, the preventive effect of wireworm is investigated with the sieve local method.The results are shown in Table 3.The wheat seed of table 3 Cotton seeds is to the investigation of wireworm preventive effect
| Sample number | Wireworm is (individual/M 2) | Hazard ratio (%) |
| Handle 1 | ?21 | ?7 |
| Handle 2 | ?18 | ?8 |
| Handle 3 | ?32 | ?11 |
| Contrast | ?47 | ?15 |
As can be seen from Table 3, carry out Cotton seeds with the kind clothing agent that contains insecticide after, obviously suppressed the harm of wireworm.
Experimental example 4
To contain the control efficiency of biological antibiotic biological seed coating agent in order investigating, to adopt the kind clothing agent of preparation embodiment 9 to carry out wheat sharp eyespot control test crop pest.The results are shown in Table 4.
The wheat seed of table 4 Cotton seeds is to the investigation of wheat sharp eyespot preventive effect
| Sample number | Disease index | Preventive effect (%) |
| Handle 1 | ?6.05 | ?67 |
| Handle 2 | ?6.08 | ?59 |
| Handle 3 | ?8.12 | ?57 |
| Contrast | ?20.17 | ?0 |
Experimental example 5
In order to investigate the final influence of biological seed coating agent, the soybean of small plot experiment has been carried out determination of yield to crop yield.The results are shown in Table 5.Soybean yields test behind the table 5 biological seed coating agent dressing
| Handle | Actual measurement output (kg/ha) | Volume increase (%) |
| Preparation embodiment 1 | ?2984 | ?17 |
| Preparation embodiment 2 | ?2882 | ?13 |
| Preparation embodiment 3 | ?3085 | ?21 |
| Contrast | ?2550 | ?0 |
Result from table 5 as seen, soya seeds is with after the biological seed coating agent seed coating medicine Cotton seeds of the present invention, the output of soybean has tangible increase.
Though the present invention has done detailed description in conjunction with specific embodiments and embodiment, should be appreciated that in the scope that does not depart from spirit of the present invention many variation scheme and improvement project can be arranged.These schemes all within the scope of the present invention.
Claims (8)
1. biological seed coating agent, it comprises as mounting medium, (1) polyvinyl alcohol of film forming agent is selected from starch with (2) natural materials, dextrin, chitin, one or more of cellulose or hemicellulose, with at least a microbes beneficial is selected from rhizobium (Rhizobium), pseudomonad (Pseudomonas), Serratieae (Serratia), bacillus (Bacillus), pasteurellaceae bud bacterium (Pasteuria), azotobacter (Azotobacter), arthrobacterium (Arthrobacter), enterobacteria (Enterobacter), azospirillum (Azospirilum) and blue bacterium (Cyanobacteria), the fungi of following dependent of dead military hero: glue branch bacterium (Gliocladium), Trichoderma (Trichoderma), shield shell mould (Coniotherium), verticillium sp (Verticilium), paecilomycerol (Paecilomycea), green muscardine fungus (Metarhizium), or secondary metabolite is selected from Avermectin, jinggangmeisu and processing aids, the weight ratio of polyvinyl alcohol and natural materials is 1 in the described film forming agent: 1-10, wherein, the content of film forming agent is the 1-5% of biological seed coating agent by weight, to microbes beneficial or secondary metabolite by volume/weight counts the 5-20% of biological seed coating agent, processing aids is the 0-20% of biological seed coating agent by weight, and surplus is a mounting medium.
2. according to the biological seed coating agent of claim 1, wherein film forming agent and described beneficial microorganism weight with the ratio of volume are: 1: 1-20.
3. according to the biological seed coating agent of claim 1, wherein beneficial microbe is rhizobium.
4. according to the biological seed coating agent of one of claim 1-3, described processing aids is one or more of suspending agent, preservative, antifreeze, colouring agent.
5, according to the biological seed coating agent of claim 4, wherein suspending agent is selected from xanthans and/or carboxymethyl cellulose.
6, according to the biological seed coating agent of claim 4, wherein mounting medium can be selected from synthetic material, plant resource product of water, alcohols, ketone, amide-type, ore soil, grinding and composition thereof.
7, a kind of method for preparing biological seed coating agent, wherein biological seed coating agent comprises as mounting medium, (1) polyvinyl alcohol of film forming agent is selected from starch with (2) natural materials, dextrin, chitin, one or more of cellulose or hemicellulose, with at least a microbes beneficial is selected from rhizobium (Rhizobium), pseudomonad (Pseudomonas), Serratieae (Serratia), bacillus (Bacillus), pasteurellaceae bud bacterium (Pasteuria), azotobacter (Azotobacter), arthrobacterium (Arthrobacter), enterobacteria (Enterobacter), azospirillum (Azospirilum) and blue bacterium (Cyanobacteria), the fungi of following dependent of dead military hero: glue branch bacterium (Gliocladium), Trichoderma (Trichoderma), shield shell mould (Coniotherium), verticillium sp (Verticilium), paecilomycerol (Paecilomycea), green muscardine fungus (Metarhizium), or secondary metabolite is selected from Avermectin, jinggangmeisu and processing aids, the weight ratio of polyvinyl alcohol and natural materials is 1 in the described film forming agent: 1-10, wherein, the content of film forming agent is the 1-5% of biological seed coating agent by weight, to microbes beneficial or secondary metabolite by volume/weight counts the 5-20% of biological seed coating agent, processing aids is the 0-20% of biological seed coating agent by weight, surplus is a mounting medium, comprises following steps:
(a) in reactor, one or more that (1) polyvinyl alcohol and (2) natural materials are selected from starch, dextrin, chitin, cellulose or hemicellulose mixes with mounting medium, prepares film forming agent;
(b) with film forming agent and beneficial microbe or its secondary metabolite mechanical mixture in modulation kettle.
8, according to the method for claim 7, wherein also be included in the step (a), with processing aids: suspending agent, preservative, antifreeze, colouring agent mix with film forming agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00109807 CN1119932C (en) | 2000-07-07 | 2000-07-07 | Biological seed coating agent |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00109807 CN1119932C (en) | 2000-07-07 | 2000-07-07 | Biological seed coating agent |
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| CN1276973A CN1276973A (en) | 2000-12-20 |
| CN1119932C true CN1119932C (en) | 2003-09-03 |
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| CN 00109807 Expired - Fee Related CN1119932C (en) | 2000-07-07 | 2000-07-07 | Biological seed coating agent |
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Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE0300801D0 (en) * | 2003-03-21 | 2003-03-21 | Paul Gatenholm | Polymeric film or coating comprising hemicellulose |
| CN100340498C (en) * | 2004-09-11 | 2007-10-03 | 中国石油化工股份有限公司 | Microbial filler and production thereof |
| US9102893B2 (en) | 2005-09-08 | 2015-08-11 | Advanced Biological Marketing | Equipment lubricating microbial compositions |
| EP1928995A4 (en) * | 2005-09-08 | 2009-11-25 | Cornell Res Foundation Inc | Formulations of viable microorganisms and their methods of production and use |
| CN101993295A (en) * | 2009-08-31 | 2011-03-30 | 夏荣堂 | Microbial soaking seed coating of rice |
| CN102070368B (en) * | 2010-08-23 | 2012-05-02 | 中国农业大学 | Fulvic acid and rhizobium biological type leguminous plant seed coating agent and preparation method thereof |
| CN101999415B (en) * | 2010-09-29 | 2013-06-12 | 中国海洋大学 | Method for preparing bioactive polysaccharide seed coating agent |
| CN101967461B (en) * | 2010-10-22 | 2012-06-27 | 领先生物农业股份有限公司 | Fermentation method for azospirillum, fermentation liquor prepared by method and application of fermentation liquor |
| CN102659471B (en) * | 2012-04-24 | 2014-05-21 | 上海师范大学 | Mycelium drought-resistant seed coating and preparation method thereof |
| CN104016815B (en) * | 2014-06-20 | 2018-03-20 | 山东省花生研究所 | A kind of peanut seed coating agent |
| CN104542716A (en) * | 2014-12-30 | 2015-04-29 | 天津市工业微生物研究所有限公司 | Novel biological seed coating agent and preparation method thereof |
| CN109220066A (en) * | 2017-11-28 | 2019-01-18 | 卢梅雅 | Azotica is improving the application in sweet potato potato seed emergence quality |
| CN109566002A (en) * | 2018-11-29 | 2019-04-05 | 冯书妙 | A kind of microorganism seed coating method |
| CN109757488B (en) * | 2019-02-20 | 2022-03-04 | 广东省农业科学院植物保护研究所 | Microbial seed pelleting coating composition |
| CN110946136B (en) * | 2019-12-16 | 2022-02-18 | 山东省农作物种质资源中心 | Wheat seed coating agent and preparation method thereof |
| CN113016826B (en) * | 2021-04-19 | 2022-07-08 | 北京中农富源集团有限公司 | Preparation process of multifunctional microbial organic seed coating agent |
| CN114436702A (en) * | 2022-03-11 | 2022-05-06 | 陕西科技大学 | Degradable mulberry seed coating film agent and preparation method and use method thereof |
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2000
- 2000-07-07 CN CN 00109807 patent/CN1119932C/en not_active Expired - Fee Related
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