CN111991346A - Gel dressing for preventing and treating human papilloma virus infection and preparation method thereof - Google Patents
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Abstract
The invention discloses a gel dressing for preventing and treating human papilloma virus infection, which is prepared from CP protein, carbomer, carboxylated chitosan, glycerol, Arabic gum, liquorice and saffron crocus in percentage by mass as follows: CP protein: carbomer: carboxylated chitosan: glycerol: gum arabic: licorice root: saffron = 0.15-0.25: 2-3: 3-4: 4-5: 0.1-1: 0.1-0.5; the balance of water; the gel dressing is prepared from the following raw materials in parts by mass: CP protein: carbomer: carboxylated chitosan: glycerol: gum arabic: licorice root: saffron =0.15:2:2:3:4:0.1: 0.1; the balance of water; the gel dressing is prepared from the following raw materials in parts by mass: CP protein: carbomer: carboxylated chitosan: glycerol: gum arabic: licorice root: saffron =0.25:3:3:4:5:1: 0.5; the balance of water; according to the invention, the gel dressing is applied to the preparation of the medicine for preventing and treating human papilloma virus infection, and meanwhile, the affinity and the functionality of the original gel dressing are improved.
Description
Technical Field
The invention relates to the technical field of medical treatment, in particular to a gel dressing for preventing and treating human papilloma virus infection and a preparation method thereof.
Background
In the 20 th century, 80 th german virologist haraldzur hausen first suggested that HPV infection is closely related to cervical cancer, and in 1995, the conference on IRAC (international cancer research organization) established that HPV infection is a necessary condition for cervical cancer development, HPV could be detected in 99.7% of cervical cancer tissues, and cervical cancer hardly occurred in uninfected HPV patients (negative predictive value > 99%). As the etiology of cervical cancer is revealed, Hausen has become one of the leaders of the 2008 nobel medical prize.
The high-risk HPV is a virus with minimum double-stranded DNA coated by protein, more than 100 types of HPV are typed at present, the HPV is a mucotropic and skin epithelial virus which has high host and specificity, transient infection is often caused by sexual contact, the transient infection enters a body from tiny wounds on an epithelial basal layer, and the transient infection is propagated in tissue cells in a DNA replication mode to cause proliferative lesions with different degrees.
High risk type human papilloma virus: the types of HPV16, 18, 33 and the like are related to the generation of malignant tumors such as cervical cancer, cervical epithelial sarcoma and the like, so the HPV is called high-risk type HPV, and can cause more than 79% of cervical cancer and more than 50% of cervical epithelial neoplasia (CINII-III). Sub-high risk human papillomavirus: the types including HPV31, 39, 45, 51, 52, 53, 56, 59, 66, 68, 73, 82, 83 and the like belong to high-risk subtypes. Low risk human papillomavirus: the HPV6, 11, 42, 43 and 81 can cause benign lesions such as condyloma acuminatum and laryngeal papilloma, are the most common pathogenic types, and are low-risk types.
HPV infection is one of the main causes of cervical cancer, and 99.7 percent of cervical cancer can find high-risk type HPV infection. Persistent infection with high-risk HPV increases the risk of cervical cancer by 250-fold, and CINIII (severe dysplasia and carcinoma in situ) develops in the cervical epithelium only if HPV is persistently infected, and CINIII or carcinoma develops in about 40% of women with low-grade cervical lesions and remains at this level. Therefore, high-risk type HPV infection is a reliable index for predicting cervical lesion deterioration.
Human papillomavirus infection can be prevented and treated by vaccines, but the vaccines have high price, inconvenient storage and a plurality of use limitations, and in addition, the vaccines can be prevented and treated by external application medicines, the medicines on the market are fewer at present, and the patient selectivity is low; meanwhile, some existing dressings for treating human papilloma virus infection are not compatible with skin and have low functionality; in view of these drawbacks, there is a need for a gel dressing for preventing and treating human papillomavirus infection and a preparation method thereof.
Disclosure of Invention
The invention aims to provide a gel dressing for preventing and treating human papillomavirus infection and a preparation method thereof, and the application of the gel dressing in preparing a medicament for preventing and treating human papillomavirus infection can improve the affinity and functionality of the original gel dressing.
The purpose of the invention can be realized by the following technical scheme:
a gel dressing for preventing and treating human papilloma virus infection is prepared from CP protein, carbomer, carboxylated chitosan, glycerol, acacia, Glycyrrhrizae radix and stigma croci Sativi, wherein the mass percentages of the raw materials are as follows: CP protein: carbomer: carboxylated chitosan: glycerol: gum arabic: licorice root: saffron = 0.15-0.25: 2-3: 3-4: 4-5: 0.1-1: 0.1-0.5; the balance being water.
As a further scheme of the invention: the gel dressing is prepared from the following raw materials in parts by mass: CP protein: carbomer: carboxylated chitosan: glycerol: gum arabic: licorice root: saffron =0.15:2:2:3:4:0.1: 0.1; the balance being water.
As a further scheme of the invention: the gel dressing is prepared from the following raw materials in parts by mass: CP protein: carbomer: carboxylated chitosan: glycerol: gum arabic: licorice root: saffron =0.25:3:3:4:5:1: 0.5; the balance being water.
As a further scheme of the invention: the gel dressing is prepared from the following raw materials in parts by mass: CP protein: carbomer: carboxylated chitosan: glycerol: gum arabic: licorice root: saffron =0.2:2.5:2.5:3.5:4.5:0.5: 0.25; the balance being water.
As a further scheme of the invention: the preparation method of the gel dressing for preventing and treating human papilloma virus infection comprises the following steps:
s1, weighing the raw materials according to the formula;
s2, adding carbomer into water, swelling to obtain carbomer aqueous solution, respectively preparing CP protein and acacia into aqueous solution, grinding radix Glycyrrhizae and stigma croci Sativi to obtain radix Glycyrrhizae and stigma croci Sativi mixed aqueous solution, dissolving carboxylated chitosan in water, mixing with carbomer aqueous solution to obtain mucilage, sequentially adding acacia aqueous solution, glycerol, radix Glycyrrhizae and stigma croci Sativi mixed aqueous solution into mucilage, mixing, slowly adding CP protein solution into mucilage, adding water to full dose, and mixing to obtain the final product.
As a further scheme of the invention: the gel dressing is applied to the preparation of medicines for preventing and treating human papilloma virus infection.
The invention has the beneficial effects that: the CP protein is negatively charged as a whole and can be complexed with positive charges on the HPV protein capsid, so that the HPV protein conformation is changed and inactivated; simultaneously, carbomer and carboxylated chitosan adsorb and discharge the inactivated HPV out of the body; CP protein and carbomer accelerate the rapid recovery and healing of damaged and eroded tissues. Thereby achieving the purpose of preventing and treating HPV infection and having the function of preventing the generation of cervical cancer; the liquorice can change the taste, and can be used for conditioning gel dressing, so that the liquorice can not irritate the nose and is more suitable for patients; the saffron has the effects of dredging the channels and activating collaterals, clearing and activating channels and collaterals, dissipating blood stasis and resolving masses, relieving swelling and pain, cooling blood and detoxifying and the like, and particularly for people who are often in depression, people can feel smooth after long-term insisting on taking the saffron, the immunity of the human body is comprehensively improved, and the gel dressing is more skin-friendly.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A gel dressing for preventing and treating human papilloma virus infection is prepared from CP protein, carbomer, carboxylated chitosan, glycerol, acacia, Glycyrrhrizae radix and stigma croci Sativi, and the mass fraction ratio of the raw materials is as follows: CP protein: carbomer: carboxylated chitosan: glycerol: gum arabic: licorice root: saffron =0.15:2:2:3:4:0.1: 0.1; the balance of water;
the preparation method of the gel dressing for preventing and treating human papilloma virus infection comprises the following steps:
s1, weighing the raw materials according to the formula;
s2, adding carbomer into water, swelling to obtain carbomer aqueous solution, respectively preparing CP protein and acacia into aqueous solution, grinding radix Glycyrrhizae and stigma croci Sativi to obtain radix Glycyrrhizae and stigma croci Sativi mixed aqueous solution, dissolving carboxylated chitosan in water, mixing with carbomer aqueous solution to obtain mucilage, sequentially adding acacia aqueous solution, glycerol, radix Glycyrrhizae and stigma croci Sativi mixed aqueous solution into the mucilage, mixing, slowly adding CP protein solution into the mucilage, adding water to full dose, and mixing to obtain the product;
the method specifically comprises the following steps: weighing the raw materials according to the formula amount; adding carbomer into water, heating to 70-80 ℃, stirring for 20-40 minutes, and standing overnight at room temperature to fully swell carbomer; dissolving CP protein in water; adding water into Arabic gum, uniformly mixing, and carrying out water bath at 70-80 ℃ for 20-40 minutes; grinding Glycyrrhrizae radix and stigma croci Sativi to obtain Glycyrrhrizae radix and stigma croci Sativi mixed water solution, dissolving carboxylated chitosan in water, mixing with carbomer water solution to obtain mucilage, adding other substances, mixing, and adding fibroin protein in gel; sequentially adding arabic gum aqueous solution, glycerol, and mixed aqueous solution of Glycyrrhrizae radix and stigma croci Sativi into the mucilage, and mixing; slowly adding the CP protein solution into the mucilage, adding water to full amount, and mixing uniformly to obtain the final product;
the CP protein is prepared by the following method: shearing pupa Bombyx Bombycis, mixing with 0.5% sodium carbonate solution at a material-to-liquid ratio of 1:50(g/mL), boiling for 4 times, repeatedly scrubbing with distilled water, and oven drying; after drying, mixing the dried powder with 9M lithium bromide solution according to the material-liquid ratio of 1:5(g/mL), heating at 60 ℃ for 4 hours, centrifuging to remove insoluble substances, and transferring the mixture to a dialysis bag with the cut-off molecular weight of 8000-14000 for dialysis for 2 days; and (5) freeze-drying the dialyzate to obtain freeze-dried powder, namely the CP protein.
Example 2
A gel dressing for preventing and treating human papilloma virus infection is prepared from CP protein, carbomer, carboxylated chitosan, glycerol, acacia, Glycyrrhrizae radix and stigma croci Sativi, and the mass fraction ratio of the raw materials is as follows: CP protein: carbomer: carboxylated chitosan: glycerol: gum arabic: licorice root: saffron =0.25:3:3:4:5:1: 0.5; the balance of water;
the preparation method of the gel dressing for preventing and treating human papilloma virus infection comprises the following steps:
s1, weighing the raw materials according to the formula;
s2, adding carbomer into water, swelling to obtain carbomer aqueous solution, respectively preparing CP protein and acacia into aqueous solution, grinding radix Glycyrrhizae and stigma croci Sativi to obtain radix Glycyrrhizae and stigma croci Sativi mixed aqueous solution, dissolving carboxylated chitosan in water, mixing with carbomer aqueous solution to obtain mucilage, sequentially adding acacia aqueous solution, glycerol, radix Glycyrrhizae and stigma croci Sativi mixed aqueous solution into the mucilage, mixing, slowly adding CP protein solution into the mucilage, adding water to full dose, and mixing to obtain the product;
the method specifically comprises the following steps: weighing the raw materials according to the formula amount; adding carbomer into water, heating to 70-80 ℃, stirring for 20-40 minutes, and standing overnight at room temperature to fully swell carbomer; dissolving CP protein in water; adding water into Arabic gum, uniformly mixing, and carrying out water bath at 70-80 ℃ for 20-40 minutes; grinding Glycyrrhrizae radix and stigma croci Sativi to obtain Glycyrrhrizae radix and stigma croci Sativi mixed water solution, dissolving carboxylated chitosan in water, mixing with carbomer water solution to obtain mucilage, adding other substances, mixing, and adding fibroin protein in gel; sequentially adding arabic gum aqueous solution, glycerol, and mixed aqueous solution of Glycyrrhrizae radix and stigma croci Sativi into the mucilage, and mixing; slowly adding the CP protein solution into the mucilage, adding water to full amount, and mixing uniformly to obtain the final product;
the CP protein is prepared by the following method: shearing pupa Bombyx Bombycis, mixing with 0.5% sodium carbonate solution at a material-to-liquid ratio of 1:50(g/mL), boiling for 4 times, repeatedly scrubbing with distilled water, and oven drying; after drying, mixing the dried powder with 9M lithium bromide solution according to the material-liquid ratio of 1:5(g/mL), heating at 60 ℃ for 4 hours, centrifuging to remove insoluble substances, and transferring the mixture to a dialysis bag with the cut-off molecular weight of 8000-14000 for dialysis for 2 days; and (5) freeze-drying the dialyzate to obtain freeze-dried powder, namely the CP protein.
Example 3
A gel dressing for preventing and treating human papilloma virus infection is prepared from CP protein, carbomer, carboxylated chitosan, glycerol, acacia, Glycyrrhrizae radix and stigma croci Sativi, and the mass fraction ratio of the raw materials is as follows: CP protein: carbomer: carboxylated chitosan: glycerol: gum arabic: licorice root: saffron =0.2:2.5:2.5:3.5:4.5:0.5: 0.25; the balance of water;
the preparation method of the gel dressing for preventing and treating human papilloma virus infection comprises the following steps:
s1, weighing the raw materials according to the formula;
s2, adding carbomer into water, swelling to obtain carbomer aqueous solution, respectively preparing CP protein and acacia into aqueous solution, grinding radix Glycyrrhizae and stigma croci Sativi to obtain radix Glycyrrhizae and stigma croci Sativi mixed aqueous solution, dissolving carboxylated chitosan in water, mixing with carbomer aqueous solution to obtain mucilage, sequentially adding acacia aqueous solution, glycerol, radix Glycyrrhizae and stigma croci Sativi mixed aqueous solution into the mucilage, mixing, slowly adding CP protein solution into the mucilage, adding water to full dose, and mixing to obtain the product;
the method specifically comprises the following steps: weighing the raw materials according to the formula amount; adding carbomer into water, heating to 70-80 ℃, stirring for 20-40 minutes, and standing overnight at room temperature to fully swell carbomer; dissolving CP protein in water; adding water into Arabic gum, uniformly mixing, and carrying out water bath at 70-80 ℃ for 20-40 minutes; grinding Glycyrrhrizae radix and stigma croci Sativi to obtain Glycyrrhrizae radix and stigma croci Sativi mixed water solution, dissolving carboxylated chitosan in water, mixing with carbomer water solution to obtain mucilage, adding other substances, mixing, and adding fibroin protein in gel; sequentially adding arabic gum aqueous solution, glycerol, and mixed aqueous solution of Glycyrrhrizae radix and stigma croci Sativi into the mucilage, and mixing; slowly adding the CP protein solution into the mucilage, adding water to full amount, and mixing uniformly to obtain the final product;
the CP protein is prepared by the following method: shearing pupa Bombyx Bombycis, mixing with 0.5% sodium carbonate solution at a material-to-liquid ratio of 1:50(g/mL), boiling for 4 times, repeatedly scrubbing with distilled water, and oven drying; after drying, mixing the dried powder with 9M lithium bromide solution according to the material-liquid ratio of 1:5(g/mL), heating at 60 ℃ for 4 hours, centrifuging to remove insoluble substances, and transferring the mixture to a dialysis bag with the cut-off molecular weight of 8000-14000 for dialysis for 2 days; and (5) freeze-drying the dialyzate to obtain freeze-dried powder, namely the CP protein.
The experimental data steps are the same as the specific implementation steps of the patent with the application number of 202010141732.8, and the CP protein is wholly negatively charged and can be mutually complexed with positive charges on the HPV protein capsid, so that the HPV protein conformation is changed and inactivated; simultaneously, carbomer and carboxylated chitosan adsorb and discharge the inactivated HPV out of the body; CP protein and carbomer accelerate the rapid recovery and healing of damaged and eroded tissues. Thereby achieving the purpose of preventing and treating HPV infection and having the function of preventing the generation of cervical cancer; the liquorice can change the taste, and can be used for conditioning gel dressing, so that the liquorice can not irritate the nose and is more suitable for patients; the saffron has the effects of dredging the channels and activating collaterals, clearing and activating channels and collaterals, dissipating blood stasis and resolving masses, relieving swelling and pain, cooling blood and detoxifying and the like, and particularly for people who are often in depression, people can feel smooth after long-term insisting on taking the saffron, the immunity of the human body is comprehensively improved, and the gel dressing is more skin-friendly.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.
Claims (6)
1. The gel dressing for preventing and treating human papilloma virus infection is characterized in that raw materials comprise CP protein, carbomer, carboxylated chitosan, glycerol, Arabic gum, liquorice and saffron crocus, and the mass fraction ratio of the raw materials is as follows: CP protein: carbomer: carboxylated chitosan: glycerol: gum arabic: licorice root: saffron = 0.15-0.25: 2-3: 3-4: 4-5: 0.1-1: 0.1-0.5; the balance being water.
2. The gel dressing for preventing and treating human papillomavirus infection according to claim 1, wherein the gel dressing is made from the following raw materials in percentage by mass: CP protein: carbomer: carboxylated chitosan: glycerol: gum arabic: licorice root: saffron =0.15:2:2:3:4:0.1: 0.1; the balance being water.
3. The gel dressing for preventing and treating human papillomavirus infection according to claim 1, wherein the gel dressing is made from the following raw materials in percentage by mass: CP protein: carbomer: carboxylated chitosan: glycerol: gum arabic: licorice root: saffron =0.25:3:3:4:5:1: 0.5; the balance being water.
4. The gel dressing for preventing and treating human papillomavirus infection according to claim 1, wherein the gel dressing is made from the following raw materials in percentage by mass: CP protein: carbomer: carboxylated chitosan: glycerol: gum arabic: licorice root: saffron =0.2:2.5:2.5:3.5:4.5:0.5: 0.25; the balance being water.
5. The method for preparing the gel dressing for preventing and treating human papillomavirus infection according to claim 1, characterized by comprising the following steps:
s1, weighing the raw materials according to the formula;
s2, adding carbomer into water, swelling to obtain carbomer aqueous solution, respectively preparing CP protein and acacia into aqueous solution, grinding radix Glycyrrhizae and stigma croci Sativi to obtain radix Glycyrrhizae and stigma croci Sativi mixed aqueous solution, dissolving carboxylated chitosan in water, mixing with carbomer aqueous solution to obtain mucilage, sequentially adding acacia aqueous solution, glycerol, radix Glycyrrhizae and stigma croci Sativi mixed aqueous solution into mucilage, mixing, slowly adding CP protein solution into mucilage, adding water to full dose, and mixing to obtain the final product.
6. Use of the gel dressing of claim 1 in the preparation of a medicament for the prevention and treatment of human papillomavirus infection.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108272915A (en) * | 2017-11-30 | 2018-07-13 | 康凤岐 | A kind of pharmaceutical composition and its preparation and purposes |
| CN111420028A (en) * | 2020-03-04 | 2020-07-17 | 桂林医学院 | Gel dressing for preventing and treating human papilloma virus infection and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108272915A (en) * | 2017-11-30 | 2018-07-13 | 康凤岐 | A kind of pharmaceutical composition and its preparation and purposes |
| CN111420028A (en) * | 2020-03-04 | 2020-07-17 | 桂林医学院 | Gel dressing for preventing and treating human papilloma virus infection and preparation method thereof |
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Application publication date: 20201127 |
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