CN111944885B - Cloning method of Pinus massoniana miRNA precursor gene - Google Patents
Cloning method of Pinus massoniana miRNA precursor gene Download PDFInfo
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- CN111944885B CN111944885B CN202010375167.1A CN202010375167A CN111944885B CN 111944885 B CN111944885 B CN 111944885B CN 202010375167 A CN202010375167 A CN 202010375167A CN 111944885 B CN111944885 B CN 111944885B
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- precursor gene
- masson pine
- cloning
- mirna precursor
- mir172
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/40—Afforestation or reforestation
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Abstract
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Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN202010375167.1A CN111944885B (en) | 2020-05-06 | 2020-05-06 | Cloning method of Pinus massoniana miRNA precursor gene |
Applications Claiming Priority (1)
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CN202010375167.1A CN111944885B (en) | 2020-05-06 | 2020-05-06 | Cloning method of Pinus massoniana miRNA precursor gene |
Publications (2)
Publication Number | Publication Date |
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CN111944885A CN111944885A (en) | 2020-11-17 |
CN111944885B true CN111944885B (en) | 2023-03-14 |
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CN202010375167.1A Active CN111944885B (en) | 2020-05-06 | 2020-05-06 | Cloning method of Pinus massoniana miRNA precursor gene |
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Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN115927377B (en) * | 2022-09-22 | 2023-08-08 | 北京林业大学 | Wet-added pine age marker gene PtAP2L3, and screening method and application thereof |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104164425A (en) * | 2014-07-10 | 2014-11-26 | 东北农业大学 | miRNA, precursor of miRNA, their coding gene and application thereof |
CN104611325A (en) * | 2014-12-31 | 2015-05-13 | 国际竹藤中心 | Cloning method of microRNA precursor genes of moso bamboos |
CN110157707A (en) * | 2019-05-27 | 2019-08-23 | 中国农业科学院作物科学研究所 | The application of one rice miRNA and its precursor-gene in rice bacterial blight resistance |
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2020
- 2020-05-06 CN CN202010375167.1A patent/CN111944885B/en active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104164425A (en) * | 2014-07-10 | 2014-11-26 | 东北农业大学 | miRNA, precursor of miRNA, their coding gene and application thereof |
CN104611325A (en) * | 2014-12-31 | 2015-05-13 | 国际竹藤中心 | Cloning method of microRNA precursor genes of moso bamboos |
CN110157707A (en) * | 2019-05-27 | 2019-08-23 | 中国农业科学院作物科学研究所 | The application of one rice miRNA and its precursor-gene in rice bacterial blight resistance |
Non-Patent Citations (3)
Title |
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"Over-expression of miR172 causes loss of spikelet determinacy and floral organ abnormalities in rice (Oryza sativa)";Qian-Hao Zhu 等;《BMC Plant Biology volume》;20091217;全文 * |
"日本落叶松种子萌发过程中18个miRNAs的表达变化";吴涛 等;《林业科学研究》;20131231;摘要 * |
拟南芥miR395d基因的克隆和过表达载体构建及其在油菜中的转化;张芸等;《南京农业大学学报》;20100330(第02期);全文 * |
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Inventor after: Xu Meng Inventor after: Ye Youju Inventor after: Xu Lian Inventor after: Ni Zhouxian Inventor after: Han Xin Inventor after: Xin Yue Inventor after: Tian Yating Inventor before: Xu Lian Inventor before: Ye Youju Inventor before: Xu Meng Inventor before: Ni Zhouxian Inventor before: Han Xin Inventor before: Xin Yue Inventor before: Tian Yating |
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