CN111938230B - Respiratory tract transmitted pathogen enrichment spinning membrane, sampling mask paster and sampling mask - Google Patents
Respiratory tract transmitted pathogen enrichment spinning membrane, sampling mask paster and sampling mask Download PDFInfo
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- CN111938230B CN111938230B CN202010548759.9A CN202010548759A CN111938230B CN 111938230 B CN111938230 B CN 111938230B CN 202010548759 A CN202010548759 A CN 202010548759A CN 111938230 B CN111938230 B CN 111938230B
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- A—HUMAN NECESSITIES
- A41—WEARING APPAREL
- A41D—OUTERWEAR; PROTECTIVE GARMENTS; ACCESSORIES
- A41D13/00—Professional, industrial or sporting protective garments, e.g. surgeons' gowns or garments protecting against blows or punches
- A41D13/05—Professional, industrial or sporting protective garments, e.g. surgeons' gowns or garments protecting against blows or punches protecting only a particular body part
- A41D13/11—Protective face masks, e.g. for surgical use, or for use in foul atmospheres
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- A—HUMAN NECESSITIES
- A41—WEARING APPAREL
- A41D—OUTERWEAR; PROTECTIVE GARMENTS; ACCESSORIES
- A41D27/00—Details of garments or of their making
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B32—LAYERED PRODUCTS
- B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- B32B5/00—Layered products characterised by the non- homogeneity or physical structure, i.e. comprising a fibrous, filamentary, particulate or foam layer; Layered products characterised by having a layer differing constitutionally or physically in different parts
- B32B5/02—Layered products characterised by the non- homogeneity or physical structure, i.e. comprising a fibrous, filamentary, particulate or foam layer; Layered products characterised by having a layer differing constitutionally or physically in different parts characterised by structural features of a fibrous or filamentary layer
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B32—LAYERED PRODUCTS
- B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- B32B5/00—Layered products characterised by the non- homogeneity or physical structure, i.e. comprising a fibrous, filamentary, particulate or foam layer; Layered products characterised by having a layer differing constitutionally or physically in different parts
- B32B5/22—Layered products characterised by the non- homogeneity or physical structure, i.e. comprising a fibrous, filamentary, particulate or foam layer; Layered products characterised by having a layer differing constitutionally or physically in different parts characterised by the presence of two or more layers which are next to each other and are fibrous, filamentary, formed of particles or foamed
- B32B5/24—Layered products characterised by the non- homogeneity or physical structure, i.e. comprising a fibrous, filamentary, particulate or foam layer; Layered products characterised by having a layer differing constitutionally or physically in different parts characterised by the presence of two or more layers which are next to each other and are fibrous, filamentary, formed of particles or foamed one layer being a fibrous or filamentary layer
- B32B5/26—Layered products characterised by the non- homogeneity or physical structure, i.e. comprising a fibrous, filamentary, particulate or foam layer; Layered products characterised by having a layer differing constitutionally or physically in different parts characterised by the presence of two or more layers which are next to each other and are fibrous, filamentary, formed of particles or foamed one layer being a fibrous or filamentary layer another layer next to it also being fibrous or filamentary
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B32—LAYERED PRODUCTS
- B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- B32B2262/00—Composition or structural features of fibres which form a fibrous or filamentary layer or are present as additives
- B32B2262/08—Animal fibres, e.g. hair, wool, silk
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B32—LAYERED PRODUCTS
- B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- B32B2307/00—Properties of the layers or laminate
- B32B2307/70—Other properties
- B32B2307/714—Inert, i.e. inert to chemical degradation, corrosion
- B32B2307/7145—Rot proof, resistant to bacteria, mildew, mould, fungi
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B32—LAYERED PRODUCTS
- B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- B32B2307/00—Properties of the layers or laminate
- B32B2307/70—Other properties
- B32B2307/724—Permeability to gases, adsorption
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B32—LAYERED PRODUCTS
- B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- B32B2535/00—Medical equipment, e.g. bandage, prostheses, catheter
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B32—LAYERED PRODUCTS
- B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- B32B2571/00—Protective equipment
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- General Health & Medical Sciences (AREA)
- Physical Education & Sports Medicine (AREA)
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Abstract
The invention discloses a respiratory tract transmitted pathogen enrichment spinning membrane, a sampling mask paster and a sampling mask. The enrichment spinning membrane is composed of a protein spinning membrane, has the characteristics of multi-layer porosity and air permeability, is coupled with and captures an antibody of a respiratory tract transmitted pathogen, can efficiently block external air and the pathogen exhaled from the inside of a human body through the specific combination of the pathogen and the antibody, and realizes bidirectional protection; the sampling mask provided by the invention can capture pathogens in breath of a mask wearer while blocking the pathogens, so that the pathogens can be enriched in a patch to the maximum extent, sample collection in a long-distance travel process is facilitated, a complex throat swab process is avoided, doctors and patients are protected, detection pressure is relieved, and effectiveness of subsequent detection and patient investigation efficiency are improved.
Description
Technical Field
The invention relates to the field of biomedical materials, in particular to a respiratory tract transmitted pathogen enrichment spinning film, a sampling mask paster and a sampling mask.
Background
Respiratory infectious diseases refer to diseases caused by invasion of pathogens such as viruses, bacteria, mycoplasma and chlamydia from respiratory infections such as nasal cavity, throat, trachea and bronchus of human body, and include influenza, measles, chicken pox, rubella, epidemic encephalitis, mumps, pulmonary tuberculosis, etc. The disease is often characterized by strong infectivity, multiple transmission ways, high transmission speed, wide transmission range, common and easy crowd and the like, and becomes one of the most harmful infectious diseases.
Throughout the development history of infectious diseases, the epidemic situation of respiratory infectious diseases is not interrupted, and because of the characteristics of strong infectivity and the like, if the infectious diseases are improperly controlled, the large-scale spread is easily caused in a short time, the human health is influenced, and even social panic is caused. In order to maintain the steady development of society, the control of respiratory infectious diseases is indispensable.
The infectious chain of respiratory infections mainly consists of three factors: the infection source: primarily patients or occult infected; the transmission path is as follows: including droplet nuclear transmission, aerosol transmission, contact transmission, and the like; susceptible people: the immunity to infection is lacked, and the people are easy to be infected. The destruction of the infectious chain is the main control principle of respiratory infectious diseases, and the wearing of a mask to filter air entering a human body to block a transmission path is one of important measures for preventing the respiratory infectious diseases. The National Institute of Occupational Safety and Health (NIOSH) and the world health organization recommend N95 medical protective masks to be used for preventing and treating respiratory infectious diseases, but most hospitals in China currently mainly wear gauze masks and surgical masks even respiratory infectious disease hospitals, but the masks only have a certain mechanical blocking effect, cannot effectively block pathogens from invading through the respiratory tract, only play a role in simple protection and still have a certain infection risk.
On the other hand, the nucleic acid detection is a common means for detecting respiratory infectious diseases at present due to the characteristics of high sensitivity, good specificity, good timeliness, strong stability and the like. However, when the throat swab collection is carried out on a patient, the throat part needs to be exposed by mouth opening, and exhaled gas is likely to be aerosol and spray containing viruses, so that the risk of throat swab collection workers is extremely high. Meanwhile, one-time sampling requires 3-10 minutes on average, and is easily influenced by human factors, so that the missed diagnosis of positive cases and even serious consequences of spreading of epidemic situation of respiratory infectious diseases are caused.
Disclosure of Invention
The invention aims to solve the technical problems in the prior art and provides a respiratory tract transmitted pathogen enrichment spinning membrane, a sampling mask paster and a sampling mask.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows: a respiratory-transmitted pathogen-enriched spun film is provided that includes a protein spun film coupled to a capture antibody that is a specific antibody capable of targeting binding to a plurality of respiratory-transmitted pathogens.
Preferably, the respiratory transmitted pathogens include, but are not limited to, influenza virus, coronavirus, measles virus, respiratory syncytial virus, meningococcus, mycobacterium tuberculosis.
Preferably, the protein spinning membrane includes, but is not limited to, a natural plant protein membrane, a plant protein spinning membrane, an animal protein spinning membrane, and an insect spinning membrane.
Preferably, the preparation of the respiratory tract transmitted pathogen enriched spun film comprises the steps of: preparing a protein spinning membrane mouse-derived monoclonal antibody and a pathogen specificity mouse-derived monoclonal antibody, and bridging the protein spinning membrane mouse-derived monoclonal antibody and the pathogen specificity mouse-derived monoclonal antibody through an anti-mouse secondary antibody to obtain the respiratory tract transmitted pathogen enriched spinning membrane.
Preferably, the preparation method of the protein spinning membrane comprises the following steps: cutting the protein spinning net into squares with the same size, and vertically overlapping and laying the squares into a non-woven fabric base material; then evenly spraying water mist, placing the non-woven fabric substrate in the middle of a hot pressing plate, and carrying out vertical hot pressing to form the protein spinning film with the thickness of 0.5mm +/-0.05 mm and the porosity of 2-5%.
Preferably, the method for preparing the protein-spun membrane murine monoclonal antibody comprises the following steps: immunizing a mouse with the protein spinning membrane antigen, taking a spleen cell of the mouse, fusing with a myeloma cell of the mouse, and screening a monoclonal antibody hybridoma secreting a specific anti-protein spinning membrane antigen to obtain a stable hybridoma cell strain; then, the cell is inoculated to the abdominal cavity of a mouse, ascites is prepared, and the specific protein spinning membrane mouse-derived monoclonal antibody is extracted from the ascites.
Preferably, the method for producing the pathogen-specific murine monoclonal antibody comprises the steps of: immunizing a mouse with the pathogen specific antigen, taking spleen cells of the mouse, fusing with myeloma cells of the mouse, and screening monoclonal antibody hybridoma cells secreting the specific anti-pathogen antigen to obtain a stable hybridoma cell strain; then, the cell is inoculated to the abdominal cavity of a mouse, ascites is prepared, and a specific pathogen-specific murine monoclonal antibody is extracted from the ascites.
Preferably, the preparation method of the anti-mouse secondary antibody comprises the following steps: the gamma globulin immune goat, horse, donkey, rabbit or chicken of the mouse is subjected to a certain immune procedure, animal serum is collected, and a specific anti-mouse antibody in the serum is extracted.
The invention also provides a respiratory tract transmitted pathogen enrichment sampling mask paster which is characterized in that the respiratory tract transmitted pathogen enrichment sampling mask paster is prepared by attaching a bonding layer to one side of the respiratory tract transmitted pathogen enrichment spinning film.
The invention also provides a respiratory tract transmitted pathogen enrichment sampling mask which is characterized in that the sampling mask patch is arranged on the inner side of the mask.
The invention has the beneficial effects that:
1. the respiratory tract transmitted pathogen enrichment spinning membrane is composed of a protein spinning membrane, has the characteristics of multi-layer porosity and air permeability, is coupled with and captures an antibody of the respiratory tract transmitted pathogen, can efficiently block external air and the pathogen exhaled from the inside of a human body through the specific combination of the pathogen and the antibody, and realizes bidirectional protection;
2. the mask paster can be matched with a traditional mask for use, does not influence the wearing and using mode of the original mask, and can be matched with the mechanical blocking effect of the traditional mask to realize physical and biological dual protection; the mask patch is convenient to use and wide in applicability, and the used materials are healthy and environment-friendly and do not influence normal wearing;
3. the respiratory tract transmitted pathogen enrichment sampling mask provided by the invention can capture pathogens in the breath of a mask wearer while blocking the pathogens, so that the pathogens can be enriched to the maximum extent in a patch, sample collection in a long-distance travel process is facilitated, a complex throat swab process is avoided, doctors and patients are protected, the detection pressure is relieved, and the effectiveness of subsequent detection and the patient investigation efficiency are improved.
Drawings
FIG. 1 is a functional schematic diagram of a respiratory tract pathogen enrichment sampling mask patch of the present invention;
FIG. 2 is a schematic structural view of the respiratory tract pathogen enrichment sampling mask of the present invention;
FIG. 3 is a chart of a colorimetric chart for color development in example 1 of the present invention;
FIG. 4 is an SDS-PAGE electrophoretogram of the monoclonal antibody in example 2 of the present invention;
FIG. 5 is a graph showing the results of ELISA titer detection of monoclonal antibodies in example 2 of the present invention.
Description of the reference numerals:
1, a mask; 2-respiratory tract transmitted pathogen enrichment sampling mask paster.
Detailed Description
The present invention is further described in detail below with reference to examples so that those skilled in the art can practice the invention with reference to the description.
It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
The present example provides a respiratory-transmitted pathogen-enriched spun membrane comprising a protein spun membrane coupled to a capture antibody, the capture antibody being a specific antibody capable of targeting binding to a plurality of respiratory-transmitted pathogens.
Wherein the respiratory pathogens include, but are not limited to, influenza virus, coronavirus, measles virus, respiratory syncytial virus, meningococcus, and mycobacterium tuberculosis.
Wherein, the protein spinning membrane comprises but is not limited to natural plant protein membrane, plant protein spinning membrane, animal protein spinning membrane and insect spinning membrane.
The preparation method of the respiratory tract transmitted pathogen enrichment spinning membrane comprises the following steps: preparing a protein spinning membrane murine monoclonal antibody and a pathogen specific murine monoclonal antibody, and bridging the protein spinning membrane murine monoclonal antibody and the pathogen specific murine monoclonal antibody through an anti-murine secondary antibody to obtain a spinning membrane with a function of capturing respiratory tract transmitted pathogens: i.e., respiratory tract transmitted pathogen-enriched spun membranes.
The embodiment also provides a respiratory tract transmitted pathogen enrichment sampling mask patch and a sampling mask prepared according to the respiratory tract transmitted pathogen enrichment spinning film. In the scheme of the invention, by simultaneously capturing and enriching pathogens in the breath of a mask wearer, a complex throat swab process can be avoided, doctors and patients can be protected, and the effectiveness of subsequent detection and the investigation efficiency of the patients can be improved.
The present invention is further described in detail below with reference to the following examples.
Example 1
The preparation of the protein spinning membrane comprises the following steps:
1. protein spinning
Freezing plant protein filaments, animal protein filaments and insect spinning filaments, grinding into ultra-short filaments or powder, adding a small amount of polyethylene terephthalate (PET), fully and uniformly mixing, and then spinning, filament falling and drafting to form a protein spinning net with the aperture of 50 mu m +/-10 mu m.
2. Machining
Cutting the protein spinning net into squares with the same size, and vertically overlapping and laying the squares into a non-woven fabric base material; evenly spraying water mist, placing the non-woven fabric substrate in the middle of a hot-pressing plate, and carrying out vertical hot pressing to form a protein spinning film with the thickness of 0.5mm +/-0.05 mm and the porosity of 2-5%.
3. Protein spinning membrane assay
The pore size, thickness and porosity of the protein spinning membrane were assayed and subjected to indirect ELISA assays using specific monoclonal antibodies. The ELISA assay procedure was as follows: adding a protein spinning membrane into a 24-pore plate, then adding an anti-protein spinning membrane antibody with the final concentration of 15 mu g/ml, incubating at room temperature for 2h, washing with PBST washing liquor for 3 times, then adding an enzyme-labeled anti-mouse secondary antibody, reacting at 37 ℃ for 30min, washing with PBST washing liquor for 5 times, adding a precipitable substrate TMB for color development for 15min, detecting the color development condition of a protein spinning membrane, and determining that the color development grade is not less than 5, thus the product is qualified. The color chart is shown in FIG. 3.
Example 2
Preparation of murine monoclonal antibodies and anti-murine secondary antibodies:
1. preparation of a murine monoclonal antibody comprising the steps of:
1. immunization of mice
Respectively preparing protein spinning membrane antigen and pathogen antigen, emulsifying, and performing multiple-point immunization on BLAB/C mice via abdominal cavity, dorsal subcutaneous route, etc., for 4 times with interval period of 10 days. The immunization was boosted 2 days before the fusion by the rat tail vein.
2. Cell fusion
Taking the immunized mouse spleen, preparing a spleen cell suspension, and mixing the mouse spleen cell with the cultured NS1 myeloma cell. Mouse spleen cells and NS1 myeloma cells were fused using 50% of PEG 4000 as a fusing agent. The fusion cells were cultured for several days in selection medium containing 20% newborn calf serum.
3. Cloning and screening
The protein spinning membrane antigen and the pathogen antigen are respectively operated according to the following steps: diluting to 10 μ g/ml with 0.05M carbonate buffer solution with pH9.6, and coating with 100 μ L/hole of ELISA plate; overnight at 4 ℃; washing with 0.03-0.06% Tween-20 in Phosphate Buffer (PBST) for 5 times, and draining; adding the cell culture supernatant to be detected, incubating at 37 ℃, and detecting by adopting an indirect ELISA method. The Cutoff value was 2.1 times the negative control value, and a positive result was obtained when the Cutoff value was higher than the negative control value. Selecting the fusion cells in the positive holes of the detection result, and performing multiple times of monoclonal culture by a limiting dilution method; and (4) measuring after the single cell grows into the subclone, and freezing and storing by liquid nitrogen after the positive clone is enlarged.
4. Preparation and purification of monoclonal antibody
Culturing the hybridoma cell strain, injecting into the abdominal cavity of sensitized mice, each 10 times 6 (ii) individual cells; observing the abdominal cavity of the mouse, and collecting ascites of the mouse within about 5 to 10 days; slowly adding saturated ammonium sulfate solution with equal proportion into ascites of the mouse while stirring, stirring for 1h, and standing at 4 ℃ overnight; centrifuging the mouse ascites after precipitation, collecting the precipitate, and dissolving the precipitate with a small amount of physiological saline; then removing ammonium sulfate in the solution by using a G-25 gel chromatographic column; purifying the monoclonal antibody by using a DEAE-52 anion exchange chromatography column, and eluting by using NaCl solutions with different concentrations to obtain a protein spinning membrane mouse-derived monoclonal antibody and a pathogen specificity mouse-derived monoclonal antibody; the purity and titer of the purified antibody are determined by SDS-PAGE electrophoresis and indirect ELISA method, and the detection results are shown in figure 4 and figure 5, which shows that the purity and titer of the antibody obtain good results.
2. The preparation of the anti-mouse secondary antibody comprises the following steps: the gamma globulin immune goat, horse, donkey, rabbit or chicken of a mouse is subjected to a certain immune procedure, animal serum is collected, and a specific anti-mouse antibody in the serum is extracted.
Example 3
The preparation of the respiratory tract transmitted pathogen enrichment spinning film, the mask paster and the sampling mask comprises the following steps:
1. protein spinning membrane linked pathogen specific monoclonal antibody
The protein spinning membrane is respectively soaked in an anti-protein spinning membrane specific antibody solution with the final concentration of 10-30 mug/ml and stays overnight at 4 ℃. Washing with 0.03-0.06% Tween-20 Phosphate Buffer Solution (PBST) for 5 times, detecting by indirect ELISA, soaking the protein spinning membrane in 10-30 μ g/ml anti-mouse secondary antibody solution, and standing overnight at 4 deg.C. The next day PBST washing solution is washed for 5 times, then is soaked in an anti-pathogen specific monoclonal antibody solution with the final concentration of 10-30 mu g/ml, is incubated and washed according to the method, and is detected by an ELISA method.
2. Protein spinning membrane slice
Draining the protein spinning membrane connected with the pathogen specific monoclonal antibody, mechanically cutting and processing to prepare a circular or square respiratory tract transmitted pathogen enrichment spinning membrane with the diameter of 10mm +/-2 mm.
3. Preparation of mask paster
Attaching a sticking layer such as an environment-friendly adhesive or a self-adhesive to one surface of the prepared protein spinning membrane body to obtain the respiratory tract transmitted pathogen enrichment sampling mask patch, and referring to fig. 1.
4. Preparation of sampling mask
Referring to fig. 2, the mask patch 2 is adhered to the inner side of the mask 1 to obtain the respiratory tract transmitted pathogen enrichment sampling mask.
While embodiments of the invention have been disclosed above, it is not intended to be limited to the details shown in the description and the examples, which are set forth, but are fully applicable to various fields of endeavor as are suited to the particular use contemplated, and further modifications will readily occur to those skilled in the art, since the invention is not limited to the details shown and described without departing from the general concept as defined by the appended claims and their equivalents.
Claims (7)
1. A respiratory-transmitted pathogen-enriched spun film comprising a protein spun film coupled to a capture antibody, the capture antibody being a specific antibody capable of targeting binding to a plurality of respiratory-transmitted pathogens;
the protein spinning membrane is a combination of a plant protein spinning membrane, an animal protein spinning membrane and an insect spinning membrane;
the preparation steps of the respiratory tract transmitted pathogen enrichment spinning membrane are as follows: preparing a protein spinning membrane mouse-derived monoclonal antibody and a pathogen specificity mouse-derived monoclonal antibody, and bridging the protein spinning membrane mouse-derived monoclonal antibody and the pathogen specificity mouse-derived monoclonal antibody through an anti-mouse secondary antibody to obtain a respiratory tract transmitted pathogen enriched spinning membrane;
the preparation method of the protein spinning membrane comprises the following steps: cutting the protein spinning net into squares with the same size, and vertically overlapping and laying the squares into a non-woven fabric base material; then evenly spraying water mist, placing the non-woven fabric substrate in the middle of a hot-pressing plate, and carrying out vertical hot-pressing to form a protein spinning film with the thickness of 0.5mm +/-0.05 mm and the porosity of 2-5%.
2. The respiratory pathogen-enriched spun film of claim 1, wherein the respiratory pathogen comprises at least influenza virus, coronavirus, measles virus, respiratory syncytial virus, meningococcus, and tubercle bacillus.
3. The respiratory tract transmitted pathogen-enriched spun film according to claim 2, wherein the preparation method of the protein spun film murine monoclonal antibody comprises the following steps: immunizing a mouse with the protein spinning membrane antigen, taking a spleen cell of the mouse, fusing with a myeloma cell of the mouse, and screening a monoclonal antibody hybridoma secreting a specific anti-protein spinning membrane antigen to obtain a stable hybridoma cell strain; then, the cell is inoculated to the abdominal cavity of a mouse, ascites is prepared, and the specific protein spinning membrane mouse-derived monoclonal antibody is extracted from the ascites.
4. The respiratory tract-transmitted pathogen-enriched spinning membrane according to claim 3, wherein the method for preparing the pathogen-specific murine monoclonal antibody comprises the steps of: immunizing a mouse with a pathogen specific antigen, taking a spleen cell of the mouse, fusing with a myeloma cell of the mouse, and screening a monoclonal antibody hybridoma secreting the specific anti-pathogen antigen to obtain a stable hybridoma cell strain; then, the cells are inoculated into the abdominal cavity of a mouse, ascites is prepared, and a specific pathogen-specific murine monoclonal antibody is extracted from the ascites.
5. The respiratory tract transmissible pathogen enriching spinning membrane according to claim 4, wherein the preparation method of the anti-mouse secondary antibody comprises the following steps: the gamma globulin immune goat, horse, donkey, rabbit or chicken of a mouse is subjected to a certain immune procedure, animal serum is collected, and a specific anti-mouse antibody in the serum is extracted.
6. A respiratory tract transmitted pathogen enrichment sampling mask patch which is characterized in that the respiratory tract transmitted pathogen enrichment sampling mask patch is prepared by attaching the respiratory tract transmitted pathogen enrichment spinning membrane of any one of claims 1-5 to a single surface of an adhesive layer.
7. A respiratory tract transmission pathogen enrichment sampling mask, which is characterized in that the inner side of the mask is provided with the sampling mask paster of claim 6.
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| TWM619782U (en) * | 2020-10-06 | 2021-11-21 | 康科特股份有限公司 | Facial mask for collecting virus specimen from respirator tracts of human and animals |
| CN113519939B (en) * | 2021-09-17 | 2021-12-28 | 中南大学 | Mouth-nose mask for visible target collection of novel coronavirus sample and visible warning |
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| CN104560874A (en) * | 2014-12-04 | 2015-04-29 | 南昌大学 | Method for capturing memory B cells aimed at respiratory syncytial virus by virtue of Elisa method |
| CN106422522A (en) * | 2015-08-11 | 2017-02-22 | 清华大学 | Silk nano-fiber-based air filtering device |
| CN108037290A (en) * | 2017-11-13 | 2018-05-15 | 深圳市博卡生物技术有限公司 | A kind of kit and its detection method of the detection of respiratory pathogen antigen |
| CN111227347A (en) * | 2020-02-23 | 2020-06-05 | 刘巍 | Special enrichment sampling medical mask for respiratory tract transmitted pathogens |
| CN210445776U (en) * | 2020-02-29 | 2020-05-05 | 首都医科大学宣武医院 | Mask for sampling pathogen of coronavirus pneumonia |
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