CN111971394B - 嵌合抗原受体的肿瘤环境特异性表达 - Google Patents

嵌合抗原受体的肿瘤环境特异性表达 Download PDF

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CN111971394B
CN111971394B CN201980025929.0A CN201980025929A CN111971394B CN 111971394 B CN111971394 B CN 111971394B CN 201980025929 A CN201980025929 A CN 201980025929A CN 111971394 B CN111971394 B CN 111971394B
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tme
promoter
expression
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tumor
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CN111971394A (zh
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A·波加多
罗伊·加齐特
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National Institute for Biotechnology in the Negev Ltd
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CN201980025929.0A 2018-02-15 2019-02-14 嵌合抗原受体的肿瘤环境特异性表达 Expired - Fee Related CN111971394B (zh)

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US201862631095P 2018-02-15 2018-02-15
US62/631,095 2018-02-15
US201862784501P 2018-12-23 2018-12-23
US62/784,501 2018-12-23
PCT/IL2019/050182 WO2019159173A1 (en) 2018-02-15 2019-02-14 Tumor environment-specific expression of chimeric antigen receptors

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CN111971394B true CN111971394B (zh) 2024-05-28

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US (1) US20210030799A1 (https=)
EP (2) EP3619313B9 (https=)
JP (1) JP2021514187A (https=)
CN (1) CN111971394B (https=)
AU (1) AU2019221324A1 (https=)
CA (1) CA3093628A1 (https=)
IL (1) IL276636A (https=)
WO (1) WO2019159173A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021053678A1 (en) * 2019-09-22 2021-03-25 The National Institute for Biotechnology in the Negev Ltd. Tumor environment specific expression of effector genes
US20230364137A1 (en) * 2020-09-30 2023-11-16 Vor Biopharma Inc. Chimeric antigen receptor expression systems
CN118105504A (zh) * 2022-11-29 2024-05-31 深圳先进技术研究院 一种具有肿瘤微环境响应的乳酸杆菌载体及应用
CN118685362A (zh) * 2024-05-15 2024-09-24 西安电子科技大学 一种癌环境响应型的过继单核或巨噬细胞免疫治疗生物制品及其在抗肿瘤中的应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017133175A1 (en) * 2016-02-04 2017-08-10 Nanjing Legend Biotech Co., Ltd. Engineered mammalian cells for cancer therapy

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2017342176A1 (en) * 2016-10-10 2019-05-02 The National Institute for Biotechnology in the Negev Ltd. Non-cytotoxic modified cells and use thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017133175A1 (en) * 2016-02-04 2017-08-10 Nanjing Legend Biotech Co., Ltd. Engineered mammalian cells for cancer therapy

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
A Tet-On Inducible System for Controlling CD19-Chimeric Antigen Receptor Expression upon Drug Administration;Sakemura et al.;《Cancer Immunology Research》;第4卷(第8期);第OF1页摘要，OF2左栏，OF4右栏 *
Design and development of therapies using chimeric antigen receptor-expressing T cells;Dotti et al.;《Immunological Reviews》;第257卷;第121页右栏 *
Therapeutic Improvement of a Stroma-Targeted CRAd by Incorporating Motives Responsive to the Melanoma Microenvironment;Diego L. Viale et al.;《J Invest Dermatol.》;第133卷(第11期);第2576页摘要、第2577页左栏、右栏 *
使用热休克蛋白基因启动子调控靶基因在肿瘤局部表达;黄倩等;《中华病理学杂志》;第30卷(第3期);第198-201页 *

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EP3619313B9 (en) 2023-10-04
US20210030799A1 (en) 2021-02-04
CN111971394A (zh) 2020-11-20
AU2019221324A1 (en) 2020-09-03
EP3619313A1 (en) 2020-03-11
IL276636A (en) 2020-09-30
EP4279501A3 (en) 2024-02-28
JP2021514187A (ja) 2021-06-10
EP4279501A2 (en) 2023-11-22
WO2019159173A1 (en) 2019-08-22
CA3093628A1 (en) 2019-08-22
EP3619313B1 (en) 2023-06-07

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