CN111983248A - Pesticide residue detector and corresponding detection method - Google Patents
Pesticide residue detector and corresponding detection method Download PDFInfo
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- CN111983248A CN111983248A CN202010825912.8A CN202010825912A CN111983248A CN 111983248 A CN111983248 A CN 111983248A CN 202010825912 A CN202010825912 A CN 202010825912A CN 111983248 A CN111983248 A CN 111983248A
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- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1004—Cleaning sample transfer devices
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- G01—MEASURING; TESTING
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- G01N2035/00465—Separating and mixing arrangements
- G01N2035/00534—Mixing by a special element, e.g. stirrer
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
- G01N2035/0401—Sample carriers, cuvettes or reaction vessels
- G01N2035/0418—Plate elements with several rows of samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
- G01N2035/0401—Sample carriers, cuvettes or reaction vessels
- G01N2035/0429—Sample carriers adapted for special purposes
- G01N2035/0432—Sample carriers adapted for special purposes integrated with measuring devices
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
- G01N2035/0401—Sample carriers, cuvettes or reaction vessels
- G01N2035/0429—Sample carriers adapted for special purposes
- G01N2035/0434—Sample carriers adapted for special purposes in the form of a syringe or pipette tip
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Abstract
A pesticide residue detector comprises a sample pretreatment module, a sample pretreatment module and a weighing module, wherein the sample pretreatment module comprises a sample rack and a weighing component positioned below the sample rack, a plurality of sample tube hole sites are arranged on the sample rack, and sample tubes are placed in the sample tube hole sites; the signal acquisition module is used for carrying out spectrophotometry detection on the reaction cup; the needle module is used for controlling the movement of the sample adding needle in the detector and the suction and discharge of the solution; the weighing assembly drives the weighing platform to jack up the sample tubes from the bottoms of the sample tubes through the XY direction sliding assembly, and then weighing is completed. After the pesticide residue detector and the detection method are adopted, automatic processing and automatic sampling detection before detection of the fruit and vegetable sample can be realized, the workload of detection personnel is reduced, the working efficiency is improved, and particularly in rapid detection of food safety.
Description
Technical Field
The invention relates to a pesticide residue detector and a corresponding detection method, which are mainly applied to the field of rapid detection of food safety.
Background
In the prior art, in the detection process, a detection person needs to manually pre-process a sample to be detected outside a spectrophotometry detector in advance, manually place the processed sample to be detected in a sample rack of the detector, then start the detector, correspondingly add a sample solution and a reagent into a reaction cup in sequence according to a test flow according to whether the detector is an automatic instrument or a semi-automatic instrument, and finally obtain a spectrophotometry detection result. Therefore, when a large number of fruit and vegetable samples are to be analyzed, the sample processing step is time-consuming, labor-consuming and error-prone.
According to the national standard GB/T5009.199-2003 rapid detection of organophosphorus and carbamate pesticide residue in vegetables, the sample processing step of the section 12.1 of the enzyme inhibition ratio method (spectrophotometry) requires: after the fruit and vegetable pesticide residue sample to be detected is processed, surface soil is manually removed and cut into pieces with the square length of about 1cm, 1g of the sample is weighed and taken, the sample is placed into a beaker or an extraction bottle, 5mL of buffer solution is added, oscillation is carried out for 1-2 min, an extracting solution is poured out, and standing is carried out for standby.
The following problems occur in the above operation steps:
when 1g of sample is sampled, the electronic balance is inconvenient to weigh and operate, the actual mass value is not easy to control, and both the operating efficiency and the test accuracy are not easy to consider;
when a plurality of samples to be tested are detected in the same batch, the testing personnel often do not vibrate sufficiently or even do not vibrate, and the elution effect of pesticide residue is influenced;
when a plurality of samples to be detected are detected in the same batch, the liquid to be detected is eluted and kept still for waiting time, the supernatant liquid of the plurality of samples to be detected after being kept still is sequentially transferred by manual operation, the sample adding gun head needs to be repeatedly replaced for cross contamination among the samples, the sample solution is repeatedly sucked, and the like, so that the workload of detection personnel is increased. In addition, during manual operation, the actual sucking position of the pipette head is not easy to control, the situation of sucking fruit and vegetable sample debris residues exists, sample adding errors easily occur due to manual repeated sample sucking and adding, and the accuracy of the fruit and vegetable pesticide residue detection result can be caused by the artificial factors.
In chinese patent document CN110907443A, a pesticide residue detector is disclosed, which comprises a pretreatment box, a sample measurement component and a base, wherein the pretreatment box pretreats vegetables, fruits or tea leaves to be detected, so that the vegetables, fruits or tea leaves to be detected can be crushed in the pretreatment box and dissolved in pesticide residue buffer solution, and then the crushed vegetables, fruits or tea leaves are fully mixed with the pesticide residue buffer solution to obtain a mixed solution. However, the sample processing step is not carried out according to the national standard, the function of the crushed sample in the patent can influence the accuracy of the detection result, and the juice contains plant secondary substances, such as chlorophyll, anthocyanin and the like which influence enzymes, so that the interference on the enzyme inhibitor is generated, false positive is easily generated, and the pesticide residue detection result is subjected to false detection; in addition, only one sample can be processed in each batch, efficient detection cannot be simultaneously performed when a plurality of groups of samples are tested, frequent operation is required for many times, cross contamination in pipeline channels is obvious, and time is consumed, so that the working efficiency of detection personnel is reduced.
Disclosure of Invention
The invention aims to provide a pesticide residue detector and a corresponding detection method, the detector can automatically carry out pretreatment on a fruit and vegetable sample, can automatically measure pesticide residues in the fruit and vegetable sample, and meets the requirement of quick detection of a farmer market.
In order to realize the invention, the invention provides a pesticide residue detector, which comprises a sample pretreatment module, a sample pretreatment module and a weighing module, wherein the sample pretreatment module comprises a sample rack and a weighing component positioned below the sample rack; the signal acquisition module is used for carrying out spectrophotometry detection on the reaction cup; the needle module is used for controlling the movement of the sample adding needle in the detector and the suction and discharge of the solution; the weighing assembly drives the weighing platform to jack up the sample tubes from the bottoms of the sample tubes through the XY direction sliding assembly, and then weighing is completed.
Furthermore, the sample pretreatment module further comprises an oscillation assembly, the oscillation assembly comprises a vibration motor and an oscillation support, and the sample holder is fixed on the upper side of the oscillation support.
Still further, the sample holder comprises a sample holder upper shell, a sample holder lower shell, and a sample tube fastener assembly; the sample tube clamping assembly comprises an asynchronous motor, a bolt and a clamping piece connected with the bolt, the clamping piece is movably arranged between the upper sample frame shell and the lower sample frame shell, and a plurality of sample tube hole sites are correspondingly arranged on the clamping piece; the sample tube is placed in the sample tube hole, the asynchronous motor drives the bolt and the clamping piece through a screw pair to generate relative motion with the upper sample rack shell and the lower sample rack shell, and the sample tube is clamped or loosened; when weighing, sample pipe chucking subassembly loosens the sample pipe, and when starting the oscillation, sample pipe chucking subassembly chucking sample pipe.
Furthermore, a sample tube detection assembly is further arranged in the sample tube hole site on the sample frame, and the sample tube detection assembly detects whether a sample tube is placed in the sample tube hole site corresponding to the sample tube detection assembly.
Furthermore, the signal acquisition module comprises a pesticide residue detection module, the pesticide residue detection module comprises a photoelectric detection module and a reaction cup array, a plurality of reaction cups are arranged in the reaction cup array, and the number of the reaction cups is consistent with the number of the sample tube holes.
Furthermore, the signal acquisition module further comprises a non-pesticide residue detection module.
Further, the needle module comprises a sample injection needle moving assembly and a sample injection needle, wherein the sample injection needle moving assembly comprises a moving assembly moving along X, Y and Z direction.
Furthermore, the moving component for Z-direction movement is a belt transmission component.
Further, the sample injection needle assembly comprises a large liquid gauge needle assembly and a small liquid gauge needle assembly, and the large liquid gauge needle assembly and the small liquid gauge needle assembly are respectively arranged on two sides of the belt transmission assembly.
The invention also provides a detection method for the detector, which comprises the steps that the sample tube detection assembly detects whether a sample tube is placed in the corresponding sample tube hole, if so, the system drives the weighing assembly to be placed below the sample tube for weighing, and the system adds a proper amount of buffer solution according to the weight of the sample tube; if not, the system drives the weighing component to skip the sample tube without weighing.
The invention also provides a detection method for the detector, which comprises the following steps of driving the weighing component to weigh the sample tube added with the sample to be detected on the sample rack; according to the weighing result, driving the needle module to add a certain amount of buffer solution into the sample tube, and driving the oscillation assembly to oscillate and uniformly mix the sample tube; after the sample is fully soaked, driving the needle module to extract supernatant from the sample tube and filling the supernatant into the reaction cup; the needle module is driven to extract a reagent from the reagent rack, fill the reagent into the reaction cup, suck, discharge and mix uniformly; the photoelectric detection module is driven to periodically monitor and interpret the reaction cup until the reaction is finished; and the system determines a pesticide residue detection result according to the monitoring and interpretation data.
After the pesticide residue detector and the detection method are adopted, automatic processing and automatic sampling detection before fruit and vegetable sample detection can be realized, a plurality of groups of fruit and vegetable samples can be processed and detected simultaneously, the operation of manually processing the samples before sample detection is cancelled, and the interference of human factors is reduced; the automatic pre-sample processing system adopts an oscillation elution method, thereby protecting the traceability of the fruit and vegetable sample to be detected, improving the accuracy of detection data, reducing the workload of detection personnel, improving the working efficiency, and being particularly used in the rapid detection of food safety.
Drawings
FIG. 1 is a block diagram of the whole structure of the full-automatic fruit and vegetable pesticide residue detector of the present invention;
FIG. 2 is a functional block diagram of the full-automatic fruit and vegetable pesticide residue detector of the present invention;
FIG. 3 is a structural block diagram of a sample pretreatment module of the full-automatic fruit and vegetable pesticide residue detector of the present invention;
FIG. 4 is a structural diagram of an oscillating assembly of the full-automatic fruit and vegetable pesticide residue detector of the present invention;
FIG. 5 is a schematic diagram of the structural modules of the sample tube gripping assembly;
FIG. 6 is a structural module diagram of a weighing assembly of the full-automatic fruit and vegetable pesticide residue detector of the present invention;
FIG. 7 is a block diagram of the pesticide residue detection module of the full-automatic fruit and vegetable pesticide residue detector of the present invention;
FIG. 8 is a structural diagram of a sample injection needle moving assembly of the full-automatic fruit and vegetable pesticide residue detector of the present invention;
FIG. 9 is a structural diagram of a lightweight sampling needle moving assembly of the full-automatic fruit and vegetable pesticide residue detector of the present invention;
FIG. 10 is a flow chart of the testing of the present invention.
Detailed Description
Specific embodiments of the present invention will be described below with reference to the accompanying drawings.
FIG. 1 is a block diagram of the whole automatic detector for pesticide residue in fruit and vegetable. As can be seen from the figure, the upper sampling needle movement assembly 1 includes a sampling needle and a movement assembly in which the sampling needle moves along X, Y and Z directions, wherein, the X, Y direction is a sliding assembly, the Z direction is a belt transmission assembly, the sampling needle movement assembly 1 is integrally fixed on the bracket 9 in a sliding manner, the base 5 is fixed with the sample pretreatment module 2, the pesticide residue detection module 3, the right side is the non-pesticide residue detection module 4, the reagent frame 6 and the power supply 8, the front side of the sample pretreatment module 2 is the pesticide residue detection module 3, the right side is the non-pesticide residue detection module 4, the rear side of the non-pesticide residue detection module 4 is the reagent frame 6, the rear side of the instrument is fixed with the injector assembly 7, and the power supply 8 is fixed under the injector assembly 7.
The sample adding needle moving assembly 1 is used for transferring the sample in the sample rack to the reaction assembly and transferring the reagent in the reagent rack to the reaction assembly, and automatically cleans the sample once every time the sample is sucked and discharged; the sample pretreatment module 2 is used for placing a sample tube and automatically finishing the pretreatment of the fruit and vegetable sample to be detected; the reaction assembly is used for carrying out specific reaction on fruit and vegetable samples and reagents and comprises a pesticide residue detection module 3 and a non-pesticide residue detection module 4, the pesticide residue detection module 3 is used for detecting special detection of pesticide residues such as organophosphorus and carbamic acid pesticides, and the non-pesticide residue detection module 4 is used for detecting physicochemical detection of other non-pesticide residues such as formaldehyde, sulfur and other physicochemical components; a base 5 for fixing each module; the reagent rack 6 is used for placing a reagent for detecting fruit and vegetable samples, and a sample adding needle cleaning tank is arranged beside the reagent rack and used for cleaning a sample adding needle so as to avoid cross contamination; the reagent rack 6 is also provided with a refrigerating assembly for storing the reagent under a certain temperature condition; the injector assembly 7 is used for controlling the washing liquid amount and the liquid discharge amount of the sample injection needle; the power supply 8 is used for supplying electric energy for the operation of the whole machine; and the bracket 9 is used for supporting the sample adding needle movement assembly.
The whole full-automatic pesticide residue instrument can be structurally divided into a sample area, a reagent area and a test area according to a main functional area, wherein the sample area is positioned in the area of the sample pretreatment module 2, mainly a sample cup is placed, and a weighing device is arranged below the sample area; the reagent area is positioned in the area of the reagent rack 6 and is mainly used for placing various reagents, such as dry powder dissolving solution, buffer solution, substrates and the like; the test area comprises a pesticide residue detection module 3 and a non-pesticide residue detection module 4, and is used for placing a reaction cup to incubate with reactants at constant temperature, and finally, reaction and signal acquisition are completed in the reaction cup.
FIG. 2 is a functional block diagram of the full-automatic fruit and vegetable pesticide residue detector of the present invention. The full-automatic pesticide residues are controlled by the PC, and the PC is connected with the sample pretreatment module, the needle module and the signal acquisition module in a communication mode to control the work of the sample pretreatment module, the needle module and the signal acquisition module. The sample pretreatment module comprises a sample rack, a weighing component and an oscillating component, wherein the weighing component and the oscillating component are positioned below the sample rack; the signal acquisition module comprises a photoelectric component which performs spectrophotometric detection on a reaction cup arranged in the pesticide residue detection module 3 area and a reaction cup arranged in the non-pesticide residue detection module 4 area in the test area in a moving mode and acquires photoelectric signals of the reaction cups; and the needle module is used for controlling the movement of the sample adding needle in the detector and the absorption and discharge of the solution.
FIG. 3 is a structural block diagram of a sample pretreatment module of the full-automatic fruit and vegetable pesticide residue detector of the present invention. As can be seen, the oscillating assembly 10 is located above the sample pre-treatment module 2 and the weighing assembly 11 is located below it. The oscillating assembly 10 is used for placing the sample tube and uniformly vibrating and mixing the sample tube through the vibrating module, and the weighing assembly 11 drives the weighing platform to jack up the sample tube from the bottom of the corresponding sample tube through the XY-direction sliding assembly to complete weighing.
Fig. 4 is a structural diagram of an oscillating assembly of the full-automatic fruit and vegetable pesticide residue detector of the invention. The oscillating assembly 10 comprises a vibrating motor 12, an oscillating support 14, a sample rack 15, a sample tube clamping assembly 13 and the like, wherein the vibrating motor 12 is fixed on the left side of the oscillating support 14, the sample rack 15 is fixed on the upper side of the oscillating support 14, and the sample tube clamping assembly 13 is fixed in the middle of the sample rack 15. The sample rack 15 is provided with 30 sample tube holes of 5 × 6 for placing sample tubes. Every sample tube hole site still is provided with sample tube detection subassembly for whether the sample tube hole site has placed the sample pipe. The sample tube detection component is usually an optical coupler, and can also be an elastic contact point and the like.
Fig. 5 is a schematic block diagram of the structure of the sample tube clamping assembly 13. The sample tube clamping assembly 13 comprises an asynchronous motor 17 and a bolt 18, and the sample rack consists of a sample rack lower shell 19, a clamping piece 20 and a sample rack upper shell 21. The asynchronous motor 17 is fixed with a bolt 18, the bolt 18 is fixed with a clamping piece 20 through a buckle, and the sample tube 16 is fixed through the clamping piece 20, the sample rack upper shell 21 and the sample rack lower shell 19. When the oscillation function is started, the pulse signal drives the asynchronous motor 17 to provide force in the F direction, the sample tube 16 is clamped tightly, the sample tube 16 is prevented from moving during oscillation, and the synchronous oscillation function of a plurality of groups of samples to be detected can be realized. The sample tube is loose when it is weighed and is clamped when it is oscillated.
FIG. 6 is a structural module diagram of a weighing assembly of the full-automatic fruit and vegetable pesticide residue detector of the present invention. As can be seen from the figure, a Y-direction sliding assembly and an X-direction sliding assembly are fixed on a base 23 of a weighing assembly, a weighing platform 29 is fixed on the upper side of the weighing assembly, a weighing sensor 28 is fixed on the lower side of the weighing platform 29, the weighing assembly is fixed on an X-direction sliding rod 26 through a sliding block 24, the weighing sensor 28 is pulled through an X-axis synchronous belt 27, the weighing platform 29 is enabled to weigh fruit and vegetable samples to be measured in the X-axis direction one by one, the X-direction sliding assembly is fixed on a Y-direction sliding rod 22 through a sliding block 24, the X-direction sliding assembly fixed on the Y-axis sliding rod 22 is enabled to move through pulling of the Y-axis synchronous belt 25, the weighing module is enabled to weigh fruit and vegetable samples to be measured. If the sample tube detection assembly detects that no sample tube is placed in the sample well site, the system will control the weighing assembly to skip weighing the well site. When the sample tube detection assembly detects that a weighing object is placed in the sample hole, the weighing sensor converts the weight of the weighing object into a digital signal; then the weight of the weighing object is output after being transmitted to a system for processing; the system adds the appropriate amount of buffer based on this weight. When the weight of the sample to be measured exceeds the specified weight, the system can prompt information to realize intelligent weighing control.
FIG. 7 is a block diagram of the pesticide residue detection module of the full-automatic fruit and vegetable pesticide residue detector of the present invention. The detection module comprises a photoelectric detection module 25, a reaction cup array 26, a reaction cup 27, a pesticide residue detection module bracket 28 and the like. The reaction cup array 26 is provided with a plurality of reaction cup placing positions, and each placing position is provided with a reaction cup detection assembly for detecting whether a reaction cup is placed on the placing position. The photoelectric detection module 25 moves along the arrangement direction of the reaction cup array 26, and measures once after passing through one reaction cup, and if the reaction cup detection assembly detects that no reaction cup is placed in the hole position of the reaction cup, the detection of the reaction cup is skipped.
The above-mentioned photoelectric detection module 25 is a single photoelectric module, and it is necessary to complete the detection of a plurality of cuvettes by means of movement. However, the motion detection method has various problems due to the existence of moving parts, such as: mechanical failure is easy to generate; the detection efficiency is low; the photoelectric component has long working time and is easy to age; and so on. The photoelectric detection module 25 can also be set to be in one-to-one correspondence with the reaction cup positions in the reaction cup array, so that a moving assembly is not needed, the detection of each reaction cup is carried out in real time, and the photoelectric detection process is stable and reliable.
For pesticide residue detection, the wavelength of the photoelectric detection module 25 is 410 nm; for multi-parameter, the wavelength can be set to multiple wavelengths according to the requirement of detection items.
FIG. 8 is a structural diagram of a sample injection needle moving assembly of the full-automatic fruit and vegetable pesticide residue detector of the present invention. The sample adding needle moving assembly comprises a sliding block 29, a motor 30, a small liquid needle assembly 31, a synchronous belt 32, a guide rail 33, a large liquid needle assembly 34 and the like. The small-volume needle assembly 31 is used for transporting a developing solution and the like having a small volume of 250. mu.l, and the large-volume needle assembly 34 is used for transporting a soak solution having a large volume of 10 ml, and the rear ends of the needles correspond to syringes having a corresponding volume standard. The two timing belts 32 drive the small fluid gauge needle assembly 31 and the large fluid gauge needle assembly 32 to move up and down, respectively.
Since the small capacity needle assembly 31 and the large capacity needle assembly 34 do not need to be raised or lowered at the same time, the needle assemblies may be disposed on both sides of one belt, and as shown in fig. 7, when the small capacity needle assembly 31 is raised, the large capacity needle assembly 34 is lowered; when the small capacity needle assembly 31 is lowered, the large capacity needle assembly 34 is raised.
The structure of the full-automatic pesticide residue detector of the invention is described in the foregoing, and the detection process is explained below.
FIG. 10 is a flow chart of the testing of the present invention. Firstly, setting a sample placed in a sample tube, wherein the system also provides a default sample setting mode, after the instrument detects that the sample tube is inserted into the sample frame, the instrument automatically finishes weighing, after the instrument detects that the weighing is finished, the instrument automatically moves to a reagent frame by a sample adding needle moving assembly, the sample adding needle downwards moves to detect the liquid level of a buffer solution and then absorbs the buffer solution with a certain proportion to the weight of the sample, after the instrument detects that the buffer solution is absorbed, the sample adding needle automatically resets, and the sample adding needle moving assembly automatically moves to the sample frame to add the absorbed buffer solution into the fruit and vegetable sample to be detected; after the instrument detects that the buffer solution is added into the fruit and vegetable sample to be detected, the sample adding needle moving assembly automatically moves to the cleaning groove to clean the sample adding needle; simultaneously, a vibration motor of an oscillation assembly of the sample processing module is automatically started to oscillate and mix the fruit and vegetable sample to be detected and the buffer solution uniformly, after the oscillation for a certain time, the vibration motor of the oscillation assembly automatically stops the oscillation, then the fruit and vegetable sample to be detected is fully soaked in the buffer solution, the residue of the fruit and vegetable sample to be detected sinks, and the solution rises to form supernatant; the device automatically completes the detection pretreatment function of the fruit and vegetable sample to be detected.
After the instrument detects that the fruit and vegetable sample to be detected is fully soaked, the sample adding needle moving assembly is automatically moved to a sample frame, the sample adding needle moves downwards to detect the liquid level of the supernatant of the fruit and vegetable sample to be detected and then automatically absorbs the supernatant of the fruit and vegetable sample to be detected, after the instrument detects that a certain amount of the supernatant is absorbed, the sample adding needle automatically resets, the sample adding needle moving assembly is automatically moved to a reaction cup, the supernatant absorbed by the sample adding needle is added into the reaction cup of the reaction cup, and after the instrument detects that the supernatant is added into the reaction cup, the sample adding needle moving assembly is automatically moved to a cleaning groove to clean the sample adding needle; after the instrument detects that the automatic cleaning of the sampling needle is finished, the sampling needle moving assembly automatically moves to a reagent rack, the sampling needle moves downwards to absorb a certain amount of reagent after detecting the liquid level of the reagent, after the detection that the sampling needle absorbs the reagent is finished, the sampling needle automatically resets, the sampling needle moving assembly automatically moves to a reaction cup, the reagent absorbed by the sampling needle is added into the reaction cup of the reaction cup, after the instrument detects that the reagent is added into the reaction cup, the sampling needle repeatedly absorbs and discharges liquid in the reaction cup for uniform mixing for a certain number of times, and after the uniform mixing is fully realized, the sampling needle moving assembly automatically moves to a cleaning groove to clean the sampling needle; the supernatant of the fruit and vegetable sample to be detected is fully reacted with the reagent, and the photoelectric detection module periodically monitors, judges and reads the numerical value of the reaction cup in the reaction process.
If the reaction cup is already provided with the dry powder reagent, the whole testing process needs to comprise a dry powder reagent dissolving step which needs to be completed before adding the reagent.
Finally, it should be noted that: the above embodiments are merely exemplary embodiments applicable to the conventional breadth for illustrating and not limiting the present invention, and those skilled in the art can make modifications or equivalent substitutions to the present invention without departing from the spirit and scope of the present invention, which shall be covered by the claims of the present invention.
Claims (11)
1. A pesticide residue detector comprises a sample pretreatment module, a sample pretreatment module and a weighing module, wherein the sample pretreatment module comprises a sample rack and a weighing component positioned below the sample rack, a plurality of sample tube hole sites are arranged on the sample rack, and sample tubes are placed in the sample tube hole sites; the signal acquisition module is used for carrying out spectrophotometry detection on the reaction cup; the needle module is used for controlling the movement of the sample adding needle in the detector and the suction and discharge of the solution; the weighing device is characterized in that the weighing assembly drives the weighing platform to jack up the sample tubes from the bottoms of the sample tubes through the XY direction sliding assembly, and then weighing is completed.
2. The apparatus according to claim 2, wherein the sample pre-treatment module further comprises an oscillating assembly (10), the oscillating assembly (10) comprising a vibrating motor (12) and an oscillating support (14), the sample holder being fixed to an upper side of the oscillating support (14).
3. The meter of claim 2, wherein the sample holder comprises a sample holder upper shell (21), a sample holder lower shell (19), and a sample tube clamp assembly; the sample tube clamping assembly comprises an asynchronous motor (17), a bolt (18) and a clamping piece (20) connected with the bolt, wherein the clamping piece (20) is movably arranged between the upper sample frame shell (21) and the lower sample frame shell (19), and a plurality of sample tube hole sites are correspondingly arranged on the clamping piece; the sample tube is placed in the sample tube hole, the asynchronous motor (17) drives the bolt (18) and the clamping piece (20) through a screw pair to generate relative motion with the sample frame upper shell (21) and the sample frame lower shell (19), and the sample tube is clamped or loosened; when weighing, sample pipe chucking subassembly loosens the sample pipe, and when starting the oscillation, sample pipe chucking subassembly chucking sample pipe.
4. The meter according to any one of claims 1 to 3, wherein a sample tube detection assembly is further provided in a sample tube hole site on the sample holder, and the sample tube detection assembly detects whether a sample tube is placed in the sample tube hole site corresponding to the sample tube detection assembly.
5. The detector according to claim 4, wherein the signal acquisition module comprises a pesticide residue detection module (3), the pesticide residue detection module comprises a photoelectric detection module and a reaction cup array, a plurality of reaction cups are arranged in the reaction cup array, and the number of the reaction cups is consistent with the number of the sample tube holes.
6. The detector according to claim 5, characterized in that the signal acquisition module further comprises a non-pesticide residue detection module, the non-pesticide residue detection module (4).
7. The meter of claim 4, wherein the needle module comprises a sample needle movement assembly and a sample needle, the sample needle movement assembly comprising a movement assembly that moves in directions X, Y and Z.
8. The meter of claim 7, wherein the Z-direction movement assembly is a belt drive assembly.
9. The meter of claim 8, wherein the needle assembly includes a large volume needle assembly and a small volume needle assembly, the large volume needle assembly and the small volume needle assembly being disposed on opposite sides of a belt of the belt drive assembly.
10. A method for detecting the detecting instrument according to any one of claims 4 to 9, wherein the method comprises the steps that the sample tube detecting component detects whether a sample tube is placed at the corresponding sample tube hole site, if so, the system drives the weighing component to the position below the sample tube for weighing, and the system adds an appropriate amount of buffer solution according to the weight of the sample tube; if not, the system drives the weighing component to skip the sample tube without weighing.
11. A testing method for use with the meter according to any one of claims 1 to 9, wherein the method comprises driving the weighing assembly to weigh a sample tube on the sample holder into which a sample to be tested is loaded; according to the weighing result, driving the needle module to add a certain amount of buffer solution into the sample tube, and driving the oscillation assembly to oscillate and uniformly mix the sample tube; after the sample is fully soaked, driving the needle module to extract supernatant from the sample tube and filling the supernatant into the reaction cup; the needle module is driven to extract a reagent from the reagent rack, fill the reagent into the reaction cup, suck, discharge and mix uniformly; the photoelectric detection module is driven to periodically monitor and interpret the reaction cup until the reaction is finished; and the system determines a pesticide residue detection result according to the monitoring and interpretation data.
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| CN113484314A (en) * | 2021-08-16 | 2021-10-08 | 智锐达仪器科技南通有限公司 | Detection method for pesticide residue detector and pesticide residue detector |
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| CN113484314A (en) * | 2021-08-16 | 2021-10-08 | 智锐达仪器科技南通有限公司 | Detection method for pesticide residue detector and pesticide residue detector |
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