CN111989406A - 一种测序文库的构建方法 - Google Patents

一种测序文库的构建方法 Download PDF

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Publication number
CN111989406A
CN111989406A CN201980013343.2A CN201980013343A CN111989406A CN 111989406 A CN111989406 A CN 111989406A CN 201980013343 A CN201980013343 A CN 201980013343A CN 111989406 A CN111989406 A CN 111989406A
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polynucleotide
tail
sequencing
substrate
dna
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CN111989406B (zh
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张翼
陈琼
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Beijing Youle Fusheng Technology Co ltd
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Beijing Youle Fusheng Technology Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

一种用于第二代高通量测序文库构建的方法和试剂盒，可提高核酸模板的利用效率，简化测序文库的构建流程，使得测序结果更加准确和覆盖度更加均一。

Description

PCT国内申请，说明书已公开。

Claims (29)

  1. PCT国内申请，权利要求书已公开。
CN201980013343.2A 2018-10-11 2019-08-26 一种测序文库的构建方法 Active CN111989406B (zh)

Applications Claiming Priority (3)

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CN201811185409 2018-10-11
CN201811185409X 2018-10-11
PCT/CN2019/102651 WO2020073748A1 (zh) 2018-10-11 2019-08-26 一种测序文库的构建方法

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CN111989406A true CN111989406A (zh) 2020-11-24
CN111989406B CN111989406B (zh) 2023-02-21

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US (1) US20220002713A1 (zh)
EP (1) EP3865584A4 (zh)
CN (1) CN111989406B (zh)
WO (1) WO2020073748A1 (zh)

Cited By (1)

* Cited by examiner, † Cited by third party
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CN113897414A (zh) * 2021-10-11 2022-01-07 湖南大地同年生物科技有限公司 一种痕量核酸文库构建方法

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113322523B (zh) * 2021-06-17 2024-03-19 翌圣生物科技(上海)股份有限公司 Rna快速建库方法及其应用

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US20100221785A1 (en) * 2005-05-26 2010-09-02 Human Genetic Signatures Pty Ltd Isothermal Strand Displacement Amplification Using Primers Containing a Non-Regular Base
CN104395480A (zh) * 2012-03-13 2015-03-04 斯威夫特生物科学公司 用于通过核酸聚合酶对衬底多核苷酸进行大小受控的同聚物加尾的方法和组合物
CN108085315A (zh) * 2016-11-21 2018-05-29 深圳华大基因科技有限公司 一种用于无创产前检测的文库构建方法及试剂盒
CN108456713A (zh) * 2017-11-27 2018-08-28 天津诺禾致源生物信息科技有限公司 接头封闭序列、文库构建试剂盒及测序文库的构建方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105525357B (zh) * 2014-09-30 2018-08-21 深圳华大基因股份有限公司 一种测序文库的构建方法及试剂盒和应用
WO2019055819A1 (en) * 2017-09-14 2019-03-21 Grail, Inc. METHODS FOR PREPARING A SEQUENCING LIBRARY FROM SINGLE STRANDED DNA
CN108004301B (zh) * 2017-12-15 2022-02-22 格诺思博生物科技南通有限公司 基因目标区域富集方法及建库试剂盒

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100221785A1 (en) * 2005-05-26 2010-09-02 Human Genetic Signatures Pty Ltd Isothermal Strand Displacement Amplification Using Primers Containing a Non-Regular Base
CN104395480A (zh) * 2012-03-13 2015-03-04 斯威夫特生物科学公司 用于通过核酸聚合酶对衬底多核苷酸进行大小受控的同聚物加尾的方法和组合物
CN108085315A (zh) * 2016-11-21 2018-05-29 深圳华大基因科技有限公司 一种用于无创产前检测的文库构建方法及试剂盒
CN108456713A (zh) * 2017-11-27 2018-08-28 天津诺禾致源生物信息科技有限公司 接头封闭序列、文库构建试剂盒及测序文库的构建方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ERWIN L VAN DIJK等: "Library preparation methods for next-generation sequencing:Tone down the bias", 《EXP CELL RES.》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113897414A (zh) * 2021-10-11 2022-01-07 湖南大地同年生物科技有限公司 一种痕量核酸文库构建方法

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Publication number Publication date
EP3865584A4 (en) 2021-12-08
EP3865584A1 (en) 2021-08-18
CN111989406B (zh) 2023-02-21
WO2020073748A1 (zh) 2020-04-16
US20220002713A1 (en) 2022-01-06

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