CN111964942A - Sampling method based on industrial environment pollutant detection - Google Patents
Sampling method based on industrial environment pollutant detection Download PDFInfo
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- CN111964942A CN111964942A CN202010886901.0A CN202010886901A CN111964942A CN 111964942 A CN111964942 A CN 111964942A CN 202010886901 A CN202010886901 A CN 202010886901A CN 111964942 A CN111964942 A CN 111964942A
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- industrial
- solvent
- method based
- sampling method
- sampling
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- 239000003344 environmental pollutant Substances 0.000 title claims abstract description 47
- 238000005070 sampling Methods 0.000 title claims abstract description 38
- 231100000719 pollutant Toxicity 0.000 title claims abstract description 31
- 238000001514 detection method Methods 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 24
- 239000002904 solvent Substances 0.000 claims abstract description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 238000004925 denaturation Methods 0.000 claims abstract description 14
- 230000036425 denaturation Effects 0.000 claims abstract description 14
- 239000000243 solution Substances 0.000 claims abstract description 14
- 239000000356 contaminant Substances 0.000 claims abstract description 10
- 230000007613 environmental effect Effects 0.000 claims abstract description 7
- 239000011536 extraction buffer Substances 0.000 claims abstract description 6
- 239000002736 nonionic surfactant Substances 0.000 claims abstract description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 22
- 229910021389 graphene Inorganic materials 0.000 claims description 17
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 14
- 239000000344 soap Substances 0.000 claims description 14
- 238000004806 packaging method and process Methods 0.000 claims description 7
- 238000002791 soaking Methods 0.000 claims description 7
- 239000011780 sodium chloride Substances 0.000 claims description 7
- 229910052783 alkali metal Inorganic materials 0.000 claims description 5
- 150000001340 alkali metals Chemical class 0.000 claims description 5
- 229910052799 carbon Inorganic materials 0.000 claims description 5
- 125000004432 carbon atom Chemical group C* 0.000 claims description 5
- 239000011259 mixed solution Substances 0.000 claims description 5
- 239000002086 nanomaterial Substances 0.000 claims description 5
- 239000004094 surface-active agent Substances 0.000 claims description 5
- 238000002474 experimental method Methods 0.000 claims description 4
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 claims description 3
- 229910052784 alkaline earth metal Inorganic materials 0.000 claims description 3
- 150000001342 alkaline earth metals Chemical class 0.000 claims description 3
- 150000001412 amines Chemical class 0.000 claims description 3
- 239000003153 chemical reaction reagent Substances 0.000 claims 2
- 239000007788 liquid Substances 0.000 description 4
- 239000006185 dispersion Substances 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000010419 fine particle Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000001208 nuclear magnetic resonance pulse sequence Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a sampling method based on industrial environment pollutant detection, which comprises the following steps: sampling industrial environmental pollutants, so that a pollutant solvent for preventing denaturation is mixed with the industrial environmental pollutants, and the pollutant solvent for preventing denaturation is contacted with the industrial environmental pollutants in a closable mixing container, so that cold water which is contained in a film soluble in the solvent and is mixed with at least one extraction buffer solution, particularly nonionic surfactant, serving as the solvent is contacted in the mixing container for later use; encapsulating the contaminants, using the contaminant solvents for preventing denaturation and industrial environmental contaminants. The sampling method based on the industrial environment pollutant detection can keep the internal molecular structure of the sample to be detected to the maximum extent, so that the accuracy of later detection is maximized, and meanwhile, the sampling method is pollution-free, environment-friendly and has a good prospect.
Description
Technical Field
The invention relates to the field of chemical sampling, in particular to a sampling method based on industrial environment pollutant detection.
Background
Sampling means that a signal sample sequence at regular intervals is used for replacing an original continuous signal in time, namely, an analog signal is discretized in time, and the process of converting the continuous signal into the discrete signal is called a sampling process, wherein the sampling process is realized by a sampling switch (sampler), and the physical meaning of the sampling process is a process that a unit ideal pulse sequence T (t) is subjected to amplitude adjustment by an input signal f (t);
at present, the stability of a molecular structure of a collected sample cannot be guaranteed to the maximum in a sampling process, and certain hidden danger is caused to the accuracy of later detection, so that a sampling method based on industrial environment pollutant detection is provided.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides the following technical scheme: the sampling method based on industrial environmental pollutant detection comprises the following steps:
step one, sampling industrial environment pollutants, so that a pollutant solvent for preventing denaturation is mixed with the industrial environment pollutants, and the pollutant solvent for preventing denaturation is contacted with the industrial environment pollutants in a closable mixing container, so that cold water which is contained in a film soluble in the solvent and mixed with at least one extraction buffer solution, particularly nonionic surfactant, serving as the solvent is contacted in the mixing container for later use;
step two, packaging pollutants, namely dripping the pollutant solvent for preventing denaturation and industrial environment pollutants into the surface of one graphene sheet, and covering the other graphene sheet on the surface of the graphene sheet to finish packaging;
step three, refreshing, namely completely soaking the packaged mixed solution in 20-40% sodium chloride solution for 5-7min, wherein the water temperature for soaking is 15-19 ℃ for later use;
and step four, taking out the fresh-keeping sampling product and carrying out detection or experiment on the fresh-keeping sampling product.
Preferably, the concentration of the sodium chloride solution is 5%.
Preferably, the graphene is a two-dimensional carbon nanomaterial formed by composing carbon atoms into hexagonal honeycomb lattices by sp hybridized orbitals.
Preferably, the temperature of the cold water is from 0 to 20 ℃.
Preferably, the preservative agent is a free DNA preservative agent.
Preferably, the surfactant can be any one of alkali metal soap, alkaline earth metal soap, organic amine soap and triethanolamine soap.
Compared with the prior art, the invention has the following beneficial effects: the sampling method based on the industrial environment pollutant detection can keep the internal molecular structure of the sample to be detected to the maximum extent, so that the accuracy of later detection is maximized, and meanwhile, the sampling method is pollution-free, environment-friendly and has a good prospect.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The sampling method based on the industrial environmental pollutant detection is characterized in that the industrial environmental pollutant is taken during sampling, sampling industrial environmental pollutants to mix a pollutant solvent for preventing denaturation with the industrial environmental pollutants, and prevents the denatured contaminant solvent from contacting the industrial environmental contaminant in a closable mixing vessel, bringing cold water, which is contained in a solvent-soluble film and contains an extraction buffer solution, as a solvent, in which at least one surfactant, particularly a nonionic surfactant, selected from any one of alkali metal soaps, alkaline earth metal soaps, organic amine soaps, and triethanolamine soaps, is incorporated, into contact in a mixing vessel, and uniformly dispersing insoluble solid matter in the liquid as fine particles to form a system called dispersion or suspension, which is called dispersion, and the temperature of the cold water is 0 to 20 ℃ for later use; dropwise adding the to-be-packaged liquid to the surface of one piece of graphene, and then covering the other piece of graphene, wherein the graphene is a two-dimensional carbon nanomaterial with carbon atoms forming hexagonal honeycomb lattices by sp hybridized orbits, and packaging is completed; completely soaking the packaged mixed solution in 20-40% sodium chloride solution with concentration of 5% and water temperature of 15-19 deg.C for 5-7 min; the fresh-keeping sampling sample is taken out to be detected or tested, the internal molecular structure of the sampling sample to be detected can be kept to the maximum extent, the accuracy of later detection is maximized, and meanwhile, the sampling method is pollution-free and environment-friendly.
Example 1
Sampling industrial environmental pollutants, so that a pollutant solvent for preventing denaturation is mixed with the industrial environmental pollutants, and the pollutant solvent for preventing denaturation is contacted with the industrial environmental pollutants in a closable mixing container, so that cold water which is contained in a film soluble in the solvent and is mixed with at least one extraction buffer solution, particularly a nonionic surfactant, as the solvent is contacted in the mixing container, wherein the surfactant is selected from alkali metal soap, and the temperature of the cold water is 20 ℃ for standby; dropwise adding the to-be-packaged liquid to the surface of one piece of graphene, and then covering the other piece of graphene, wherein the graphene is a two-dimensional carbon nanomaterial with carbon atoms forming hexagonal honeycomb lattices by sp hybridized orbits, and packaging is completed; completely soaking the packaged mixed solution in 40% sodium chloride solution with concentration of 5% and water temperature of 19 deg.C for 7 min; taking out the fresh-keeping sample and carrying out detection or experiment on the fresh-keeping sample;
through detection, compared with field collection, the molecular structure stability of industrial environmental pollutants reaches 98%, and the water loss is only 0.02%.
Example 2
Sampling industrial environmental pollutants, so that a pollutant solvent for preventing denaturation is mixed with the industrial environmental pollutants, and the pollutant solvent for preventing denaturation is contacted with the industrial environmental pollutants in a closable mixing container, so that cold water which is contained in a film soluble in the solvent and is mixed with at least one extraction buffer solution, particularly a nonionic surfactant, as the solvent is contacted in the mixing container, wherein the surfactant is selected from alkali metal soap, and the temperature of the cold water is 20 ℃ for standby; dropwise adding the to-be-packaged liquid to the surface of one piece of graphene, and then covering the other piece of graphene, wherein the graphene is a two-dimensional carbon nanomaterial with carbon atoms forming hexagonal honeycomb lattices by sp hybridized orbits, and packaging is completed; completely soaking the packaged mixed solution in 50% sodium chloride solution with concentration of 5% and water temperature of 21 deg.C for 8 min; taking out the fresh-keeping sample and carrying out detection or experiment on the fresh-keeping sample;
through detection, compared with field collection, the molecular structure stability of industrial environmental pollutants reaches 99%, and the water loss is only 0.03%.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. The sampling method based on industrial environment pollutant detection is characterized by comprising the following steps:
step one, sampling industrial environment pollutants, so that a pollutant solvent for preventing denaturation is mixed with the industrial environment pollutants, and the pollutant solvent for preventing denaturation is contacted with the industrial environment pollutants in a closable mixing container, so that cold water which is contained in a film soluble in the solvent and mixed with at least one extraction buffer solution, particularly nonionic surfactant, serving as the solvent is contacted in the mixing container for later use;
step two, packaging pollutants, namely dripping the pollutant solvent for preventing denaturation and industrial environment pollutants into the surface of one graphene sheet, and covering the other graphene sheet on the surface of the graphene sheet to finish packaging;
step three, refreshing, namely completely soaking the packaged mixed solution in 20-40% sodium chloride solution for 5-7min, wherein the water temperature for soaking is 15-19 ℃ for later use;
and step four, taking out the fresh-keeping sampling product and carrying out detection or experiment on the fresh-keeping sampling product.
2. The sampling method based on industrial environmental contaminant detection according to claim 1, wherein: the concentration of the sodium chloride solution was 5%.
3. The sampling method based on industrial environmental contaminant detection according to claim 1, wherein: graphene is a two-dimensional carbon nanomaterial consisting of carbon atoms in sp hybridized orbitals in a hexagonal honeycomb lattice.
4. The sampling method based on industrial environmental contaminant detection according to claim 1, wherein: the temperature of the cold water is 0-20 ℃.
5. The sampling method based on industrial environmental contaminant detection according to claim 1, wherein: the fresh-keeping reagent is a free DNA fresh-keeping reagent.
6. The sampling method based on industrial environmental contaminant detection according to claim 1, wherein: the surfactant can be any one of alkali metal soap, alkaline earth metal soap, organic amine soap, and triethanolamine soap.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010886901.0A CN111964942A (en) | 2020-08-28 | 2020-08-28 | Sampling method based on industrial environment pollutant detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010886901.0A CN111964942A (en) | 2020-08-28 | 2020-08-28 | Sampling method based on industrial environment pollutant detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111964942A true CN111964942A (en) | 2020-11-20 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010886901.0A Pending CN111964942A (en) | 2020-08-28 | 2020-08-28 | Sampling method based on industrial environment pollutant detection |
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|---|---|---|---|---|
| CN101909756A (en) * | 2007-11-20 | 2010-12-08 | 3M创新有限公司 | Sample preparation and gathering system and method |
| CN201724839U (en) * | 2010-03-22 | 2011-01-26 | 杨扬 | Special portable and moving tool box for sampling water |
| CN101963549A (en) * | 2009-07-22 | 2011-02-02 | 中国科学院大气物理研究所 | Collection method of fully automatic dustfall collector |
| CN103124564A (en) * | 2010-03-22 | 2013-05-29 | 弗·哈夫曼-拉罗切有限公司 | Compositions and methods useful for stabilizing protein-containing formulations |
| CN106153395A (en) * | 2016-09-21 | 2016-11-23 | 中国地质大学（北京） | A kind of integrated sampling apparatus of subsoil water |
| CN106659208A (en) * | 2014-06-12 | 2017-05-10 | 爱尔伯股份公司 | Method for extracting contaminants from agrarian products and apparatus therefor |
| CN106769287A (en) * | 2016-11-30 | 2017-05-31 | 北京大学 | A kind of method that utilization Graphene monocrystalline support membrane contained network realizes high efficiency liquid-packing |
| CN109406215A (en) * | 2018-12-28 | 2019-03-01 | 南京大学 | A kind of water body environment DNA intelligent acquisition device and acquisition method |
| CN111108363A (en) * | 2017-09-21 | 2020-05-05 | 贝克顿·迪金森公司 | Reactivity partition template for harmful pollutant test |
| CN111133113A (en) * | 2017-09-21 | 2020-05-08 | 贝克顿·迪金森公司 | High dynamic range assay in hazardous contaminants testing |
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2020
- 2020-08-28 CN CN202010886901.0A patent/CN111964942A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101909756A (en) * | 2007-11-20 | 2010-12-08 | 3M创新有限公司 | Sample preparation and gathering system and method |
| CN101963549A (en) * | 2009-07-22 | 2011-02-02 | 中国科学院大气物理研究所 | Collection method of fully automatic dustfall collector |
| CN201724839U (en) * | 2010-03-22 | 2011-01-26 | 杨扬 | Special portable and moving tool box for sampling water |
| CN103124564A (en) * | 2010-03-22 | 2013-05-29 | 弗·哈夫曼-拉罗切有限公司 | Compositions and methods useful for stabilizing protein-containing formulations |
| CN106659208A (en) * | 2014-06-12 | 2017-05-10 | 爱尔伯股份公司 | Method for extracting contaminants from agrarian products and apparatus therefor |
| CN106153395A (en) * | 2016-09-21 | 2016-11-23 | 中国地质大学（北京） | A kind of integrated sampling apparatus of subsoil water |
| CN106769287A (en) * | 2016-11-30 | 2017-05-31 | 北京大学 | A kind of method that utilization Graphene monocrystalline support membrane contained network realizes high efficiency liquid-packing |
| CN111108363A (en) * | 2017-09-21 | 2020-05-05 | 贝克顿·迪金森公司 | Reactivity partition template for harmful pollutant test |
| CN111133113A (en) * | 2017-09-21 | 2020-05-08 | 贝克顿·迪金森公司 | High dynamic range assay in hazardous contaminants testing |
| CN109406215A (en) * | 2018-12-28 | 2019-03-01 | 南京大学 | A kind of water body environment DNA intelligent acquisition device and acquisition method |
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Application publication date: 20201120 |
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